JPS6011136A - Method and device for dyeing microscope specimen - Google Patents
Method and device for dyeing microscope specimenInfo
- Publication number
- JPS6011136A JPS6011136A JP58120174A JP12017483A JPS6011136A JP S6011136 A JPS6011136 A JP S6011136A JP 58120174 A JP58120174 A JP 58120174A JP 12017483 A JP12017483 A JP 12017483A JP S6011136 A JPS6011136 A JP S6011136A
- Authority
- JP
- Japan
- Prior art keywords
- specimens
- processing liquid
- specimen
- holder
- slowly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 9
- 238000004043 dyeing Methods 0.000 title description 3
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 239000011521 glass Substances 0.000 claims abstract description 12
- 238000010186 staining Methods 0.000 claims description 13
- 230000001575 pathological effect Effects 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims 1
- 238000007664 blowing Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 description 5
- 239000007921 spray Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000000452 restraining effect Effects 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N2001/317—Apparatus therefor spraying liquids onto surfaces
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【発明の詳細な説明】
（技 術 分 野）
この発明は、病理検体の薄切片をスライドガラスに貼着
した細胞診用顕微鏡標本を染色する方法とこの方法の実
施に直接使用する装置とに関する。[Detailed Description of the Invention] (Technical Field) The present invention relates to a method for staining a microscopic specimen for cytology in which a thin section of a pathological specimen is pasted on a glass slide, and an apparatus used directly to carry out this method. .
（背 景 技 術）
癌その他の病気診断のため、患部等から採取した病理検
体の薄切片をスライドガラスに貼着して顕微鏡標本を作
り、これを顕微鏡で観察することか広く行なわれている
。このような顕微鏡で観察するだめの標本は、観察し易
くするために、細胞の組織を異なる部分毎に色を変えて
予め染色を施される。(Background technology) For diagnosis of cancer and other diseases, it is widely practiced to create a microscopic specimen by pasting a thin section of a pathological specimen taken from an affected area onto a glass slide, and then observing this specimen under a microscope. . In order to make these specimens easier to observe under a microscope, different parts of the cell tissue are stained in different colors in advance.
このため、従来から吊下げ式の籠の中に、スライドガラ
スに病理検体の薄切片を貼着した多数の標本を互いの間
に間隔をあけて多数収容し、この籠をハリスへマドキシ
リン、塩酸、エタノール等の処理液を入れた複数の処理
槽中に順次゛浸漬したり、或は上記のような標本を吊り
金具に支持させて複数の処理槽中に順次浸漬したりして
、標本の染色を行なっていた。For this reason, conventionally, a large number of specimens (thin sections of pathological specimens pasted to glass slides) are housed in a hanging cage, spaced apart from each other. , by sequentially immersing the specimen in multiple processing tanks containing a processing solution such as ethanol, or by supporting the specimen on a hanging bracket and sequentially immersing it in multiple processing tanks. I was doing some dyeing.
ところが、このようにして顕微鏡標本の染色を行なう場
合は、処理槽中の各種処理液がスライドガラスや籠の表
面に伺着したまま比較的多量に、次の処理槽にまで送ら
れ４前後の処理槽中の処理液が混合することか避けられ
ない。このようにして異種処理液の混合が生じると、処
理液の性能が劣化し、標本の染色が不鮮明になってしま
う。このため、従来も一定数の標本処理を行なったなら
ば、処理槽中の処理液を新しいものと交換するように作
業していたが、交換直前の処理液は性能が劣化している
ため、この液で処理された標本の染色は十分鮮明なもの
とはなり難かった。However, when staining a microscopic specimen in this way, a relatively large amount of the various processing solutions in the processing tank remain on the surface of the slide glass or cage and are sent to the next processing tank. Mixing of the processing liquids in the processing tank is unavoidable. When different types of processing liquids are mixed in this way, the performance of the processing liquid deteriorates and the staining of the specimen becomes unclear. For this reason, conventionally the processing solution in the processing tank was replaced with a new one after a certain number of specimens had been processed, but since the performance of the processing solution immediately before replacement had deteriorated, The staining of specimens treated with this solution was difficult to be sufficiently clear.
