JPH11263795A - Active oxygen deletion ability deterioration inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject active oxygen deletion ability deterioration inhibitor that can markedly inhibit the deterioration of the ability to delete active oxygen originating from plants by using trehalose as an active ingredient and is useful for maintenance and increase of health. SOLUTION: This inhibitory agent comprises trehalose as an active ingredient, preferably in an amount of about >=20 w/w % calculated as the trehalose anhydride, additional saccharicies other than trehalose, pullulan and/or cyclodextrin. In a preferred embodiment, the substance having the active oxygen deletion activity, originating from plants is combined with trehalose or the objective inhibitory agent is admixed in an aqueous medium, for example, trehalose is homogeneously mixed and dissolved in the edible plant substance and/or an antioxidant plant substance in an aqueous medium as long as possible.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【０００１】[0001]

【発明の属する技術分野】本発明は、活性酸素消去能低
減抑制剤とその用途に関し、詳細には、トレハロースを
有効成分とする活性酸素消去能低減抑制剤と、トレハロ
ース又は該低減抑制剤を水性媒体で含有せしめることを
特徴とする植物由来の活性酸素消去能の低減抑制方法、
並びに該方法により活性酸素消去能の低減を抑制した植
物性可食物及び／又は植物性抗酸化物質含有組成物に関
する。The present invention relates to an active oxygen scavenging ability reduction inhibitor and its use. More specifically, the present invention relates to an active oxygen scavenging ability reduction inhibitor containing trehalose as an active ingredient, and an aqueous solution of trehalose or the reduction inhibitor. A method for suppressing reduction of the ability to scavenge active oxygen derived from plants, characterized by being contained in a medium,
Further, the present invention relates to a plant edible food and / or a plant antioxidant-containing composition in which the reduction of active oxygen scavenging ability is suppressed by the method.

【０００２】[0002]

【従来の技術】野菜、きのこ、海藻等の可食性植物は、
生体にとって、ビタミンはもとより、ミネラル、食物繊
維等の機能性成分の給源として重要であり、発育、成長
に欠くべからざる食材であることはよく知られている。
これに加えて、近年、これら可食性植物には、健康の維
持増進、老化防止、成人病の予防、発癌抑制等に深く関
係している活性酸素消去能の存在が注目されるようにな
ってきた。生体の老化及びこの老化に伴う各種疾患、例
えば、癌、動脈硬化、肝硬変、心筋梗塞、脳卒中、白内
障、パーキンソン病、リューマチ、アルツハイマー性痴
呆等、いわゆる難病といわれる疾患の発生機構に関して
は未だ不明な点が多い。しかしながら、近年、これら難
病の発生機構として、不対電子を持つ酸素で反応性に富
んだスーパーオキシド、これから生じるヒドロキシラジ
カル、過酸化水素等の活性酸素が、細胞内の標的分子、
例えば、膜脂質、蛋白質、ＤＮＡ等を酸化し、酸素傷害
を引き起こし、結果として、生体の老化や老化に伴う各
種疾患を招くと考えられるようになってきた。BACKGROUND ART Edible plants such as vegetables, mushrooms and seaweed are
It is well known that living organisms are important as a source of functional components such as minerals and dietary fiber, as well as vitamins, and are indispensable ingredients for growth and growth.
In addition, in recent years, these edible plants have attracted attention for their ability to scavenge active oxygen, which is closely related to maintaining and improving health, preventing aging, preventing adult diseases, and suppressing carcinogenesis. Was. Aging of the living body and various diseases associated with this aging, for example, cancer, arteriosclerosis, liver cirrhosis, myocardial infarction, stroke, cataract, Parkinson's disease, rheumatism, Alzheimer's dementia, etc. There are many points. However, in recent years, as an inducing mechanism of these intractable diseases, active oxygen such as superoxide, which is rich in reactivity with oxygen having unpaired electrons, and a resulting hydroxy radical and hydrogen peroxide, is a target molecule in cells,
For example, it has been considered that membrane lipids, proteins, DNA, and the like are oxidized to cause oxygen damage, resulting in aging of living bodies and various diseases associated with aging.

【０００３】細胞内には、本来、活性酸素を消去する酵
素、例えば、スーパーオキシド・ジスムターゼ（ＥＣ
１．１５．１．１）、カタラーゼ（ＥＣ １．１１．
１．６）等の酵素や、Ｌ−アスコルビン酸、α−トロフ
ェロール等の抗酸化物質が存在していて、その活性酸素
の濃度は、通常、きわめて低い状態に保たれている。し
かしながら、大量の紫外線、放射線、電磁波等をあびた
り、過激な運動をしたり、精神的に強烈なストレスを受
けたり、これらに加えて、加齢が進んだりすると、活性
酸素の生成量に活性酸素消去能が追いつかず、結果とし
て過剰な活性酸素により酸化された化合物が蓄積するこ
ととなり、前記の酸素傷害を引き起こすものと考えられ
ている。これを改善するために、例えば、特開平５−１
６８４３５号公報、特開平８−１４３４６６号公報等に
開示されているように、可食性植物に含まれる高い活性
酸素消去能を利用して、生体に不足しがちな活性酸素消
去能を補って、健康の維持増進を計ろうとする提案がな
されている。しかしながら、これら活性酸素消去能の比
較的高い可食性植物を使用する場合であっても、これに
搾汁、抽出、加熱、乾燥等の加工処理を施したり、その
後の保存条件によっては、それが持つ本来の活性酸素消
去能を著しく低減させるか、ひどい場合には、全く無く
してしまう欠点のあることが判明した。この欠点を解消
するために、活性酸素消去能の低減を抑制する全く新し
い方法の確立が強く望まれる。しかも、生体にとって、
毒性を懸念することのない方法の確立が望まれる。[0003] In cells, enzymes that naturally eliminate active oxygen, for example, superoxide dismutase (EC
1.15.1.1), catalase (EC 1.11.
1.6) and antioxidants such as L-ascorbic acid and α-tropherol, and the concentration of active oxygen is usually kept extremely low. However, when exposed to a large amount of ultraviolet rays, radiation, electromagnetic waves, etc., exercises intensely, receive mentally intense stress, and in addition to these, as aging progresses, it increases the amount of active oxygen generated It is thought that the oxygen scavenging ability cannot keep up, and as a result, compounds oxidized by excessive active oxygen accumulate, causing the above-mentioned oxygen damage. In order to improve this, for example, Japanese Patent Application Laid-Open
No. 68435, as disclosed in JP-A-8-143466, etc., utilizing the high active oxygen scavenging ability contained in edible plants, supplementing the active oxygen scavenging ability that is apt to be lacking in living organisms, There have been proposals to maintain and improve health. However, even when using these edible plants having relatively high active oxygen scavenging ability, they may be subjected to processing such as squeezing, extraction, heating, drying, etc. It has been found that there is a disadvantage that the original active oxygen scavenging ability possessed is significantly reduced or, in severe cases, completely eliminated. In order to solve this drawback, it is strongly desired to establish a completely new method for suppressing the reduction of the active oxygen scavenging ability. And for the living body,
It is desirable to establish a method that does not raise concerns about toxicity.

【０００４】[0004]

【発明が解決しようとする課題】本発明は、新規な活性
酸素消去能低減抑制剤と新規な活性酸素消去能の低減抑
制方法を確立するとともに、該方法を採用して活性酸素
消去能の低減を抑制した植物性可食物及び／又は植物性
抗酸化物質含有組成物を提供することを課題とするもの
である。SUMMARY OF THE INVENTION The present invention establishes a novel active oxygen scavenging ability reduction inhibitor and a novel active oxygen scavenging ability reduction suppressing method, and employs the method to reduce the active oxygen scavenging ability. It is an object of the present invention to provide a plant edible food and / or a plant antioxidant-containing composition in which the composition is suppressed.

【０００５】[0005]

【課題を解決するための手段】本発明者等は、上記課題
を解決するために、糖質の利用に着目し、鋭意研究を続
けてきた。即ち、植物性可食物や植物性抗酸化物質等の
植物由来の活性酸素消去能を有する物質に各種糖質を含
有させ、その活性酸素消去能の低減抑制効果に与える糖
質の影響を調べた。その結果、トレハロースが、他の糖
質に比較して著効を示し、植物由来の活性酸素消去能の
低減を著しく抑制することを見いだし、本発明を完成し
た。Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have focused on the use of carbohydrates and have continued intensive research. That is, various carbohydrates were added to a plant-derived substance having active oxygen scavenging ability, such as plant edible foods and plant antioxidants, and the effect of carbohydrates on the effect of suppressing the reduction of the active oxygen scavenging ability was investigated. . As a result, they have found that trehalose is significantly more effective than other carbohydrates and significantly suppresses the reduction of the ability to eliminate plant-derived active oxygen, thus completing the present invention.

【０００６】[0006]

【発明の実施の形態】本発明の主な目的は、第一とし
て、トレハロースを有効成分とする活性酸素消去能の低
減抑制剤を提供することであり、第二として、植物由来
の活性酸素消去能を有する物質にトレハロース又は該低
減抑制剤を含有せしめることを特徴とする植物由来の活
性酸素消去能低減抑制方法を提供することであり、第三
として、該方法により活性酸素消去能の低減を抑制した
組成物を提供することである。BEST MODE FOR CARRYING OUT THE INVENTION The main object of the present invention is to firstly provide a suppressive agent for reducing active oxygen scavenging ability comprising trehalose as an active ingredient, and secondly, to eliminate plant-derived active oxygen. The present invention is to provide a method for inhibiting the reduction of active oxygen scavenging ability derived from plants, characterized by including trehalose or the reduction inhibitor in a substance having an ability. The purpose is to provide a composition with reduced content.

【０００７】本発明で使用するトレハロースは、α，α
−トレハロースが好ましく、活性酸素消去能の低減を抑
制できるものであれば、その由来、性状を問わない。ト
レハロースの由来としては、例えば、特開平７−２４６
０９７号公報に記載される酵母からのトレハロース、特
開昭５８−２１６６９５号公報、特開平７−１７０９７
７号公報等に記載されるマルトースからのトレハロー
ス、更には、特開平７−１４３８７６号公報、特開平７
−２１３２８３号公報等に記載される澱粉からのトレハ
ロース等各種トレハロース含有糖質が適宜採用できる。
また、その性状としては、シラップ、含蜜結晶粉末、含
水結晶、無水結晶、非晶質固体等適宜採用できる。勿
論、市販の高純度トレハロース含水結晶、高純度トレハ
ロース無水結晶等を使用することも有利に実施できる。
使用される糖質のトレハロースの含量は、植物性可食物
等に含有せしめて、植物性可食物等が持つ活性酸素消去
能の低減の抑制効果を発揮できればよく、通常、無水物
換算で、約１０ｗ／ｗ％（以下、本明細書では、特に断
らない限り、ｗ／ｗ％を単に％で示す。）以上、望まし
くは、約２０％以上、更に望ましくは約５０％以上が好
適である。また、本発明の植物性可食物及び／又は植物
性抗酸化物質含有組成物に対して、トレハロースとし
て、無水物換算で、通常約１％以上、望ましくは、約５
％以上、更に好ましくは約２０％以上含有せしめるのが
好適である。一般に、トレハロースが、無水物換算で約
１％未満では活性酸素消去能の低減抑制作用が不充分
で、約３０％以上では得られる組成物に甘味が付くきら
いがある。The trehalose used in the present invention is α, α
-Trehalose is preferred, regardless of its origin and properties as long as it can suppress the reduction of the active oxygen scavenging ability. The origin of trehalose is described in, for example, JP-A-7-246.
No. 097, Trehalose from yeast, JP-A-58-21669, JP-A-7-17097
Trehalose from maltose described in Japanese Patent Application Laid-Open No. 7-143876 and Japanese Patent Application Laid-Open No.
Various trehalose-containing saccharides such as trehalose from starch described in JP-A-213283 and the like can be appropriately employed.
In addition, as its properties, syrup, honey-containing crystal powder, hydrated crystal, anhydrous crystal, amorphous solid and the like can be appropriately adopted. Of course, it is also advantageous to use commercially available high-purity trehalose hydrous crystals, high-purity trehalose anhydrous crystals, or the like.
The content of trehalose in the carbohydrate used is not limited as long as it can be contained in a vegetable edible food or the like so as to exert the effect of suppressing the reduction of the active oxygen scavenging ability of the vegetable edible food or the like. It is preferably at least 10 w / w% (hereinafter, w / w% is simply indicated by% unless otherwise specified), preferably at least about 20%, more preferably at least about 50%. In addition, the edible food and / or the plant antioxidant-containing composition of the present invention, as trehalose, is usually about 1% or more, preferably about 5%, in terms of anhydride, as trehalose.
%, More preferably about 20% or more. In general, if trehalose is less than about 1% in terms of anhydride, the effect of suppressing the reduction of the active oxygen scavenging ability is insufficient, and if it is about 30% or more, the resulting composition tends to be sweet.

【０００８】しかし、本発明の組成物に甘味が付いても
かまわない場合、又はむしろ甘味が付く方が好ましい場
合、例えば、珍味、スナック食品、キャンデー風食品等
を製造する場合には、可食性植物の組織、例えば、可食
性植物の器官、組織、それら可食物の切断物及び漬物等
から選ばれる植物組織の内部に、それに含まれる水分と
部分的に置換して、トレハロースを無水物換算で約３０
％以上、望ましくは約４０％以上、更に望ましくは５０
％以上を水性媒体で含有せしめた組成物を得ることも有
利に実施できる。この際、該植物組織の内部に存在する
トレハロースは、非晶質状態で含有していても、又、含
水結晶状態で含有していてもよく、いずれの状態の場合
も有利に実施できる。更に、トレハロースを水性媒体で
含有せしめる工程と共に、殺菌及び／又は乾燥する工程
を施すことも有利に実施できる。このようにして得られ
る固状組成物は、活性酸素消去能の低減抑制に優れてい
るのみならず、吸湿性も低く、室内環境で安定で、取扱
い容易である。また、この固状組成物は、トレハロース
により植物組織がその形状をよく維持してしっかりして
おり、歯ごたえのある感触を楽しむことのできる固状食
品として好適である。However, when the composition of the present invention may be sweetened, or more preferably sweetened, for example, when producing a delicacy, snack food, candy-like food, etc., the composition may be edible. Plant tissue, for example, edible plant organs, tissues, inside of plant tissue selected from such edible cuts and pickles, partially replace the water contained therein, trehalose in anhydrous equivalent About 30
% Or more, preferably about 40% or more, more preferably 50% or more.
% Can be advantageously implemented. At this time, trehalose present in the plant tissue may be contained in an amorphous state or in a hydrated crystalline state, and it can be advantageously carried out in any state. Further, it is possible to advantageously carry out a sterilization and / or drying step together with the step of containing trehalose in an aqueous medium. The solid composition thus obtained is not only excellent in suppressing the reduction of the active oxygen scavenging ability, but also has low hygroscopicity, is stable in an indoor environment, and is easy to handle. In addition, the solid composition is suitable as a solid food in which the plant tissue maintains its shape well and is firm by trehalose, and can enjoy a chewy feel.

