JPS5772048A - Component analyzing method - Google Patents
Component analyzing methodInfo
- Publication number
- JPS5772048A JPS5772048A JP14813680A JP14813680A JPS5772048A JP S5772048 A JPS5772048 A JP S5772048A JP 14813680 A JP14813680 A JP 14813680A JP 14813680 A JP14813680 A JP 14813680A JP S5772048 A JPS5772048 A JP S5772048A
- Authority
- JP
- Japan
- Prior art keywords
- measurement
- final light
- reference value
- aref
- measuring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
PURPOSE:To perform a reliable and rapid measurement, by a method wherein, in a component analysis by a reaction speed measuring method for a biochemical component, a plurality of light measurements are conducted in a reaction process, and a selection is made through the comparison of a final light measurement value with a preset reference value. CONSTITUTION:In a measurement of an enzyme amount of a biochemical components, e.g., glutamic oxalacetic transminase (GOT) and the like, an absorbancy is measured at several points in a reaction process, and the results are compared with a reference value Aref previously within a range of a final light measuring time previously determined. In the case of a final light measurement value Aend, e.g., 5 in the Figure, exceeding the Aref, a reaction speed at a distance between measuring points 1-4 before a measuring point 5 is computed. In case the absorbancy Aend at the final light measuring point 5 is below the reference value Aref, a reaction speed at a distance between the mesuring points 1-5 is computed. The sufficent utilization of a reagent concentration in this manner enables to perform a highly precise measurement of the GOT so as to prevent the production of an eroneous result due to the deterioration or preparation error of the reagent.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14813680A JPS5772048A (en) | 1980-10-24 | 1980-10-24 | Component analyzing method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14813680A JPS5772048A (en) | 1980-10-24 | 1980-10-24 | Component analyzing method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5772048A true JPS5772048A (en) | 1982-05-06 |
JPH0121897B2 JPH0121897B2 (en) | 1989-04-24 |
Family
ID=15446067
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP14813680A Granted JPS5772048A (en) | 1980-10-24 | 1980-10-24 | Component analyzing method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5772048A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63271140A (en) * | 1987-04-28 | 1988-11-09 | Shimadzu Corp | Automatic rate analyzing method |
CN102680415A (en) * | 2011-03-16 | 2012-09-19 | 中国农业科学院作物科学研究所 | Colorimetric method for measuring the ketosuccinic acid content of the biological tissue |
-
1980
- 1980-10-24 JP JP14813680A patent/JPS5772048A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63271140A (en) * | 1987-04-28 | 1988-11-09 | Shimadzu Corp | Automatic rate analyzing method |
CN102680415A (en) * | 2011-03-16 | 2012-09-19 | 中国农业科学院作物科学研究所 | Colorimetric method for measuring the ketosuccinic acid content of the biological tissue |
Also Published As
Publication number | Publication date |
---|---|
JPH0121897B2 (en) | 1989-04-24 |
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