JPH1176203A - Stable isotope exhalation gas collecting method, and device to be used for the same - Google Patents
Stable isotope exhalation gas collecting method, and device to be used for the sameInfo
- Publication number
- JPH1176203A JPH1176203A JP9245242A JP24524297A JPH1176203A JP H1176203 A JPH1176203 A JP H1176203A JP 9245242 A JP9245242 A JP 9245242A JP 24524297 A JP24524297 A JP 24524297A JP H1176203 A JPH1176203 A JP H1176203A
- Authority
- JP
- Japan
- Prior art keywords
- gas
- bag
- stable isotope
- exhaled
- exhalation gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、安定同位体呼気ガ
ス採取方法およびそれに使用するための装置に関するも
のであり、さらに詳しくは本発明は、第３相の肺胞気の
みを採取することができるために、正確な安定同位体比
の測定が可能になる安定同位体呼気ガス採取方法および
それに使用するための装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for collecting stable isotope expiratory gas and an apparatus for use in the method, and more particularly, the present invention can collect only phase 3 alveolar air. Therefore, the present invention relates to a stable isotope exhaled gas sampling method capable of accurately measuring a stable isotope ratio, and an apparatus used for the method.
【０００２】[0002]
【従来の技術】従来、呼気の安定同位体比の測定方法
は、安定同位体である13Ｃをラベルとして使用し、これ
を経口投与する前後において、それぞれの呼気を呼出さ
せ呼気ガスをインビボから直接、質量分析システムで分
析し、呼気ガスの同位体比（13ＣＯ2／12ＣＯ2）を算出
していた。2. Description of the Related Art Conventionally, a method for measuring the stable isotope ratio of exhaled breath uses a stable isotope, 13 C, as a label, and exhales each exhaled gas before and after oral administration of the exhaled gas to in vivo. directly analyzed by mass spectrometry system, was calculated isotope exhaled gas (13 CO 2/12 CO 2 ).
【０００３】[0003]
【発明が解決しようとする課題】従来の測定方法では、
安定同位体である13Ｃラベルを経口投与する前後におい
て、それぞれの呼気を呼出させ呼気ガスをインビボから
直接、質量分析システムで分析し、呼気ガスの同位体比
（13ＣＯ2／12ＣＯ2）を算出していたため、質量分析シ
ステムを有していない病院や検査機関では検査や測定が
できなかった。In the conventional measuring method,
Before and after oral administration of 13 C label is a stable isotope, directly exhaled gas from in vivo was called each breath, and analyzed by mass spectrometry system, isotope expired gas (13 CO 2/12 CO 2 ) Therefore, examinations and measurements could not be performed at hospitals and laboratories that do not have mass spectrometry systems.
【０００４】また、バッグに呼気を採取する場合、息を
吐出する初期の段階では肺胞でガス交換を行った肺胞気
ではなく、空気そのものが吐出されるため、呼気中に含
まれる空気と炭酸ガスとが混合されてバッグに呼気ガス
が採取されるため測定に誤差を生じていた。[0004] When exhaled air is collected in a bag, the air itself is exhaled at the initial stage of exhalation, instead of the alveolus gas exchanged in the alveoli. Since the gas and the gas were mixed and the exhaled gas was collected in the bag, an error occurred in the measurement.
【０００５】本発明は、以上のような課題を解決するた
めになされたもので、とくに、呼気中に含まれる空気と
炭酸ガスとが混合されて呼気ガスが採取されないよう
に、肺胞気のみを採取し、これを質量分析システムを有
している機関に持ち込むことで、質量分析システムを有
していない病院や検査機関でも検査や測定結果を得るこ
とができるようにした安定同位体呼気ガス採取方法およ
びそのための装置を提供することを目的とする。SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems. In particular, only the alveolar air is collected so that the air contained in the exhaled air and the carbon dioxide gas are mixed and the exhaled gas is not collected. Stable isotope exhaled gas sampling that can be collected and taken to an organization that has a mass spectrometry system so that tests and measurement results can be obtained even at hospitals and laboratories that do not have a mass spectrometry system It is an object to provide a method and an apparatus therefor.
【０００６】[0006]
【課題を解決するための手段】本発明者は鋭意検討の結
果、上記のような従来の課題を解決することができた。
すなわち本発明は、安定同位体を投与した被験者からの
呼気ガスを、マウスピースを通じて閉鎖系で密封可能な
弾性バッグに直接的に採取し、前記弾性バッグに１回換
気量の１／３〜１／２あるいはそれ以上の呼気ガスが採
取された後に、前記被験者からの呼気ガスを、圧力バル
ブと呼気ガスを下流方向にのみ流出することのできるチ
ェックバルブとを介して閉鎖系で密封可能な呼気ガス採
取バッグにさらに採取することを特徴とする安定同位体
呼気ガス採取方法を提供するものである。As a result of intensive studies, the present inventor was able to solve the above-mentioned conventional problems.
