JPS6143981A - Continuous flow homogenizer - Google Patents
Continuous flow homogenizerInfo
- Publication number
- JPS6143981A JPS6143981A JP16421484A JP16421484A JPS6143981A JP S6143981 A JPS6143981 A JP S6143981A JP 16421484 A JP16421484 A JP 16421484A JP 16421484 A JP16421484 A JP 16421484A JP S6143981 A JPS6143981 A JP S6143981A
- Authority
- JP
- Japan
- Prior art keywords
- pestle
- container
- tissue
- coupling
- tubular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/08—Homogenizing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Disintegrating Or Milling (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野〕
本発明は、細胞膜を破砕して細胞内の小器官を分離する
ために用いるホモジナイザに関し、構成簡単で安価に製
作できると共に破砕効率高く、しかも、取扱いに便利な
連続流動式ホモジナイザに関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a homogenizer used to disrupt cell membranes and separate intracellular organelles, which has a simple configuration, can be manufactured at low cost, and has high disruption efficiency. , relates to a continuous flow homogenizer that is convenient to handle.
［従来技術の説明］
生物、医学、その他の研究分野において、細胞内のゴル
ジ体、ミトコンドリア等の小器官、その他酵素等を分離
することが必要である。このためには何らかの手段をも
って細胞を破砕しなければならないのであるが、従来の
細胞膜破砕装置として、超音波を利用するもの、高圧窒
素ガス等により圧力をかけこの圧力を急激に除去しＩで
細胞膜を破砕するもの、又、油圧機械により細胞液に圧
力をかけ細孔を通過させて細胞膜を破砕する方式などを
始めとし、ボッタ型と呼ばれ、管状容器内の乳棒をスタ
ークで回転させながら往復運動を与えて細胞を分断する
方式、又既設の回転機を用いて管状容器内の乳棒を回転
させること等も行なわれている。[Description of Prior Art] In biology, medicine, and other research fields, it is necessary to separate intracellular Golgi bodies, organelles such as mitochondria, and other enzymes. To do this, cells must be disrupted by some means, but conventional cell membrane disruption devices use ultrasonic waves, apply pressure with high-pressure nitrogen gas, etc., and rapidly remove this pressure. There are also methods that crush cell membranes by applying pressure to the cell fluid using a hydraulic machine and passing it through pores, called the Botta type. Methods of dividing cells by applying motion, and methods of rotating a pestle inside a tubular container using an existing rotary machine have also been used.
［従来技術の問題点］
これら従来より細胞膜破砕装置は、慨して破砕効率が悪
く、又、取扱いに不便であった。例えば、広く普及して
いるボツタ型においては管状体容器を手動で上下動させ
ねばならず非能率的である。[Problems with the Prior Art] These conventional cell membrane disruption devices generally have poor disruption efficiency and are inconvenient to handle. For example, in the widely popular pot type, the tubular container must be moved up and down manually, which is inefficient.
又例えば、その他の超音波や高圧窒素を用いたものでは
装置が大型となり高価な装置となっている。For example, other devices using ultrasonic waves or high-pressure nitrogen require large and expensive devices.
［問題点解決のための手段］
本発明は上記従来技術の問題点を解決するもので装置の
構成が簡単であり安価に製作できると共に破砕効率高く
、しかも、取扱いに便利な細胞膜破砕用のホモジナイザ
を提供するもので、そのホモジナイザの特徴とするとこ
ろは、上端に未乳化組織導入口を有すると共に下端に乳
化組織排出口を有する管状体容器を着脱自在に支柱に支
持して設け、該管状体容器の内部に、回転軸を備えた乳
棒を適宜の細隙をおいて回転自在に配設する一方、前記
支柱上端に備えた回転駆動部から回転駆動力を受けで前
記管状体容器の上端に向って垂架される回転駆動軸を設
け、該回転駆動軸と前記乳棒に備えた回転軸とをカップ
リングを介して着脱自在に接合したことにある。[Means for Solving the Problems] The present invention solves the problems of the prior art described above, and provides a homogenizer for cell membrane disruption that has a simple device configuration, can be manufactured at low cost, has high disruption efficiency, and is convenient to handle. The homogenizer is characterized by having a tubular container having an unemulsified tissue inlet at the upper end and an emulsified tissue outlet at the lower end removably supported on a support. A pestle equipped with a rotating shaft is rotatably disposed inside the container with an appropriate gap, and a pestle is placed at the upper end of the tubular container by receiving a rotational driving force from a rotational driving section provided at the upper end of the column. A rotary drive shaft is provided vertically facing the pestle, and the rotary drive shaft and the rotary shaft provided on the pestle are removably connected to each other via a coupling.
