JPS625339A - Interstitial liquid sampler of skin tissue - Google Patents
Interstitial liquid sampler of skin tissueInfo
- Publication number
- JPS625339A JPS625339A JP60145381A JP14538185A JPS625339A JP S625339 A JPS625339 A JP S625339A JP 60145381 A JP60145381 A JP 60145381A JP 14538185 A JP14538185 A JP 14538185A JP S625339 A JPS625339 A JP S625339A
- Authority
- JP
- Japan
- Prior art keywords
- interstitial fluid
- epidermis
- suction
- liquid reservoir
- suction hole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- Sampling And Sample Adjustment (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、皮膚表面を吸引することによって得られる間
質液を、効率よく取得して臨床検査の究極的目標とされ
ている無侵襲非観血測定用の試料として提供するための
表皮組織の間質液取得装置に関するものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention efficiently obtains interstitial fluid obtained by suctioning the skin surface, which is a non-invasive and non-invasive method that is the ultimate goal of clinical testing. The present invention relates to an apparatus for obtaining interstitial fluid of epidermal tissue to be provided as a sample for invasive measurement.
間質液は生体の皮膚表面を吸引する事によって得られる
液体であって、血液と同様に生体の医学的状態を検査す
るために必要な種々の成分を含有している。本発明の発
明者は、無侵襲非観血測定のための血液にとって代る測
定試料としてこの間質液に注目し、検討を進めて来た。Interstitial fluid is a liquid obtained by suctioning the skin surface of a living body, and like blood, it contains various components necessary for testing the medical condition of the living body. The inventor of the present invention has focused on this interstitial fluid as a measurement sample that can replace blood for non-invasive, non-invasive measurements, and has proceeded with studies.
しかし、−間質液は皮膚表面の単位面積当りの浸出量が
極めて微量であって測定試料として十分な量を取得する
ことは非常に困難であるという問題点があり、各種分析
のために十分な量の間質液を取得するには、皮膚表面の
広い面積から吸引する必要がある。ところが皮膚の広い
面積に亘って吸引操作を行うと、比較的低い吸引圧力で
あっても皮膚組織の変形が生ずるという問題点があり、
さらに間質液は皮膚の吸引面全体に分散して浸出するの
でこれを簗めるのが非常に困難であるという問題点があ
った。However, there is a problem in that the amount of interstitial fluid exuded per unit area of the skin surface is extremely small, making it extremely difficult to obtain a sufficient amount as a measurement sample. To obtain a sufficient amount of interstitial fluid, it is necessary to aspirate from a large area of the skin surface. However, when a suction operation is performed over a wide area of the skin, there is a problem in that deformation of the skin tissue occurs even at a relatively low suction pressure.
Furthermore, there is a problem in that interstitial fluid is dispersed and oozes out over the entire suction surface of the skin, making it extremely difficult to slough it off.
本発明は前記従来技術の問題点に鑑み、皮膚に組織変形
を生じさせることなく９種々の分析を行うのに十分な量
の間質液を難なく容易に取得することを目的とし２間質
液の取得対象となる表皮に上面を当接される環状部と、
該環状部の内側にあって前記表皮を支持する網状体と、
咳網状体を支承し該網状体の下方に吸引孔を有する支承
部と。In view of the problems of the prior art, the present invention aims to easily and easily obtain a sufficient amount of interstitial fluid to perform nine different analyzes without causing tissue deformation in the skin. an annular portion whose upper surface is brought into contact with the epidermis to be obtained;
a net-like body that is located inside the annular portion and supports the epidermis;
A support part that supports a cough reticular body and has a suction hole below the reticular body.
該吸引孔を通じて吸引する吸引手段とを具備することを
特徴とする表皮組織の間質液取得装置を提供せんとする
ものである。It is an object of the present invention to provide an interstitial fluid acquisition device for epidermal tissue, characterized by comprising a suction means for suctioning through the suction hole.
本発明によれば、環状部の上面を表皮の下面に気密に当
接させ、環状部の内側にあって支承部で支承されている
網状体で表皮を支持して網状体の下方にある吸引孔から
表皮が離れている状態を保持しつつ、吸引孔を通じて吸
引手段によって表皮組織を変形させることなく、当該表
皮の広い範囲から均等に吸引して、必要な量の間質液を
取得し得るものである。According to the present invention, the upper surface of the annular portion is brought into airtight contact with the lower surface of the epidermis, the epidermis is supported by the net-like body inside the annular portion and supported by the support portion, and the suction is provided below the net-like body. The required amount of interstitial fluid can be obtained by uniformly suctioning from a wide range of the epidermis through the suction hole without deforming the epidermal tissue by means of a suction means, while maintaining the epidermis separated from the hole. It is something.
