JPS6183952A - Carrierless continuous electro-phoresis and apparatus therefor - Google Patents
Carrierless continuous electro-phoresis and apparatus thereforInfo
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- JPS6183952A JPS6183952A JP59204002A JP20400284A JPS6183952A JP S6183952 A JPS6183952 A JP S6183952A JP 59204002 A JP59204002 A JP 59204002A JP 20400284 A JP20400284 A JP 20400284A JP S6183952 A JPS6183952 A JP S6183952A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
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Abstract
Description
【発明の詳細な説明】
〔発明の利用分野〕
本発明は、たんばく質、核酸、ｍ泡等の荷電物質の電気
泳動法Ｉこよる分離精製技術（こ係わり、特に高分離性
能を有し、かつ、大量処理が可能な無担体電気泳動方法
および装置に関するものである。[Detailed Description of the Invention] [Field of Application of the Invention] The present invention relates to separation and purification technology using electrophoresis method I for charged substances such as proteins, nucleic acids, and m-bubbles. The present invention also relates to a carrier-free electrophoresis method and apparatus that are capable of large-scale processing.
従来のたんばく質、核酸、絹鳴等の荷電物質（以下、°
代表してたんばく質と呼ぶ）の分離精製法として、電気
泳動法、膜分離法、液体クロマトグラフィ等がある。膜
分離法は、ｌｉ−の孔の大きさによりたんばく質を分離
する方法で、連続処理ができるが、たんばく質の分離能
が劣るという欠点があり、液体クロマトグラフィは、た
んばく質を担体充填カラム中を通して分離する方法で、
分離能はすぐれているが、バッチ操作のため、工業規模
の大量処理には不適当である。また、電気泳動法は、た
んぽ（質の荷電量の差を゛利用して、電場中にて分離精
製する方法である。この電気泳動法には、ゲル等の担体
を用いる担体電気泳動法と、担体を用いず自由流動液中
にて行なう無担体電気泳動法がある。担体電気泳動法は
バッチ式であり、大量処理を行なうような工業化には、
連続処理ができる無担体電気泳法が適している。Conventional charged substances such as proteins, nucleic acids, and silk (hereinafter referred to as °
Examples of separation and purification methods for proteins (typically called proteins) include electrophoresis, membrane separation, and liquid chromatography. Membrane separation is a method that separates proteins based on the pore size of the li-, and allows for continuous processing, but it has the disadvantage of poor protein separation ability.Liquid chromatography separates proteins by using a carrier A method of separation through a packed column,
Although it has excellent separation ability, it is a batch operation, making it unsuitable for large-scale processing on an industrial scale. In addition, electrophoresis is a method of separation and purification in an electric field by utilizing the difference in the amount of charge between particles. There is a carrier-free electrophoresis method that is carried out in a free-flowing liquid without using a carrier.The carrier electrophoresis method is a batch method, and for industrialization that involves mass processing,
A carrier-free electrophoresis method that allows continuous processing is suitable.
無担体電気泳動法については、Ｂｌｅｃｔｒ。For carrier-free electrophoresis, Blectr.
ｐｈｏｒｅｓｉｓ １９８２，３．．２３５−２４３
におけるＫｕｒｔ Ｈａｎｎｌｇによる＠Ｎｅｗａｓ
ｐｅｃｔｓ ｉｎ ｐｒｅｐａｒａｔｉｖｅ ａ
ｎｄ ａｎａｌｙｔｉｃａｌ ｃｏｎｔ［ｎｕｏｕ
ｓ ｆｒｅｅ−ｆｌｏｖｒ ｃｅｌｌ ｅＩｅｃ
ｔｒｏｐｈｏｒｅｓｌｓ’″と題する文献において論じ
られている。また、この原理に基いた装置が、***Ｈｉ
ｒ ｓ ｈ ｍ ａ ｎ ｎ社ですでに製造されてい
る。phoresis 1982, 3. ．． 235-243
@Newas by Kurt Hannlg in
pects in preparative a
nd analytical cont[nuou
s free-flovr cell eIec
trophoresls'''. A device based on this principle was also developed in West Germany
It is already manufactured by R.S.H.M.A.N.
