JPS63112532A - Preventing method for clouding or precipitating of glutaraldehyde aqueous solution - Google Patents
Preventing method for clouding or precipitating of glutaraldehyde aqueous solutionInfo
- Publication number
- JPS63112532A JPS63112532A JP25531386A JP25531386A JPS63112532A JP S63112532 A JPS63112532 A JP S63112532A JP 25531386 A JP25531386 A JP 25531386A JP 25531386 A JP25531386 A JP 25531386A JP S63112532 A JPS63112532 A JP S63112532A
- Authority
- JP
- Japan
- Prior art keywords
- aqueous solution
- sodium
- glutaraldehyde
- stabilizer
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007864 aqueous solution Substances 0.000 title claims abstract description 43
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims description 12
- 230000001376 precipitating effect Effects 0.000 title abstract 3
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims abstract description 18
- 239000003381 stabilizer Substances 0.000 claims abstract description 17
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 14
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 claims abstract description 12
- 229940001584 sodium metabisulfite Drugs 0.000 claims abstract description 12
- 235000010262 sodium metabisulphite Nutrition 0.000 claims abstract description 12
- 235000010265 sodium sulphite Nutrition 0.000 claims abstract description 9
- 239000004471 Glycine Substances 0.000 claims abstract description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 6
- 125000003172 aldehyde group Chemical group 0.000 claims abstract description 6
- LCPVQAHEFVXVKT-UHFFFAOYSA-N 2-(2,4-difluorophenoxy)pyridin-3-amine Chemical compound NC1=CC=CN=C1OC1=CC=C(F)C=C1F LCPVQAHEFVXVKT-UHFFFAOYSA-N 0.000 claims abstract description 4
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims abstract description 4
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims abstract description 4
- CHQMHPLRPQMAMX-UHFFFAOYSA-L sodium persulfate Substances [Na+].[Na+].[O-]S(=O)(=O)OOS([O-])(=O)=O CHQMHPLRPQMAMX-UHFFFAOYSA-L 0.000 claims abstract description 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims abstract description 3
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 3
- VLSOAXRVHARBEQ-UHFFFAOYSA-N [4-fluoro-2-(hydroxymethyl)phenyl]methanol Chemical compound OCC1=CC=C(F)C=C1CO VLSOAXRVHARBEQ-UHFFFAOYSA-N 0.000 claims abstract description 3
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 3
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 3
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 3
- 235000003704 aspartic acid Nutrition 0.000 claims abstract description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 3
- XWGJFPHUCFXLBL-UHFFFAOYSA-M rongalite Chemical compound [Na+].OCS([O-])=O XWGJFPHUCFXLBL-UHFFFAOYSA-M 0.000 claims abstract description 3
- 125000000524 functional group Chemical group 0.000 claims abstract 3
- GRWZHXKQBITJKP-UHFFFAOYSA-L dithionite(2-) Chemical compound [O-]S(=O)S([O-])=O GRWZHXKQBITJKP-UHFFFAOYSA-L 0.000 claims abstract 2
- 238000001556 precipitation Methods 0.000 claims description 11
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 abstract description 12
- 229910000030 sodium bicarbonate Inorganic materials 0.000 abstract description 6
- 235000017557 sodium bicarbonate Nutrition 0.000 abstract description 6
- 239000000645 desinfectant Substances 0.000 abstract description 2
- 229940079827 sodium hydrogen sulfite Drugs 0.000 abstract description 2
- 230000002070 germicidal effect Effects 0.000 abstract 2
- JPYHHZQJCSQRJY-UHFFFAOYSA-N Phloroglucinol Natural products CCC=CCC=CCC=CCC=CCCCCC(=O)C1=C(O)C=C(O)C=C1O JPYHHZQJCSQRJY-UHFFFAOYSA-N 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- QCDYQQDYXPDABM-UHFFFAOYSA-N phloroglucinol Chemical compound OC1=CC(O)=CC(O)=C1 QCDYQQDYXPDABM-UHFFFAOYSA-N 0.000 abstract 1
