JPH11511326A - アデノウィルスおよびaavの精製 - Google Patents
アデノウィルスおよびaavの精製Info
- Publication number
- JPH11511326A JPH11511326A JP9510556A JP51055697A JPH11511326A JP H11511326 A JPH11511326 A JP H11511326A JP 9510556 A JP9510556 A JP 9510556A JP 51055697 A JP51055697 A JP 51055697A JP H11511326 A JPH11511326 A JP H11511326A
- Authority
- JP
- Japan
- Prior art keywords
- adenovirus
- aav
- virus
- resin
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.活性なそして感染性のアデノウィルスを、このウィルスに対して親和性を 有する結合性基を有するクロマトグラフィマトリックス材料と接触させる工程を 含み、しかも前記結合性基は、損傷されない形で前記ウィルスを通過させるのに 十分大きい、前記マトリックス中の細孔中に閉じ込められている、活性なそして 感染性のアデノウィルスを精製する方法。 2.活性なそして感染性のアデノウィルスを、細孔を含みそして前記ウィルス に対しての親和性を有する結合性基を有するクロマトグラフィマトリックス材料 と接触させる工程を含み、しかも前記マトリックスは、該ウィルスがその前記細 孔と接触するのを実質的に防止するのに十分な架橋または触手をさらに含む、活 性なそして感染性のアデノウィルスを精製する方法。 3.アデノウィルスをも含有するサンプルからアデノ随伴ウィルスを精製する 方法において、前記サンプルを、アデノウィルスを損傷することが出来るクロマ トグラフィ材料と接触させる工程を含み、しかも前記損傷されたアデノウィルス は非感染性となる、前記方法。 4.本明細書中の開示と実質的に一致するアデノ随伴ウィルスを精製する方法 。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US296795P | 1995-08-30 | 1995-08-30 | |
US60/002,967 | 1995-08-30 | ||
PCT/US1996/013872 WO1997008298A1 (en) | 1995-08-30 | 1996-08-30 | Chromatographic purification of adenovirus and aav |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006289363A Division JP2007082552A (ja) | 1995-08-30 | 2006-10-25 | アデノウィルスおよびaavの精製 |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH11511326A true JPH11511326A (ja) | 1999-10-05 |
Family
ID=21703422
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9510556A Withdrawn JPH11511326A (ja) | 1995-08-30 | 1996-08-30 | アデノウィルスおよびaavの精製 |
JP2006289363A Withdrawn JP2007082552A (ja) | 1995-08-30 | 2006-10-25 | アデノウィルスおよびaavの精製 |
JP2007262817A Expired - Lifetime JP4612034B2 (ja) | 1995-08-30 | 2007-10-09 | アデノウィルスおよびaavの精製 |
JP2010120528A Pending JP2010207244A (ja) | 1995-08-30 | 2010-05-26 | アデノウィルスおよびaavの精製 |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006289363A Withdrawn JP2007082552A (ja) | 1995-08-30 | 2006-10-25 | アデノウィルスおよびaavの精製 |
JP2007262817A Expired - Lifetime JP4612034B2 (ja) | 1995-08-30 | 2007-10-09 | アデノウィルスおよびaavの精製 |
JP2010120528A Pending JP2010207244A (ja) | 1995-08-30 | 2010-05-26 | アデノウィルスおよびaavの精製 |
Country Status (6)
Country | Link |
---|---|
US (4) | US6143548A (ja) |
EP (1) | EP0847442A1 (ja) |
JP (4) | JPH11511326A (ja) |
AU (1) | AU722196B2 (ja) |
CA (2) | CA2230655C (ja) |
WO (1) | WO1997008298A1 (ja) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003505033A (ja) * | 1999-07-19 | 2003-02-12 | メディジーン・アクチェンゲゼルシャフト | 修飾されたクロマトグラフィー特性を有するアデノ随伴ウイルスの構造タンパク質と、その製造及び使用 |
JP2010155868A (ja) * | 2003-05-21 | 2010-07-15 | Genzyme Corp | 空キャプシドを実質的に含まない組換えaavビリオン調製物を生成するための方法 |
WO2012144446A1 (ja) | 2011-04-18 | 2012-10-26 | 独立行政法人国立精神・神経医療研究センター | 薬剤送達粒子及びその製造方法 |
JP2012529917A (ja) * | 2009-06-16 | 2012-11-29 | ジェンザイム・コーポレーション | 組換えaavベクターの改良された精製方法 |
WO2021002257A1 (ja) * | 2019-07-04 | 2021-01-07 | 株式会社カネカ | ウイルスまたはウイルス様粒子の精製方法 |
