JPS60149321A - Culture of mushrooms - Google Patents

Abstract

(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

【発明の詳細な説明】 本発明はきのこ類の栽培方法に関し、培養日数の短縮、
収量の増大、品質の向上などの改善が達成でき、更には
、優れたダイエタリーファイバー（食物繊維）、健康飲
料゛、薬用ドリンクスなどの提供にも有用なきのこ類の
栽培方法に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for cultivating mushrooms, reducing the number of cultivation days,
The present invention relates to a method for cultivating mushrooms that can achieve improvements such as increased yield and improved quality, and is also useful for providing excellent dietary fiber, health drinks, medicinal drinks, etc.

更に詳しくは、４本、発明は、きのこ類の人工栽培に際
し、イネ科植物の緑葉の青汁搾汁粕を培養基材としてな
る培養基において人工栽培することを特徴とするきのこ
類の栽培方法に関する。More specifically, the fourth invention relates to a method for cultivating mushrooms, which method comprises artificially cultivating mushrooms in a culture medium using green juice lees from green leaves of a grass family plant as a culture substrate. .

更に又１、本発明は、きのこ類の人工栽培に際し、イ・
ネ科植物の緑葉の青汁搾汁粕を培養基材としてなる培養
基において人工栽培し、菌糸が該培養基に蔓延また栽培
生成−物を、自己消化処理することを特徴とする優れた
ダイ５エタリーフアイバー（ｄｉ−ｅｔａｒｙ　ｆｉｂ
ｅｒｓ）、健康飲料、薬用ドリンクスなどの提供にも有
用外きのこ類の栽培方法にも関する。Furthermore, 1, the present invention provides for the artificial cultivation of mushrooms.
An excellent diet that is characterized by artificially cultivating the green juice lees of green leaves of a Neaceae plant in a culture medium as a culture substrate, allowing mycelium to spread over the culture medium and subjecting the cultivation product to autolysis. leaf iver (di-etary fib)
The present invention also relates to methods for cultivating mushrooms useful for providing health drinks, medicinal drinks, etc.

本発明は又、」二記載培方法の実施に用いるのに適した
きのこ類の苗床人工栽培用培養基材にも門する。The present invention also relates to culture substrates for the artificial cultivation of mushroom nurseries suitable for use in carrying out the culture method described in Section 2.

従来、食用もしくは薬用きのこ類の人工栽培に際して、
原木を利用する“はた水栽培法９のほかに、例えば鋸屑
を主体としこれに米糠、ふすま等の栄養源を配合した培
養基を用いる“苗床人工栽培法゛などが知られており、
工業的規模でのきのこ仙の人工栽培が可能となっている
。Conventionally, when artificially cultivating edible or medicinal mushrooms,
In addition to the hydroponic cultivation method9, which uses logs, there are also known techniques such as the artificial seedbed cultivation method, which uses a culture medium made mainly of sawdust and mixed with nutrients such as rice bran and bran.
Artificial cultivation of Mushroom Sen on an industrial scale has become possible.

しかしながら、近時、木材事情の変化から鋸屑の集荷、
入手の困難、更には、栽培するきのこ類に適した一足の
制約された品質を満足する鋸屑の羅保の困難などの課題
があシ、一方、きのこ類の人工栽培における栽培期間の
短縮、収量の増大などの改善の課題があり、これら課題
の解決のために種々の培地改良や栽培条件の改良工夫々
とについて検討がなされ、又種々な提案も行われている
。However, due to recent changes in the wood situation, the collection of sawdust
There are problems such as difficulty in obtaining sawdust, and furthermore, difficulty in obtaining sawdust that satisfies the limited quality suitable for the mushrooms being cultivated.On the other hand, shortening the cultivation period and increasing the yield in artificial cultivation of mushrooms There are issues to improve, such as an increase in the amount of nutrient, and in order to solve these issues, various improvements to culture media and cultivation conditions have been studied, and various proposals have been made.

例えば、鋸屑に代えてもしくはその一部に代えて、もみ
殻、バカス（さとうきび搾汁粕）、ビート粕（さとうだ
いこん搾汁粕）２、堅果の種皮、桑の繊維組織の処理物
、海藻などを用いたシ、或は又、米糠に代えてもしくは
その一部に代えて、大豆粕、ビール粕、雑穀類粉末、と
うもろこし胚芽、小麦胚芽、醤油粕などを用いたシする
提案が知られている（特公昭５１−３５６１３号、特公
昭５３−１０１２７号、特公昭５２−８６２号、特開昭
５３−６９８５２号、特開昭５５−７４７２４号、特開
昭５８−１６６１３号、特開昭５８−８６０１７号、な
ど）。For example, in place of sawdust or a part thereof, rice husks, bakasu (sugar cane juice residue), beet lees (sugar daikon juice residue)2, seed coats of nuts, processed products of mulberry fiber tissue, seaweed, etc. There are also known proposals to use soybean flour, beer lees, millet powder, corn germ, wheat germ, soy sauce lees, etc. in place of or in place of rice bran. (Japanese Patent Publication No. 51-35613, Japanese Patent Publication No. 10127-1980, Japanese Patent Publication No. 52-862, Japanese Patent Publication No. 69852-1982, Japanese Patent Application Publication No. 74724-1987, Japanese Patent Publication No. 16613-1980, Japanese Patent Publication No. 1982-16613, No. 58-86017, etc.).

しかしながら、これら提案の方法においても、栽培きの
こ類の種類に制約があったり、特定の複数種培養左利及
び／又は栄養源の組み合わせが必須であったり、更に、
これら適用ｉｉＱ囲もしくは適用態様の制約のほかに、
収量、栽培日数、品剥などの全ての点で満足し得るもの
は、実際上、見当らないのが実情である。However, even in these proposed methods, there are restrictions on the types of mushrooms to be cultivated, certain combinations of cultivation of multiple species and/or nutritional sources are required, and furthermore,
In addition to these restrictions on application iiQ or application mode,
The reality is that there is practically no plant that can satisfy all aspects such as yield, number of cultivation days, and product peeling.