【０００９】本発明でいう含有せしめるとは、共存させ
ることをいい、植物由来の活性酸素消去能を有する物質
に本発明のトレハロースを含有せしめる場合には、それ
によって、植物由来の活性酸素消去能の低減を抑制させ
る効果が発揮できればよく、含有せしめる方法や両者の
相対的な割合は特に問うものではない。望ましくは、植
物性可食物及び／又は植物性抗酸化物質に対して、トレ
ハロースを水性媒体でできるだけ均一に接触させて含有
せしめるのがよい。具体的に述べれば、例えば、植物性
可食物等が、液状乃至懸濁状物のような多汁状態である
場合には、これに粉末、結晶等の固状のトレハロースを
できるだけ均一に混合溶解させて含有せしめ、又はシラ
ップ状のトレハロースをできるだけ均一に混合して含有
せしめればよい。また、植物性可食物等が固状である場
合には、これを水で液状乃至懸濁状物のような多汁状態
にした後、前述のように処理して含有せしめるか、又
は、シラップ状のトレハロースを用意し、これに固状の
植物性可食物等を分散、溶解乃至懸濁し、できるだけ均
一に接触させて、植物性可食物及び／又は植物性抗酸化
物質にトレハロースを含有せしめればよい。また、植物
性可食物が、生の植物組織を細断、千切り又はスライス
等切断したもののような場合には、これに固状トレハロ
ースをふりかけ、混合して溶解含有させるか又はシラッ
プ状トレハロース溶液に浸漬する等して、該植物の組織
内部にできるだけ均一にトレハロースを含有せしめれば
よい。植物性可食物等にトレハロースを接触含有せしめ
る際の温度、時間等の条件は、必要に応じて適宜選択で
きる。In the present invention, the term "contain" means to coexist. When the trehalose of the present invention is contained in a substance having a plant-derived active oxygen-eliminating ability, the plant-derived active oxygen-eliminating ability is thereby reduced. It is sufficient that the effect of suppressing the reduction can be exerted, and there is no particular limitation on the method of incorporation and the relative ratio between the two. Desirably, trehalose is contained in the vegetable edible food and / or the plant antioxidant by bringing the trehalose into contact with the aqueous medium as uniformly as possible. More specifically, for example, when the vegetable edible food or the like is in a multi-juice state such as a liquid or a suspension, a solid trehalose such as a powder or a crystal is mixed and dissolved as uniformly as possible. The syrup-shaped trehalose may be mixed and contained as uniformly as possible. Further, when the vegetable edible food or the like is in a solid state, it is converted into a liquid or a suspension like water in a multiple juice state with water and then treated as described above for inclusion, or syrup. Trehalose in the form of a solid, and disperse, dissolve or suspend a solid plant edible substance or the like into the trehalose, and make the edible substance and / or the plant antioxidant contain trehalose by contacting as uniformly as possible. I just need. Also, when the plant edible food is such as a raw plant tissue cut, shredded or sliced, sprinkle with solid trehalose and mix to dissolve or contain the trehalose solution. Trehalose may be contained as uniformly as possible in the tissue of the plant by immersion or the like. Conditions such as temperature and time for bringing trehalose into contact with a plant edible food or the like can be appropriately selected as necessary.

【００１０】本発明でいう植物由来の活性酸素消去能を
有する物質とは、可食性植物の器官及び／又は組織その
ものや、可食性植物のそれら可食部の切断物、破砕物、
粉砕物、乾燥物、漬物、該可食部の抽出物及び／又は植
物性抗酸化物質等を意味する。可食性植物としては、例
えば、人参、れんこん、玉ねぎ、ごぼう、大根、里芋、
やまいも、さつまいも、じゃがいも等の根菜、レタス、
チコリ、白菜、キャベツ、ケール、モロヘイヤ、あした
ば、ほうれん草、つるむらさき、小松菜、野沢菜、春
菊、チンゲンサイ、かぶ等葉菜、オクラ、カリフラワ
ー、ブロッコリー、なす、トマト、きゅうり、かぼち
ゃ、ズッキーニ、ピーマン、さやえんどう、さやいんげ
ん等果菜、アルファルファ、大豆もやし、緑豆もやし等
もやし類等の野菜類、椎茸、えのき、しめじ等の茸類、
ひじき、わかめ、こんぶ等の海草類、レモン、柚、すだ
ち、ざぼん、きんかん等柑橘、バナナ、パイナップル、
キーウィフルーツ、いちご、サンザシ、ブルーベリー、
ぶどう、もも、りんご、なし、くり等の果物類、にんに
く、生姜、わさび、からし、パセリ、中国パセリ、青じ
そ、赤じそ、にら、ねぎ、セロリ、せり、クレソン、と
うがらし、さんしょう、ペパー、ローズマリー、はっか
等のハーブ類、よもぎ、おおばこ、どくだみ、毒消し、
せんぶり、アロエ、甘草、うこん、藍、ブラジル人参
（パフィア）、ビワの葉、杉菜、松葉、笹葉、梅実、
茶、大麦若葉、そば葉、イチョウ葉、杜仲茶、バナバ、
ルイボス、ギムネマ等の薬草、生薬類、その他、はとむ
ぎ、そば、ごま、米、麦、とうもろこし、空豆、大豆、
落花生、クルミ、松の実、種実胚芽等の種実類等の可食
部が適宜用いられる。また、可食性植物の漬物として
は、前述の根菜、葉菜、果菜等の野菜類の塩漬、糠漬、
麹漬、粕漬、たまり漬、酢漬、キムチ等各種の漬物が適
宜用いられる。The substance having a plant-derived ability to scavenge active oxygen referred to in the present invention includes organs and / or tissues of edible plants, cut and crushed edible parts of edible plants,
Pulverized product, dried product, pickle, extract of the edible portion and / or plant antioxidant. As edible plants, for example, carrot, lotus root, onion, burdock, radish, taro,
Root vegetables such as potatoes, sweet potatoes, potatoes, lettuce,
Chicory, Chinese cabbage, cabbage, kale, moroheiya, tofu, spinach, vine murasaki, komatsuna, nozawana, spring chrysanthemum, bok choy, turnip, etc. Fruit vegetables such as pods, alfalfa, soy bean sprouts, vegetables such as mung bean sprouts and sprouts, mushrooms such as shiitake mushrooms, enoki, shimeji,
Seaweeds such as hijiki, wakame, konbu, citrus, banana, pineapple, etc., such as lemon, yuzu, sudachi, zabon, kumquat,
Kiwifruit, strawberries, hawthorn, blueberries,
Fruits such as grapes, thighs, apples, pears, garlic, garlic, ginger, wasabi, mustard, parsley, Chinese parsley, green soybean, red soybean, leek, green onion, celery, auctioneer, watercress, red pepper, pepper, pepper , Rosemary, seaweed and other herbs, wormwood, stomach, dokudami, poison,
Senburi, aloe, licorice, konko, indigo, Brazilian ginseng (pafia), loquat leaves, cedar, matsuba, bamboo leaves, plum,
Tea, young barley leaves, buckwheat leaves, ginkgo leaves, Tochu tea, banaba,
Herbs such as rooibos and gymnema, herbal medicines, and others, such as wheat, buckwheat, sesame, rice, wheat, corn, sky beans, soybeans,
Edible parts such as peanuts, walnuts, pine nuts, seed germs such as seed germ are appropriately used. In addition, as pickles of edible plants, salting, bran pickling of vegetables such as root vegetables, leaf vegetables, and fruit vegetables described above,
Various pickles such as koji-zuke, kasu-zuke, tamari-zuke, vinegar-zuke and kimchi are appropriately used.

【００１１】植物性抗酸化物質としては、例えば、スー
パーオキシド・ジスムターゼ、カタラーゼ、パーオキシ
ダーゼ等活性酸素消去能を有する植物由来の酵素等の
他、ルチン、α−グルコシルルチン、ヘスペリジン、α
−グルコシルヘスペリジン、ナリンジン、α−グルコシ
ルナリンジン、クロロフィル、カロチン、アントシアニ
ン等の植物由来の色素、没食子酸、カテキン、α−グル
コシルカテキン、タンニン酸等植物由来のポリフェノー
ル、α−トコフェロール、Ｌ−アスコルビン酸、リボフ
ラビン等植物由来のビタミン等の植物由来の低分子抗酸
化物質の１種又は２種以上が適宜選ばれ、これらは、可
食性植物に元々含まれていてもよいし、必要に応じて、
可食性植物の可食部を加工処理する際に新たに加えられ
たものであってもよく、更に、可食性植物とは、別に、
単独で存在するものであってもよい。また、これら植物
性抗酸化物質は、必ずしも実際に植物から抽出されたも
のである必要はなく、人為的に合成、製造されたもので
あってもよい。The plant antioxidants include, for example, enzymes derived from plants having an ability to scavenge active oxygen such as superoxide dismutase, catalase and peroxidase, as well as rutin, α-glucosylrutin, hesperidin, α
-Glucosyl hesperidin, naringin, α-glucosyl naringin, chlorophyll, carotene, plant-derived pigments such as anthocyanin, gallic acid, catechin, α-glucosylcatechin, tannic acid-derived plant-derived polyphenols, α-tocopherol, L-ascorbic acid, One or more kinds of plant-derived low molecular antioxidants such as vitamins derived from plants such as riboflavin are appropriately selected, and these may be originally contained in edible plants or, if necessary,
It may be newly added at the time of processing the edible portion of the edible plant, and further, apart from the edible plant,
It may exist alone. Further, these plant antioxidants do not necessarily have to be actually extracted from plants, but may be artificially synthesized and produced.

【００１２】本発明の活性酸素消去能の低減を抑制した
組成物の製造方法について、より具体的に述べれば、例
えば、可食性植物の可食部を、必要に応じて加熱殺菌し
た後、ミキサー、ジューサー、細断機、切断機、スライ
サー等で破砕又は切断してペースト状乃至懸濁状物又は
切断物とし、これにトレハロースを混合溶解させて含有
せしめるか、又は、可食性植物の可食部を熱水、アルコ
ール等の溶媒で抽出し、濾過し、必要に応じて、濃縮し
て液状乃至ペースト状物とし、これにトレハロースを混
合溶解させて含有せしめればよい。このようにして得ら
れた組成物は、通常、シラップ状乃至ペースト状組成物
又は切断物含有多汁組成物であって、水分含量が高く、
安定に維持するのに低温貯蔵を必要とする難点がある。
これらシラップ状乃至ペースト状組成物又は切断物含有
多汁組成物を、そのまま乾燥するか若しくは乾燥、粉末
化するか、又は加熱殺菌の後、乾燥するか若しくは乾
燥、粉末化する等して、粉末状又は細片状の固状組成物
を得る。乾燥、粉末化の方法は公知の方法が適宜採用で
きる。例えば、真空乾燥、通風乾燥、ドラム乾燥の後、
粉砕、篩別するか、噴霧乾燥、流動乾燥の後、篩別する
か、更には、無水マルトース、無水トレハロース等の無
水糖質を混合し、脱水、乾燥させた後、篩別する等して
粉末製品とし、これを、必要に応じて、更に、顆粒、錠
剤、カプセル等適宜な形状にして利用することも有利に
実施できる。このような固状組成物まで加工度を高める
ことによって、活性酸素消去能低減抑制効果が更に高ま
るだけでなく、保存安定性も向上し、取り扱いも容易と
なる。また、このようにして得た固状の植物性可食物含
有組成物は、トレハロースを非晶質状態で、又は含水結
晶状態で含有するとともに、原料の可食性植物が本来持
っている、例えば、クロロフィル、カロチン、アントシ
アニン、フラボノイド等の色素の劣化を抑制し、本来の
色調をよく保持する特徴がある。また、苦味、渋味等の
嫌味をマスクする特徴もある。More specifically, the method for producing a composition of the present invention in which a reduction in the ability to scavenge active oxygen is suppressed is as follows. For example, an edible portion of an edible plant is heat-sterilized, if necessary, and then mixed. , Crushed or cut with a juicer, shredder, cutting machine, slicer, etc. to make a paste or suspension or cut, into which trehalose is mixed and dissolved and contained, or edible plant edible The part may be extracted with a solvent such as hot water or alcohol, filtered, and, if necessary, concentrated to a liquid or paste-like substance, and trehalose may be mixed and dissolved therein to contain it. The composition thus obtained is usually a syrup-like or paste-like composition or a cut-containing multi-juice composition, having a high water content,
The disadvantage is that cold storage is required to maintain stability.
These syrup-to-paste compositions or cut material-containing multi-juice compositions are dried or dried, powdered as they are, or after heat sterilization, dried or dried, powdered, etc. A solid composition in the form of a strip or a strip is obtained. Known methods can be appropriately used for the drying and powdering methods. For example, after vacuum drying, ventilation drying, drum drying,
After crushing, sieving, spray drying, fluid drying, sieving, or further mixing anhydrous saccharides such as anhydrous maltose and anhydrous trehalose, dehydrating and drying, and then sieving. It can be advantageously used in the form of a powder product, which may be used in an appropriate form such as granules, tablets, capsules, etc., if necessary. By increasing the degree of processing to such a solid composition, not only the effect of suppressing the reduction of the active oxygen scavenging ability is further enhanced, but also the storage stability is improved and the handling becomes easy. In addition, the solid plant edible food-containing composition thus obtained contains trehalose in an amorphous state or in a hydrous crystalline state, and the edible plant as a raw material originally has, for example, It is characterized by suppressing deterioration of pigments such as chlorophyll, carotene, anthocyanins, flavonoids, etc., and keeping the original color tone well. In addition, there is a feature of masking unpleasant tastes such as bitterness and astringency.

【００１３】本発明の活性酸素消去能の低減を抑制した
組成物には、活性酸素消去能を有する植物性可食物、植
物性抗酸化物質及びトレハロースに加えて、必要に応じ
て、更に、必須ミネラル、食物繊維、ビフィズス菌増殖
促進糖質、ビタミン、生理活性物質及び保存料等から選
ばれる１種又は２種以上の適宜の物質を固状又は溶液状
で含有せしめることも有利に実施できる。The composition of the present invention in which the reduction of the active oxygen scavenging ability is suppressed is contained, if necessary, in addition to a plant edible substance having an active oxygen scavenging ability, a plant antioxidant and trehalose. One or more appropriate substances selected from minerals, dietary fiber, bifidobacterium growth promoting carbohydrates, vitamins, physiologically active substances, preservatives, and the like can be advantageously contained in a solid or solution form.

【００１４】必須ミネラルとしては、例えば、カルシウ
ム、マグネシウム、リン、鉄、銅、亜鉛、コバルト等か
ら選ばれるミネラルを適量使用すればよい。食物繊維と
しては、例えば、ペクチン、アルギン酸、カラゲナン、
アラビアガム、グルコマンナン、シクロデキストリン、
プルラン等が有利に利用できる。とりわけ、プルラン及
び／又はシクロデキストリンは、本発明のトレハロース
と併用することにより、植物由来の活性酸素消去能の低
減抑制に著しい効果を発揮することが判明し、活性酸素
消去能の低減を抑制した組成物の製造にトレハロースと
ともに有利に利用できる。As the essential mineral, for example, an appropriate amount of a mineral selected from calcium, magnesium, phosphorus, iron, copper, zinc, cobalt and the like may be used. As a dietary fiber, for example, pectin, alginic acid, carrageenan,
Gum arabic, glucomannan, cyclodextrin,
Pullulan or the like can be advantageously used. In particular, it was found that pullulan and / or cyclodextrin, when used in combination with the trehalose of the present invention, exert a remarkable effect in suppressing the reduction of plant-derived active oxygen scavenging ability, and suppressed the reduction of active oxygen scavenging ability. It can be advantageously used with trehalose in the manufacture of the composition.

【００１５】ビフィズス菌増殖糖質としては、例えば、
ラクトスクロース、フラクトオリゴ糖、ガラクトオリゴ
糖、イソマルトオリゴ糖等が、必要に応じて適宜用いら
れる。Examples of the bifidobacterium-grown carbohydrate include, for example,
Lactosucrose, fructooligosaccharides, galactooligosaccharides, isomaltooligosaccharides and the like are appropriately used as needed.

