JPS5536759A - Measuring method of kallikrein in human urine - Google Patents

Measuring method of kallikrein in human urine

Info

Publication number
JPS5536759A
JPS5536759A JP10990478A JP10990478A JPS5536759A JP S5536759 A JPS5536759 A JP S5536759A JP 10990478 A JP10990478 A JP 10990478A JP 10990478 A JP10990478 A JP 10990478A JP S5536759 A JPS5536759 A JP S5536759A
Authority
JP
Japan
Prior art keywords
human urine
methylcoumarin
prolyl
arginine
amide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10990478A
Other languages
Japanese (ja)
Other versions
JPS6130559B2 (en
Inventor
Sadaaki Iwanaga
Hisao Kato
Shunpei Sakakibara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP10990478A priority Critical patent/JPS5536759A/en
Publication of JPS5536759A publication Critical patent/JPS5536759A/en
Publication of JPS6130559B2 publication Critical patent/JPS6130559B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To measure a very small amount of kallikrein contained in human urine in high sensitivity by adding prolyl-phenyl-alanyl-arginine-4-methylcoumarin-amide into human urine whose pH is adjusted in a specified range, and measuring the product by a fluorescent method.
CONSTITUTION: An enzyme reaction is made by contacting prolyl-phenylalanyl- arginine-4-methylcoumarin-amide with human urine whose pH range is adjusted from 8 to 12 and 7-amino-4-methyl-coumarin is produced, then, it is measured by a fluorescent method. For example, 0.5ml of 0.1-M tris-HCl buffer solution (pH 8.0) containing 0.15M of NaCl is mixed with 5W100μl of human urine at 37°. After 5 minutes, 5μl of 10-mM L-prolyl-L-phenylalanyl-L-arginine-4-methylcoumarin-amide is added, thus 7-amino-4-methylcoumarin is produced, and its fluorescent intensity at 460nm is measured.
COPYRIGHT: (C)1980,JPO&Japio
JP10990478A 1978-09-07 1978-09-07 Measuring method of kallikrein in human urine Granted JPS5536759A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10990478A JPS5536759A (en) 1978-09-07 1978-09-07 Measuring method of kallikrein in human urine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10990478A JPS5536759A (en) 1978-09-07 1978-09-07 Measuring method of kallikrein in human urine

Publications (2)

Publication Number Publication Date
JPS5536759A true JPS5536759A (en) 1980-03-14
JPS6130559B2 JPS6130559B2 (en) 1986-07-14

Family

ID=14522095

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10990478A Granted JPS5536759A (en) 1978-09-07 1978-09-07 Measuring method of kallikrein in human urine

Country Status (1)

Country Link
JP (1) JPS5536759A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335203A (en) * 1980-07-10 1982-06-15 The Regents Of The University Of California Method for identifying potential contrast media reactors
JPS5843798A (en) * 1981-09-09 1983-03-14 Mamoru Sugiura Reagent for determining activity of kallikrein in human urine
JPS59104555A (en) * 1982-11-08 1984-06-16 アボツト・ラボラトリ−ズ Method of measuring terminal deoxynucleotidyltransferase

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0382648A (en) * 1989-08-28 1991-04-08 Michiharu Saji Automatic seat belt

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335203A (en) * 1980-07-10 1982-06-15 The Regents Of The University Of California Method for identifying potential contrast media reactors
JPS5843798A (en) * 1981-09-09 1983-03-14 Mamoru Sugiura Reagent for determining activity of kallikrein in human urine
JPS59104555A (en) * 1982-11-08 1984-06-16 アボツト・ラボラトリ−ズ Method of measuring terminal deoxynucleotidyltransferase

Also Published As

Publication number Publication date
JPS6130559B2 (en) 1986-07-14

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