JPS56653A - Ninhydrin reagent for new amino acid determination - Google Patents

Ninhydrin reagent for new amino acid determination

Info

Publication number
JPS56653A
JPS56653A JP7524979A JP7524979A JPS56653A JP S56653 A JPS56653 A JP S56653A JP 7524979 A JP7524979 A JP 7524979A JP 7524979 A JP7524979 A JP 7524979A JP S56653 A JPS56653 A JP S56653A
Authority
JP
Japan
Prior art keywords
liquid
added
amino acid
ninhydrin reagent
buffer liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP7524979A
Other languages
Japanese (ja)
Inventor
Kazuhiko Yamanishi
Masahiko Sugano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Priority to JP7524979A priority Critical patent/JPS56653A/en
Publication of JPS56653A publication Critical patent/JPS56653A/en
Pending legal-status Critical Current

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  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE: To quantify amino acid with a high precision, by using a ninhydrin reagent containing specific buffer liquid of a large buffer capability to reduce pH fluctuation of final coloring liquid and reduce fluctuation of the blank value and the coloring rate.
CONSTITUTION: Buffer liquid consisting of lithium acetate 3W5mol/l and free acetic acid 2W5mol/l is used. For example, rithium acetate 264g (4mol) is dissolved in water 600ml, and next, acetic acid 200ml (3.5mol) is added, and further, water is added to make the whole of the quantity of 1l, thus obtaining buffer liquid. Meanwhile, ninhydrin 20g and sodium borohydride 75mg are dissolved in mixture liquid of dimethylsulfoxide 450ml and methyl celloslve 300ml, and next, buffer liquid 250ml above is added to this solution, thus obtaining a ninhydrin reagent.
COPYRIGHT: (C)1981,JPO&Japio
JP7524979A 1979-06-15 1979-06-15 Ninhydrin reagent for new amino acid determination Pending JPS56653A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7524979A JPS56653A (en) 1979-06-15 1979-06-15 Ninhydrin reagent for new amino acid determination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7524979A JPS56653A (en) 1979-06-15 1979-06-15 Ninhydrin reagent for new amino acid determination

Publications (1)

Publication Number Publication Date
JPS56653A true JPS56653A (en) 1981-01-07

Family

ID=13570756

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7524979A Pending JPS56653A (en) 1979-06-15 1979-06-15 Ninhydrin reagent for new amino acid determination

Country Status (1)

Country Link
JP (1) JPS56653A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0276930A (en) * 1988-06-08 1990-03-16 Loehr & Bromkamp Gmbh Synchronous swivel joint with axial sliding characteristic
JP2015536464A (en) * 2012-11-21 2015-12-21 ジェイピーピー クロマトグラフィー リミテッド A novel ninhydrin reagent for the analysis of nitrogen-containing compounds

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0276930A (en) * 1988-06-08 1990-03-16 Loehr & Bromkamp Gmbh Synchronous swivel joint with axial sliding characteristic
JP2015536464A (en) * 2012-11-21 2015-12-21 ジェイピーピー クロマトグラフィー リミテッド A novel ninhydrin reagent for the analysis of nitrogen-containing compounds

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