JPS5944396A - Method for cultivating mushroom - Google Patents
Method for cultivating mushroomInfo
- Publication number
- JPS5944396A JPS5944396A JP57155218A JP15521882A JPS5944396A JP S5944396 A JPS5944396 A JP S5944396A JP 57155218 A JP57155218 A JP 57155218A JP 15521882 A JP15521882 A JP 15521882A JP S5944396 A JPS5944396 A JP S5944396A
- Authority
- JP
- Japan
- Prior art keywords
- hot water
- flavanol
- extract
- spectrum
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Mushroom Cultivation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、針葉（でｄ類樹木内（（七Ｃの熱水抽出物、
該抽出物を有効成分とするトリコデルマ（・べ菌および
／まだｔよヒボクレア屈閑の防除剤、ならびに該防除剤
を用いる茸類栽培法に閂する。DETAILED DESCRIPTION OF THE INVENTION The present invention provides a hot water extract of coniferous (7C)
The extract is used as an effective ingredient for controlling Trichoderma and/or Hibocrea, as well as for mushroom cultivation methods using the same.
トリコデルマ属菌およびヒボクレアハ菌はｆ１ビζト１
栽培においてしばしば多大のＮ害を及ぼす害菌であるの
で、茸類栽培を日清に行っていくためにはこれらの害菌
の適しグな防除法が必要である。Trichoderma spp. and Hybocreaha spp.
Since they are harmful bacteria that often cause a great deal of N damage during cultivation, suitable control methods for these harmful bacteria are required in order to carry out mushroom cultivation on a daily basis.
従来、これらの菌の防除剤として合成化合物であるチア
ベンダゾール水和剤が用いられているが茸類の自然食品
としてのイメージダウンにつながるなどの理由から業者
の間ではこの化合物を防除剤として用いることを嫌うも
のが多い。Conventionally, thiabendazole hydrating powder, a synthetic compound, has been used as a control agent for these bacteria, but this compound is not used by manufacturers as a control agent for reasons such as reducing the image of mushrooms as a natural food. Many people dislike it.
従って、天然物の中で安全性の高い化合物を防除剤とし
て用いることができれば有利である。Therefore, it would be advantageous if a highly safe natural compound could be used as a pest control agent.
本発明者らは、天然に広く分布するフラバノール類およ
びそれを多Ｈに含有する金１葉イ・ｙ１類樹丙
木内（ｖｌ皮の熱水抽出物がトリコデルマＪ・チおよび
ハ
ヒボクレアに１（菌のバ９殖を抑え、茸類の増殖を促進
することを見出し本発明を完成するに至った。The present inventors have discovered that flavanols widely distributed in nature and a hot water extract of the skin of the genus A. y1, which contains flavanols in polyH. The present invention was completed based on the discovery that this method suppresses the growth of mushrooms and promotes the growth of mushrooms.
フラバノール類が種々の微生物に対し７て抗＾゛ｉ活性
を示すことは既に報告されている（ｓｏｉ］Ｓｃｉ、＋
胆、違２０３（１９６８）＋ ５ｏｉｉ ８Ｃｉ、＋
３μ。It has already been reported that flavanols exhibit anti-^゛i activity against various microorganisms (soi) Sci, +
Bile, difference 203 (1968) + 5oii 8Ci, +
3μ.
２３５（１９６９）＋ Ｍｉｃｒｏｂｉａ１ＥＣＯ１０
ｇ７＋ ４＋ ４２７（１９７８）’）が、トリコデ
ルマＰ’４ Ｗ４・ ヒボクレア属菌に対する抗１″Ｍ
活性に関しては知られてい々い。フラバノール類は、茶
あるいは果実々どに多Ｈに含有される化合物であり、食
品に関連する用揄に使用しても安全性がきわめて高い。235 (1969) + Microbia1ECO10
g7+ 4+ 427 (1978)') is an anti-1'M anti-Trichoderma P'4 W4 Hybocrea sp.
Not much is known about its activity. Flavanols are compounds found in high amounts in tea or fruits, and are extremely safe when used in food-related preparations.