That is, according to the present invention, exhaled gas from a subject to which a stable isotope has been administered is directly collected through a mouthpiece into an elastic bag that can be sealed in a closed system, and the elastic bag is used to collect 1/3 to 1/3 of the tidal volume. / 2 or more of the exhaled gas is collected, the exhaled gas from the subject can be sealed in a closed system via a pressure valve and a check valve which allows the exhaled gas to flow only downstream. It is intended to provide a method for collecting stable isotope exhaled gas, further comprising collecting the gas in a gas collection bag.
【０００７】また本発明は、マウスピースを取り付ける
ためのマウスピース取り付け口と、前記マウスピース取
り付け口に接続された弾性バッグと、前記弾性バッグと
接続された呼気ガス採取バッグと、前記弾性バッグと前
記呼気ガス採取バッグとの間に設けられた、前記弾性バ
ッグ中の圧力が設定値に到達したときに開放可能な圧力
バルブと、前記圧力バルブと前記呼気ガス採取バッグと
の間に設けられた、呼気ガスを下流方向にのみ流出する
ことのできるチェックバルブと、を備えてなる安定同位
体呼気ガス採取装置を提供するものである。を提供する
ものであるThe present invention also provides a mouthpiece mounting opening for mounting a mouthpiece, an elastic bag connected to the mouthpiece mounting opening, a breath gas collection bag connected to the elastic bag, and an elastic bag. A pressure valve provided between the exhalation gas collection bag and openable when the pressure in the elastic bag reaches a set value, and provided between the pressure valve and the exhalation gas collection bag. And a check valve capable of allowing the exhaled gas to flow only in the downstream direction. Is to provide
【０００８】[0008]
【発明の実施の形態】以下、図面を参照しながら本発明
による安定同位体呼気ガス採取方法およびそのための装
置の好適な実施の形態について説明する。図１は本発明
に係る安定同位体呼気ガス採取装置の一例を示す構成図
である。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of a method for collecting stable isotope breath gas and an apparatus therefor according to the present invention will be described below with reference to the drawings. FIG. 1 is a configuration diagram showing an example of a stable isotope expiratory gas sampling device according to the present invention.
【０００９】図１において、符号１で示されるものは、
マウスピース取り付け口であり、２は弾性バッグ、３は
圧力バルブ、４および６はチェックバルブ、５は呼気ガ
ス採取バッグ、７は吸引装置、８は吐出バルブである。In FIG. 1, what is indicated by reference numeral 1 is
Reference numeral 2 denotes an elastic bag, reference numeral 2 denotes an elastic bag, reference numeral 3 denotes a pressure valve, reference numerals 4 and 6 denote check valves, reference numeral 5 denotes an exhalation gas collection bag, reference numeral 7 denotes a suction device, and reference numeral 8 denotes a discharge valve.
【００１０】図１に示すように、安定同位体13Ｃ投与後
において被験者にマウスピース取り付け口１に取り付け
たマウスピースをくわえさせ、１回換気量の約１／３〜
１／２程度もしくはそれ以上の容量が弾性バッグ２に満
たされた時点で圧力バルブ３が開くように設定しておき
１回呼出させる。このとき前記弾性バッグ２が呼気で満
たされて圧力が上昇し圧力バルブ３が開く点の圧力に達
すると前記圧力バルブ３が開き、ガスが呼気ガス採取バ
ッグ５方向にのみ流出することが可能であるチェックバ
ルブ４を流れ、前記呼気ガス採取バッグ５に図２で示す
第３相の肺胞気のみが採取される。この呼気ガス採取バ
ッグ５に採取した呼気ガスを、質量分析システムを有し
ている病院や検査機関に渡し、同位体比の検査や測定を
行う。As shown in FIG. 1, after the administration of the stable isotope 13 C, the subject holds the mouthpiece attached to the mouthpiece attachment port 1 by about 1/3 of the tidal volume.
The pressure valve 3 is set so as to open when the capacity of the elastic bag 2 is about 1/2 or more, and is called once. At this time, when the elastic bag 2 is filled with exhalation and the pressure rises and reaches the pressure at which the pressure valve 3 opens, the pressure valve 3 opens and gas can flow only in the direction of the exhalation gas collection bag 5. After flowing through a certain check valve 4, only the third-phase alveolar air shown in FIG. The exhaled gas collected in the exhaled gas collection bag 5 is transferred to a hospital or an inspection organization having a mass spectrometry system, and the isotope ratio is inspected or measured.