又更に、このようなきのこ類の人工栽培の手法を利用し
て、Ａ１［たけを固体培地で人工栽培し、菌糸を」曽殖
させ、完全には子実体を形成せしめることなしに自己消
化を行わせ、分離する自己消化液部を採取する椎だけの
菌糸体の自己消化分解液の製法（特開昭５３−７５３５
２号）や茸培養固体に可食茸菌糸ケ蔓延させた後、これ
を切断・粉砕する低カロリー可食粉末の製法（特開昭５
７−２０６３６１号）のようなきのこ類の人工栽培手法
をオＵ用した他の提案も知られている。Furthermore, by using this method of artificially cultivating mushrooms, A1 [bamboo shoots were artificially cultivated on a solid medium, and the mycelia were propagated, allowing self-digestion to occur without completely forming fruiting bodies. Method for producing autolytic decomposition liquid from mycelium of only vertebrae (Japanese Patent Application Laid-Open No. 53-7535)
No. 2) or a method for producing a low-calorie edible powder by spreading edible mushroom mycelium on a mushroom culture solid and then cutting and pulverizing it (Japanese Patent Laid-Open No. 5
Other proposals using artificial cultivation techniques for mushrooms, such as No. 7-206361), are also known.

本発明者等は、きのこ類の人工栽培とくに菌床人工栽培
法の改善に関して研究を行ってきた。The present inventors have conducted research on improving the artificial cultivation of mushrooms, particularly on the artificial cultivation of fungal beds.

その結果、イネ利４（ｊｔ　？１％　とくには稲頌及び
麦類よシなる群からえらばれたイネ科植牧Ｉ、の緑葉（
茎及び葉等の地上部緑色本体を総イン」、する）の有針
搾汁粕は、それ自身単独でも、きのこ類の人工栽培にお
ける培養基材として卓越しだ適性を有する材料であって
、例えば、後に鋸屑と米１％１ｉを配合した従来慣用の
培養基材を用いた従来培養基を使用し７た場合と対比し
て実験的に示すように、顕著に改善された培養日数の短
縮、収量の増大、品質の向上を可能とする予想外且つ駕
くべき適性を壱するきのこ類の培養基材であることを発
見した。As a result, the green leaves of rice cultivation 4 (jt ? 1%), especially the grass I, selected from a group such as rice and wheat (
The needle-squeezed juice lees containing all green above-ground parts such as stems and leaves is a material that has excellent suitability as a culture substrate for the artificial cultivation of mushrooms, even when used alone. For example, as shown experimentally in comparison with a conventional culture medium containing sawdust and rice 1% 1i, the shortening of the number of culture days was significantly improved. It has been discovered that it is a culture substrate for mushrooms that has unexpected and worthy properties that enable increased yield and improved quality.

更に又、上記搾汁粕を培養暴利としてなる培養基におい
てきのこ類を人工栽培し菌糸を蔓延させ、あるいは菌糸
が該培養基に蔓延した栽培生成物を自己消化処理に賦す
る態様で、きのこ類の人工栽培を行うことによって、該
自己消化処理後の可溶性成分を取得し優れた品質の健康
飲料、薬用ドリンクスなどとして有用な生産物を得るこ
とができ、且つ又、可溶性成分取得後の固相部として優
れた品質のダイエタリーファイバーを得ることができる
ことを知った。又更に菌糸体を蔓延せしめた培養基、或
いはこれを自己消化に賦した培養基はこれを乾燥粉イｙ
ｔ＝すれば一層優れたダイエタリーファイバが得られる
ことを知った。又更に、上記イネ科植、物の緑葉の６汁
搾汁粕は、栽培するきのこ類の棹類による制約なしに、
人工栽培可能カ広い食用乃至薬用きのこ類の菌床人工栽
培用培養基材として（（「もめて有用な材料であること
を知った。Furthermore, mushrooms can be artificially cultivated in a culture medium made from the squeezed lees to allow mycelia to spread therein, or the cultivation product in which mycelium has spread in the culture medium is subjected to autolysis treatment. By carrying out cultivation, it is possible to obtain the soluble components after the autolysis treatment and obtain products useful as excellent quality health drinks, medicinal drinks, etc., and also to obtain the solid phase part after obtaining the soluble components. I learned that it is possible to obtain dietary fiber of excellent quality. Furthermore, a culture medium in which mycelium has been spread, or a culture medium which has been subjected to autolysis, can be made into a dry powder.
It was found that an even better dietary fiber could be obtained if t=. Furthermore, the above-mentioned 6-juice lees of the green leaves of the grass family plant can be used without any restrictions depending on the type of mushrooms to be cultivated.
As a culture substrate for the artificial cultivation of a wide variety of edible and medicinal mushrooms that can be artificially cultivated ("I learned that it is a very useful material.

梃って、本発明の目的は、優れた改善効果の達成できる
きのこ類の栽培方法を提供するにある。Therefore, an object of the present invention is to provide a method for cultivating mushrooms that can achieve excellent improvement effects.

本発明の他の目的は、“上記栽培方法の実施に利用する
のに適したきのこ類の菌床人工栽培用培養基材を提供す
るにある。Another object of the present invention is to provide a culture substrate for artificial cultivation of mushroom beds suitable for use in carrying out the above cultivation method.

本発明の上記目的及び更に多くの他の目的ならびに利点
は、以下の記載から一層明らかとなるでおろう。The above objects and many other objects and advantages of the present invention will become more apparent from the following description.

本発明における培養基は、イネ科植物の緑葉の青汁搾汁
粕から成る。好捷しくは、イネ科植物の成熟期（緑葉が
実質的に黄変する時期）前の緑部の搾汁処理した粕が利
用され、より好捷しくは穂揃期及び穂揃期前、更に好捷
しくは出穂開始期及び出穂開始期前、とくに好ましくは
分法開始期乃至出穂開始期前の緑葉の搾汁処理した粕が
利用できる。The culture medium in the present invention consists of green juice lees of green leaves of a grass family plant. Preferably, the lees obtained by squeezing the green part of the grass before the maturity stage (the time when the green leaves substantially turn yellow) are used, and more preferably, during the panicle stage and before the panicle stage, More preferably, lees obtained by squeezing green leaves at the beginning of heading and before the beginning of heading, particularly preferably at the beginning of dividing or before the beginning of heading, can be used.

イネ科植物の好寸しい例としでは、揺角及び麦類よりな
る群からえらはれたイネ科植物を挙けることができ、そ
の具体例としては、各種の陸稲ならびに水稲、大麦、裸
麦、えん麦、イタリアン・ダイグラスなどを例示するこ
とができる。Suitable examples of grasses include those selected from the group consisting of rockweed and wheat; specific examples include various types of upland rice, paddy rice, barley, naked wheat, Examples include oats and Italian diced grass.

イネ科植物の緑葉の青汁搾汁粕は、たとえば、該緑葉を
そのまま或は適当に細切したり破砕処理して、例えはロ
ール圧搾タイプ、ミキサータイプ、ジューサータイプ、
その他所望のタイプの搾汁伏を用いて４ｙｓ汁し一汗汁
かし分力ＩＬすることにより得ることができる。The green juice lees of green leaves of gramineous plants can be obtained, for example, by using the green leaves as they are, or by appropriately cutting or crushing them into pieces, such as roll pressing type, mixer type, juicer type, etc.
It can also be obtained by squeezing 4ys of juice using any other desired type of juice strainer and straining it for one minute.