（本発明の目的）
本発明は上述のような不都合を解消し、常に鮮明な標本
染色を行なうことのできる方法とこの方法の実施に直接
使用す条装置とを提供することを目的としている。(Objectives of the Invention) The object of the present invention is to provide a method that eliminates the above-mentioned inconveniences and allows clear specimen staining at all times, and a strip device that can be used directly to carry out this method.
（本発明の構成）
ａ、標本を染色する方法の発明の構成
本発明の顕微鏡標本を染色する方法は、病理検体の薄切
片をスライドガラスに貼着した複数枚の顕微鏡標本を輪
状に配列して緩速回転させつつ、この標本に一つの処理
液を吹き付けた後、この処理液か作用する間そのまま緩
速回転を継続し、その後上記標本を高速回転させて各標
本の表面に伺着した処理液を遠心力により飛散させて排
除し、その後再び標本を緩く回転させつつ行なう別の処
理液の吹き付けおよび高速回転による余分の処理液の排
除を所定の処理液についてそれぞれ繰り返して顕微鏡標
本の染色を行なうものである。(Structures of the present invention) a. Structure of the invention for a method of staining a specimen The method of staining a microscopic specimen of the present invention involves arranging a plurality of microscopic specimens in which thin sections of pathological specimens are pasted on glass slides in a ring shape. One treatment liquid was sprayed onto the specimen while rotating slowly at the same time, and then the slow rotation was continued while the treatment liquid acted on it, and then the specimen was rotated at high speed to reach the surface of each specimen. The processing solution is scattered and removed by centrifugal force, and then another processing solution is sprayed while the specimen is gently rotated, and the excess processing solution is removed by high-speed rotation, which are repeated for each predetermined processing solution to stain the microscopic specimen. This is what we do.
ｂ、標本を染色する装置の発明の構成
本発明の顕微鏡標本を染色する装置は、病理検体の薄切
片をスライドガラスに貼着した複数枚の顕微鏡標本を輪
状に装着でき、中心部に挿入される回転軸に結合される
保持器と、この保持器を回転させるための速度変換自在
なモータと、上記保持器の外周を覆い、下端に排出口を
有するカバーと、このカバー内において保持器に装着さ
れた標本に処理液を吹き（くＪけるための噴霧器とから
構成される。b. Structure of the invention of apparatus for staining specimens The apparatus for staining microscopic specimens of the present invention is capable of mounting a plurality of microscope specimens in which thin sections of pathological specimens are affixed to glass slides in a ring shape, and is inserted into the center. a retainer connected to a rotating shaft; a motor capable of changing speed for rotating the retainer; a cover that covers the outer periphery of the retainer and has a discharge port at the lower end; It consists of a sprayer for spraying the processing solution onto the attached specimen.
（本発明の実施例）
次に、図示の実施例を説明しつつ本発明を更に訂しく説
明する。(Embodiments of the present invention) Next, the present invention will be explained in more detail while explaining the illustrated embodiments.
第１〜２図は、本発明により病理検体の薄切片をスライ
ドガラスに貼着した顕微鏡標本を染色する装置の全体構
成を示しており、第１図は縦断側面図、第２図は第１図
のＡ−Ａ断面図である。基台ｌの上面には、緩速と高速
とに速度変換自在なモータ２が固定Ｓれており、このモ
ータ２の、基台１の側方に大きく突出した回転軸３には
、病理検体の薄切片をスライドガラスに貼着した複数枚
（図示の例では１６枚）の標本４．４を放射状かつ円錐
輪状に装着する保持器５が、この回転軸３との相対的回
転不能に取付けられている。更に、この保持器５の周囲
には、この保持器５の外径よりも十分に大きな内径を有
する有底短円筒状のカバー６が設けられている。このカ
バー６のモータ２と反対側の端部は、回転軸３に保持器
５を着脱できるように開口しているが、後述するように
、カバー６内に噴霧されたり、標本から振り飛ばされる
処理液が外部に拡散しないように開閉自在な蓋を設ける
こともできる。カバー６の側面には、処理液噴霧用のノ
ズル７が設けられており、ホース８を送られて来る処理
液を圧縮空気によって、又は無気式にカバー６内に霧状
に吹込み、保持器５に装着された標本４．４のほぼ中央
部に貼着された検体の薄切片に吹き付けるようにしてい
る。Figures 1 and 2 show the overall configuration of an apparatus for staining a microscopic specimen in which a thin section of a pathological specimen is attached to a slide glass according to the present invention. It is an AA sectional view of the figure. A motor 2 that can freely change the speed between slow and high speeds is fixed on the top surface of the base l. A holder 5, which holds a plurality of specimens 4.4 (16 specimens in the illustrated example) in which thin sections of the sample 4.4 are pasted on slide glass in a radial and conical ring shape, is mounted so that it cannot rotate relative to the rotating shaft 3. It is being Furthermore, a short cylindrical cover 6 with a bottom and a sufficiently larger inner diameter than the outer diameter of the cage 5 is provided around the cage 5 . The end of the cover 6 opposite to the motor 2 is open so that the holder 5 can be attached to and detached from the rotating shaft 3, but as will be described later, spray may be sprayed into the cover 6 or shaken off from the specimen. It is also possible to provide a lid that can be opened and closed to prevent the processing liquid from diffusing to the outside. A nozzle 7 for spraying the processing liquid is provided on the side of the cover 6, and the processing liquid sent through the hose 8 is blown into the cover 6 in the form of a mist using compressed air or airlessly, and is held there. The spray is applied to a thin section of the specimen affixed to approximately the center of the specimen 4.4 mounted on the vessel 5.