以下本発明について詳細にＨ（シ明する。The present invention will be explained in detail below.
本発明は針葉柑類樹木内樹皮のｔ当木抽出物ならびに該
抽出物を有効成分とするトリコデルマ属菌および／また
はヒポ〃レア（・べ閘の防除剤を提供する。The present invention provides an extract of the inner bark of coniferous citrus trees and a control agent for Trichoderma and/or Hypolea, which contains the extract as an active ingredient.
本発明の熱水抽出物の１世法ならびに性〕ｔＩ■の一例
は以下のとおりである。An example of the method and property of the hot water extract of the present invention is as follows.
Ｉ、製法
スギ、ヒノキ、アカマツおよびカラマツから選ばれる＋
＆Ｊ木の生木より剥離した樹皮を約１０日間風乾したの
ち、外植・ｊ皮と内ｔｔｏｔ皮に分ける。内樹皮を切断
し７て小片としまたのち、ウィリー・ミルで粉砕し、１
６メツシ１１以上の粒度の粉体とする。この内樹皮ねを
１０〜２０倍量ノ蒸留水にｖ渕し、６５−１００’Ｃの
湯浴上で１〜５時間抽出を行なう。抽出後、抽出液を痙
取する。抽出液をロータリー・エバポレーターを用いて
減圧−１”、４（１〜５０℃で濃縮し、さらに凍結乾ヅ
！■により粉末状の抽出物とする。内樹皮乾正に対する
収木は４〜２０チである。I, manufacturing method selected from cedar, cypress, red pine and larch +
The bark peeled off from a live &J tree is air-dried for about 10 days and then divided into explant/j bark and inner ttot bark. The inner bark was cut into small pieces, and then ground in a Willy mill to give 1
Powder with a particle size of 6 to 11 or more. This inner bark layer is diluted with 10 to 20 times the amount of distilled water and extracted on a hot water bath at 65-100'C for 1 to 5 hours. After extraction, extract the extract. The extract is concentrated using a rotary evaporator under reduced pressure of -1", 4 (1 to 50°C), and then freeze-dried to a powdered extract. The yield for inner bark dryness is 4 to 20%. It is Chi.
ＴＩ 、 Ｋｉｔ木としてスギを用いて得られる熱水抽
出物の性質
（１）組成：有機成分：８７．２ｔＩ）〔このうちフラ
バノール類（バニリン−ｔＫ 酸比色
定Ｈ４゛法： Ｊ、 Ｓｃｉ 、 Ｆｏｏｄ Ａｇｒｉ
ｃ ｄη、 ７８８．１９７８） ４３．０係、水溶性
糖￥ｉ’ｉ （”ｃ−ＮＭＩＩスペクトル：第１図参照
） ４４．２係〕
無機成分：３２悴
水分：９．６％
（ＩＩ）有イエ（！成分元素分析（有機成分全体を１０
０とした場合の百分率）
ｃ ： ５１．９％、 Ｈ： ５．５％、 Ｏ： ４２
．５％、Ｎ：０．１％（ロ）フラバノール類の分子量お
よび平均分子柄〔木材学会誌、ヱ」・６７１（１９７９
） の方法に従って測定〕
分子晴分布： ３００〜３，０００（重合度：１〜１０
）平均分子シ″： ８３０（重合度：２，９）［フラバ
ノール単量体（（ト）−カブキン）の４１７９造式：
フラバノール重合体のイｆ）７ ；、：ｌ、式：ＦＴ
（ｉｖ） ”Ｃ−Ｎ ＭＲスペクト／ｌ／ （？（５１
図）（ｖ）工Ｒスペクトル（？ｌ’ ２図）（ｖｌ）色
：黄褐色
υ１０融点：混合物であるから明（ｊ（ｆな融点を示さ
ない。TI, Kit Properties of hot water extract obtained using cedar as a tree (1) Composition: Organic component: 87.2tI) [Among these, flavanols (vanillin-tK Acid colorimetric H4 method: J, Sci. Food Agriculture
c dη, 788.1978) 43.0, water-soluble sugar ￥i'i (c-NMII spectrum: see Figure 1) 44.2] Inorganic components: 32 Moisture: 9.6% (II) Yes (! Component elemental analysis (10% of all organic components)
Percentage when set to 0) c: 51.9%, H: 5.5%, O: 42
．． 5%, N: 0.1% (b) Molecular weight and average molecular pattern of flavanols [Journal of the Japan Society of Wood Science, E", 671 (1979
)] Molecular distribution: 300-3,000 (degree of polymerization: 1-10
) Average molecular size: 830 (degree of polymerization: 2,9) [4179 formula of flavanol monomer ((t)-kabuquin): flavanol polymer f)7 ;, :l, formula: FT (iv ) ”C-N MR spectrum/l/ (?(51
Figure) (v) Engineering R spectrum (?l' Figure 2) (vl) Color: yellowish brown υ10 Melting point: Bright because it is a mixture (j (f) does not show a melting point.