このようにして得ることのできるイネ旧７１１ＩＩ物の
緑葉の合計搾汁粕は、ぞの−ままの形態で利用すること
ができるし、或は父、／ことえば切断処理、破砕処理、
粉末化処理などの所望の処理を施して、切ｕＪｒ’物、
粗砕物、粉末などの形態にしで利用するとともてきる。The juice residue of the green leaves of the old rice plant 711II that can be obtained in this way can be used in its original form, or it can be used in its original form, such as by cutting, crushing, etc.
After applying desired treatment such as powdering treatment, cut uJr' products,
It can be used in the form of crushed products or powder.

史に又、ノシ［望により、乾燥処理や冷凍処理に賦して
乾燥物や玲凍物などのル態にして′Ａ：す用することも
できる。イネ利稙、を吻とくには麦類の緑葉の青汁（ｄ
：惚めで有用であって、食用植物の一内汁さらには友矢
１′ｉの緑葉漬汁の安定な粉末の製造に１’、Ｉ　して
にＬｌ例えば府会１＋１４４６　４１１７７号、特公昭
４６−３８５４８号などに開示されており、本発明でオ
リ用するイネ旧植物の緑葉の＾゛汁搾汁粕としては、こ
れらに開示されたイネ旧植物の緑葉の青汁を採取した残
部の搾汁粕を利用することもできる。Additionally, if desired, it can be used in the form of dried or frozen products by subjecting it to drying or freezing treatment. Inaritane, especially the green juice of the green leaves of wheat (d
1', I and Ll are useful for producing stable powders of edible plants such as Ichiuchi juice and even green leaf pickled juice of Tomoya 1'i. -38548, etc., and the juice residue of green leaves of old rice plants used in the present invention is the squeezed residue of the green juice of green leaves of old rice plants disclosed in these documents. You can also use soup lees.

イネ科植物の緑葉の青汁搾汁粕、好首しくはイネ科植物
の成熟期前の緑葉の搾汁処理した粕を有効成分とするき
のこ類の菌床人工栽培用培養基材が、顕著に改善された
培養日数の短縮、収量の増大、品質の向上を可能とする
予想外且つ篤くべき適性を示すきのこ類の培養左利とな
る理由の詳細は不明であるが、イネ科植物の緑葉の青汁
搾汁粕は生育途上の若い柔軟な繊維質が破砕された状態
になっていて、極めて多孔性に富み、柔軟性で且つ弾力
性に富んでおり、優れた通気性、吸水性、保水性を有す
るスポンジ様物性の構造体であること、更には、該搾汁
粕の主成分であるイネ科植物（０８葉由来のセルロース
、ヘミセルロース、リクニンなどの細胞膜構成成分は成
熟した細胞膜構成成分に比して、きのこ類の菌糸体の生
育増殖に資化され易い形態に容易に酵素などにより分解
、変換される傾向があること、又更に、該搾汁粕中には
イネ科植物緑葉の旺盛な生長期細胞物質の組成物、たと
えば比較的低分子の蛋白質、ペプチド類、アミノ酸類な
どの窒素源、オリゴ糖その他各種の炭水化物などの炭素
源、さらに各種ビタミン類、ミネラル類が分解、吸収さ
れ易い状態及びバランスで含有され、斯くてきのこ類の
菌糸体の生育増殖に好適な窒素源、炭素源、ビタミン類
、ミネラ＼ ル類の供給源となること、等が因子となっているものと
推測しているが、後に従来慣用の苗床人工栽培用培養基
材を用いた場合と比較して、実験的に示すように、該搾
汁粕単独でも顕著に改善された培養日数の短縮、収量の
増大、品質の向上を可能とする。A culture substrate for artificial cultivation of mushroom beds containing as an active ingredient lees from green juice squeezed from green leaves of grasses, preferably from green leaves of grasses before the maturation stage, is remarkable. Although the details of why mushrooms are advantageous for culturing mushrooms, which show an unexpected and remarkable aptitude for shortening the cultivation period, increasing yield, and improving quality, are unknown, The green juice lees are crushed young flexible fibers that are still growing, and are extremely porous, flexible, and elastic, with excellent breathability, water absorption, It is a structure with sponge-like physical properties that has water-retaining properties, and furthermore, cell membrane constituents such as cellulose, hemicellulose, and likunin derived from grasses (08 leaves), which are the main components of the squeezed lees, are mature cell membrane constituents. In contrast, it tends to be easily decomposed and converted by enzymes into a form that is easily assimilated by the growth and proliferation of mushroom mycelium. Composition of vigorous cell material during growth, such as nitrogen sources such as relatively low-molecular proteins, peptides, and amino acids, carbon sources such as oligosaccharides and various carbohydrates, and various vitamins and minerals are decomposed and absorbed. Factors such as being contained in a state and balance that makes it easy for mushrooms to grow, and thus serving as a source of nitrogen, carbon, vitamins, and minerals that are suitable for the growth and proliferation of mushroom mycelia. However, as shown experimentally, compared to the case of using culture substrates for conventional nursery artificial cultivation, using the juice lees alone significantly improved the shortening of the number of cultivation days. Enables increased yield and improved quality.

本発明で利用するイネ科植物の緑葉の青汁搾汁粕の一例
として、大麦若葉緑葉（葉及び茎；草丈２５〜３５　ｃ
ｒｒＬ）の青汁搾汁粕の組成の一例を下掲給１表に示す
。As an example of the green juice lees of green leaves of gramineous plants used in the present invention, young barley green leaves (leaves and stems; plant height 25 to 35 cm) are used in the present invention.
An example of the composition of the green juice lees of rrL) is shown in Table 1 below.

