JPS58209995A - Preparation of specimen for examination of body fluid component - Google Patents
Preparation of specimen for examination of body fluid componentInfo
- Publication number
- JPS58209995A JPS58209995A JP9066482A JP9066482A JPS58209995A JP S58209995 A JPS58209995 A JP S58209995A JP 9066482 A JP9066482 A JP 9066482A JP 9066482 A JP9066482 A JP 9066482A JP S58209995 A JPS58209995 A JP S58209995A
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- JP
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- Prior art keywords
- indicator
- components
- enzyme
- body fluid
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】 本発明は体液成分検査用試験片の製造法に関する。[Detailed description of the invention] The present invention relates to a method for manufacturing a test piece for testing body fluid components.
医療における臨床検査として、血液、リンパ液、尿等の
体液中に含有する病理学的成分の検査は、疾病の診断に
不可欠の情報を与えるものであってその意義は大きいも
のがある。かかる病理学的成分の検査方式としては一般
Ｃ：錠剤を用いる方式と試験片を用いる方式とがあり、
中でも後者はその使用上の操作が簡単で判定が短時間で
行なえ、又、近年の臨床検査Ｃ対する公衆の意識の高揚
により、一般家庭仁おいても自己の健康管理の一助とす
ることが出来る等の利点を有するので、その使用量が増
加しつつある。BACKGROUND ART As a clinical test in medical care, testing of pathological components contained in body fluids such as blood, lymph fluid, urine, etc. has great significance as it provides information essential for diagnosing diseases. There are two general testing methods for such pathological components: a method using tablets and a method using test pieces.
Among these, the latter is easy to use and can be used to make judgments in a short time, and with the recent heightened public awareness of clinical tests, it can also be used by ordinary households to help manage their own health. Because of these advantages, its usage is increasing.
従来、かかる用途Ｃ二側用する試験片としては、通常の
化学反応を利用していたが、近年、生理活性物ａである
酵素を用いる酵素的測定法カー、強酸、強アルカリ等を
使用せず、比較的緩や力１な条件で体液中の病理学的成
分を測定できるため（二多く利用されている。Conventionally, ordinary chemical reactions have been used as test pieces for such applications, but in recent years, enzymatic assay methods using enzymes, which are physiologically active substances a, strong acids, strong alkalis, etc. have been used. First, it is widely used because pathological components in body fluids can be measured under relatively gentle and forceful conditions.
例えば特公昭４５−１８７８号公報にはブドウ糖検出用
試験用品として吸収性材料６二、液体の酵素、過酸化反
応の働きを有する物質、緩衝系、オルト・トリジン・ジ
ヒドロ塩化物、ポリビニールピロリドン等の共重合体等
、および界面活性剤からなる液体混合物を含浸させてな
るものが記載されている。For example, Japanese Patent Publication No. 45-1878 describes test supplies for glucose detection such as absorbent materials 62, liquid enzymes, substances that act as peroxidants, buffer systems, ortho-tolidine dihydrochloride, polyvinyl pyrrolidone, etc. A product impregnated with a liquid mixture consisting of a copolymer, etc., and a surfactant is described.
しかしながら従来の試験用品は被検査液中の検査目的物
質の含有量に応じて色調変化するよ −う、酵素及び
指示薬等を水／アルコール系の水醇液を用いて濾紙（：
試薬の安定性を向上させるため多段階に含浸させ、その
後、含浸済の濾紙を適宜な大きさ、形状Ｃ二断裁し、更
Ｃ：シート状等の糸体に貼着して作製される。However, conventional test supplies use water/alcohol-based aqueous solution to store enzymes, indicators, etc. on filter paper (:
In order to improve the stability of the reagent, it is impregnated in multiple stages, and then the impregnated filter paper is cut into two pieces of appropriate size and shape C: C: It is produced by adhering to a thread body such as a sheet.
しかしながら、上記従来の作製方法における、水／アル
コール系の水溶液中における溶解した酵素は不安定で、
かつ、急速に変質するため、水溶液調整後直ちに多段階
含浸を行なう必要があり、製造上不便である上、品質を
均一じし、試験片の試験精度、信頼性を確保するため（
二は特別な注意と熟練を必要とする等、製造効率が低下
しやすく、従って製造コストも大きかった。However, the enzyme dissolved in the water/alcohol-based aqueous solution in the above conventional production method is unstable;
In addition, because the quality deteriorates rapidly, it is necessary to perform multi-step impregnation immediately after preparing the aqueous solution, which is inconvenient for manufacturing.