更に、カバー６の下端には、標本４．４から振り飛ばさ
れた用済又は余分の処理液を排出するための排出口９が
設けられている。Furthermore, a discharge port 9 is provided at the lower end of the cover 6 for discharging the used or excess processing liquid that has been shaken off from the specimen 4.4.
一方、複数の標本４．４を装着するための保持器５は、
第３図に示すような主体１０と、第４図に示すような抑
え板１１と、両部材１０．１１を結合するためのナツト
片１２（第１図）とから成っている。第３図に示した主
体１０は、円板状の基板部１３の上面（上下は図面によ
る。）中央部に、前記回転軸３を挿通できる円管部１４
を垂直に植立し、この円管部１４の上部に上記基板部１
３と平行でこれよりもやや小径の円板状の支持板部１５
を設け、円管部１４の上端部の、支持板部１５よりも上
方に突出した部分の外周面には雄ねじ１６を形成してい
る。基板部１３の上面外周縁部には立壁１７が形成され
ており、この立壁１７の内側部分には、保持器５に装着
すべき標本の枚数と同数の放射状の溝１８．１８が環状
に配列されて形成されている。谷溝１８．１８は外周寄
り部分よりも内周寄り部分が深くなるように形成されて
いる。基板部１３と平行な支持板部１５には、標本の下
端を上記溝１８に嵌合させた際に、内周側に倒れようと
するこの標本４の上端部を支持するための複数の切込み
１９．１９が溝１８．１８に対応して溝１８．１８と同
方向に同数形成されている。On the other hand, the holder 5 for mounting a plurality of specimens 4.4 is
It consists of a main body 10 as shown in FIG. 3, a restraining plate 11 as shown in FIG. 4, and a nut piece 12 (FIG. 1) for connecting both members 10 and 11. The main body 10 shown in FIG. 3 includes a circular tube section 14 into which the rotating shaft 3 can be inserted, at the center of the upper surface (the upper and lower sides are according to the drawing) of a disk-shaped substrate section 13.
is planted vertically, and the substrate portion 1 is placed on top of this circular tube portion 14.
A disk-shaped support plate part 15 parallel to 3 and having a slightly smaller diameter than this.
A male thread 16 is formed on the outer circumferential surface of the upper end of the circular tube portion 14 in a portion that protrudes above the support plate portion 15 . A standing wall 17 is formed on the outer peripheral edge of the upper surface of the substrate section 13, and on the inner side of this standing wall 17, radial grooves 18 and 18 of the same number as the number of specimens to be mounted on the holder 5 are arranged in an annular shape. has been formed. The valley grooves 18.18 are formed so that the portions closer to the inner circumference are deeper than the portions closer to the outer circumference. A support plate section 15 parallel to the substrate section 13 has a plurality of notches for supporting the upper end of the specimen 4, which tends to fall toward the inner circumference when the lower end of the specimen is fitted into the groove 18. The same number of grooves 19.19 are formed in the same direction as the grooves 18.18, corresponding to the grooves 18.18.