本発明方法によれば、上述の如きイネ科植物の緑葉の青
汁搾汁粕、好ましくはイイ・科植物の成熟期前の緑葉の
搾汁処理した粕を培養基材としてなる培養基において、
きのこ類を人工栽培する。人工栽培の手法それ自体は知
られており、本発明方法で利用でき、菌床人工栽培する
きのこ類の種類、栽培目的、用途などに応じて適当に選
択実施できる。例えは、びん床栽培方式、製法栽培方式
、箱栽培方式、その他公知の任意の人工栽培方式を利用
することができる。基本的な手法としては、培養基材の
水分調製、充床、殺菌、冷却、種菌接種、培養、菌掻き
、芽出しなどの操作を適宜に行ない、きのこ類菌糸体の
生育、生長、蔓延、熟成、子実体の育成などを、それぞ
れの菌種や目的に応じて湿度、含水率、温度、光度など
を調節し行なうことによシ、きのこ類の人工栽培を実施
することができる。According to the method of the present invention, in a culture medium made of the above-mentioned green juice lees of green leaves of a plant belonging to the Poaceae family, preferably lees obtained by squeezing green leaves before the maturity stage of a plant belonging to the Poaceae family, as a culture substrate,
Artificial cultivation of mushrooms. Artificial cultivation methods themselves are known and can be used in the method of the present invention, and can be appropriately selected and implemented depending on the type of mushrooms to be artificially cultivated in a fungal bed, the purpose of cultivation, the use, etc. For example, a bottle bed cultivation method, a manufacturing method cultivation method, a box cultivation method, or any other known artificial cultivation method can be used. The basic method is to appropriately perform operations such as moisture preparation of the culture substrate, filling, sterilization, cooling, inoculum inoculation, culturing, scraping, and sprouting to grow, grow, spread, and ripen the mushroom mycelium. Artificial cultivation of mushrooms can be carried out by adjusting the humidity, water content, temperature, light intensity, etc. according to the species and purpose of each mushroom.

本発明においては、前述したように、イネ科植物の緑葉
の青汁搾汁粕の単独使用によって、きのこ類の人工栽培
を行なうことができるが、所望により、各種の補助材を
適宜に選択して併用することができる。このような補助
材の例としては、たとえば鋸ハｔ１籾殻、米糠、ふすま
、バヵス、ビート粕、とうもろこし粕、ビール粕、酵母
エキス、各第１Ｉアミノ酸、カザミノ酸、硫酸塩類、燐
酸塩類、チアミン、リボフラビン、Ｌ−アスコルビン酸
、ニコチン酸、ビオチン、ビタミンＢ６などを例示する
ことができる。In the present invention, as described above, mushrooms can be artificially cultivated by solely using the green juice lees of green leaves of gramineous plants, but if desired, various auxiliary materials may be appropriately selected. Can be used together. Examples of such auxiliary materials include sawtooth rice husks, rice bran, bran, bacchus, beet lees, corn lees, beer lees, yeast extract, I-I amino acids, casamino acids, sulfates, phosphates, thiamin, Examples include riboflavin, L-ascorbic acid, nicotinic acid, biotin, and vitamin B6.

本発明においては、人工栽培可能な広い食用乃至薬用き
のこ類の菌床人工栽培を行うことができ、たとえば椎茸
、ナメコ、シメジ、エノキダケ、ヒシタケ、キクラゲ、
その他の食用きのこ類、たとえば霊芝（マンネンダケ）
、カワラタケ、イに苓、猪苓、雷丸その他の主として薬
用に供する薬用きのこ類、その他各種のきのこ類の人工
栽培を行うことができる。In the present invention, it is possible to artificially cultivate a wide variety of edible and medicinal mushrooms, such as shiitake mushrooms, nameko mushrooms, shimeji mushrooms, enoki mushrooms, water chestnut mushrooms, wood ear mushrooms,
Other edible mushrooms, such as Ganoderma
It is possible to artificially cultivate medicinal mushrooms mainly used for medicinal purposes, such as Kawaratake, Inirei, Irei, Raimaru, and other various mushrooms.

本発明によれば、子実体の採取を目的としたきのこ類の
人工栽培のほかに、ダイエタリーファイバー、健康飲料
、薬用ドリンクスなどの提供を目的として、きのこ類の
人工栽培に際し、イネ科植物の緑葉の青汁搾汁粕を培養
基材としてなる培養基において人工栽培し、菌糸が該培
養基に蔓延した栽培生成物を自己消化処理することを特
徴とするきのこ類の栽培方法も提供できる。According to the present invention, in addition to the artificial cultivation of mushrooms for the purpose of collecting fruiting bodies, when artificially cultivating mushrooms for the purpose of providing dietary fiber, health drinks, medicinal drinks, etc. It is also possible to provide a method for cultivating mushrooms, which comprises artificially cultivating the green juice lees of green leaves in a culture medium serving as a culture substrate, and subjecting the cultivation product in which mycelia have spread to the culture medium to autolysis.

