JPH11246422A - Environmental improving agent in alimentary canal - Google Patents
Environmental improving agent in alimentary canalInfo
- Publication number
- JPH11246422A JPH11246422A JP10375281A JP37528198A JPH11246422A JP H11246422 A JPH11246422 A JP H11246422A JP 10375281 A JP10375281 A JP 10375281A JP 37528198 A JP37528198 A JP 37528198A JP H11246422 A JPH11246422 A JP H11246422A
- Authority
- JP
- Japan
- Prior art keywords
- bran
- improving agent
- wet
- agent
- colorectal cancer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、便秘症の予防や大
腸癌の予防に用いられる消化管内環境改善剤に関するも
のである。TECHNICAL FIELD The present invention relates to a gastrointestinal tract environment improving agent used for preventing constipation and colorectal cancer.
【0002】[0002]
【従来の技術】一般に食物繊維は水に可溶の「水溶性繊
維」と水に不溶の「不溶性繊維」に分類され、物理化学
的特性に従って異なる保健効果を発揮することが知られ
ている。例えば水溶性繊維はコレステロールや糖質吸収
阻害作用などの生理作用を示し、心臓疾患や糖尿病の予
防に有効であると言われている。2. Description of the Related Art Dietary fiber is generally classified into "water-soluble fiber" which is soluble in water and "insoluble fiber" which is insoluble in water, and is known to exert different health effects according to physicochemical characteristics. For example, water-soluble fibers exhibit physiological effects such as cholesterol and carbohydrate absorption inhibitory effects, and are said to be effective in preventing heart disease and diabetes.
【0003】一方、不溶性繊維は、糞便量の増加や消化
管通過時間(消化管内容物滞留時間とも言う)の短縮な
どの作用で便秘予防効果があることが知られている。ま
た、食事中の変異原物質や腸内で産生される二次胆汁酸
などの発癌関連物質の濃度を低下させ、すみやかに吸着
***させるなどの作用も併せ持つことから、腸内環境の
改善を通して大腸癌の予防が期待できるとして注目され
ている(「食物繊維」印南 敏、桐山修八編、1995
年)。上記の不溶性繊維としてはセルロース、ヘミセル
ロースやリグニンなどを構成成分とする小麦や大麦のふ
すま、とうもろこしの外皮等の穀類の糟糠が挙げられ
る。このうち、特に小麦ふすまは古来から知られている
緩下作用とともに、食物繊維の中で最も優れた大腸癌予
防効果を発揮することが動物および人への投与試験や疫
学調査で示されている。このような効果を得るには小麦
ふすまを大量に摂取したり継続した摂取が必要となる
が、小麦ふすまの繊維は硬質で口当たりが悪いなどの嗜
好性が劣るために摂取しにくく、健康食品やシリアルな
どごく一部の食品にのみ使用が限定されている。[0003] On the other hand, it is known that insoluble fiber has an effect of preventing constipation due to actions such as an increase in the amount of feces and a shortening of a gastrointestinal transit time (also referred to as a gastrointestinal contents retention time). It also has the effect of reducing the concentration of carcinogens such as mutagenic substances in the diet and secondary bile acids produced in the intestine, and promptly excreting and excreting it. (Dietary Fiber, Satoshi Innan, Shuhatsu Kiriyama, 1995)
Year). Examples of the insoluble fiber include bran of cereals such as wheat or barley bran and corn hulls, which contain cellulose, hemicellulose, lignin or the like as a component. Of these, wheat bran has been shown in animal and human administration studies and epidemiological studies to show the best colon fiber cancer-preventing effect of dietary fiber, in addition to its known laxative effect from ancient times. . In order to obtain such an effect, it is necessary to take a large amount or continuous intake of wheat bran. Its use is limited to only a small portion of foods, such as cereals.