JP2022526639A (ja) * | 2019-04-11 | 2022-05-25 | リジェネクスバイオ インコーポレイテッド | 組換えアデノ随伴ウイルス組成物の特性評価のためのサイズ排除クロマトグラフィーの方法 |
Families Citing this family (255)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH11511326A (ja) * | 1995-08-30 | 1999-10-05 | ジエンザイム コーポレイション | アデノウィルスおよびaavの精製 |
EP1707631A3 (en) * | 1996-11-20 | 2006-12-27 | Introgen Therapeutics, Inc. | An improved method for the production and purification of adenoviral vectors |
US7732129B1 (en) | 1998-12-01 | 2010-06-08 | Crucell Holland B.V. | Method for the production and purification of adenoviral vectors |
AU5370598A (en) | 1996-12-13 | 1998-07-03 | Schering Corporation | Methods for purifying viruses |
DE19709186C2 (de) * | 1997-03-06 | 1999-10-14 | Medigene Ag | Filtrationsverfahren zur Trennung von Viren |
US6020191A (en) * | 1997-04-14 | 2000-02-01 | Genzyme Corporation | Adenoviral vectors capable of facilitating increased persistence of transgene expression |
US5981275A (en) * | 1997-04-14 | 1999-11-09 | Genzyme Corporation | Transgene expression system for increased persistence |
SE9702405D0 (sv) * | 1997-06-24 | 1997-06-24 | Pharmacia Biotech Ab | Chromatography materials, a process for their preparation and use of the materials |
DE19738292C1 (de) * | 1997-09-02 | 1999-05-12 | Deutsches Krebsforsch | Verfahren zur Reinigung und Konzentrierung von AAV-2 sowie antigenen Teilen davon |
US6989264B2 (en) * | 1997-09-05 | 2006-01-24 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
US6566118B1 (en) | 1997-09-05 | 2003-05-20 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
ES2557997T3 (es) * | 1997-09-05 | 2016-02-01 | Genzyme Corporation | Métodos para generar preparados de vectores AAV de título elevado sin virus auxiliar |
US6410300B1 (en) | 1998-01-12 | 2002-06-25 | The University Of North Carolina At Chapel Hill | Methods and formulations for mediating adeno-associated virus (AAV) attachment and infection and methods for purifying AAV |
US6984635B1 (en) | 1998-02-13 | 2006-01-10 | Board Of Trustees Of The Leland Stanford Jr. University | Dimerizing agents, their production and use |
EP1073721B1 (en) * | 1998-04-22 | 2005-08-31 | Genvec, Inc. | Efficient purification of adenovirus |
US6146874A (en) * | 1998-05-27 | 2000-11-14 | University Of Florida | Method of preparing recombinant adeno-associated virus compositions |
ATE324436T1 (de) | 1998-08-14 | 2006-05-15 | Merck & Co Inc | Verfahren zur reinigung von menschlichen, dem papillomavirus ähnlichen partikeln. |
EP1930418B1 (en) | 1998-09-04 | 2015-04-08 | Genzyme Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
JP4472182B2 (ja) * | 1998-09-04 | 2010-06-02 | ターゲティッド ジェネティクス コーポレイション | 放出された組換えaavベクターの高力価のヘルパーを含まない調製物を生成するための方法 |
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1996
- 1996-08-30 JP JP9510556A patent/JPH11511326A/ja not_active Withdrawn
- 1996-08-30 EP EP96931425A patent/EP0847442A1/en not_active Withdrawn
- 1996-08-30 CA CA002230655A patent/CA2230655C/en not_active Expired - Lifetime