【００１１】なお、前記圧力バルブ３の代わりに電磁バ
ルブを用い、前記弾性バッグ２に圧力センサーを設け、
ある設定圧力になったとき圧力センサーが働き、その信
号において電磁バルブを開閉してもよい。An electromagnetic valve is used in place of the pressure valve 3 and a pressure sensor is provided on the elastic bag 2.
The pressure sensor may operate when a certain set pressure is reached, and the electromagnetic valve may be opened or closed based on the signal.
【００１２】またチェックバルブ６は、呼気ガス採取バ
ッグ５中の空気や呼気ガスを吸引装置７等で吸引する方
向にのみガスを流すようになっている。チェックバルブ
６の後段には、吸引装置７が設けられ、これによって、
呼気ガス採取バッグ５中の呼気ガスを採取することがで
きる。さらに吸引装置７は、呼気ガスを採取する前等に
呼気ガス採取バッグ５内の空気やガスを全て抜いてしま
うとき等にも利用できる。The check valve 6 allows the gas to flow only in the direction in which the air or exhaled gas in the exhaled gas collection bag 5 is sucked by the suction device 7 or the like. A suction device 7 is provided downstream of the check valve 6, whereby
The exhaled gas in the exhaled gas collection bag 5 can be collected. Furthermore, the suction device 7 can also be used when all the air and gas in the breath gas collection bag 5 are removed before collecting the breath gas or the like.
【００１３】吐出バルブ８は、前記弾性バッグ２中に貯
留しているガスを抜くときなどに開放する。The discharge valve 8 is opened when the gas stored in the elastic bag 2 is discharged.
【００１４】第３相の肺胞気の呼気ガスは、呼気ガス採
取バッグ５中から上記のように吸引装置７によって吸引
して設置した容器（図示せず）に採取することもできる
が、呼気ガス採取バッグ５を取り外し可能に設置してお
き、第３相の肺胞気の呼気ガスをそこに採取した後に、
呼気ガス採取バッグ５を取り外し、質量分析システムを
有している病院や検査機関に渡してもよい。The expiration gas of the alveolar air of the third phase can be collected from the expiration gas collection bag 5 into a container (not shown) which is suctioned and installed by the suction device 7 as described above. After the collection bag 5 is set to be detachable and the expiration gas of the third phase alveoli is collected there,
The expired gas collection bag 5 may be removed and delivered to a hospital or a laboratory having a mass spectrometry system.
【００１５】なお図２において、第１相は息を呼出する
前に吸い込んだ最後の空気が口腔、気管でしか吸い込ま
れず、肺にてガス交換に寄与せずに呼出により再び排出
される相で、炭酸ガスに変化は認められず、空気と同等
の濃度で推移する。第２相は、肺にてガス交換に寄与し
たガスと、口腔、気管にてガス交換に寄与していないガ
スとの混合相である。第３相は、肺においてガス交換に
寄与したガスが吐き出された相である。In FIG. 2, the first phase is a phase in which the last air inhaled before exhalation is inhaled only in the oral cavity and trachea, and is exhausted again by exhalation without contributing to gas exchange in the lungs. No change is observed in carbon dioxide gas, and the concentration changes at the same level as air. The second phase is a mixed phase of a gas that has contributed to gas exchange in the lungs and a gas that has not contributed to gas exchange in the oral cavity and trachea. The third phase is a phase in which gas that has contributed to gas exchange in the lungs is exhaled.
【００１６】[0016]
【発明の効果】本発明によれば、呼気ガス採取バッグ５
に第３相の肺胞気のみが採取されるので、正確な同位体
比が測定可能となる。また、質量分析システムを有して
いない病院や検査機関でも、呼気ガス採取バッグ５の中
に第３相の肺胞気のみが採取されるため、前記システム
を有する機関にこれを持ち込むことができる。According to the present invention, the expired gas collection bag 5
Since only the third phase of alveolar air is collected, an accurate isotope ratio can be measured. In addition, even in a hospital or a laboratory which does not have a mass spectrometry system, only the third phase alveoli is collected in the expired gas collection bag 5, so that it can be brought to an institution having the system.
【図１】本発明の一実施態様の安定同位体呼気ガス採取
装置を示す構成図である。FIG. 1 is a configuration diagram showing a stable isotope exhalation gas sampling apparatus according to an embodiment of the present invention.
【図２】測定により得られる炭酸ガス濃度変化の態様を
示す特性図である。FIG. 2 is a characteristic diagram showing an aspect of a carbon dioxide gas concentration change obtained by measurement.