本発明の実施例を図面に即して説明する。第１図及び第
２図に示すように、方形の六面体１の平坦な上面１ａに
９円形の環状部２を突設する。該環状部２は、上面が平
面２ａになっている。該環状部２の内側の前記六面体１
の上面１ａの上に該環状部２の前記平面２ａとほぼ面一
となる状態にてステンレスの網状体３を載置し、該六面
体１の上部を支承部とする。前記六面体１は、上面の前
記環状部２と同心的位置に間質液を吸引するための開口
部１ｂを有し、下面には真空ポンプ等の吸引装置に接続
される開口部１ｃを有する。前記六面体１の内部に液溜
め部（リザーバー）４を設け。Embodiments of the present invention will be described with reference to the drawings. As shown in FIGS. 1 and 2, nine circular annular portions 2 are provided protruding from a flat upper surface 1a of a rectangular hexahedron 1. As shown in FIGS. The annular portion 2 has a flat top surface 2a. The hexahedron 1 inside the annular part 2
A stainless mesh body 3 is placed on the upper surface 1a of the hexahedron 1 so as to be substantially flush with the flat surface 2a of the annular portion 2, and the upper portion of the hexahedron 1 is used as a support portion. The hexahedron 1 has an opening 1b for suctioning interstitial fluid at a position concentric with the annular portion 2 on the upper surface, and an opening 1c on the lower surface to be connected to a suction device such as a vacuum pump. A liquid reservoir 4 is provided inside the hexahedron 1.
該液溜め部４の上部と前記下面の開口部１ｃとを吸引孔
５によって連絡すると共に該吸引孔５の反対側であって
前記液溜め部４の適当な位置と１面の開口部１ｂとを吸
引孔６によって連絡する。The upper part of the liquid reservoir 4 and the opening 1c on the lower surface are connected by a suction hole 5, and the opening 1b on one surface is connected to an appropriate position of the liquid reservoir 4 on the opposite side of the suction hole 5. are communicated through the suction hole 6.
間質液の取得操作を行う場合は、取得対象となる表皮Ｓ
の下面に環状部２の平面２ａを気密に当接させ、＃￥！
状体３及び該網状体３を支承する六面体１の上面１ａで
表皮Ｓを支承しつつ、下面の開口部１ｃに接続した図示
しない真空ポンプ等の吸引装置によって吸引して表皮Ｓ
から間質液を取得する。このとき網状体３は表皮Ｓの変
形を防ぎ。When performing an interstitial fluid acquisition operation, the epidermis S to be acquired is
The flat surface 2a of the annular portion 2 is brought into airtight contact with the lower surface of the #¥!
While supporting the epidermis S on the upper surface 1a of the hexahedron 1 that supports the net-like body 3 and the net-like body 3, the epidermis S is suctioned by a suction device such as a vacuum pump (not shown) connected to the opening 1c on the lower surface.
Obtain interstitial fluid from At this time, the mesh body 3 prevents the epidermis S from deforming.
該表皮Ｓと六面体１の環状部２内の上面とは網状体３を
介して離れた状態が保持され、該表皮Ｓの全面から間質
液を取得することができる。取得された間質液は液溜め
部４の最下部に溜められ、該液溜め部４の存在によって
、吸引孔５への間質液の引き込みを防止することができ
る。液溜め部４に溜められた間質液は、取得操作が終了
した後。The epidermis S and the upper surface of the annular portion 2 of the hexahedron 1 are kept separated via the reticular body 3, and interstitial fluid can be obtained from the entire surface of the epidermis S. The obtained interstitial fluid is stored at the lowest part of the liquid reservoir 4, and the presence of the liquid reservoir 4 can prevent the interstitial fluid from being drawn into the suction hole 5. The interstitial fluid stored in the fluid reservoir 4 is collected after the acquisition operation is completed.
上面の開口部１ｂから適当な方法で吸引され試料として
利用される。It is aspirated by an appropriate method through the opening 1b on the top surface and used as a sample.