ＩＩＩ 、本発明の針葉樹類ｍ４木内トｙ、＋皮の熱水
抽出物は茸類栽培のときに発生し茸の生育障害となるト
リコデルマ属菌やヒポフレア属菌の生育を抑えることが
でき、かつ茸類の増殖促進の効果を有する。その効果ｉ
ｄ実施例で説明するが、効果の判定に用いる試験方法は
次のとおりである。III. The hot water extract of the conifer m4 kiuchi toy + skin of the present invention can suppress the growth of Trichoderma and Hypophleia bacteria that are generated during mushroom cultivation and cause problems with the growth of mushrooms, and It has the effect of promoting the growth of mushrooms. The effect i
dAs will be explained in Examples, the test method used to determine the effectiveness is as follows.
生育試験法
あらかじめ１２日間基本培地（グルコース５０ｒ、Ｊぐ
ｆ（２ＰＯ４１，５１ｉ’、 Ｋ、２ＨＰＯ４０，２ｒ
、ペプトン１．０ ？ ＋ ＭｆＳ０４・７Ｈ２００
，５ｆ、 Ｆｒ３Ｓ０４−７Ｈ，、ＤＯｏｏｌり」す
よび寒天３０２を１７！の蒸留水に溶解したもの）上で
前培養した供試菌〔シイタケｒ４ （Ｌｅｎｔｊ、ｎｕ
ｎ ｏｄｏｄｕｓ ＋７０１ ）＋ ） リコ
デルマＨハルジアナム（’Ｉ”ｒｊｃｈｏｄｅｒｍａｂ
ａｒＺｉＲｎｌｌｉｎ ） ！ ヒポフレア・コーグ
リソ１ンス（Ｈｙｐｏｃｒａａ ｎｉｇｒｉｃａ、ｎｓ
） ］を第３図に示すような生育測定用試験管内の培
地の一端に接種する。ナ妾１束後、ス４．６．８および
１６日層圧菌糸の生長最先端の位置をマジック・インキ
でチェックし、接種点からの長さを測って生長量とする
。生育試験用培地としては基本培地ならびにこれに清拭
５験物Ｊｐｉを添加したものを使う。Growth test method Basic medium (glucose 50r, Jgf (2PO41,51i', K, 2HPO40,2r
, Peptone 1.0? + MfS04・7H200
, 5f, Fr3S04-7H,, DOoool 17 agar 302! The test bacteria [Shiitake r4 (Lentj, nu
nododus +701)+) Lycoderma H harzianum ('I”rjchodermab
arZiRnllin)! Hypocraa nigrica, ns
)] is inoculated at one end of the culture medium in a test tube for growth measurement as shown in FIG. After 1 bundle, check the position of the leading edge of growth of stratified hyphae with a magic ink on the 8th and 16th day, and measure the length from the inoculation point to determine the amount of growth. As the culture medium for the growth test, a basic medium and a medium to which the 5-debrid test substance Jpi is added are used.
上記熱水抽出物は、トリコデルマＦべｒｔｑ 、 ヒ
ボクレア属ｈＭの防除剤として用いることができる。The above hot water extract can be used as a control agent for Trichoderma Fvertq and Hybocrea hM.