【００１６】ビタミンとしては、例えば、チアミン、リ
ボフラビン、Ｌ−アスコルビン酸、ルチン、ヘスペリジ
ン、ナリンジン、ナイアシン、ピリドキシン、シアノコ
バラミン等の水溶性ビタミン若しくはこれらの誘導体
や、ビタミンＡ、ビタミンＤ、α−トコフェロール、ビ
タミンＫ等の脂溶性ビタミン若しくはこれらの誘導体等
が有利に用いられる。Examples of the vitamin include water-soluble vitamins such as thiamine, riboflavin, L-ascorbic acid, rutin, hesperidin, naringin, niacin, pyridoxine, cyanocobalamin and derivatives thereof, vitamin A, vitamin D, α-tocopherol, Fat-soluble vitamins such as vitamin K or derivatives thereof are advantageously used.

【００１７】生理活性物質としては、例えば、ホルモ
ン、抗菌物質、サイトカイン、プロポリス等の１種又は
２種以上を、必要に応じて利用することも随意である。
保存料としては、例えば、エタノール、酢酸、乳酸、食
塩等の適量を、必要に応じて利用することも随意であ
る。As the physiologically active substance, for example, one or more of hormones, antibacterial substances, cytokines, propolis and the like can be optionally used as needed.
As a preservative, for example, an appropriate amount of ethanol, acetic acid, lactic acid, salt, or the like can be optionally used as needed.

【００１８】更に、本発明の組成物や本発明の組成物を
含有せしめた製品の品質を向上させるために、必要に応
じて、適宜な着香料、着色料、呈味料、安定剤、増量剤
等を併用することも有利に実施できる。Further, in order to improve the quality of the composition of the present invention or a product containing the composition of the present invention, if necessary, appropriate flavors, coloring agents, flavoring agents, stabilizers, and bulking agents may be used. The use of an agent or the like can be advantageously carried out.

【００１９】また、本発明によれば、トレハロースを有
効成分とする活性酸素消去能低減抑制剤を、植物由来の
活性酸素消去能を有する物質に含有せしめて該活性酸素
消去能の低減を抑制することも有利に実施できる。活性
酸素消去能低減抑制剤としてのトレハロースの含量は、
植物由来の活性酸素消去能の低減を抑制させる効果が発
揮できればよく、通常、該抑制剤に対して、無水物換算
で、約１０％以上、望ましくは約２０％以上、更に望ま
しくは約５０％以上が好適である。本発明の活性酸素消
去能低減抑制剤は、有効成分であるトレハロースのみか
ら構成されてもよいし、必要に応じて、前述の必須ミネ
ラル、食物繊維、ビフィズス菌増殖促進糖質、ビタミ
ン、生理活性物質及び保存料等他の物質から選ばれる１
種又は２種以上を併用することも随意である。とりわ
け、プルラン及び／又はシクロデキストリンは、有効成
分であるトレハロースと併用することにより、植物由来
の活性酸素消去能の低減抑制効果に著しい効果を発揮す
ることが判明し、活性酸素消去能の低減を抑制した組成
物の製造に有利に利用できる。Further, according to the present invention, the active oxygen scavenging ability reduction inhibitor containing trehalose as an active ingredient is contained in a plant-derived substance having an active oxygen scavenging ability to suppress the reduction of the active oxygen scavenging ability. This can also be carried out advantageously. The content of trehalose as an active oxygen scavenging ability reduction inhibitor is
It is sufficient that the effect of suppressing the reduction of the ability to scavenge active oxygen derived from plants can be exerted. Usually, about 10% or more, preferably about 20% or more, and more preferably about 50% of the inhibitor in terms of anhydride. The above is preferable. The active oxygen scavenging ability reduction inhibitor of the present invention may be composed only of trehalose as an active ingredient, or if necessary, the above-mentioned essential minerals, dietary fiber, bifidobacterium growth promoting carbohydrate, vitamins, and physiological activities. 1 selected from other substances such as substances and preservatives
A combination of two or more species is also optional. In particular, pullulan and / or cyclodextrin, when used in combination with trehalose as an active ingredient, have been found to exert a remarkable effect on the reduction of plant-derived active oxygen scavenging ability. It can be used to advantage in the production of controlled compositions.

【００２０】このようにして得られる活性酸素消去能低
減抑制剤は、その形状を問わず、例えば、シラップ、粉
末、結晶、顆粒、錠剤等いずれの形状であってもよい。
また、本発明の活性酸素消去能低減抑制剤は、植物性可
食物等が持つ活性酸素消去能の低減抑制効果が発揮でき
ればよく、その使用方法は問わない。例えば、前述のト
レハロースを含有せしめる植物由来の活性酸素消去能低
減抑制方法に準じて使用すればよい。具体的には、植物
性可食物及び／又は植物性抗酸化物質に対する有効成分
であるトレハロースの使用量も、また使用方法、使用条
件等についても、前述のトレハロースを含有せしめる活
性酸素消去能低減抑制方法と同様に行えば、植物由来の
活性酸素消去能の低減抑制効果を有利に発揮でき、活性
酸素消去能低減を抑制した各種組成物を容易に調製する
ことができる。The active oxygen scavenging ability reduction inhibitor thus obtained may be in any form, such as syrup, powder, crystal, granule, tablet, etc., regardless of its shape.
Further, the active oxygen scavenging ability reduction inhibitor of the present invention is not limited as long as it can exert the effect of suppressing the reduction of the active oxygen scavenging ability of plant edible foods and the like. For example, the trehalose may be used in accordance with the above-described method for suppressing the reduction of the ability to eliminate active oxygen derived from plants. More specifically, the amount of trehalose, which is an active ingredient against plant edible foods and / or plant antioxidants, and the method of use and conditions of use, are also controlled to reduce the active oxygen scavenging ability by containing trehalose. If carried out in the same manner as the method, the effect of suppressing the reduction of the plant-derived active oxygen scavenging ability can be advantageously exhibited, and various compositions in which the reduction of the active oxygen scavenging ability can be suppressed can be easily prepared.

【００２１】以上述べてきたようにして得られた本発明
の組成物は、後述の実験１で説明するニトロブルーテト
ラゾリウム法で、１ｇ当たり５単位以上、望ましくは１
０単位以上の活性酸素消去能を有し、経時変化も少な
く、安定した活性酸素消去能を経口、経管、経皮、経粘
膜等適宜の経路により容易に利用することができる。本
発明の植物性可食物含有組成物の摂取は、生体にとっ
て、植物性可食物が本来有するビタミン、ミネラル、食
物繊維等の機能性成分に加えて、新たに、活性酸素消去
能を強化することとなり、健康の維持増進、老化防止、
成人病の予防、難病の治療促進、発癌の抑制等に好都合
であり、飲食物、化粧品、医薬品、それらの原材料又は
加工中間物等として有利に利用できる。The composition of the present invention obtained as described above is obtained by the nitro blue tetrazolium method described in Experiment 1 described below, and the unit is preferably 5 units or more, preferably 1 unit.
It has an active oxygen scavenging ability of 0 units or more, has little change with time, and can easily utilize a stable active oxygen scavenging ability by an appropriate route such as oral, transluminal, transdermal or transmucosal. Ingestion of the plant edible food-containing composition of the present invention, for the living body, in addition to the functional components such as vitamins, minerals, and dietary fiber inherent in the plant edible food, newly enhance the ability to scavenge active oxygen. Maintenance and promotion of health, prevention of aging,
It is advantageous for prevention of adult diseases, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like, and can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, raw materials or processed intermediates thereof, and the like.

【００２２】本発明の組成物の用途については、各種健
康飲食物への利用はもとより、一般的飲食物としても、
例えば、ふりかけ、ソース、ケチャップ、焼肉のタレ、
カレールウ、スープの素等の調味料、もち、だんご、キ
ャンディー、チューインガム、焼菓子、スナック菓子、
ワッフル、スポンジ菓子、パン等の各種和洋菓子類、ア
イスクリーム、アイスキャンディー等氷菓、フルーツペ
ースト、フルーツソース、ピーナッツペースト、生ジャ
ム類、漬物、ソーセージ、ハム等畜肉製品、かまぼこ、
ちくわ、はんぺん、ソーセージ等魚肉製品、珍味、佃
煮、惣菜、酒類、青汁、豆乳、ジュース、炭酸飲料等清
涼飲料、おかゆ、雑炊、炊込御飯等米飯類、ひやむぎ、
スパゲティー、マカロニ、パスタ等麺類、スナック食
品、プリンミックス、ホットケーキミックス、即席ジュ
ース、即席スープ、冷凍食品等の飲食物、それらの原材
料又は加工中間物等として品質改良、呈味改良、嗜好性
改善、栄養強化等の目的で有利に利用される。また、化
粧品としては、美肌剤、パック剤、クリーム、シャンプ
ー、リンス、育毛剤、浴用剤、酵素剤、練歯磨等に、例
えば、液剤、ペースト剤、粉剤、顆粒剤、それらの原材
料又は加工中間物等として有利に利用される。更に、医
薬品としては、経口及び／又は経管栄養剤、治療剤、イ
ンターフェロン誘導剤等に、例えば、液剤、ペースト
剤、顆粒剤、錠剤、カプセル剤又はそれらの原材料等と
して有利に利用され、また、経皮、経粘膜栄養剤、治療
剤等に、例えば点眼剤、点鼻剤、消化剤、健胃剤、酵素
剤、軟膏、パップ剤、それらの原材料又は加工中間物等
として前述の老化に伴う各種疾患の治療、予防等に有利
に利用される。The composition of the present invention can be used not only for various health foods but also for general foods and drinks.
For example, sprinkle, sauce, ketchup, grilled meat sauce,
Seasonings such as curry roux, soup ingredients, rice cake, dumpling, candy, chewing gum, baked confectionery, snack confectionery,
Various Japanese and Western sweets such as waffles, sponge sweets, bread, ice cream, ice candy, ice paste, fruit paste, fruit sauce, peanut paste, raw jams, pickles, sausage, ham and other meat products, kamaboko,
Fish products such as chikuwa, hampan, sausage, delicacy, boiled foods such as boiled soybeans, prepared dishes, alcoholic beverages, green juice, soy milk, juice, carbonated beverages, rice porridge, porridge, rice cooked rice such as cooked rice, hiyamugi,
Spaghetti, macaroni, pasta noodles, snack foods, pudding mixes, hot cake mixes, instant juices, instant soups, foods and drinks such as frozen foods, quality improvement, taste improvement, taste improvement as raw materials or processed intermediates etc. It is advantageously used for the purpose of nutrition enhancement. In addition, cosmetics include beautiful skin agents, packs, creams, shampoos, rinses, hair restorers, bath agents, enzyme agents, toothpastes, etc., for example, liquids, pastes, powders, granules, their raw materials or processing intermediates It is advantageously used as an object. Further, as a pharmaceutical, it is advantageously used as an oral and / or tube feeding, a therapeutic agent, an interferon inducer, etc., for example, as a liquid, a paste, a granule, a tablet, a capsule or a raw material thereof, , Percutaneous, transmucosal nutrients, therapeutic agents, etc., such as eye drops, nasal drops, digestive agents, stomachic, enzyme agents, ointments, cataplasms, their raw materials or processed intermediates, etc. It is advantageously used for treatment and prevention of diseases.

【００２３】本発明の活性酸素消去能の低減を抑制した
組成物は、基本的には、食品材料から構成され毒性を懸
念することなく利用でき、これを経口摂取する場合、通
常、大人、一日当たり、無水物換算で、約０．１乃至１
０００ｇ、望ましくは約０．２乃至５００ｇ、更に望ま
しくは約１乃至１００ｇ、の範囲で利用するのが好適で
ある。経管、経皮、経粘膜等の他の経路による利用の場
合も、経口摂取の場合の量に準じて利用すればよい。The composition of the present invention in which the ability to scavenge active oxygen is suppressed is basically composed of food materials and can be used without concern about toxicity. About 0.1 to 1 in terms of anhydride per day
It is suitable to use in the range of 000 g, preferably about 0.2 to 500 g, more preferably about 1 to 100 g. In the case of use by other routes such as tube, transdermal, transmucosal, etc., it may be used according to the amount of oral ingestion.

【００２４】以下、本発明を実験で詳細に説明する。Hereinafter, the present invention will be described in detail by experiments.

【００２５】[0025]

【実験１】〈活性酸素消去能の低減抑制に与える各種糖
質の影響〉生の人参をミキサーにかけて破砕し、この破
砕物に対して、各種糖質を無水糖質重量に換算して１０
％混合溶解し、４０℃で２日間減圧乾燥の後、同じ温度
で２４時間真空乾燥し、ミキサーで粉砕し粉末状人参含
有組成物を調製した。使用した糖質は、グルコース、マ
ンニトール、ソルビトール、マルトース、スクロース、
トレハロース及びプルランでいずれも市販の試薬を使用
した。調製した粉末状組成物約１００ｇを、５００ｍｌ
容のポリスチレン製容器に入れ密封して、４０℃で７日
間保存したときの活性酸素消去能を保存開始時のそれと
比較した。活性酸素消去能の測定は、ニトロブルーテト
ラゾリウム（ＮＢＴ）法［今成登志男ら，医学のあゆ
み，１０１，４９６−４９７（１９７７）］に従い、キ
サンチン−キサンチンオキシダーゼ系で生成するスーパ
ーオキシド量を、ＮＢＴを還元して生ずるホルマザン量
を比色定量して測定する方法により行った。対照として
精製水を試験液とした。対照と比較してホルマザン生成
量を５０％阻害する活性を活性酸素消去能の活性１単位
と定義した。調製した粉末状組成物の組成と保存開始前
後の組成物１ｇ当りの活性酸素消去能を表１にまとめ
た。表１より明らかなように、試験した糖質の中で、ト
レハロースを使用したものが最も活性酸素消去能の残存
率が高く、トレハロースが活性酸素消去能の低減抑制に
著効を示すことが判明した。トレハロースの活性酸素消
去能の低減抑制効果は、トレハロースの構成糖であるグ
ルコースや、トレハロースに類似した、２つのグルコー
ス単位からなる２糖類のマルトースよりも著しく強いも
のであった。従って、トレハロースは活性酸素消去能低
減抑制剤の有効成分として好適である。また、トレハロ
ースに次いで、プルランも活性酸素消去能の低減抑制効
果が高かった。[Experiment 1] <Effects of various carbohydrates on suppression of reduction of active oxygen scavenging ability> Raw ginseng was crushed with a mixer, and various saccharides were converted to anhydrous carbohydrate weight based on this crushed product.
%, And dried under reduced pressure at 40 ° C. for 2 days, followed by vacuum drying at the same temperature for 24 hours, and pulverized with a mixer to prepare a powdered ginseng-containing composition. The carbohydrates used were glucose, mannitol, sorbitol, maltose, sucrose,
Commercial reagents were used for both trehalose and pullulan. About 100 g of the prepared powdery composition was added to 500 ml
The storage capacity at 40 ° C. for 7 days was sealed in a polystyrene container, and the ability to scavenge active oxygen was compared with that at the start of storage. According to the nitro blue tetrazolium (NBT) method [Toshio Imanari et al., History of Medicine, 101, 496-497 (1977)], the amount of superoxide generated in a xanthine-xanthine oxidase system was measured by measuring the reactive oxygen scavenging ability. The amount of formazan produced by reduction of NBT was measured by colorimetry. Purified water was used as a test liquid as a control. The activity that inhibits the amount of formazan produced by 50% as compared with the control was defined as one unit of the activity of scavenging active oxygen. Table 1 summarizes the composition of the prepared powdery composition and the ability to scavenge active oxygen per gram of the composition before and after the start of storage. As is clear from Table 1, among the carbohydrates tested, those using trehalose have the highest residual rate of active oxygen scavenging ability, and it is found that trehalose is remarkably effective in suppressing reduction of active oxygen scavenging ability. did. The effect of trehalose on inhibiting the ability to scavenge active oxygen was significantly stronger than glucose, a constituent sugar of trehalose, or maltose, a disaccharide composed of two glucose units similar to trehalose. Therefore, trehalose is suitable as an active ingredient of the active oxygen scavenging ability reduction inhibitor. In addition, pullulan, after trehalose, also had a high effect of suppressing the reduction of active oxygen scavenging ability.