この無担体電気泳動法によるたんばく質の分離精製方法
について説明する。分離角チャンバ内で電場を横切りて
一定速度で流下する分離バッファー液中に、注入口から
連続的に分離すべきたんばく質の混合物を注入すと、各
たんばく質はそれぞれ荷電量が異なるため、電場中での
移動速度が異なり、そのため、分離バッファー液中を流
下中に、分離バッファー液流速との兼合いで、それぞれ
に偏向されて分離される。このように、本方法は連続的
に分離することができるため、工業規模のたんばく質の
分離精製に対して有効である。A method for separating and purifying proteins using this carrier-free electrophoresis method will be explained. When a mixture of proteins to be separated is continuously injected from an injection port into a separation buffer solution flowing at a constant speed across an electric field in a separation angle chamber, each protein has a different amount of charge. Their movement speeds in the electric field are different, and therefore, while flowing through the separation buffer solution, they are deflected and separated depending on the flow rate of the separation buffer solution. As described above, since the present method can perform continuous separation, it is effective for industrial-scale protein separation and purification.
本方法で分離性能を高めるためには、分離用チャンバ内
の分離バッファー液の流速を常に一定に保つことが重要
であるが、分離バッファー液には電流を流すため、ジュ
ール熱が必ず発生し、この熱により分離バッファー液に
対流現象を生じ、分離バッファー液の流れが乱れるため
、たんぽ（質の分離性能が低下する。この問題を解決す
るため、従来の無担体電気泳動法では、分離バッファー
液の温度や流速を±０．２％と極めて高精度にコントロ
ールし、また、装置を小形化してジュール熱に対応させ
ているが、たんぼ（質の分離性能はあまりよくなってい
ない。In order to improve the separation performance with this method, it is important to keep the flow rate of the separation buffer solution in the separation chamber constant at all times, but since a current is passed through the separation buffer solution, Joule heat is always generated. This heat causes a convection phenomenon in the separation buffer solution, which disrupts the flow of the separation buffer solution, resulting in a decrease in separation performance.To solve this problem, in conventional carrier-free electrophoresis, the separation buffer solution is Although the temperature and flow rate of the rice grains have been controlled with extremely high precision of ±0.2%, and the equipment has been miniaturized to handle Joule heat, the separation performance for rice fields has not improved much.
さらにまた、この熱対流の影響をなくするため、電気泳
動装置を宇宙空間に持って行き、無重力状態下で電気泳
動を行なわせる計画もあり、この対流がいかに大きな問
題かがうかがえる。Furthermore, in order to eliminate the effects of this thermal convection, there are plans to take an electrophoresis device into space and perform electrophoresis in zero gravity, which shows how big a problem this convection is.
本発明の目的は、高分離性能を有し、かつ、大量処理が
可能な無担体連続電気泳動方法および装置を提供するこ
とにある。An object of the present invention is to provide a carrier-free continuous electrophoresis method and apparatus that have high separation performance and are capable of mass processing.
未発明は、従来の無担体電気泳動装置における問題点が
、泳動室内で発生するジュール熱によるバッファ液の対
流混合である点に着目し、このジュール熱によるバッフ
ァ液の対流を利用して荷電物質を分離し、かつ、分離濃
縮した荷電物質をすべて重力方向に取出すようにしたも
ので、泳動室内で発生するジュール熱によるバッファ液
の泳動室中央部で上昇し両端部で下降する循環流に被処
理液を供給して荷電物質を分離し、処理液を泳動室２端
下部より取出すようにしたものである。The uninvented invention focused on the fact that a problem with conventional carrier-free electrophoresis devices was the convective mixing of buffer solutions due to Joule heat generated in the electrophoresis chamber. The separated and concentrated charged substances are all taken out in the direction of gravity, and are exposed to the circulating flow of buffer solution that rises in the center of the migration chamber and descends at both ends due to Joule heat generated in the migration chamber. A processing liquid is supplied to separate charged substances, and the processing liquid is taken out from the bottom of the two ends of the migration chamber.