- 1996-08-30 US US09/011,828 patent/US6143548A/en not_active Expired - Lifetime
- 1996-08-30 WO PCT/US1996/013872 patent/WO1997008298A1/en not_active Application Discontinuation
- 1996-08-30 CA CA002625279A patent/CA2625279A1/en not_active Abandoned
- 1996-08-30 AU AU70108/96A patent/AU722196B2/en not_active Ceased
-
2004
- 2004-03-02 US US10/470,604 patent/US7015026B2/en not_active Expired - Fee Related
-
2005
- 2005-07-12 US US11/179,865 patent/US7579181B2/en not_active Expired - Fee Related
-
2006
- 2006-10-25 JP JP2006289363A patent/JP2007082552A/ja not_active Withdrawn
-
2007
- 2007-10-09 JP JP2007262817A patent/JP4612034B2/ja not_active Expired - Lifetime
-
2009
- 2009-07-16 US US12/504,073 patent/US20100279385A1/en not_active Abandoned
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2010
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JP2003505033A (ja) * | 1999-07-19 | 2003-02-12 | メディジーン・アクチェンゲゼルシャフト | 修飾されたクロマトグラフィー特性を有するアデノ随伴ウイルスの構造タンパク質と、その製造及び使用 |
JP2010155868A (ja) * | 2003-05-21 | 2010-07-15 | Genzyme Corp | 空キャプシドを実質的に含まない組換えaavビリオン調製物を生成するための方法 |
JP2012529917A (ja) * | 2009-06-16 | 2012-11-29 | ジェンザイム・コーポレーション | 組換えaavベクターの改良された精製方法 |
US10017746B2 (en) | 2009-06-16 | 2018-07-10 | Genzyme Corporation | Methods for purification of recombinant AAV vectors |
JP2019083822A (ja) * | 2009-06-16 | 2019-06-06 | ジェンザイム・コーポレーション | 組換えaavベクターの改良された精製方法 |
US10696952B2 (en) | 2009-06-16 | 2020-06-30 | Genzyme Corporation | Methods for purification of recombinant AAV vectors |
US11840710B2 (en) | 2009-06-16 | 2023-12-12 | Genzyme Corporation | Methods for purification of recombinant AAV vectors |
WO2012144446A1 (ja) | 2011-04-18 | 2012-10-26 | 独立行政法人国立精神・神経医療研究センター | 薬剤送達粒子及びその製造方法 |
EP3219313A2 (en) | 2011-04-18 | 2017-09-20 | National Center of Neurology and Psychiatry | Drug delivery particle and method for producing the same |
EP3777843A1 (en) | 2011-04-18 | 2021-02-17 | National Center of Neurology and Psychiatry | Drug delivery particle and method for producing the same |
JP2022526639A (ja) * | 2019-04-11 | 2022-05-25 | リジェネクスバイオ インコーポレイテッド | 組換えアデノ随伴ウイルス組成物の特性評価のためのサイズ排除クロマトグラフィーの方法 |
WO2021002257A1 (ja) * | 2019-07-04 | 2021-01-07 | 株式会社カネカ | ウイルスまたはウイルス様粒子の精製方法 |
Also Published As
Publication number | Publication date |
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US20040152183A1 (en) | 2004-08-05 |
JP4612034B2 (ja) | 2011-01-12 |
JP2007082552A (ja) | 2007-04-05 |
US7015026B2 (en) | 2006-03-21 |
WO1997008298A1 (en) | 1997-03-06 |
US20050244942A1 (en) | 2005-11-03 |
CA2625279A1 (en) | 1997-03-06 |
AU722196B2 (en) | 2000-07-27 |
CA2230655A1 (en) | 1997-03-06 |
US6143548A (en) | 2000-11-07 |
JP2008092951A (ja) | 2008-04-24 |
EP0847442A1 (en) | 1998-06-17 |
US7579181B2 (en) | 2009-08-25 |
AU7010896A (en) | 1997-03-19 |
CA2230655C (en) | 2008-06-17 |
JP2010207244A (ja) | 2010-09-24 |
US20100279385A1 (en) | 2010-11-04 |
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