JPS62262985A - Fermentation tank - Google Patents
Fermentation tankInfo
- Publication number
- JPS62262985A JPS62262985A JP10393486A JP10393486A JPS62262985A JP S62262985 A JPS62262985 A JP S62262985A JP 10393486 A JP10393486 A JP 10393486A JP 10393486 A JP10393486 A JP 10393486A JP S62262985 A JPS62262985 A JP S62262985A
- Authority
- JP
- Japan
- Prior art keywords
- foam
- sensor
- fermentation
- liquid
- defoaming
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 38
- 230000004151 fermentation Effects 0.000 title claims abstract description 36
- 239000006260 foam Substances 0.000 claims abstract description 35
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 239000002518 antifoaming agent Substances 0.000 claims abstract description 12
- 230000003254 anti-foaming effect Effects 0.000 claims description 5
- 239000007788 liquid Substances 0.000 abstract description 30
- 238000002347 injection Methods 0.000 abstract description 13
- 239000007924 injection Substances 0.000 abstract description 13
- 238000003756 stirring Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 3
- 238000012262 fermentative production Methods 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000007423 decrease Effects 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005187 foaming Methods 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 238000005273 aeration Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
Landscapes
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、発酵槽に関し、特に発酵工業の微生物培養槽
で用いられる消泡装置に関するもので、発酵槽に限らず
発泡を伴なう液の攪拌槽としても適用し得るものである
。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to fermenters, and in particular to defoaming devices used in microbial culture tanks in the fermentation industry. It can also be used as a stirring tank.
従来の発酵槽を第３図に示す。 A conventional fermenter is shown in Figure 3.
第３図において、発酵槽１の内部には散気管２、撹拌翼
５、泡切Ｒ７、電極式のほか超音波式等の泡感知固定セ
ンサ１０ｔ）が具備されている。発酵槽１に満たされた
発酵夜已に酸素を供給するため、空気が空気管Ｓを介し
て散気管２より発酵液ａに供給され、その中の酸素が溶
解される。酸素の溶解を助けるためと発酵液ａ中の菌体
が十分に混合されるように攪拌翼５が攪拌軸６を介して
回転する。発酵夜ａから泡沫すが発生し、泡感知センサ
１０ｂに接触すると、変換器１４と泡感知センサ１０１
）の電気回路に流れていた電流が増幅され、コントロー
ル弁４ａを作動させて開け、消泡液注入管４を介して消
泡液が槽１内に添加される。泡沫すが消滅すると泡感知
センサ１ｏ１）と変換器１４の間の電気回路に流れてい
た電流が微弱となυ、コントロール弁４ａは閉じる。In FIG. 3, the inside of the fermenter 1 is equipped with an aeration pipe 2, a stirring blade 5, a bubble cutter R7, and a fixed bubble detection sensor 10t, which is of an electrode type or an ultrasonic type. In order to supply oxygen to the fermentation liquid filled in the fermentation tank 1, air is supplied to the fermentation liquid a from the aeration pipe 2 via the air pipe S, and the oxygen therein is dissolved. A stirring blade 5 is rotated via a stirring shaft 6 in order to help dissolve oxygen and to sufficiently mix the bacterial cells in the fermentation liquid a. When foam is generated from fermentation night a and contacts the foam detection sensor 10b, the converter 14 and the foam detection sensor 101
) is amplified, the control valve 4a is actuated and opened, and the antifoaming liquid is added into the tank 1 via the antifoaming liquid injection pipe 4. When the foam disappears, the current flowing in the electric circuit between the foam detection sensor 1o1) and the converter 14 becomes weak υ, and the control valve 4a closes.
コントロール弁４ａは開信号が流れている間は、通常コ
ントロール周期を１０〜２０秒、１パルスあたりの開時
間を１〜２秒としている。While the open signal is flowing, the control valve 4a normally has a control period of 10 to 20 seconds and an opening time of 1 to 2 seconds per pulse.
閉信号となれば、直ちにコントロール弁４ａは閉じる。When the close signal is received, the control valve 4a is immediately closed.
この消泡剤注入態様は第４図に示す通りである。This antifoaming agent injection mode is as shown in FIG.
（１）泡沫に付着した菌体の一部は泡切翼７によって発
酵［ａの中に落下するが、大部分は発酵槽内壁面に付着
し、そこで増殖する（カビの場合は特にｗａｌｌ ｇｒ
ｏｗｔｈ ）現象を呈し、やがて塊状のスライムとなる
。(1) Some of the bacterial cells attached to the foam fall into the fermenter [a] by the foam cutting blade 7, but most of them adhere to the inner wall surface of the fermenter and multiply there (especially in the case of mold)
owth ) phenomenon, and eventually becomes a lumpy slime.