- 229960001553 phloroglucinol Drugs 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 12
- 239000000126 substance Substances 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 6
- 229940079877 pyrogallol Drugs 0.000 description 6
- 230000002265 prevention Effects 0.000 description 5
- 239000011521 glass Substances 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 4
- 229940001482 sodium sulfite Drugs 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 239000013068 control sample Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229960002449 glycine Drugs 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical group OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- -1 hydrocarphite Chemical compound 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 241000256215 Spongomorpha aeruginosa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- DANUORFCFTYTSZ-UHFFFAOYSA-N epinigericin Natural products O1C2(C(CC(C)(O2)C2OC(C)(CC2)C2C(CC(O2)C2C(CC(C)C(O)(CO)O2)C)C)C)C(C)C(OC)CC1CC1CCC(C)C(C(C)C(O)=O)O1 DANUORFCFTYTSZ-UHFFFAOYSA-N 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 229940079826 hydrogen sulfite Drugs 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- DANUORFCFTYTSZ-BIBFWWMMSA-N nigericin Chemical compound C([C@@H]1C[C@H]([C@H]([C@]2([C@@H](C[C@](C)(O2)C2O[C@@](C)(CC2)C2[C@H](CC(O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C)O1)C)OC)[C@H]1CC[C@H](C)C([C@@H](C)C(O)=O)O1 DANUORFCFTYTSZ-BIBFWWMMSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229940068984 polyvinyl alcohol Drugs 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
本発明はグルタールアルデヒド水溶液を殺菌消毒剤とし
て使用する１祭に認められる濁りもしくは沈澱の現象を
防止することを目的とする安定化剤を提供する方法に関
するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for providing a stabilizing agent aimed at preventing the phenomena of turbidity or precipitation observed when using an aqueous glutaraldehyde solution as a disinfectant. .
近年、グルタールアルデヒドは、アミン基、水酸基等に
対する高い反応性に基づき、皮革のなめし剤、蛋白の架
橋剤、酸素の固・定化剤、写真用フィルムの現像剤に用
いられて来た。特に近年血清肝炎、敗血症など種々のウ
ィルス、細石芽胞、グラム陰性菌による院内感染に対し
、グルタールアルデヒドの化学的滅菌又は消毒作用への
評価は高く、その需要は急速に増大しつつある。この際
、グルタールアルデヒドは約２％の濃度の水溶液として
用いられ、殺菌効力を高めるため炭酔水素ナトリウム等
を添加し、水溶液のｐｉ（を７５〜８５８度の弱アルカ
リ性になる様に調節される（例えば、米国特許第３［］
１１６３２８号参照。この様にグルタールアルデヒド水
溶液のｐＨを弱アルカＩＪ 性に調節した場合、室温も
しくはそれ以上の温度柔性で水溶液を放置すると、水溶
液中に濁りあるいは沈澱といった現象の生ずることが、
しばしば認められることがある。この様な水溶液中に発
生した固体物質の、例えば極めて高価な内視鏡又は内視
鏡消毒装置などの医療用器具等の間隙への混入あるいけ
表面への付着といったことが生じた場合、その除去に対
する時間の労費、あるいは除去不能に基づく器具の分解
修理もしくは廃棄につながり、非常に不経済であると共
に、付着物が医療器具に残った場合には、その使用対象
となる患者への危険性が高まることになる。In recent years, glutaraldehyde has been used as a tanning agent for leather, a crosslinking agent for proteins, an agent for fixing and fixing oxygen, and a developer for photographic film, based on its high reactivity with amine groups, hydroxyl groups, etc. Particularly in recent years, glutaraldehyde has been highly evaluated for its chemical sterilization or disinfection effects against hospital infections caused by various viruses, microspores, and Gram-negative bacteria, such as serum hepatitis and sepsis, and its demand is rapidly increasing. At this time, glutaraldehyde is used as an aqueous solution with a concentration of about 2%, and sodium bicarbonate, etc. is added to increase the bactericidal efficacy, and the pi (pi) of the aqueous solution is adjusted to be slightly alkaline at 75 to 858 degrees. (e.g., U.S. Pat. No. 3 []
See No. 116328. In this way, when the pH of the glutaraldehyde aqueous solution is adjusted to be weakly alkaline, phenomena such as turbidity or precipitation may occur in the aqueous solution if the aqueous solution is left at room temperature or higher temperature flexibility.