【0005】そこで、小麦ふすまの食品用途拡大のため
に、乾式粉砕機で粒度を小さくして食感を改善すること
が試みられている。しかし、繊維の主体である細胞壁の
多層構造が完全に崩壊されないために、食べた時のザラ
ツキやいがらっぽさが解消されておらず、飲料などテク
スチャーの良否を敏感に感じ取れる食品への応用は困難
とされている。また乾式で微細化した小麦ふすまは、便
秘や大腸癌予防にとって重要な消化管内滞留時間を短縮
させる効果が低下する欠点が、Hellerらのヒトを対象と
した研究で指摘されている(Am.J. Clin. Nutr., 33, 17
34-1744, 1980)。また、特開昭63−17674号に
は、穀類の糠の薄片状磨砕物よりなる食物繊維が開示さ
れているが、本願の課題である消化管内の環境改善効果
については記載されていない。[0005] In order to expand the use of wheat bran for food, attempts have been made to improve the texture by reducing the particle size using a dry pulverizer. However, since the multilayer structure of the cell wall, which is mainly composed of fibers, is not completely collapsed, graininess and irritation when eaten are not eliminated, and application to foods such as beverages where the texture is sensitive can be sensed. Is considered difficult. Heller et al. Have pointed out that dry and fine-grained wheat bran has a reduced effect on reducing the digestive tract retention time, which is important for constipation and colorectal cancer prevention (Am. J . Clin. Nutr., 33, 17
34-1744, 1980). Japanese Patent Application Laid-Open No. 63-17767 discloses a dietary fiber composed of flaky ground cereal bran, but does not describe the effect of improving the environment in the digestive tract, which is the subject of the present application.
【0006】[0006]
【発明が解決しようとする課題】本発明者らは、もとも
と消化管内容物滞留時間の短縮効果が高い穀類の糟糠
を、その効果を保持した上にしかも良好な食感を併せ持
つ消化管内環境改善剤を提供することを課題とした。DISCLOSURE OF THE INVENTION The present inventors have developed a cereal rice bran that has a high effect of shortening the residence time of the contents of the digestive tract from the beginning, while improving the environment in the gastrointestinal tract while maintaining the effect and having a good texture. It was an object to provide an agent.
【0007】[0007]
【課題を解決するための手段】上記課題について本発明
者らが研究を進めたところ、穀類の糟糠、特に小麦ふす
まを湿式磨砕処理によって微細化した薄片状磨砕物が、
良好な食感と高い消化管内容物滞留時間の短縮効果を併
せ持つことを見出し本発明を完成した。Means for Solving the Problems The present inventors have conducted research on the above-mentioned problems. As a result, a flaky ground material obtained by pulverizing grain bran, particularly wheat bran, by a wet grinding process,
The present invention was found to have both a good texture and a high effect of shortening the residence time of gastrointestinal contents, and completed the present invention.
【0008】すなわち、本発明の消化管内環境改善剤
は、穀類の糟糠を湿式磨砕処理した薄片状磨砕物を有効
成分として含有することを特徴とするものである。[0008] That is, the gastrointestinal tract environment improving agent of the present invention is characterized by containing, as an active ingredient, a flaky ground material obtained by wet-milling cereal grain bran.
【0009】また、本発明の消化管内環境改善剤は、小
麦ふすまを湿式磨砕処理した薄片状磨砕物を有効成分と
して含有することを特徴とするものである。In addition, the digestive tract environment improving agent of the present invention is characterized by containing a flaky ground material obtained by wet-milling wheat bran as an active ingredient.
【0010】また、本発明の消化管内環境改善剤に用い
られる湿式磨砕処理した薄片状磨砕物の粒子径が60μm
以下のものが少なくとも80%以上あることを特徴とする
ものである。The particle size of the wet-milled flaky ground material used in the gastrointestinal tract environment improving agent of the present invention is 60 μm.
The following is characterized by at least 80% or more.
【0011】また、本発明は、上記消化管内環境改善剤
を含有することを特徴とする消化管内環境改善食品であ
る。[0011] The present invention is also a food for improving the environment in the digestive tract, which comprises the above agent for improving the environment in the gastrointestinal tract.
【0012】更に、本発明は、上記消化管内環境改善剤
を有効成分として含有することを特徴とする大腸癌抑制
剤である。Further, the present invention is a colorectal cancer inhibitor comprising the above gastrointestinal tract environment improving agent as an active ingredient.
【0013】また、本発明は、上記大腸癌抑制剤を含有
することを特徴とする大腸癌予防食品である。[0013] The present invention also provides a food for preventing colorectal cancer, which comprises the above agent for suppressing colorectal cancer.
【0014】[0014]
【発明の実施の形態】本発明における薄片状磨砕物と
は、穀類の糟糠を湿式状態で圧縮せん断作用またはずれ
応力の作用を行わせながら磨砕して得られるもので、そ
れぞれの食物繊維の持つ独特の多層構造が、ずれ応力に
より破壊されて薄片状に微細化されたものである。BEST MODE FOR CARRYING OUT THE INVENTION The flaky grinding material in the present invention is obtained by grinding cereal grains bran while performing a compressive shearing action or a shearing action in a wet state. It has a unique multilayer structure that is broken down by shear stress and miniaturized into flakes.