（２）発生した塊状スライムは発酵終了後菌体の回収の
際大きな労力を要し、場合によっては薬剤によらなけれ
ば除去できない。(2) The generated lumpy slime requires a great deal of effort when collecting the bacterial cells after fermentation, and in some cases, it cannot be removed without the use of chemicals.
（３）塊状スライムが増加すると酸素が十分に浸透しな
い部分は菌が死滅し発酵生産に寄与する菌体が減少し、
収率の低下を招く。(3) When the amount of lumpy slime increases, bacteria die in areas where oxygen does not penetrate sufficiently, and the number of bacteria that contribute to fermentation production decreases.
This results in a decrease in yield.
（４）流加培養〔発酵中、ある特定の基質（微生物に対
する栄養物質）を発酵槽へ供給するが、目的生成物（菌
体または菌体外生成物）は発酵終了時まで抜き取らない
ような培養操作をいう〕をおこなう場合、当初槽内の発
酵液量は少なく液面は低いが、発酵経過に伴ない液量が
増えて液面が上昇する。その間、発生した泡沫は上記（
１）のような現象を呈するが、発生するスライム量は回
分培養又は連続培養のような発酵槽内液面が一定のとき
に比べて著しく多い。即ち、泡感知固定センサ１０１）
と発酵ｌａの液面の間の距離が、流加培養のときに一時
的（通常で８時間以上）ではあるが、最も大きいからで
ある。(4) Fed-batch culture [during fermentation, a specific substrate (nutritional substance for microorganisms) is supplied to the fermenter, but the target product (bacterial cells or extracellular products) is not removed until the end of fermentation. When carrying out the culture operation], initially the amount of fermented liquid in the tank is small and the liquid level is low, but as the fermentation progresses, the amount of liquid increases and the liquid level rises. During that time, the foam generated is as shown above (
Although the phenomenon described in 1) occurs, the amount of slime generated is significantly larger than when the liquid level in the fermenter is constant, such as in batch culture or continuous culture. That is, bubble sensing fixed sensor 101)
This is because the distance between the liquid level of the fermentation la and the liquid level of the fermentation la is the largest during fed-batch culture, although it is temporary (usually 8 hours or more).
さらに悪いことに、一度壁面に付着したスライムが少し
でも発酵槽内の空気で乾燥すれば、その後、液面の上昇
に伴なって発酵液ａでスライムを再度濡らしても強く掻
き取らない限シ、付着スライムはほとんど脱離しない。Even worse, if the slime that has adhered to the walls dries even slightly in the air inside the fermenter, even if the slime is re-wetted with fermentation liquid a as the liquid level rises, the slime will remain intact unless it is strongly scraped off. , the attached slime hardly detaches.
本発明は、上記問題点を、
（１）発酵槽内壁面に泡沫が付着しないように、初期の
うちに消泡させること、
（２）上記方法を具現化するために、泡検知センサを適
性な位置に自在にセットし、消泡剤を注入させること、
それによシ泡沫の発生量を極力少なくすること、
によシ解決するものである。The present invention solves the above problems by (1) defoaming at an early stage to prevent foam from adhering to the inner wall surface of the fermenter; (2) in order to embody the above method, the foam detection sensor is appropriately configured. to freely set it in the desired position and inject antifoaming agent,
In addition, the problem can be solved by minimizing the amount of foam generated.
すなわち本発明は、消泡装置を備える発酵槽において、
発酵槽内に配置される泡感知部分が槽の高さ方向に自在
に移動が可能な手段を有する泡検出装置、およびその信
号によシ消泡剤を注入する装置を具備することを特徴と
する発酵槽に関する。That is, the present invention provides a fermenter equipped with an antifoaming device,
The fermentation tank is characterized by comprising a foam detection device in which a foam sensing portion disposed in the fermentation tank has a means that can freely move in the height direction of the tank, and a device for injecting an antifoaming agent based on the signal from the foam detection device. related to fermenters.
本発明発酵槽においては、発酵液ａの液面より発生する
泡沫が著しく大量にならないうちに、泡検出装置および
消泡剤注入装置が作動し、消泡する。それによって、槽
内壁面に泡沫が付着する量を低減する。In the fermentation tank of the present invention, the foam detection device and the antifoaming agent injection device are activated and defoaming occurs before the foam generated from the surface of the fermented liquid a becomes extremely large. This reduces the amount of foam adhering to the inner wall surface of the tank.