The second method requires special care and skill, which tends to reduce manufacturing efficiency, and therefore increases manufacturing costs.
本発明者は上記した従来の欠点に鑑みてなされたもので
あって、必要な酵素、指示薬等を他の成分と共−：非水
溶剤中に分散、混練することにより、水／アルコール系
の水溶液中じおける溶解した酵素等の不安定性、変質に
よる生理活性の消失等が解消されることを見出し、本発
明に到達したものである。The present inventor has devised a method in view of the above-mentioned conventional drawbacks, and by dispersing and kneading necessary enzymes, indicators, etc. in a non-aqueous solvent together with other components, a water/alcohol based solution has been developed. The present invention was achieved by discovering that the instability of dissolved enzymes and the like in an aqueous solution and the loss of physiological activity due to deterioration can be overcome.
即ち、本発明は、酵素と、指示薬と、結合剤及び添加剤
とを非水系溶剤中に分散し混練してなるインキ組成物を
用いて印刷法により基体に検査区域を設けることを特徴
とする、体液成分検査用試験片の製造法に関する。That is, the present invention is characterized in that an inspection area is provided on a substrate by a printing method using an ink composition prepared by dispersing and kneading an enzyme, an indicator, a binder, and an additive in a non-aqueous solvent. , relates to a method for manufacturing a test piece for testing body fluid components.
従来、酵素は有機溶剤中では不安定かつ変質されやすい
ものとされていたので非水溶媒中に凍結乾燥品の酵素を
分散、混練してなるインキ組成物を用いて安定かつ変質
しく二くい試験片が製造可能であることは予想し得なか
ったことＣある。非水溶媒中で安定かつ変質しＣ二（い
理由は明らかではない°、 ４、 、散
するのみであり、従って、分散状態では酵素を構成する
蛋白質の高次構造及び活性部位の変性を受けにくく、万
一、溶媒との界面近くで変性を受けても、内部にまでは
溶媒が浸透しにくいため大部分の酵素は活性を失なわな
いものと推定される。しかしながら非水溶媒と水の混合
系のよつに水を含んでいる溶媒を用いると活性が急速（
：失なわれるものである。Conventionally, enzymes were thought to be unstable and susceptible to deterioration in organic solvents, so tests were conducted to ensure stability and deterioration using an ink composition prepared by dispersing and kneading lyophilized enzymes in non-aqueous solvents. It was not foreseeable that the pieces could be manufactured. It is stable and denatured in non-aqueous solvents, and the reason for this is not clear. Therefore, in a dispersed state, the higher-order structure and active site of the protein constituting the enzyme are susceptible to denaturation. It is estimated that most enzymes do not lose their activity even if they are denatured near the interface with the solvent, as the solvent does not easily penetrate inside. When a solvent containing water is used in a mixed system, the activity increases rapidly (
: It is something that will be lost.
以下、本発明じついて順を追って詳細（：説明する。The present invention will be explained in detail below.
まず、本発明Ｃ：おいて用いる酵素（二ついて説明する
と、酵素としては、被検査液、特に体液中に含有する病
理学的成分、例えば、グルコース、コレステロール、尿
素、中性脂肪、リン脂質、アンモニア、乳酸、ピルビン
酸、シアル酸、Ｇ、ＦＴ％ＧＯ’ｌ’、コリンエステラ
ーゼ、ロイシンアミノベブチターゼ、アミラーゼ、ＬＯ
ＡＴ。First, the enzymes used in present invention C: (to explain in detail, the enzymes include pathological components contained in the test fluid, especially body fluids, such as glucose, cholesterol, urea, neutral fats, phospholipids, Ammonia, lactic acid, pyruvate, sialic acid, G, FT%GO'l', cholinesterase, leucine aminobebutitase, amylase, LO
A.T.
クレアチンホスホキナーゼ等と反応し、指示薬の色調変
化を起こすもの、若しくは、その反応によって、生じる
物質が更に別の酵素と反応して、指示薬の色調変化を、
起こすもの等を用いる。A substance that reacts with creatine phosphokinase etc. and causes a change in the color tone of the indicator, or a substance produced by the reaction further reacts with another enzyme, causing a change in the color tone of the indicator.
Use something that will wake you up.
次に指示薬としては、前記の酵素が病理学的成分と共に
作用し、その色調が変化するものを用いる。Next, as an indicator, one is used in which the above-mentioned enzyme acts together with a pathological component and its color tone changes.