【００２６】[0026]

【表１】 [Table 1]

【００２７】[0027]

【実験２】〈活性酸素消去能の低減抑制に与えるトレハ
ロース濃度の影響〉実験１と同様に、人参破砕物に対し
て、トレハロースを無水糖質重量に換算して０乃至２０
％量混合溶解し、４０℃で２４時間真空乾燥、粉砕を行
い、粉末状人参含有組成物を調製した。これら粉末状組
成物を、実験１と同様に、ポリスチレン製容器に入れ密
封し、４０℃で７日間保存し、活性酸素消去能の低減抑
制効果を調べた。調製した粉末状組成物の組成と保存前
後の組成物１ｇ当りの活性酸素消去能を表２に示す。ト
レハロース共存量が、無水物換算で約１％以上、望まし
くは約５％以上が、更に望ましくは２０％以上が活性酸
素消去能の低減抑制効果に優れていた。[Experiment 2] <Effect of trehalose concentration on suppression of reduction of active oxygen scavenging ability> As in Experiment 1, trehalose was converted to an anhydrous sugar weight of crushed ginseng in an amount of 0 to 20%.
%, Mixed and dissolved, vacuum dried and crushed at 40 ° C. for 24 hours to prepare a powdered ginseng-containing composition. These powdery compositions were sealed in a polystyrene container and stored at 40 ° C. for 7 days in the same manner as in Experiment 1, and the effect of suppressing the reduction of the active oxygen scavenging ability was examined. Table 2 shows the composition of the prepared powdery composition and the ability to scavenge active oxygen per gram of the composition before and after storage. When the trehalose coexistence amount was about 1% or more, preferably about 5% or more, more preferably 20% or more in terms of anhydride, the effect of suppressing the reduction of the active oxygen scavenging ability was excellent.

【００２８】[0028]

【表２】 [Table 2]

【００２９】[0029]

【実験３】〈各種野菜の活性酸素消去能の低減抑制に与
えるトレハロースの影響〉各種野菜について、実験１と
同様に処理し、野菜破砕物に対して、トレハロースを無
水糖質重量に換算して１０％添加し、４５℃で２０時間
真空乾燥、粉砕して粉末状野菜含有組成物を調製し、こ
れらを実験１と同様に容器に入れ、４０℃で６日間保存
した。保存前後の組成物１ｇ当りの活性酸素消去能を、
トレハロース不使用のものと比較した。調製した粉末状
組成物の組成と活性酸素消去能を表３に示す。人参、玉
ねぎ、大根、キャベツ、ほうれん草、きゅうり、かぼち
ゃ等多くの野菜において、トレハロースを含有せしめる
ことで活性酸素消去能の低減抑制効果が認められた。[Experiment 3] <Effect of trehalose on suppression of reduction of active oxygen scavenging ability of various vegetables> Various vegetables are treated in the same manner as in Experiment 1, and trehalose is converted to anhydrous sugar weight of the crushed vegetable. 10% was added, vacuum-dried at 45 ° C. for 20 hours, and pulverized to prepare a powdered vegetable-containing composition, which was placed in a container as in Experiment 1, and stored at 40 ° C. for 6 days. The ability to scavenge active oxygen per gram of the composition before and after storage,
Compared to those without trehalose. Table 3 shows the composition and the active oxygen scavenging ability of the prepared powdery composition. In many vegetables such as carrot, onion, radish, cabbage, spinach, cucumber, and pumpkin, the effect of suppressing the reduction of active oxygen scavenging ability was confirmed by adding trehalose.

【００３０】[0030]

【表３】 [Table 3]

【００３１】[0031]

【実験４】〈トレハロースによるスーパーオキシド・ジ
スムターゼ及び各種抗酸化物質に対する活性酸素消去能
の低減抑制効果〉濃度４５％トレハロース水溶液を用い
て、この１ｍｌ当たりに、西洋わさび由来スーパーオキ
シド・ジスムターゼ（シグマ社製）を５μｇ，α−グル
コシルヘスペリジン（（株）林原生物化学研究所製）を
１０ｍｇ，α−グルコシルルチン（（株）林原生物化学
研究所製）を１０ｍｇ，没食子酸（和光純薬工業製）を
２５０μｇ及びカテキン（和光純薬工業製）を５０μｇ
をそれぞれ溶解して液状組成物を得、これらをそれぞれ
ガラス製試験管に入れゴム栓で密封し、遮光下で保存し
た。対照として、前記トレハロース水溶液の代わりに水
を用いた。それらの保存条件と保存前後の活性酸素消去
能を表４に示す。表４に示すごとく、スーパーオキシド
・ジスムターゼの他、α−グルコシルヘスペリジン，α
−グルコシルルチン，没食子酸及びカテキン等植物由来
の抗酸化物質の有する活性酸素消去能についても、トレ
ハロースが著しい低減抑制効果を発揮することが認めら
れた。また、別に、トレハロース含水結晶粉末と各抗酸
化物質粉末とをそれぞれ混合粉末とし、これらを同様に
保存試験したが、いずれも活性酸素消去能についてほと
んど変化が見られなかった。このことから、トレハロー
スは、粉末状で混合しただけではその効果を発揮しにく
く、トレハロースが溶けた状態で接触含有、換言すれば
水性媒体で含有せしめることによりその効果を発揮する
ことが判明した。[Experiment 4] <Effect of trehalose on reducing the ability to scavenge active oxygen for superoxide dismutase and various antioxidants> Using a 45% trehalose aqueous solution, per 1 ml of this was added horseradish-derived superoxide dismutase (Sigma). 5 μg, α-glucosyl hesperidin (manufactured by Hayashibara Biochemical Laboratory) 10 mg, α-glucosylrutin (manufactured by Hayashibara Biochemical Laboratory) 10 mg, gallic acid (manufactured by Wako Pure Chemical Industries) 250 μg and 50 μg of catechin (manufactured by Wako Pure Chemical Industries)
Were respectively dissolved to obtain liquid compositions, each of which was placed in a glass test tube, sealed with a rubber stopper, and stored under light shielding. As a control, water was used instead of the trehalose aqueous solution. Table 4 shows their storage conditions and the ability to scavenge active oxygen before and after storage. As shown in Table 4, in addition to superoxide dismutase, α-glucosyl hesperidin, α
-Regarding the active oxygen scavenging ability of antioxidants derived from plants such as glucosyl rutin, gallic acid and catechin, it was also recognized that trehalose exerts a remarkable reduction suppressing effect. Separately, the trehalose hydrated crystal powder and each antioxidant substance powder were separately prepared as mixed powders, and they were similarly subjected to a storage test. However, no change was observed in any of the active oxygen scavenging ability. From this, it has been found that trehalose is hard to exhibit its effect only by mixing it in a powder form, and it is found that the effect is exhibited by containing trehalose in contact with the trehalose in a dissolved state, in other words, by incorporating it in an aqueous medium.

【００３２】[0032]

【表４】 [Table 4]

【００３３】[0033]

【実験５】〈薬草抽出物に対する活性酸素消去能の低減
抑制効果〉おおばこ乾燥物（車前草、局方、大同製薬
製）５００ｇに１０ｌの水を加え、９０分間沸騰させ煎
じた。バスケット型遠心機で煎液を回収した。煎液を更
に煮詰め、濃度約５％液を２ｋｇ得た。この煮詰め液１
ｋｇに対して、トレハロース及びプルランを無水糖質重
量に換算して、それぞれ２４０ｇ及び１０ｇを混合溶解
し、次いで、卓上型噴霧乾燥機（ＳＤ−１、東京理化
製）にて噴霧乾燥し、粉末状おおばこ抽出物含有組成物
を調製した。対照として、トレハロース及びプルランに
代えて、デキストリン（ＤＥ約１１）を用いて、同様の
粉末状組成物を調製した。これら粉末状組成物を、実験
１と同様に、ポリスチレン容器に入れ密封して４０℃で
７日間保存し、活性酸素消去能の低減抑制効果を調べ
た。保存開始時、共に１ｇ当たり約５０００単位であっ
たのに対し、７日間保存した後には、トレハロース及び
プルランを用いた組成物が１ｇ当たり約４３００単位で
あったのに対し、デキストリンを使用した組成物は１ｇ
当たり約３１００単位であった。従って、トレハロース
及びプルランの使用は、デキストリンの場合と比較し
て、明らかに、活性酸素消去能の低減抑制効果に優れて
いる。[Experiment 5] <Effect of reducing active oxygen scavenging ability on herbal extract> 10 l of water was added to 500 g of dried sardine (car foreground grass, Pharmacopoeia, manufactured by Daido Pharmaceutical Co., Ltd.), and the mixture was boiled and boiled for 90 minutes. The decoction was collected with a basket-type centrifuge. The decoction was further boiled down to obtain 2 kg of a liquid having a concentration of about 5%. This boiled liquid 1
Trehalose and pullulan were converted to anhydrous carbohydrate weight, and 240 g and 10 g, respectively, were mixed and dissolved, and then spray-dried with a desktop spray dryer (SD-1, manufactured by Tokyo Rika) to obtain a powder. A composition containing the leafy tobacco extract was prepared. As a control, a similar powdery composition was prepared using dextrin (DE about 11) instead of trehalose and pullulan. These powdery compositions were sealed in a polystyrene container and stored at 40 ° C. for 7 days in the same manner as in Experiment 1, and the effect of suppressing the reduction of the active oxygen scavenging ability was examined. At the start of storage, both were about 5000 units per gram, and after storage for 7 days, the composition using trehalose and pullulan was about 4300 units per gram, whereas the composition using dextrin was Thing is 1g
About 3100 units per unit. Therefore, the use of trehalose and pullulan is clearly superior to the effect of dextrin in suppressing the reduction of active oxygen scavenging ability.

【００３４】以下、若干の実施例によって本発明を説明
する。実施例Ａで本発明の活性酸素消去能低減抑制剤
を、実施例Ｂで、活性酸素消去能の低減を抑制した組成
物を説明する。Hereinafter, the present invention will be described with reference to some examples. Example A describes the active oxygen scavenging ability reduction inhibitor of the present invention, and Example B describes a composition in which the reduction of active oxygen scavenging ability is suppressed.

【００３５】[0035]

【実施例Ａ−１】〈活性酸素消去能低減抑制剤〉とうも
ろこし澱粉を濃度約３３％の澱粉乳とし、これに炭酸カ
ルシウム０．１％加え、ｐＨ６．５に調整し、α−アミ
ラーゼ（ターマミル、ノボ製）を澱粉ｇ当たり０．３％
加え、９５℃で１５分間反応させ、次いで、１２０℃に
オートクレーブし、急冷してＤＥ約４の液化溶液を得、
次いで、特開平７−２１３２８３号公報に開示される方
法でリゾビウム属に属する微生物を培養して調製した非
還元性糖質生成酵素とトレハロース遊離酵素とを澱粉１
ｇ当たりそれぞれ４単位、イソアミラーゼを澱粉１ｇ当
たり５００単位及びシクロマルトデキストリン・グルカ
ノトランスフェラーゼを澱粉１ｇ当たり５単位加え、ｐ
Ｈ６．２、温度４０℃で４８時間反応させた。本反応液
を加熱失活し、次いでグルコアミラーゼを基質１ｇ当た
り１０単位加え、ｐＨ５．０、温度５０℃で１０時間反
応させた。本反応液は、無水物換算でトレハロースを約
８６％含有していた。本反応液を加熱失活し、常法に従
って、脱色、脱塩して精製し、濃縮しながら連続晶析さ
せ、得られるマスキットをバスケット型遠心分離器で分
蜜し、結晶を少量の水でスプレーし洗浄して純度９８％
以上のトレハロース含水結晶を無水物換算で約６４％の
収率で得た。本品は、極めて純度の高いトレハロース含
水結晶であって、本発明の活性酸素消去能低減抑制剤と
して好適であり、植物性可食物等の活性酸素消去能の低
減抑制に有利に利用できる。Example A-1 <Active oxygen scavenging ability reduction inhibitor> Corn starch was made into a starch milk having a concentration of about 33%, calcium carbonate was added to 0.1%, pH was adjusted to 6.5, and α-amylase (termamyl) was added. 0.3% per g of starch
In addition, the mixture was reacted at 95 ° C. for 15 minutes, then autoclaved to 120 ° C., and quenched to obtain a liquefied solution having a DE of about 4,
Then, a non-reducing saccharide-forming enzyme and a trehalose-releasing enzyme prepared by culturing a microorganism belonging to the genus Rhizobium according to the method disclosed in Japanese Patent Application Laid-Open No. 7-213283 are combined with starch 1
4 units per gram, 500 units of isoamylase per gram of starch and 5 units of cyclomaltodextrin / glucanotransferase per gram of starch.
The reaction was performed at H6.2 at a temperature of 40 ° C. for 48 hours. This reaction solution was heated and inactivated, and then 10 units of glucoamylase was added per 1 g of the substrate, and reacted at pH 5.0 and a temperature of 50 ° C. for 10 hours. This reaction solution contained about 86% of trehalose in terms of anhydride. The reaction solution is deactivated by heating, decolorized, desalted, purified and continuously crystallized while concentrating according to a conventional method.The resulting mass kit is separated by a basket-type centrifugal separator, and the crystals are washed with a small amount of water. Spray, wash and 98% pure
The above hydrated trehalose crystals were obtained in a yield of about 64% in terms of anhydride. This product is an extremely pure trehalose hydrated crystal, and is suitable as the active oxygen scavenging ability reduction inhibitor of the present invention, and can be advantageously used for suppressing the reduction of active oxygen scavenging ability of vegetable edible foods and the like.

【００３６】[0036]

【実施例Ａ−２】〈活性酸素消去能低減抑制剤〉特開平
７−１７０９７７号公報に開示されている方法で、マル
トース・トレハロース変換酵素産生能を有するサーマス
属に属する微生物を培養し、遠心分離して得た湿菌体２
５０ｇ（約７５００単位）をアルギン酸ナトリウム溶液
と塩化カルシウム溶液とを用いて、アルギン酸固定化菌
体とし、本固定化菌体を、直径５．４ｃｍ、長さ１００
ｃｍのジャケット付きガラス製カラムに充填し、温度を
６０℃に保温した。この固定化カラムに、ｐＨ６．５の
濃度４０％のマルトース溶液を、ＳＶ０．２にて下降法
で通液して、トレハロース約６６％、マルトース約２８
％及びグルコース約６％の組成を有する反応液を得た。
本溶液を、常法に従って、脱色、脱塩して精製し、減圧
濃縮して濃度約７５％にし、次いで、これを助晶機にと
り、種晶としてトレハロース含水結晶及びマルトース含
水結晶をそれぞれ約１％ずつ加えて徐冷し、晶出率約２
５％のマスキットを得た。本マスキットを噴霧乾燥し、
熟成して結晶性粉末糖質を原料のマルトースに対して、
無水物換算で約９２％の収率で得た。本品は、無水物換
算でトレハロース約６６％、マルトース約２８％及びグ
ルコース約６％の組成を有する結晶化度約６０％の安定
な粉末糖質で、活性酸素消去能低減抑制剤として好適で
あり、植物性可食物等の活性酸素消去能の低減抑制に有
利に利用できる。Example A-2 <Active oxygen scavenging ability reduction inhibitor> A microorganism belonging to the genus Thermus having maltose / trehalose converting enzyme-producing ability was cultured by the method disclosed in Japanese Patent Application Laid-Open No. 7-170977, and centrifuged. Wet cells 2 obtained by separation
Alginate-immobilized cells were prepared using 50 g (about 7500 units) of a sodium alginate solution and a calcium chloride solution. The immobilized cells were 5.4 cm in diameter and 100 cm in length.
The column was packed in a glass column with a jacket and a temperature of 60 ° C. A maltose solution having a pH of 6.5 and a concentration of 40% was passed through the immobilized column at a SV of 0.2 by a descending method to give about 66% trehalose and about 28% maltose.
% And about 6% glucose.
The solution was purified by decolorization, desalting, and concentrated under reduced pressure to a concentration of about 75% according to a conventional method. Then, the solution was placed in an auxiliary crystallizer, and hydrated trehalose crystals and hydrated maltose crystals were each used as seed crystals. % And gradually cooled, and the crystallization rate was about 2
A 5% mass kit was obtained. Spray dry this mask kit,
Aging and adding crystalline powdered sugar to maltose as raw material,
It was obtained in a yield of about 92% in terms of anhydride. This product is a stable powdered carbohydrate having a composition of about 66% trehalose, about 28% maltose and about 6% glucose in terms of anhydride and having a crystallinity of about 60%, and is suitable as an active oxygen scavenging ability reduction inhibitor. Yes, it can be advantageously used to suppress the reduction of the ability to scavenge active oxygen such as vegetable edibles.

