JPS5517474A - Analysis method for amino acid - Google Patents

Analysis method for amino acid

Info

Publication number
JPS5517474A
JPS5517474A JP9084778A JP9084778A JPS5517474A JP S5517474 A JPS5517474 A JP S5517474A JP 9084778 A JP9084778 A JP 9084778A JP 9084778 A JP9084778 A JP 9084778A JP S5517474 A JPS5517474 A JP S5517474A
Authority
JP
Japan
Prior art keywords
amino acid
column
buffer solution
analysis
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP9084778A
Other languages
Japanese (ja)
Inventor
Yoshio Fujii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP9084778A priority Critical patent/JPS5517474A/en
Publication of JPS5517474A publication Critical patent/JPS5517474A/en
Pending legal-status Critical Current

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  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

PURPOSE: To remove the error due to ammonium plateau, by reproducing column after the end of analysis for the acid or neutral amino acid for the inspected object and by analyzing basic amino acid with the inspected object again the column, in the amino acid analysis in single column.
CONSTITUTION: The inspected objects sampled with the automatic sampler 4 and the buffer solution from the buffer solution groups 1-1 to 1-6 are mixed and fed to the separation column 5, and ninhydrin reagent 12 is mixed at the mixer 7, and each amino acid is detected with photometer 9. In such amino acid automatic analysis, the buffer liquids 1-1 to 1-3 are sequentially selected to the first inspected objects, and the column is reproduced when acid or nutral amino acid group (e.g., asparagine acid to leucine) is analyzed, and basic amino acid group (e.g., thyrosin to arginine) is analyzed by putting in the tested object again while flowing the buffer solution 1-4. With the said method, the interference due to ammonium plateau can be avoided.
COPYRIGHT: (C)1980,JPO&Japio
JP9084778A 1978-07-25 1978-07-25 Analysis method for amino acid Pending JPS5517474A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9084778A JPS5517474A (en) 1978-07-25 1978-07-25 Analysis method for amino acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9084778A JPS5517474A (en) 1978-07-25 1978-07-25 Analysis method for amino acid

Publications (1)

Publication Number Publication Date
JPS5517474A true JPS5517474A (en) 1980-02-06

Family

ID=14009969

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9084778A Pending JPS5517474A (en) 1978-07-25 1978-07-25 Analysis method for amino acid

Country Status (1)

Country Link
JP (1) JPS5517474A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013054742A1 (en) * 2011-10-11 2013-04-18 株式会社日立ハイテクノロジーズ Fluid mixer and fluid mixing method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013054742A1 (en) * 2011-10-11 2013-04-18 株式会社日立ハイテクノロジーズ Fluid mixer and fluid mixing method
JP2013081911A (en) * 2011-10-11 2013-05-09 Hitachi High-Technologies Corp Fluid mixer and fluid mixing method

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