JPS55304A - Purification of hbs antigen - Google Patents
Purification of hbs antigenInfo
- Publication number
- JPS55304A JPS55304A JP3655578A JP3655578A JPS55304A JP S55304 A JPS55304 A JP S55304A JP 3655578 A JP3655578 A JP 3655578A JP 3655578 A JP3655578 A JP 3655578A JP S55304 A JPS55304 A JP S55304A
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- exchange material
- plasma
- globulin
- sepharose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To prepare HBs antigen which is an antigen of the hepatitis B virus, in high purity and with a small-sized apparatus, by the ion exchange chromatographic treatment of blood plasma or serum.
CONSTITUTION: Blood plasma or blood serum is treated with an anion exchange material (e.g. DEAE-Sepharose CL-6B: product of Pharmacea Co.) to remove the plasma components other than the protein components mainly composed of KBs antigen and γ-globulin. The solution containing the plasma proteins is treated with a cation exchange material (e.g. CM-Sepharose CL-6B) to adsorb and remove the protein components mainly composed of γ-globulin. The purified HBs antigen can be separated from the fraction which is not adsorbed to the ion exchange material. The antigen contains extremely small amount of HB viruses.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3655578A JPS55304A (en) | 1978-03-31 | 1978-03-31 | Purification of hbs antigen |
CA312,397A CA1108051A (en) | 1978-03-31 | 1978-09-29 | Method for purification of hbs antigen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3655578A JPS55304A (en) | 1978-03-31 | 1978-03-31 | Purification of hbs antigen |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55304A true JPS55304A (en) | 1980-01-05 |
JPS6145610B2 JPS6145610B2 (en) | 1986-10-08 |
Family
ID=12472994
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3655578A Granted JPS55304A (en) | 1978-03-31 | 1978-03-31 | Purification of hbs antigen |
Country Status (2)
Country | Link |
---|---|
JP (1) | JPS55304A (en) |
CA (1) | CA1108051A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4599231A (en) * | 1984-03-09 | 1986-07-08 | Scripps Clinic And Research Foundation | Synthetic hepatitis B virus vaccine including both T cell and B cell determinants |
-
1978
- 1978-03-31 JP JP3655578A patent/JPS55304A/en active Granted
- 1978-09-29 CA CA312,397A patent/CA1108051A/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS6145610B2 (en) | 1986-10-08 |
CA1108051A (en) | 1981-09-01 |
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