酵素及び指示薬としては病理学的成分検出用として知ら
れている多くの組み合わせの中から適当に選択して用い
ることができる。The enzyme and indicator can be appropriately selected from among many combinations known for detecting pathological components.
例えばグルコースを検出するためには、酵素のが用いら
れる。この場合、グルコースオキシダーゼはグルコース
を酸化し、同時（二生じた過酸化水素がペルオキシダー
ゼの存在下でベンジジン系指示薬を変色させること（二
よりグルコースの検出が行なわれる。For example, to detect glucose, enzymes are used. In this case, glucose oxidase oxidizes glucose, and glucose is simultaneously detected by the hydrogen peroxide produced discoloring the benzidine indicator in the presence of peroxidase.
この（ｍ１尿酸、コレステロールを検出するためにはそ
れぞれ、ウリカーゼ、コレステロールオキシダーゼを作
用させて、生成する過酸化水素を同様にクロモゲンを配
した反応系で発色せしめて検出することができる。In order to detect this (m1 uric acid and cholesterol), uricase and cholesterol oxidase are allowed to act on each other, and the generated hydrogen peroxide can be similarly detected by coloring it in a reaction system equipped with chromogen.
以上の酵素及び指示薬を試験片の基体にパターン化して
固着させ、かつ、以上の酵素及び指示薬の被検査液中へ
の溶出を避けるため、結合剤を用いる。A binder is used to pattern and fix the enzyme and indicator to the substrate of the test piece, and to prevent the enzyme and indicator from eluting into the test liquid.
結合剤としては被検査液、特（二目的とする成分並びに
上記した酵素及び指示薬に影響を及ぼさず、又、試験を
妨げないものであることが必要であり、その都度適宜な
試験法によりチェックして使用することが望ましいが、
本発明者の実験によれば以下の各高分子化合物は使用可
能である。The binder must be one that does not affect the test liquid, special (dual-purpose components), enzymes and indicators mentioned above, and does not interfere with the test, and should be checked by appropriate test methods each time. However, it is preferable to use
According to the inventor's experiments, the following polymer compounds can be used.
合成高分子として、ポリアクリル酸エステル、ポリフェ
ニルアセタール、ポリメタクリレート、ポリアクリルア
ミド、ポリウレタン、ポリ塩化ビニル、ポリスチレン、
マレイン酸と他の成分とのポリマー類、ポリ酢酸ビニル
、ポリビニルアルコール、ポリビニルピロリドン等、半
合成高分子として、メチルセルロース、エチルセルロー
ス、プロピルセルロース等のセルロース誘導体、澱粉エ
ーテル等、
天然高分子として、澱粉、カゼイン、多糖類等。Synthetic polymers include polyacrylic ester, polyphenylacetal, polymethacrylate, polyacrylamide, polyurethane, polyvinyl chloride, polystyrene,
Polymers of maleic acid and other components, polyvinyl acetate, polyvinyl alcohol, polyvinylpyrrolidone, etc. Semi-synthetic polymers include cellulose derivatives such as methyl cellulose, ethyl cellulose, propyl cellulose, starch ether, etc. Natural polymers include starch, Casein, polysaccharides, etc.
以上の高分子化合物のうち、イソブチレン−無ホマレイ
ン酸コポリマー、ステレンーマレイ更に上記した酵素、
指示薬及び結合剤（二加えて、添加物として、試験片の
検査区域の吸水性及び保水性を向上させ、酵素及び指示
薬と被検量液中の検査目的成分である病理学的成分との
反応を円滑に進めるため、吸水性の担持体として、シリ
カ糸クレー、炭酸カルシウム、ケイ酸アルミニウム、ガ
ラス、セルロース等の微粒子を添加する。Among the above-mentioned polymer compounds, isobutylene-anfomaleic acid copolymer, sterene-maleic acid copolymer, and the above-mentioned enzymes,
Indicator and binder (2) In addition, as an additive, it improves the water absorption and water retention of the test area of the test piece, and prevents the reaction between the enzyme and indicator and the pathological component that is the test target component in the test volume solution. In order to proceed smoothly, fine particles such as silica thread clay, calcium carbonate, aluminum silicate, glass, and cellulose are added as water-absorbing carriers.
