JPS5562902A - Separation of endotoxin - Google Patents

Separation of endotoxin

Info

Publication number
JPS5562902A
JPS5562902A JP13703678A JP13703678A JPS5562902A JP S5562902 A JPS5562902 A JP S5562902A JP 13703678 A JP13703678 A JP 13703678A JP 13703678 A JP13703678 A JP 13703678A JP S5562902 A JPS5562902 A JP S5562902A
Authority
JP
Japan
Prior art keywords
endotoxin
protein
metallic ions
chromatography
metal chelate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP13703678A
Other languages
Japanese (ja)
Other versions
JPS6159292B2 (en
Inventor
Kazuo Hosoi
Hitoshi Ozawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Priority to JP13703678A priority Critical patent/JPS5562902A/en
Publication of JPS5562902A publication Critical patent/JPS5562902A/en
Publication of JPS6159292B2 publication Critical patent/JPS6159292B2/ja
Granted legal-status Critical Current

Links

Abstract

PURPOSE: To obtain a protein particularly suitable for injection free from endotoxin, by subjecting a useful protein containing endotoxin to chromatography, using a metal chelate carrier.
CONSTITUTION: A useful protein containing endotoxin is subjected to chromatography using a with a metal chelate carrier having metallic ions combined complex- forming group, e.g. an insoluble carrier comprising iminodiacetic acid-metallic ions combined with epoxy activated sepharose of the formula, to adsorb the imidazole groups or thiol groups in the side chain of the protein on the metallic ions with coordination bonds. the endotoxin passes through the column without adsorption. The desired protein free from the endotoxin can be obtained in high yield by severing the coordination bonds.
COPYRIGHT: (C)1980,JPO&Japio
JP13703678A 1978-11-07 1978-11-07 Separation of endotoxin Granted JPS5562902A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13703678A JPS5562902A (en) 1978-11-07 1978-11-07 Separation of endotoxin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13703678A JPS5562902A (en) 1978-11-07 1978-11-07 Separation of endotoxin

Publications (2)

Publication Number Publication Date
JPS5562902A true JPS5562902A (en) 1980-05-12
JPS6159292B2 JPS6159292B2 (en) 1986-12-16

Family

ID=15189342

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13703678A Granted JPS5562902A (en) 1978-11-07 1978-11-07 Separation of endotoxin

Country Status (1)

Country Link
JP (1) JPS5562902A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61148197A (en) * 1984-12-05 1986-07-05 イ−ライ・リリ−・アンド・カンパニ− Improvement in protein purification
WO1994003626A1 (en) * 1992-07-31 1994-02-17 The Green Cross Corporation Method of highly purifying human serum albumin
US10758623B2 (en) 2013-12-09 2020-09-01 Durect Corporation Pharmaceutically active agent complexes, polymer complexes, and compositions and methods involving the same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61148197A (en) * 1984-12-05 1986-07-05 イ−ライ・リリ−・アンド・カンパニ− Improvement in protein purification
WO1994003626A1 (en) * 1992-07-31 1994-02-17 The Green Cross Corporation Method of highly purifying human serum albumin
US10758623B2 (en) 2013-12-09 2020-09-01 Durect Corporation Pharmaceutically active agent complexes, polymer complexes, and compositions and methods involving the same

Also Published As

Publication number Publication date
JPS6159292B2 (en) 1986-12-16

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