［作用］
上記構成の細胞膜破砕用のホモジナイザを使用するに際
しては、先ず、例えば動物質供試材料をミンチ状とし、
緩衝液を加えて細胞液を得、該細胞液を、支柱に支持さ
れたその内部に回転可能の乳棒を配設された管状体容器
の上端の未乳化組織導入口に注ぎ、しかる後前記乳棒を
回転駆動軸を介して回転させる。[Operation] When using the homogenizer for disrupting cell membranes having the above configuration, first, for example, mince an animal sample material,
A buffer solution is added to obtain a cell solution, and the cell solution is poured into an unemulsified tissue inlet at the upper end of a tubular container supported by a column and equipped with a rotatable pestle. is rotated via a rotating drive shaft.
すると、前記細胞液は管状体容器と乳棒との間で乳化さ
れ細胞膜が破砕されて前記管状体容器の底部排出口から
順次排出されることになる。支ｌに支持した管状体容器
の取付け、又は取外し作業は、乳棒に備えた回転軸と、
該回転軸に接続される回転駆動軸とをカップリングを介
して着脱自在に接続しているので、このカップリングを
外すことにより、管状体容器から乳棒を完全に抜きとる
こと無く管状体容器を着脱することが容易に可能である
。Then, the cell fluid is emulsified between the tubular container and the pestle, the cell membranes are crushed, and the cell fluid is sequentially discharged from the bottom outlet of the tubular container. The work of attaching or removing the tubular body container supported on the support l is carried out using the rotary shaft provided on the pestle,
The rotary shaft is removably connected to the rotating shaft via a coupling, so by removing this coupling, the tubular container can be removed without completely removing the pestle from the tubular container. It can be easily attached and detached.
又、前記管状体容器の上端には未乳化組織導入口を設け
ているので、この導入口から連続して未乳化組織液を導
入し連続した乳化操作を行うことができるので、乳化作
業を極めて効率良く行うことができる。In addition, since an unemulsified tissue inlet is provided at the upper end of the tubular container, unemulsified tissue fluid can be continuously introduced through this inlet and a continuous emulsification operation can be performed, making the emulsification work extremely efficient. can do well.
［実施例の説明］
以下、本発明の一実施例を図面を用いて詳細に説明する
。[Description of Embodiment] Hereinafter, one embodiment of the present invention will be described in detail using the drawings.
第１図は連続流動式ホモジナイザの装置全体を示す斜視
図、第２図は乳棒を備えた管状体容器の断面図、第３図
はカップリングの詳細図である。FIG. 1 is a perspective view of the entire continuous flow homogenizer, FIG. 2 is a sectional view of a tubular container equipped with a pestle, and FIG. 3 is a detailed view of the coupling.
第１図は、基台１に立設した支柱３に、上端に未乳化組
織導入口５を有すると共に下端に乳化組織排出ロアを有
する透明の管状体容器９を着脱自在に支持して設け、該
管状体容器９の内部に、回転軸１１を備えた乳棒１３を
前記管状体容器９の内壁との間に適宜の細隙をおいて回
転自在に配設する一方、前記支柱３上端に備えた回転駆
動部１５から回転駆動力を受けて前記管状体容器９の上
端に向って垂架される回転駆動軸１７を設け、該回転駆
動軸１７と前記乳棒１３に備えた回転軸１１とをカップ
リング１９を介して着脱自在に接合した連続流動式ホモ
ジナイザを示している。In FIG. 1, a transparent tubular container 9 having an unemulsified tissue inlet 5 at the upper end and an emulsified tissue discharge lower at the lower end is removably supported on a support 3 erected on a base 1. A pestle 13 having a rotating shaft 11 is rotatably disposed inside the tubular container 9 with an appropriate gap between it and the inner wall of the tubular container 9, and a pestle 13 is provided at the upper end of the support 3. A rotary drive shaft 17 is provided which receives a rotary drive force from a rotary drive unit 15 and is suspended vertically toward the upper end of the tubular body container 9, and the rotary drive shaft 17 and the rotary shaft 11 provided on the pestle 13 are connected to each other. A continuous flow homogenizer is shown removably connected via a coupling 19.
