JPS5758629A - Separating and purifying method of colonization stimulating factor and callicrein in human urine - Google Patents

Separating and purifying method of colonization stimulating factor and callicrein in human urine

Info

Publication number
JPS5758629A
JPS5758629A JP55133262A JP13326280A JPS5758629A JP S5758629 A JPS5758629 A JP S5758629A JP 55133262 A JP55133262 A JP 55133262A JP 13326280 A JP13326280 A JP 13326280A JP S5758629 A JPS5758629 A JP S5758629A
Authority
JP
Japan
Prior art keywords
urine
aqueous solution
callicrein
substances
adjusted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55133262A
Other languages
Japanese (ja)
Other versions
JPS6159610B2 (en
Inventor
Fumimaro Takaku
Shinichi Morisue
Satoru Funakoshi
Morio Kuboyama
Nobuya Yanagiuchi
Muneo Yamada
Yoshiteru Watanabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Morinaga Milk Industry Co Ltd
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Morinaga Milk Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan, Morinaga Milk Industry Co Ltd filed Critical Green Cross Corp Japan
Priority to JP55133262A priority Critical patent/JPS5758629A/en
Publication of JPS5758629A publication Critical patent/JPS5758629A/en
Publication of JPS6159610B2 publication Critical patent/JPS6159610B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE: To separate the titled components useful as medicines, by bringing an aqueous solution containing a colonization stimulating factor (CSF) and callicrein derived from human urine into contact with a weakly basic anion exchanger, adsorbing both substances in the exchanger, and subjecting the substances to the salt concentration gradient elution method.
CONSTITUTION: The pH of a human urine as it is or an aqueous solution prepared by partially concentrating a colonization stimulating factor (CSF) derived from human urine and human uric callicrein is adjusted to 6.0W8.0, and the adjusted urine or aqueous solution is then concentrated to the uric proteinic concentration of preferably at least 40mg/mland heated to 55W65°C. The urine or aqueous solution is then adjusted to a pH of 0.4W5.0, mixed with polyethylene glycol and heated to precipitate and remove heat denatured substances. After removing the denatured substances, the pH is adjusted to 6.0W8.0 and the urine or aqueous solution is then brought into contact with a weakly basic anion exchanger to adsorb active constituents on the exchanger. The adsorbed substances are then subjected to the salt concentration gradient method at a pH of 6.0W8.0 to elute the uric CSF at a relatively low concentration and elute the callicrein at a relatively high concentration. Thus, the respective components are separated and purified.
COPYRIGHT: (C)1982,JPO&Japio
JP55133262A 1980-09-25 1980-09-25 Separating and purifying method of colonization stimulating factor and callicrein in human urine Granted JPS5758629A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55133262A JPS5758629A (en) 1980-09-25 1980-09-25 Separating and purifying method of colonization stimulating factor and callicrein in human urine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55133262A JPS5758629A (en) 1980-09-25 1980-09-25 Separating and purifying method of colonization stimulating factor and callicrein in human urine

Publications (2)

Publication Number Publication Date
JPS5758629A true JPS5758629A (en) 1982-04-08
JPS6159610B2 JPS6159610B2 (en) 1986-12-17

Family

ID=15100497

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55133262A Granted JPS5758629A (en) 1980-09-25 1980-09-25 Separating and purifying method of colonization stimulating factor and callicrein in human urine

Country Status (1)

Country Link
JP (1) JPS5758629A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2540121A1 (en) * 1983-01-28 1984-08-03 Green Cross Corp PROCESS FOR PREPARING COLONY STIMULATING FACTOR AND KALLIKREIN FROM HUMAN URINE
US5470569A (en) * 1985-02-05 1995-11-28 Cetus Oncology Corporation Recombinant colony stimulating factor-1
US5573930A (en) * 1985-02-05 1996-11-12 Cetus Oncology Corporation DNA encoding various forms of colony stimulating factor-1
EP0972566A3 (en) * 1998-07-13 2000-02-23 Minh Tran Quang Affinity immobilised metal resins
US6103224A (en) * 1985-02-05 2000-08-15 Chiron Corporation N∇2 CSF-1 (short form) and carboxy truncated fragments thereof
US6156300A (en) * 1985-02-05 2000-12-05 Chiron Corporation Point mutants of N∇2 CSF-1 and carboxy truncated fragments thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0121935Y2 (en) * 1985-05-16 1989-06-29

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2540121A1 (en) * 1983-01-28 1984-08-03 Green Cross Corp PROCESS FOR PREPARING COLONY STIMULATING FACTOR AND KALLIKREIN FROM HUMAN URINE
US5470569A (en) * 1985-02-05 1995-11-28 Cetus Oncology Corporation Recombinant colony stimulating factor-1
US5573930A (en) * 1985-02-05 1996-11-12 Cetus Oncology Corporation DNA encoding various forms of colony stimulating factor-1
US5643563A (en) * 1985-02-05 1997-07-01 Chiron Corporation N-∇3 deletion mutants of the short form of CSF-1
US5672343A (en) * 1985-02-05 1997-09-30 Chiron Corporation N- 3 deletion mutants of the long form of CSF-1
US5681719A (en) * 1985-02-05 1997-10-28 Chiron Corporation DNA encoding N- and C- terminally truncated colony stimulating factor-1 variants
US6103224A (en) * 1985-02-05 2000-08-15 Chiron Corporation N∇2 CSF-1 (short form) and carboxy truncated fragments thereof
US6117422A (en) * 1985-02-05 2000-09-12 Chiron Corporation N∇2-CSF-1(long form) and carboxy truncated fragments thereof
US6146851A (en) * 1985-02-05 2000-11-14 Chiron Corporation DNA encoding NV2 (long form) and carboxy truncated fragments thereof
US6156300A (en) * 1985-02-05 2000-12-05 Chiron Corporation Point mutants of N∇2 CSF-1 and carboxy truncated fragments thereof
US6204020B1 (en) 1985-02-05 2001-03-20 Chiron Corporation DNA encoding N∇2 CSF-1 (short form) and carboxy truncated fragment thereof
EP0972566A3 (en) * 1998-07-13 2000-02-23 Minh Tran Quang Affinity immobilised metal resins

Also Published As

Publication number Publication date
JPS6159610B2 (en) 1986-12-17

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