【0015】また、本発明の穀類の糟糠として例えば、
小麦のふすま、大麦のふすま、ライ麦のふすま、とうも
ろこしの外皮等を使用することができるが、小麦ふすま
を用いることがより好ましい。これらの穀類の糟糠は、
そのまま後述する湿式磨砕処理を行ってもよいが、好ま
しくは、澱粉質、蛋白質、脂質、無機質等の夾雑物を除
去し、食物繊維含量を高めてから湿式磨砕処理をする。
また、糟糠を酵素処理、化学的処理、物理的処理のいず
れか、またはこれらの処理を適宜組み合せて行うことに
より、食物繊維を豊富に含む穀類の種皮や果皮の組織構
造を弛緩させつつ、澱粉質、蛋白質、脂質、無機質等の
夾雑物を除去することにより得ることができる。この場
合、酵素処理は、例えばα−アミラーゼ、グルコアミラ
ーゼ等の澱粉分解酵素、プロテアーゼ等の蛋白分解酵
素、リパーゼ等の脂質分解酵素をpH3〜9、温度30〜
100 ℃の条件下に添加させて行われる。化学的処理は、
例えば上記穀類の糟糠に鉱酸、有機酸の水溶液を添加
し、pH2〜5の条件下に加熱するかまたは食品用界面活
性剤を添加し、pH3〜8の条件下に熱処理することによ
り行われる。物理的処理は、例えば上記穀類の糟糠をピ
ンミル、ジェットミル等の粉砕機で適宜粉砕して篩い分
けしたり、水に懸濁して強力に攪拌するなどの操作が行
われる。[0015] Further, as the cereal rice bran of the present invention, for example,
Wheat bran, barley bran, rye bran, corn hulls and the like can be used, but it is more preferable to use wheat bran. These cereal casks are
The wet grinding treatment described below may be performed as it is, but preferably, the wet grinding treatment is performed after removing impurities such as starches, proteins, lipids, and inorganic substances to increase the dietary fiber content.
In addition, by carrying out any of enzymatic treatment, chemical treatment, physical treatment, or a combination of these treatments as appropriate, starch can be used to relax the tissue structure of the seed coat and pericarp of cereals rich in dietary fiber, It can be obtained by removing impurities such as proteins, proteins, lipids, and inorganic substances. In this case, the enzymatic treatment is carried out, for example, using a starch degrading enzyme such as α-amylase and glucoamylase, a protease such as protease, and a lipolytic enzyme such as lipase at pH 3 to 9 and a temperature of 30 to 30.
The addition is performed under the condition of 100 ° C. Chemical treatment
For example, it is carried out by adding an aqueous solution of a mineral acid or an organic acid to the bran of the above-mentioned cereal and heating it under the condition of pH 2 to 5 or adding a surfactant for food and heat-treating it under the condition of pH 3 to 8. . In the physical treatment, for example, operations such as crushing and sieving the above-mentioned cereal bran with a pulverizer such as a pin mill or a jet mill, or suspending and stirring vigorously in water are performed.
【0016】穀類の糟糠またはそれらをさらに精製して
なる食物繊維は、必要に応じてさらに粉砕される。この
粒度は、処理に用いる機械や食物繊維の種類により異な
るが、得られる処理物の品質や処理効率から通常32〜20
0 メッシュの範囲のものを使用することが好ましい。す
なわち、32メッシュより粒度の大きいものでは、後の湿
式磨砕工程における処理を効率的に行うことが困難とな
り、また、200 メッシュを超える粒度の細かいもので
は、湿式磨砕でのグラインダーの間隙をすり抜けるため
に薄片化が不十分となり、良質な食物繊維が得られない
からである。The grain bran or the dietary fiber obtained by further purifying them are further pulverized if necessary. The particle size varies depending on the type of machine or dietary fiber used for the treatment, but is usually 32 to 20 depending on the quality and treatment efficiency of the obtained treated product.