以下、本発明の一実施例を第１図、第２図により説明す
る。第１図、第２図において、１は垂直に設置された泳
動装置本体、２は泳動装置本体１内中央部Ｉζ設けられ
た泳動室、３ａ、３ｂは泳動装置本体１内の泳動室２両
端に形成された電極室で、泳動室２とは半透膜４で仕切
られている。５ａ、５ｂは電極室３ａ、３ｂ内に設けら
れた白金線等の荷電用電極、６は泳動室２のほぼ中央部
側面に設けられた荷電物質を含む被処理液の入口ノズル
、７ａ、７ｂは泳動室２の両端下部１こ設けられた泳動
分離した処理液の出口ノズル、８ａ、８ｂおよび９ａ、
９ｂは電極室３ａ、３ｂの下端および上端にそれぞれ設
けられた電極液の入口ノズルおよび出口ノズルである。An embodiment of the present invention will be described below with reference to FIGS. 1 and 2. In FIGS. 1 and 2, 1 is a vertically installed electrophoresis device main body, 2 is a migration chamber provided in the central part Iζ of the electrophoresis device main body 1, and 3a and 3b are both ends of the migration chamber 2 inside the migration device main body 1. The electrophoresis chamber 2 is separated from the electrophoresis chamber 2 by a semipermeable membrane 4. 5a and 5b are charging electrodes such as platinum wires provided in the electrode chambers 3a and 3b; 6 is an inlet nozzle for a liquid to be processed containing a charged substance provided on the side surface of the approximately central portion of the migration chamber 2; 7a and 7b; 8a, 8b and 9a are outlet nozzles for the electrophoretically separated treatment liquid provided at the bottom of both ends of the electrophoresis chamber 2;
Reference numerals 9b denote an inlet nozzle and an outlet nozzle for the electrode solution provided at the lower and upper ends of the electrode chambers 3a and 3b, respectively.
上述した構成１ζおいて、泳動室２内Ｉζ泳動用のバッ
ファ液を張込み、電極室３ａ、３ｂ内化もバッフ１液を
外部より流入循環させて、荷電用電極５ａ、５ｂに所定
電圧をかけると、荷電電流によりジニール熱が発生し、
第１図に示すよう化泳動室２内のバッファ液に対流が生
じる。しかして、ジュール熱は泳動室２内で一様Ｉこ発
生するが、泳動室２の中央部では放熱し難いため、泳動
室２内中央部と両端部のバッファ液１こ温度差を生じ、
最も高温となる泳動室２内中央部のバッフ１液は上昇流
となり、泳動室２内両端部のバッファ液は下降流となっ
て泳動室２内を循環する。このようなバッファ液の循環
流れのある泳動室２内に、入口ノズル６よりたん白質な
どの電荷を持つ被処理液を供給すると、荷電物質の帯電
の正、負に応じて泳動室２内を対流により流動しながら
、負又は正の電極室３ｂ又は３ａの方向に移動する。こ
の場合、荷電用電極５ａを正、５ｂを負として直流電圧
をかけると、正に帯電した荷電物質は電極室３ｂ側に移
動・して、泳動室２内の電極室３ｂ側下部にＷｋｍされ
、処理液として出口ノズル７ｂより取出される。同様に
負薯こ帯電した荷電物質は電極室３ａ側に移動して、泳
動室２内の電極室３ａ側下部に濃縮され、処理液として
出口ノズル７ａより取出される。この結果、複数の荷電
物質の帯電状態、すなわち、帯電の正又は負に応じて連
続的に分離することができる。In the configuration 1ζ described above, a buffer solution for Iζ migration is filled in the migration chamber 2, and a buffer 1 solution is introduced from the outside and circulated in the electrode chambers 3a and 3b, and a predetermined voltage is applied to the charging electrodes 5a and 5b. When applied, the charged current generates ginyl heat,
Convection occurs in the buffer solution in the electrophoresis chamber 2 as shown in FIG. Therefore, although Joule heat is generated uniformly within the migration chamber 2, it is difficult to dissipate the heat in the center of the migration chamber 2, so a temperature difference occurs between the buffer solution at the center and both ends of the migration chamber 2.
