JPS5883231A - Method and device for measuring characteristic of red blood cell - Google Patents
Method and device for measuring characteristic of red blood cellInfo
- Publication number
- JPS5883231A JPS5883231A JP56182563A JP18256381A JPS5883231A JP S5883231 A JPS5883231 A JP S5883231A JP 56182563 A JP56182563 A JP 56182563A JP 18256381 A JP18256381 A JP 18256381A JP S5883231 A JPS5883231 A JP S5883231A
- Authority
- JP
- Japan
- Prior art keywords
- red blood
- pores
- blood cells
- filter
- area
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003743 erythrocyte Anatomy 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims description 24
- 239000011148 porous material Substances 0.000 claims abstract description 55
- 210000004369 blood Anatomy 0.000 claims abstract description 12
- 239000008280 blood Substances 0.000 claims abstract description 12
- 239000002245 particle Substances 0.000 claims abstract description 11
- 238000001514 detection method Methods 0.000 claims description 24
- 239000003085 diluting agent Substances 0.000 claims description 11
- 229920003002 synthetic resin Polymers 0.000 abstract description 4
- 239000000057 synthetic resin Substances 0.000 abstract description 4
- 239000011521 glass Substances 0.000 abstract description 3
- 229910052594 sapphire Inorganic materials 0.000 abstract description 3
- 239000010980 sapphire Substances 0.000 abstract description 3
- 239000002184 metal Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 13
- 238000005259 measurement Methods 0.000 description 13
- 210000000601 blood cell Anatomy 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000000691 measurement method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000010979 ruby Substances 0.000 description 2
- 229910001750 ruby Inorganic materials 0.000 description 2
- 241000555745 Sciuridae Species 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000011545 laboratory measurement Methods 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
- 210000003429 pore cell Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、赤血球の特性、とくに赤血球が毛細管を通過
する際に様々な状態に変形する能力、すなわち変形能を
測定する方法およびその装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method and apparatus for measuring the properties of red blood cells, particularly the ability of red blood cells to deform into various states when passing through capillary tubes, that is, their deformability.
赤血球の種々の特性のうち、赤血球が血管内を絶えず循
環し、通常約８μ前後の直径を有するものが、直径数μ
足らずの毛細管内を自由に通過しているが、これは赤血
球が変形能を有しているためであり、この変形能を調べ
ることにより一微小循環障害などの診断に役立てること
ができる。一方、赤血球の変形能を定量化する手法の１
つとして、３μ程度のガラス毛細管に吸い込まれるに要
する除圧（吸引圧力）を一定のｐＨ，浸透圧で測定する
方法、または他の手法として、３〜５μ程度の多孔性の
フィルタに赤血球を透過させ流量を測定する方法が用い
られていたが、前者は顕微鏡下における赤血球１個宛の
測定であり、測定結果が必ずしも赤血球全体の平均値を
代表しているとは限らず、熟練を要し実用的ではない。Among the various characteristics of red blood cells, red blood cells constantly circulate within blood vessels and have a diameter of approximately 8 μm, whereas red blood cells have a diameter of several μm.
The red blood cells pass freely through the small capillaries, but this is because red blood cells have deformability, and examining this deformability can be useful in diagnosing microcirculatory disorders. On the other hand, one of the methods for quantifying the deformability of red blood cells is
One method is to measure the pressure relief (suction pressure) required for the red blood cells to be sucked into a glass capillary of about 3 μm using a constant pH and osmotic pressure, or another method is to pass red blood cells through a porous filter of about 3 to 5 μm. However, the former method involves measuring one red blood cell under a microscope, and the measurement result does not necessarily represent the average value for all red blood cells, and requires skill. Not practical.