抑え板１１は、上記主体１０の支持板部１５よりもやや
大径の円板で、中央には上記円管部１４の上端の雄ねじ
１６を形成した部分を挿通できる円孔２０が、下面周縁
部には垂下壁２１がそれぞれ形成されている。ナツト片
１２は、上記雄ねじ１６に螺合するものである。The holding plate 11 is a circular plate having a slightly larger diameter than the support plate part 15 of the main body 10, and has a circular hole 20 in the center thereof through which a male screw 16 at the upper end of the circular tube part 14 can be inserted, and a circular hole 20 on the lower surface periphery. A hanging wall 21 is formed in each portion. The nut piece 12 is screwed onto the male thread 16.
このように構成される保持器５に標本４．４を装着する
には、各標本４の下端縁を基板部上面の溝１８に嵌合さ
せ、上端部を支持板部の切込み１９に挿入する。溝１８
は内周寄り部分が外周寄り部分よりも深いため、溝１８
に下端縁を嵌合させた標本４の上端部は内周側に倒れよ
うとし、切込み１９にしっかりと保持される。このため
、主体１０を第３図の状態で机上等に置き、標本４．４
を装着する作業を、先に装着済の標本４．４の倒れ防止
を考慮することなく容易に行なうことができる。谷溝１
８．１８、切込み１９．１９に標本を装着したならば、
抑え板１１を支持板部１５に被せ、ナツト片１２を円管
部上端の雄ねじ１６に螺合させて支持板部１５を主体ｌ
Ｏに結合する。To attach the specimens 4.4 to the holder 5 constructed in this manner, the lower edge of each specimen 4 is fitted into the groove 18 on the upper surface of the base plate, and the upper end is inserted into the notch 19 in the support plate. . Groove 18
Since the inner circumference part is deeper than the outer circumference part, the groove 18
The upper end portion of the specimen 4 whose lower end edge is fitted into the specimen 4 tends to fall toward the inner circumferential side, and is firmly held in the notch 19. For this reason, the main body 10 is placed on a desk or the like in the state shown in FIG.
The work of mounting the specimen 4.4 can be easily carried out without having to consider preventing the mounted specimen 4.4 from falling over. Valley ditch 1
If the specimen is attached to 8.18 and notch 19.19,
Cover the support plate part 15 with the restraining plate 11, screw the nut piece 12 onto the male thread 16 at the upper end of the circular tube part, and attach the support plate part 15 to the main body l.
Bonds to O.
これにより、各標本４．４は外縁部上下端をそれぞれ立
壁１７と垂下［２１とによって支持されて、保持器５を
回転させても外れないよう番となる。As a result, each specimen 4.4 is supported by the vertical wall 17 and the hanging wall [21] at the upper and lower ends of its outer edge, so that it will not come off even when the holder 5 is rotated.
このようにして保持器５に複数の標本４を装着し、これ
に貼着された検体の薄切片を染色するには、保持器５を
横にしてその主体１０の中心の円管部１４内にモータ？
の回転軸３を挿通し、円管部１４の内部の通孔に形成し
た非円形断面部分と回転軸３に形成した非円形断面部分
とを嵌合させ、次いで円管部１４から突出した回転軸３
の先端に形成した雄ねじにす・ント片２２を螺合させ、
保持器５をこのナツト片２２と回転軸３の基部外周に形
成したフランジ２３との間で挟持する。次いで、モータ
２を緩速で回転す（Ｉｔ ツツ、／ ｉ Ｊｌ／ ７か
ら所定量の処理液をケース６内に噴Ｎｔ、る。噴霧され
た処理液は、標本４の表面番と付着する力く、この標本
４は回転軸３の周囲を公転しつつ自転するため、上記付
着した処理液は標本４の検体薄＋／Ｊ片およびスライド
ガラスの表面を往復するようしこ流れて、この表面をま
んべんなく濡らす。検体薄切片に付着した処理液が十分
作用するまでの間、モータ２は緩速で回転させる。次し
１でモータ２を急速回転させ、標本４．４の表面に付着
してｌ、Ｎる処理液を遠心力により振り飛ばす。振り飛
Ｉｆされた用済または余分の処理液は、カッ＼−６の内
面で捕捉されてカバー下端の排出口９から排出される。In order to attach a plurality of specimens 4 to the holder 5 in this way and stain the thin sections of the specimens affixed to the holder 5, the holder 5 is placed horizontally and placed inside the circular tube section 14 at the center of the main body 10. motor?