【００３７】[0037]

【実施例Ａ−３】〈活性酸素消去能低減抑制剤〉馬鈴薯
澱粉を濃度１０％の澱粉乳とし、これにα−アミラーゼ
を作用させて液化溶液を得、次いで、特開平７−２１３
２８３号公報に開示した非還元性糖質生成酵素を澱粉１
ｇ当たり３単位及びトレハロース遊離酵素を澱粉１ｇ当
たり５単位、イソアミラーゼを澱粉１ｇ当たり１０００
単位及びマルトテトラオース生成アミラーゼを１単位加
え、ｐＨ６．０、温度４０℃で４８時間反応させた。本
反応液を加熱して酵素を失活させた後、常法に従って、
脱色、脱塩して精製し、減圧濃縮して水分約３０％、Ｄ
Ｅ約１５のシラップを無水物換算で収率約９０％で得
た。本品は、無水物換算でトレハロースを約５０％とと
もに他の澱粉由来糖質を含有する安定なシラップで、活
性酸素消去能低減抑制剤として好適であり、植物性可食
物等の活性酸素消去能の低減抑制に有利に利用できる。Example A-3 <Inhibitor for reducing active oxygen scavenging ability> Potato starch was converted to starch milk having a concentration of 10%, and α-amylase was allowed to act on the starch milk to obtain a liquefied solution.
No. 283, the non-reducing saccharide-forming enzyme disclosed in
3 units / g and trehalose-releasing enzyme 5 units / g of starch, isoamylase 1000 / g of starch
Unit and 1 unit of maltotetraose-forming amylase were added, and the mixture was reacted at pH 6.0 at a temperature of 40 ° C. for 48 hours. After heating the reaction solution to deactivate the enzyme,
Decolorized, desalted and purified, concentrated under reduced pressure to a water content of about 30%, D
A syrup having an E of about 15 was obtained in a yield of about 90% in terms of anhydride. This product is a stable syrup containing about 50% trehalose in terms of anhydride and other starch-derived carbohydrates, and is suitable as an inhibitor for reducing active oxygen scavenging ability. Can be advantageously used to suppress the reduction of

【００３８】[0038]

【実施例Ａ−４】〈活性酸素消去能低減抑制剤〉実施例
Ａ−１の方法で得た高純度トレハロース含水結晶１００
重量部に、プルラン１重量部を均一に混合して、固状の
活性酸素消去能低減抑制剤を製造した。本品は、植物性
可食物等の活性酸素消去能の低減抑制に有利に利用でき
る。Example A-4 <Active oxygen scavenging ability reduction inhibitor> High purity trehalose hydrated crystal 100 obtained by the method of Example A-1
1 part by weight of pullulan was uniformly mixed with 1 part by weight of a pullulan to prepare a solid active oxygen scavenging ability reduction inhibitor. The product can be advantageously used for suppressing the reduction of the ability to scavenge active oxygen such as vegetable edibles.

【００３９】[0039]

【実施例Ａ−５】〈活性酸素消去能低減抑制剤〉実施例
Ａ−３の方法で得たトレハロース含有シラップ１００重
量部に、プルラン０．５重量部及び易水溶性シクロデキ
ストリン０．５重量部を均一に混合溶解して、シラップ
状の活性酸素消去能低減抑制剤を製造した。本品は、植
物性可食物等の活性酸素消去能の低減抑制に有利に利用
できる。Example A-5 <Active oxygen scavenging ability reduction inhibitor> 0.5 part by weight of pullulan and 0.5 part by weight of a water-soluble cyclodextrin were added to 100 parts by weight of the trehalose-containing syrup obtained by the method of Example A-3. The parts were uniformly mixed and dissolved to produce a syrup-like active oxygen scavenging ability reduction inhibitor. The product can be advantageously used for suppressing the reduction of the ability to scavenge active oxygen such as vegetable edibles.

【００４０】[0040]

【実施例Ｂ−１】〈ほうれん草含有組成物〉ほうれん草
をミキサーにかけて破砕し、この破砕物１０重量部に対
して、実施例Ａ−４の方法で得た活性酸素消去能低減抑
制剤を１重量部及びα−グルコシルルチンを０．１重量
部混合溶解し、１００℃で１０分間加熱処理し、４０℃
で２時間通風乾燥の後、４０℃で１６時間真空乾燥を行
い、粉砕機で粉砕し、活性酸素消去能が１ｇ当たり約１
２００単位の粉末状ほうれん草含有組成物を得た。本品
は、ほうれん草本来の色調をよく保っており、食欲をそ
そるものであった。本品を摂取することは、生体にとっ
て、ほうれん草が本来有するビタミン、ミネラル、食物
繊維等の機能性成分に加えて、活性酸素消去能を強化す
ることとなり、健康の維持増進、老化防止、成人病の予
防、難病の治療促進、発癌の抑制等に好都合である。従
って、飲食物、化粧品、医薬品、それらの原材料又は加
工中間物等として有利に利用できる。Example B-1 <Spinach-containing composition> Spinach is crushed in a mixer, and 1 part by weight of the active oxygen scavenging ability reducing inhibitor obtained by the method of Example A-4 is added to 10 parts by weight of the crushed material. Parts and α-glucosyl rutin were mixed and dissolved in 0.1 part by weight, and heat-treated at 100 ° C. for 10 minutes.
After drying with air for 2 hours, vacuum drying is performed at 40 ° C. for 16 hours, and the mixture is pulverized with a pulverizer.
200 units of a powdered spinach-containing composition were obtained. This product kept the original color tone of spinach well and was appetizing. Ingestion of this product will enhance the ability of the living body to scavenge active oxygen in addition to the natural ingredients of spinach, such as vitamins, minerals, and dietary fiber, and will help maintain and improve health, prevent aging, and adult diseases. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４１】[0041]

【実施例Ｂ−２】〈キャベツ含有組成物〉キャベツを切
断しブランチングした後、細断機にかけて約５ｍｍ幅に
千切りし、この千切りキャベツ１０重量部に対して実施
例Ａ−３の方法で得た活性酸素消去能低減抑制剤１重量
部及びα−グルコシルルチン０．０５重量部を混合し室
温で２時間なじませ、次いで、４０℃で２時間通風乾燥
し、更に４０℃で１６時間真空乾燥を行い、活性酸素消
去能が、１ｇ当たり約１３００単位の細片状キャベツ含
有組成物を得た。本品は、キャベツ本来の色調をよく保
っており、食欲をそそるものであった。本品を摂取する
ことは、生体にとって、キャベツが本来有するビタミ
ン、ミネラル、食物繊維等の機能性成分に加えて、活性
酸素消去能を強化することとなり、健康の維持増進、老
化防止、成人病の予防、難病の治療促進、発癌の抑制等
に好都合である。従って、飲食物、化粧品、医薬品、そ
れらの原材料又は加工中間物等として有利に利用でき
る。Example B-2 <Cabbage-containing composition> After cutting and blanching cabbage, the cabbage was shredded to a width of about 5 mm using a shredder, and 10 parts by weight of the shredded cabbage was processed by the method of Example A-3. 1 part by weight of the active oxygen scavenging ability reduction inhibitor obtained and 0.05 part by weight of α-glucosyl rutin were mixed and allowed to mix at room temperature for 2 hours, then dried at 40 ° C. for 2 hours, and further vacuumed at 40 ° C. for 16 hours. Drying was carried out to obtain a cabbage-containing composition in the form of flakes having an active oxygen scavenging ability of about 1300 units per gram. This product kept the original color tone of cabbage well and was appetizing. Ingestion of this product will enhance the ability of the cabbage to scavenge active oxygen in addition to the functional components inherent in cabbage, such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４２】[0042]

【実施例Ｂ−３】〈なす含有組成物〉なすをスライサー
にかけ水洗いし、このスライス１０重量部に対して、実
施例Ａ−１の方法で得た活性酸素消去能低減抑制剤を１
重量部混合溶解し、７０℃で２日間通風乾燥し、活性酸
素消去能が１ｇ当たり約１１０００単位の細片状なす含
有組成物を得た。本品は、なすの皮部が本来の色調をよ
く保っており、食欲をそそるものであった。本品を摂取
することは、生体にとって、なすが本来有するビタミ
ン、ミネラル、食物繊維等の機能性成分に加えて、活性
酸素消去能を強化することとなり、健康の維持増進、老
化防止、成人病の予防、難病の治療促進、発癌の抑制等
に好都合である。従って、飲食物、化粧品、医薬品、そ
れらの原材料又は加工中間物等として有利に利用でき
る。Example B-3 <Eggplant-containing composition> Eggplant was sliced in a slicer and washed with water, and 10 parts by weight of the slice was mixed with the active oxygen scavenging ability reduction inhibitor obtained by the method of Example A-1.
Parts by weight were mixed and dissolved, and dried by ventilation at 70 ° C. for 2 days to obtain a flake-form composition having an active oxygen elimination capacity of about 11,000 units per gram. In this product, the eggplant skin kept its original color tone well, and it was appetizing. Ingestion of this product will enhance the ability of active organisms to scavenge active oxygen in addition to the functional ingredients such as vitamins, minerals, and dietary fiber, which are naturally present in the living body. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４３】[0043]

【実施例Ｂ−４】〈人参含有組成物〉人参をブランチン
グした後、スライサーにかけてスライスし、このスライ
ス１０重量部に対して、実施例Ａ−４の方法で得た活性
酸素消去能低減抑制剤を１重量部混合溶解し、５０℃で
１６時間通風乾燥し活性酸素消去能が１ｇ当り約４５０
単位の細片状人参含有組成物を得た。本品は、人参本来
の色調をよく保っており、食欲をそそるものであった。
また、本品は吸湿性が低く、保存安定性に優れ、取扱い
容易であった。本品を摂取することは、生体にとって、
人参が本来有するビタミン、ミネラル、食物繊維等の機
能性成分に加えて、活性酸素消去能を強化することとな
り、健康の維持増進、老化防止、成人病の予防、難病の
治療促進、発癌の抑制等に好都合である。従って、飲食
物、化粧品、医薬品、それらの原材料又は加工中間物等
として有利に利用できる。Example B-4 <Ginseng-containing composition> After carrots were blanched, sliced by a slicer, 10 parts by weight of the slices were suppressed in reducing the active oxygen scavenging ability obtained by the method of Example A-4. One part by weight of the agent was mixed and dissolved, and air-dried at 50 ° C. for 16 hours.
A unit of ginseng-containing composition was obtained. This product kept the original color of ginseng well and was appetizing.
The product had low hygroscopicity, excellent storage stability, and was easy to handle. Ingestion of this product is
In addition to functional ingredients such as vitamins, minerals, and dietary fiber inherent in ginseng, the ability to scavenge active oxygen will be strengthened, maintaining and improving health, preventing aging, preventing adult diseases, promoting treatment for intractable diseases, and suppressing carcinogenesis. It is convenient for etc. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４４】[0044]

【実施例Ｂ−５】〈ひじき含有組成物〉乾燥ひじきを水
で戻した後、破砕機で破砕し、この破砕物１０重量部に
対して、実施例Ａ−２の方法で得た活性酸素消去能低減
抑制剤５重量部を混合溶解し、５０℃で８時間通風乾燥
した後、４０℃で真空乾燥し、粉砕して活性酸素消去能
が、１ｇ当たり約２４単位の粉末状ひじき含有組成物を
得た。本品を摂取することは、生体にとって、ひじきが
本来有するビタミン、ミネラル、食物繊維等の機能性成
分に加えて、活性酸素消去能を強化することとなり、健
康の維持増進、老化防止、成人病の予防、難病の治療促
進、発癌の抑制等に好都合である。従って、飲食物、化
粧品、医薬品、それらの原材料又は加工中間物等として
有利に利用できる。Example B-5 <Hijiki-containing composition> After the dried hijiki was returned with water, it was crushed with a crusher, and 10 parts by weight of the crushed material was treated with active oxygen obtained by the method of Example A-2. Mix and dissolve 5 parts by weight of the quenching ability reduction inhibitor, air-dry at 50 ° C. for 8 hours, vacuum dry at 40 ° C., and pulverize to obtain a powdered hijiki-containing composition having an active oxygen scavenging capacity of about 24 units per gram. I got something. Ingestion of this product will enhance the ability of active organisms to scavenge active oxygen in addition to the functional ingredients inherent in hijiki such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４５】[0045]

【実施例Ｂ−６】〈椎茸含有組成物〉干し椎茸を水で戻
して、１００℃で１５分間加熱した後、破砕機で破砕し
この破砕物１００重量部に対して、実施例Ａ−３の方法
で得た活性酸素消去能低減抑制剤１０重量部とともに茶
抽出物０．１重量部を混合溶解し、５０℃で８時間通風
乾燥した後、４０℃で真空乾燥し、粉砕して、活性酸素
消去能が、１ｇ当たり約５６０単位の粉末状椎茸含有組
成物を得た。本品を摂取することは、生体にとって、椎
茸が本来有するビタミン、ミネラル、食物繊維等の機能
性成分に加えて、活性酸素消去能を強化することとな
り、健康の維持増進、老化防止、成人病の予防、難病の
治療促進、発癌の抑制等に好都合である。従って、飲食
物、化粧品、医薬品、それらの原材料又は加工中間物等
として有利に利用できる。Example B-6 <Composition Containing Shiitake Mushroom> Dried shiitake mushrooms were returned to water, heated at 100 ° C. for 15 minutes, crushed by a crusher, and 100 parts by weight of the crushed material was used in Example A-3. 0.1 parts by weight of the tea extract was mixed and dissolved together with 10 parts by weight of the active oxygen scavenging ability reduction inhibitor obtained by the method described above, followed by air drying at 50 ° C. for 8 hours, followed by vacuum drying at 40 ° C. and pulverization. A powdery shiitake mushroom-containing composition having an active oxygen scavenging ability of about 560 units / g was obtained. Ingestion of this product will enhance the ability of active organisms to scavenge active oxygen in addition to the functional components of Shiitake mushrooms, such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４６】[0046]