often recognized. If a solid substance generated in such an aqueous solution gets mixed into the gap or adheres to the surface of an extremely expensive endoscope or medical equipment such as an endoscope sterilizer, This is very uneconomical as it requires time and labor costs for removal, or the device must be disassembled for repair or discarded if it cannot be removed, and if the deposit remains on the medical device, it poses a danger to the patient for whom the device is being used. Sexuality will increase.
この様な事情からグルタールアルデヒド水溶液を弱アル
カリ性に調節した際に認められる濁りもしくは沈澱の発
生を防止することが要望されていたＯ
本発明者らは今回、グルタールアルデヒド水溶液を弱ア
ルカリ性に調節する時に成る種の物質を同時に添加する
ことにより、水溶液の中に濁りもしくは沈澱が認められ
なくなることを発見した。Due to these circumstances, it has been desired to prevent the occurrence of turbidity or precipitation that is observed when a glutaraldehyde aqueous solution is adjusted to a weak alkalinity. It has been discovered that by simultaneously adding a variety of substances to the aqueous solution, no turbidity or precipitate is observed in the aqueous solution.
従って本発明は弱アルカリ性に調節したグルタールアル
デヒド水溶液中にしばしば認められるで蜀りもしくは沈
澱現象を防止する安定化剤を提供することを目的とする
。Accordingly, an object of the present invention is to provide a stabilizer that prevents the sludge or precipitation phenomenon often observed in aqueous solutions of glutaraldehyde adjusted to be slightly alkaline.
本発明の安定化剤はアルデヒド基と反応性を有する物質
のうち、特にその構造中に亜硫酸基、アミ７基あるいは
水酸基を有する物質の１種または２種以上より成るもの
である。The stabilizer of the present invention is composed of one or more substances having a sulfite group, an amide group, or a hydroxyl group in its structure, among substances that are reactive with aldehyde groups.
この様にアルデヒド基と反応する物質を添００すること
により、グルタールアルデヒド水溶液の殺菌効力がグル
タールアルデヒド濃度に依存することから、殺菌効力の
低下につながることが危傾されるが、本発明の様に添加
する量がグルタールアルデヒド濃度Ｑて対し約１０〜と
低くかつグルタールアルデヒドがアルデヒド基を１分子
中に２個有する２官能基物質であることから、計算上反
応により消失する量はその半分即ち約５％となり、実用
上の視点からすれば殺菌効力の低下はほとんど認められ
ない。By adding a substance that reacts with aldehyde groups in this way, the bactericidal efficacy of the glutaraldehyde aqueous solution depends on the glutaraldehyde concentration, so there is a risk that the bactericidal efficacy will decrease. Since the amount added is as low as about 10 to 10% relative to the glutaraldehyde concentration Q, and glutaraldehyde is a bifunctional substance with two aldehyde groups in one molecule, the calculated amount disappears by the reaction. is half of that amount, or about 5%, and from a practical point of view, there is hardly any decrease in bactericidal efficacy.
本発明に使用される安定化剤の例として亜硫酸水素ナト
リウム、亜硫酸す）・リウム、ロンガリソ寸
ト、ハイドロカルファイト、メタ重亜硫酸ナトリウム、
過硫酸ナトリウム、ヒドロキシンミン塩酸塩、グリシン
、アスパラギン酸、Ｌ−システイン塩酸塩、アスコルピ
ン酸、ポリビニルアルコール、ピロガロール、フェロロ
グルシノール’４をｌ’ることができ、特に好ましくは
該化合物は亜硫酸水素ナトリウム、亜硫酸ナトリウム、
ロンガリット、ハイドロザルファイト、メタ重亜伽酸ナ
トリウム、過硫酸ナトリウムである。Examples of stabilizers used in the present invention include sodium hydrogen sulfite, sulfite, chloride, hydrocarphite, sodium metabisulfite,
Sodium persulfate, hydroxymine hydrochloride, glycine, aspartic acid, L-cysteine hydrochloride, ascorbic acid, polyvinyl alcohol, pyrogallol, ferroroglucinol, particularly preferably the compound is hydrogen sulfite. sodium, sodium sulfite,
Rongalite, hydrozulfite, sodium metabicarbonate, and sodium persulfate.
