JPS56147038A - Pretreatment for supravital stained specimen and automatic classifying apparatus using said pretreating method - Google Patents

Pretreatment for supravital stained specimen and automatic classifying apparatus using said pretreating method

Info

Publication number
JPS56147038A
JPS56147038A JP5043380A JP5043380A JPS56147038A JP S56147038 A JPS56147038 A JP S56147038A JP 5043380 A JP5043380 A JP 5043380A JP 5043380 A JP5043380 A JP 5043380A JP S56147038 A JPS56147038 A JP S56147038A
Authority
JP
Japan
Prior art keywords
blood cell
supravital
blood
org
microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP5043380A
Other languages
Japanese (ja)
Other versions
JPS6310787B2 (en
Inventor
Sumio Abe
Hajime Matsushita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP5043380A priority Critical patent/JPS56147038A/en
Publication of JPS56147038A publication Critical patent/JPS56147038A/en
Publication of JPS6310787B2 publication Critical patent/JPS6310787B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

PURPOSE:To automate pattern recognition of blood cell species by pretreating a supravital stained blood film specimen with a reagent consisting of an immersion oil and an org. solvent, them computer-processing the video signal and the like obtained from a microscope. CONSTITUTION:The supravital stained film specimen obtained by mixing and treating blood and staining liqiud is immersed for a prescribed time in an org. solvent mixed reagent, such as immersion oil-xylene of 1:9-1:19 ratios by using a pretreating device not illustrated, after which it is dried to allow the org. solvent to evaporate. The resultant specimen is set in a microscope 44. A TV camera 46 and a blood cell detector 58 are connected to the microscope 44, and the microspoce is automatically focused by the signal from the detector 58. Further, the blood cell detection signal of the detector 58 is inputted to a character extracting circuit 54 through computers 60, 56. The blood cell image signal from the camera 46 is inputted to an image monitor 50, a display device 52 and an image memory 48. The charactrs of the blood cells are extreacted and are made quantitative by the circuit 54 and the blood cell species are recognized with the computer 56.
JP5043380A 1980-04-18 1980-04-18 Pretreatment for supravital stained specimen and automatic classifying apparatus using said pretreating method Granted JPS56147038A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5043380A JPS56147038A (en) 1980-04-18 1980-04-18 Pretreatment for supravital stained specimen and automatic classifying apparatus using said pretreating method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5043380A JPS56147038A (en) 1980-04-18 1980-04-18 Pretreatment for supravital stained specimen and automatic classifying apparatus using said pretreating method

Publications (2)

Publication Number Publication Date
JPS56147038A true JPS56147038A (en) 1981-11-14
JPS6310787B2 JPS6310787B2 (en) 1988-03-09

Family

ID=12858728

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5043380A Granted JPS56147038A (en) 1980-04-18 1980-04-18 Pretreatment for supravital stained specimen and automatic classifying apparatus using said pretreating method

Country Status (1)

Country Link
JP (1) JPS56147038A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63208761A (en) * 1987-02-25 1988-08-30 Chiyoda Seisakusho:Kk Method and device for obtaining procedure to execute plural kinds of staining methods simultaneously by automatic staining device for microscopic specimen
JP2007514179A (en) * 2003-08-22 2007-05-31 ケーエルエー・テンコール・テクノロジーズ・コーポレーション Catadioptric imaging system for broadband microscope

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5298585A (en) * 1976-02-16 1977-08-18 Hitachi Ltd Cell sample preparation method
JPS5330383A (en) * 1976-09-01 1978-03-22 Taichi Ichihashi Method of sealing tissue pieces
JPS5372639A (en) * 1976-12-10 1978-06-28 Technicon Instr Method of fixing specimen and fixing device
JPS5377541A (en) * 1976-12-01 1978-07-10 Georges Michael P Living body clinics microscope specimen slide fixing device
JPS5381292A (en) * 1976-12-17 1978-07-18 Eastman Kodak Co Chemical analyzer
JPS53144761A (en) * 1977-05-23 1978-12-16 Saitorogisuka Sentoraruraborat Method and equipment of fixing piece of cover on slide holding microscope specimen
JPS5483887A (en) * 1977-12-16 1979-07-04 Iriyou Jiyouhou Shisutemu Kaih Tester for body to be tested

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5298585A (en) * 1976-02-16 1977-08-18 Hitachi Ltd Cell sample preparation method
JPS5330383A (en) * 1976-09-01 1978-03-22 Taichi Ichihashi Method of sealing tissue pieces
JPS5377541A (en) * 1976-12-01 1978-07-10 Georges Michael P Living body clinics microscope specimen slide fixing device
JPS5372639A (en) * 1976-12-10 1978-06-28 Technicon Instr Method of fixing specimen and fixing device
JPS5381292A (en) * 1976-12-17 1978-07-18 Eastman Kodak Co Chemical analyzer
JPS53144761A (en) * 1977-05-23 1978-12-16 Saitorogisuka Sentoraruraborat Method and equipment of fixing piece of cover on slide holding microscope specimen
JPS5483887A (en) * 1977-12-16 1979-07-04 Iriyou Jiyouhou Shisutemu Kaih Tester for body to be tested

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63208761A (en) * 1987-02-25 1988-08-30 Chiyoda Seisakusho:Kk Method and device for obtaining procedure to execute plural kinds of staining methods simultaneously by automatic staining device for microscopic specimen
US8675276B2 (en) 2003-02-21 2014-03-18 Kla-Tencor Corporation Catadioptric imaging system for broad band microscopy
JP2007514179A (en) * 2003-08-22 2007-05-31 ケーエルエー・テンコール・テクノロジーズ・コーポレーション Catadioptric imaging system for broadband microscope
JP2012212166A (en) * 2003-08-22 2012-11-01 Kla-Encor Corp Catadioptric imaging system for broad band microscopy

Also Published As

Publication number Publication date
JPS6310787B2 (en) 1988-03-09

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