以上のような各成分を用いてインキ組成物を作成する方
法（二ついて述べると、まず凍結乾燥品の酵素、指示薬
及び必要Ｃ応じ緩衝剤等を粉砕し、およそ２５μ隅以下
の粒子とする。次（＝酵素、指示薬、緩衝剤の粒子を、
予め結合剤を排水溶媒（二醇解した結合剤溶液中に、吸
水性の担持体で−ある前記した微粉子と共に分散、混練
する。分散、混線（二は高速攪拌機、サンドミル、ボー
ルミル、ホモゲナイザー、３本ロール、超音波分散機等
が用いられる。A method for preparing an ink composition using each of the above-mentioned components (to describe two things: First, freeze-dried enzymes, indicators, and optionally C buffering agents, etc. are ground into particles with a corner size of about 25 μm or less. Next (=enzyme, indicator, buffer particles,
In advance, the binder is dispersed and kneaded in a drainage solvent (2) in a dissolved binder solution together with the above-mentioned fine powder which is a water-absorbing carrier. Three rolls, an ultrasonic disperser, etc. are used.
又、非水溶剤としてはアルコール類、ケトン類、芳香族
類、各種エステル類、炭化水素類等の有機溶剤のうちか
ら、結合剤の溶解性を考慮し、任意に選択して使用しう
るが有機溶剤はあらかじめ脱水して用いることが好まし
い。In addition, the non-aqueous solvent may be arbitrarily selected from organic solvents such as alcohols, ketones, aromatics, various esters, and hydrocarbons, taking into consideration the solubility of the binder. It is preferable to use the organic solvent after dehydrating it in advance.
なお、上記の有機溶媒中のメタノールを用いると酵素の
活性が、水を用いるときよりも緩やかではあるけれども
、やはり失なわれる傾向がある。Note that when methanol in the above-mentioned organic solvent is used, the activity of the enzyme still tends to be lost, albeit more slowly than when using water.
また、水ぬれ性、発色の均一性を向上させるため（二界
面活性剤等を添加することも出来、界面活性剤としては
、アニオン、カチオン、非イオン系のものが、いずれも
使用できる。Furthermore, in order to improve water wettability and uniformity of color development, a disurfactant or the like may be added, and any of anionic, cationic, and nonionic surfactants can be used.
以上のインキ組成物を用いて基体上に公知の印刷法によ
り検査区域を設ける。印刷法としては公知の印刷法がい
ずれも使用しうるが、本発明においては比較的塗布量が
多く、かつ、塗布量が一定な方式が好ましくその意味で
シルクスフジーン印刷、凹版印刷、グラビア印刷等が好
ましい。塗布量としては一概には言えないが通常２Ｐ／
が〜３０ｙ−／−（乾燥時）である。基体としては試薬
インキと反応しないもの、父、試薬の発色過程の反応を
阻害しないものであればいかなるものでもよく、例へは
紙、合成紙、各種プラスチックおよびフィルム、紙とプ
ラスチックをラミネートしたもの等が使用できる。An inspection area is provided on a substrate by a known printing method using the above ink composition. Any known printing method can be used as the printing method, but in the present invention, a method that allows a relatively large amount of coating and a constant amount of coating is preferable, and in that sense, silk printing, intaglio printing, gravure printing etc. are preferred. The amount of application cannot be generalized, but it is usually 2P/
is ~30y-/- (when dry). Any substrate may be used as long as it does not react with the reagent ink and does not inhibit the reaction of the reagent during the color development process. Examples include paper, synthetic paper, various plastics and films, and laminates of paper and plastic. etc. can be used.
以上のような本発明によれば、試験片上の検査区域中の
酵素、指示薬は安定であって変質しに＜＜、又、製造時
にもそれらを含むインキ組成物は安定で変質しじ（いた
め収り扱い上格別の注意を要せず、かつ、保存が可能で
あり、更に本発明においては印刷法６：よりパターン化
が可能であって工業的（二大量生産することは容易であ
る。According to the present invention as described above, the enzymes and indicators in the test area on the test piece are stable and do not deteriorate, and the ink composition containing them is stable and does not deteriorate during production. It does not require special care in storage and handling, and can be stored.Furthermore, in the present invention, printing method 6: Patterning is possible and industrial (2) easy to mass produce.
次（二実施例を挙げて、本発明の方法及び試験片の使用
上の効果を具体的に説明する。The following two examples are given to specifically explain the method of the present invention and the effects of using the test piece.
実施例 下記組成のグルコース検出用インキを調整した。Example A glucose detection ink having the following composition was prepared.