第３図及び第４図は本発明の別の実施例を示す。3 and 4 show another embodiment of the invention.
前記実施例における下面の開口部１ｃに代えて六面体１
の側面に開口部１ｃ’を設け、液溜め部４の最下部に連
通し、六面体１の下面に開口部１ｄを有する間質液の抽
出孔７を設けて構成する。第５図は取得された間質液を
利用して、検査結果の瞬時値を連続的に出力することが
できる場合の本発明の実施例を示すブロック図である。In place of the opening 1c on the lower surface in the above embodiment, a hexahedron 1 is used.
An opening 1c' is provided on the side surface of the hexahedron 1, communicating with the lowest part of the liquid reservoir 4, and an interstitial fluid extraction hole 7 having an opening 1d is provided on the lower surface of the hexahedron 1. FIG. 5 is a block diagram showing an embodiment of the present invention in which instantaneous test results can be continuously output using the acquired interstitial fluid.
表皮組織の間質液取得装置８を用いて、圧力計９によっ
て真空圧力を検知しつつ真空ポンプ１ｏで表皮Ｓを吸引
し、取得された間質液は液溜め部４の下部に落下し、抽
出孔７を通じて抽出され、微小バイオセンサＡ例えばグ
ルコース又は尿素の専用センサに導かれる。該微小バイ
オセンサＡで検知した検査情報は、電気信号として増幅
器Ｂに入力されて増幅された後、データプロセッサＣで
必要な血中濃度との相関補正等を行ってデータとして出
力し。Using the epidermal tissue interstitial fluid acquisition device 8, the epidermis S is sucked by the vacuum pump 1o while the vacuum pressure is detected by the pressure gauge 9, and the acquired interstitial fluid falls to the lower part of the liquid reservoir 4, It is extracted through the extraction hole 7 and guided to a microbiosensor A, such as a dedicated sensor for glucose or urea. The test information detected by the micro biosensor A is input as an electrical signal to an amplifier B and amplified, and then a data processor C performs necessary correlation correction with the blood concentration and outputs it as data.
表示・記録計りで表示・記録する。一方、測定済みの間
質液は液溜め已に導かれ、液体移送用の小型ポンプＦに
て移送され別の測定に再利用されるか又は廃棄される。Display and record using a scale. On the other hand, the interstitial fluid that has already been measured is led to a liquid reservoir, transferred by a small pump F for liquid transfer, and either reused for another measurement or discarded.
本発明に係る装置の前記実施例において重要な点は９間
質液を取得する対象となる表皮を、網状体３によって開
口部１ｂから離間させて均一に吸引すること１表皮組織
の変形を防ぐために環状体２の厚さが厚過ぎないように
留意すること、網状体３の目の粗さは表皮組織の変形を
防ぐために細かくする必要があるが、細か過ぎると間質
液が網目に付着して十分に取得できないので適当に選定
すること、吸引孔６はできるだけ小さく、かつ短かくし
て該吸引孔６の壁面に付着する間質液の量が少くなるよ
うにして間質液の取得量を確保することである。In the above embodiment of the device according to the present invention, the important points are 9 to uniformly suction the epidermis from which interstitial fluid is to be obtained by separating it from the opening 1b by the mesh body 3, and 1 to prevent deformation of the epidermal tissue. Care must be taken to ensure that the thickness of the annular body 2 is not too thick in order to protect the mesh, and the mesh of the mesh 3 needs to be fine to prevent deformation of the epidermal tissue, but if it is too fine, interstitial fluid may adhere to the mesh. Therefore, the amount of interstitial fluid obtained should be reduced by making the suction hole 6 as small and short as possible to reduce the amount of interstitial fluid that adheres to the wall of the suction hole 6. It is to ensure that
前記実施例において、方形の六面体１に環状部２と、支
承部と、液溜め部４と、吸引孔５．６とを設けて一体化
した構造を示したが、六面体１の代りに円柱体や角柱体
その他の形態とすることも可能であり、又、環状部２と
支承部とを一体化した受皿状に形成し、液溜め部４を別
体として設け。In the above embodiment, the annular part 2, the support part, the liquid reservoir part 4, and the suction hole 5.6 were provided in the rectangular hexahedron 1 to form an integrated structure. Alternatively, the annular portion 2 and the support portion may be formed into a saucer shape in which the annular portion 2 and the support portion are integrated, and the liquid reservoir portion 4 may be provided separately.
