JPH11260301A - Transmission electron microscope - Google Patents

Transmission electron microscope

Info

Publication number
JPH11260301A
JPH11260301A JP10056416A JP5641698A JPH11260301A JP H11260301 A JPH11260301 A JP H11260301A JP 10056416 A JP10056416 A JP 10056416A JP 5641698 A JP5641698 A JP 5641698A JP H11260301 A JPH11260301 A JP H11260301A
Authority
JP
Japan
Prior art keywords
sample
coordinates
luminance
sample stage
area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP10056416A
Other languages
Japanese (ja)
Inventor
Hisashi Otsuka
久史 大塚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP10056416A priority Critical patent/JPH11260301A/en
Publication of JPH11260301A publication Critical patent/JPH11260301A/en
Pending legal-status Critical Current

Links

Abstract

PROBLEM TO BE SOLVED: To efficiently search a field of view for a sample by measuring a total/ average of luminance in sample coordinates on a TV image, comparing the total/ average with a reference value, automatically recording the sample coordinates with as luminance which exceeds the reference value and inappropriate for retrieval, and controlling so that a shift to the following area is carried out, without performing scanning movement for the sample coordinates on arriving at the coordinates with the improper luminance during the sample scanning movement. SOLUTION: When the grid size information is inputted from an input unit A14, coordinates corresponding to a grid hole is displayed on a display device 16, and a sample area to be searched is inputted from an input unit B15. A sample stage drive control mechanism 13 automatically sets the shift quantity and direction of a sample stage 6 suitable for search. In this process, luminance on a TV camera 9 of an electron beam 3 passing through a sample 5 is measured by means of a luminance measuring means 17, so as to be compared with a reference value by means of a determining means 18, and sample position coordinates with luminance which exceeds the reference value and inappropriate for observation is stored in a RAM 20. By preventing the sample stage 6 from passing through these position coordinates, a retrieval time can be shortened.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【0001】[0001]

【発明の属する技術分野】透過電子顕微鏡にて試料の視
野検索を自動的に行う場合、試料ステージ駆動をCPU
制御することにより、目的の試料領域を走査移動して検
索に関する。
BACKGROUND OF THE INVENTION When a visual field search of a sample is automatically performed by a transmission electron microscope, a sample stage is driven by a CPU.
By performing control, the target sample area is scanned and moved to relate to the search.

【0002】[0002]

【従来の技術】このような場合従来の装置においては、
試料の有無,破損状態に関係なく試料が走査されるた
め、例えば支持膜の破損により良好な像観察に適さない
領域等までも検索される可能性があり、検索に多大な時
間を費やす傾向があった。また、無試料領域あるいは試
料の破損した領域を除いて検索したい場合でも、目視に
より該領域を特定しなければならず、この作業は煩雑で
かなりの疲労を伴うものであった。
2. Description of the Related Art In such a case, in a conventional apparatus,
Since the sample is scanned irrespective of the presence / absence of the sample and the state of damage, it may be possible to search for areas that are not suitable for good image observation due to, for example, damage to the support film. there were. Further, even when it is desired to search without a sample-free area or a damaged area of a sample, the area must be visually identified, and this operation is complicated and involves considerable fatigue.

【0003】[0003]

【発明が解決しようとする課題】本発明の目的は、従来
試料の有無あるいは破損状態を目視で確認することによ
り検索領域を特定していた透過電子顕微鏡試料の視野検
索について、TVカメラ等で撮像された画像上の特定領
域における輝度の合計若しくは平均値を計測し、該輝度
が予め決定された基準値を超えた場合、該領域内は検索
に不適切と認識して走査移動することなしに飛ばし、次
領域を走査移動するよう試料ステージの自動制御を行う
ことにより、効率的な試料視野検索が可能な透過電子顕
微鏡を提供することである。
SUMMARY OF THE INVENTION An object of the present invention is to use a TV camera or the like to search for a visual field of a transmission electron microscope sample in which a search area has been specified by visually checking the presence or absence or damage of the sample. Measure the total or average value of the brightness in a specific area on the image obtained, and if the brightness exceeds a predetermined reference value, the area in the area is recognized as inappropriate for the search without scanning and moving. An object of the present invention is to provide a transmission electron microscope capable of performing an efficient sample visual field search by automatically controlling a sample stage so as to skip and scan and move a next area.

