JPS611379A - Novel fungal strain resulting from cellfusion between edible fungi: "matsutake" and "shiitake" - Google Patents
Novel fungal strain resulting from cellfusion between edible fungi: "matsutake" and "shiitake"Info
- Publication number
- JPS611379A JPS611379A JP59120848A JP12084884A JPS611379A JP S611379 A JPS611379 A JP S611379A JP 59120848 A JP59120848 A JP 59120848A JP 12084884 A JP12084884 A JP 12084884A JP S611379 A JPS611379 A JP S611379A
- Authority
- JP
- Japan
- Prior art keywords
- shiitake
- matsutake
- mushroom
- cell fusion
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
- C12N15/04—Fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Molecular Biology (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明はマツタケとシイタケの細胞融合を用いて得らn
る新菌種に関するものである。[Detailed Description of the Invention] [Industrial Field of Application] The present invention is directed to the use of matsutake mushroom and shiitake mushroom cell fusion.
This study concerns new bacterial species.
〔従来の技術及び発明が解決しようとする問題点〕マツ
タケは古来、その香り、味が珍重さnてき友が、菌根菌
であるため、その栽培は不可能とさnて来たのに対し、
シイタケは木材腐釘菌の性質が利用され、その商業的栽
培が行なわn現在では周知の日常的食料となっている。[Problems to be solved by conventional techniques and inventions] Since ancient times, matsutake mushrooms have been prized for their aroma and taste, but because they are mycorrhizal fungi, it has been considered impossible to cultivate them. On the other hand,
Shiitake mushrooms have been cultivated commercially by utilizing the properties of wood-rotting fungi, and are now a well-known daily food.
本発明者らは、との両画類の性質を相補的に併わせもつ
新菌種を得るために、細胞融合の手法に着目し、鋭意研
究を重ねた結果、前記の性質を併有する新菌種の育種に
成功し本発明を完成するに至った。The present inventors focused on cell fusion techniques and conducted extensive research in order to obtain a new bacterial species that has the properties of both classes in a complementary manner. They succeeded in breeding the fungal species and completed the present invention.
すなわち本発明はマツタケとシイタケの細胞融合により
雑種細胞を得、こnを培養することにより得ら几る新菌
種であって、マツタケの香り、形態と、木材腐打菌の性
質を併有する新菌種を提供するものである。以下、本発
明を更に具体的に説明する。That is, the present invention is a new fungal species obtained by obtaining hybrid cells by cell fusion of matsutake and shiitake and culturing them, which has both the aroma and morphology of matsutake and the properties of wood rot fungi. It provides new bacterial species. The present invention will be explained in more detail below.
(笑施例)
まず、マツタケ、シイタケの子実体の組織片を各々表1
の組成よりなる培地に無菌的に植え込み、菌糸を生育さ
せる。(Example) First, tissue pieces of the fruiting bodies of Matsutake and Shiitake were prepared in Table 1.
The mycelium is grown aseptically in a medium with the following composition.
この菌糸を増殖させた後、各々先述の組成の液体培地に
移しかえ、継代培養の後、植え継ぎ後、約2週間の菌糸
を各々、表2.3の組成の酵素液で25℃3時間処理す
ることにより各々両画類のプロトプラストが分離される
。このプロトプラストを含む酵素液を 700XPで遠
心分離し、上澄を捨てる。After growing these hyphae, they were each transferred to a liquid medium with the composition described above, and after subculturing, the hyphae, which had been about 2 weeks old after subculturing, were treated with an enzyme solution having the composition shown in Table 2.3 at 25°C. By time processing, protoplasts of both classes are separated. Centrifuge the enzyme solution containing protoplasts at 700XP and discard the supernatant.
残渣に0.7 M Nacj’ 水溶′feLを加えて
再懸濁し、上記条件にて遠心分離を行ない上澄を捨てる
。この洗浄操作を3回くり返した後、各々1.0Mマン
ニトール水溶液にグロトプラスト濃度が1〜5XlO’
個/dKなるように再懸濁する。Add 0.7 M Nacj'feL in water to resuspend the residue, centrifuge under the above conditions, and discard the supernatant. After repeating this washing operation three times, the grotoplast concentration was 1 to 5XlO' in each 1.0M mannitol aqueous solution.
Resuspend to a concentration of 100 μg/dK.
このようにして調製したマツタケ、シイタケの両懸濁液
を混合し、70(IXF で遠心分離し、上澄を捨て、
残漬を30℃に保った表4の組成のポリエチレングリコ
ール溶液に再懸濁し10分間放置する。このS濁液に表
5の組成の融合細胞培養培地を十分量加え、700 X
Fで5分間遠心し、上置を捨てる。Both matsutake and shiitake suspensions prepared in this way were mixed, centrifuged at 70°C (IXF), and the supernatant was discarded.
The residue was resuspended in a polyethylene glycol solution having the composition shown in Table 4 kept at 30°C and left for 10 minutes. Add a sufficient amount of fused cell culture medium with the composition shown in Table 5 to this S suspension, and incubate at 700X.
Centrifuge at F for 5 minutes and discard the supernatant.