基台１と支柱３とは、例えば実験用スタンドとして市販
されているものをそのまま使用することもできるが、こ
こでは、支柱３の上方に回転駆動部１５を設けている。For the base 1 and the support column 3, for example, a commercially available experimental stand may be used as is, but here, a rotation drive unit 15 is provided above the support column 3.
２１はコンセント、２３は電圧交換部を示す。21 is an outlet, and 23 is a voltage exchange section.
又、該支柱３に管状体容器９を把持する取付金具２５．
２７を設けている。Also, a mounting bracket 25 for holding the tubular body container 9 on the support column 3.
There are 27.
取付金具２５．２７は、管状体容器９に向って開いた半
円弧状の固定部材２５ａ、２７ａと、蝶ねじ２５ｂ、２
７ｂを回動可能に備えた前記固定部材と対称の半円弧状
の開閉部材２５ｃ、２７ｃから成り、前記固定部材２５
ａ、２７ａの一端には前記蝶ねじ２５ｂ 、２７ｂのね
じ部を螺入させるタップ穴を設け、他端には開閉部材２
５ｂ、２７ｂを固定部材２５ａ、２７ａに対して回動さ
せる蝶番（図示せず）を設けている。蝶ねじ２５ｂ１２
７ｂはいわゆるバラチン錠としても良い。固定部材２５
ａ 、２７ａと開閉部材２５ｃ、２７ｃの円弧状部内側
には、それぞれゴム質等のクッション（図示せず）を設
け、管状体容器を柔軟に、がつ、確実に固定できるよう
にしている。The mounting brackets 25 and 27 include semicircular arc-shaped fixing members 25a and 27a that open toward the tubular body container 9, and thumbscrews 25b and 2.
It consists of semicircular arc-shaped opening/closing members 25c and 27c symmetrical to the fixing member which is rotatably equipped with the fixing member 25.
A, 27a is provided with a tap hole into which the threaded portion of the thumb screw 25b, 27b is screwed, and the opening/closing member 2 is provided at the other end.
A hinge (not shown) is provided for rotating the fixing members 5b and 27b relative to the fixing members 25a and 27a. Butterfly screw 25b12
7b may be a so-called baratin tablet. Fixed member 25
A cushion (not shown) made of rubber or the like is provided inside the arcuate portions of the opening/closing members 25c, 27a and the opening/closing members 25c, 27c, respectively, so that the tubular container can be fixed flexibly, firmly, and reliably.
なお、このクッションを白色、若しくはねずみ色にして
おけば、透明の管状体容器を透かして乳化状態が観察ｒ
きるので便利である。Note that if this cushion is made white or gray, the emulsified state can be observed through the transparent tubular container.
It is convenient because you can
回転駆動部１５の前面には電源ＡンＡフ用のスイッチ２
９、スタートスイッチ３１、ストップスイッチ３２、回
転速度設定用のデジタルスイッチ３３、実際の回転速度
を表示するＬＥＤ（発光ダイボード）式の速度表示装置
１３５等を設けている。On the front of the rotary drive unit 15, there is a switch 2 for power supply A/A.
9, a start switch 31, a stop switch 32, a digital switch 33 for setting the rotational speed, an LED (light emitting die board) type speed display device 135 for displaying the actual rotational speed, etc. are provided.
回転駆動軸１７は図示しない伝導機構を介して前記デジ
タルスイッチ３３で定められる速度で一方向に回転され
る。The rotary drive shaft 17 is rotated in one direction at a speed determined by the digital switch 33 via a transmission mechanism (not shown).
第２図に示すように、管状体容器９の上端部に多量の組
織溶液を受けられるように逆円錐状に開口した未乳化組
織（細胞液）導入口５を形成している。管状体容器９の
底部には乳化組織排出ロアを設けている。As shown in FIG. 2, an unemulsified tissue (cell fluid) inlet 5 is formed at the upper end of the tubular body container 9 and has an inverted conical opening to receive a large amount of tissue solution. An emulsified tissue discharge lower is provided at the bottom of the tubular body container 9.