この態様によれば、自己消化処理後可溶化された成分を
液相部として侵れた品質の健康飲料、薬用ドリンクス成
分が採取でき、又、自己消化処理後培養基ごと、あるい
は上記液相部取得残部を固相部として極めて口当りのよ
い滑かで且つ軟質のダイエタリーファイバーを得ること
ができる。例えば、薬用きのこ類の上記態様による人工
栽培を行ない優れた健康飲料乃至薬用ドリンクスを提供
できる。この際、適尚な添加物を配合して最終製品とす
ることができ、この上うな添力１」物の例として、例え
ば、醸造酢類、クエン酸、酒石酸などのｏｆ食性翁椴敵
、リジン、アスパ、ラギン酸などのアミノ配、ブドウ糖
、麦芽糖、蜂蜜などの糖類、ソルビット、マンニットな
どの糖アルコール類、ステビア、アスパルテームなどの
甘味料類、ビタミンＪ３．　、Ｂ２．Ｃ”などビタミン
類、カルシウム、鉄などミネラル等、更には斗旬４己、
人参、コンフリーなど保健植物のエキス類、クアニラな
ど食用香料類、共用色素、醗造酒精、酒類などを例示す
ることができる。又、菌糸を蔓延させた培養基、あるい
はそれを自己消化処理したものおよび自己消化処理固相
部は、たとえば乾燥粉砕して、ダイエタリーファイバー
として利用できる。例えば、大麦若葉の緑葉（草丈約２
５ｃｍ茎葉共）の青汁搾汁粕を水分７０％に調節して培
養基となし、ポリプロピレン袋に包み常法により加圧蒸
気殺ｌし、これにマンネンタケ輝−を接種して２７〜２
８℃の温度条件に保持した培養室内で約５０日間培養し
、菌糸が培養基全体に亨延し児熟状態になったとき培養
基を取シ出し粗く粉砕したのち、′ｐｕ約５０に調節し
た水を培養基と略等重量力１」え、ポリプロピレンに包
み４０〜５０℃に４〜５時ＩＦＡＪ保持し菌糸体の自己
消化を促進せし′める、培養基を加圧沢過し、涙液を採
取し、洗浄液と併わせ保健ドリンク剤、薬用ドリンク剤
の原液とする。このものはマンネンタケ繭糸体の有効成
分’ｃ金含有るとともに大麦若葉の青汁の有用成分を含
有する極めてユニークなものである。又、麿、液採取後
の残存物は大麦若葉の繊維質が一層柔軟になり、また一
部の消化も起り、リグニン質の分解などによシ一層易食
性のある目障シのよいものに転換され、且菌糸体多糖類
、核酸ミネラルを含む優れたダイエタリーファイバの供
給源となる。また上記自己消化処理培養基をその１ま乾
燥、粉砕したものは一層優れたダイエタリーファイ−バ
ーとして利用することができる。上記態様において、菌
糸がイネ科植物の緑葉の青汁搾汁粕に蔓延した栽培生成
物の自己消化処理は、例えば、以下のようにして行うこ
とができる。即ち菌糸体が蔓延した培養基を、適当な手
段によシ、粉砕、攪拌などを行ない自己酵素の作用を受
け易い状態となし、所望に応じ適宜加水し、所望のｐ　
Ｅ　Ｋ整え、それぞれの作用至適温度４０〜５０°Ｃに
保持し自己消化を行わせる。According to this aspect, it is possible to collect the components of healthy drinks and medicinal drinks with the components solubilized after the autolysis treatment as a liquid phase part, and also to collect the whole culture medium or the liquid phase part after the autolysis treatment. Using the obtained residue as a solid phase, a very palatable, smooth and soft dietary fiber can be obtained. For example, excellent health drinks or medicinal drinks can be provided by artificially cultivating medicinal mushrooms according to the above embodiments. At this time, suitable additives can be added to the final product. Examples of additives include fermented vinegar, citric acid, tartaric acid, etc. Amino compounds such as lysine, aspar, and lagic acid, sugars such as glucose, maltose, and honey, sugar alcohols such as sorbitol and mannitol, sweeteners such as stevia and aspartame, and vitamin J3. , B2. Vitamins such as "C", minerals such as calcium, iron, etc., as well as Doushun 4ki,
Examples include extracts of health plants such as carrot and comfrey, edible flavorings such as quanilla, common colorants, distilled spirits, and alcoholic beverages. In addition, a culture medium in which mycelium is spread, or a culture medium subjected to autolysis treatment, and a solid phase portion subjected to autolysis treatment can be used as dietary fiber, for example, by drying and pulverizing the culture medium. For example, green leaves of young barley (plant height approx.
A culture medium was prepared by adjusting the moisture content to 70%, and wrapping it in a polypropylene bag and sterilizing it with pressure steam using a conventional method.
Cultivate for about 50 days in a culture chamber maintained at a temperature of 8°C, and when the mycelium has spread throughout the culture medium and reached a mature state, remove the culture medium and coarsely crush it. The culture medium was wrapped in polypropylene and held at 40 to 50°C for 4 to 5 hours to promote self-digestion of the mycelia. It is collected and combined with a cleaning solution to make a stock solution for health drinks and medicated drinks. This product is extremely unique as it contains gold, an active ingredient of the cocoon threads of C. chinensis, as well as useful ingredients of the green juice of young barley leaves. In addition, the fibrous material of the young barley leaves that remains after extracting the liquid becomes more flexible, and some digestion occurs, resulting in the decomposition of lignin, making it more edible and more palatable. It is an excellent source of dietary fiber, including mycelial polysaccharides, nucleic acid minerals. Furthermore, the above-mentioned autolyzed culture medium that has been dried and ground can be used as an even better dietary fiber. In the above embodiment, the autolysis treatment of the cultivated product in which hyphae are spread in the green juice lees of green leaves of a grass family plant can be performed, for example, as follows. That is, the culture medium in which mycelium is infested is made into a state where it is susceptible to the action of autoenzymes by crushing, crushing, stirring, etc. by appropriate means, and water is added as needed to achieve the desired p.
Adjust EK and maintain the optimum temperature for each action at 40-50°C to allow autolysis.

以下、実施例によシ本発明の数態様について、更に詳し
く説明する。Hereinafter, several aspects of the present invention will be explained in more detail by way of examples.

実施例１および比較例１ 大麦緑葉（葉及び茎；草丈約２５Ｇ）を搾汁機を用いて
切断圧搾して青汁（搾汁液）を採取しだ残相を乾燥して
大麦緑葉の青汁搾汁粕の乾燥物を得た。この乾燥搾汁粕
（水分含量約６φ）４５０Ｉを採り、水を加えて充分に
混和して含水率が約６４チになるように調節したのち、
とれを容量８００ｍのポリプロピレン製広口ビンに充填
し、密栓後、約１２０℃で１時間加圧蒸気殺菌を行った
。放冷後、ヒラタケ種菌を接種し、温度約１９℃、温度
約６０〜７０％の条件下の培養室内に収容して培養した
。菌糸がビン全体に蔓延繁殖したのち、菌掻きを行ない
、その後、温度１０〜１５°Ｃ１湿度約９０チに保持し
た培養室で人工栽培した。Example 1 and Comparative Example 1 Barley green leaves (leaves and stems; plant height approximately 25G) are cut and squeezed using a juice extractor to collect green juice (squeezed liquid), and the remaining phase is dried to produce green barley leaf green juice. A dried product of squeezed lees was obtained. Take 450 I of this dried juice lees (moisture content about 6 φ), add water and mix thoroughly to adjust the moisture content to about 64 in.
The pellets were filled into a polypropylene wide-mouth bottle with a capacity of 800 m, and after being tightly stoppered, pressure steam sterilization was performed at about 120° C. for 1 hour. After cooling, the Oyster mushroom inoculum was inoculated and cultured in a culture chamber at a temperature of about 19° C. and a temperature of about 60 to 70%. After the mycelia spread throughout the bottle, they were scraped and then artificially cultivated in a culture room maintained at a temperature of 10-15° C. and a humidity of about 90° C.

比較のため、鋸屑３７５ｇと米糠１０５ｇを混合し含水
率が約６４％になるように調節したほかは、上記実施例
１と同様にしてヒラタケ種菌を接種し、同様にして人工
栽培を行った（比較例１）。For comparison, oyster mushroom inoculum was inoculated in the same manner as in Example 1 above, except that 375 g of sawdust and 105 g of rice bran were mixed and the moisture content was adjusted to approximately 64%, and artificial cultivation was performed in the same manner ( Comparative example 1).

その結果は、下掲第２表に示したとおりであった。The results were as shown in Table 2 below.

なお、第２表に於て、子実体収量はビン１０個について
の平均収量である。In addition, in Table 2, the fruit body yield is the average yield for 10 bottles.