The buffer solution at the center of the migration chamber 2, which has the highest temperature, flows upward, and the buffer solution at both ends of the migration chamber 2 flows downward, circulating within the migration chamber 2. When a charged liquid such as protein is supplied from the inlet nozzle 6 into the electrophoresis chamber 2 where the buffer solution circulates, the inside of the electrophoresis chamber 2 changes depending on whether the charged substance is positively or negatively charged. It moves in the direction of the negative or positive electrode chamber 3b or 3a while flowing due to convection. In this case, when the charging electrode 5a is positive and the charging electrode 5b is negative and a DC voltage is applied, the positively charged charged substance moves to the electrode chamber 3b side and is deposited Wkm in the lower part of the electrode chamber 3b side in the migration chamber 2. , is taken out from the outlet nozzle 7b as a processing liquid. Similarly, the negatively charged charged substance moves to the electrode chamber 3a side, is concentrated in the lower part of the electrode chamber 3a side in the migration chamber 2, and is taken out from the outlet nozzle 7a as a processing liquid. As a result, it is possible to continuously separate the plurality of charged substances according to their charged states, that is, whether they are positively or negatively charged.
また、荷電物質は泳動室２内中央より両端に向って循環
するバッファ液の対流により、泳動室２内両端部を下向
きに移動しながら濃縮され、泳動室２の両端下部より取
出されるので、荷電物質の濃度が高くなった場合でも、
容易に分離することができ、高濃縮することができる。In addition, the charged substance is concentrated while moving downward at both ends of the migration chamber 2 due to the convection of the buffer solution circulating from the center of the migration chamber 2 toward both ends, and is taken out from the bottom of both ends of the migration chamber 2. Even when the concentration of charged substances increases,
It can be easily separated and highly concentrated.
このような複数の荷電物質を含む被処理液より、電気泳
動法により荷電物質を分離するためには、分離すべき荷
電物質の帯電を逆にする必要があるが、例えば等電点７
・２のミオグロビンと、等電点１１のリゾチームとを分
離する場合Ｉｅは、泳′動用バッファ液のＰＨを９・Ｏ
とすることにより、それぞれのたんばく質の帯電を正、
負両極Ｉこ分けることができる。In order to separate a charged substance from a liquid to be processed containing a plurality of charged substances by electrophoresis, it is necessary to reverse the charge of the charged substances to be separated.
・When separating myoglobin (2) and lysozyme (isoelectric point 11), the pH of the electrophoresis buffer solution should be adjusted to 9.0
By setting the charge of each protein to positive,
It can be divided into two negative poles.
第３図は本発明の他の実施例を示したもので、第１図、
第２図と同一部分は同一符号で示し、説明を省略する。FIG. 3 shows another embodiment of the present invention, and FIG.
The same parts as in FIG. 2 are indicated by the same reference numerals, and their explanation will be omitted.
第３図において、１０ａ、１０ｂは電極室３ａ、３ｂ内
の温度を調節するための温度調節器、１１ａ、１１ｂは
温度調節器１０ａ。In FIG. 3, 10a and 10b are temperature regulators for adjusting the temperature inside the electrode chambers 3a and 3b, and 11a and 11b are temperature regulators 10a.
１０ｂで温度調節されたバッファ液をそれぞれ電極室３
ａ、３ｂに循環させる送液ポンプであって、電極室３ａ
、３ｂ内のバッファ液を抜出し、温度調節器１０ａ、１
０ｂで所定温度に冷却した後、電極室３ａ、３ｂに循環
させて、電極室３ａ、３ｂを強制冷却することにより、
泳動室２内のノイツファ液および被処理液の対流を助長
すると共に、泳動室２内の液温を所定温度１こ保持して
、対流１こよる循環流を安定させることができる。10b, the temperature-controlled buffer solution is transferred to the electrode chamber 3.
a, 3b, and is a pump for circulating liquid to electrode chamber 3a.
, 3b is extracted, and the temperature controllers 10a, 1
After cooling to a predetermined temperature at 0b, it is circulated to the electrode chambers 3a, 3b to forcefully cool the electrode chambers 3a, 3b.
It is possible to promote convection between the Neutffer liquid and the liquid to be processed in the migration chamber 2, maintain the liquid temperature in the migration chamber 2 at a predetermined temperature, and stabilize the circulating flow due to the convection.
本実施例においては、荷電物質の分離に必要な泳動室２
内の対流を、電極室３ａ、３ｂ内のバッファ液温度を調
節することによって得ることができ、荷電物質の分離効
率を向上させることができる。In this example, the electrophoresis chamber 2 necessary for separating charged substances is
The convection within the electrode chambers 3a and 3b can be obtained by adjusting the temperature of the buffer solution within the electrode chambers 3a and 3b, thereby improving the separation efficiency of charged substances.