The rotating shaft 3 is inserted through the rotating shaft 3 , the non-circular cross-sectional part formed in the through hole inside the circular tube part 14 and the non-circular cross-sectional part formed in the rotating shaft 3 are fitted, and then the rotating shaft protruding from the circular tube part 14 is fitted. Axis 3
Screw the socket piece 22 onto the male thread formed at the tip of the
The retainer 5 is held between the nut piece 22 and a flange 23 formed on the outer periphery of the base of the rotating shaft 3. Next, the motor 2 is rotated at a slow speed (it/i Jl/ 7 to spray a predetermined amount of processing liquid into the case 6. The sprayed processing liquid adheres to the surface number of the specimen 4. Since the specimen 4 rotates on its own axis while revolving around the rotating shaft 3, the attached processing liquid flows back and forth over the specimen thin +/J piece of the specimen 4 and the surface of the slide glass. Wet the surface evenly. Rotate the motor 2 at a slow speed until the processing liquid attached to the specimen thin section is sufficiently affected. Next, rotate the motor 2 rapidly in step 1 to wet the surface of the specimen 4.4. Then, the processing liquid is shaken off by centrifugal force.The spent or excess processing liquid that has been shaken off is captured on the inner surface of the cup 6 and discharged from the discharge port 9 at the lower end of the cover.
それまで刺着していた処理液が総て振り飛１−２された
ならば、モータ２を再び緩速で回転させ、次の処理液を
噴霧して標本４．４に付着させる。When all of the processing liquid that had been stuck up to that point has been shaken off 1-2, the motor 2 is rotated at a slow speed again, and the next processing liquid is sprayed and attached to the specimen 4.4.
このようにして上述の行程を各処理液につｌ／Ｘて繰り
返して標本の染色を行なう。In this way, the above steps are repeated 1/X for each treatment solution to stain the specimen.
（木発１！１１の効果）
本発明の顕微鏡標本を染色する方法及び装置Ｃ土以」二
に述べたように構成されかつ実施されるため、異種の処
理液同士が混合して劣化を起すことがなく、常に新鮮な
処理液により鮮明な染色処理 ゛を行なうことができる
。(Effects of Kippatsu 1!11) Since the method and apparatus for staining microscopic specimens of the present invention are constructed and carried out as described in Section 2, different types of processing solutions mix with each other and cause deterioration. It is possible to always carry out clear dyeing processing using a fresh processing solution.
第１〜２図は本発明の装置の実施例を示しており、第１
図は縦断側面図、第２図Ｃ±第１図のＡ −Ａ断面図、
第３図は保持器の主体を示す途４視図、第４図は同じく
抑え板の斜視図である。
■＝基台、２：モータ、３：回転軸、４：標本、５：保
持器、６：カバー、７：ノズル、８：ホース、９：排出
口、１０：主体、ｌｌ：抑え板、１２：ナット片、１３
：基板部、１４：円管部、１５：支持板部、１６：雄ね
じ、１７：立壁、１８二溝、１９：切込み、２０：円孔
、２１：垂下壁、２２：ナツト片、２３：フランジ。
特許出願人 株式会社千代田製作所
代 理 人 小山欽造（ほか１名）
第１図
第２図
第３図
第４図1 and 2 show an embodiment of the device of the present invention, and the first
The figure is a vertical side view, Figure 2 C ± A-A sectional view of Figure 1,
FIG. 3 is a perspective view showing the main body of the cage, and FIG. 4 is a perspective view of the holding plate. ■=Base, 2: Motor, 3: Rotating shaft, 4: Specimen, 5: Holder, 6: Cover, 7: Nozzle, 8: Hose, 9: Discharge port, 10: Main body, ll: Holding plate, 12 : Nut piece, 13
: Substrate part, 14: Circular tube part, 15: Support plate part, 16: Male thread, 17: Vertical wall, 18 Two grooves, 19: Notch, 20: Circular hole, 21: Hanging wall, 22: Nut piece, 23: Flange . Patent applicant: Chiyoda Seisakusho Co., Ltd. Representative: Kinzo Koyama (and one other person) Figure 1 Figure 2 Figure 3 Figure 4
Claims (1)
枚の顕微鏡標本を輪状に配列して緩速回転させつつ、こ
の標本に一つの処理液を吹き付けた後、この処理液が作
用する間そのまま緩速回転を継続し、その後」二記標木
を高速回転させて各標本の表面に付着した処理液を遠心
力により飛散させて排除し、その後再び標本を緩く回転
させつつ行なう別の処理液の吹き付けおよび高速回転に
よる余分の処理液の排除を所定の処理液についてそれぞ
れ繰り返すことを特徴とする顕微鏡標本を染色する方法
。 ２）病理検体の薄切片をスライドガラスに貼着した複数
枚の顕微鏡標本を輪状に装着でき、中心部に挿入される
回転軸に結合される保持器と、この保持器を回転させる
