JPS5759167A - Production of latex reagent - Google Patents
Production of latex reagentInfo
- Publication number
- JPS5759167A JPS5759167A JP13375180A JP13375180A JPS5759167A JP S5759167 A JPS5759167 A JP S5759167A JP 13375180 A JP13375180 A JP 13375180A JP 13375180 A JP13375180 A JP 13375180A JP S5759167 A JPS5759167 A JP S5759167A
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- latex
- reagent
- antibody
- contg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Polymerisation Methods In General (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Abstract
PURPOSE:To obtain a reagent exhibiting specific agglutination reaction of high sensitivity by sensitizing an antibody (antigen) with the latex obtd. by polymerizing styrene by the use of a water soluble radical initiator in the presence of a silicic acid anhydride of ultrafine particles then treating the same with an antibody (antigen)-contg. serum. CONSTITUTION:A silicic acid anhydride of ultrafine particles of about 5-50mmu grain sizes (''Aerosil '': Product of Nihon Aerosil Co., Ltd., or the like) is dispersed in water, and strene is polymerized by using a water soluble radical initiator such as K2S2O7 or the like in water, whereby latex of small variance in grain sizes of about 0.384mu average grain sizes is prepd. This latex is sensitized with an antibody (or antigen) at 7-8.6pH. A serum contg. an antibody (antigen) is further added to the sensitized latex and these are stirred for a short time, after which the serum treated latex is again suspended, whereby a latex reagent is prepd. When the resultant reagent is caused to react with a specimen contg. an antigen (an antibody when the reagent is sensitized with the antigen), it makes specific agglutination reaction, and the antigen is detected without the disturbance by the other protein or the like in the specimen and with high sensitivity. In this way, this reagent is adapted for detection of hepatitis B virus and the like.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13375180A JPS5759167A (en) | 1980-09-25 | 1980-09-25 | Production of latex reagent |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13375180A JPS5759167A (en) | 1980-09-25 | 1980-09-25 | Production of latex reagent |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5759167A true JPS5759167A (en) | 1982-04-09 |
JPS6116942B2 JPS6116942B2 (en) | 1986-05-02 |
Family
ID=15112079
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP13375180A Granted JPS5759167A (en) | 1980-09-25 | 1980-09-25 | Production of latex reagent |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5759167A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0659334U (en) * | 1993-01-25 | 1994-08-19 | 明 生越 | Magnet type iron carrier |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0381149U (en) * | 1989-12-08 | 1991-08-20 | ||
JPH0429448U (en) * | 1990-07-06 | 1992-03-10 | ||
WO1994015216A1 (en) * | 1992-12-23 | 1994-07-07 | Niyazmatov Agzamdzhan Akhtamov | Process for obtaining a diagnostic reagent for detecting antigens and antibodies of infectious and other illnesses |
-
1980
- 1980-09-25 JP JP13375180A patent/JPS5759167A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0659334U (en) * | 1993-01-25 | 1994-08-19 | 明 生越 | Magnet type iron carrier |
Also Published As
Publication number | Publication date |
---|---|
JPS6116942B2 (en) | 1986-05-02 |
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