防除剤を培地にコ１．五用するときｔよ、防除剤を培地
に１〜３係添加すればよい。添加の時間はフｌへ常培地
作ＪｉｊＪ時とするが、栽培中に培地に添加しでもよい
。1. Add the pesticide to the culture medium. When using the insecticide, add 1 to 3 parts of the insecticide to the culture medium. The time of addition is at the time of regular culture cultivation, but it may be added to the culture medium during cultivation.
以下に本発明の実７１但例を示す。Seventy-one practical examples of the present invention are shown below.
実惰例１
スギの生木より剥晴した（０４皮を約１０日間風乾（含
水率約１０％）Ｌだのち、ナイフで外樹皮と内樹皮に分
別する。内ｉｒす皮をナイフで小片（約１ｏｘ３ｃｍ）
としたのち、さらにウイ、リー・ミルで粉砕し、１６メ
ツシユ以上の粒ＩＷの粉体とする。このようにして得ら
れだ自回皮粉を１０倍量の蒸留水に懸濁し、６５゛Ｃの
湯浴」二で３時間抽出を行なう。抽出後、抽出液と内１
（ｄ皮粉を戸別する。抽出液をロータリー・エバポレー
クを用いて減圧下、５０℃で〃Ｓ１縮し、さらに凍結μ
かＹとにより粉末状の抽出物とした。内樹皮乾重に対す
る収率１０７係。得られた抽出物の組成ならびに理化学
的性質は前記のとおり
実施例２
ヒノキ、アカマツおよびカラマツをスギに替える以外は
実施例１と同様に行ない、第１表に示す内樹皮熱水抽出
物を得た。Practical Example 1 Peeled bark from a live cedar tree (04) is air-dried for about 10 days (moisture content about 10%), and then separated into outer bark and inner bark with a knife. Cut the inner bark into small pieces with a knife. (approx. 1ox3cm)
After that, it is further ground in a Ui-Lee mill to obtain a powder with IW grains of 16 mesh or more. The autologous bark powder thus obtained was suspended in 10 times the amount of distilled water and extracted in a 65°C hot water bath for 3 hours. After extraction, extract liquid and 1
(d) The skin powder is sent from house to house.The extract is compressed at 50℃ under reduced pressure using a rotary evaporator, and then frozen
A powdered extract was prepared by using Y and Y. Yield 107 based on inner bark dry weight. The composition and physical and chemical properties of the obtained extract were as described above in Example 2. The procedure was carried out in the same manner as in Example 1 except that cypress, red pine, and larch were replaced with cedar, and the inner bark hot water extract shown in Table 1 was obtained. Ta.
ヒノキ （Ｃｈａｎ＋ｎｐｃｙｐａｒｉｓ ｏｂｔｕｅ
ａ） ４．７アソ７ マツ （Ｐｉｎ＋ｕ＋ ｃｌ
、ｅｎｓｉｆｌｏｒａ） １０．６カラ
マツ（Ｔ」ａｒｉｘ １ｅｐｔｏｌｅｐｉｓ）
６．４炭施例３
スギ内樹皮熱水抽出物添加培地における菌の生γ１：
上述の生育試験法において、生育試験用倍力ｌＬとして
基本培地にスギ内樹皮熱水抽出物を０．０゜１．０，２
．０および３．０％添加した培地を用いて、シイタケ、
トリコデルマ属１＾（、ヒポフレアｊ、’ｌ 庁（の生
育試験を行なった。なお対照として熱水抽出物に替えて
（ト）−カテキンを０〜１．０％用いる以外は同様の生
育試験を行なった。ｊ：、靭１．！コを第２表および第
３表に示す。Cypress (Chan+npcyparis obtue)
a) 4.7 aso 7 pine (Pin+u+cl
, ensiflora) 10.6 Larch (T'arix 1eptolepis)
6.4 Charcoal Example 3 Bacterial growth γ1 in a medium supplemented with cedar inner bark hot water extract: In the above-mentioned growth test method, 0.0.゜1.0,2
．． Using medium supplemented with 0 and 3.0%, shiitake,
A growth test was conducted on Trichoderma sp. The results are shown in Tables 2 and 3.
