JPS5455785A - Preparation of l-malic acid dehydrogenase - Google Patents

Preparation of l-malic acid dehydrogenase

Info

Publication number
JPS5455785A
JPS5455785A JP12184777A JP12184777A JPS5455785A JP S5455785 A JPS5455785 A JP S5455785A JP 12184777 A JP12184777 A JP 12184777A JP 12184777 A JP12184777 A JP 12184777A JP S5455785 A JPS5455785 A JP S5455785A
Authority
JP
Japan
Prior art keywords
mdh
malic acid
culturing
alcaligenes
separated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12184777A
Other languages
Japanese (ja)
Other versions
JPS5817590B2 (en
Inventor
Osamu Terada
Naohiro Yoshikawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Priority to JP52121847A priority Critical patent/JPS5817590B2/en
Publication of JPS5455785A publication Critical patent/JPS5455785A/en
Publication of JPS5817590B2 publication Critical patent/JPS5817590B2/en
Expired legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To prepare high purity L-malic acid dehydrogenase (MDH) having high stability and low Km value to oxalacetic acid, and useful for clinical diagnosis, by culturing MDH-producing bacteria belonging to Alcaligenes or Klebsiella genus.
CONSTITUTION: MDH-producing bacteria belonging to Alcaligenes or Klebsiella genus, such as Alcaligenes faecalis ATCC 8750, Klebsiella pneumoniae L ATCC 8307, are aerobically cultured in a nutrient medium at pH7-8 for 1-2 days under stirring. After culturing, bacterial cells are separated by filtering or centrifuging, and crushed. High purity MDH can be separated from the curshed cells. The production rate of the MDH can be improved by adding an enzyme-inducing material, such as fumaric acid, malic acid, etc., to the culturing medium
COPYRIGHT: (C)1979,JPO&Japio
JP52121847A 1977-10-13 1977-10-13 Method for producing L-malic acid dehydrogenated oxygen Expired JPS5817590B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP52121847A JPS5817590B2 (en) 1977-10-13 1977-10-13 Method for producing L-malic acid dehydrogenated oxygen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP52121847A JPS5817590B2 (en) 1977-10-13 1977-10-13 Method for producing L-malic acid dehydrogenated oxygen

Publications (2)

Publication Number Publication Date
JPS5455785A true JPS5455785A (en) 1979-05-04
JPS5817590B2 JPS5817590B2 (en) 1983-04-08

Family

ID=14821397

Family Applications (1)

Application Number Title Priority Date Filing Date
JP52121847A Expired JPS5817590B2 (en) 1977-10-13 1977-10-13 Method for producing L-malic acid dehydrogenated oxygen

Country Status (1)

Country Link
JP (1) JPS5817590B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156966A (en) * 1989-10-13 1992-10-20 Toyo Jozo Kabushiki Kaisha L-carnitine dehydrogenase and process for its production

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59189189U (en) * 1983-06-02 1984-12-15 株式会社　ユニ・デザイン clock

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156966A (en) * 1989-10-13 1992-10-20 Toyo Jozo Kabushiki Kaisha L-carnitine dehydrogenase and process for its production

Also Published As

Publication number Publication date
JPS5817590B2 (en) 1983-04-08

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