JPS6182105A - Setting device for initial position of fine stylus or the like - Google Patents
Setting device for initial position of fine stylus or the likeInfo
- Publication number
- JPS6182105A JPS6182105A JP20476184A JP20476184A JPS6182105A JP S6182105 A JPS6182105 A JP S6182105A JP 20476184 A JP20476184 A JP 20476184A JP 20476184 A JP20476184 A JP 20476184A JP S6182105 A JPS6182105 A JP S6182105A
- Authority
- JP
- Japan
- Prior art keywords
- differential
- zero
- cross point
- initial position
- stylus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/002—Measuring arrangements characterised by the use of optical techniques for measuring two or more coordinates
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Length Measuring Devices By Optical Means (AREA)
Abstract
Description
【発明の詳細な説明】
(イ)産業上の利用分野
本発明は微小針等の初期位置設定装置に関し、例えばマ
イクロマニピユレーション等において、微小針等を初期
位置に設定するのに利用されるものである。DETAILED DESCRIPTION OF THE INVENTION (A) Field of Industrial Application The present invention relates to an initial position setting device for microneedles, etc., and is used for setting microneedles to initial positions in, for example, micromanipulation. It is something.
(ロ)従来技術
例えばマイクロマニピュレータで細胞等の微小物体を操
作する場合、まずその試料を保持している容器内の顕微
鏡視野内へ微小ガラス針等の操作器具を挿入しなければ
ならない。ところが、この微小針の先端は直径が数μm
以下と非常に細く、プレパラートなどに触れただけでも
折れてしまう。(B) Prior Art When a microscopic object such as a cell is manipulated using a micromanipulator, for example, a manipulation instrument such as a microscopic glass needle must first be inserted into the field of a microscope in a container holding the sample. However, the tip of this microneedle has a diameter of several μm.
It is extremely thin and will break if you touch it with a prepared slide.
従来はこの微小針の初期位置設定は目測に頼って行って
おり、マイクロマニピュレータの操作に熟練した人でな
いと困難であった。Conventionally, setting the initial position of the microneedles has been done by visual measurement, which is difficult unless someone is skilled in operating a micromanipulator.
(ハ)発明の目的
本発明は上記従来技術の欠点を解消し、マイクロマニピ
ユレーション等において微小針環ヲ顕微鏡視野内の初期
位置に自動的に確実に設定することができる微小針等の
初期位置設定装置の提供を目的とする。(c) Purpose of the Invention The present invention solves the drawbacks of the above-mentioned prior art, and enables the initialization of microneedles, etc., which can automatically and reliably set the microneedle ring at the initial position within the field of view of a microscope in micromanipulation, etc. The purpose is to provide a position setting device.
(ニ)発明の構成
本発明は、LED等の発光素子と差動光検出素子等から
なる差動光検出器と、前記発光素子からの光束を垂直に
横切るように微小針等の微小体を移動させる手段と、前
記微小針等の微小体の移動にともなう差動光検出器の出
力を読みとる手段とを備え、前記微小体の移動による差
動光検出器のS字状の出力波形のゼロクロス点を予め設
定すべき初期位置に定めておくことを特徴とする微小針
等の初期位置設定装置である。(d) Structure of the Invention The present invention provides a differential photodetector including a light emitting element such as an LED, a differential photodetection element, etc., and a microscopic body such as a microneedle so as to perpendicularly cross the light beam from the light emitting element. and a means for reading the output of the differential photodetector as the microscopic object such as the microneedle moves, and detects the zero cross of the S-shaped output waveform of the differential photodetector due to the movement of the microscopic object. This is an initial position setting device for microneedles, etc., characterized by setting a point at an initial position to be set in advance.
(ホ)実施例
第1図は本発明の実施例を示す構成図、第2図は差動光
検出器の平面図、第3図は差動光検出器の正面図、第4
図は差動光検出器による出力波形の例を示す図である。(E) Embodiment FIG. 1 is a configuration diagram showing an embodiment of the present invention, FIG. 2 is a plan view of a differential photodetector, FIG. 3 is a front view of the differential photodetector, and FIG.
