JPS54105291A - Preparation of cholesterol oxydase - Google Patents

Preparation of cholesterol oxydase

Info

Publication number
JPS54105291A
JPS54105291A JP1073578A JP1073578A JPS54105291A JP S54105291 A JPS54105291 A JP S54105291A JP 1073578 A JP1073578 A JP 1073578A JP 1073578 A JP1073578 A JP 1073578A JP S54105291 A JPS54105291 A JP S54105291A
Authority
JP
Japan
Prior art keywords
medium
cholesterol
oxydase
agrocybe
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1073578A
Other languages
Japanese (ja)
Other versions
JPS5646758B2 (en
Inventor
Osamu Matsui
Kazuo Nakajima
Tsutomu Taniguchi
Akira Obayashi
Osamu Tanabe
Hideaki Yamada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Shuzo Co Ltd
Original Assignee
Takara Shuzo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Shuzo Co Ltd filed Critical Takara Shuzo Co Ltd
Priority to JP1073578A priority Critical patent/JPS54105291A/en
Publication of JPS54105291A publication Critical patent/JPS54105291A/en
Publication of JPS5646758B2 publication Critical patent/JPS5646758B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To provide a specific strain which produces extremely active chlesterol oxydase(useful for the determination of cholesterol) non-adaptively, in a cultivaion medium.
CONSTITUTION: Basidiomycetes belonging to Agrocybe genus (e.g. Agrocybe pediades K-1) is cultured in a medium (containing carbon sources such as starch, glucose, etc.; nitrogen sources such as enzyme extract, peptone, etc.; inorganic materials such as phosphates, potassium salts, etc.; and vitamins.) at 20W35°C and pH4W7 for 2W7 days, and the desired cholesterol oxidase is separated from the medium by precipitation, etc.
COPYRIGHT: (C)1979,JPO&Japio
JP1073578A 1978-02-01 1978-02-01 Preparation of cholesterol oxydase Granted JPS54105291A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1073578A JPS54105291A (en) 1978-02-01 1978-02-01 Preparation of cholesterol oxydase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1073578A JPS54105291A (en) 1978-02-01 1978-02-01 Preparation of cholesterol oxydase

Publications (2)

Publication Number Publication Date
JPS54105291A true JPS54105291A (en) 1979-08-18
JPS5646758B2 JPS5646758B2 (en) 1981-11-05

Family

ID=11758541

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1073578A Granted JPS54105291A (en) 1978-02-01 1978-02-01 Preparation of cholesterol oxydase

Country Status (1)

Country Link
JP (1) JPS54105291A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2497228A1 (en) * 1980-12-26 1982-07-02 Takara Shuzo Co PROCESS FOR PRODUCING A CHOLESTEROLOXIDASE AND PRODUCT OBTAINED

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2497228A1 (en) * 1980-12-26 1982-07-02 Takara Shuzo Co PROCESS FOR PRODUCING A CHOLESTEROLOXIDASE AND PRODUCT OBTAINED

Also Published As

Publication number Publication date
JPS5646758B2 (en) 1981-11-05

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