【実施例Ｂ−７】〈柚含有組成物〉柚の皮を破砕機にか
けて破砕し、この破砕物を５重量部に対して、実施例Ａ
−１の方法で得た活性酸素消去能低減抑制剤１重量部を
混合溶解し、予備凍結の後、３日間凍結乾燥し、粉砕し
て、活性酸素消去能が、１ｇ当たり約１２単位の粉末状
柚含有組成物を得た。本品は、柚本来の香り、色調をよ
く保っていた。本品を摂取することは、生体にとって、
柚が本来有するビタミン、ミネラル、食物繊維等の栄養
成分に加えて、活性酸素消去能を強化することとなり、
健康の維持増進、老化防止、成人病の予防、難病の治療
促進、発癌の抑制等に好都合である。従って、飲食物、
化粧品、医薬品、それらの原材料又は加工中間物等とし
て有利に利用できる。<Example B-7><Yuzu-containingcomposition> The citron peel was crushed by a crusher, and the crushed material was used in an amount of 5 parts by weight in Example A.
1 part by weight of the active oxygen scavenging ability reduction inhibitor obtained by the method of -1 was mixed and dissolved, and after preliminary freezing, freeze-dried for 3 days and pulverized to obtain a powder having an active oxygen scavenging ability of about 12 units per gram. A yuzu-containing composition was obtained. This product kept the original aroma and color tone of Yuzu. Ingestion of this product is
In addition to nutrients such as vitamins, minerals, and dietary fiber that yuzu originally has, it will enhance the ability to eliminate active oxygen,
It is convenient for maintaining and improving health, preventing aging, preventing adult diseases, promoting treatment for intractable diseases, suppressing carcinogenesis, and the like. Therefore, food and drink,
It can be advantageously used as cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４７】[0047]

【実施例Ｂ−８】〈生姜含有組成物〉生姜をミキサーに
かけて破砕し、この破砕物５重量部に対して、実施例Ａ
−２の方法で得た活性酸素消去能低減抑制剤を１重量部
混合溶解し、予備凍結の後、３日間凍結乾燥し、破砕機
で粉砕し活性酸素消去能が、１ｇ当たり約１２０単位の
粉末状生姜含有組成物を得た。本品を摂取することは、
生体にとって、生姜が本来有するビタミン、ミネラル、
食物繊維等の機能性成分に加えて、活性酸素消去能を強
化することとなり、健康の維持増進、老化防止、成人病
の予防、難病の治療促進、発癌の抑制等に好都合であ
る。従って、飲食物、化粧品、医薬品、それらの原材料
又は加工中間物等として有利に利用できる。Example B-8 <Ginger-containing composition> Ginger was crushed with a mixer, and 5 parts by weight of the crushed material was used in Example A.
1 part by weight of the active oxygen scavenging ability reduction inhibitor obtained by the method of -2, mixed and melted, preliminarily frozen, freeze-dried for 3 days, and pulverized with a crusher to have an active oxygen scavenging ability of about 120 units per gram. A powdered ginger-containing composition was obtained. Ingesting this product
For the living body, ginger originally has vitamins, minerals,
In addition to functional ingredients such as dietary fiber, the ability to scavenge active oxygen is strengthened, which is advantageous for maintaining and improving health, preventing aging, preventing adult diseases, promoting treatment of intractable diseases, suppressing carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４８】[0048]

【実施例Ｂ−９】〈青じそ含有組成物〉青じその葉をブ
ランチングした後、ミキサーにかけて破砕し、この破砕
物５重量部に対して、実施例Ａ−４の方法で得た活性酸
素消去能低減抑制剤を１重量部及びα−グルコシルルチ
ンを０．５重量部混合溶解し、次いで、脱水剤として無
水結晶トレハロースを４９重量部を混合し、室温に１日
放置し、粉砕機で粉砕し、活性酸素消去能が、１ｇ当た
り約２３０単位の粉末状青じそ含有組成物を得た。本品
は、青じそ本来の色調や風味をよく保っており、食欲を
そそるものであった。本品を摂取することは、生体にと
って、青じそが本来有するビタミン、ミネラル、食物繊
維等の機能性成分に加えて、活性酸素消去能を強化する
こととなり、健康の維持増進、老化防止、成人病の予
防、難病の治療促進、発癌の抑制等に好都合である。従
って、飲食物、化粧品、医薬品、それらの原材料又は加
工中間物等として有利に利用できる。[Example B-9] <Composition containing blue pea> After blanching the leaves of blue pea, they were crushed by a mixer, and 5 parts by weight of the crushed material was scavenged with active oxygen obtained by the method of Example A-4. 1 part by weight of an activity reduction inhibitor and 0.5 part by weight of α-glucosyl rutin were mixed and dissolved, and then 49 parts by weight of anhydrous crystalline trehalose was mixed as a dehydrating agent, left at room temperature for 1 day, and pulverized by a pulverizer. Then, a powdery blue dye-containing composition having an active oxygen scavenging ability of about 230 units per gram was obtained. This product kept the original color tone and flavor of blue vegetation well and was appetizing. Ingestion of this product will enhance the ability of living organisms to scavenge active oxygen, in addition to the functional components inherent in blue diso such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００４９】[0049]

【実施例Ｂ−１０】〈よもぎ含有組成物〉よもぎを、常
法に従って、適量の食塩を加えた沸騰水に入れてアク抜
きし、これをかるく脱水した後、破砕機にかけて破砕
し、この破砕物５重量部に実施例Ａ−５の方法で得た活
性酸素消去能低減抑制剤を１．３重量部及びαグルコシ
ルルチンを０．０５重量部混合し、５０℃で４時間通風
乾燥し、４０℃で１６時間真空乾燥し、粉砕して、活性
酸素消去能が、１ｇ当たり約７８０単位の粉末状よもぎ
含有組成物を得た。本品は、よもぎ本来の色調、風味を
よく保っており、食欲をそそるものであった。本品を摂
取することは、生体にとって、よもぎが本来有するビタ
ミン、ミネラル、食物繊維等の機能性成分に加えて、活
性酸素消去能を強化することとなり、健康の維持増進、
老化防止、成人病の予防、難病の治療促進、発癌の抑制
等に好都合である。従って、飲食物、化粧品、医薬品、
それらの原材料又は加工中間物等として有利に利用でき
る。Example B-10 <Wortwood-containing composition> A wormwood is put into boiling water to which an appropriate amount of salt has been added, and the water is drained off according to a conventional method. To 5 parts by weight of the product, 1.3 parts by weight of the active oxygen scavenging ability reduction inhibitor obtained by the method of Example A-5 and 0.05 part by weight of α-glucosyl rutin were mixed, and dried by ventilation at 50 ° C. for 4 hours, The resultant was vacuum-dried at 40 ° C. for 16 hours and pulverized to obtain a powdery wormwood-containing composition having an active oxygen scavenging ability of about 780 units per gram. This product kept the original color tone and flavor of wormwood well and was appetizing. Ingestion of this product will enhance the ability of living organisms to scavenge active oxygen, in addition to the functional ingredients such as vitamins, minerals, and dietary fiber that wormwood originally has, to maintain and improve health.
It is advantageous for preventing aging, preventing adult diseases, promoting treatment of intractable diseases, suppressing carcinogenesis, and the like. Therefore, food and drink, cosmetics, pharmaceuticals,
It can be advantageously used as such raw materials or processing intermediates.

【００５０】[0050]

【実施例Ｂ−１１】〈どくだみ含有組成物〉どくだみ乾
燥物（十薬、局方、大同製薬製）０．５重量部に１０重
量部の水を加え、加熱沸騰させ９０分間煎じた。バスケ
ット型遠心機で煎液を回収した。煎液を更に煮詰めて濃
度約５％液４重量部を得た。この煮詰め液２重量部に対
して実施例Ａ−４の方法で得た活性酸素消去能低減抑制
剤を０．５重量部を混合し、この溶液を常法により噴霧
乾燥し、活性酸素消去能が、１ｇ当たり約１４００単位
の粉末状どくだみ抽出物含有組成物を得た。本品はどく
だみ特有の嫌な臭いや味を低減していて、従来と比べて
口にしやすいものであった。本品を摂取することは、生
体にとって、どくだみが本来有するビタミン、ミネラ
ル、食物繊維等の機能性成分に加えて、活性酸素消去能
を強化することとなり、健康の維持増進、老化防止、成
人病の予防、難病の治療促進、発癌の抑制等に好都合で
ある。従って、飲食物、化粧品、医薬品、それらの原材
料又は加工中間物等として有利に利用できる。Example B-11 <Dokudami-containing composition> 10 parts by weight of water was added to 0.5 part by weight of a dried dokudami product (Juyaku, Pharmacopoeia, manufactured by Daido Pharmaceutical Co., Ltd.), heated to boiling, and decocted for 90 minutes. The decoction was collected with a basket-type centrifuge. The decoction was further boiled to obtain 4 parts by weight of a liquid having a concentration of about 5%. 0.5 parts by weight of the active oxygen scavenging ability reducing inhibitor obtained by the method of Example A-4 was mixed with 2 parts by weight of the boiled liquid, and this solution was spray-dried by a conventional method to obtain an active oxygen scavenging ability. Obtained about 1400 units of a powdery dust extract containing composition per gram. This product reduced the unpleasant odor and taste peculiar to KUDOMI and was easier to eat than conventional ones. Ingestion of this product will enhance the ability of active organisms to scavenge active oxygen in addition to the functional components inherent in the body, such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００５１】[0051]

【実施例Ｂ−１２】〈アロエ含有組成物〉アロエをミキ
サーにかけて破砕し、この破砕物１０重量部に対して、
実施例Ａ−１の方法で得た活性酸素消去能低減抑制剤を
１重量部及び茶抽出物０．１重量部を７０℃で２日間通
風乾燥し、粉砕機で粉砕し、活性酸素消去能が、１ｇ当
たり約５８００単位の粉末状アロエ含有組成物を得た。
本品は、アロエの苦味が低減し、従来品に比べて口にし
やすいものであった。本品を摂取することは、生体にと
って、アロエが本来有するビタミン、ミネラル、食物繊
維等の機能性成分に加えて、活性酸素消去能を強化する
こととなり、健康の維持増進、老化防止、成人病の予
防、難病の治療促進、発癌の抑制等に好都合である。従
って、飲食物、化粧品、医薬品、それらの原材料又は加
工中間物等として有利に利用できる。Example B-12 <Aloe-containing composition> Aloe was crushed with a mixer, and 10 parts by weight of the crushed material was used.
1 part by weight of the active oxygen scavenging ability reduction inhibitor obtained by the method of Example A-1 and 0.1 part by weight of a tea extract were air-dried at 70 ° C. for 2 days, pulverized with a pulverizer, and then subjected to active oxygen scavenging ability. Obtained about 5800 units of the powdered aloe-containing composition per gram.
This product reduced the bitterness of aloe and was easier to eat than conventional products. Ingestion of this product will enhance the ability of active organisms to scavenge active oxygen in addition to the functional ingredients inherent in aloe, such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００５２】[0052]

【実施例Ｂ−１３】〈白菜浅漬含有組成物〉白菜浅漬を
細断機にかけ、この細断物１０重量部に対して、実施例
Ａ−１の方法で得た活性酸素消去能低減抑制剤を１重量
部混合溶解し、５５℃で１６時間通風乾燥し、活性酸素
消去能が１ｇ当り約５４００単位の細片状の白菜浅漬含
有組成物を得た。本品は、白菜浅漬の色調をよく保って
おり、口に含むとその漬物の風味が広がり食欲をそそる
ものであった。本品を摂取することは、生体にとって、
白菜浅漬が本来有するビタミン、ミネラル、食物繊維等
の機能性成分に加えて、活性酸素消去能を強化すること
となり、健康の維持増進、老化防止、成人病の予防、難
病の治療促進、発癌の抑制等に好都合である。従って、
飲食物、化粧品、医薬品、それらの原材料又は加工中間
物等として有利に利用できる。従って、ふりかけ、おに
ぎりの素、ラーメンスープの具等の飲食物のみならず、
健康食品、その他化粧品、医薬品、それらの原料又は中
間物等として有利に利用できる。Example B-13 <Contained composition containing lightly pickled Chinese cabbage> The lightly pickled Chinese cabbage was shredded, and 10 parts by weight of the shredded material was used to suppress the active oxygen scavenging ability obtained by the method of Example A-1. Was dissolved in a mixture of 1 part by weight and dried by ventilation at 55 ° C. for 16 hours to obtain a strip-shaped composition containing lightly pickled Chinese cabbage having an active oxygen elimination capacity of about 5400 units per gram. This product kept the color of lightly pickled Chinese cabbage well, and when included in the mouth, the flavor of the pickles was widened and the appetite was apprehended. Ingestion of this product is
In addition to the functional components such as vitamins, minerals, and dietary fiber inherent in Chinese cabbage lightly pickled, it will enhance the ability to eliminate active oxygen, maintain and improve health, prevent aging, prevent adult diseases, promote the treatment of intractable diseases, promote carcinogenesis. This is convenient for suppression and the like. Therefore,
It can be advantageously used as food and drink, cosmetics, pharmaceuticals, their raw materials or processed intermediates, and the like. Therefore, not only foods such as sprinkles, rice balls, ramen soup ingredients,
It can be advantageously used as health foods, other cosmetics, pharmaceuticals, raw materials or intermediates thereof.

【００５３】[0053]

【実施例Ｂ−１４】〈野沢菜漬含有組成物〉野沢菜漬を
細断機にかけ、この細断物１０重量部に対して、実施例
Ａ−１の方法で得た活性酸素消去能低減抑制剤を１重量
部混合溶解し、５５℃で１６時間通風乾燥し、活性酸素
消去能が１ｇ当り約２６００単位の細片状の野沢菜漬含
有組成物を得た。本品は、野沢菜漬の色調をよく保って
おり、口に含むとその漬物の風味が広がり、食欲をそそ
るものであった。本品を摂取することは、生体にとっ
て、野沢菜漬が本来有するビタミン、ミネラル、食物繊
維等の機能性成分に加えて、活性酸素消去能を強化する
こととなり、健康の維持増進、老化防止、成人病の予
防、難病の治療促進、発癌の抑制等に好都合である。従
って、飲食物、化粧品、医薬品、それらの原材料又は加
工中間物等として有利に利用できる。Example B-14 <Nozawanazuke-containing composition> A Nozawanazuke-containing composition was shredded, and 10 parts by weight of the shredded product was reduced in active oxygen scavenging ability obtained by the method of Example A-1. One part by weight of the inhibitor was mixed and dissolved, and air-dried at 55 ° C. for 16 hours to obtain a strip-shaped Nozawanazuke-containing composition having an active oxygen eliminating ability of about 2600 units per gram. This product kept the color of Nozawana pickles well, and when included in the mouth, the flavor of the pickles spread and was appetizing. Ingestion of this product, for the living body, in addition to functional ingredients such as vitamins, minerals, dietary fiber, etc. inherent to Nozawanazuke, enhances the ability to scavenge active oxygen, maintain and improve health, prevent aging, It is advantageous for prevention of adult diseases, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００５４】[0054]

【実施例Ｂ−１５】〈白菜キムチ含有組成物〉白菜キム
チを細断機にかけ、この細断物１０重量部に対して、実
施例Ａ−４の方法で得た活性酸素消去能低減抑制剤を１
重量部混合溶解し、５５℃で１６時間通風乾燥し、活性
酸素消去能が１ｇ当り約１６００単位の細片状の白菜キ
ムチ含有組成物を得た。本品は、白菜キムチの色調をよ
く保っており、口に含むとその漬物の風味が広がり、食
欲をそそるものであった。本品を摂取することは、生体
にとって、白菜キムチが本来有するビタミン、ミネラ
ル、食物繊維等の機能性成分に加えて、活性酸素消去能
を強化することとなり、健康の維持増進、老化防止、成
人病の予防、難病の治療促進、発癌の抑制等に好都合で
ある。従って、飲食物、化粧品、医薬品、それらの原材
料又は加工中間物等として有利に利用できる。Example B-15 <Chinese cabbage kimchi-containing composition> A Chinese cabbage kimchi was shredded into shredders and 10 parts by weight of the shredded material was used to suppress the active oxygen scavenging ability obtained by the method of Example A-4. 1
Parts by weight were mixed and dissolved, and dried by ventilation at 55 ° C. for 16 hours to obtain a stripy Chinese cabbage kimchi-containing composition having an active oxygen eliminating ability of about 1600 units per gram. This product kept the color tone of Chinese cabbage kimchi well, and when included in the mouth, the flavor of the pickles spread and was appetizing. Ingestion of this product will enhance the ability of active organisms to scavenge active oxygen in addition to the functional ingredients inherent in Chinese cabbage kimchi, such as vitamins, minerals, and dietary fiber. It is advantageous for prevention of diseases, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００５５】[0055]