【0004】[0004]

【課題を解決するための手段】上記目的を達成させるた
めに、TV画像上の予め決定された試料座標における輝
度の合計若しくは平均を計測する手段と、該輝度と予め
決定された基準値とを演算比較する手段と、該輝度が基
準値を超えた場合、該試料座標は検索不適切と認識され
る手段と、該試料座標を自動的に記録する手段と、試料
を走査移動中に該試料座標に到達した際、該試料座標を
走査移動することなく、次領域に移動するように制御さ
れる手段を備えたことを特徴とする。
In order to achieve the above object, means for measuring the total or average of luminance at predetermined sample coordinates on a TV image, and a method for measuring the luminance and a predetermined reference value Means for calculating and comparing, means for recognizing the sample coordinates as inappropriate when the luminance exceeds a reference value, means for automatically recording the sample coordinates, and means for automatically scanning the sample while moving the sample. When the coordinates reach the coordinates, the sample coordinates are controlled to move to the next area without scanning movement.

【0005】[0005]

【発明の実施の形態】以下、本発明の一実施例を図面を
用いて説明する。図1の1は電子顕微鏡鏡体である。電
子銃2より発生した電子線3を収束するための収束レン
ズ4,試料5が装填される試料ステージ6,対物レンズ
7,投影レンズ8及び試料像を撮像するTVカメラ9が
配置されている。TVカメラ9からの信号はアンプ10
に送られ増幅されてTVモニタ11上に画像として表示
される。
DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention will be described below with reference to the drawings. Reference numeral 1 in FIG. 1 denotes an electron microscope mirror. A converging lens 4 for converging the electron beam 3 generated from the electron gun 2, a sample stage 6 on which a sample 5 is loaded, an objective lens 7, a projection lens 8, and a TV camera 9 for capturing a sample image are arranged. The signal from the TV camera 9 is transmitted to the amplifier 10
Is amplified and displayed as an image on the TV monitor 11.

【0006】さらに、TVカメラ9には輝度計測手段1
7と、計測値を判定する判定手段18が接続されてい
る。前記試料ステージ6には試料の観察領域を移動させ
るため試料ステージ駆動機構12と、該駆動を制御する
試料ステージ駆動制御機構13が接続されている。
Further, the TV camera 9 has a luminance measuring means 1
7 and a judging means 18 for judging a measured value are connected. The sample stage 6 is connected to a sample stage drive mechanism 12 for moving the observation region of the sample and a sample stage drive control mechanism 13 for controlling the drive.

【0007】さらに試料ステージ駆動制御機構13には
規格化されたグリッドのサイズ情報を入力する入力装置
A14と、検索したい試料領域を入力する入力装置B1
5と、入力された領域を表示するための表示装置16が
接続されている。
Further, an input device A14 for inputting standardized grid size information and an input device B1 for inputting a sample area to be searched are input to the sample stage drive control mechanism 13.
5 and a display device 16 for displaying the input area.

【0008】このような構成の装置を用いて試料の自動
検索を行う手順を以下に述べる。先ず、入力装置A14
により規格化されたグリッドサイズ情報を入力(設定
1)すると表示装置16にはグリッド孔に対応した座標
が表示されるので、入力装置B15により該座標におい
て検索したい試料領域を入力する(設定2)。前記グリ
ッドサイズ,試料領域の各情報はCPU19を介して試
料ステージ駆動制御機構13に伝達される。
A procedure for automatically searching for a sample using the apparatus having such a configuration will be described below. First, the input device A14
When the grid size information standardized according to (1) is input (setting 1), the coordinates corresponding to the grid holes are displayed on the display device 16, and the input device B15 is used to input the sample area to be searched at the coordinates (setting 2). . The information on the grid size and the sample area is transmitted to the sample stage drive control mechanism 13 via the CPU 19.

【0009】このとき試料ステージ駆動制御機構13
は、前記情報を基に検索に適したステージ移動量,検索
方向を自動設定する(設定3)。さらに、レンズ制御装置
A21により図2(a)の如く1つのグリッド孔のみの
試料像が結像されるよう倍率も自動設定される。そし
て、グリッド孔が観察領域の中央に位置するように試料
ステージ6を移動する。
At this time, the sample stage drive control mechanism 13
Automatically sets a stage movement amount and a search direction suitable for a search based on the information (setting 3). Further, the magnification is automatically set by the lens control device A21 so that a sample image of only one grid hole is formed as shown in FIG. Then, the sample stage 6 is moved so that the grid hole is located at the center of the observation area.