It is preferable to use one having a mesh size of 0. In other words, if the particle size is larger than 32 mesh, it will be difficult to perform the treatment in the subsequent wet grinding process efficiently, and if the fine particle size exceeds 200 mesh, the gap between the grinders in the wet grinding process will be reduced. This is because slicing is insufficient and flakes are insufficient, so that good-quality dietary fiber cannot be obtained.
【0017】次に上記食物繊維を、湿式状態でずれ応力
の作用を行なわせながら磨砕処理する。ここで湿式状態
とは、上記植物繊維を水等の液体に懸濁させた状態を意
味する。この懸濁液の食物繊維濃度は、1〜20重量%が
好ましく、2〜10重量%がさらに好ましい。そして、磨
砕処理は食物繊維を湿式の状態で磨砕する能力を有する
装置であればいずれも使用できるが、中でも粉砕外力と
してひねり動作と圧縮剪断作用を有する挽き臼(バアー
ミル)、粉砕外力として衝撃剪断作用を有するコロイド
ミルなどに分類される装置(食糧工学ハンドブック、寺
本四郎編集、P277〜302 、粉砕機各論参照)が好まし
い。コロイドミルの例として、特開昭63−17674
号で示される磨砕処理装置が好ましいが,粒子径が60μ
m以下のものが少なくとも80%以上ある薄片状磨砕物を
得るにはグラインダーの間隙を精度よく監視制御できる
機種が望ましい。Next, the above-mentioned dietary fiber is ground in a wet state while applying a shear stress. Here, the wet state means a state in which the plant fiber is suspended in a liquid such as water. The dietary fiber concentration of this suspension is preferably from 1 to 20% by weight, more preferably from 2 to 10% by weight. The grinding process can be any device that has the ability to grind the dietary fiber in a wet state. Among them, a grinding mill having a twisting action and a compressive shearing action (Baer mill) as an external grinding force, and a grinding external force as Devices classified as colloid mills having an impact shearing action (see Food Engineering Handbook, edited by Shiro Teramoto, p. 277-302, pulverizers) are preferred. As an example of a colloid mill, see JP-A-63-17767.
Is preferred, but the particle size is 60μ.
In order to obtain at least 80% or more of flake-like ground products having a particle size of less than m, a model that can monitor and control the gap of the grinder with high accuracy is desirable.
【0018】こうして得られた食物繊維はその繊維含量
が40%以上であることが好ましく、70%以上であること
がさらに好ましい。ここでの繊維含量とは酵素重量法と
して知られているProsky法(Official Methods of Anal
ysis, 15th, Ed., Assoc. Off. Chem., Arlington, VA,
Vol., Sec.985.29, 1105, 1990)で測定した値である。The dietary fiber thus obtained preferably has a fiber content of at least 40%, more preferably at least 70%. The fiber content here refers to the Prosky method (Official Methods of Anal
ysis, 15th, Ed., Assoc. Off. Chem., Arlington, VA,
Vol., Sec. 985.29, 1105, 1990).
【0019】上記の穀類の糟糠または糟糠を精製して食
物繊維を高めたものを湿式磨砕処理した薄片状磨砕物
は、そのまま本発明の消化管内環境改善剤または大腸癌
予防剤とすることもできるが、食品や経口医薬品に通常
使用されている添加物を加えてもよい。ここで用いる添
加物としては、糖類、蛋白質、脂質、ビタミン類、植物
抽出物、動物抽出物、ゲル化剤、香料、着色剤等が挙げ
られる。The flaky ground material obtained by subjecting the above-mentioned cereal grains to brass bran or refined brass bran to increase dietary fiber and subjecting it to wet grinding may be directly used as the agent for improving the environment in the digestive tract or the prophylactic agent for colorectal cancer of the present invention. Yes, but additives that are commonly used in foods and oral pharmaceuticals may be added. Examples of additives used herein include sugars, proteins, lipids, vitamins, plant extracts, animal extracts, gelling agents, flavors, coloring agents, and the like.
【0020】本発明の消化管内環境改善剤及び大腸癌予
防剤を医薬として使用する場合の投与量は、投与法、患
者の年齢、体重、容態によって異なるが、経口投与の場
合、成人患者に対して1日あたり、5g〜20g程度とす
ることが好ましい。The dosage of the agent for improving the environment in the gastrointestinal tract and the prophylactic agent for colorectal cancer of the present invention varies depending on the administration method, the age, weight and condition of the patient. It is preferable that the amount is about 5 g to 20 g per day.
