JPS5534047A - Preparation of alpha-amylase - Google Patents
Preparation of alpha-amylaseInfo
- Publication number
- JPS5534047A JPS5534047A JP10661478A JP10661478A JPS5534047A JP S5534047 A JPS5534047 A JP S5534047A JP 10661478 A JP10661478 A JP 10661478A JP 10661478 A JP10661478 A JP 10661478A JP S5534047 A JPS5534047 A JP S5534047A
- Authority
- JP
- Japan
- Prior art keywords
- amylase
- cycloserine
- bacteria
- bacillus subtilis
- culturing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To prepare a high-titer α-amylase, by culturing a cycloserine-resistant strain of α-amylase-producing bacteria.
CONSTITUTION: A non-cycloserine-resistant, α-amylase-producing strain of Bacillus subtilis is treated with a solution containing 150μg/ml of N-methyl-N'-nitro-N- nitrosoguanidine at 37°C for 15 minutes to induce the mutaion. The mutant thus induced is cultured in a plate medium containing 200μg/ml of cycloserine for 2-3 days, and the cycloserine-resistant strain of the bacteria such as Bacillus subtilis CS108, Bacillus subtilis 630T2, etc. is separated from the large colony by fishing. α- Amylase is prepared by culturing the bacteria thus obtained as α-anylase-producing bacteria.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10661478A JPS5534047A (en) | 1978-08-31 | 1978-08-31 | Preparation of alpha-amylase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10661478A JPS5534047A (en) | 1978-08-31 | 1978-08-31 | Preparation of alpha-amylase |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12444579A Division JPS5548387A (en) | 1979-09-27 | 1979-09-27 | Microorganism having cycloserine resistance |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5534047A true JPS5534047A (en) | 1980-03-10 |
JPS6243677B2 JPS6243677B2 (en) | 1987-09-16 |
Family
ID=14437990
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10661478A Granted JPS5534047A (en) | 1978-08-31 | 1978-08-31 | Preparation of alpha-amylase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5534047A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01222771A (en) * | 1987-03-30 | 1989-09-06 | Kao Corp | Mutant strain resistant to cell membrane synthesis inhibitor |
EP0337009A2 (en) * | 1988-03-30 | 1989-10-18 | Kao Corporation | Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same |
JPH038106U (en) * | 1989-06-08 | 1991-01-25 |
-
1978
- 1978-08-31 JP JP10661478A patent/JPS5534047A/en active Granted
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01222771A (en) * | 1987-03-30 | 1989-09-06 | Kao Corp | Mutant strain resistant to cell membrane synthesis inhibitor |
JP2879682B2 (en) * | 1987-03-30 | 1999-04-05 | 花王株式会社 | Vancomycin or ristocetin-resistant mutants and their preparation |
EP0337009A2 (en) * | 1988-03-30 | 1989-10-18 | Kao Corporation | Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same |
US5079154A (en) * | 1988-03-30 | 1992-01-07 | Kao Corporation | Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same |
JPH038106U (en) * | 1989-06-08 | 1991-01-25 |
Also Published As
Publication number | Publication date |
---|---|
JPS6243677B2 (en) | 1987-09-16 |
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