【0010】一方、電子銃2より放出された電子線3
は、予め試料のない領域を撮像して計測されRAM20
にストアされた基準輝度と同じ値を再現するようにレン
ズ制御装置B22により制御され、グリッド孔に照射さ
れる(手順1)。照射された電子線3は、試料を透過し
TVモニタ11上に電子顕微鏡像を表示する。ここでT
Vカメラ9上の輝度を輝度計測手段17が計測し、計測
情報が判定手段18に送られると、基準値との大小を演
算比較する(手順2)。ここで無試料であったり、図2
(a)の如く破損していたりすると該輝度が基準値を超
えるので、このときの試料位置座標がRAM20にスト
アされ、表示装置16上の座標が図2(c)の如く識別
される(手順3)。
On the other hand, the electron beam 3 emitted from the electron gun 2
Is measured in advance by imaging an area without a sample,
Is controlled by the lens control device B22 so as to reproduce the same value as the reference luminance stored in the grid hole, and is applied to the grid holes (procedure 1). The irradiated electron beam 3 passes through the sample and displays an electron microscope image on the TV monitor 11. Where T
The luminance on the V-camera 9 is measured by the luminance measuring means 17, and when the measurement information is sent to the judging means 18, the magnitude of the measured value is compared with the reference value (Step 2). Here, there is no sample,
If the pixel is damaged as shown in FIG. 2A, the luminance exceeds the reference value. Therefore, the coordinates of the sample position at this time are stored in the RAM 20, and the coordinates on the display device 16 are identified as shown in FIG. 3).

【0011】その後試料ステージ6は、設定2で自動設
定された移動量だけ試料5を移動させ(手順4)、次の
グリッド孔に電子線3が照射される(手順1)。このよ
うに、設定3で設定された領域全体の移動が終了するま
で、手順1から手順4が繰り返され検索を終了する。
Thereafter, the sample stage 6 moves the sample 5 by the movement amount automatically set in the setting 2 (procedure 4), and the next grid hole is irradiated with the electron beam 3 (procedure 1). As described above, until the movement of the entire area set in the setting 3 is completed, the procedures 1 to 4 are repeated, and the search is completed.

【0012】前記検索終了後に、試料ステージ駆動制御
機構13は、手順3で最初に記憶されたグリッド孔が視
野中央に来るように試料ステージ6を制御し、グリッド
孔領域内を移動して検索を行う。検索中の試料微動速度
は、設定1で入力されたグリッドサイズ情報に基づき制
御され、最適な速度で検索が可能である。
After completion of the search, the sample stage drive control mechanism 13 controls the sample stage 6 so that the grid hole initially stored in step 3 is located at the center of the field of view, and moves within the grid hole area to perform the search. Do. The sample fine movement speed during the search is controlled based on the grid size information input in the setting 1, and the search can be performed at an optimum speed.

【0013】最初のグリッド孔の検索が終了すると、手
順3で次に記憶されたグリッド孔が視野中央に移動する
ように試料ステージ6が制御され、移動し引き続き検索
を行う。このようにして、手順3で設定された領域全体
の移動が終了するまで図2(c)の如く繰り返される。
When the search for the first grid hole is completed, the sample stage 6 is controlled so that the grid hole stored next in step 3 moves to the center of the visual field, and the sample stage 6 is moved and the search is continued. In this way, the operation is repeated as shown in FIG. 2C until the movement of the entire area set in the procedure 3 is completed.

【0014】[0014]

【発明の効果】本発明によると、電子顕微鏡の観察視野
検索について、撮影観察に適した視野が自動で設定され
るので、効率のよい観察が可能である。また、観察に適
さない領域は試料ステージが通過しないので、検索時間
を短縮することができる。
According to the present invention, the field of view suitable for photographing and observation is automatically set for the observation field of view of the electron microscope, so that efficient observation is possible. In addition, since the sample stage does not pass through an area that is not suitable for observation, the search time can be reduced.

【図面の簡単な説明】[Brief description of the drawings]

【図1】従来の透過電子顕微鏡と本発明の試料検索装置
を備えた透過電子顕微鏡の説明図。
FIG. 1 is an explanatory view of a conventional transmission electron microscope and a transmission electron microscope equipped with a sample retrieval device of the present invention.

【図2】本発明の一実施例であるTV像で表示された試
料領域の平面図。
FIG. 2 is a plan view of a sample area displayed as a TV image according to an embodiment of the present invention.