【0021】また、本発明の消化管内環境改善剤及び大
腸癌予防剤は、任意の範囲で食品に添加して用いること
ができ、消化管内環境改善食品及び大腸癌予防食品とす
ることができる。本発明の消化管内環境改善剤及び大腸
癌予防剤は、非常に粒子が小さく、口当たりがよいため
広い範囲の食品に適宜含有させることができるが、添加
量は飲料など水分含量の多い食品の場合は0.5 〜5重量
%の範囲で、また菓子など固形の食品の場合は1〜40重
量%添加することが望ましい。食品としては例えば乳酸
菌飲料、発酵乳、豆乳、牛乳、チーズ、アイスクリーム
等の乳製品、果汁飲料、スポーツ飲料等の清涼飲料、ス
ープ類、ビスケット、クッキー等の菓子類やパン等を挙
げることができる。The agent for improving the environment in the gastrointestinal tract and the agent for preventing colorectal cancer of the present invention can be used by adding them to food in an arbitrary range, and can be used as the food for improving the environment in the gastrointestinal tract and the food for preventing colon cancer. The gastrointestinal tract environment improving agent and colorectal cancer preventive agent of the present invention can be appropriately contained in a wide range of foods because they have very small particles and are palatable. Is preferably in the range of 0.5 to 5% by weight, and in the case of solid food such as confectionery, it is desirable to add 1 to 40% by weight. Examples of the food include lactic acid bacteria drinks, fermented milk, soy milk, milk, cheese, dairy products such as ice cream, fruit juice drinks, soft drinks such as sports drinks, soups, biscuits, sweets such as cookies, breads and the like. it can.
【0022】[0022]
【実施例】(実施例1)4kgの小麦ふすま(繊維含量45
%、日清製粉製)を36kgの水に懸濁させ、よく攪拌した
のち200 メッシュの篩に移し、篩上に残ったものを60kg
の水に懸濁させた。この液をコロイドミル(増幸産業製
のセレンマイスター)を使用して磨砕し、微細化物を遠
心分離操作で沈殿として回収した。得られたペーストの
食物繊維含量は固形分あたり61%であった。このペース
トの粒度を粒度分布測定装置(コールターLS230 )で測
定したところ、図1に示したように60μm以下の粒子が
92.7%であった。またこのペーストの水分含量を求め、
市販の果汁飲料に固形分が0.5 %になるように添加して
分散させ、飲用したところ、ざらつき感がなく良好な咽
ごしであった。EXAMPLES (Example 1) 4 kg of wheat bran (fiber content 45
%, Manufactured by Nisshin Flour Milling Co., Ltd.) in 36 kg of water, stirred well, and transferred to a 200-mesh sieve. The residue on the sieve was 60 kg.
In water. This liquid was ground using a colloid mill (Selenium Meister manufactured by Masuko Sangyo), and the micronized product was collected as a precipitate by centrifugation. The dietary fiber content of the obtained paste was 61% per solid. When the particle size of this paste was measured with a particle size distribution analyzer (Coulter LS230), as shown in FIG.
92.7%. Also, the water content of this paste was determined,
When added and dispersed in a commercially available fruit juice so that the solid content became 0.5%, and the beverage was drunk, it was a good throat with no rough feeling.
【0023】[0023]
【図1】FIG.
【0024】(試験例1)小麦ふすまを水洗して繊維含
量を高めた小麦ファイバー(繊維含量70%以上、日東製
粉製)を6重量%となるように水に懸濁させたものを20
kg準備し、加温したのち攪拌して膨潤させた。この溶液
をコロイドミルを使用して磨砕し、多層構造を破壊した
微細化物を遠心分離によって回収し、凍結乾燥した。以
下に原料とした小麦ふすま水洗物:Wheat bran(W
B)、WBを乾式粉砕機(ジェットミル;セイシン工
業)により粉砕処理したもの(DWB)、および上記の
ようにしてWBを湿式で磨砕処理したもの(WWB)の
電子顕微鏡写真をそれぞれ図2(A)、図2(B)およ
び図2(C)に示す 。(Test Example 1) Twenty-five wheat fibers (fiber content of 70% or more, manufactured by Nitto Flour Milling Co., Ltd.) suspended in water so that the fiber content was increased by washing the wheat bran with water were increased to 20%.
kg was prepared, heated, and stirred to swell. This solution was ground using a colloid mill, and the micronized product in which the multilayer structure was broken was recovered by centrifugation and freeze-dried. Wheat bran washing product made from: Wheat bran (W
B), electron micrographs of WB crushed by a dry mill (Jet Mill; Seishin Industry) (DWB), and WB crushed by wet grinding (WWB) as described above are shown in FIG. (A), FIG. 2 (B) and FIG. 2 (C).