本発明における安定化剤のグルタールアルデヒド水溶液
への添ノーロ方去は、粉末においては水溶液のｐＨを８
前後の弱アルカリ性に調布するための炭酸水素ナトリウ
ム等と先に混合して添加するかあるいは水溶液のｐＨを
弱アルカリ性に調節した後、安定ｆヒ削として単独で添
加する方法がある。また固型もしくは液体（でおいては
、グルタールアルデヒド水溶液のｐＨを弱アルカリ性に
調節した後に添加する方法がある。In the present invention, the stabilizing agent is added to an aqueous solution of glutaraldehyde in a powdered form, and the pH of the aqueous solution is adjusted to 8.
There is a method in which it is added by first mixing it with sodium hydrogen carbonate or the like to adjust the pH of the aqueous solution to be slightly alkaline, or it is added alone as a stable french after adjusting the pH of the aqueous solution to be slightly alkaline. Alternatively, there is a method in which the pH of the glutaraldehyde aqueous solution is adjusted to weak alkalinity before adding it in solid or liquid form.
本発明により調製されたｐＨ７，５〜９０の弱アルカリ
性グルタールアルデヒド水溶液中には、室温下３〜４週
間経過した後でも、濁りもしくは沈澱という現象は全く
認められなかった。この期間中濁りもしくは沈澱という
現象が水溶液中に認められなければ、グルタールアルデ
ヒド水溶液の殺菌効力の維持される期間を超えるため、
それ以後に沈澱等の認められる可能性があるとしても、
実用上において水溶液は殺菌消毒剤として使用不可であ
るだめ問題とはならない。In the slightly alkaline aqueous glutaraldehyde solution of pH 7.5 to 90 prepared according to the present invention, no phenomenon of turbidity or precipitation was observed even after 3 to 4 weeks at room temperature. If the phenomenon of turbidity or precipitation is not observed in the aqueous solution during this period, the period during which the bactericidal efficacy of the glutaraldehyde aqueous solution is maintained will be exceeded.
Even if there is a possibility that precipitation etc. may be observed after that,
In practical terms, this does not pose a problem since aqueous solutions cannot be used as sterilizing agents.
本発明を下記の実施例及び試験列により更に説明する。The invention is further illustrated by the following examples and test series.
下記実施例及び試験列（は単に本発明の説明のためであ
り、本発明をなんら限定するものではない。The following examples and test series are merely illustrative of the invention and are not intended to limit the invention in any way.
実施例１
２％濃度のグルタールアルデヒド水溶液１［］ Ｏｍｌ
に、炭酸水素ナトリウム６００肩り、亜硫酸ナトリウム
２００ｍｇを溶解した後、塩酸試液適量を滴下し水溶液
のｐＨを８２に調節する。Example 1 2% concentration glutaraldehyde aqueous solution 1 [] Oml
After dissolving 600 mg of sodium bicarbonate and 200 mg of sodium sulfite, an appropriate amount of hydrochloric acid test solution was added dropwise to adjust the pH of the aqueous solution to 82.
実施例２
２係濃度のグルタールアルデヒド水溶液１００ｍ？に、
炭酸水素す） ｌ）ラム３００ｍ９１メタ重亜硫酸ナト
リウム２００ｍ！７を溶解した後、水酸化ナトリウム試
液適量を滴下し水溶液のｐＨを８２に調節する。Example 2 100ml of glutaraldehyde aqueous solution with concentration 2? To,
Hydrogen carbonate) l) Rum 300m91 Sodium metabisulfite 200m! After dissolving 7, an appropriate amount of sodium hydroxide test solution was added dropwise to adjust the pH of the aqueous solution to 82.
