JPS5764160A - Method and apparatus for measuring urea - Google Patents
Method and apparatus for measuring ureaInfo
- Publication number
- JPS5764160A JPS5764160A JP55139606A JP13960680A JPS5764160A JP S5764160 A JPS5764160 A JP S5764160A JP 55139606 A JP55139606 A JP 55139606A JP 13960680 A JP13960680 A JP 13960680A JP S5764160 A JPS5764160 A JP S5764160A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- bro
- concentration
- measured
- contg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
Abstract
PURPOSE:To accrately measure urea concentration by acting urease-enzyme to the urea-contg. solution to be measured and allowing the resulting NH3 to contact and react with the solution contg. BrO<->, then detecting the change of BrO<-> concentration by polarography. CONSTITUTION:A prescribed amount of the solution to be measured is introduced from an inlet opening 1a of a sampling valve 1, and reacted with urease-enzyme 2 to produce NH3. After that, the reaction product is introduced to a flow cell 4 to transmit NH3 alone through a high polymer membrane 4c. On the other hand, an electrolytic solution contg. Br<-> is supplied from a container 8 for electrolytic solution to a reagent producing apparatus 7, and there BrO<-> is produced by electrolytic oxidation. Then it is introduced to the secondary chamber 4b to be reacted with said NH3. Consequently, the change of BrO<-> concentration is detected by polarography through electrodes 4d, 4e, 4f which are charged with onstant voltage from a potentiostat 5, and after prescribed signal treatment, the urea concentration in the solution to be measured in indicated in an indicater 6.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55139606A JPS5764160A (en) | 1980-10-06 | 1980-10-06 | Method and apparatus for measuring urea |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55139606A JPS5764160A (en) | 1980-10-06 | 1980-10-06 | Method and apparatus for measuring urea |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5764160A true JPS5764160A (en) | 1982-04-19 |
JPS6317181B2 JPS6317181B2 (en) | 1988-04-12 |
Family
ID=15249192
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55139606A Granted JPS5764160A (en) | 1980-10-06 | 1980-10-06 | Method and apparatus for measuring urea |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5764160A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2435510A (en) * | 2006-02-23 | 2007-08-29 | Mologic Ltd | Enzyme detection product and methods |
US7935497B2 (en) | 2006-02-23 | 2011-05-03 | Mologic Ltd | Protease detection |
US8241588B2 (en) | 2006-02-23 | 2012-08-14 | Mologic Ltd | Binding assay |
-
1980
- 1980-10-06 JP JP55139606A patent/JPS5764160A/en active Granted
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2435510A (en) * | 2006-02-23 | 2007-08-29 | Mologic Ltd | Enzyme detection product and methods |
US7935497B2 (en) | 2006-02-23 | 2011-05-03 | Mologic Ltd | Protease detection |
US8241588B2 (en) | 2006-02-23 | 2012-08-14 | Mologic Ltd | Binding assay |
US8361386B2 (en) | 2006-02-23 | 2013-01-29 | Mologic Ltd | Enzyme detection |
US8846328B2 (en) | 2006-02-23 | 2014-09-30 | Mologic Ltd | Method for detecting the presence of a protease enzyme in a sample |
Also Published As
Publication number | Publication date |
---|---|
JPS6317181B2 (en) | 1988-04-12 |
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