後者は多数の赤血球に対する平均値が得られる反面、赤
血球の崩壊による誤差、赤血球を浮懸する液の粘度、温
度などの影響を受け、流量が必ずしも変形能に関連した
パラメータであるとは断定できない欠点があった。また
いずれの方法も測定に手間を要し、ｌ検体当りの測定所
要時間けかなシ長いものであった。上、記従来の測定手
法においては、血Ｒ１個に注目すると平均的な値が得ら
れず、一方、流量から変形能を測定する方法においては
、赤血球の１個１個についてはいかなる状態にあるかは
不明であるという間頂点があった。Although the latter allows obtaining an average value for a large number of red blood cells, it is affected by errors due to the collapse of red blood cells, the viscosity of the liquid in which the red blood cells are suspended, temperature, etc., and it cannot be concluded that the flow rate is necessarily a parameter related to deformability. There were drawbacks. Furthermore, both methods require time and effort for measurement, and the time required for measurement per 1 sample is long. In the above-mentioned conventional measurement method, when focusing on one blood R, an average value cannot be obtained.On the other hand, in the method of measuring deformability from the flow rate, it is difficult to obtain an average value for each red blood cell. There was a peak during which it is unknown.
本発明は上記の諸点に鑑みなされたもので、血液希釈液
を細孔に通過させ赤血球と希釈液との電気的差異に基づ
いて赤血球を検出する検出方法において、血液希釈液を
直径３μ前後の孔隙を多数有するフィルタに通過させた
後、前記細孔を通過させることにより、赤血球の変形能
を正確に測定することができる方法およびその装置を提
供せんとするものである。The present invention has been made in view of the above points, and is a detection method in which blood diluent is passed through a pore and red blood cells are detected based on the electrical difference between the red blood cells and the diluent. It is an object of the present invention to provide a method and an apparatus for accurately measuring the deformability of red blood cells by passing the red blood cells through a filter having a large number of pores and then passing through the pores.
本発明の第１の方法においては、液の流量を一定に保ち
、所定の時間内に検出される赤血球数により変形能を測
定するものである。したがって、従来の手法における単
位時間当シの流量から変形能を測定する方法に比べ、液
の流量、すなわち流速を一定に保つために、液の粘性や
温度による測定結果の影響を軽減できる。液の流量を一
定に保つ有効な手段として、ルビー、サファイアなどの
円板に直径１００μ前後の細孔を設け、細孔の裏側から
所定の圧力で吸引圧力を与える。圧力はたとえば２００
ｆｆＨｇ一定とすることによシ、細孔にりまシが生じな
い限り流速はほぼ一定に保たれる。In the first method of the present invention, the flow rate of the liquid is kept constant and the deformability is measured by the number of red blood cells detected within a predetermined period of time. Therefore, compared to the conventional method of measuring deformability from the flow rate per unit time, the influence of the viscosity and temperature of the liquid on the measurement results can be reduced because the flow rate of the liquid, that is, the flow velocity, is kept constant. As an effective means of keeping the flow rate of the liquid constant, pores with a diameter of about 100 μm are provided in a disk made of ruby, sapphire, etc., and suction pressure is applied from the back side of the pores at a predetermined pressure. For example, the pressure is 200
By keeping ffHg constant, the flow rate can be kept almost constant unless pores are clogged.
液の温度変化による液自体の粘度の影響を防止するため
に、恒温槽などによシ一定温度に保つことが望ましい。In order to prevent the viscosity of the liquid itself from being affected by changes in the temperature of the liquid, it is desirable to maintain the temperature at a constant temperature using a constant temperature bath or the like.
前記細孔は流速を一定に保つための流量調整用オリフィ
スだけでなく、血球と血球を浮懸する液とのインピーダ
ンスの差異に基づいて血球がオリフィスを通過する際に
血球を検出するための検出孔としての役目をも兼ねる。The pores are not only an orifice for adjusting the flow rate to maintain a constant flow rate, but also a detection orifice for detecting blood cells as they pass through the orifice based on the impedance difference between the blood cells and the liquid in which they are suspended. It also serves as a hole.
さらに前記細孔の前部（上流側）に、間隙の孔径が３μ
前後で少なくとも１万個以上の孔を有するフィルタを設
ける。すなわち、検出孔（細孔）の孔径が１００μ前後
で、間隙の孔径が３μ前後であるから、検出孔１個と間
隙１０００個前後とで両者の開孔の面積が等しくなり、
フィルタの孔隙の総面積が検出孔の面積の少なくともｌ
Ｏ倍以上有しないと、流速がフィルタで規制されること
になる。したがって、フィルタの孔面積を検出孔の面積
の１０倍程度以上−とすれば、流速は前記検出孔によっ
てのみ規制される。Furthermore, in the front part (upstream side) of the pore, the pore diameter of the gap is 3 μm.