実施ツ１１３
２％濃度のグルタールアルデヒド水溶液１００＋ｘＪに
、炭酸水素ナトリウム６００ｍｇ、グリ２フ２００ｍダ
を溶解した後、水酸化ナトリウム試Ｉ夜適量を滴下し水
溶液のｐＨを８２に調節する。Practice 113 After dissolving 600 mg of sodium hydrogen carbonate and 200 m of Glyph 2 in 100+xJ of a 2% concentration glutaraldehyde aqueous solution, the pH of the aqueous solution was adjusted to 82 by dropping an appropriate amount of sodium hydroxide test I.
実施例４
２％濃度のグルタールアルデヒド水溶液１００ｍ？に、
炭酸水素ナトリウム６００Ｍ！、 ピコガロール２００
労を溶解した後、水酸化ナトリウム試液適量を滴下し水
溶液のｐＨを８．２に調節する。Example 4 100ml of 2% concentration glutaraldehyde aqueous solution? To,
Sodium hydrogen carbonate 600M! , Picogalor 200
After dissolving the aqueous solution, add an appropriate amount of sodium hydroxide test solution dropwise to adjust the pH of the aqueous solution to 8.2.
試験例１
前記実施例１〜４と記、成された手順を使用して亜硫酸
ナトリウム、メタ重亜硫酸ナトリウム、グリシン、ピロ
ガロールの各安定化剤のそれぞれ２００７１Ｉｇを含有
する２％儂度のグルタールアルデヒド水溶液を試料溶液
として調、製した。Test Example 1 2% strength glutaraldehyde containing 20,071 Ig each of sodium sulfite, sodium metabisulfite, glycine, and pyrogallol stabilizers using the procedures described and completed in Examples 1 to 4 above. An aqueous solution was prepared as a sample solution.
該試料溶液１００ｍ１を容量１００＋ｘ？のガラス製ビ
ーカーに入れ、時計皿をビーカーにかぶせた後、４０℃
に設定した空気恒温槽に４日間放置した後、水溶液の濁
度を測定し、下記の式に従って得られる防止率を求めた
。100ml of the sample solution in a volume of 100+x? Place it in a glass beaker, cover the beaker with a watch glass, and heat to 40℃.
After being left in an air constant temperature bath set at 4 days, the turbidity of the aqueous solution was measured, and the prevention rate obtained was determined according to the following formula.
即ち、防止率（％）がＯもしくは負の直となる場合には
、安定化剤に防止能力の無いことを意味し、１００なら
ば、完全に水溶液の濁りを防止していることを意味する
。In other words, if the prevention rate (%) is O or a negative value, it means that the stabilizer has no prevention ability, and if it is 100, it means that it completely prevents the aqueous solution from becoming cloudy. .
濁度の計測１は水溶液の色彩変化が生じても測定に影響
を受けなくするため積分球式の濁度計を使用し行なった
。Turbidity measurement 1 was carried out using an integrating sphere type turbidity meter so that the measurement would not be affected by color changes in the aqueous solution.
対照試料として２％濃度のグルタールアルデヒド水溶液
１００ｍ１に、炭酸水素すトリウム３００ｍ９を溶解し
た後、水酸化ナトリウム試液適叶を１滴下し水溶液のｐ
Ｈを８２に調節したものを使用した。結果を表１に示す
。 “
表１
１：亜硫酸ナトリウム
２：メタ重亜硫酸ナトリウム
５ニゲリシン
４：ビロガロ〜ル
各安定剤とも全く濁度を認めなかった。As a control sample, dissolve 300ml of sodium bicarbonate in 100ml of a 2% concentration glutaraldehyde aqueous solution, then add one drop of sodium hydroxide test solution to adjust the pH of the aqueous solution.
