JPS57120853A - Enzyme electrode - Google Patents

Enzyme electrode

Info

Publication number
JPS57120853A
JPS57120853A JP56006961A JP696181A JPS57120853A JP S57120853 A JPS57120853 A JP S57120853A JP 56006961 A JP56006961 A JP 56006961A JP 696181 A JP696181 A JP 696181A JP S57120853 A JPS57120853 A JP S57120853A
Authority
JP
Japan
Prior art keywords
electrode
immobilizing
pyruvic acid
enzyme
dried
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56006961A
Other languages
Japanese (ja)
Other versions
JPH0115018B2 (en
Inventor
Shiro Nankai
Akihiro Imai
Takashi Iijima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Priority to JP56006961A priority Critical patent/JPS57120853A/en
Publication of JPS57120853A publication Critical patent/JPS57120853A/en
Publication of JPH0115018B2 publication Critical patent/JPH0115018B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To improve the response property, by immobilizing pyruvic acid oxydase together with lactic acid dehydrogenase, nicotine amide adenine dinucleotide, on a collecting electrode. CONSTITUTION:An electrode base as a collector also serving as an immobilized carrier of an enzyme and coenzyme, is obtained by press-forming the mixture of carbon powder (graphite) and fluororesin powder as a binder. An aqueous solution of nicotine amide adenine dinucleotide is developed on this electrode base, and is dried. Successively, a mixed aqueous solution of lactic acid dehydrogenase and pyruvic acid oxydase is developed and dried. Then, glutaraldehyde functioning as an immobilizing agent is acted thereon and the resulting product is subjected to the immobilizing reaction, and is rinsed to yield an enzyme electrode. The electrode A thus obtained exhibits excellent sensibility compared with the electrode B not using pyruvic acid oxydase.
JP56006961A 1981-01-19 1981-01-19 Enzyme electrode Granted JPS57120853A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56006961A JPS57120853A (en) 1981-01-19 1981-01-19 Enzyme electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56006961A JPS57120853A (en) 1981-01-19 1981-01-19 Enzyme electrode

Publications (2)

Publication Number Publication Date
JPS57120853A true JPS57120853A (en) 1982-07-28
JPH0115018B2 JPH0115018B2 (en) 1989-03-15

Family

ID=11652806

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56006961A Granted JPS57120853A (en) 1981-01-19 1981-01-19 Enzyme electrode

Country Status (1)

Country Link
JP (1) JPS57120853A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6275251A (en) * 1985-09-25 1987-04-07 ビオセンソリ　ソチエタ　ペル　アツィオニ Bioelectric chemical battery and electrode for said battery
WO2001036955A1 (en) * 1999-11-16 2001-05-25 Matsushita Electric Industrial Co., Ltd. Biosensor
WO2002093151A1 (en) * 2001-05-15 2002-11-21 Matsushita Electric Industrial Co., Ltd. Biosensor

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6275251A (en) * 1985-09-25 1987-04-07 ビオセンソリ　ソチエタ　ペル　アツィオニ Bioelectric chemical battery and electrode for said battery
WO2001036955A1 (en) * 1999-11-16 2001-05-25 Matsushita Electric Industrial Co., Ltd. Biosensor
US6740215B1 (en) 1999-11-16 2004-05-25 Matsushita Electric Industrial Co., Ltd. Biosensor
WO2002093151A1 (en) * 2001-05-15 2002-11-21 Matsushita Electric Industrial Co., Ltd. Biosensor
US7235170B2 (en) 2001-05-15 2007-06-26 Matsushita Electric Industrial Co., Ltd. Biosensor

Also Published As

Publication number Publication date
JPH0115018B2 (en) 1989-03-15

Similar Documents

Publication Publication Date Title
US4376689A (en) Coenzyme immobilized electrode
EP0241309A3 (en) Measurement of electroactive species in solution
EP0125867A3 (en) Assay systems using enzymes requiring NAD(P) as cofactor
ES8701776A1 (en) Immobilized nucleic acid-containing probes.
NZ336841A (en) NADPH and NADH analogs such as 3-acetylpyridine adenine dinucleotide, 3-acetylpyridine-NADH, 3-acetylpyridine-NADPH, 3-pyridinealdehyde adenine dinucleotide for measurement of enzyme activities
JPS5381681A (en) Bio-reaction process
EP0173055A3 (en) Binding assay with amplified read-out and gas-phase detection
ES8106934A1 (en) A composition, test device and method of making it, and method for the determination of an analyte in a liquid.
JPS5510581A (en) Enzyem electrode
GB1527243A (en) Determination of isoenzymes
JPS5510583A (en) Enzyme electrode and its manufacture
DK159327C (en) PROCEDURE FOR BUTANOL PREPARATION FOR CARBON-CONTAINING COMPOUNDS WITH CLOSTRIDIUM ACETOBUTYLICUM ATCC 39236
JPS57120853A (en) Enzyme electrode
Ben-Bassat et al. Oxidation of C1-compounds in Pseudomonas C
JPS5796252A (en) Enzyme electrode
JPS55124060A (en) Enzyme electrode
JPS5512437A (en) Enzyme electrode
JPS5636048A (en) Enzyme electrode
EP0098145A3 (en) Process for producing gas diffusion electrode
GR3001508T3 (en) Process for the preparation of potassium nitrate
IE791149L (en) Preparation of 2-isopropylaminopyrimidine
ES478886A1 (en) Process for the isomerization of 3-vinyl-piperidine derivatives.
JPS5452033A (en) Preparation of selenoamino acid
GB1507375A (en) Process for preparing adenine
JPS5513072A (en) Electrochemical regeneration of coenzyme