A filter having at least 10,000 or more holes is provided at the front and rear. That is, since the pore diameter of the detection hole (pore) is around 100 μm and the pore diameter of the gap is around 3 μm, the area of 1 detection hole and around 1000 gaps are equal.
The total area of the pores of the filter is at least l of the area of the detection holes.
If it does not have O times or more, the flow rate will be regulated by the filter. Therefore, if the pore area of the filter is about 10 times or more the area of the detection hole, the flow rate is regulated only by the detection hole.
以上の手段によシ、５万倍程度に希釈された血液を前記
フィルタおよび検出孔が液面下にあるようにセットし、
検出孔の裏側から吸引圧力を与えると、赤血球が３μ程
度のフィルタを通過した後、検出孔で１個宛検出される
。所定の時間検出される血球数を計数することによシ、
フィルタを通過した血球の数が測定される。すなわち、
赤血球の直径は通常８μ程度であるが、フィルタの孔隙
は３μ前後であシ、このフィルタを通過するためには赤
血球の変形が必要である。したがって変形能を有する赤
血球のみが通過可能であり、たとえば溶血状態で通過し
たものは赤血球１個と数えられないために、変形能を数
値で表示することができる。測定時間はｌＯ秒〜１分程
度に設定されるが、あまりに長時間設定すると、変形能
を有しない赤血球、あるいけその他の粒子により目づま
°ｂを生じ、測定値に誤差を与える。Using the above method, blood diluted approximately 50,000 times is set so that the filter and detection hole are below the liquid level,
When suction pressure is applied from the back side of the detection hole, red blood cells are detected one by one at the detection hole after passing through a filter with a diameter of about 3μ. By counting the number of blood cells detected over a given period of time,
The number of blood cells passing through the filter is measured. That is,
The diameter of red blood cells is normally about 8μ, but the pores of the filter are around 3μ, and red blood cells must be deformed in order to pass through this filter. Therefore, only red blood cells that have deformability can pass through, and for example, red blood cells that pass in a hemolyzed state are not counted as one red blood cell, so the deformability can be expressed numerically. The measurement time is set to about 10 seconds to 1 minute, but if it is set too long, red blood cells, squirrels, and other particles that do not have deformability will cause clogging, giving an error in the measured value.
本発明の第２の方法は、フィルタの孔隙数を１０００個
かそれ以下、すなわちフィルタの孔隙の総画積が検出孔
の面積と等しいかそれ以下とする方法である。この方法
はフィルタ自体に検出孔に与えられる吸引圧力とほぼ同
じ圧力が与えられるために、温度による液の粘度変化の
影響を防止する必要がある。したがって、よシ高度の温
度管理下で測定する必要があふ。この方法においては、
所定量の試料（血液希釈液）を何秒かかつて吸引するこ
とが可能かを測定するものであり、検出孔は赤血球が正
常状態にあるか否かを監視するために用いることもでき
るし、フィルタ通過前の単位希釈液当りの粒子数を測定
することにより、フィルタ通過可能な粒子数とともに、
通過割合の重要なパラメータを得ることができ、所定の
液量の通過時間のパラメータとともに、赤血球の変形能
の特性を２次元のヒストグラムで表わすことができる。A second method of the present invention is a method in which the number of pores in the filter is 1000 or less, that is, the total area of the pores in the filter is equal to or less than the area of the detection hole. In this method, almost the same pressure as the suction pressure applied to the detection hole is applied to the filter itself, so it is necessary to prevent the influence of changes in liquid viscosity due to temperature. Therefore, it is necessary to perform measurements under very high temperature control. In this method,
It measures how many seconds it is possible to aspirate a predetermined amount of sample (blood dilution solution), and the detection hole can also be used to monitor whether red blood cells are in a normal state. By measuring the number of particles per unit diluted liquid before passing through the filter, we can calculate the number of particles that can pass through the filter, as well as the number of particles that can pass through the filter.