The one whose H was adjusted to 82 was used. The results are shown in Table 1. Table 1 1: Sodium sulfite 2: Sodium metabisulfite 5 Nigericin 4: Virogalol No turbidity was observed at all with each stabilizer.
試験例２
メタ重亜硫酸ナトリウムのＩ走度を変化させて、２％グ
ルタールアルデヒド水溶、夜の濁度に対する安定化剤の
添加濃度の影響を求めた。１ｉｌｔ１定方法等は前記試
験しｌ １と同隊であったが試料溶液の放置期間につい
ては１週間とした結果を表２に示す。Test Example 2 The influence of the concentration of the stabilizer added on the night turbidity of 2% glutaraldehyde in water was determined by changing the I-travel of sodium metabisulfite. Table 2 shows the results of the 1ilt1 determination method, etc., which were the same as those tested above, but the sample solution was allowed to stand for 1 week.
表２
２％濃度のグルタールアルデヒド水溶液１０ｏｍｔに対
しメタ重亜硫酸ナトリウム０．４ ｐｐｔＭを添加する
ことにより９９．．６％の１濁り防止効果が得られ、そ
れ以上の添加計では、１００係の防止効果が認められた
。Table 2 By adding 0.4 pptM of sodium metabisulfite to 10 omt of a 2% concentration aqueous glutaraldehyde solution, 99. ．． A 1 turbidity prevention effect of 6% was obtained, and a 100 degree prevention effect was observed when adding more.
試験例ろ
前記実施例１〜４に記１伐の手順に従い亜硫酸ナトリウ
ム、メタ重亜硫酸ナトリウム、グリシン、ピロガロール
の各安定化剤をそれぞれ２チ儂度のグルタールアルデヒ
ド水溶液ｉ［］０．ｖ＋ｔＫ対し２００巧を含有する試
料溶液をそれぞれ５００ｍｔ調製した。Test Example: According to the procedure described in Examples 1 to 4 above, each of the stabilizers sodium sulfite, sodium metabisulfite, glycine, and pyrogallol was added to 20% of an aqueous solution of glutaraldehyde. 500 mt of each sample solution containing 200 mt for v+tK was prepared.
上記試料１＃ン夜のチフス菌、黄色ブドウ球菌、犬腸曹
、緑膿菌に対するフェノール係数を測定した試験に使用
した菌株は、チフス菌ｌ−ｌ９ＱｉＷ。The strain used in the test to measure the phenolic coefficient against Salmonella typhi, Staphylococcus aureus, S. aeruginosa, and Pseudomonas aeruginosa in Sample 1 was Salmonella typhi 1-19QiW.
黄色ブドウ球菌ＦＤＡ２０９Ｆ、大腸菌ＩＦＯ３８０６
、緑膿？１ｊｉＡＴＣＣ９０２７であった。Staphylococcus aureus FDA209F, Escherichia coli IFO3806
, aeruginosa? 1ji ATCC9027.
対照試料として前記試験例１に記載した手順に従い２％
濃度のグルタールアルデヒド＊Ｊ［５００ｙｘｌを調、
製した。結果を表６に示す。2% as a control sample according to the procedure described in Test Example 1 above.
Concentration of glutaraldehyde*J [adjust 500yxl,
Manufactured. The results are shown in Table 6.
亜硫酸ナトリウム、メタ重亜硫酸ナトリウム、ピロガロ
ールを安定化剤として用いた場合、対照試料と同等の殺
菌効力を有することが証明された。When sodium sulfite, sodium metabisulfite, and pyrogallol were used as stabilizers, they were proven to have the same bactericidal efficacy as the control sample.