The figure is a diagram showing an example of an output waveform from a differential photodetector.
顕微鏡のステージ6上に差動光検出器1を設置し、微小
ガラス針3等の微小体を移動させる手段であるマイクロ
マニピュレータ2により、図のy軸方向に微小ガラス針
3を差動光検出器1に挿入し、さらに微小ガラス針3を
差動光検出器1に対して図のX軸方向とX軸方向を移動
させることにより、顕微鏡視野内に入るX座標と2座標
の位置を検出し、そのX座標と2座標の決定された位置
でさらに微小ガラス針3をy軸方向へ直線的に移動させ
れば、操作容器4等に接触することなく微小ガラス針3
を顕微鏡の視野内に入れることができる。5は顕微鏡の
対物レンズである。A differential photodetector 1 is installed on a microscope stage 6, and a micromanipulator 2, which is a means for moving a microscopic object such as a microscopic glass needle 3, detects the microscopic glass needle 3 in the y-axis direction in the figure. By inserting the microscopic glass needle 3 into the device 1 and moving the microscopic glass needle 3 in the X-axis direction and the X-axis direction shown in the figure with respect to the differential photodetector 1, the position of the X coordinate and 2 coordinates that fall within the field of view of the microscope is detected. However, if the micro glass needle 3 is further linearly moved in the y-axis direction at the determined position of the X coordinate and 2 coordinates, the micro glass needle 3 can be moved straightly in the y-axis direction without contacting the operation container 4 etc.
can be brought into the field of view of the microscope. 5 is an objective lens of the microscope.
装置の構成についてさらに説明すると、前記顕i鏡のス
テージ6に設置される差動光検出器1はX軸方向に離間
して設けたLED等の発光素子11と差動太陽電池等の
差動光検出素子12の対と、2軸方向に離間して設けた
発光素子13と差動光検出素子14の対とを設けている
。よって微小ガラス針3を2軸方向に移動させば対11
.12により第4図に示す如きS字状の出力波形を得る
ことができ、ゼロクロス点の2座標zOを知ることがで
きる。また微小ガラス針3をX軸方向に移動させば対1
3.14により同様に第4図に示す如きS字状の出力波
形を得てゼロクロス点のX座標XQを知ることができる
。To further explain the configuration of the device, a differential photodetector 1 installed on the stage 6 of the microscope has a light emitting element 11 such as an LED spaced apart in the X-axis direction and a differential photodetector such as a differential solar cell. A pair of photodetecting elements 12 and a pair of a light emitting element 13 and a differential photodetecting element 14 provided spaced apart in two axial directions are provided. Therefore, if the micro glass needle 3 is moved in two axial directions, pair 11
.. 12, an S-shaped output waveform as shown in FIG. 4 can be obtained, and two coordinates zO of the zero cross point can be found. In addition, if the micro glass needle 3 is moved in the X-axis direction, the pair 1
3.14, it is also possible to obtain an S-shaped output waveform as shown in FIG. 4 and find the X coordinate XQ of the zero crossing point.
したがって、逆にこのゼロクロス点(xo、zo)が顕
微鏡視野に入る領域の一点に該当するX座標と2座標に
なるように、予め差動光検出器1をステージ6上に設置
しておけば、操作時にこのゼロクロス点を検出するよう
微小ガラス針3を移動させるだけで、顕微鏡視野内に挿
入することができる微小ガラス針3のX、2座標を得る
ことができる。顕微鏡視野内のy座標は顕微鏡をみなが
ら微小ガラス針3をy軸方向に移動させることにより好
みの位置y】に設定することができる。すなわち微小ガ
ラス針3の初期位置として(xo、yt。Therefore, conversely, if the differential photodetector 1 is installed on the stage 6 in advance so that this zero crossing point (xo, zo) is at two coordinates with the X coordinate corresponding to one point in the field of view of the microscope, By simply moving the glass micro needle 3 to detect this zero-crossing point during operation, the X and 2 coordinates of the micro glass needle 3 that can be inserted into the field of view of the microscope can be obtained. The y-coordinate within the field of view of the microscope can be set to a desired position y by moving the microscopic glass needle 3 in the y-axis direction while viewing the microscope. That is, the initial position of the micro glass needle 3 is (xo, yt.