管状体容器９はガラスで製作されたものの例を示すが、
これに限定されるものではない。An example of the tubular body container 9 made of glass is shown;
It is not limited to this.
第２図は乳棒１３を管状体容器９の略中闇位置に位置さ
せた状態を示し、この状態で、管状体容器９の上方には
未乳化組織（細胞液）を収納する第１室３５を、下方に
は乳化後の液を排出ロアに案内する第２室３７を形成し
ている。乳棒１３と管状体容器９との間の細隙は細胞核
および他の細胞器官を分離するために必要な間隔ｄ（０
，０５〜０．２１１程度）とし、細胞種に応じて定めら
れるものである。FIG. 2 shows a state in which the pestle 13 is positioned approximately in the middle of the tubular body container 9, and in this state, a first chamber 35 containing unemulsified tissue (cell fluid) is located above the tubular body container 9. A second chamber 37 is formed below to guide the emulsified liquid to the discharge lower. The gap between the pestle 13 and the tubular container 9 is the distance d(0) necessary to separate the cell nucleus and other organelles.
, 0.05 to about 0.211), and is determined depending on the cell type.
第２図において、乳棒１３に備えた回転軸１１は矢印３
９の方向に回転する。乳棒１３は例えばテフロンで製作
され、その上方外周面には回転に伴って前記第１至３５
の未乳化組織を乳棒中央部１３ａに管状体容器９の内周
面との間隔を次第に小さくしながら送給することの可能
な案内溝４１．４１を多数設けている。案内溝４１．４
１の深さは、上方では３−程度で、乳棒の中央部に行く
に従って、ゼロとなるまで次第に浅くしている。In FIG. 2, the rotating shaft 11 provided on the pestle 13 is indicated by the arrow 3.
Rotate in direction 9. The pestle 13 is made of Teflon, for example, and the above-mentioned first to 35
A large number of guide grooves 41 and 41 are provided in the central part 13a of the pestle to allow the unemulsified tissue to be fed while gradually decreasing the distance from the inner circumferential surface of the tubular body container 9. Guide groove 41.4
The depth of the pestle is approximately 3-3 at the top, and gradually becomes shallower toward the center of the pestle until it reaches zero.
乳棒１３に上記のような案内溝４１．４１を設けている
ので、乳棒１３の方向３９への回転に伴って、第１室３
５の未乳化組織が乳棒１３の下方部と組織破砕部１３ａ
に向って送給される。組織破砕ｍ１３ｄの表面と管状体
容器９の内部表面との間に捕えられた細胞は、わずかな
すきまのなかで穏やかなせん断動作を引き起こし、破断
される。Since the pestle 13 is provided with the guide grooves 41, 41 as described above, as the pestle 13 rotates in the direction 39, the first chamber 3
The unemulsified tissue of No. 5 is the lower part of the pestle 13 and the tissue crushing part 13a.
will be sent towards. The cells trapped between the surface of the tissue disruptor m13d and the inner surface of the tubular body container 9 are ruptured by causing a gentle shearing action within a small gap.
第３図にカップリング１９の詳細図を示した。A detailed view of the coupling 19 is shown in FIG.
（ａ）図は組立て状態を示す斜視図、（ｉｌ）図はその
分解斜視図である。The figure (a) is a perspective view showing an assembled state, and the figure (il) is an exploded perspective view thereof.
カップリング１９は上部継手４３と、弾性継手４５と、
下部継手４７とから成り、上部継手４３は回転駆動軸１
７と、下部継手４７は回転軸１１とそれぞれ化ビス４９
．４９を用いて固着している。The coupling 19 includes an upper joint 43, an elastic joint 45,
The upper joint 43 consists of a lower joint 47 and a rotary drive shaft 1.
7 and the lower joint 47 are connected to the rotating shaft 11 with screws 49, respectively.
．． It is fixed using 49.
上部継手４３にはその両側面に小孔を設け、下方に傾倒
して下部継手の下端面を抱え込む抱え込み部５１を備え
たクリップ５３の端部を前記小孔に嵌合して回動自在に
支承している。又、上部継手４３の下方、及び下部継手
の上方にはそれぞれ回り止めギヤ５５．５５を備えた高
さ斐（例えば５１１ＩＩｌ）の接合部５７．５７を設け
ている。The upper joint 43 has small holes on both sides thereof, and the end of the clip 53, which has a holding part 51 that tilts downward and holds the lower end surface of the lower joint, is fitted into the small hole so that it can rotate freely. I support it. Further, below the upper joint 43 and above the lower joint, there are provided joint portions 57.57 of a height (for example, 511IIl) each provided with a detent gear 55.55.
