JPS5489011A - Preparation of interferon - Google Patents
Preparation of interferonInfo
- Publication number
- JPS5489011A JPS5489011A JP15601977A JP15601977A JPS5489011A JP S5489011 A JPS5489011 A JP S5489011A JP 15601977 A JP15601977 A JP 15601977A JP 15601977 A JP15601977 A JP 15601977A JP S5489011 A JPS5489011 A JP S5489011A
- Authority
- JP
- Japan
- Prior art keywords
- interferon
- human
- pref
- originated
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To recover and purify human interferon, easily in an industrial scale, in high yield, by adsorbing a crude solution of interferon produced from human-originated cells with a strong acidic cation exchange material, and subsequently, fractionating with a gel filter.
CONSTITUTION: A curde interferon solution containing interferon induced or produced from human-originated interferon-producing cells, pref. human leukocytes, cultured human lymphoblast cells (esp. Namalwa strain), etc., is brought into contact with a strong acidic cation exchange material having a sulfonic acid group, pref. sulfopropyl group, as an ion exchange group. The adsorbed interferon is desorbed with an aqueous solution having a pH of 5.5-9.0, pref. 6-8, and franctionated with a gel filter for a polymer having a molecular weight of 20,000-30,000, to obtain the pure interferon. The recovery of interferon is ≥ 80% by this process, and the interferon obtained is free of virus-originated nucleic acids, pyrogenic substances, inflammatory substances, etc., and has a specific activity of more than 800 times that of the crude interferon solution.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15601977A JPS5489011A (en) | 1977-12-24 | 1977-12-24 | Preparation of interferon |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15601977A JPS5489011A (en) | 1977-12-24 | 1977-12-24 | Preparation of interferon |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5489011A true JPS5489011A (en) | 1979-07-14 |
JPS574236B2 JPS574236B2 (en) | 1982-01-25 |
Family
ID=15618528
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP15601977A Granted JPS5489011A (en) | 1977-12-24 | 1977-12-24 | Preparation of interferon |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5489011A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1310559A1 (en) * | 2001-11-08 | 2003-05-14 | International Centre for Genetic Engineering and Biotechnology | Process for the production of alpha interferon of therapeutical degree |
KR100374307B1 (en) * | 1995-07-21 | 2003-07-07 | 주식회사 엘지생명과학 | A purification method of gamma interferon from recombinant yeast |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5134442A (en) * | 1974-09-18 | 1976-03-24 | Matsushita Electric Ind Co Ltd | YUWAKASHIKI NOTOKETSUBOSHISOCHI |
-
1977
- 1977-12-24 JP JP15601977A patent/JPS5489011A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5134442A (en) * | 1974-09-18 | 1976-03-24 | Matsushita Electric Ind Co Ltd | YUWAKASHIKI NOTOKETSUBOSHISOCHI |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100374307B1 (en) * | 1995-07-21 | 2003-07-07 | 주식회사 엘지생명과학 | A purification method of gamma interferon from recombinant yeast |
EP1310559A1 (en) * | 2001-11-08 | 2003-05-14 | International Centre for Genetic Engineering and Biotechnology | Process for the production of alpha interferon of therapeutical degree |
Also Published As
Publication number | Publication date |
---|---|
JPS574236B2 (en) | 1982-01-25 |
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