JPS56102797A - Reagent for measuring enzyme activity participating in purine metabolism - Google Patents
Reagent for measuring enzyme activity participating in purine metabolismInfo
- Publication number
- JPS56102797A JPS56102797A JP506280A JP506280A JPS56102797A JP S56102797 A JPS56102797 A JP S56102797A JP 506280 A JP506280 A JP 506280A JP 506280 A JP506280 A JP 506280A JP S56102797 A JPS56102797 A JP S56102797A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- reagent
- activity
- enzyme activity
- ada
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To obtain a reagent for measuring purine nucleoside phosphorylase (PNP) activity, adenosine deaminase(ADA) activity, and guanase activity in the blood, in the blood dissolving red corpsucle, and in a culture solution, by combining a substrate solution, coloring solution, enzyme solution, and reaction terminating agent.
CONSTITUTION: For PNP, a substrate solution consists of inosine, sodium azide, and 0.05W2mol buffer solution in pH 7W8, for ADA, it consists of adenosine, sodium, azide, and 0.05W0.2mol buffer solution in pH 7W8, and, for guanase, it consists of guanine, sodium azide, and 0.05W0.2mol buffer solution in pH 6W7. An enzyme solution is a solution of 1.5W4mol xanthine oxidase or xanthine oxidase and prine nucleoside phosphorylase in ammonium sulfate suspension or 40W60wt% glycerol solution. A reaction terminating agent consists of 0.1W0.3mol acitic acid solution, etc.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP506280A JPS6028279B2 (en) | 1980-01-19 | 1980-01-19 | Reagent for measuring enzyme activity involved in purine metabolism |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP506280A JPS6028279B2 (en) | 1980-01-19 | 1980-01-19 | Reagent for measuring enzyme activity involved in purine metabolism |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS56102797A true JPS56102797A (en) | 1981-08-17 |
JPS6028279B2 JPS6028279B2 (en) | 1985-07-03 |
Family
ID=11600901
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP506280A Expired JPS6028279B2 (en) | 1980-01-19 | 1980-01-19 | Reagent for measuring enzyme activity involved in purine metabolism |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6028279B2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60176600A (en) * | 1984-02-24 | 1985-09-10 | Fujimoto Rinshiyou Kensa Kenkyusho:Kk | Method for measuring activity of guanase |
JPS6117519A (en) * | 1984-07-04 | 1986-01-25 | Maruho Kk | Blood selection for transfusion |
-
1980
- 1980-01-19 JP JP506280A patent/JPS6028279B2/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60176600A (en) * | 1984-02-24 | 1985-09-10 | Fujimoto Rinshiyou Kensa Kenkyusho:Kk | Method for measuring activity of guanase |
JPS6117519A (en) * | 1984-07-04 | 1986-01-25 | Maruho Kk | Blood selection for transfusion |
Also Published As
Publication number | Publication date |
---|---|
JPS6028279B2 (en) | 1985-07-03 |
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