第２表 実施例１　比較例２ 培養日数（日）　１６　２４ 閉掻き以降、収穫捷での 栽培日数（日）　７　１３ 培養開始からの成育期間 総合泪（日）　２３　３７ 〔ビン当シ〕８４．８　６６．１ 子実体の品質　優秀　良 上掲第２表の結果に示されるように、本発明の培養基を
用いたｍ床人工栽培の結果は、従来慣用の培養基を用い
た結果に比して、菌糸体の繁殖が顕著に活発化され、更
に菌掻きから子実体収穫までの期間も著るしく短縮され
て、成育期間が顕著に短縮されると共に、子実体収量及
び品質においても著るしく優れた改善効果が達成できる
。Table 2 Example 1 Comparative Example 2 Number of cultivation days (days) 16 24 Number of cultivation days at harvesting point after closing (days) 7 13 Total growth period from the start of cultivation (days) 23 37 [Bottles] 84 .8 66.1 Quality of fruiting body Excellent Good As shown in the results in Table 2 above, the results of m-bed artificial cultivation using the culture medium of the present invention are better than those using conventional culture medium. As a result, the reproduction of mycelium is significantly activated, and the period from scraping to fruiting body harvesting is also significantly shortened, which significantly shortens the growth period and significantly improves fruiting body yield and quality. It is possible to achieve excellent improvement effects.

実施例２および比較例２ エンバク緑葉（葉及び茎；草丈約２５α）を、実施例１
と同様にして搾汁処理した残相の約１４００＆を採り、
水分約６４チに々るように乾燥、調節したのち、容量８
００　ｍｌのポリプロピレン製広口ビンに充填し、実施
例１と同様にして殺菌処理した。放冷後、エノキダケ種
菌を接種し、温度約１７〜１８℃、温度約７０〜８０係
に調節した間歇換気装置、付きの培養室内で菌糸体の培
養を行った。菌糸が充分に畳延繁殖したのち菌掻きを行
ない、室温約１３〜１４℃、温度約８５〜９０係に保っ
た芽出し室において芽出しを行った。Example 2 and Comparative Example 2 Oat green leaves (leaves and stems; plant height approximately 25α) were used in Example 1.
Approximately 1400% of the remaining phase was squeezed in the same manner as above,
After drying and adjusting the moisture content to about 64 cm, the capacity is 8.
The mixture was filled into a 00 ml wide-mouthed polypropylene bottle and sterilized in the same manner as in Example 1. After cooling, the Enokidake seed fungus was inoculated, and the mycelium was cultured in a culture chamber equipped with an intermittent ventilation device and adjusted to a temperature of about 17-18° C. and about 70-80°C. After the hyphae had sufficiently propagated, they were scraped and germinated in a budding chamber maintained at a room temperature of about 13-14°C and a temperature of about 85-90°C.

ついで、約４℃に維持した抑制室において冷風換気条件
下で抑制処理したのち、温度約６〜７°Ｃの条件下で生
育栽培を行った。Subsequently, the plants were subjected to suppression treatment under cold ventilation conditions in a suppression chamber maintained at about 4°C, and then grown and cultivated at a temperature of about 6 to 7°C.

比較のため、鋸屑３９５ｇと米糠９５ｇを撹拌混合し、
水を加えて含水率を約６４チになるように調節した培養
基を用いるほかは実施例２と同じ榮件下に同様に操作し
てエノキダケの人工栽培を行った（比較例２）。その結
果を下掲第３表に示した。尚、表中、子実体収量及び品
質の評価は実施例１について述べたと同じである。For comparison, 395 g of sawdust and 95 g of rice bran were stirred and mixed.
Enoki mushrooms were artificially cultivated under the same conditions as in Example 2, except that a culture medium whose water content was adjusted to about 64 cm by adding water was used (Comparative Example 2). The results are shown in Table 3 below. In the table, the evaluation of fruit body yield and quality is the same as described for Example 1.

第３表 実施例２　比較例２ 培イ卵日数（日）　２０　２３ 酌掻き以降、芽出し終了 までの芽出し日数（日）　８　１０ 抑９ｊ１」処理日数（日）　１０　１２生行日数（ロ）
　ｉｏ　１０ 培養からの成育期間総合計 （日）　４８　５５ 子実体収す（，９）　１２８　１０５ 〔ビン当シ〕 子芙体の品質　医　良 実施例３及び比較例３ 裸麦緑葉（幼穂形成開始期の葉及び茎；草丈約２０の）
を搾汁機を用いて搾汁し、青汁を採取した残相を乾燥し
たのち粉砕機で粉砕して、約４０〜６０メツシユの青汁
搾汁粕細粉を調製した。Table 3 Example 2 Comparative Example 2 Number of days for culturing eggs (days) 20 23 Number of days for sprouting from the time of stirring until the end of sprouting (days) 8 10 Number of days for suppression 9j1 treatment (days) 10 12 Number of days for survival (b)
io 10 Total growth period from culture (days) 48 55 Harvesting fruiting bodies (,9) 128 105 [Bottled] Quality of seedlings Medical Good Example 3 and Comparative Example 3 Bare wheat green leaves (starting stage of ear formation leaves and stems; plant height approximately 20 cm)
The green juice was extracted using a juice extractor, and the residual phase obtained by collecting the green juice was dried and then crushed using a pulverizer to prepare about 40 to 60 mesh green juice squeezed lees fine powder.

得られだ搾汁粕粉末に加水して含水率が約７０チとなる
ように調節し、その約４０ｇを採り、９、５　ｃｒｎ　
（直径）のシャーレにほぼ一定の高さとなるように充填
したのち、約１２０℃で４０分間加圧殺菌した。室温に
冷却後、予じめ馬鈴薯蔗糖培地で培養しておいたマンネ
ンタケ種菌を径７ｉｍのコルクポーラ−で打抜き、この
菌糸体ディスクを上記シャーレ中の培養基の中火部に接
種し、約２５℃で８日間培養したのち、菌糸体の生育度
をディスクからの菌糸の繁殖伸長半径を測定することに
よシ判定した。Add water to the obtained juice lees powder to adjust the moisture content to about 70 g, take about 40 g of it, and add 9.5 crn.
(diameter) to a nearly constant height, and then pressure sterilized at about 120° C. for 40 minutes. After cooling to room temperature, punch out the L. chinensis seed fungus, which had been previously cultured in a potato-sucrose medium, using a cork polarizer with a diameter of 7 mm, and inoculate this mycelium disk into the medium-heated part of the culture medium in the above-mentioned petri dish. After culturing for 8 days, the growth rate of the mycelium was determined by measuring the radius of propagation and elongation of the mycelium from the disk.