以上のインキはホモゲナイザーで微細分散させた後、ス
クリーン印刷法により、厚み３００μ噂の白色ポリスチ
レンフィルム上に一辺が６朋（２） ４角形となるよう
印刷した。用いたスフ９−フ版は１００ ｍｅｓｈ、レ
ジスト及びスクリーン紗の厚みの合計は１００μ調であ
った。得られた印刷物を８０°Ｃで１ｈｒ乾燥して試験
片を得た。The above ink was finely dispersed using a homogenizer, and then printed on a white polystyrene film with a thickness of 300 μm using a screen printing method to form a 6-square (2) square shape on each side. The S9-F plate used was 100 mesh, and the total thickness of the resist and screen gauze was 100 μm. The obtained printed matter was dried at 80°C for 1 hour to obtain a test piece.
得られた試験片を、既知のグルコース濃度の尿中に手早
く浸漬し、３０秒後に色調を測定した結果を次表（二示
す。次表における色調の表示はＪより Ｚ ８７２
１に定める標準色標によるものである。The obtained test piece was quickly immersed in urine with a known glucose concentration, and the color tone was measured after 30 seconds.The results are shown in the following table (2).The color tone in the following table is from J.Z 872
It is based on the standard color standard specified in 1.
次表からはｏ、 ｏ ｓ ｓのグルコース濃度が測定し
うろことがわかる。From the following table, it can be seen that the glucose concentration of o, o s s can be measured.
比較例１
上記組成の溶液を用いてろ紙（東洋ろ紙製、皇５１）Ｃ
含浸させ、５０°Ｃで２時間乾燥させ、試験紙を得た。Comparative Example 1 A filter paper (manufactured by Toyo Roshi Co., Ltd., Kou 51) C was prepared using a solution having the above composition.
It was impregnated and dried at 50°C for 2 hours to obtain a test paper.
比較例２
上記組成インキを実施例１と同様Ｃニスクリーン印刷法
によりフィルム上（二印刷パターン化１．４５°Ｃで２
時間乾燥して試験片を得た。Comparative Example 2 The above-mentioned ink composition was applied onto a film by the same C-screen printing method as in Example 1 (two-print patterning at 1.45°C).
A test piece was obtained by drying for a period of time.
実施例１、比較例１及び比較例２で得られた試験片を既
知の濃度のグルコース溶液中Ｃ二手早く浸漬した。それ
ぞれ予め設定した判定時間経過時の色調を表１に示す。The test pieces obtained in Example 1, Comparative Example 1, and Comparative Example 2 were briefly immersed in a glucose solution of a known concentration. Table 1 shows the color tones after each preset determination time elapsed.
判定に際して、グルコース濃度差が色調で最も判定しや
すい時間を選んだ。For the determination, we selected the time when the glucose concentration difference was most easily determined by color tone.
表Ｉ
実施例１のものが特にすぐれた定数性を示し、又、発色
後の色調の安定性が最つともすぐれていた。Table I Example 1 exhibited particularly excellent constant properties, and also had the best stability of color tone after color development.
次３二実施例、比較例１及び比較例２の試薬インキおよ
び含浸液の保存Ｃ二よる性能低下を調べた。The deterioration in performance of the reagent inks and impregnating liquids of Examples 32 and Comparative Examples 1 and 2 due to storage C2 was investigated.
試薬インキおよび含浸液を調製後４０°Ｃで１〜７日間
放置後試験片とし、０．５％グルコース溶液に含潰し、
各判定時の着色濃度を反射型濃度針で測定して性能の低
下を調べた。試薬の調製直後（＝試験片としたときの着
色濃度を１００とした相対濃度の測定結果を表１に示す
。After preparing the reagent ink and impregnating liquid, leave it at 40°C for 1 to 7 days, prepare a test piece, and impregnate it in a 0.5% glucose solution.
The coloring density at each judgment was measured using a reflective density needle to examine the deterioration in performance. Table 1 shows the measurement results of the relative concentration, with the coloring density taken as 100 immediately after the preparation of the reagent (= test piece).