The important parameter of the passage rate can be obtained, and together with the parameter of the passage time of a given liquid volume, the characteristics of the deformability of the red blood cells can be expressed in a two-dimensional histogram.
つぎに以上の方法を実施するだめの本発明の装置の一実
施態様を図面に基づいて説明する。第１図は本発明の装
置の全体を示し、＠２ａは検出孔およびフィルタまわり
を示している。ｌＦｉ粒子検出装置で、血液希釈液２を
検出器３の下端に設けられた孔径１００μ前後の細孔４
（検出孔）に通過させ、赤血球と希釈液との電気的差異
に基づいて赤血球を検出するように構成されている。前
記細孔４を有する検出器３下端に、下部に直径３μ前後
の孔隙を多数有するフィルタ５を設けたアダプタ６を嵌
設している。７は細孔４を有するルビーまたはサファイ
アなどの硬質の材料からなる円板で、この円板７はガラ
スまたは合成樹脂からなる検出器３の先端（下端）に固
定され、細孔４を電路とし、検出器内外に検出電極８．
９が設けられている。１０け試料容器、１１け検出回路
、１２は流体制御装置、１３Ｆ！計数回路である。Next, one embodiment of the apparatus of the present invention for carrying out the above method will be described based on the drawings. FIG. 1 shows the entire device of the present invention, and @2a shows the detection hole and the area around the filter. In the lFi particle detection device, the blood diluent 2 is passed through a pore 4 with a pore diameter of approximately 100μ provided at the lower end of the detector 3.
(detection hole), and the red blood cells are detected based on the electrical difference between the red blood cells and the diluent. At the lower end of the detector 3 having the pores 4, an adapter 6 is fitted, which is provided with a filter 5 having a large number of pores with a diameter of about 3 μm at its lower part. Reference numeral 7 denotes a disk made of a hard material such as ruby or sapphire, which has pores 4. This disk 7 is fixed to the tip (lower end) of the detector 3 made of glass or synthetic resin, and uses the pores 4 as an electric path. , detection electrodes 8 inside and outside the detector.
9 is provided. 10 sample containers, 11 detection circuits, 12 fluid control device, 13F! It is a counting circuit.
フィルタ５は多孔性のメンブランフィルタなどを、必要
な孔隙数を除きパラフィン処理し、所定の個数分を有す
るようにしたもので、フィルタ固定用のアダプタ６に固
定される。アダプタ６は底部にフィルタ５を固定した円
筒容器状の形状を有し、内径は検出器３の先端−（下端
）部の外径と嵌合するようになっている。フィルタ５は
孔隙の総面積が細孔４の面積の１０倍以上となるか、ま
たは孔隙の総面積が細孔４の面積と等しいかそれ以下で
あるように設けられる。なおフィルタ５の孔隙の総面積
が細孔４の面積に比して十分に大きいときには、アダプ
タ６け軟質の合成樹脂の成形品でも十分であるが、フィ
ルタ５の孔隙の総面積が細孔４に等しいか小さいときに
は、アダプタ６は導電性金属で構成する必要がある。こ
れＦｉフィルタ５の孔隙の総面積が細孔４に比べて十分
に大きいときには、フィルタ５の孔隙を介し電路が確保
されるが、フィルタ５の孔隙の総面積が小さいときＶｃ
Ｉ／ｉ、フィルタ５の孔隙自体で粒子を検出してしまい
ノイズが発生するからである。また流体制御装置１２は
１ノメータ、ポンプなどを内蔵し、マノメータ接点によ
シボンプをオン、オフし、所定の吸引力を発生する。さ
らに検出回路１１にはタイマが内蔵され、本発明の第１
の方法においては、測定開始および測定終了信号を発し
、本発明の第２の方法においては、流体制御装置で定量
される液の時間測定を行なう。なおフィルタはアダプタ
ごと使い捨てとすることが望ましいが、蒸留水を逆流さ
せ、溶血、洗浄して再使用も可能である。それぞれの測
定に際しては、前回の赤血球の残渣を十分に拭き取如コ
ンタミネーションを防止する必要がある。The filter 5 is a porous membrane filter or the like that is treated with paraffin to remove the required number of pores so that it has a predetermined number of filters, and is fixed to an adapter 6 for fixing the filter. The adapter 6 has a cylindrical container shape with the filter 5 fixed to the bottom, and its inner diameter is adapted to fit with the outer diameter of the tip (lower end) of the detector 3. The filter 5 is provided so that the total area of the pores is 10 times or more the area of the pores 4, or the total area of the pores is equal to or less than the area of the pores 4. Note that when the total area of the pores in the filter 5 is sufficiently larger than the area of the pores 4, a molded product made of soft synthetic resin with 6 adapters is sufficient. , the adapter 6 must be made of conductive metal. When the total area of the pores of the Fi filter 5 is sufficiently larger than the pores 4, an electric path is secured through the pores of the filter 5, but when the total area of the pores of the filter 5 is small, Vc
This is because particles are detected in the pores of I/i and the filter 5 themselves, causing noise. Further, the fluid control device 12 has a built-in manometer, a pump, etc., and turns the pump on and off using the manometer contacts to generate a predetermined suction force. Furthermore, the detection circuit 11 has a built-in timer.