残漬を同様の操作により2度洗浄し几後、融合細胞培養
培地に再懸濁し、シャーレ上に移し、得られた融合細胞
をマイクロマニュプレーターにてピックアラブレ、融合
細胞用培地で25℃暗黒下にて培養する。培養約1ケ月
後にこの雑種細胞は***をおこない、菌糸の生長とコロ
ニー形成が観原さnt−Q
表1
(効果)
上記のようにして得らnた本発明の新菌種は増殖、徨ゴ
マ作裂、植菌、子実体形成等の過程を経て、マツタケの
香りと形態を有するキノコが生産される。しかも本発明
の新菌種はシイタケと同様木材屑A5菌の性質を有する
ので、商業的栽培が可能で69、その効果は極めて大き
い。The remaining cells were washed twice in the same manner, and after cooling, they were resuspended in a fused cell culture medium and transferred onto a Petri dish. The obtained fused cells were picked and washed with a micromanipulator and incubated in the dark at 25°C in a fused cell culture medium. Culture at the bottom. After about one month of culture, the hybrid cells divide, and hyphal growth and colony formation are observed. Through processes such as sesame seed splitting, inoculation, and fruiting body formation, mushrooms with the aroma and shape of matsutake are produced. Moreover, since the new fungal species of the present invention has the properties of wood waste A5 fungi like shiitake mushrooms, it can be cultivated commercially69, and its effects are extremely large.
第1図はマツタケのグロトプラストの顕微鏡写真、第2
図はシイタケのグロトプラストのkE84倣境写真、第
3図はマツタケシイタケ両グロトプラストの融合細胞の
顕微鏡写真である0図面1.″)浄書(内容に変更、z
・シ)ミ
;
し、
;ミ
へ
゛≧
手続補正書く方式)
1.事件の表示
昭和59年特許願 第120848号
2、発明の名称
マツタケとシイタケの細胞融合による新菌種3、補正を
する者
(事件との関係) 特許出願人
住 所 大阪府大阪市大淀区豊崎3丁目21番3号4
、補正命令の日付(発送困
昭和59年9月25日
5、補正の対象
第1図は、マツタケのプロトプラストを示す顕微鏡写真
(1750倍)でらる0
第2図は、シイタケのプロトプラストを示す顕微鏡写真
(1750倍)であるっ
第3図は、マツタケ シイタケ両プロトプラストの融合
細胞を示す顕微鏡写真(35嘩)である、尚、第1〜第
3図は、生物の形態に関する図面に代る顕微鏡写真であ
る。Figure 1 is a microscopic photograph of the glotoplast of Matsutake, Figure 2
The figure is a kE84 imitation photograph of a grotoplast of a shiitake mushroom, and Figure 3 is a micrograph of a fused cell of both glotoplasts of a shiitake mushroom. ″) engraving (change to content, z
・Sh)Mi; Shi, ;Mihe゛≧ Procedure amendment writing method) 1. Indication of the case: 1982 Patent Application No. 120848 2, Name of the invention: New bacterial species resulting from cell fusion of matsutake and shiitake mushrooms 3, Person making the amendment (relationship with the case) Patent applicant address: Toyosaki, Oyodo-ku, Osaka-shi, Osaka Prefecture 3-21-3-4
, date of amendment order (shipped September 25, 1982), subject of amendment Figure 1 is a micrograph (1750x) showing protoplasts of matsutake mushrooms Figure 2 shows protoplasts of shiitake mushrooms Figure 3 is a micrograph (1750x) showing a fused cell of Matsutake and Shiitake protoplasts. Figures 1 to 3 are diagrams showing the morphology of the organism. This is a microscopic photograph.
Claims (1)
れを培養することにより得られた新菌種A new bacterial species obtained by culturing various bacterial cells obtained by cell fusion of matsutake and shiitake
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP59120848A JPS611379A (en) | 1984-06-14 | 1984-06-14 | Novel fungal strain resulting from cellfusion between edible fungi: "matsutake" and "shiitake" |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP59120848A JPS611379A (en) | 1984-06-14 | 1984-06-14 | Novel fungal strain resulting from cellfusion between edible fungi: "matsutake" and "shiitake" |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS611379A true JPS611379A (en) | 1986-01-07 |
JPS6312591B2 JPS6312591B2 (en) | 1988-03-19 |
Family
ID=14796443
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP59120848A Granted JPS611379A (en) | 1984-06-14 | 1984-06-14 | Novel fungal strain resulting from cellfusion between edible fungi: "matsutake" and "shiitake" |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS611379A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009255290A (en) * | 2007-09-11 | 2009-11-05 | Uryu Seisaku Ltd | Impact torque adjustment device of hydraulic torque wrench |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS53130481A (en) * | 1977-04-20 | 1978-11-14 | Susumu Takegawa | Fostered mushroom by cell improvement |
JPS5729229A (en) * | 1980-07-29 | 1982-02-17 | Topy Ind | Growing of "matsutake" strain |
JPS58170473A (en) * | 1982-03-30 | 1983-10-07 | Pentel Kk | Medium for regenerating protoplast of basidiomycete |
-
1984
- 1984-06-14 JP JP59120848A patent/JPS611379A/en active Granted
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS53130481A (en) * | 1977-04-20 | 1978-11-14 | Susumu Takegawa | Fostered mushroom by cell improvement |
JPS5729229A (en) * | 1980-07-29 | 1982-02-17 | Topy Ind | Growing of "matsutake" strain |
JPS58170473A (en) * | 1982-03-30 | 1983-10-07 | Pentel Kk | Medium for regenerating protoplast of basidiomycete |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009255290A (en) * | 2007-09-11 | 2009-11-05 | Uryu Seisaku Ltd | Impact torque adjustment device of hydraulic torque wrench |
Also Published As
Publication number | Publication date |
---|---|
JPS6312591B2 (en) | 1988-03-19 |
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