JPS5489011A - Preparation of interferon - Google Patents

Preparation of interferon

Info

Publication number
JPS5489011A
JPS5489011A JP15601977A JP15601977A JPS5489011A JP S5489011 A JPS5489011 A JP S5489011A JP 15601977 A JP15601977 A JP 15601977A JP 15601977 A JP15601977 A JP 15601977A JP S5489011 A JPS5489011 A JP S5489011A
Authority
JP
Japan
Prior art keywords
interferon
human
pref
originated
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP15601977A
Other languages
Japanese (ja)
Other versions
JPS574236B2 (en
Inventor
Akio Matsuo
Takaaki Fujita
Saburo Hayashi
Yutaka Morise
Tadakazu Suyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Priority to JP15601977A priority Critical patent/JPS5489011A/en
Publication of JPS5489011A publication Critical patent/JPS5489011A/en
Publication of JPS574236B2 publication Critical patent/JPS574236B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To recover and purify human interferon, easily in an industrial scale, in high yield, by adsorbing a crude solution of interferon produced from human-originated cells with a strong acidic cation exchange material, and subsequently, fractionating with a gel filter.
CONSTITUTION: A curde interferon solution containing interferon induced or produced from human-originated interferon-producing cells, pref. human leukocytes, cultured human lymphoblast cells (esp. Namalwa strain), etc., is brought into contact with a strong acidic cation exchange material having a sulfonic acid group, pref. sulfopropyl group, as an ion exchange group. The adsorbed interferon is desorbed with an aqueous solution having a pH of 5.5-9.0, pref. 6-8, and franctionated with a gel filter for a polymer having a molecular weight of 20,000-30,000, to obtain the pure interferon. The recovery of interferon is ≥ 80% by this process, and the interferon obtained is free of virus-originated nucleic acids, pyrogenic substances, inflammatory substances, etc., and has a specific activity of more than 800 times that of the crude interferon solution.
COPYRIGHT: (C)1979,JPO&Japio
JP15601977A 1977-12-24 1977-12-24 Preparation of interferon Granted JPS5489011A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15601977A JPS5489011A (en) 1977-12-24 1977-12-24 Preparation of interferon

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15601977A JPS5489011A (en) 1977-12-24 1977-12-24 Preparation of interferon

Publications (2)

Publication Number Publication Date
JPS5489011A true JPS5489011A (en) 1979-07-14
JPS574236B2 JPS574236B2 (en) 1982-01-25

Family

ID=15618528

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15601977A Granted JPS5489011A (en) 1977-12-24 1977-12-24 Preparation of interferon

Country Status (1)

Country Link
JP (1) JPS5489011A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1310559A1 (en) * 2001-11-08 2003-05-14 International Centre for Genetic Engineering and Biotechnology Process for the production of alpha interferon of therapeutical degree
KR100374307B1 (en) * 1995-07-21 2003-07-07 주식회사 엘지생명과학 A purification method of gamma interferon from recombinant yeast

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5134442A (en) * 1974-09-18 1976-03-24 Matsushita Electric Ind Co Ltd YUWAKASHIKI NOTOKETSUBOSHISOCHI

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5134442A (en) * 1974-09-18 1976-03-24 Matsushita Electric Ind Co Ltd YUWAKASHIKI NOTOKETSUBOSHISOCHI

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100374307B1 (en) * 1995-07-21 2003-07-07 주식회사 엘지생명과학 A purification method of gamma interferon from recombinant yeast
EP1310559A1 (en) * 2001-11-08 2003-05-14 International Centre for Genetic Engineering and Biotechnology Process for the production of alpha interferon of therapeutical degree

Also Published As

Publication number Publication date
JPS574236B2 (en) 1982-01-25

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