JPS5758893A - Analytical composition containing choline oxidase - Google Patents
Analytical composition containing choline oxidaseInfo
- Publication number
- JPS5758893A JPS5758893A JP11595481A JP11595481A JPS5758893A JP S5758893 A JPS5758893 A JP S5758893A JP 11595481 A JP11595481 A JP 11595481A JP 11595481 A JP11595481 A JP 11595481A JP S5758893 A JPS5758893 A JP S5758893A
- Authority
- JP
- Japan
- Prior art keywords
- choline oxidase
- choline
- derivative
- betaine
- determined
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: An analytical composition for choline, betaine or a derivative thereof containing a novel choline oxidase, 4-aminoantipyrine, peroxidase, etc. as a color reagent.
CONSTITUTION: A novel choline oxidase is collected from cultivated Arthrobacter globiformis B-0577 (FERM-P No.3518) by the conventional cultivating and collecting method. The resultant choline oxidase is then added to a solution of choline or a derivative thereof, e.g. lysolecithin, sphingomyelin or bytyrylcholine, or betaine aldehyde or a derivative thereof in an aqueous medium. The resultant hydrogen peroxide formed by the enzymic reaction is then determined by a peroxidase, etc. or the formed betaine is similarly determined by the Reinecke salt method. Alternatively, the reduced oxygen may be determined by an oxygen electrode.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11595481A JPS6053599B2 (en) | 1981-07-23 | 1981-07-23 | Analytical composition containing choline oxidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11595481A JPS6053599B2 (en) | 1981-07-23 | 1981-07-23 | Analytical composition containing choline oxidase |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51047992A Division JPS604716B2 (en) | 1976-04-26 | 1976-04-26 | Novel choline oxidase and its production method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5758893A true JPS5758893A (en) | 1982-04-08 |
JPS6053599B2 JPS6053599B2 (en) | 1985-11-26 |
Family
ID=14675253
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11595481A Expired JPS6053599B2 (en) | 1981-07-23 | 1981-07-23 | Analytical composition containing choline oxidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6053599B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6043334A (en) * | 1983-08-20 | 1985-03-07 | Maruichi:Kk | Preparation of raw material for dried small sardines and flakes of dried bonito |
-
1981
- 1981-07-23 JP JP11595481A patent/JPS6053599B2/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6043334A (en) * | 1983-08-20 | 1985-03-07 | Maruichi:Kk | Preparation of raw material for dried small sardines and flakes of dried bonito |
Also Published As
Publication number | Publication date |
---|---|
JPS6053599B2 (en) | 1985-11-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Borooah et al. | Studies on glucosaminidase. 2. Substrates for N-acetyl-β-glucosaminidase | |
Rattazzi et al. | Electrophoresis of glucose-6-phosphate dehydrogenase: a new technique | |
CA1185883A (en) | Method for the quantitative determination of bilirubin in biological fluids | |
AU588143B2 (en) | Process and reagent for the specific determination of the cholesterol of the hdl fraction | |
EP0167366A3 (en) | Accelerated nucleic acid reassociation method | |
Sato et al. | Thiobacillus ferrooxidans cytochrome c-552: purification and some of its molecular features | |
DE2716335A1 (en) | NEW CHOLINOXYDASE AND METHOD FOR PRODUCING IT | |
DE3173692D1 (en) | Lactase preparation | |
KR840004161A (en) | L-glutamic acid oxidase (H₂O₂ production), preparation method and analysis method | |
DE3311027A1 (en) | NAD (P) INDEPENDENT GLYCERINE DEHYDROGENASE, METHOD FOR THE PRODUCTION THEREOF AND THE USE THEREOF FOR DETERMINING GLYCERINE AND TRIGLYCERIDES | |
JPS5758893A (en) | Analytical composition containing choline oxidase | |
Hou et al. | Substrate specificity and stereospecificity of nicotinamide adenine dinucleotide-linked alcohol dehydrogenases from methanol-grown yeasts | |
JPS57110198A (en) | Determination of choline esterase activity | |
JPS57132879A (en) | Glutathione sulfhydryl oxidase and its preparation | |
JPS5543409A (en) | Estimation method of l-lysine | |
Silverman et al. | 2-(Fluoromethyl)-3-phytyl-1, 4-naphthoquinone and its 2, 3-epoxide. Inhibition of vitamin K epoxide reductase | |
Wood et al. | The electrophoresis and detection of transketolase | |
JPS5626197A (en) | Preparation of l-phenylalanine | |
JPS5764660A (en) | N-hydroxysulfoalkylaminine derivative and its application | |
JPS56142448A (en) | Engyme electrode | |
JPS5645198A (en) | Determination of amount of hydrogen peroxide of activity of peroxidase | |
JPS5758894A (en) | Analytical method with choline oxidase | |
JPS5779899A (en) | Kit for quantitative analysis of hydrogen peroxide | |
JPS5592697A (en) | Method for quantitative analysis using immobilized enzyme | |
JPS57146598A (en) | Determining method for reduced form of glutathine |