JPS55159158A - Novel reagent for separation of lipoprotein - Google Patents
Novel reagent for separation of lipoproteinInfo
- Publication number
- JPS55159158A JPS55159158A JP6762179A JP6762179A JPS55159158A JP S55159158 A JPS55159158 A JP S55159158A JP 6762179 A JP6762179 A JP 6762179A JP 6762179 A JP6762179 A JP 6762179A JP S55159158 A JPS55159158 A JP S55159158A
- Authority
- JP
- Japan
- Prior art keywords
- lipoprotein
- kinds
- metal ion
- precipitant
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: To separate α, β-lipoprotein securely and promptly, by using a mixture of one or more kinds of polyanion, bivalent metal ion, alkali metal ion, and NH4 ion, as precipitant, and adding one or more kinds of specified precipitation aids.
CONSTITUTION: In order to separate lipoprotein from serum or the like in diagnosis of arteriosclerosis, coronary heart disease and other symptoms, a mixture of polyanion such as heparin, and bivalent metal ion such as Mn and Ca, or the solution added with one or more kinds of alkali metal ion and NH4 + is used as precipitant. Also, as precipitation aid, one or more kinds of alumina, aluminum silicate, fibrin, fibrinogen, and albumin are used. Said precipitant and precipitation aid are separately added or added as mixture to a sample fluid such as serum. Then, the sample is centrifuged, and cholesterols in α-lipoprotein in the supernatant and β-lipoprotein in the precipitate are measured. Thus, the lipoprotein of low molecular weight, which has been conventionally difficult to separate, may be completely separated, which, therefore, contributes greatly to diagnosis.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6762179A JPS55159158A (en) | 1979-05-31 | 1979-05-31 | Novel reagent for separation of lipoprotein |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6762179A JPS55159158A (en) | 1979-05-31 | 1979-05-31 | Novel reagent for separation of lipoprotein |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55159158A true JPS55159158A (en) | 1980-12-11 |
JPS6311628B2 JPS6311628B2 (en) | 1988-03-15 |
Family
ID=13350221
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6762179A Granted JPS55159158A (en) | 1979-05-31 | 1979-05-31 | Novel reagent for separation of lipoprotein |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55159158A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4486531A (en) * | 1980-02-29 | 1984-12-04 | Boehringer Mannheim Gmbh | Method and reagent for the determination of β-lipoprotein |
JPS63243878A (en) * | 1987-03-31 | 1988-10-11 | Nippon Koutai Kenkyusho:Kk | Detection of lipid metabolism abnormality |
JPH0216455A (en) * | 1981-09-10 | 1990-01-19 | Intermedicat Gmbh | Selective settlement of low-density lipoprotein outside body |
JPH05322884A (en) * | 1992-05-22 | 1993-12-07 | Inax Corp | Method for measuring bio-component |
EP0602455A2 (en) * | 1992-12-14 | 1994-06-22 | Bayer Corporation | Method for the separation of high density lipoprotein from blood samples |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0398039A (en) * | 1989-09-12 | 1991-04-23 | Toppan Printing Co Ltd | Reflection type screen |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5225456A (en) * | 1975-08-20 | 1977-02-25 | Mitsubishi Heavy Ind Ltd | Method of cropping useful matters from cohesion deposit sludg e of waste water |
JPS55116261A (en) * | 1978-12-15 | 1980-09-06 | Beckman Instruments Inc | Reagent for precipitating nonnhighhdensity lipoprotein |
-
1979
- 1979-05-31 JP JP6762179A patent/JPS55159158A/en active Granted
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5225456A (en) * | 1975-08-20 | 1977-02-25 | Mitsubishi Heavy Ind Ltd | Method of cropping useful matters from cohesion deposit sludg e of waste water |
JPS55116261A (en) * | 1978-12-15 | 1980-09-06 | Beckman Instruments Inc | Reagent for precipitating nonnhighhdensity lipoprotein |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4486531A (en) * | 1980-02-29 | 1984-12-04 | Boehringer Mannheim Gmbh | Method and reagent for the determination of β-lipoprotein |
JPH0216455A (en) * | 1981-09-10 | 1990-01-19 | Intermedicat Gmbh | Selective settlement of low-density lipoprotein outside body |
JPH0340342B2 (en) * | 1981-09-10 | 1991-06-18 | ||
JPS63243878A (en) * | 1987-03-31 | 1988-10-11 | Nippon Koutai Kenkyusho:Kk | Detection of lipid metabolism abnormality |
JPH05322884A (en) * | 1992-05-22 | 1993-12-07 | Inax Corp | Method for measuring bio-component |
JP2673980B2 (en) * | 1992-05-22 | 1997-11-05 | 株式会社イナックス | Measuring method of biological components |
EP0602455A2 (en) * | 1992-12-14 | 1994-06-22 | Bayer Corporation | Method for the separation of high density lipoprotein from blood samples |
EP0602455A3 (en) * | 1992-12-14 | 1995-08-16 | Miles Inc | Method for the separation of high density lipoprotein from blood samples. |
Also Published As
Publication number | Publication date |
---|---|
JPS6311628B2 (en) | 1988-03-15 |
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