弾性継手４５はゴム（ゴム硬度７０〜８０１−１ｓ）、
或いはプラスチック等の弾性累月で製作され、その内部
には上部継手４３、下部継手４７にそれぞれ設けた接合
部５７のそれぞれの回り止めギヤ５５と嵌合する凹溝５
９．５９を上下両端に有する。The elastic joint 45 is made of rubber (rubber hardness 70 to 801-1s),
Alternatively, it is made of an elastic material such as plastic, and has a concave groove 5 therein that fits with each detent gear 55 of the joint part 57 provided in the upper joint 43 and the lower joint 47, respectively.
9.59 on both the upper and lower ends.
弾性継手４５は上部継手４３、又は下部継手４７の内一
方に固着しておいても良い。The elastic joint 45 may be fixed to either the upper joint 43 or the lower joint 47.
上部継手４３に駆動軸１７を挿入しビス４９で固定する
一方、下部継手４７に回転軸１１を挿入してごス４９で
固定している。そこで、弾性継手４５の上下両端方向か
ら前記上部継手４３及び下部継手４７をそれぞれ突合せ
、クリップ５３を下方に降せば、＜ａ ＞図に示した状
態となる。この状態において、回転軸１１は駆動軸１７
に対して、所定の角度（３度位）だけそのずれが許容さ
れることになる。The drive shaft 17 is inserted into the upper joint 43 and fixed with screws 49, while the rotating shaft 11 is inserted into the lower joint 47 and fixed with screws 49. Therefore, by butting the upper joint 43 and the lower joint 47 from both the upper and lower end directions of the elastic joint 45, and lowering the clip 53 downward, the state shown in Fig. <a> is obtained. In this state, the rotating shaft 11 is connected to the driving shaft 17.
However, a deviation of a predetermined angle (approximately 3 degrees) is allowed.
今、第１図に示したように組立てられた状態から、乳化
作業が終了して管状体容器９を取り外すものとする。こ
の場合、第３図に示したクリップ５３を回動させて抱え
込み部５１を下部継手４７から外し、回転軸１１と一体
的の乳棒１３を第３図（ｂ）図に示した寸法斐だけ下方
に下げる。しかる後、取付部材２５．２７の蝶ねじ２５
１）、２７ｂを緩めて固定部材２５ａ、２５０から開閉
部材を外し、管状体容器９を取り去れば良い。Now, it is assumed that the emulsification work is completed and the tubular body container 9 is removed from the assembled state shown in FIG. 1. In this case, the clip 53 shown in FIG. 3 is rotated to remove the holding part 51 from the lower joint 47, and the pestle 13 integrated with the rotating shaft 11 is moved downward by the dimension shown in FIG. 3(b). lower to After that, the thumbscrew 25 of the mounting member 25.27
1), 27b may be loosened, the opening/closing member may be removed from the fixing members 25a, 250, and the tubular body container 9 may be removed.
乳化作業の開始に際し、管状体容器９を取り付ける場合
はこの逆の操作をすれば良く、先ず、管状体容器９を取
付金具２５に緩く装着する。しかる後、管状体容器９の
未乳化組織導入口３５（第２図参照）の逆円錐の細径部
（首の部分）が上方の取付金具の上端部に接触するまで
管状体容器を押し下げ位置決めする。その後蝶ねじ２５
ｂ、２７ｂを確実に締付け、乳棒に取付けられた回転軸
１１と回転駆動軸１７とをカップリング１９を介して接
合しクリップ５３で固定すれば良い。When attaching the tubular body container 9 at the start of the emulsification operation, this operation may be performed in reverse. First, the tubular body container 9 is loosely attached to the mounting fitting 25. After that, the tubular container 9 is pushed down and positioned until the narrow diameter part (neck part) of the inverted cone of the unemulsified tissue inlet 35 (see Figure 2) of the tubular container 9 comes into contact with the upper end of the upper mounting bracket. do. Then thumbscrew 25
b, 27b are securely tightened, and the rotary shaft 11 attached to the pestle and the rotary drive shaft 17 are joined via the coupling 19 and fixed with the clip 53.