これら相互間をパイプ又はボースで接続してもよい。These may be connected to each other by a pipe or a bow.
本発明に係る装置でウサギを対象とした実験によれば１
表皮面積−２吸引時間１時間当り、 ０．０２〜０．０
５ ｍｉｌの間質液を取得することができた。実験によ
って得られた間質液と同一のウサギから得た血清とを比
較するために組成分析した結果を第５図に図表として示
した。この図表によれば、グルコースおよび非蛋白性窒
素の低分子量生体物質濃度は両者の間で良い一致を示し
、脂質及び蛋白質の高分子量生体物質濃度は間質液の方
が血清よりも低（、特に脂質については血清の数分の−
である。この脂質や蛋白質は、各種のバイオセンサに付
着してその寿命を縮めるので、自動血液生化学分析にお
ける大きな障害となっている。従って脂質や蛋白質の含
有量が少く、シかも必要なグルコースや非蛋白物質を血
液と同様に含んでいる間質液を、血液の代りに用いるこ
とによって、バイオセンサの寿命を延ばすことができ、
生化学分析の自動化を行う上で非常に有利である。According to experiments on rabbits using the device according to the present invention, 1
Epidermal area - 2 per hour of suction time, 0.02 to 0.0
It was possible to obtain 5 mil of interstitial fluid. The results of compositional analysis for comparison of the interstitial fluid obtained in the experiment and the serum obtained from the same rabbit are shown graphically in FIG. According to this diagram, the concentrations of low molecular weight biosubstances of glucose and non-protein nitrogen show good agreement between the two, while the concentrations of high molecular weight biosubstances of lipids and proteins are lower in interstitial fluid than in serum. In particular, regarding lipids, it is several times lower than that of serum.
It is. These lipids and proteins adhere to various biosensors and shorten their lifespans, posing a major hindrance to automated blood biochemical analysis. Therefore, by using interstitial fluid instead of blood, which has a low content of lipids and proteins and also contains necessary glucose and non-protein substances, the lifespan of the biosensor can be extended.
This is very advantageous in automating biochemical analysis.
本発明の前記実施例によれば、環状部と、支承部と、液
溜め部と、吸引孔とを一体化しているので装置が堅牢で
取扱いが容易であり、液溜め部の下部に抽出孔を設け、
該抽出孔から抽出した間質液を微小バイオセンサで検知
することにより検査結果を瞬時に連続的に入手できると
いう顕著な効果を奏する。According to the embodiment of the present invention, the annular portion, the support portion, the liquid reservoir, and the suction hole are integrated, so the device is robust and easy to handle, and the extraction hole is provided at the bottom of the liquid reservoir. established,
By detecting the interstitial fluid extracted from the extraction hole with a microbiosensor, a remarkable effect is achieved in that test results can be obtained instantaneously and continuously.
本発明によれば、環状部の上面を１間質液の取得対象と
なる表皮に当接させ、環状部の内側にあって支承部で支
承される網状体で表皮を支持し。According to the present invention, the upper surface of the annular part is brought into contact with the epidermis from which interstitial fluid is to be obtained, and the epidermis is supported by the reticular body located inside the annular part and supported by the support part.
網状体の下方に有る吸引孔を通じて吸引手段によって表
皮を吸引するので１表皮は網状体で支持された状態で網
目を通じて吸引され１表皮組織の変形がなり、網状体に
よって表皮が吸引孔から離れた状態を保持しつつ表皮の
広い範囲から均一に吸引して皮膚の吸引面全体に分散し
て侵出する間質流を取得することができ、更に吸引孔の
開口部と吸引手段との間に液溜め部（リザーバー）を介
在させれば、吸引手段によって間質液が引込まれて散失
することがなく、液溜め部に間質液を溜めて確実に取得
することができる。Since the epidermis is suctioned by the suction means through the suction holes located below the net, the epidermis is sucked through the mesh while being supported by the net, and the epidermis is deformed, causing the epidermis to separate from the suction holes by the net. It is possible to uniformly suction from a wide range of the epidermis while maintaining the same condition, and obtain an interstitial flow that is dispersed and infiltrated over the entire suction surface of the skin. If a liquid reservoir is provided, the interstitial fluid will not be drawn in and lost by the suction means, and the interstitial fluid can be stored in the liquid reservoir and reliably obtained.