【0025】[0025]
【図2】FIG. 2
【0026】WBは多層構造を有する不定形の破砕断片
であることが、DWBは、層状構造を有しながらも全体
的に細かな粒子になっていることが観察された。またW
WBは、層状構造のものはなく繊維が微小薄片化してい
る様子が観察された。It has been observed that WB is an amorphous crushed fragment having a multilayer structure, and that DWB has fine particles as a whole while having a layered structure. Also W
WB did not have a layered structure, and it was observed that the fibers were finely flaked.
【0027】(試験例2)〔水中沈定体積の測定〕 3種の試料(WB、DWB 、WWB )1 gを100ml ビーカーに
それぞれ分取し、50ml程度のイオン交換水を加え、スタ
ーラーを用いて20分間懸濁させた。これを20分間脱気し
た後メスシリンダーに移し、イオン交換水を加えて体積
を100ml とした。一晩室温で静置し、沈降部の体積を測
定した。WWBの水中沈定体積はWBの約2倍で、DW
Bの約3倍であった(表1)。(Test Example 2) [Measurement of sedimentation volume in water] 1 g of each of three kinds of samples (WB, DWB, WWB) was dispensed into a 100 ml beaker, about 50 ml of ion-exchanged water was added, and a stirrer was used. And suspended for 20 minutes. This was degassed for 20 minutes, then transferred to a measuring cylinder, and ion-exchanged water was added to bring the volume to 100 ml. After standing at room temperature overnight, the volume of the settling portion was measured. The settling volume of WWB in water is about twice that of WB,
B was about three times (Table 1).
【0028】(試験例3)〔粒度の測定〕 3種の試料(WB、DWB 、WWB )2.5gを100ml ビーカーに
それぞれ分取し、イオン交換水50mlを加え、6,000 回転
で1分間ホモジナイズしたものをサンプルとした。測定
は粒度分布測定装置で測定し、平均粒度で示した(表
1)。WBの平均粒度は、DWBの約7倍、WWBの約1
2倍であった。(Test Example 3) [Measurement of Particle Size] 2.5 g of each of the three samples (WB, DWB, WWB) was taken in a 100 ml beaker, 50 ml of ion-exchanged water was added, and the mixture was homogenized at 6,000 rpm for 1 minute. Was used as a sample. The measurement was carried out with a particle size distribution measuring device and shown as an average particle size (Table 1). The average particle size of WB is about 7 times that of DWB and about 1 times that of WWB.
It was twice.
【0029】[0029]
【表1】 [Table 1]
【0030】(試験例4)〔消化管内容物滞留時間の測
定〕 1)マーカーの調製:クロム修飾物(固相マーカー)およ
びコバルト−エチレンジアミン四酢酸塩(Co-EDTA 、液
相マーカー) Uden らの方法を用いてクロムを結晶セルロース(オリ
エンタル酵母)に修飾させ、クロム修飾セルロースを調
製した。また、液相マーカーとしてCo-EDTA (東京化
成)を用いた。(Test Example 4) [Measurement of Retention Time of Gastrointestinal Contents] 1) Preparation of Marker: Modified Chromium (Solid Phase Marker) and Cobalt-Ethylenediaminetetraacetate (Co-EDTA, Liquid Phase Marker) Uden et al. Chromium was modified into crystalline cellulose (Oriental yeast) using the method described in (1) to prepare chromium-modified cellulose. In addition, Co-EDTA (Tokyo Kasei) was used as a liquid phase marker.
【0031】2)マーカー添加食の調製 無繊維飼料にクロム−セルロースおよびCo-EDTA を加え
よく混合したものをマーカー添加食とした。2) Preparation of a food with a marker A mixture of chromium-cellulose and Co-EDTA added to a fiberless feed and mixed well was used as a food with a marker.
