JPS5759167A - Production of latex reagent - Google Patents

Production of latex reagent

Info

Publication number
JPS5759167A
JPS5759167A JP13375180A JP13375180A JPS5759167A JP S5759167 A JPS5759167 A JP S5759167A JP 13375180 A JP13375180 A JP 13375180A JP 13375180 A JP13375180 A JP 13375180A JP S5759167 A JPS5759167 A JP S5759167A
Authority
JP
Japan
Prior art keywords
antigen
latex
reagent
antibody
contg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP13375180A
Other languages
Japanese (ja)
Other versions
JPS6116942B2 (en
Inventor
Toichi Yamada
Satoshi Obana
Michio Kuge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Priority to JP13375180A priority Critical patent/JPS5759167A/en
Publication of JPS5759167A publication Critical patent/JPS5759167A/en
Publication of JPS6116942B2 publication Critical patent/JPS6116942B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Polymerisation Methods In General (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

PURPOSE:To obtain a reagent exhibiting specific agglutination reaction of high sensitivity by sensitizing an antibody (antigen) with the latex obtd. by polymerizing styrene by the use of a water soluble radical initiator in the presence of a silicic acid anhydride of ultrafine particles then treating the same with an antibody (antigen)-contg. serum. CONSTITUTION:A silicic acid anhydride of ultrafine particles of about 5-50mmu grain sizes (''Aerosil '': Product of Nihon Aerosil Co., Ltd., or the like) is dispersed in water, and strene is polymerized by using a water soluble radical initiator such as K2S2O7 or the like in water, whereby latex of small variance in grain sizes of about 0.384mu average grain sizes is prepd. This latex is sensitized with an antibody (or antigen) at 7-8.6pH. A serum contg. an antibody (antigen) is further added to the sensitized latex and these are stirred for a short time, after which the serum treated latex is again suspended, whereby a latex reagent is prepd. When the resultant reagent is caused to react with a specimen contg. an antigen (an antibody when the reagent is sensitized with the antigen), it makes specific agglutination reaction, and the antigen is detected without the disturbance by the other protein or the like in the specimen and with high sensitivity. In this way, this reagent is adapted for detection of hepatitis B virus and the like.
JP13375180A 1980-09-25 1980-09-25 Production of latex reagent Granted JPS5759167A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13375180A JPS5759167A (en) 1980-09-25 1980-09-25 Production of latex reagent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13375180A JPS5759167A (en) 1980-09-25 1980-09-25 Production of latex reagent

Publications (2)

Publication Number Publication Date
JPS5759167A true JPS5759167A (en) 1982-04-09
JPS6116942B2 JPS6116942B2 (en) 1986-05-02

Family

ID=15112079

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13375180A Granted JPS5759167A (en) 1980-09-25 1980-09-25 Production of latex reagent

Country Status (1)

Country Link
JP (1) JPS5759167A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0659334U (en) * 1993-01-25 1994-08-19 明 生越 Magnet type iron carrier

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0381149U (en) * 1989-12-08 1991-08-20
JPH0429448U (en) * 1990-07-06 1992-03-10
WO1994015216A1 (en) * 1992-12-23 1994-07-07 Niyazmatov Agzamdzhan Akhtamov Process for obtaining a diagnostic reagent for detecting antigens and antibodies of infectious and other illnesses

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0659334U (en) * 1993-01-25 1994-08-19 明 生越 Magnet type iron carrier

Also Published As

Publication number Publication date
JPS6116942B2 (en) 1986-05-02

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