第４図は本発明の更に他の実施例を示したもあで、泳動
室２内の下部中央１こ垂直方向に仕切板１２を設置して
、泳動室２内の上昇対流を安定させると共に、泳動室２
内の電極室３ａ、３ｂ側下部に泳動分離された荷電物質
の一部が中央部に流動して再混合れるのを防止して、荷
電物質の分離効率向上させるようにしたものである。FIG. 4 shows still another embodiment of the present invention, in which a partition plate 12 is installed vertically in the center of the lower part of the migration chamber 2 to stabilize the upward convection within the migration chamber 2. , migration chamber 2
This prevents a part of the charged substances electrophoretically separated in the lower part of the electrode chambers 3a and 3b from flowing to the center and being remixed, thereby improving the separation efficiency of the charged substances.
第５図は本発明の更に他の実施例を示したもので、泳動
室２内の下部中央に垂直方向にジャケット付仕切板１３
を設置し、ジャケット付仕切板１３内に熱媒を流通させ
て泳動室２内中央部の液温を所定温度に保持することに
より、安定した対流を得るよう番こしたものであり、こ
れによって、分離効率を更に向上させるものである。す
なわち、泳動室２内の中央部と両端部の温度差を常時一
定値に保つことにより、一定した流速の対流を得ること
ができ、泳動室２内の液の乱れを小さくして分離性能を
高めることができる。FIG. 5 shows still another embodiment of the present invention, in which a jacketed partition plate 13 is arranged vertically at the center of the lower part of the migration chamber 2.
The liquid temperature in the center of the migration chamber 2 is maintained at a predetermined temperature by distributing a heat medium through the jacketed partition plate 13 to obtain stable convection. , which further improves separation efficiency. In other words, by keeping the temperature difference between the center and both ends of the migration chamber 2 at a constant value, convection with a constant flow rate can be obtained, reducing turbulence of the liquid in the migration chamber 2 and improving separation performance. can be increased.
第６図は本発明の更１こ他の実施例を示したもので、泳
動室２内の下部中央１こ垂直方向に仕切板１２を設置し
、仕切板１．２の両側に気泡発生用ノズル１４ａ、１４
ｂを設け、泳動室２の上端に気体出口ノズル１５を設け
て、気泡発生用ノズル１４ａ。FIG. 6 shows another embodiment of the present invention, in which a partition plate 12 is installed vertically in the lower center of the migration chamber 2, and air bubble generation is provided on both sides of the partition plate 1.2. Nozzles 14a, 14
b, and a gas outlet nozzle 15 is provided at the upper end of the migration chamber 2 to form a bubble generating nozzle 14a.
１４ｂより泳動室２内番こ連続的に気泡１６を発生させ
、泳動室２内中央部を上昇させることにより、泳動室２
内の循環流れを促進させて分離性能を高めるよう１こし
たものである。By continuously generating air bubbles 16 in the migration chamber 2 from 14b and raising the central part of the migration chamber 2, the migration chamber 2
It is strained to promote circulation flow within the tank and improve separation performance.
木実施例では気泡ポンプ作用による泳動室２内の対流促
進について説明したが、泳動室２内の対流を強制的に促
進させる手段としては、水中ポンプなど機械的な液体移
送装置を用いることもできる。In the embodiment described above, the promotion of convection within the migration chamber 2 through the action of a bubble pump has been described, but as a means for forcibly promoting convection within the migration chamber 2, a mechanical liquid transfer device such as a submersible pump may also be used. .
未発明は以上述べたように、泳動室の両側より直流電圧
を荷電し、泳動室内のバッファ液に泳動室中央部で上昇
し両端部で下降する循環流を形成させ、泳動室内に被処
理液を供給して電気泳動１こより荷電物質を分離し、処
理液を泳動室両端下部より取出すようにしたものである
から、バッファ液中Ｉと電流を流すことにより発生する
ジュール熱によるバッファ液の対流を利用することがで
き、かつ、荷電物質を安定して連続的に処理することが
できるため、荷電物質の分離性能を向上させることがで
きると共に大量処理が可能である。また、処理液を泳動
室両端下部から取出すことにより、荷電物質の対流拡散
を防止して高濃縮することができる。As described above, in the uninvention, a DC voltage is applied from both sides of the electrophoresis chamber to form a circulating flow in the buffer solution in the electrophoresis chamber, which rises in the center of the electrophoresis chamber and descends at both ends. Charged substances are separated from electrophoresis chamber 1 by supplying I, and the processing solution is taken out from the bottom of both ends of the electrophoresis chamber, so convection of the buffer solution due to Joule heat generated by flowing current between I in the buffer solution can be used and charged substances can be processed stably and continuously, so that separation performance of charged substances can be improved and large-scale processing is possible. Further, by taking out the processing liquid from the lower part of both ends of the electrophoresis chamber, it is possible to prevent the charged substance from convection diffusion and to highly concentrate it.