In the method described above, measurement start and measurement end signals are issued, and in the second method of the present invention, the time of the liquid to be quantified by the fluid control device is measured. Although it is desirable that the filter and the adapter be disposable, it is also possible to reuse it by backflowing distilled water, lysing the filter, and cleaning it. For each measurement, it is necessary to sufficiently wipe off the residual red blood cells from the previous measurement to prevent contamination.
本発明のいずれの方法においても、赤血球を１個宛検出
するために、検出パルスの高さが粒子の大きさに比例す
ることから、検出パルスの大きさを見ることによシ、赤
血球の状態をモニタすることができ、またいずれの方法
も数値が直接測定結果として得られ、かつ測定が容易で
、血液中の赤血球の平均の測定結果を得ることができる
。また従来、赤血球の変形能を直接示すパラメータがな
く、測定法によって種々のパラメータ、たとえば流電（
ｇ／／ｍ１ｎ）、フィルタ通過赤血球（％）または流量
Ｘ赤血球＆ （ｍｌ−ＲＢＣ／ ｍｉｎ ）などで表現
されており、一般に実験室的測定法であったが、汎用の
粒子計数装置にフィルタ機能を付加し、タイマを設ける
ことによシ、容易に変形能の測定を可能とし、また得ら
れる測定パラメータも数値表示として的確なものであり
、一般のルーチン用としても十分に価値が高いという効
果がある。In any of the methods of the present invention, in order to detect a single red blood cell, the height of the detection pulse is proportional to the particle size. In addition, both methods provide numerical values as direct measurement results, are easy to measure, and can provide average measurement results for red blood cells in the blood. Furthermore, until now, there has been no parameter that directly indicates the deformability of red blood cells, and depending on the measurement method, various parameters such as current (
g//m1n), red blood cells passing through a filter (%), or flow rate x red blood cells & (ml-RBC/min), etc., and was generally a laboratory measurement method, but a filter function was added to a general-purpose particle counter. By adding a timer and adding a timer, it is possible to easily measure the deformability, and the obtained measurement parameters are accurate as numerical displays, making it highly valuable for general routine use. There is.
第１図は本発明の装置の一実施態様を示す断面説明図、
第２図は検出器の細孔およびフィルタ部分の拡大断面図
であふ。
１・・・粒子検出装置、２・・・血液希釈液、３・・・
検出器、４・・・細孔、５・・・フィルタ、６・・・ア
ダプタ、７・・・円板、８．９・・・電極、１０・・・
試料容器、１１・・・検出回路、１２・・・流体制御装
置、１３・・・計数回路時 許 出 願 人 東亜医
用電子株式会社り
第２図FIG. 1 is a cross-sectional explanatory diagram showing one embodiment of the device of the present invention,
FIG. 2 is an enlarged cross-sectional view of the pore and filter portion of the detector. 1... Particle detection device, 2... Blood diluent, 3...