本例では管状体容器９の着脱に際しては、乳棒１３を前
記した寸法交だけ下方に下げて又は上げて行うことがで
きるのであるが、着脱自在のカップリング１９を設けて
いない場合には管状体容器９を固定位置の乳棒１３から
抜きとらねばならず不便である。しかるに本例において
はカップリング１９を上記の如く設けているので基台底
面と回転駆動部１５との間を所望の高さＬとして管状体
容器９の抜取りのための不要の高さを排除しているので
ある。In this example, when attaching and detaching the tubular body container 9, the pestle 13 can be lowered or raised by the above-mentioned dimension, but if the detachable coupling 19 is not provided, the tubular body container 9 The container 9 must be removed from the pestle 13 in a fixed position, which is inconvenient. However, in this example, since the coupling 19 is provided as described above, the distance between the bottom surface of the base and the rotary drive unit 15 is set to the desired height L, thereby eliminating unnecessary height for removing the tubular body container 9. -ing
なお、管状体容器の抜取り高さを不要とする他の手段と
して、本例の他、いわゆるチャックと呼ばれるものを利
用すれば良いことが考えられる。In addition to this example, it is conceivable that what is called a chuck may be used as another means for eliminating the need for the height at which the tubular body container is removed.
しかし、チャック自身には回転軸と駆動軸との振れに対
する対応手段が無く、このチャックをもってカップリン
グに代えることはできないが、これらのチャックをカッ
プリングに併せて使用することは勿論可能である。However, the chuck itself has no means for dealing with vibrations between the rotating shaft and the drive shaft, and although this chuck cannot be used in place of a coupling, it is of course possible to use these chucks in conjunction with a coupling.
［発明の効果］
本発明は上端に未乳化組織導入口を有すると共に下端に
乳化組織排出口を有する管状体容器を着脱自在に支柱に
支持して設け、該管状体容器の内部に、回転軸を備えた
乳棒を適宜の細隙をおいて回転自在に配設する一方、前
記支柱上端に備えた回転駆動部から回転駆動力を受けて
前記管状体容器の上端に向って垂架される回転駆動軸を
設け、該回転駆動軸と前記乳棒に備えた回転軸とをカッ
プリングを介して着脱自在に接合したことを特徴とする
連続流動式ホモジナイザである。[Effects of the Invention] The present invention provides a tubular container having an unemulsified tissue inlet at the upper end and an emulsified tissue outlet at the lower end and is detachably supported on a support, and has a rotating shaft inside the tubular container. A pestle equipped with a pestle is rotatably arranged with an appropriate gap, and a rotary pestle is suspended vertically toward the upper end of the tubular container by receiving a rotational driving force from a rotational driving part provided at the upper end of the pillar. The continuous flow homogenizer is characterized in that a drive shaft is provided, and the rotary drive shaft and the rotary shaft provided on the pestle are removably connected via a coupling.
従って、本装置は構成簡単であり安価に製作できると共
に乳化作業を連続的に行い効率良く、高品質の乳化液を
得ることができる。又、作業が終って次の作業に移るな
ど、装置を取り外して容器や乳棒の清掃や交換修理を行
う場合、容器の取付金具を外すとともにカップリングの
クリップの係合を外すことにより、容易に容器と乳棒を
取り出すことができるなどの利点を有するものである。Therefore, this apparatus has a simple structure and can be manufactured at low cost, and can perform emulsification work continuously and efficiently to obtain a high-quality emulsion. In addition, when you remove the device to clean, replace or repair the container or pestle, such as when moving on to the next task, you can easily remove the mounting bracket of the container and disengage the clip of the coupling. This has the advantage that the container and pestle can be taken out.
なお、本発明は前述の実施例に限定されるものではなく
、適宜の設計的変更を行うことにより前述の実施例以外
の態様によって本発明の実施を行うことも勿論可能であ
る。Note that the present invention is not limited to the embodiments described above, and it is of course possible to implement the present invention in aspects other than the embodiments described above by making appropriate design changes.
