JPS56162056A - Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles - Google Patents

Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Info

Publication number
JPS56162056A
JPS56162056A JP6543580A JP6543580A JPS56162056A JP S56162056 A JPS56162056 A JP S56162056A JP 6543580 A JP6543580 A JP 6543580A JP 6543580 A JP6543580 A JP 6543580A JP S56162056 A JPS56162056 A JP S56162056A
Authority
JP
Japan
Prior art keywords
latex particles
fragments
sensitizing
immunoglobulin
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6543580A
Other languages
Japanese (ja)
Other versions
JPS6341426B2 (en
Inventor
Noriyuki Tsubota
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eiken Chemical Co Ltd
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Priority to JP6543580A priority Critical patent/JPS56162056A/en
Publication of JPS56162056A publication Critical patent/JPS56162056A/en
Publication of JPS6341426B2 publication Critical patent/JPS6341426B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE:To determine the existing quantity of an antigen easily with good accuracy by sensitizing a mixture of immunoglobulin Facb fragments and pFc' fragments with latex particles and allowing the resultant sensitizing latex particles and the antigens in humors to react. CONSTITUTION:A mixture of immunoglobulin Facb fragments and pFc' fragments obtained by enzyme decomposition of immunoglobulin with plasmin is sensitized with latex particles of 0.1-1mum average particle sizes. Thence, the resultant sensitizing latex particles and humor sample are mixed, and the existing quantity of the anteigens is determined by such a method of deciding the degree of latex flocculation caused by the reaction of the antigens in the sample and the immunogloblin Facb fragments by naked eyes or irradiating light of 0.3-1.8mum wavelengths, and measuring the absorbancy thereof or the intensity of the scattered light. Thereby, the measurement is made without receiving the influence of RA factors or the like in spite of their existence in the samples.
JP6543580A 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles Granted JPS56162056A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6543580A JPS56162056A (en) 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6543580A JPS56162056A (en) 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Publications (2)

Publication Number Publication Date
JPS56162056A true JPS56162056A (en) 1981-12-12
JPS6341426B2 JPS6341426B2 (en) 1988-08-17

Family

ID=13287025

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6543580A Granted JPS56162056A (en) 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Country Status (1)

Country Link
JP (1) JPS56162056A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6175262A (en) * 1984-09-21 1986-04-17 Teijin Ltd Reagent for measuring immunological composite and measurement of composite using the same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6175262A (en) * 1984-09-21 1986-04-17 Teijin Ltd Reagent for measuring immunological composite and measurement of composite using the same
US4978611A (en) * 1984-09-21 1990-12-18 Teijin Limited Reagents for measurement of immune complexes and method for measurement of immune complexes by use thereof
JPH0322945B2 (en) * 1984-09-21 1991-03-27 Teijin Ltd

Also Published As

Publication number Publication date
JPS6341426B2 (en) 1988-08-17

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