【実施例Ｂ−１６】〈大豆含有組成物〉大豆を５倍容の
水に室温で一夜浸漬し、これを水切りした大豆１０重量
部を釜にとり、これに実施例Ａ−１の方法で得た活性酸
素消去能低減抑制剤３５重量部及び水３５重量部を加
え、中火で煮沸し、ブリックス計で濃度約７０％まで煮
詰めて煮豆とし、これをざるに上げ、４０℃で一夜通風
乾燥し、活性酸素消去能が１ｇ当り約２５単位の大豆含
有組成物を得た。本品は、大豆煮豆の色調をよく保って
おり、口に含むと大豆の風味が広がり食欲をそそるもの
であった。また、本品は、大豆組織内にトレハロース含
水結晶の晶出が見られ、該組織を良く維持し、口当たり
も良好で、歯ごたえのある感触を楽しむことのできる固
状食品であった。本品を摂取することは、生体にとっ
て、大豆が本来有するビタミン、ミネラル、食物繊維等
の機能性成分に加えて、活性酸素消去能を強化すること
となり、健康の維持増進、老化防止、成人病の予防、難
病の治療促進、発癌の抑制等に好都合である。従って、
飲食物、化粧品、医薬品、それらの原材料又は加工中間
物等として有利に利用できる。Example B-16 <Soybean-containing composition> Soybeans were immersed in 5 volumes of water at room temperature overnight, and 10 parts by weight of soybeans were drained and placed in a kettle, and obtained by the method of Example A-1. Add 35 parts by weight of the active oxygen scavenging inhibitor and 35 parts by weight of water, boil over medium heat, boil down to a concentration of about 70% with a Brix meter, boil the beans, raise it to a zigzag, and air-dry overnight at 40 ° C. Then, a soybean-containing composition having an active oxygen scavenging ability of about 25 units per gram was obtained. This product kept the color of boiled soybeans well, and when included in the mouth, the flavor of soybeans widened and the appetite was aroused. In addition, this product was a solid food product in which hydrated trehalose crystals were found in the soybean tissue, the texture was well maintained, the palatability was good, and the chewy feel was enjoyed. Ingestion of this product will enhance the ability of living organisms to scavenge active oxygen in addition to the functional components inherent in soybeans, such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore,
It can be advantageously used as food and drink, cosmetics, pharmaceuticals, their raw materials or processed intermediates, and the like.

【００５６】[0056]

【実施例Ｂ−１７】〈りんご含有組成物〉りんごの皮を
むき、スライサーにかけて得た厚さ約５ｍｍのりんご切
断物を、実施例Ａ−１の方法で得た活性酸素消去能低減
抑制剤を水に温度約８０℃で加熱溶解した濃度約７５％
溶液に３０分間浸漬し、これをざるに上げ、３５℃で一
夜通風乾燥し、活性酸素消去能が１ｇ当り約５０単位の
りんご含有組成物を得た。本品は、りんごの色調をよく
保っており、口に含むとりんごの風味が広がり食欲をそ
そるものであった。また、本品は、りんご組織内にトレ
ハロース含水結晶の晶出が見られ、該組織をよく維持
し、口当たりも良好で、歯ごたえのある感触を楽しむこ
とのできる固状食品であった。本品を摂取することは、
生体にとって、りんごが本来有するビタミン、ミネラ
ル、食物繊維等の機能性成分に加えて、活性酸素消去能
を強化することとなり、健康の維持増進、老化防止、成
人病の予防、難病の治療促進、発癌の抑制等に好都合で
ある。従って、飲食物、化粧品、医薬品、それらの原材
料又は加工中間物等として有利に利用できる。Example B-17 <Apple-containing composition> An apple having a thickness of about 5 mm obtained by peeling an apple and slicing the sliced apple was used to obtain an active oxygen scavenging ability reducing inhibitor obtained by the method of Example A-1. About 75% dissolved in water at a temperature of about 80 ° C
The resultant was immersed in the solution for 30 minutes, raised to a bristle, and air-dried at 35 ° C. overnight to obtain an apple-containing composition having an active oxygen scavenging ability of about 50 units per gram. This product kept the color tone of the apple well, and when included in the mouth, the flavor of the apple spread and was appetizing. In addition, this product was a solid food product in which hydrates of trehalose-containing crystals were observed in the apple tissue, the texture was well maintained, the palatability was good, and the chewy feel was enjoyed. Ingesting this product
For the living body, in addition to functional components such as vitamins, minerals, and dietary fiber that apples originally have, it will enhance the ability to eliminate active oxygen, maintain and improve health, prevent aging, prevent adult diseases, promote the treatment of intractable diseases, It is convenient for suppressing carcinogenesis. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００５７】[0057]

【実施例Ｂ−１８】〈人参含有組成物〉人参をスライサ
ーにかけてスライスしこのスライス５重量部を、水６０
重量部に実施例Ａ−１の方法で得た活性酸素消去能低減
抑制剤１４０重量部を加熱溶解しわずかに煮沸している
温度約１０５℃のシラップ（濃度約７３％）に投入し、
その温度が約１１０℃になるまで約２０分間煮沸濃縮を
続けてフライ様に加熱脱水処理を行い、次いでこれをざ
るに上げ、５０℃で５時間通風乾燥し活性酸素消去能が
１ｇ当り約２００単位の細片状人参含有組成物を得た。
本品は、表面にトレハロース含水結晶の付着した人参の
揚物風製品で、人参の色調をよく保っており、食欲をそ
そるものであった。また、本品は吸湿性が低く、保存安
定性に優れ、取扱い容易であった。本品を摂取すること
は、生体にとって、人参が本来有するビタミン、ミネラ
ル、食物繊維等の機能性成分に加えて、活性酸素消去能
を強化することとなり、健康の維持増進、老化防止、成
人病の予防、難病の治療促進、発癌の抑制等に好都合で
ある。従って、飲食物、化粧品、医薬品、それらの原材
料又は加工中間物等として有利に利用できる。Example B-18 <Ginseng-containing composition> Ginseng was sliced by slicing, and 5 parts by weight of this slice was added to 60 parts of water.
140 parts by weight of the active oxygen scavenging ability-reducing inhibitor obtained by the method of Example A-1 was dissolved by heating and added to a slightly boiled syrup (concentration of about 73%) at a temperature of about 105 ° C.
The mixture was boiled and concentrated for about 20 minutes until the temperature reached about 110 ° C., and then heated and dehydrated in a fry-like manner. A unit of ginseng-containing composition was obtained.
This product was a deep-fried ginseng-like product with trehalose hydrated crystals attached to its surface, which kept the ginseng color well and was appetizing. The product had low hygroscopicity, excellent storage stability, and was easy to handle. Ingestion of this product will enhance the ability of living organisms to scavenge active oxygen in addition to the functional components inherent in ginseng, such as vitamins, minerals, and dietary fiber. It is convenient for prevention of cancer, promotion of treatment for intractable diseases, suppression of carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００５８】[0058]

【実施例Ｂ−１９】〈りんご含有組成物〉りんごの皮を
むきスライサーにかけて得た厚さ約５ｍｍのりんご切断
物４重量部を、水６０重量部に実施例Ａ−１の方法で得
た活性酸素消去能低減抑制剤１４０重量部を加熱溶解し
煮沸している温度約１１０℃のシラップ（濃度約８０
％）に投入し、その温度が約１１４℃になるまで約１０
分間煮沸濃縮を続けてフライ様に加熱脱水処理を行い、
次いでこれをざるに上げ、３５℃で一夜通風乾燥し、活
性酸素消去能が１ｇ当り約３０単位のりんご含有組成物
を得た。本品は、表面にトレハロース含水結晶の付着し
たりんごの揚物風製品で、りんごの色調をよく保ってお
り、口に含むとりんごの風味が広がり食欲をそそるもの
であった。また、本品は、吸湿性が低く、保存安定性に
優れ、取扱い容易であった。本品を摂取することは、生
体にとって、りんごが本来有するビタミン、ミネラル、
食物繊維等の機能性成分に加えて、活性酸素消去能を強
化することとなり、健康の維持増進、老化防止、成人病
の予防、難病の治療促進、発癌の抑制等に好都合であ
る。従って、飲食物、化粧品、医薬品、それらの原材料
又は加工中間物等として有利に利用できる。Example B-19 <Apple-containing composition> 4 parts by weight of a cut apple having a thickness of about 5 mm obtained by peeling an apple and slicing it was obtained by the method of Example A-1 in 60 parts by weight of water. A syrup at a temperature of about 110 ° C. (concentration of about 80
%) Until the temperature reaches about 114 ° C.
Continue boiling and concentrating for a minute, heat-dehydrate like a fry,
Then, the mixture was removed and dried at 35 ° C. overnight with ventilation to obtain an apple-containing composition having an active oxygen eliminating ability of about 30 units per gram. This product was a deep-fried apple-like product with trehalose hydrated crystals attached to the surface, and the color tone of the apple was well maintained. The product had low hygroscopicity, excellent storage stability, and was easy to handle. Ingestion of this product is important for the living body, because vitamins, minerals,
In addition to functional ingredients such as dietary fiber, the ability to scavenge active oxygen is strengthened, which is advantageous for maintaining and improving health, preventing aging, preventing adult diseases, promoting treatment of intractable diseases, suppressing carcinogenesis, and the like. Therefore, they can be advantageously used as foods and drinks, cosmetics, pharmaceuticals, their raw materials or processed intermediates.

【００５９】[0059]

【実施例Ｂ−２０】〈チューインガム〉ガムベース３重
量部を柔らかくなる程度に加熱溶解し、これにトレハロ
ース含水結晶６重量部、及び実施例Ｂ−９の方法で得た
青じそ含有組成物１重量部及び茶抽出物０．０１重量部
とを加え、更に適量の香料と着色料とを混合し、常法に
従って、ロールにより練り合わせ、成形、包装して活性
酸素消去能が、１ｇ当たり約１２０単位の製品を得た。
本品は、テクスチャー、風味とも良好なチューインガム
である。また、本品は、使われている糖質がトレハロー
スであることから、虫歯誘発菌に利用されにくく、虫歯
になりにくい特徴も有している。[Example B-20] <Chewing gum> 3 parts by weight of a gum base were dissolved by heating to a degree of softening, and 6 parts by weight of trehalose hydrated crystal and 1 part by weight of a blue dye containing composition obtained by the method of Example B-9. And 0.01 part by weight of a tea extract, and further, an appropriate amount of a fragrance and a colorant are mixed, and the mixture is kneaded with a roll according to a conventional method, molded and packaged to have an active oxygen eliminating ability of about 120 units per gram. Got the product.
This product is a chewing gum with good texture and flavor. In addition, this product has a feature that it is hard to be used for caries-inducing bacteria and hardly causes dental caries because the saccharide used is trehalose.

【００６０】[0060]

【実施例Ｂ−２１】〈だんご〉モチ種澱粉１０重量部に
水１２重量部を混合し、加熱糊化しつつ、これに実施例
Ｂ−１０の方法で得た、よもぎ含有組成物０．５重量部
を混和し、以後、常法に従って、成形、包装して活性酸
素消去能が、１ｇ当たり約１２単位のだんごを製造し
た。本品は、よもぎの色調、風味に優れ、口当たりも良
好なよもぎだんごである。Example B-21 <Dango> 10 parts by weight of waxy seed starch was mixed with 12 parts by weight of water, and gelatinized by heating, and the mixture was mixed with 0.5 g of wormwood-containing composition obtained by the method of Example B-10. The resulting mixture was mixed in parts by weight, and then molded and packaged according to a conventional method to produce a dumpling having an active oxygen scavenging ability of about 12 units per gram. This product is a wormwood dumpling with excellent color and flavor of wormwood and good mouthfeel.

【００６１】[0061]

【実施例Ｂ−２２】〈栄養剤〉結晶性α−マルトース２
０重量部、グリシン１．１重量部、グルタミン酸ナトリ
ウム０．１８重量部、食塩１．２重量部、クエン酸１重
量部、乳酸カルシウム０．４重量部、実施例Ｂ−１の方
法で得たほうれん草含有組成物０．３重量部、チアミン
０．０１重量部及びリボフラビン０．０１重量部からな
る配合物を調製する。この配合物２４ｇずつをラミネー
トアルミ製小袋に充填し、ヒートシールして活性酸素消
去能が、１ｇ当たり、約１２単位の経管栄養剤を調製し
た。本栄養剤は、一袋を約３００乃至５００ｍＬの水に
溶解し、経口的摂取することも、また経管方法により鼻
腔、胃、腸等への非経口的栄養補給液としても有利に利
用できる。Example B-22 <Nutrient> Crystalline α-maltose 2
0 parts by weight, 1.1 parts by weight of glycine, 0.18 parts by weight of sodium glutamate, 1.2 parts by weight of sodium chloride, 1 part by weight of citric acid, 0.4 parts by weight of calcium lactate, obtained by the method of Example B-1 A formulation comprising 0.3 parts by weight of a spinach-containing composition, 0.01 parts by weight of thiamine and 0.01 parts by weight of riboflavin is prepared. Each 24 g of this compound was filled into a laminated aluminum pouch, and heat-sealed to prepare a tube nutrient having an active oxygen eliminating ability of about 12 units per 1 g. This nutritional supplement can be used advantageously by dissolving one bag in about 300 to 500 mL of water and ingesting it orally, or as a parenteral nutritional supplement for the nasal cavity, stomach, intestine, etc. by tube administration. .

【００６２】[0062]

【実施例Ｂ−２３】〈化粧用クリーム〉モノステアリン
酸ポリオキシエチレングリコール２重量部、自己乳化型
モノステアリン酸グリセリン５重量部、実施例Ｂ−１２
の方法で得たアロエ含有組成物２．８重量部、α−グル
コシルルチン０．２重量部、流動パラフィン１重量部、
トリオクタン酸グリセリル１０重量部及び防腐剤の適量
を、常法に従って加熱溶解し、これにＬ−乳酸２重量
部、１，３−ブチレングリコール５重量部及び精製水６
６重量部を加え、ホモゲナイザーにかけ乳化し、更に香
料の適量を加えて攪拌混合して、活性酸素消去能が、１
ｇ当たり、約４６単位の化粧用クリームを製造した。本
品は、日焼けによる火傷肌の治療、日焼けの予防、美肌
剤、色白剤等として、更には、しみ、そばかす、あざ、
しわ等皮膚の老化現象の発生抑制剤等として有利に利用
できる。Example B-23 <Cosmetic cream> 2 parts by weight of polyoxyethylene glycol monostearate, 5 parts by weight of self-emulsifying glyceryl monostearate, Example B-12
2.8 parts by weight of the aloe-containing composition obtained by the method described above, 0.2 parts by weight of α-glucosylrutin, 1 part by weight of liquid paraffin,
10 parts by weight of glyceryl trioctanoate and an appropriate amount of a preservative were dissolved by heating according to a conventional method, and 2 parts by weight of L-lactic acid, 5 parts by weight of 1,3-butylene glycol and 6 parts of purified water
6 parts by weight, emulsified with a homogenizer, and then added with an appropriate amount of a fragrance and mixed with stirring.
About 46 units of cosmetic cream were produced per gram. This product is used as a treatment for burn skin due to sunburn, prevention of sunburn, skin beautifying agent, skin lightening agent, etc., as well as spots, freckles, bruises,
It can be advantageously used as an agent for suppressing the occurrence of skin aging such as wrinkles.

