JPS56653A - Ninhydrin reagent for new amino acid determination - Google Patents
Ninhydrin reagent for new amino acid determinationInfo
- Publication number
- JPS56653A JPS56653A JP7524979A JP7524979A JPS56653A JP S56653 A JPS56653 A JP S56653A JP 7524979 A JP7524979 A JP 7524979A JP 7524979 A JP7524979 A JP 7524979A JP S56653 A JPS56653 A JP S56653A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- added
- amino acid
- ninhydrin reagent
- buffer liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To quantify amino acid with a high precision, by using a ninhydrin reagent containing specific buffer liquid of a large buffer capability to reduce pH fluctuation of final coloring liquid and reduce fluctuation of the blank value and the coloring rate.
CONSTITUTION: Buffer liquid consisting of lithium acetate 3W5mol/l and free acetic acid 2W5mol/l is used. For example, rithium acetate 264g (4mol) is dissolved in water 600ml, and next, acetic acid 200ml (3.5mol) is added, and further, water is added to make the whole of the quantity of 1l, thus obtaining buffer liquid. Meanwhile, ninhydrin 20g and sodium borohydride 75mg are dissolved in mixture liquid of dimethylsulfoxide 450ml and methyl celloslve 300ml, and next, buffer liquid 250ml above is added to this solution, thus obtaining a ninhydrin reagent.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7524979A JPS56653A (en) | 1979-06-15 | 1979-06-15 | Ninhydrin reagent for new amino acid determination |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7524979A JPS56653A (en) | 1979-06-15 | 1979-06-15 | Ninhydrin reagent for new amino acid determination |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS56653A true JPS56653A (en) | 1981-01-07 |
Family
ID=13570756
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP7524979A Pending JPS56653A (en) | 1979-06-15 | 1979-06-15 | Ninhydrin reagent for new amino acid determination |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS56653A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0276930A (en) * | 1988-06-08 | 1990-03-16 | Loehr & Bromkamp Gmbh | Synchronous swivel joint with axial sliding characteristic |
JP2015536464A (en) * | 2012-11-21 | 2015-12-21 | ジェイピーピー クロマトグラフィー リミテッド | A novel ninhydrin reagent for the analysis of nitrogen-containing compounds |
-
1979
- 1979-06-15 JP JP7524979A patent/JPS56653A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0276930A (en) * | 1988-06-08 | 1990-03-16 | Loehr & Bromkamp Gmbh | Synchronous swivel joint with axial sliding characteristic |
JP2015536464A (en) * | 2012-11-21 | 2015-12-21 | ジェイピーピー クロマトグラフィー リミテッド | A novel ninhydrin reagent for the analysis of nitrogen-containing compounds |
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