ための速度変換自在なモータと、上記保持器の外周を覆
い、下端に排出口を有するカバーと、このカバー内にお
いて保持器に装着された標本に処理液を吹き付けるため
の噴霧器とから成る顕微鏡標本を染色する装置。[Scope of Claims] 1) A plurality of microscopic specimens in which thin sections of pathological specimens are pasted on glass slides are arranged in a ring shape and rotated slowly while a single processing solution is sprayed onto the specimens. Continue to rotate slowly while the treatment solution is applied, then rotate the marker at high speed to scatter and remove the treatment solution attached to the surface of each specimen using centrifugal force, and then slowly rotate the specimen again. 1. A method for staining a microscopic specimen, which comprises repeating spraying of another processing solution while applying a different processing solution and removing excess processing solution by high-speed rotation for each predetermined processing solution. 2) A holder connected to a rotating shaft that is inserted into the center and that allows multiple microscopic specimens with thin sections of pathological specimens pasted onto glass slides to be mounted in a ring, and a speed for rotating this holder. Staining a microscopic specimen, consisting of a convertible motor, a cover that covers the outer periphery of the holder and has a discharge port at the lower end, and a sprayer for spraying a processing liquid onto the specimen mounted on the holder within the cover. Device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58120174A JPS6011136A (en) | 1983-06-30 | 1983-06-30 | Method and device for dyeing microscope specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58120174A JPS6011136A (en) | 1983-06-30 | 1983-06-30 | Method and device for dyeing microscope specimen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6011136A true JPS6011136A (en) | 1985-01-21 |
| JPH0350972B2 JPH0350972B2 (en) | 1991-08-05 |
Family
ID=14779748
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58120174A Granted JPS6011136A (en) | 1983-06-30 | 1983-06-30 | Method and device for dyeing microscope specimen |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6011136A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0866748A1 (en) * | 1995-07-24 | 1998-09-30 | Innovation Instruments Inc. | Automated slide staining system |
| JP2002005798A (en) * | 2000-06-26 | 2002-01-09 | Japan Science & Technology Corp | Device for tissue dying |
| CN104568557A (en) * | 2014-12-22 | 2015-04-29 | 珠海迪尔生物工程有限公司 | Clamp for fixing slide |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3410093A4 (en) | 2016-01-29 | 2020-02-12 | Sysmex Corporation | Sample staining device, smear preparation apparatus and sample staining method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5343542A (en) * | 1976-10-01 | 1978-04-19 | Danieru Howaito Ronarudo | Method of and apparatus for automtically preparing microscope sample slides |
-
1983
- 1983-06-30 JP JP58120174A patent/JPS6011136A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5343542A (en) * | 1976-10-01 | 1978-04-19 | Danieru Howaito Ronarudo | Method of and apparatus for automtically preparing microscope sample slides |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0866748A1 (en) * | 1995-07-24 | 1998-09-30 | Innovation Instruments Inc. | Automated slide staining system |
| EP0866748A4 (en) * | 1995-07-24 | 2000-03-01 | Fisher Scient Company Llc | Automated slide staining system |
| JP2002005798A (en) * | 2000-06-26 | 2002-01-09 | Japan Science & Technology Corp | Device for tissue dying |
| CN104568557A (en) * | 2014-12-22 | 2015-04-29 | 珠海迪尔生物工程有限公司 | Clamp for fixing slide |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0350972B2 (en) | 1991-08-05 |
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