比較のため、鋸屑８部と米糠２部を混合しだ従来培養基
を用いるほかは実施例３と同じ条件下で同様に操作して
マンネンタケの苗圧人工栽培を行った（比較例３）。For comparison, artificial pressure-pressure cultivation of Seedling mushrooms was carried out under the same conditions as in Example 3, except that 8 parts of sawdust and 2 parts of rice bran were mixed and a conventional culture medium was used (Comparative Example 3).

その結果を下掲第４表に示した。The results are shown in Table 4 below.

量丸敷 実施例３　３５　１１ 比較例３　１７　１８ 」二掲第４表の結果に示きれるように、本発明の培養基
を用いることによって、従来慣用の培養基を用いた場合
に比して、菌糸体の繁ダαが明らかに促進されることが
わかる。Example 3 35 11 Comparative Example 3 17 18 As shown in the results in Table 4, by using the culture medium of the present invention, compared to the case where a conventional culture medium was used, mycelia It can be seen that body growth is clearly promoted.

実施例４及び比較例４ 裸麦緑葉（幼穂形成開始期の葉及び茎；草丈約２０〜３
０　ｃｍ）を搾汁機を用いて搾汁し、青汁を採取した残
相を乾燥して、裸麦緑葉の敲汁搾汁粕の乾燥物を得た。Example 4 and Comparative Example 4 Bare wheat green leaves (leaves and stems at the beginning of panicle formation; plant height approximately 20-3
0 cm) was squeezed using a juice squeezer, and the remaining phase from which the green juice was collected was dried to obtain a dried product of the squeezed lees of naked barley green leaves.

このｊ）ｌ燥搾汁粕１ｏｏｏ、ｙ（ｔｋｇ）を採り、含
水率約６５係に調節しグこのち、これをポリエチレンフ
ィルムで包み、約１２０℃で１時間高圧殺菌処理した。100, y (tkg) of this j)l dried juice lees was taken, the moisture content was adjusted to about 65%, and then it was wrapped in a polyethylene film and subjected to high-pressure sterilization at about 120° C. for 1 hour.

処理後、直りに７０×４０Ｘ１７（α）のダンボール箱
に収容し、室温まで冷却したのち、実施例３でのべたと
同様なマンネンタケ種菌１００ｇを接種した。約２７°
Ｃで２５日間培養して培養基全体に菌糸体を６〉延させ
たのち、さらに３０日間培養を続けてからポリエチレン
フィルムを開いて散水し、約２２℃で発芽、栽培処理を
行った。After the treatment, it was immediately placed in a 70 x 40 x 17 (α) cardboard box, cooled to room temperature, and then inoculated with 100 g of the same L. monocytogenes inoculum as in Example 3. Approximately 27°
After culturing in C for 25 days to spread mycelium over the entire culture medium, culturing was continued for an additional 30 days, the polyethylene film was opened, water was sprinkled, and germination and cultivation were performed at approximately 22°C.

比較のため、鋸）Ｙｔ　８００　ｇ　ト米ｔｆ）ｔ　２
００　ｇを混合し、含水率約６５チに調節した培養基を
用いるほかは、上記実施例４と同様にして栽培処理を行
った（比較例４）。For comparison, saw) Yt 800 g Tome tf) t 2
Cultivation treatment was carried out in the same manner as in Example 4 above (Comparative Example 4), except that a culture medium containing 0.00 g and adjusted to a moisture content of approximately 65 cm was used.

その結果を下掲第５表に示しだ。The results are shown in Table 5 below.

第５六 発生Ｍ（Ｅｌ　）　５２　２３ 子実体の発生個数（ケ）４２ 子実体１ケの平均重量 （＆）　１３　１１．５ 培地当りの歩留り（裂）　５．２　２．３上吊第５表の
結果に示されるように、本発明の培養基を用いることに
よって、従来慣用の培養基ケ用いた場合に比して、子実
体の発生量、発生個数、子実体１ヶ当りの平均ｖｍ及び
歩留りにおいても顕著な改善が達成できることがわかる
。56th Generation M (El) 52 23 Number of fruiting bodies (ke) 42 Average weight of one fruiting body (&) 13 11.5 Yield per medium (fissures) 5.2 2.3 Suspension Table 5 As shown in the results, by using the culture medium of the present invention, the amount of fruiting bodies produced, the number of fruiting bodies, the average vm per fruiting body, and the yield were lower than when using conventional culture media. It can be seen that a significant improvement can also be achieved.

実施例５ 大麦緑葉（幼穂形成開始期の葉茎；草丈約１５〜２５の
）を搾汁機を用いて搾汁処理し、青汁を採取した残相を
乾燥して大麦緑葉の有針搾汁粕の乾燥物を得た。この乾
燥物１ｋ１９に加水して當水率約７０係に調節したのち
、ポリプロピレンフィルムに包み、約１２０°Ｃで１．
５時１ｉｆ４加圧殺菌した。Example 5 Barley green leaves (leaves and stems at the beginning of panicle formation; plant height approximately 15 to 25 cm) are squeezed using a juice extractor, the green juice is collected, the remaining phase is dried, and barley green leaves are squeezed with needles. A dried product of soup lees was obtained. After adding water to 1k19 of this dried product to adjust the water content to about 70%, it was wrapped in polypropylene film and heated at about 120°C for 1.
It was sterilized under pressure at 1:4 at 5:00.

室温まで放冷したのちマンネンタケの種菌を植菌し、約
２７〜２８℃の温度条件に保持した培養室で５０日間培
養して、菌糸体が培地中に充分に蔓妙した完熟状態とな
ったところで、培地をポリプロピレンフィルムより取り
出して自己消化処理／りに移した。］、　Ｏｌの水を加
え、才も塩酸を用いて系のｐＥを５に調整し、攪拌しな
がら約４０°〜５０℃の温度条件で２時間の自己消化処
理に賦した。After cooling to room temperature, the inoculum of Cinnamon mushroom was inoculated and cultured for 50 days in a culture room maintained at a temperature of approximately 27 to 28 degrees Celsius, until the mycelium was fully grown in the medium and reached a fully ripe state. By the way, the culture medium was taken out from the polypropylene film and transferred to an autolysis treatment. ], water was added, the pE of the system was adjusted to 5 using hydrochloric acid, and the system was subjected to autolysis treatment for 2 hours at a temperature of about 40° to 50° C. with stirring.

得られた自己消化処理系に醸造米酢３０ｍｃｌを添加し
約１時間煮沸処理したのち遠心分離して分離液を得た。30 mcl of brewed rice vinegar was added to the resulting autolysis treatment system, and the mixture was boiled for about 1 hour and then centrifuged to obtain a separated liquid.