第１図は本発明の一実を例を示す電気泳動装置の正面図
、第２図は第１図のＩＩ−ｎ断面図、第３図ないし第６
図はそれぞれ本発明の他の実施例を示す電気泳動装置の
正面図である。
１・・・・・・泳動装置本体、２・・・・・・泳動室、
３ａ、３ｂ・・・・・・電極室、４・・・・・・半透膜
、５ａ、５ｂ・・・・・・荷電用電極、６，８ａ、８ｂ
・・・・・・入口ノズル、７ａ。
７ ｂ、 ９ ａ、 ９ ｂ−出口ノズル、１０ａ
、１０ｂ・・・・・・温度調節器、ｌｌａ、ｌｌｂ・・
・・・・送液ポンプ、１２・・・・・・仕切板、１３・
・・・・・ジャケット付仕切板、１４ａ、１４ｂ・・・
・・・ネ泡発生用ノズル、１５・・・・・・気体出口ノ
ズル、１６・・・・・・気泡才１図
第３図FIG. 1 is a front view of an electrophoresis device showing an example of the present invention, FIG. 2 is a sectional view taken along line II-n in FIG. 1, and FIGS.
Each figure is a front view of an electrophoresis apparatus showing other embodiments of the present invention. 1...Migration device main body, 2...Migration chamber,
3a, 3b... Electrode chamber, 4... Semipermeable membrane, 5a, 5b... Charging electrode, 6, 8a, 8b
...Inlet nozzle, 7a. 7b, 9a, 9b - outlet nozzle, 10a
, 10b...Temperature controller, lla, llb...
...Liquid pump, 12...Partition plate, 13.
...Partition plate with jacket, 14a, 14b...
...Bubble generation nozzle, 15...Gas outlet nozzle, 16...Bubble generation Figure 1 Figure 3
Claims (1)
により処理して荷電物質を分離する無担体連続電気泳動
方法において、泳動室の両側より直流電圧を荷電し、泳
動室内のバッファ液を泳動室中央部で上昇し両端部で下
降するように循環させながら、泳動室内に被処理液を供
給して荷電物質を分離し、処理液を泳動室両端下部より
取出すことを特徴とする無担体連続電気泳動方法。 ２、前記泳動室の両端部を冷却するようにした特許請求
の範囲第１項記載の無担体連続電気泳動方法。 ３、複数の荷電物質を含む被処理液を無担体電気泳動法
により処理して荷電物質を分離する無担体連続電気泳動
装置において、泳動室の両端に半透膜を介して電極室を
形成した泳動装置本体を垂直に設置し、前記泳動室に被
処理液の入口ノズルを設け、前記泳動室両端下部に処理
液の出口ノズルを設けたことを特徴とする無担体連続電
気泳動装置。 ４、前記両電極室にそれぞれ冷却されたバッファ液を循
環させる温度調節器と送液ポンプを設けた特許請求の範
囲第３項記載の無担体連続電気泳動装置。 ５、前記泳動室内下部中央に垂直方向に仕切板を設置し
た特許請求の範囲第３項又は第４項記載の無担体連続電
気泳動装置。 ６、前記泳動室の仕切板両側下部に気泡発生用ノズルを
設置した特許請求の範囲第５項記載の無担体連続電気泳
動装置。 ７、前記泳動室の下部中央に垂直方向に内部に熱媒を流
通させるジャケット付仕切板を設置した特許請求の範囲
第３項又は第４項記載の無担体連続電気泳動装置。[Claims] 1. In a carrier-free continuous electrophoresis method in which a liquid to be processed containing a plurality of charged substances is processed by a carrier-free electrophoresis method to separate charged substances, a DC voltage is applied from both sides of the electrophoresis chamber. While circulating the buffer solution in the electrophoresis chamber so that it rises in the center of the electrophoresis chamber and descends at both ends, the liquid to be processed is supplied into the electrophoresis chamber to separate charged substances, and the treated liquid is taken out from the bottom of both ends of the electrophoresis chamber. A carrier-free continuous electrophoresis method characterized by: 2. The carrier-free continuous electrophoresis method according to claim 1, wherein both ends of the electrophoresis chamber are cooled. 3. In a carrier-free continuous electrophoresis device in which a liquid to be processed containing multiple charged substances is processed by carrier-free electrophoresis to separate the charged substances, electrode chambers were formed at both ends of the electrophoresis chamber via semipermeable membranes. 1. A continuous carrier-free electrophoresis device, characterized in that a main body of the electrophoresis device is installed vertically, an inlet nozzle for a liquid to be processed is provided in the electrophoresis chamber, and outlet nozzles for a process liquid are provided at the bottom of both ends of the electrophoresis chamber. 4. The carrier-free continuous electrophoresis apparatus according to claim 3, further comprising a temperature controller and a liquid sending pump for circulating cooled buffer solutions in both electrode chambers. 