JPS561351A - Simple fractionation and determination of serum deoxyribonucleic acid-binding protein closely related to canser by using gel cataphoresis and quantitative kit therefor - Google Patents
Simple fractionation and determination of serum deoxyribonucleic acid-binding protein closely related to canser by using gel cataphoresis and quantitative kit thereforInfo
- Publication number
- JPS561351A JPS561351A JP7661579A JP7661579A JPS561351A JP S561351 A JPS561351 A JP S561351A JP 7661579 A JP7661579 A JP 7661579A JP 7661579 A JP7661579 A JP 7661579A JP S561351 A JPS561351 A JP S561351A
- Authority
- JP
- Japan
- Prior art keywords
- gel
- binding protein
- cataphoresis
- serum
- deoxyribonucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To provide a method of determination of deoxyribonucleic acid-binding protein useful for a clinical diagnosis, by separating it from serum by a specified column chromatography and affinity chromatography, and providing the gel cataphoresis and dyeing before performing scanning by means of a densitometer.
CONSTITUTION: DNA-binding protein is separated from the serum inspected by the column chromatography using diethylaminoethyl ether (DEAE)-Sephadex and the affinity chromatography using the deoxyribonucleic acid (DNA)-binding carrier. After a more precise separation is done by the gel cataphoresis, scanning is performed by means of a densitometer in order to determine DNA-binding protein of molecular weight approximately 64,000. The microcolumn, filler, various buffer solutions, eluate, precipitant, gel filler, dyeing plate, and reagent for measuring protein which are used in the above method, are made to be a set to provide a quantitative kit.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7661579A JPS561351A (en) | 1979-06-18 | 1979-06-18 | Simple fractionation and determination of serum deoxyribonucleic acid-binding protein closely related to canser by using gel cataphoresis and quantitative kit therefor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7661579A JPS561351A (en) | 1979-06-18 | 1979-06-18 | Simple fractionation and determination of serum deoxyribonucleic acid-binding protein closely related to canser by using gel cataphoresis and quantitative kit therefor |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS561351A true JPS561351A (en) | 1981-01-09 |
Family
ID=13610244
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP7661579A Pending JPS561351A (en) | 1979-06-18 | 1979-06-18 | Simple fractionation and determination of serum deoxyribonucleic acid-binding protein closely related to canser by using gel cataphoresis and quantitative kit therefor |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS561351A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4519763A (en) * | 1982-03-29 | 1985-05-28 | Matsushita Electric Industrial Co., Ltd. | Apparatus for injection compression molding |
US6326136B1 (en) | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
CN103472236A (en) * | 2013-09-11 | 2013-12-25 | 深圳先进技术研究院 | Method for detecting DNA (deoxyribonucleic acid) binding protein |
-
1979
- 1979-06-18 JP JP7661579A patent/JPS561351A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4519763A (en) * | 1982-03-29 | 1985-05-28 | Matsushita Electric Industrial Co., Ltd. | Apparatus for injection compression molding |
US6326136B1 (en) | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
CN103472236A (en) * | 2013-09-11 | 2013-12-25 | 深圳先进技术研究院 | Method for detecting DNA (deoxyribonucleic acid) binding protein |
CN103472236B (en) * | 2013-09-11 | 2015-06-03 | 深圳先进技术研究院 | Method for detecting DNA (deoxyribonucleic acid) binding protein |
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