１、 構成 第１図は、本発明発酵槽の構成例を示す図である。 1. Configuration FIG. 1 is a diagram showing an example of the configuration of a fermenter according to the present invention.
第１図において、発酵槽１、散気管２、空気管５、消泡
液注入管４、攪拌翼５、攪拌軸６、泡切翼７、変換器１
４、コントロール弁４ａは、第５図と同じである。In FIG. 1, a fermenter 1, an aeration pipe 2, an air pipe 5, an antifoaming liquid injection pipe 4, a stirring blade 5, a stirring shaft 6, a foam cutting blade 7, a converter 1
4. The control valve 4a is the same as shown in FIG.
泡感知駆動セ／す１０ａはメカニカルシール１３を介し
てセンサ駆動シリンダ１１と連結しており、駆動フント
ロール装［１２によって上下方向に任意に移動できるよ
うになっている。シリンダ１１は油圧式の他、電動式等
が使用できる。コントロール弁４ａは開信号が流れてい
る間は、通常コントロール周期を２〜４秒、１パルスあ
たシの開時間をα１〜１２秒とする。閉信号となればフ
ン）Ｏ−ル弁４ａは直ちに閉じる。The foam sensing drive unit 10a is connected to the sensor drive cylinder 11 via a mechanical seal 13, and can be moved vertically as desired by a drive handle 12. The cylinder 11 can be of a hydraulic type or an electric type. While the open signal is flowing, the control valve 4a normally has a control period of 2 to 4 seconds and an opening time of 1 pulse of α1 to 12 seconds. When the close signal is received, the O-le valve 4a is immediately closed.
２ 作用
（１）泡沫すの発生量は、培養法、発酵液組成、発酵経
過時間、培養菌種等にもとづく発泡性により異なる。特
に、発酵経過時間についてみれば、菌体増殖時期に泡沫
すは最も多く、ついで発酵終了期に多く、発酵開始期が
最も少々いので、それに応じてセンサ１０ａの位置を変
える必要がある。回分、連続培養の場合、発酵液ａの液
面はほぼ一定しており、泡感知駆動センサ１０ａは発酵
経過時間によって駆動フントロール装置１２とシリンダ
１１を介して下げ、発酵液ａの液面との距離を短くする
。これによって、泡沫すとセンサ１０ａの接触頻度が増
えるが、泡沫すの量が少なくなるとともに、１パルスあ
たシの消泡剤の注入時間は短くて、注入量も少なくなる
。2. Effect (1) The amount of foam generated varies depending on the foaming property based on the culture method, fermentation solution composition, elapsed fermentation time, cultured bacterial species, etc. In particular, regarding the elapsed fermentation time, foaming is most frequent during the bacterial growth period, followed by the end of fermentation, and least at the beginning of fermentation, so it is necessary to change the position of the sensor 10a accordingly. In the case of batch or continuous culture, the liquid level of the fermented liquid a is almost constant, and the bubble detection drive sensor 10a lowers the liquid level of the fermented liquid a through the driving mount roll device 12 and the cylinder 11 depending on the elapsed fermentation time. shorten the distance. As a result, when foaming occurs, the frequency of contact with the sensor 10a increases, but the amount of foaming decreases, and the injection time of the antifoaming agent per pulse is short, resulting in a small amount of injection.
（２）流加培養の場合、発酵液器の液面は発酵時間の経
過に伴って、上昇する。泡感知駆動センナ１０ａは、発
酵液ａの液面との距離を一定に保つように、発酵時間の
経過とともに駆動フントロール装置１２とシリンダ１１
を介して徐々に上昇させる。これによって泡沫）とセン
サ１０ａの接触頻度は発酵経過時間に伴なって増えるが
、泡沫すの量が少なくなるとともに、１パルスあたりの
消泡剤の注入時間は短くて、注入量も少なくなる。(2) In the case of fed-batch culture, the liquid level in the fermentation liquid vessel rises as the fermentation time progresses. The foam sensing drive sensor 10a rotates the drive mount roll device 12 and the cylinder 11 as the fermentation time progresses so as to maintain a constant distance from the liquid level of the fermented liquid a.
Gradually rise through. As a result, the frequency of contact between the foam (foam) and the sensor 10a increases with the elapsed fermentation time, but the amount of foam decreases, the injection time of the antifoam agent per pulse is short, and the injection amount also decreases.
（３）以上の消泡剤の注入態様を第２図に示す。(3) The injection mode of the above antifoaming agent is shown in FIG.
本発明によれば、次のような効果を奏することができる
。According to the present invention, the following effects can be achieved.