5. The carrier-free continuous electrophoresis apparatus according to claim 3 or 4, wherein a partition plate is installed vertically in the center of the lower part of the electrophoresis chamber. 6. The carrier-free continuous electrophoresis apparatus according to claim 5, wherein bubble generating nozzles are installed at the bottom of both sides of the partition plate of the migration chamber. 7. The carrier-free continuous electrophoresis device according to claim 3 or 4, wherein a jacketed partition plate is installed at the center of the lower part of the migration chamber to allow a heating medium to flow therein in a vertical direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59204002A JPH0625749B2 (en) | 1984-10-01 | 1984-10-01 | Carrier-free continuous electrophoresis method and apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59204002A JPH0625749B2 (en) | 1984-10-01 | 1984-10-01 | Carrier-free continuous electrophoresis method and apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6183952A true JPS6183952A (en) | 1986-04-28 |
| JPH0625749B2 JPH0625749B2 (en) | 1994-04-06 |
Family
ID=16483145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59204002A Expired - Lifetime JPH0625749B2 (en) | 1984-10-01 | 1984-10-01 | Carrier-free continuous electrophoresis method and apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0625749B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012002771A (en) * | 2010-06-21 | 2012-01-05 | Hitachi Chem Co Ltd | Method of concentrating ion substance of liquid extract provided for simple microanalysis in environmental field and liquid extract concentration kit therefor |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3168290B1 (en) * | 2014-07-07 | 2021-12-08 | Logos Biosystems, Inc. | Apparatus for clearing tissue using electrophoresis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5814048A (en) * | 1981-07-20 | 1983-01-26 | Atoo Kk | Clinical inspection with carrier-free electrophoresis apparatus |
| JPS58147639A (en) * | 1982-02-26 | 1983-09-02 | Hideyuki Nishizawa | Separation by continuous electric migration and its device |
| JPS5952743A (en) * | 1982-09-20 | 1984-03-27 | Atoo Kk | Method and apparatus for imparting hydrogen ion concentration gradient, migrating and dispensing hydrogen ion in noncarrier thin laminar flow isoelectric point electrophresis |
-
1984
- 1984-10-01 JP JP59204002A patent/JPH0625749B2/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5814048A (en) * | 1981-07-20 | 1983-01-26 | Atoo Kk | Clinical inspection with carrier-free electrophoresis apparatus |
| JPS58147639A (en) * | 1982-02-26 | 1983-09-02 | Hideyuki Nishizawa | Separation by continuous electric migration and its device |
| JPS5952743A (en) * | 1982-09-20 | 1984-03-27 | Atoo Kk | Method and apparatus for imparting hydrogen ion concentration gradient, migrating and dispensing hydrogen ion in noncarrier thin laminar flow isoelectric point electrophresis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012002771A (en) * | 2010-06-21 | 2012-01-05 | Hitachi Chem Co Ltd | Method of concentrating ion substance of liquid extract provided for simple microanalysis in environmental field and liquid extract concentration kit therefor |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0625749B2 (en) | 1994-04-06 |
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