JPS55131389A - Purification of deoxyribonuclease i - Google Patents

Purification of deoxyribonuclease i

Info

Publication number
JPS55131389A
JPS55131389A JP3843879A JP3843879A JPS55131389A JP S55131389 A JPS55131389 A JP S55131389A JP 3843879 A JP3843879 A JP 3843879A JP 3843879 A JP3843879 A JP 3843879A JP S55131389 A JPS55131389 A JP S55131389A
Authority
JP
Japan
Prior art keywords
deoxyribonuclease
deoxyribonucleic acid
extracted
column
mammalian
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP3843879A
Other languages
Japanese (ja)
Other versions
JPS6317436B2 (en
Inventor
Hideyuki Tanaka
Hideyuki Hasegawa
Takeshi Matsuo
Buichi Horio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Pharmaceutical Co Ltd filed Critical Amano Pharmaceutical Co Ltd
Priority to JP3843879A priority Critical patent/JPS55131389A/en
Publication of JPS55131389A publication Critical patent/JPS55131389A/en
Publication of JPS6317436B2 publication Critical patent/JPS6317436B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To purify the title enzyme in high purity with ease useful as reagents and drugs, by adsorbing deoxyribonuclease I in an extracted liquid of mammalian pancreas on a deoxyribonucleic acid fixed agarose, followed by its dissolution.
CONSTITUTION: Mammalian pancrease are minced, suspended in water, and extracted with an aqueous solution of acetic acid, etc. to give an extract-containing liquid. A deoxyribonucleic acid fixed agarose, obtained by fixing deoxyribonucleic acid on cyanogen bromide activated Sepharose, is filled in a column which is previously adjusted to a pH of about 4W5 with a buffer solution of citric acid. The pancreas extracted liquid is added to the column and deoxyribonuclease I is adsorbed selectively. The ionic strength is raised by the use of a potassium chloride solution to dissolve deoxyribonuclease I.
COPYRIGHT: (C)1980,JPO&Japio
JP3843879A 1979-04-02 1979-04-02 Purification of deoxyribonuclease i Granted JPS55131389A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3843879A JPS55131389A (en) 1979-04-02 1979-04-02 Purification of deoxyribonuclease i

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3843879A JPS55131389A (en) 1979-04-02 1979-04-02 Purification of deoxyribonuclease i

Publications (2)

Publication Number Publication Date
JPS55131389A true JPS55131389A (en) 1980-10-13
JPS6317436B2 JPS6317436B2 (en) 1988-04-13

Family

ID=12525299

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3843879A Granted JPS55131389A (en) 1979-04-02 1979-04-02 Purification of deoxyribonuclease i

Country Status (1)

Country Link
JP (1) JPS55131389A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0853121A3 (en) * 1988-12-23 1998-08-05 Genentech, Inc. Human DNase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0853121A3 (en) * 1988-12-23 1998-08-05 Genentech, Inc. Human DNase

Also Published As

Publication number Publication date
JPS6317436B2 (en) 1988-04-13

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