（１）全発酵時間での消泡剤の注入全量は従来法とほぼ
同量であるが、泡沫すの発生量は少なく、さらに槽１の
壁面に付着する菌体量を著しく減らすことができる。(1) Although the total amount of antifoam agent injected during the entire fermentation time is approximately the same as in the conventional method, the amount of foam generated is small, and the amount of bacterial cells adhering to the wall of tank 1 can be significantly reduced. .
（２）それによって発酵生産の収率の低下を防ぐことが
できる。(2) This can prevent a decrease in the yield of fermentation production.
（３）同一槽において、回分、連続および流加培養のい
ずれでも実施することができ、従来においては実現でき
なかった多様な運転方法が可能となる。(3) Batch, continuous, and fed-batch culture can be carried out in the same tank, making it possible to perform a variety of operating methods that were not possible in the past.
第１図は本発明発酵槽の一実施例と示す図、第２図は本
発明発酵槽における消泡剤注入態様を模式的に示す図、
第３図は従来の発酵槽を示す図、第４図は従来の発酵槽
における消泡剤注入態様を模式的に示す図である。
１−発酵槽、２−散気管、３・・・・空気管、４・・−
消泡液注入管、４ｅ１／−・・コントロール弁、５−・
・攪拌翼、６−攪拌軸、７−・・泡切翼、８−駆動装置
、９−排気管、１０ａ−泡感知駆動センサ、１０ ｂ−
−−泡感知固定センサ、１１−センサ駆動シリンタ、１
２−ＩＫ動コントロール装置、１５−・・メカニカルシ
ール、１４−・変換器、ａ−・・発酵液、ｂ−・・泡沫
復代理人 内 １） 明
復代理人 萩 原 亮 −
復代理人 安 西 篤 夫
第３図FIG. 1 is a diagram showing an embodiment of the fermenter of the present invention, and FIG. 2 is a diagram schematically showing an antifoaming agent injection mode in the fermenter of the present invention.
FIG. 3 is a diagram showing a conventional fermenter, and FIG. 4 is a diagram schematically showing an antifoaming agent injection mode in the conventional fermenter. 1-Fermentation tank, 2-Diffuser pipe, 3... Air pipe, 4...-
Defoaming liquid injection pipe, 4e1/-...control valve, 5-...
- Stirring blade, 6-stirring shaft, 7--bubble cutting blade, 8-drive device, 9-exhaust pipe, 10a-foam detection drive sensor, 10b-
--Bubble sensing fixed sensor, 11-sensor driving cylinder, 1
2-IK motion control device, 15--mechanical seal, 14--converter, a--fermentation liquid, b--foam recovery agent 1) Meifuku agent Ryo Hagiwara - sub-agent Yasu Atsuo Nishi Figure 3
Claims (1)
る泡感知部分が槽の高さ方向に自在に移動が可能な手段
を有する泡検出装置、およびその信号により消泡剤を注
入する装置を具備することを特徴とする発酵槽。In a fermenter equipped with an antifoaming device, a foam detection device having a means for a foam sensing portion disposed in the fermentation tank to be freely movable in the height direction of the tank, and a device for injecting an antifoaming agent based on a signal from the foam detection device. A fermenter characterized by comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10393486A JPS62262985A (en) | 1986-05-08 | 1986-05-08 | Fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10393486A JPS62262985A (en) | 1986-05-08 | 1986-05-08 | Fermentation tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62262985A true JPS62262985A (en) | 1987-11-16 |
Family
ID=14367265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10393486A Pending JPS62262985A (en) | 1986-05-08 | 1986-05-08 | Fermentation tank |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62262985A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2684109A1 (en) * | 1991-11-22 | 1993-05-28 | Ajinomoto Kk | Culture device and process for aerobic fermentation with defoaming |
| JP2011147892A (en) * | 2010-01-22 | 2011-08-04 | Metawater Co Ltd | Method of methane fermentation |
| WO2022234836A1 (en) * | 2021-05-06 | 2022-11-10 | 味の素株式会社 | Fermentation control device and method |
-
1986
- 1986-05-08 JP JP10393486A patent/JPS62262985A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2684109A1 (en) * | 1991-11-22 | 1993-05-28 | Ajinomoto Kk | Culture device and process for aerobic fermentation with defoaming |
| JP2011147892A (en) * | 2010-01-22 | 2011-08-04 | Metawater Co Ltd | Method of methane fermentation |
| WO2022234836A1 (en) * | 2021-05-06 | 2022-11-10 | 味の素株式会社 | Fermentation control device and method |
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