１ マウスピース取り付け口 ２ 弾性バッグ ３ 圧力バルブ ４，６ チェックバルブ ５ 呼気ガス採取バッグ ７ 吸引装置 ８ 吐出バルブ DESCRIPTION OF SYMBOLS 1 Mouthpiece attachment opening 2 Elastic bag 3 Pressure valve 4, 6 Check valve 5 Exhaled gas sampling bag 7 Suction device 8 Discharge valve
Claims (4)
ガスを、マウスピースを通じて閉鎖系で密封可能な弾性
バッグに直接的に採取し、前記弾性バッグに１回換気量
の１／３〜１／２あるいはそれ以上の呼気ガスが採取さ
れた後に、前記被験者からの呼気ガスを、圧力バルブと
呼気ガスを下流方向にのみ流出することのできるチェッ
クバルブとを介して閉鎖系で密封可能な呼気ガス採取バ
ッグにさらに採取することを特徴とする安定同位体呼気
ガス採取方法。1. An exhaled gas from a subject to which a stable isotope has been administered is directly collected through a mouthpiece into an elastic bag that can be sealed in a closed system, and the elastic bag is provided with 1/3 to 1 of the tidal volume. / 2 or more of the exhaled gas is collected, after which the exhaled gas from the subject can be closed in a closed system via a pressure valve and a check valve which allows the exhaled gas to flow only downstream. A method for collecting stable isotope breath gas, further comprising collecting the gas in a gas collection bag.
ピース取り付け口１と、前記マウスピース取り付け口１
に接続された弾性バッグ２と、前記弾性バッグ２と接続
された呼気ガス採取バッグ５と、前記弾性バッグ２と前
記呼気ガス採取バッグ５との間に設けられた、前記弾性
バッグ中の圧力が設定値に到達したときに開放可能な圧
力バルブ３と、前記圧力バルブ３と前記呼気ガス採取バ
ッグ５との間に設けられた、呼気ガスを下流方向にのみ
流出することのできるチェックバルブ４と、を備えてな
る安定同位体呼気ガス採取装置。2. A mouthpiece mounting port 1 for mounting a mouthpiece, and said mouthpiece mounting port 1
The breathing gas collection bag 5 connected to the elastic bag 2, and the pressure in the elastic bag provided between the elastic bag 2 and the breathing gas collection bag 5, A pressure valve 3 that can be opened when the set value is reached, and a check valve 4 that is provided between the pressure valve 3 and the exhaled gas collection bag 5 and that allows the exhaled gas to flow only in the downstream direction. A stable isotope breath gas sampling device comprising:
気ガス採取バッグ５内のガスを吸引可能な吸引装置７が
接続され、前記呼気ガス採取バッグ５と吸引装置７との
間に、ガスを下流方向にのみ流出することのできるチェ
ックバルブ６が設けられている請求項２に記載の安定同
位体呼気ガス採取装置。3. A suction device 7 capable of sucking gas in the expiration gas collection bag 5 is connected to a stage subsequent to the expiration gas collection bag 5, and a gas is provided between the expiration gas collection bag 5 and the suction device 7. 3. The stable isotope exhalation gas collection device according to claim 2, further comprising a check valve 6 that allows the gas to flow out only in the downstream direction.
気できる吐出バルブ８がさらに設けられている請求項２
または３に記載の安定同位体呼気ガス採取装置。4. A discharge valve 8 for exhausting gas stored in the elastic bag 2 is further provided.
Or a stable isotope breath gas sampling apparatus according to item 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9245242A JPH1176203A (en) | 1997-09-10 | 1997-09-10 | Stable isotope exhalation gas collecting method, and device to be used for the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9245242A JPH1176203A (en) | 1997-09-10 | 1997-09-10 | Stable isotope exhalation gas collecting method, and device to be used for the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH1176203A true JPH1176203A (en) | 1999-03-23 |
Family
ID=17130780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9245242A Pending JPH1176203A (en) | 1997-09-10 | 1997-09-10 | Stable isotope exhalation gas collecting method, and device to be used for the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH1176203A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008102048A (en) * | 2006-10-20 | 2008-05-01 | Hitachi Ltd | Gas component collector, gas component collection apparatus, filter manufacturing method, gas component analyzer and gas component analyzing method |
-
1997
- 1997-09-10 JP JP9245242A patent/JPH1176203A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008102048A (en) * | 2006-10-20 | 2008-05-01 | Hitachi Ltd | Gas component collector, gas component collection apparatus, filter manufacturing method, gas component analyzer and gas component analyzing method |
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