第２表 スギ内樹皮ｒ、へ水抽出物添加１７゛を地上で
の菌糸の相対生長［１１
第３表 （ト）−カテギン添加培地における菌の生育試
ｐ＠結果
対Ｊｉｍ（（第２表）の０）−カテキン添加培地では１
％の添加で、シイタケならびにトリコデルマ・ハルジア
ナムの生育仁よほぼ完全に抑えられ、ヒボクレア・ニグ
リカンスでは無添加の培地に比べその生ａは約３０係程
度まで抑えられた。Table 2 Relative growth of mycelia on the ground after addition of water extract to cedar inner bark r, 17゛ Table 3 ) of 0)-1 in catechin-added medium
% addition, the growth of Shiitake mushrooms and Trichoderma harzianum was almost completely suppressed, and the growth of Hybocrea nigricans was suppressed to about 30% compared to the medium without the addition.
これに対し、熱水抽出物添加（第２表）で（ｆよ、トリ
コデルマ・ハルジアナムおよびヒホクレア・ニグリカン
スに対し生育抑制効果を示すのに対し、シイタケに対し
てはかなりＷ！著な生育促進効果を示しだ。On the other hand, the addition of hot water extract (Table 2) showed a growth inhibiting effect on Trichoderma harzianum and Hyhocrea nigricans, but a significant growth promoting effect on Shiitake mushrooms. It shows.
′＃４ ）ｒｉ１２ 例４
実施例２で得た熱水抽出物を用い、実Ｋ［１例３と同（
子に行いトリコデルマ・ハルジアナム、ヒボクレア・ニ
グリカンスおよびシイタケの・情交」生長量を調べた。'#4) ri12 Example 4 Using the hot water extract obtained in Example 2, the same method as in Example 3 (
The growth of Trichoderma harzianum, Hibocrea nigricans, and Shiitake mushrooms was examined.
結果を第４表に示す。The results are shown in Table 4.
第４表 樹皮熱水抽出物添加培地上での菌糸の相対生長
−１１１
実施例５
供試菌としてヒラタケ、ナメコ、エノキタケ。Table 4: Relative growth of hyphae on a medium supplemented with bark hot water extract-111 Example 5 Oyster mushroom, Nameko mushroom, and Enoki mushroom as test bacteria.
ホンキクラゲおよびアラゲキクラゲを用い、スギの熱水
抽出物を２．０％添加で行なう以外は実施例３と同様に
行ない菌糸の相対生長ｆヶを調べた。結果を第５表に示
す。The relative growth of mycelia was examined in the same manner as in Example 3 except that 2.0% of a hot water extract of Japanese cedar was added using jellyfish and jellyfish. The results are shown in Table 5.
第５表 スギ内樹皮熱水抽出物添加培地上での食用４１
１子ｒ：”４顎のｌ’４ｊ糸の相対中長袖Table 5 Edible 41 on medium supplemented with cedar inner bark hot water extract
1st child r: 4-jaw l'4j yarn relative medium long sleeves
第１図はスギ内樹皮の、熱水抽出物（３０個ガ、５ｒａ
ｌ：の１３ｃｍｈｒＭｕスペクトルを示す。重化溶媒：
Ｄ、、Ｏ。
内部標準：メタノール（４９，８ｐｐｍ ）。
第２図はスギ内樹皮熱水抽出物のＩＲスペク） ＃ （
ＫＢｒ 錠剤法、試料／ ＫＢｒ ＝ ２／１５０Ｆｑ
）。
第３図は生育測定用試験管を示す。試！′ｌｔ管の親格
は、Ｐｙｒｅｘ岩城硝子（ｌｔＬ コードＡ ＴＥＳ
Ｔ１８ＮＰ、［コ形９寸法すム付、 １ｓｘｓｏ閲
を加］二し、培地流れ止め用のくびれを入れたもの。
図中１は培地流れ市め用のくびれ、２は接種点、３は紙
栓［Ｎｅｖｙ ５ｔｅｒｉ −Ｐｌｕｇ ／１６１６．
ノナコシ率品佳°０］、４は菌の生長方間、５け培用Ｌ
（約８ｍｌ ）を示す。
特ホ′「出願人 （１０２）協和醗酵工栗株式会社代表
者木下祝１’′１５“Figure 1 shows a hot water extract (30 pieces, 5ra) of the inner bark of Japanese cedar.
