JPS5533627A - Quantitization of mono-substituted guanidine - Google Patents
Quantitization of mono-substituted guanidineInfo
- Publication number
- JPS5533627A JPS5533627A JP10629578A JP10629578A JPS5533627A JP S5533627 A JPS5533627 A JP S5533627A JP 10629578 A JP10629578 A JP 10629578A JP 10629578 A JP10629578 A JP 10629578A JP S5533627 A JPS5533627 A JP S5533627A
- Authority
- JP
- Japan
- Prior art keywords
- substituted guanidine
- measured
- added
- mono
- benzoin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
PURPOSE:To make it possible to easily and reliably quantitize guanidines by adding benzoin to a sample thereby to effect thermal reaction and by measuring the fluorescent intensity of the reacted mixture. CONSTITUTION:Benzoin, which is dissolved in a solvent such as dimethyl formamide, is added to a sample containing mono-substituted guanidine thereby to effect the thermal reaction in a boiling water bath in the presence of alkali for 40-50 minutes. Preferably, mercaptoethanol is added in the form of its aqueous solution to the reacted mixture, and then the fluorescent intensity is measured. In this method, the maximum wavelengths of excitation and of fluorescence are constant at 320- 328nm and 434-437nm, respectively, and the difference inbetween is so large, e.g., about 110 nm that it can be measured in a visible range in a simple manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10629578A JPS5533627A (en) | 1978-09-01 | 1978-09-01 | Quantitization of mono-substituted guanidine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10629578A JPS5533627A (en) | 1978-09-01 | 1978-09-01 | Quantitization of mono-substituted guanidine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5533627A true JPS5533627A (en) | 1980-03-08 |
| JPS6134615B2 JPS6134615B2 (en) | 1986-08-08 |
Family
ID=14430042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10629578A Granted JPS5533627A (en) | 1978-09-01 | 1978-09-01 | Quantitization of mono-substituted guanidine |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5533627A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020158750A1 (en) | 2019-01-31 | 2020-08-06 | 旭化成株式会社 | Absorbent pad for immunochromatographic diagnosis kit |
| WO2020230826A1 (en) | 2019-05-14 | 2020-11-19 | 旭化成株式会社 | Absorptive pad for immunochromatographic diagnosis kit and immunochromatographic diagnosis kit |
-
1978
- 1978-09-01 JP JP10629578A patent/JPS5533627A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6134615B2 (en) | 1986-08-08 |
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