表６
１：亜硫酸水素ナトリウム
２：メタ重亜硫酸ナトリウム
３：グリシン
４：ピロガロール
試験例４
前記実施例１〜４の手順に従い、亜硫酸ナトリウム、メ
タ重亜＠酸ナトリウム、グリシン、ピロガロールの各安
定化剤を有する２％濃度のグルタールアルデヒド水溶液
１ｐをそれぞれ調製し、容惜１βのガラス製ビーカーに
入れ、ビーカーの上にガラス板をかぶせた後室温下４週
間放置した。Table 6 1: Sodium bisulfite 2: Sodium metabisulfite 3: Glycine 4: Pyrogallol Test Example 4 Each stabilizer of sodium sulfite, sodium metabisulfite, glycine, and pyrogallol was prepared according to the procedures of Examples 1 to 4 above. 1 p of a 2% concentration aqueous glutaraldehyde solution having 2% concentration was prepared, placed in a glass beaker with a capacity of 1β, and after covering the beaker with a glass plate, it was left at room temperature for 4 weeks.
４週間後の水溶液の性状を目視にて観察した結果、色調
において若干の変１ヒを認めたものの、濁りもしくは沈
澱という現象に関しては、その生成を全く認めなかった
。As a result of visual observation of the properties of the aqueous solution after 4 weeks, although some changes in color tone were observed, no formation of turbidity or precipitation was observed.
（特許出願人 丸石製薬株式会社） （代理人 弁理士 １谷 安）(Patent applicant Maruishi Pharmaceutical Co., Ltd.) (Representative Patent Attorney Yasu Ichitani)
Claims (3)
反応する官能基を有する安定化剤を添加することを特徴
とする弱アルカリ性グルタールアルデヒド水溶液中に発
生する濁りもしくは沈澱現象に対する防止方法。(1) A method for preventing turbidity or precipitation occurring in a weakly alkaline glutaraldehyde aqueous solution, which comprises adding to the glutaraldehyde aqueous solution a stabilizer having a functional group that reacts with an aldehyde group.
トリウム、ロンガリット、ハイドロサルファイト、メタ
重亜硫酸ナトリウム、過硫酸ナトリウム、グリシン、ア
スパラギン酸、Ｌ−システイン塩酸塩、アスコルピン酸
、ポリビニルアルコール、フェロログルシノールより成
る群の内より選ばれた少くとも１種を用いる特許請求の
範囲第１項記載の沈澱防止方法。(2) As a stabilizer, sodium bisulfite, sodium sulfite, Rongalite, hydrosulfite, sodium metabisulfite, sodium persulfate, glycine, aspartic acid, L-cysteine hydrochloride, ascorbic acid, polyvinyl alcohol, ferrologlucinol The method for preventing precipitation according to claim 1, using at least one selected from the group consisting of:
液１００ｍｌ当り０．４ｍＭ以上を添加する特許請求の
範囲第１項記載の沈澱防止方法。(3) The method for preventing precipitation according to claim 1, wherein the stabilizer is added in an amount of 0.4 mM or more per 100 ml of a 2% concentration glutaraldehyde aqueous solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25531386A JPS63112532A (en) | 1986-10-27 | 1986-10-27 | Preventing method for clouding or precipitating of glutaraldehyde aqueous solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25531386A JPS63112532A (en) | 1986-10-27 | 1986-10-27 | Preventing method for clouding or precipitating of glutaraldehyde aqueous solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63112532A true JPS63112532A (en) | 1988-05-17 |
| JPH0529337B2 JPH0529337B2 (en) | 1993-04-30 |
Family
ID=17277042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP25531386A Granted JPS63112532A (en) | 1986-10-27 | 1986-10-27 | Preventing method for clouding or precipitating of glutaraldehyde aqueous solution |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63112532A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008070188A (en) * | 2006-09-13 | 2008-03-27 | Miura Co Ltd | Quantitative determination method of iron |
| US8252747B2 (en) | 2003-07-23 | 2012-08-28 | Johan Lowinger | Tissue adhesive sealant |
-
1986
- 1986-10-27 JP JP25531386A patent/JPS63112532A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8252747B2 (en) | 2003-07-23 | 2012-08-28 | Johan Lowinger | Tissue adhesive sealant |
| JP2008070188A (en) * | 2006-09-13 | 2008-03-27 | Miura Co Ltd | Quantitative determination method of iron |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0529337B2 (en) | 1993-04-30 |
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