JPS55131389A - Purification of deoxyribonuclease i - Google Patents
Purification of deoxyribonuclease iInfo
- Publication number
- JPS55131389A JPS55131389A JP3843879A JP3843879A JPS55131389A JP S55131389 A JPS55131389 A JP S55131389A JP 3843879 A JP3843879 A JP 3843879A JP 3843879 A JP3843879 A JP 3843879A JP S55131389 A JPS55131389 A JP S55131389A
- Authority
- JP
- Japan
- Prior art keywords
- deoxyribonuclease
- deoxyribonucleic acid
- extracted
- column
- mammalian
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To purify the title enzyme in high purity with ease useful as reagents and drugs, by adsorbing deoxyribonuclease I in an extracted liquid of mammalian pancreas on a deoxyribonucleic acid fixed agarose, followed by its dissolution.
CONSTITUTION: Mammalian pancrease are minced, suspended in water, and extracted with an aqueous solution of acetic acid, etc. to give an extract-containing liquid. A deoxyribonucleic acid fixed agarose, obtained by fixing deoxyribonucleic acid on cyanogen bromide activated Sepharose, is filled in a column which is previously adjusted to a pH of about 4W5 with a buffer solution of citric acid. The pancreas extracted liquid is added to the column and deoxyribonuclease I is adsorbed selectively. The ionic strength is raised by the use of a potassium chloride solution to dissolve deoxyribonuclease I.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3843879A JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3843879A JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55131389A true JPS55131389A (en) | 1980-10-13 |
JPS6317436B2 JPS6317436B2 (en) | 1988-04-13 |
Family
ID=12525299
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3843879A Granted JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55131389A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0853121A3 (en) * | 1988-12-23 | 1998-08-05 | Genentech, Inc. | Human DNase |
-
1979
- 1979-04-02 JP JP3843879A patent/JPS55131389A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0853121A3 (en) * | 1988-12-23 | 1998-08-05 | Genentech, Inc. | Human DNase |
Also Published As
Publication number | Publication date |
---|---|
JPS6317436B2 (en) | 1988-04-13 |
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