20)を得ることができる。初期位置設定を自動的に行
うため、第1図に示すように、差動光検出器1の出力を
読みとる出力読取器7と、出力読取器7による出力波形
からゼロクロス点に微小ガラス針3が移動したことを判
別する判別器8を組合せて装置を構成する。20) can be obtained. In order to automatically set the initial position, as shown in FIG. A device is configured by combining a discriminator 8 that determines whether the device has moved.
(へ)効果
本発明は以上の構成よりなり、微小針等の微小体を設定
すべき初期位置に容易に速やかに移動させることができ
る。また発光素子と差動光検出素子等からなる差動光検
出器を用いているので微小針等を非常に正確に位置設定
できる。したがって本装置をマイクロマニピュレータの
粗動機構制御系等に組み入れることが微小針等の初期位
置設定が非常に容易になる。(F) Effect The present invention has the above-described configuration, and can easily and quickly move a microscopic body such as a microneedle to an initial position to be set. Furthermore, since a differential photodetector consisting of a light emitting element and a differential photodetection element is used, the position of a microneedle etc. can be set very accurately. Therefore, by incorporating this device into a coarse movement mechanism control system of a micromanipulator, it becomes very easy to set the initial position of a microneedle, etc.
第1図は本発明の実施例を示す構成図、第2図は差動光
検出器の平面図、第3図は差動光検出器の正面図、第4
図は差動光検出器による出方波形の例を示す図である。
1−・・差動光検出器
2−・−マイクロマニピュレータ
3−・微小ガラス針
4・−・操作容器
5− ・対物レンズ
6−・ステージ
7−・出力読取器
8・−判別器
11.13−・発光素子FIG. 1 is a configuration diagram showing an embodiment of the present invention, FIG. 2 is a plan view of a differential photodetector, FIG. 3 is a front view of the differential photodetector, and FIG.
The figure is a diagram showing an example of an output waveform by a differential photodetector. 1--Differential photodetector 2--Micromanipulator 3--Micro glass needle 4--Operation container 5--Objective lens 6--Stage 7--Output reader 8--Discriminator 11.13 -・Light emitting element
Claims (1)
検出器と、前記発光素子からの光束を垂直に横切るよう
に微小針等の微小体を移動させる手段と、前記微小針等
の微小体の移動にともなう差動光検出器の出力を読みと
る手段とを備え、前記微小体の移動による差動光検出器
のS字状の出力波形のゼロクロス点を予め設定すべき初
期位置に定めておくことを特徴とする微小針等の初期位
置設定装置。A differential photodetector consisting of a light emitting element such as an LED and a differential light detection element, a means for moving a microscopic body such as a microneedle so as to perpendicularly cross the light beam from the light emitting element, and a means for moving a microscopic body such as a microneedle, etc. means for reading the output of the differential photodetector as the microscopic object moves, and sets the zero-crossing point of the S-shaped output waveform of the differential photodetector due to the movement of the microscopic object to a preset initial position. An initial position setting device for a microneedle, etc.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP20476184A JPS6182105A (en) | 1984-09-29 | 1984-09-29 | Setting device for initial position of fine stylus or the like |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP20476184A JPS6182105A (en) | 1984-09-29 | 1984-09-29 | Setting device for initial position of fine stylus or the like |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6182105A true JPS6182105A (en) | 1986-04-25 |
Family
ID=16495906
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP20476184A Pending JPS6182105A (en) | 1984-09-29 | 1984-09-29 | Setting device for initial position of fine stylus or the like |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6182105A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11885099B2 (en) | 2018-10-17 | 2024-01-30 | Caterpillar Sarl | Drift-prevention valve device, blade device, and working machine |
-
1984
- 1984-09-29 JP JP20476184A patent/JPS6182105A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11885099B2 (en) | 2018-10-17 | 2024-01-30 | Caterpillar Sarl | Drift-prevention valve device, blade device, and working machine |
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