JPS57135361A - Latex reagent and measuring method of latex coagulation reaction - Google Patents

Latex reagent and measuring method of latex coagulation reaction

Info

Publication number
JPS57135361A
JPS57135361A JP2054781A JP2054781A JPS57135361A JP S57135361 A JPS57135361 A JP S57135361A JP 2054781 A JP2054781 A JP 2054781A JP 2054781 A JP2054781 A JP 2054781A JP S57135361 A JPS57135361 A JP S57135361A
Authority
JP
Japan
Prior art keywords
antibody
latex
reagent
styrene
hbs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2054781A
Other languages
Japanese (ja)
Other versions
JPS6352707B2 (en
Inventor
Toichi Yamada
Satoshi Obana
Michio Kuge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Priority to JP2054781A priority Critical patent/JPS57135361A/en
Publication of JPS57135361A publication Critical patent/JPS57135361A/en
Publication of JPS6352707B2 publication Critical patent/JPS6352707B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Polymerisation Methods In General (AREA)

Abstract

PURPOSE:To perform optical measurement for an antigen-antibody reaction with high sensitivity without occurring nonspecific coagulation by using a reagent of which an antibody is sensitized against a latex of which styrene and styrene sulfonic acid salts are polymerized without using an emulsifying agent under the existence of an ultrafine silicic anhydride. CONSTITUTION:Styrene and styrene sulfonic acid salts are copolymerized with a K2S2O8 used as an initiator under the existence of an ultrafine silicic anhydride having 5-50mmu particle size. Thereafter, said reactants are heated in pH value 8-11.5 at about 70 deg.C to obtain a latex having about 0.45mu average particle size. And, for example, an HBs antibody is combined with the latex. Then, said combined antibody is treated with a serum exclusive of an anti-human protein antibody from an antiserum of a guninea pig immunized against the HBs antibody to obtain a latex reagent. Further, after a patient serum contg. an HBs anti-antibody is reacted with the latex reagent, the absorbancy is measured. Thereby, said method can measure up to about 10<-8>g/sec antibody quantities and is highly sensitive as high as 1,000 times conventional cases.
JP2054781A 1981-02-13 1981-02-13 Latex reagent and measuring method of latex coagulation reaction Granted JPS57135361A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2054781A JPS57135361A (en) 1981-02-13 1981-02-13 Latex reagent and measuring method of latex coagulation reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2054781A JPS57135361A (en) 1981-02-13 1981-02-13 Latex reagent and measuring method of latex coagulation reaction

Publications (2)

Publication Number Publication Date
JPS57135361A true JPS57135361A (en) 1982-08-20
JPS6352707B2 JPS6352707B2 (en) 1988-10-19

Family

ID=12030171

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2054781A Granted JPS57135361A (en) 1981-02-13 1981-02-13 Latex reagent and measuring method of latex coagulation reaction

Country Status (1)

Country Link
JP (1) JPS57135361A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1416277A1 (en) * 2001-07-02 2004-05-06 Sekisui Chemical Co., Ltd. Carrier particle latex for assay reagent and assay reagent

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1416277A1 (en) * 2001-07-02 2004-05-06 Sekisui Chemical Co., Ltd. Carrier particle latex for assay reagent and assay reagent
EP1416277A4 (en) * 2001-07-02 2005-11-16 Sekisui Chemical Co Ltd Carrier particle latex for assay reagent and assay reagent
AU2002313319B2 (en) * 2001-07-02 2007-05-10 Sekisui Medical Co., Ltd. Carrier particle latex for assay reagent and assay reagent
EP2023141A3 (en) * 2001-07-02 2010-01-06 Sekisui Medical Co., Ltd. Carrier particle latex for assay reagent, and assay reagent

Also Published As

Publication number Publication date
JPS6352707B2 (en) 1988-10-19

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