The 13 cmhrMu spectrum of 1: is shown. Heavy solvent:
D,,O. Internal standard: methanol (49.8 ppm). Figure 2 is the IR spectrum of the hot water extract of the inner bark of cedar)
KBr tablet method, sample/KBr = 2/150Fq
). Figure 3 shows a test tube for growth measurement. Try! 'lt tube's parent case is Pyrex Iwaki Glass (ltL code A TES
T18NP, [U-shaped with 9 dimensions, 1sxso view
2) and a constriction to prevent the medium from flowing. In the figure, 1 is a constriction for medium flow, 2 is an inoculation point, and 3 is a paper plug [Nevy 5teri-Plug /1616.
Nonakoshi rate: 0], 4 is for bacterial growth, L for 5-cell culture
(approximately 8 ml). ``Applicant (102) Kyowa Hakko Kokuri Co., Ltd. Representative Yuki Kinoshita 1''15''
Claims (1)
。 （＋） ｔｌｌ成：有（亮ｉ成分８７．２％（うちフ
ラバノールｊｊ、ｊ’１４３．０係、水溶性糖類４４，
２φ）・無イｊ）成分３２％、水分９．６係、 （（０有機成分元素分析値 （： ： ５１．９ ％、Ｈ：５．５ 佑 ０：４２
．５ ダｇ、 トｒ ： ０．１ チ（ｉｉｌ）
フラバノール層の分子材分布：３Ｑｌｌ〜３．０００
（重合度にして１〜１０） フラバノール類の平均分子量’ ： ８３００■）
Ｉ ３ｒコーＮ １．．４．１（スペクトル（出１１さ
：！、１ ）（ｖ）ＩＲスペクトル（第２図） （２）針葉樹９：１１１４木内■１皮の熱水拍出物を有
効成分トスるトリコデルマ属菌および／またはヒボクレ
ア属菌の防除剤。 ＋３＋ （’ｔ−４木がスギ、ヒノキ、７カマソおよ
びカラマツから週ばれることを％ｆ’文と、する４５’
、；１．？請求の範囲第２項記載の防除剤。 （４）特許請求の範囲？”、 ２１′ｒｉ配り・１巳の
防除剤をＪ［１いることを／１′￥徴とする二？％°チ
１１の栽培法。 （５）茸類がシイタケ、ホンキクラゲ、アラゲキクラゲ
、エノキタケ、ヒラフケ」７・よびナメコから選ばれる
ことを／ｌ￥ｆ：”にとする′：！￥Ｒ’Ｆ請求の範囲
第４項記載の栽培法。[Claims] (1) Having the above properties] - A hot water extract of the inner bark of a tree. (+) tll composition: Yes (light component 87.2% (including flavanol jj, j' 143.0%, water-soluble saccharide 44,
2φ)・No Ij) component 32%, moisture 9.6%, ((0 organic component elemental analysis value (: : 51.9%, H: 5.5 0:42
．． 5 Dag, Tr: 0.1 Chi (IIL)
Molecular material distribution of flavanol layer: 3Qll~3.000
(1 to 10 in terms of degree of polymerization) Average molecular weight of flavanols: 8300■)
I 3r Co N 1. ．． 4.1 (Spectrum (Output 11:!, 1) (v) IR spectrum (Figure 2) (2) Coniferous trees 9: 1114 Kiuchi ■ 1 Toss the hot water extract of the skin with the active ingredient Trichoderma spp. / or a control agent for fungi of the genus Hybocrea.