第１図は本発明の実施例を示す縦断側面図。
第２図は本発明の実施例を示す平面図。
第３図は本発明の別の実施例を示す縦断側面図。
第４図は本発明の別の実施例を示す平面図。
第５図は本発明に係る装置を用いて取得された間質液を
利用して、検査結果の瞬時値を連続的に出力する場合の
実施例を示すブロック図。
第６図は血清と間質液の組成分析の結果を示す図表であ
る。
１・・・六面体。
１ａ・・・上面。
２・・・環状部。
２ａ・・・平面。
３・・・網状体。
４・：・液溜め部。
５．６・・・吸引孔。
７・・・抽出孔。
８・・・間質液取得装置。
９・・・圧力計。
１０・・・真空ポンプ。
特許 出願人 荒 井 恒 窓
間 上 富田端彦
同 上 菊 地 眞
第１図
ｊ
第２図
第３′図
第４図
第５′図
第６図FIG. 1 is a longitudinal sectional side view showing an embodiment of the present invention. FIG. 2 is a plan view showing an embodiment of the present invention. FIG. 3 is a longitudinal sectional side view showing another embodiment of the present invention. FIG. 4 is a plan view showing another embodiment of the present invention. FIG. 5 is a block diagram showing an embodiment in which instantaneous values of test results are continuously output using interstitial fluid obtained using the device according to the present invention. FIG. 6 is a chart showing the results of compositional analysis of serum and interstitial fluid. 1... Hexahedron. 1a...Top surface. 2...Annular part. 2a...plane. 3... Reticular body. 4:・Liquid reservoir. 5.6...Suction hole. 7...Extraction hole. 8...Interstitial fluid acquisition device. 9...Pressure gauge. 10...Vacuum pump. Patent Applicant: Hisashi Arai, Madoka Ma, Hako Tomita, Makoto Kikuchi, Figure 1, Figure 2, Figure 3', Figure 4, Figure 5', Figure 6
Claims (2)
環状部と、該環状部の内側にあって前記表皮を支持する
網状体と、該網状体を支承し該網状体の下方に吸引孔を
有する支承部と、該吸引孔を通じて吸引する吸引手段と
を具備することを特徴とする表皮組織の間質液取得装置
。(1) An annular part whose upper surface is in contact with the epidermis from which interstitial fluid is to be obtained; a reticular body that is inside the annular part and supports the epidermis; 1. An interstitial fluid acquisition device for epidermal tissue, comprising: a support portion having a suction hole below; and a suction means for suctioning through the suction hole.
（リザーバー）を介在させた特許請求の範囲第１項記載
の表皮組織の間質液取得装置。(2) The interstitial fluid acquisition device for epidermal tissue according to claim 1, wherein a fluid reservoir is interposed between the opening of the suction hole and the suction means.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60145381A JPS625339A (en) | 1985-07-01 | 1985-07-01 | Interstitial liquid sampler of skin tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60145381A JPS625339A (en) | 1985-07-01 | 1985-07-01 | Interstitial liquid sampler of skin tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS625339A true JPS625339A (en) | 1987-01-12 |
Family
ID=15383926
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60145381A Pending JPS625339A (en) | 1985-07-01 | 1985-07-01 | Interstitial liquid sampler of skin tissue |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS625339A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01112442U (en) * | 1988-01-22 | 1989-07-28 | ||
| JPH0231740A (en) * | 1988-07-22 | 1990-02-01 | Nec Corp | Apparatus for measuring blood sugar level |
| JPH02286132A (en) * | 1989-04-28 | 1990-11-26 | Nec Corp | Instrument and method for measuring blood component |
| JPH06772U (en) * | 1992-06-17 | 1994-01-11 | 久留米運送株式会社 | Album with storage for developed photographic film |
-
1985
- 1985-07-01 JP JP60145381A patent/JPS625339A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01112442U (en) * | 1988-01-22 | 1989-07-28 | ||
| JPH0231740A (en) * | 1988-07-22 | 1990-02-01 | Nec Corp | Apparatus for measuring blood sugar level |
| JPH02286132A (en) * | 1989-04-28 | 1990-11-26 | Nec Corp | Instrument and method for measuring blood component |
| JPH06772U (en) * | 1992-06-17 | 1994-01-11 | 久留米運送株式会社 | Album with storage for developed photographic film |
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