表ａTable a
Claims (1)
分散し混練してなるインキ組成物を用いて印刷法Ｃ：よ
り基体に検査区域を設けることを特徴とする、体液成分
検査用試験片の製造７去。Printing method C using an ink composition prepared by dispersing and kneading an enzyme, an indicator, a binder, and an additive in a non-aqueous solvent: Body fluid component test characterized by providing a test area on a substrate 7. Manufacture of test pieces for use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9066482A JPS58209995A (en) | 1982-05-28 | 1982-05-28 | Preparation of specimen for examination of body fluid component |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9066482A JPS58209995A (en) | 1982-05-28 | 1982-05-28 | Preparation of specimen for examination of body fluid component |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2129652A Division JPH0687789B2 (en) | 1990-05-19 | 1990-05-19 | Composition for testing body fluid components |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58209995A true JPS58209995A (en) | 1983-12-07 |
| JPH0328199B2 JPH0328199B2 (en) | 1991-04-18 |
Family
ID=14004800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9066482A Granted JPS58209995A (en) | 1982-05-28 | 1982-05-28 | Preparation of specimen for examination of body fluid component |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58209995A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60178356A (en) * | 1984-02-24 | 1985-09-12 | Dainippon Printing Co Ltd | Body fluid tester |
| JPS60178358A (en) * | 1984-02-24 | 1985-09-12 | Dainippon Printing Co Ltd | Glucose detecting ink composition and tester formed by using the same |
| JPS61173797A (en) * | 1984-11-22 | 1986-08-05 | Ichikawa Kensou:Kk | Testpaper of mildewproofing agent |
| JPS61177997A (en) * | 1985-02-05 | 1986-08-09 | Konishiroku Photo Ind Co Ltd | Analytical element |
| JPS61247967A (en) * | 1985-04-26 | 1986-11-05 | Dainippon Printing Co Ltd | Inspecting material for detecting grape sugar and its production |
| JPH0315399A (en) * | 1990-05-19 | 1991-01-23 | Dainippon Printing Co Ltd | Composition for testing humor ingredient |
| US5183742A (en) * | 1984-02-24 | 1993-02-02 | Dai Nippon Insatsu Kabushiki Kaisha | Test device for detecting glucose, protein urobilinogen, and/or occult blood in body fluids and/or determining the PH thereof |
| US5728350A (en) * | 1992-08-21 | 1998-03-17 | Showa Yakuhin Kako Co., Ltd. | Chemical or microbiological test kit |
| US5955352A (en) * | 1994-12-22 | 1999-09-21 | Showa Yakuhin Kako Co., Ltd. | Instruments for chemical and microbiological tests |
| DE102011008899A1 (en) * | 2011-01-19 | 2012-07-19 | Usp Indicator Solutions Gmbh | Indicator for determining the skin condition |
-
1982
- 1982-05-28 JP JP9066482A patent/JPS58209995A/en active Granted
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60178356A (en) * | 1984-02-24 | 1985-09-12 | Dainippon Printing Co Ltd | Body fluid tester |
| JPS60178358A (en) * | 1984-02-24 | 1985-09-12 | Dainippon Printing Co Ltd | Glucose detecting ink composition and tester formed by using the same |
| US5183742A (en) * | 1984-02-24 | 1993-02-02 | Dai Nippon Insatsu Kabushiki Kaisha | Test device for detecting glucose, protein urobilinogen, and/or occult blood in body fluids and/or determining the PH thereof |
| JPS61173797A (en) * | 1984-11-22 | 1986-08-05 | Ichikawa Kensou:Kk | Testpaper of mildewproofing agent |
| JPS61177997A (en) * | 1985-02-05 | 1986-08-09 | Konishiroku Photo Ind Co Ltd | Analytical element |
| JPS61247967A (en) * | 1985-04-26 | 1986-11-05 | Dainippon Printing Co Ltd | Inspecting material for detecting grape sugar and its production |
| JPH0315399A (en) * | 1990-05-19 | 1991-01-23 | Dainippon Printing Co Ltd | Composition for testing humor ingredient |
| US5728350A (en) * | 1992-08-21 | 1998-03-17 | Showa Yakuhin Kako Co., Ltd. | Chemical or microbiological test kit |
| US5955352A (en) * | 1994-12-22 | 1999-09-21 | Showa Yakuhin Kako Co., Ltd. | Instruments for chemical and microbiological tests |
| DE102011008899A1 (en) * | 2011-01-19 | 2012-07-19 | Usp Indicator Solutions Gmbh | Indicator for determining the skin condition |
| DE102011008899A8 (en) * | 2011-01-19 | 2012-09-27 | Usp Indicator Solutions Gmbh | Indicator for determining the skin condition |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0328199B2 (en) | 1991-04-18 |
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