JPS56113289A - Concentration and purification of beta-amylase - Google Patents
Concentration and purification of beta-amylaseInfo
- Publication number
- JPS56113289A JPS56113289A JP1499280A JP1499280A JPS56113289A JP S56113289 A JPS56113289 A JP S56113289A JP 1499280 A JP1499280 A JP 1499280A JP 1499280 A JP1499280 A JP 1499280A JP S56113289 A JPS56113289 A JP S56113289A
- Authority
- JP
- Japan
- Prior art keywords
- amylase
- extract
- purification
- halogen
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To carry out the concentration and purification of β-amylase in high efficiency, by adding a salt of a specific metal and a halogen-free anion to a β-amylase extract liquid, and concentrating the mixture through a membrane.
CONSTITUTION: A raw material such as soybean, malt, sweet potato, etc. is extracted with water or a buffer solution to obtain the extract containing β-amylase. Proteins are removed from the extract by acid precipitation, salting out at a relatively low degree of saturation, addition of an alkaline earth metal, etc. The extract is mixed with ≥0.01% of a salt of a metal selected from Ca, Mg and Al and a halogen-free anion, e.g. CaSO4, magnesium acetate, aluminum borate, etc., adjusted to pH4W6, and concentrated with a membrane, e.g. by ultrafiltration, reverse osmosis, etc. at 50W55°C. By this process, the putrefaction of β-amylase is prevented while keeping the high enzymatic activity.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1499280A JPS56113289A (en) | 1980-02-09 | 1980-02-09 | Concentration and purification of beta-amylase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1499280A JPS56113289A (en) | 1980-02-09 | 1980-02-09 | Concentration and purification of beta-amylase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS56113289A true JPS56113289A (en) | 1981-09-07 |
Family
ID=11876429
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1499280A Pending JPS56113289A (en) | 1980-02-09 | 1980-02-09 | Concentration and purification of beta-amylase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS56113289A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9139856B2 (en) | 2008-03-12 | 2015-09-22 | Tata Chemicals Ltd. | Process for production of galactooligosaccharides (GOS) |
-
1980
- 1980-02-09 JP JP1499280A patent/JPS56113289A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9139856B2 (en) | 2008-03-12 | 2015-09-22 | Tata Chemicals Ltd. | Process for production of galactooligosaccharides (GOS) |
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