JPS5791197A - Reagent for measuring activity of substrate or enzyme - Google Patents
Reagent for measuring activity of substrate or enzymeInfo
- Publication number
- JPS5791197A JPS5791197A JP16428780A JP16428780A JPS5791197A JP S5791197 A JPS5791197 A JP S5791197A JP 16428780 A JP16428780 A JP 16428780A JP 16428780 A JP16428780 A JP 16428780A JP S5791197 A JPS5791197 A JP S5791197A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- enzyme
- acid
- reagent
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: A nonionic surfactant of a specific HLB value, an oxy derivative of cholanic acid or its salt and ethoxylated oleyl alcohol are added to the sample to remove the turbidity of the sample with no deactivation of enzymes, thus making it possible to measure the activity of the titled substances.
CONSTITUTION: (A) A nonionic surfactant of 10W13HLB value such as polyoxyethylene alkyl ether or polyoxyethylene alkyl phenyl ether and (B) at least one selected from the group consisting of oxy derivatives of cholanic acid such as cholic acid, deoxycholic acid, glycolic acid, their salts and ethoxylated oleyl alcohol are added to a buffer solution to prepare the reagent. An enzyme or coloring agent are added to the sample for measuring components in the sample together with the above reagent to measure the activity of the substrate or enzyme with no influence of the turbidity in the sample.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16428780A JPS5791197A (en) | 1980-11-20 | 1980-11-20 | Reagent for measuring activity of substrate or enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16428780A JPS5791197A (en) | 1980-11-20 | 1980-11-20 | Reagent for measuring activity of substrate or enzyme |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5791197A true JPS5791197A (en) | 1982-06-07 |
JPH0224520B2 JPH0224520B2 (en) | 1990-05-29 |
Family
ID=15790230
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP16428780A Granted JPS5791197A (en) | 1980-11-20 | 1980-11-20 | Reagent for measuring activity of substrate or enzyme |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5791197A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4845028A (en) * | 1982-11-19 | 1989-07-04 | Toyo Jozo Kabushiki Kaisha | Composition for lipase assay |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5089588A (en) * | 1973-12-07 | 1975-07-18 |
-
1980
- 1980-11-20 JP JP16428780A patent/JPS5791197A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5089588A (en) * | 1973-12-07 | 1975-07-18 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4845028A (en) * | 1982-11-19 | 1989-07-04 | Toyo Jozo Kabushiki Kaisha | Composition for lipase assay |
Also Published As
Publication number | Publication date |
---|---|
JPH0224520B2 (en) | 1990-05-29 |
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