得られた自己消化処理液を一昼夜静置したのち濾過助剤
として珪藻土を加えて濾過し濾過成約８５ノを得、これ
にグラニユー糖３００ｇ、液糖ｇ　ｏ　ｏ　ｐ、クエン
酸２０１１アスコルビン酸２（ｌ及びリンゴ酸２ｇを加
えたのち、水で全量１０１に７１）釈した。これを漣過
処理したのち約７０°Ｃで３０分間殺菌して、嗜好性の
よい霊芝菌糸体ドリンクスを調製した。The resulting autolyzed solution was allowed to stand for a day and night, and then diatomaceous earth was added as a filter aid and filtered to obtain a filtered product of about 85 g. After adding 1 g of malic acid and 2 g of malic acid, the total volume was diluted with water to a total volume of 101 g. This was subjected to a filtration treatment and then sterilized at about 70°C for 30 minutes to prepare a palatable Ganoderma mycelium drink.

遠心分離し分離液を得た残渣を乾燥機にて乾燥した後粉
砕し、なめらかな目障シのよいグイエフリーフアイバー
粉末ケ得た。The residue obtained by centrifugation was dried in a drier and pulverized to obtain a smooth Gouief Leaf Iver powder with good eyesight.

実施例６ 大麦若葉（幼穂形成開始期の業茎、草丈２０ｃａ）を用
い実施例５と同様な方法でマンネンタケ菌糸体就延完熟
状態となした培養基を粗砕機にて粗砕し、４ＹＪ３１の
ｐ　Ｈ５，０に調整した温水を加えポリエチレン袋中で
密閉し約４０〜５０℃に６時間保ち自己消化を行わしめ
た。これを乾燥粉末化しダイエツトファイバーを調製し
た。このものは大麦若葉の柔軟多孔性の繊維がさらに消
化され、リグニン質の分解もともない極めて口障りのよ
い易食性繊維質に転換され、これにマンネンタケ多糖類
、それらの酵素分解物、マンネンタケの有用生理活性成
分、大麦若葉青汁の有用成分が共存する理想的なダイエ
タリーファイバーである。このものは高脂質血症、動脈
硬化症、糖尿病、肥満、さらには腸癌などの予防に食餌
効果をもたらすものであり、免疫増強作用、抗腫瘍作用
などが期待される優れたダイエタリーファイバーである
。Example 6 Using young barley leaves (industrial stalks at the beginning of panicle formation, plant height 20 ca), the culture medium was grown to a fully ripe state with C. aureus mycelium in the same manner as in Example 5, and the culture medium was coarsely crushed using a crusher to obtain 4YJ31 p. Hot water adjusted to H5.0 was added, and the bag was sealed in a polyethylene bag and kept at about 40 to 50°C for 6 hours to allow autolysis. This was dried and powdered to prepare dietary fiber. In this product, the soft and porous fibers of young barley leaves are further digested and converted into highly palatable and easily edible fibers without the decomposition of lignin. It is an ideal dietary fiber that contains physiologically active ingredients and useful ingredients from barley grass green juice. This is an excellent dietary fiber that has a dietary effect on preventing hyperlipidemia, arteriosclerosis, diabetes, obesity, and even intestinal cancer, and is expected to have immune-enhancing and antitumor effects. be.

手続補正書 昭和５９年３　月１４日 特許庁艮１」　若　杉　４１」　夫　殿１、事件の表示 二「Ｊ樹内ｊ５９−２６８４−号 ２、発明の名称 憾のこＪ目のＡ久、１名カン六 ３補正をする者 事何との関係　特許出願人 住　所　−Ｆ＜、ＢＨ・早宝棒ｊ１］山本半井山荘４−
１４４代　理　人〒１０７ （Ｃよかｌネ、） （１）　明細書第１９＠第１行に、［核酸ミネラル］と
βるを、１ｒ核酸、ミイ・ラル」と訂正する。Procedural amendment March 14, 1980 Patent Office 1 Wakasugi 41 Husband 1 Case indication 2 J Kiuchi j59-2684- No. 2 Name of the invention ``Regret Noko J eye A Ku'' 1 person who makes the Kanroku3 amendment and their relationship Patent applicant address -F<, BH/Sawahobo j1] Yamamoto Hani Sanso 4-
144th Director 〒107 (C Yokalune,) (1) In the 19th line of the specification, [nucleic acid mineral] and βru should be corrected to 1r nucleic acid, mii lal.

（２）明細簀第２１頁の第２衣忙以下のとおり訂正する
。(2) The following corrections have been made to the second page on page 21 of the statement list.

Ｉｌ′　第２表 培養日数（Ｂ）　２０　３０ （日） 培養し６始からの成育期　３０　４３ 間総合計（日） ＊ 子実体収量　（ｒ）　８４．８　６６．１〔ビン当り〕 子実体の品質　浸み　良 ＊第３回目までの収量の合削帰　」Il′ Table 2 Culture days (B) 20 30 (Day) Growth period from the beginning of cultivation 30 43 Total time (days) * Fruiting body yield (r) 84.8 66.1 [per bottle] Quality of fruiting body: good soaking *Combined yields up to the 3rd round

Claims (1)

【特許請求の範囲】 １、：、きのこ類の人工栽培に艷し、イネ科植物の緑第
の青汁搾汁粕を培養基材としてなる培、養基に１ おいて人工栽培することを特徴とするきのこ類の栽樟方
法。 ２　該イネ科植物が稲類及、び麦類よりなる群からえら
ばれたイネ科植物である特許請求の範囲第１、項記載の
栽培方法。 ３、　きのこ類の人工栽培に、際し、イネ科植物の緑葉
の青汁搾汁粕を培養基材、とじてなる培養基において人
工栽培し、菌糸が該培奪基に蔓延した栽培生成物を自己
消化処理することを特徴とするきのこ類の栽培方法。 ４、　イネ科植物の成熟期前：５の緑葉の搾汁処理した
粕を有効成分とすることを特徴とするきのこ類の苗床人
工栽培用培養基材。[Scope of Claims] 1.: Artificial cultivation of mushrooms, using lees of pressed green juice of grasses as a culture substrate. A distinctive mushroom cultivation method. 2. The cultivation method according to claim 1, wherein the grass family plant is a grass family plant selected from the group consisting of rice and wheat. 3. For the artificial cultivation of mushrooms, the green juice lees of green leaves of a grass family plant are artificially cultivated in a culture medium consisting of a culture substrate, and the cultivation product in which mycelium spreads on the culture substrate is used. A method for cultivating mushrooms characterized by autolytic processing. 4. Before the maturity stage of a grass family plant: A culture substrate for artificial nursery cultivation of mushrooms, characterized in that the lees obtained by squeezing the green leaves of 5 is used as an active ingredient.