【００６３】[0063]

【実施例Ｂ−２４】〈浴用剤〉ＤＬ−乳酸ナトリウム２
１重量部、ピルビン酸ナトリウム８重量部、実施例Ｂ−
７の方法で得た柚含有組成物５重量部、α−グルコシル
ルチン１重量部及びエタノール４０重量部を、精製水２
６重量部及び着色料、香料の適量と混合し、活性酸素消
去能が、１ｇ当たり約２２０単位の浴用剤を製造した。
本品は、美肌剤、色白剤として好適であり、入浴用の湯
に１００乃至１０，０００倍に希釈して利用すればよ
い。本品は、入浴用の湯の場合と同様に、洗顔用水、化
粧水等に希釈して利用することも有利に実施できる。Example B-24 <Bath agent> DL-sodium lactate 2
1 part by weight, sodium pyruvate 8 parts by weight, Example B-
5 parts by weight of the citrus-containing composition, 1 part by weight of α-glucosylrutin and 40 parts by weight of ethanol
By mixing with 6 parts by weight and an appropriate amount of a coloring agent and a fragrance, a bath agent having an active oxygen scavenging ability of about 220 units per gram was produced.
The product is suitable as a skin-beautifying agent and a skin-whitening agent, and may be used by diluting it in hot water for bathing 100 to 10,000 times. As in the case of hot water for bathing, the product can be advantageously used by diluting it in face wash water, lotion and the like.

【００６４】[0064]

【実施例Ｂ−２５】〈軟膏〉酢酸ナトリウム・三水塩１
重量部、ＤＬ−乳酸カルシウム４重量部をグリセリン１
０重量部と均一に混合し、この混合物を、ワセリン５０
重量部、木ロウ１０重量部、ラノリン５重量部、ゴマ油
１４．５重量部、実施例Ｂ−１１の方法で得たどくだみ
含有組成物６重量部及びはっか油０．５重量部の混合物
に加えて、更に均一に混和して活性酸素消去能が、１ｇ
当たり約５４単位の軟膏を製造した。本品は、化膿止
め、美肌剤、色白剤等として、更には外傷、火傷の治療
促進剤等として有利に利用できる。Example B-25 <Ointment> Sodium acetate trihydrate 1
Parts by weight, 4 parts by weight of DL-calcium lactate in glycerin 1
0 parts by weight, and the mixture is mixed with Vaseline 50
Parts by weight, 10 parts by weight of wood wax, 5 parts by weight of lanolin, 14.5 parts by weight of sesame oil, a mixture of 6 parts by weight of the dokudami-containing composition obtained by the method of Example B-11 and 0.5 part by weight of pepper oil. And evenly mixed to make the active oxygen scavenging ability 1g
About 54 units of ointment were produced per unit. The product can be advantageously used as a purulent, a skin-beautifying agent, a skin-whitening agent and the like, as well as a therapeutic accelerator for trauma and burns.

【００６５】[0065]

【実施例Ｂ−２６】〈錠剤〉アスコルビン酸２０重量部
に結晶性β−マルトース１３重量部、コーンスターチ４
重量部および実験５の方法で得た本発明のトレハロース
を使用した、おおばこ含有組成物３重量部を均一に混合
した後、直径１２ｍｍ、２０Ｒ杵を用いて、打錠し錠剤
を得た。本品は、１ｇ当たり約５６０単位の活性酸素消
去能を有していた。本品は、健康の維持増進のため、大
人一日当たり１乃至１０錠経口摂取すればよい。とりわ
け、健胃、整腸、解熱等の目的で使用できる。Example B-26 <Tablet> 20 parts by weight of ascorbic acid, 13 parts by weight of crystalline β-maltose, corn starch 4
3 parts by weight of the stomach-containing composition using the trehalose of the present invention obtained by the method of Experiment 5 and the trehalose obtained by the method of Experiment 5 were uniformly mixed, and then tableted using a 20 mm punch with a diameter of 12 mm to obtain tablets. This product had an active oxygen scavenging ability of about 560 units per gram. This product may be taken orally in an amount of 1 to 10 tablets per adult per day to maintain and promote health. In particular, it can be used for purposes such as stomachic, intestinal control, and antipyretic.

【００６６】[0066]

【発明の効果】上記したように、本発明は、トレハロー
スを有効成分とする活性酸素消去能低減抑制剤と、トレ
ハロース又は該低減抑制剤を含有せしめることを特徴と
する活性酸素消去能の低減抑制方法を提供するものであ
り、併せて該方法により活性酸素消去能の低減を抑制し
た植物性可食物及び／又は植物性抗酸化物質含有組成物
を提供するものである。本組成物の摂取は、生体にとっ
ては、植物性可食物等のもつビタミン、ミネラル、食物
繊維等の機能性成分に加えて、新たに活性酸素消去能の
低減が少なく、より安定した活性酸素消去能を容易に強
化、補給できることとなり、健康の維持増進、老化防
止、成人病の予防、難病の治療促進、発癌の抑制等に大
きく寄与できることになる。従って、本発明の確立は、
植物性可食物等の加工、利用分野に第四の機能性成分と
も言うべき新たな健康資源を開拓することとなり、これ
が与える影響は広く、とりわけ、飲食物、化粧品、医薬
品等の産業界に与える工業的意義はきわめて高い。As described above, the present invention provides an active oxygen scavenging ability reduction inhibitor containing trehalose as an active ingredient, and a reduction in active oxygen scavenging ability characterized by containing trehalose or the reduction inhibitor. Another object of the present invention is to provide a plant edible food and / or a plant antioxidant-containing composition in which the reduction of the ability to scavenge active oxygen is suppressed by the method. For the living body, in addition to vitamins, minerals, and functional components such as dietary fiber possessed by plant edible foods, the reduction of the active oxygen scavenging ability is less, and the active oxygen scavenging is more stable for the living body. It can easily reinforce and replenish the ability, and can greatly contribute to maintenance and promotion of health, prevention of aging, prevention of adult diseases, promotion of treatment of intractable diseases, suppression of carcinogenesis, and the like. Therefore, the establishment of the present invention
It will open up new health resources that can be called the fourth functional ingredient in the processing and utilization fields of plant edible foods, etc., and this will have a wide impact, especially on the food, beverage, cosmetics, pharmaceutical and other industries. The industrial significance is extremely high.

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.⁶ 識別記号 ＦＩ Ａ６１Ｋ 31/715 ＡＧＺ Ａ６１Ｋ 31/715 ＡＧＺ Ｃ０９Ｋ 15/06 Ｃ０９Ｋ 15/06 15/34 15/34 // Ａ６１Ｋ 35/78 Ａ６１Ｋ 35/78 Ｃ ──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 31/715 AGZ A61K 31/715 AGZ C09K 15/06 C09K 15/06 15/34 15/34 // A61K 35/78 A61K 35 / 78 C

Claims (26)

【特許請求の範囲】[Claims] 【請求項１】 トレハロースを有効成分とする活性酸素
消去能低減抑制剤。An active oxygen scavenging ability reduction inhibitor comprising trehalose as an active ingredient. 【請求項２】 トレハロース以外の糖質を含む請求項１
記載の活性酸素消去能低減抑制剤。2. The method according to claim 1, which comprises a saccharide other than trehalose.
The active oxygen scavenging ability reduction inhibitor according to the above. 【請求項３】 プルラン及び／又はシクロデキストリン
を含む請求項１又は２記載の活性酸素消去能低減抑制
剤。3. The inhibitor for reducing active oxygen scavenging ability according to claim 1, comprising pullulan and / or cyclodextrin. 【請求項４】 トレハロースを無水物換算で約２０ｗ／
ｗ％以上含有する請求項１、２又は３記載の活性酸素消
去能低減抑制剤。4. An amount of trehalose in an amount of about 20 w /
The active oxygen scavenging ability reduction inhibitor according to claim 1, which contains at least w%. 【請求項５】 植物由来の活性酸素消去能を有する物質
に、トレハロース、又は、請求項１、２、３又は４記載
の活性酸素消去能低減抑制剤を水性媒体で含有せしめる
ことを特徴とする活性酸素消去能の低減抑制方法。5. A plant-derived substance having the ability to scavenge active oxygen, wherein trehalose or the active oxygen scavenging ability reduction inhibitor according to claim 1, 2, 3, or 4 is contained in an aqueous medium. A method for suppressing reduction of active oxygen scavenging ability. 【請求項６】 植物由来の活性酸素消去能を有する物質
が、植物性可食物及び／又は植物性抗酸化物質である請
求項５記載の活性酸素消去能の低減抑制方法。6. The method according to claim 5, wherein the plant-derived substance having an ability to scavenge active oxygen is a plant edible food and / or a plant antioxidant. 【請求項７】 植物性可食物が、可食性植物の器官及び
／又は組織、又はそれら可食部の切断物、破砕物、粉砕
物、乾燥物、漬物及び／又は抽出物である請求項６記載
の活性酸素消去能の低減抑制方法。7. The plant edible food is an organ and / or tissue of an edible plant, or a cut, crushed, crushed, dried, pickled and / or an extract of those edible parts. The method for suppressing reduction of active oxygen scavenging ability according to the above. 【請求項８】 植物性抗酸化物質が、酵素、色素、ポリ
フェノール及びビタミンから選ばれる植物由来の抗酸化
物質である請求項６記載の活性酸素消去能の低減抑制方
法。8. The method according to claim 6, wherein the plant antioxidant is a plant-derived antioxidant selected from enzymes, pigments, polyphenols and vitamins. 【請求項９】 有効成分としてのトレハロースを、植物
由来の活性酸素消去能を有する物質に対して、無水物換
算で約１ｗ／ｗ％以上含有せしめる請求項５、６、７又
は８記載の活性酸素消去能の低減抑制方法。9. The activity according to claim 5, wherein the trehalose as an active ingredient is contained in an amount of about 1% w / w or more in terms of anhydride relative to a plant-derived substance having an ability to scavenge active oxygen. A method for suppressing the reduction of oxygen scavenging ability. 【請求項１０】 殺菌及び／又は乾燥工程を含む請求項
５、６、７、８又は９記載の活性酸素消去能の低減抑制
方法。10. The method according to claim 5, further comprising a sterilization and / or drying step. 【請求項１１】 植物由来の活性酸素消去能を有する物
質に、トレハロース、又は請求項１、２、３又は４記載
の活性酸素消去能低減抑制剤を含有せしめ、活性酸素消
去能の低減を抑制した組成物。11. A plant-derived substance having an ability to scavenge active oxygen, which contains trehalose or the inhibitor for reducing the ability to scavenge active oxygen according to claim 1, 2, or 4 to suppress the decrease in the ability to scavenge active oxygen. Composition. 【請求項１２】 植物由来の活性酸素消去能を有する物
質が、植物性可食物及び／又は植物性抗酸化物質である
請求項１１記載の組成物。12. The composition according to claim 11, wherein the plant-derived substance having an ability to scavenge active oxygen is a plant edible food and / or a plant antioxidant. 【請求項１３】 植物性可食物が、可食性植物の器官及
び／又は組織、又はそれら可食部の切断物、破砕物、粉
砕物、乾燥物、漬物及び／又は抽出物である請求項１２
記載の組成物。13. The edible plant food is an organ and / or tissue of an edible plant, or a cut, crushed, crushed, dried, pickled, and / or extracted extract of the edible part.
A composition as described. 【請求項１４】 植物性抗酸化物質が、酵素、色素、ポ
リフェノール及びビタミンから選ばれる植物由来の抗酸
化物質である請求項１２記載の組成物。14. The composition according to claim 12, wherein the plant antioxidant is a plant-derived antioxidant selected from enzymes, pigments, polyphenols and vitamins. 【請求項１５】 有効成分としてのトレハロースの含量
が、無水物換算で約１ｗ／ｗ％以上である請求項１１、
１２、１３又は１４記載の組成物。15. The method according to claim 11, wherein the content of trehalose as an active ingredient is about 1% w / w or more in terms of anhydride.
15. The composition according to 12, 13, or 14. 【請求項１６】 トレハロースを、非晶質状態で含有し
ているか、又は含水結晶状態で含有している請求項１
１、１２、１３、１４又は１５記載の組成物。16. The trehalose is contained in an amorphous state or in a hydrated crystalline state.
16. The composition according to 1, 12, 13, 14 or 15. 【請求項１７】 必須ミネラル、食物繊維、ビフィズス
菌増殖促進糖質、ビタミン及び生理活性物質から選ばれ
る１種又は２種以上を含有せしめた請求項１１、１２、
１３、１４、１５又は１６記載の組成物。17. The method according to claim 11, wherein one or two or more selected from essential minerals, dietary fiber, bifidobacteria growth promoting carbohydrates, vitamins and physiologically active substances are contained.
17. The composition according to 13, 14, 15 or 16. 【請求項１８】 殺菌及び／又は乾燥工程を施された請
求項１１、１２、１３、１４、１５、１６又は１７記載
の組成物。18. The composition according to claim 11, which has been subjected to a sterilization and / or drying step. 【請求項１９】 ニトロブルーテトラゾリウム法で測定
された活性酸素消去能を、１ｇ当たり５単位以上有して
いる請求項１１、１２、１３、１４、１５、１６、１７
又は１８記載の組成物。19. The method of claim 11, 12, 13, 14, 15, 16, 17 having an active oxygen scavenging ability of 5 units or more per gram as measured by the nitroblue tetrazolium method.
Or the composition according to 18. 【請求項２０】 組成物が、飲食物、化粧品、医薬品、
又はこれらの原材料もしくは中間加工物である請求項１
１、１２、１３、１４、１５、１６、１７、１８又は１
９記載の組成物。20. The composition according to claim 1, wherein the composition is a food or drink, a cosmetic, a pharmaceutical,
Or a raw material or an intermediate product thereof.
1, 12, 13, 14, 15, 16, 17, 18 or 1
9. The composition according to 9. 【請求項２１】 請求項１１、１２、１３、１４、１
５、１６、１７、１８、１９又は２０記載の組成物を含
有せしめた飲食物、化粧品又は医薬品。21. The method of claim 11, 12, 13, 14, 1,
A food, drink, cosmetic or pharmaceutical containing the composition according to 5, 16, 17, 18, 19 or 20. 【請求項２２】 可食性植物の組織に、トレハロースを
無水物換算で約３０ｗ／ｗ％以上含有せしめた組成物。22. A composition comprising edible plant tissue containing trehalose in an amount of about 30% w / w or more in terms of anhydride. 【請求項２３】 可食性植物の組織が、可食性植物の器
官、組織、それら可食物の切断物及び漬物から選ばれる
組織であって、その組織の内部に、トレハロースを非晶
質状態で含有しているか、又は含水結晶状態で含有して
いる請求項２２記載の組成物。23. The edible plant tissue is a tissue selected from edible plant organs and tissues, cut and pickled edible foods, and contains trehalose in an amorphous state inside the tissue. 23. The composition according to claim 22, wherein the composition is in the form of a hydrated crystal. 【請求項２４】 殺菌及び／又は乾燥工程を施された請
求項２２又は２３記載の組成物。24. The composition according to claim 22, which has been subjected to a sterilization and / or drying step. 【請求項２５】 組成物が、固状食品である請求項２
２、２３又は２４記載の組成物。25. The composition according to claim 2, wherein the composition is a solid food.
25. The composition according to 2, 23 or 24. 【請求項２６】 固状食品が、歯ごたえのある感触を楽
しむことのできるものである請求項２５記載の組成物。26. The composition according to claim 25, wherein the solid food is capable of enjoying a crunchy feel.