JPS56133660A - Composition and method for determination of peroxide substance - Google Patents

Composition and method for determination of peroxide substance

Info

Publication number
JPS56133660A
JPS56133660A JP3863080A JP3863080A JPS56133660A JP S56133660 A JPS56133660 A JP S56133660A JP 3863080 A JP3863080 A JP 3863080A JP 3863080 A JP3863080 A JP 3863080A JP S56133660 A JPS56133660 A JP S56133660A
Authority
JP
Japan
Prior art keywords
group
sulfoalkyl
hydrogen atom
lower alkyl
substituent selected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3863080A
Other languages
Japanese (ja)
Inventor
Masayasu Maeda
Yuko Murao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dojin Kagaku Kenkyusho Kk
DOUJIN KAGAKU KENKYUSHO KK
Original Assignee
Dojin Kagaku Kenkyusho Kk
DOUJIN KAGAKU KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dojin Kagaku Kenkyusho Kk, DOUJIN KAGAKU KENKYUSHO KK filed Critical Dojin Kagaku Kenkyusho Kk
Priority to JP3863080A priority Critical patent/JPS56133660A/en
Priority to DE19803037342 priority patent/DE3037342A1/en
Priority to US06/278,321 priority patent/US4396714A/en
Publication of JPS56133660A publication Critical patent/JPS56133660A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

PURPOSE:To obtain a stable measured value by oxidation and condensation with a color former, such as 4-amino antipyrin by using a composition which having as a coloring-matter forming substance an N-sulfoalkyl aniline derivative or its salt. CONSTITUTION:An N-sulfoalkyl aniline derivative shown by the formula (R<1> is a substituent selected among a hydrogen atom, lower alkyl group and sulfoalkyl group of 1-4 carbon number; R2 is a sulfoalkyl group of 1-4 carbon number; R<3> is a substituent selected among a hydrogen atom, alkoxy group, hydroxyl group, and lower alkyl group; and R<4> is a substituent selected among a hydrogen atom, alkoxy group and lower alkyl group) or its salt is dissolved in an acetate buffer solution together with glucose oxidase peroxidase and 4-amino antipyrin to obtain a coloring reagent. Then, the coloring regent is added to blood serum in a test tube for reaction and then the light bsorbance in contrast, to the blank teston regent is measured and contrasted with a calibration line to calculate the concentration of glucose in the serum.
JP3863080A 1980-01-09 1980-03-25 Composition and method for determination of peroxide substance Pending JPS56133660A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP3863080A JPS56133660A (en) 1980-03-25 1980-03-25 Composition and method for determination of peroxide substance
DE19803037342 DE3037342A1 (en) 1980-01-09 1980-10-02 N-SULFOALKYLANILINE DERIVATIVES AND THEIR USE
US06/278,321 US4396714A (en) 1980-01-09 1981-06-29 N-Sulfoalkylaniline derivatives

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3863080A JPS56133660A (en) 1980-03-25 1980-03-25 Composition and method for determination of peroxide substance

Publications (1)

Publication Number Publication Date
JPS56133660A true JPS56133660A (en) 1981-10-19

Family

ID=12530552

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3863080A Pending JPS56133660A (en) 1980-01-09 1980-03-25 Composition and method for determination of peroxide substance

Country Status (1)

Country Link
JP (1) JPS56133660A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57206399A (en) * 1981-06-12 1982-12-17 Shinotesuto Kenkyusho:Kk Enzymic determining method of glucose
JPS5813398A (en) * 1981-06-23 1983-01-25 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Agent and method for detecting hydrogen peroxide, substrate containing same, peroxidase and substance having peroxidase like action

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57206399A (en) * 1981-06-12 1982-12-17 Shinotesuto Kenkyusho:Kk Enzymic determining method of glucose
JPS5813398A (en) * 1981-06-23 1983-01-25 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Agent and method for detecting hydrogen peroxide, substrate containing same, peroxidase and substance having peroxidase like action
JPS6134799B2 (en) * 1981-06-23 1986-08-09 Boehringer Mannheim Gmbh

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