Detector, 4... Pore, 5... Filter, 6... Adapter, 7... Disc, 8.9... Electrode, 10...
Sample container, 11...Detection circuit, 12...Fluid control device, 13...Counting circuit Applicant: Toa Medical Electronics Co., Ltd. Figure 2
Claims (1)
気的差異に基づいて赤血球を検出する検出方法において
、血液希釈液を直径３μ前後の孔隙を多数有するフィル
タに通過させた後、前記細孔を通過させて、赤血球の変
形能を測定することを特徴とする赤血球の特性を測定す
る方法。 ２ フィルタの孔隙の総面積が細孔の面積の１０倍以上
であシ、所定時間に細孔を通過する赤血球数を検出する
特許請求の範囲第１項記載の赤血球の特性を測定する方
法。 ３ フィルタの孔隙の総面積が細孔の面積と等しいかそ
れ以下とし、所定体積の血液希釈液の通過時間を測定す
る特許請求の範囲第１項記載の赤血球の特性を測定する
方法。 ４ 血液希釈液を検出器下端に設けられた細孔に通過さ
せ赤血球と希釈液との電気的差異に基づいて赤血球を検
出する粒子検出装置において、細孔を有する検出器下端
に、下部に直径３μ前後の孔隙を多数有するフィルタを
設けたアダプタを嵌設してなることを特徴とする赤血球
の特性を測定する装置。 ５ フィルタの孔隙の総面積が細孔の面積の１０倍以上
である特許請求の範囲第４項記載の赤血球の特性を測定
する装置。 ６ フィルタの孔隙の総面積が細孔の面積と等しいかそ
れ以下である特許請求の範囲第４項記載の赤血球の特性
を測定する装置。[Claims] 1. In a detection method in which a blood diluent is passed through pores and red blood cells are detected based on the electrical difference between the red blood cells and the diluent, the blood diluent is passed through a filter having many pores with a diameter of about 3 μm. A method for measuring the characteristics of red blood cells, which comprises passing the red blood cells through the pores and then measuring the deformability of the red blood cells. 2. The method for measuring the characteristics of red blood cells according to claim 1, wherein the total area of the pores of the filter is 10 times or more the area of the pores, and the number of red blood cells passing through the pores in a predetermined time is detected. 3. The method for measuring the characteristics of red blood cells according to claim 1, wherein the total area of the pores of the filter is equal to or less than the area of the pores, and the passage time of a predetermined volume of blood diluent is measured. 4 In a particle detection device that detects red blood cells based on the electrical difference between the red blood cells and the diluent by passing a blood diluent through a pore provided at the lower end of the detector, a diameter A device for measuring the characteristics of red blood cells, characterized in that the adapter is fitted with a filter having a large number of pores of about 3 μm. 5. The device for measuring the characteristics of red blood cells according to claim 4, wherein the total area of the pores of the filter is 10 times or more the area of the pores. 6. The device for measuring the characteristics of red blood cells according to claim 4, wherein the total area of the pores of the filter is equal to or less than the area of the pores.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56182563A JPS5883231A (en) | 1981-11-13 | 1981-11-13 | Method and device for measuring characteristic of red blood cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56182563A JPS5883231A (en) | 1981-11-13 | 1981-11-13 | Method and device for measuring characteristic of red blood cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5883231A true JPS5883231A (en) | 1983-05-19 |
| JPH0247692B2 JPH0247692B2 (en) | 1990-10-22 |
Family
ID=16120456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56182563A Granted JPS5883231A (en) | 1981-11-13 | 1981-11-13 | Method and device for measuring characteristic of red blood cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5883231A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59961U (en) * | 1982-06-25 | 1984-01-06 | 東亜医用電子株式会社 | Device that measures the characteristics of blood cells |
-
1981
- 1981-11-13 JP JP56182563A patent/JPS5883231A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59961U (en) * | 1982-06-25 | 1984-01-06 | 東亜医用電子株式会社 | Device that measures the characteristics of blood cells |
| JPH0140049Y2 (en) * | 1982-06-25 | 1989-12-01 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0247692B2 (en) | 1990-10-22 |
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