【符号の説明】[Explanation of symbols]

1…電子顕微鏡鏡体、2…電子銃、3…電子線、4…収
束レンズ、5…試料、6…試料ステージ、7…対物レン
ズ、8…投影レンズ、9…TVカメラ、10…アンプ、
11…TVモニタ、12…試料ステージ駆動機構、13
…試料ステージ駆動制御機構、14…入力装置A、15
…入力装置B、16…表示装置、17…輝度計測手段、
18…判定手段、19…CPU、20…RAM、21…
レンズ制御装置A、22…レンズ制御装置B。
DESCRIPTION OF SYMBOLS 1 ... Electron microscope body, 2 ... Electron gun, 3 ... Electron beam, 4 ... Convergence lens, 5 ... Sample, 6 ... Sample stage, 7 ... Objective lens, 8 ... Projection lens, 9 ... TV camera, 10 ... Amplifier
11: TV monitor, 12: Sample stage drive mechanism, 13
... Sample stage drive control mechanism, 14 ... Input device A, 15
... Input device B, 16 ... Display device, 17 ... Brightness measuring means,
18 ... determination means, 19 ... CPU, 20 ... RAM, 21 ...
Lens control devices A, 22... Lens control device B.

Claims (2)

【特許請求の範囲】[Claims] 【請求項1】試料ステージと、試料ステージを駆動して
観察領域座標を変化させる試料ステージ駆動手段と、該
座標を記憶する記憶手段と、該座標を画面上の各箇所に
対応させて表示する表示手段と、検索する試料の座標を
設定する手段と、倍率により移動量ならびに移動速度を
変化させるように試料ステージ駆動を制御する試料ステ
ージ駆動制御手段と、試料像を撮影する撮像装置と、撮
像画像上の領域を特定する手段を備えた透過電子顕微鏡
において、撮像した画像上の特定領域における輝度の合
計若しくは平均を計測する手段と、該計測した輝度値と
基準値として予め設定された輝度とを演算比較する手段
と、該演算結果に基づいて試料座標を前記記憶手段によ
って自動的に記憶する手段と、該試料座標を前記表示装
置により色別表示して識別可能にする手段を備えたこと
を特徴とする透過電子顕微鏡。
1. A sample stage, a sample stage driving means for driving the sample stage to change the coordinates of an observation area, a storage means for storing the coordinates, and displaying the coordinates in correspondence with each position on the screen. Display means, means for setting coordinates of a sample to be searched, sample stage drive control means for controlling sample stage drive so as to change a moving amount and a moving speed according to a magnification, an imaging device for taking a sample image, and an imaging device In a transmission electron microscope equipped with a means for specifying an area on an image, a means for measuring the total or average of the luminance in a specific area on a captured image, and a luminance previously set as the measured luminance value and a reference value. Means for comparing and calculating the coordinates, means for automatically storing the sample coordinates by the storage means based on the calculation result, and displaying the sample coordinates by the display device in different colors. Transmission electron microscope characterized by comprising means for enabling identification Te.
【請求項2】請求項1記載の透過電子顕微鏡において、
試料ステージ駆動制御手段は、自動検索するために設定
した試料座標領域に、前記の如く識別され記憶された試
料座標が含まれた場合、試料ステージが該試料座標を走
査移動することなく、識別された試料座標以外の試料座
標領域を移動するように制御することを特徴とする透過
電子顕微鏡。
2. The transmission electron microscope according to claim 1, wherein
The sample stage drive control means, when the sample coordinates identified and stored as described above are included in the sample coordinate area set for automatic search, the sample stage is identified without scanning and moving the sample coordinates. A transmission electron microscope characterized in that it is controlled to move a sample coordinate area other than the sample coordinates.
JP10056416A 1998-03-09 1998-03-09 Transmission electron microscope Pending JPH11260301A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10056416A JPH11260301A (en) 1998-03-09 1998-03-09 Transmission electron microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10056416A JPH11260301A (en) 1998-03-09 1998-03-09 Transmission electron microscope

Publications (1)

Publication Number Publication Date
JPH11260301A true JPH11260301A (en) 1999-09-24

Family

ID=13026529

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10056416A Pending JPH11260301A (en) 1998-03-09 1998-03-09 Transmission electron microscope

Country Status (1)

Country Link
JP (1) JPH11260301A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009009771A (en) * 2007-06-27 2009-01-15 Jeol Ltd Sample transfer method of transmission electron microscope

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009009771A (en) * 2007-06-27 2009-01-15 Jeol Ltd Sample transfer method of transmission electron microscope

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