,;1. ? The pesticidal agent according to claim 2. (4) Scope of claims? ”, 21'ri distribution, 1 month of repellent, 2% °C 11 cultivation method where 1 month of repellent is treated as J [1 / 1' ¥ sign. The cultivation method according to claim 4, wherein /l\f:'' is selected from ``Hirafuke'' 7. and Nameko.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57155218A JPS5944396A (en) | 1982-09-08 | 1982-09-08 | Method for cultivating mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57155218A JPS5944396A (en) | 1982-09-08 | 1982-09-08 | Method for cultivating mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5944396A true JPS5944396A (en) | 1984-03-12 |
Family
ID=15601097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57155218A Pending JPS5944396A (en) | 1982-09-08 | 1982-09-08 | Method for cultivating mushroom |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5944396A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61212228A (en) * | 1985-03-12 | 1986-09-20 | キヤンベル ス−プ カンパニ− | Nutrient body for growing mushroom and its production |
| JPH02133556A (en) * | 1988-11-15 | 1990-05-22 | Furukawa Electric Co Ltd:The | Manufacture of extra fine sn-plated wire |
| EP0383430A2 (en) * | 1989-02-09 | 1990-08-22 | Monterey Mushrooms, Inc. | Control of fungal diseases in the production of mushrooms |
| EP0410319A2 (en) * | 1989-07-24 | 1991-01-30 | Takeda Garden Products Co., Ltd. | Spirogyra controlling and deodorant composition |
| JPH04293430A (en) * | 1991-03-19 | 1992-10-19 | Toyama Pref Gov | Culture base for mushroom |
| KR100393254B1 (en) * | 2001-03-21 | 2003-07-31 | 남경수 | A culture medium for Phellinus linteus and a culture method using thereof |
| CN104488544A (en) * | 2014-12-03 | 2015-04-08 | 李拴英 | Cultivation method of auriailaria polytricha |
| JP2021029219A (en) * | 2019-08-29 | 2021-03-01 | 株式会社ハイファ研究所 | Method for cultivating shiitake mushroom using coffee residue, and method for increasing functional components |
-
1982
- 1982-09-08 JP JP57155218A patent/JPS5944396A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61212228A (en) * | 1985-03-12 | 1986-09-20 | キヤンベル ス−プ カンパニ− | Nutrient body for growing mushroom and its production |
| JPH02133556A (en) * | 1988-11-15 | 1990-05-22 | Furukawa Electric Co Ltd:The | Manufacture of extra fine sn-plated wire |
| EP0383430A2 (en) * | 1989-02-09 | 1990-08-22 | Monterey Mushrooms, Inc. | Control of fungal diseases in the production of mushrooms |
| US5149715A (en) * | 1989-02-09 | 1992-09-22 | Monterey Mushroom, Inc. | Control of fungal diseases in the production of mushrooms |
| EP0410319A2 (en) * | 1989-07-24 | 1991-01-30 | Takeda Garden Products Co., Ltd. | Spirogyra controlling and deodorant composition |
| EP0410319A3 (en) * | 1989-07-24 | 1991-05-15 | Takeda Garden Products Co., Ltd. | Spirogyra controlling and deodorant composition |
| US5149534A (en) * | 1989-07-24 | 1992-09-22 | Takeda Garden Product Co., Ltd. | Spirogyra controlling and deodorant composition |
| US5298241A (en) * | 1989-07-24 | 1994-03-29 | Takeda Garden Product Co., Ltd. | Spirogyra controlling and deodorant composition |
| JPH04293430A (en) * | 1991-03-19 | 1992-10-19 | Toyama Pref Gov | Culture base for mushroom |
| KR100393254B1 (en) * | 2001-03-21 | 2003-07-31 | 남경수 | A culture medium for Phellinus linteus and a culture method using thereof |
| CN104488544A (en) * | 2014-12-03 | 2015-04-08 | 李拴英 | Cultivation method of auriailaria polytricha |
| JP2021029219A (en) * | 2019-08-29 | 2021-03-01 | 株式会社ハイファ研究所 | Method for cultivating shiitake mushroom using coffee residue, and method for increasing functional components |
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