図面はいずれも実施例を示し、第１図は連続流動式ホモ
ジナイザの装置全体斜視図。
第２図は乳棒を装着した状態を示す管状体容器の断面説
明図。
第３図はカップリングの説明図を示し、（ａ）図は組立
て状態を示す斜視図、（ｂ）図はそれを分解して示す斜
視図である。
１・・・基台 ３・・・支柱５・・・未乳
化組織導入口
ア・・・乳化組織排出口 ９・・・管状体容器１１・
・・回転軸 １３・・・乳棒１５・・・回転駆
動部 １７・・・回転駆動軸１９・・・カップリン
グ
陶６展ｆ
第１図
第２図
第３図
（ａ）
第３図
（ｂ）
■The drawings all show examples, and FIG. 1 is a perspective view of the entire continuous flow homogenizer device. FIG. 2 is an explanatory cross-sectional view of the tubular container with a pestle attached. FIG. 3 shows explanatory diagrams of the coupling, where (a) is a perspective view showing an assembled state, and (b) is an exploded perspective view. 1... Base 3... Support 5... Unemulsified tissue inlet a... Emulsified tissue outlet 9... Tubular body container 11.
... Rotation shaft 13 ... Pestle 15 ... Rotation drive unit 17 ... Rotation drive shaft 19 ... Coupling ceramics 6 exhibition f Figure 1 Figure 2 Figure 3 (a) Figure 3 (b ) ■
Claims (1)
排出口を有する管状体容器を着脱自在に支柱に支持して
設け、該管状体容器の内部に、回転軸を備えた乳棒を適
宜の細隙をおいて回転自在に配設する一方、前記支柱上
端に備えた回転駆動部から回転駆動力を受けて前記管状
体容器の上端に向って垂架される回転駆動軸を設け、該
回転駆動軸と前記乳棒に備えた回転軸とをカップリング
を介して着脱自在に接合したことを特徴とする連続流動
式ホモジナイザ。A tubular container having an unemulsified tissue inlet at the upper end and an emulsified tissue outlet at the lower end is removably supported on a post, and a pestle equipped with a rotating shaft is inserted into the interior of the tubular container. The rotary drive shaft is arranged to be freely rotatable with a gap therebetween, and receives a rotary drive force from a rotary drive unit provided at the upper end of the column, and is suspended vertically toward the upper end of the tubular body container. A continuous flow homogenizer characterized in that a shaft and a rotating shaft provided on the pestle are removably connected via a coupling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16421484A JPS6143981A (en) | 1984-08-07 | 1984-08-07 | Continuous flow homogenizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16421484A JPS6143981A (en) | 1984-08-07 | 1984-08-07 | Continuous flow homogenizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6143981A true JPS6143981A (en) | 1986-03-03 |
| JPS6233865B2 JPS6233865B2 (en) | 1987-07-23 |
Family
ID=15788833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16421484A Granted JPS6143981A (en) | 1984-08-07 | 1984-08-07 | Continuous flow homogenizer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6143981A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07203882A (en) * | 1994-01-18 | 1995-08-08 | Ee I T Kk | Machine for forming rice ball |
| US8893995B2 (en) | 2011-11-08 | 2014-11-25 | Auxocell Laboratories, Inc. | Systems and methods for processing cells |
| US9993748B2 (en) | 2014-08-11 | 2018-06-12 | Auxocell Laboratories, Inc. | Centrifuge clip and method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5722900U (en) * | 1980-07-15 | 1982-02-05 |
-
1984
- 1984-08-07 JP JP16421484A patent/JPS6143981A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5722900U (en) * | 1980-07-15 | 1982-02-05 |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07203882A (en) * | 1994-01-18 | 1995-08-08 | Ee I T Kk | Machine for forming rice ball |
| US8893995B2 (en) | 2011-11-08 | 2014-11-25 | Auxocell Laboratories, Inc. | Systems and methods for processing cells |
| US9663760B2 (en) | 2011-11-08 | 2017-05-30 | Auxocell Laboratories, Inc. | Systems and methods for processing cells |
| US9993748B2 (en) | 2014-08-11 | 2018-06-12 | Auxocell Laboratories, Inc. | Centrifuge clip and method |
| US10441901B2 (en) | 2014-08-11 | 2019-10-15 | Auxocell Laboratories, Inc. | Centrifuge clip and method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6233865B2 (en) | 1987-07-23 |
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