JPS5759157A - Enzyme electrode - Google Patents
Enzyme electrodeInfo
- Publication number
- JPS5759157A JPS5759157A JP55134907A JP13490780A JPS5759157A JP S5759157 A JPS5759157 A JP S5759157A JP 55134907 A JP55134907 A JP 55134907A JP 13490780 A JP13490780 A JP 13490780A JP S5759157 A JPS5759157 A JP S5759157A
- Authority
- JP
- Japan
- Prior art keywords
- coenzyme
- substrate
- immobilizing
- electrode
- adsorbing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To obtain an enzyme electrode responding quickly to a change in the concn. of substrate by adsorbing and immobilizing coenzyme on a conductive carrier, and immobilizing oxidoreductase to the resultant carrier by a cross-linking method. CONSTITUTION:A material which is conductive, has good adsorbing and holding power for coenzyme and is chemically stable, such as acetylene black, is molded under pressure as a binder, whereby an electrode substrate 1 is made. An aq. soln. of, for example, coenzyme nicotinamide adenine nucleotide (NAD) is developed on the substrate 1, after which it is dried, whereby an adsorbing and immobilizing layer 2 for coenzyme is provided. Next, an aq. soln. of oxidoreductase alcohol dehydrogenase is developed on the layer 2 and is dried, whereby an immobilizing layer 3 is formed. Thence these are caused to crosslink and react in the vapor of glutaraldehyde, whereby the enzyme electrode is obtained. This enzyme electrode is mounted in the bottom end part of a holder 7, and is immersed in buffer 8 contg. substrate (ethanol) 8, so that the analysis of good response speed is accomplished by using a device constituting a counter electrode 10, a salt bridge 9, a reference electrode 9, a potentiostat 5, and a recorder 4.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55134907A JPS5759157A (en) | 1980-09-27 | 1980-09-27 | Enzyme electrode |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55134907A JPS5759157A (en) | 1980-09-27 | 1980-09-27 | Enzyme electrode |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5759157A true JPS5759157A (en) | 1982-04-09 |
JPS6243496B2 JPS6243496B2 (en) | 1987-09-14 |
Family
ID=15139310
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55134907A Granted JPS5759157A (en) | 1980-09-27 | 1980-09-27 | Enzyme electrode |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5759157A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02209300A (en) * | 1989-02-10 | 1990-08-20 | Takara Co Ltd | Decorative body |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54140594A (en) * | 1978-04-24 | 1979-10-31 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
-
1980
- 1980-09-27 JP JP55134907A patent/JPS5759157A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54140594A (en) * | 1978-04-24 | 1979-10-31 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
Also Published As
Publication number | Publication date |
---|---|
JPS6243496B2 (en) | 1987-09-14 |
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