【0032】6週齢のフィッシャー系の雄ラット(日本
クレア)を購入し、1 群5 匹とした。粉末飼料(オリエ
ンタル酵母製)を与え予備飼育した後、代謝ケージに移
し無繊維飼料を与えた。その後AIN76 組成に従い、実験
飼料中の各食物繊維含量が10%となるように飼料を調製
し、自由摂取させた。実験飼料投与前および実験終了時
(解剖直前)のラット体重の測定結果を表2に示す。実
験期間の初体重および終体重とも各群で有意な差は認め
られず、同様の成長結果を示した。Six-week-old male Fischer rats (CLEA Japan) were purchased and grouped into 5 rats. After feeding with powdered feed (manufactured by Oriental Yeast) and pre-breeding, the animals were transferred to metabolic cages and fed with fiberless feed. Thereafter, the diet was prepared according to the AIN76 composition so that the dietary fiber content in the experimental diet was 10%, and the diet was freely taken. Table 2 shows the measurement results of the rat body weight before administration of the experimental feed and at the end of the experiment (immediately before dissection). No significant difference was observed between the groups in the initial body weight and the final body weight during the experimental period, and similar growth results were shown.
【0033】[0033]
【表2】 [Table 2]
【0034】実験飼料投与13日目の夕方に給餌を中断し
ラットを絶食させ、翌日にマーカー添加食を与えた後、
再度実験飼料を投与した。糞は1時間おきに自動採糞装
置(柴田機器製)で54時間集めた。消化管内容物滞留時
間測定期間中の体重増加量および飼料摂取量を表3に示
す。消化管内容物滞留時間の測定期間中の体重増加量お
よび飼料摂取量は各群とも有意な差は認められなかっ
た。On the evening of the thirteenth day of the administration of the experimental feed, the feeding was interrupted and the rats were fasted.
The experimental feed was administered again. Feces were collected every hour for 54 hours using an automatic feces collection device (manufactured by Shibata Kiki). Table 3 shows the amount of weight gain and feed intake during the measurement of the retention time of the contents of the digestive tract. There was no significant difference in body weight gain and feed intake during the measurement of gastrointestinal contents retention time in each group.
【0035】[0035]
【表3】 [Table 3]
【0036】経時的に採取した糞便はチャック付きビニ
ール袋(ユニパックA-4 )に入れ、一晩冷蔵庫で凍らせ
てから48時間凍結乾燥した後に消化管内容物中のマーカ
ーの測定に用いた。糞便中のクロムおよびコバルトの定
量は、発光分光分析法を用い測定した。消化管内容物滞
留時間の指標として平均滞留時間(MRT:mean reten
tion time )を用いた。固相と液相マーカーのMRTは
モーメントの解析法に従い算出し、その結果を表4に示
した。The feces collected over time were placed in a plastic bag with a zipper (Unipack A-4), frozen in a refrigerator overnight, and lyophilized for 48 hours, and then used for measurement of markers in the contents of the digestive tract. Quantification of chromium and cobalt in feces was measured using emission spectroscopy. Mean residence time (MRT: mean reten
tion time) was used. The MRTs of the solid and liquid phase markers were calculated according to the method of analyzing the moment, and the results are shown in Table 4.
【0037】[0037]
【表4】 [Table 4]
【0038】平均滞留時間(MRT)を算出した結果、
DWB群の固相および水相MRTはそれぞれWB群のそ
れらと比較すると長い傾向を示したが、WWB群の固相
と液相のMRT はWB群と同等の値を示した。またWWB
群のMRTは、DWB群と比較すると有意(p<0.05)にM
RTを短縮させた。As a result of calculating the average residence time (MRT),
The solid phase and aqueous phase MRTs of the DWB group tended to be longer than those of the WB group, respectively, but the solid phase and liquid phase MRTs of the WWB group showed values equivalent to those of the WB group. Also WWB
The MRT of the group was significantly (p <0.05) significantly higher than that of the DWB group.
RT was shortened.
【0039】これらの結果から、従来より言われている
ように乾式で微細化された小麦ふすまはMRTが長くな
るが、本特許のように湿式で微細化した小麦ふすまは粒
度が小さくなってもMRTの短縮効果は低下しないこと
が判った。From these results, it is known that the dry-refined wheat bran has a longer MRT as conventionally known, but the wet-refined wheat bran as in the present invention has a smaller particle size even if it has a smaller particle size. It was found that the effect of shortening the MRT did not decrease.
【0040】(試験例5)〔ラット解剖時の盲腸内容物
の水分含量の測定〕 ラット解剖時に盲腸内容物の水分含量の測定を行った。
その測定結果を表5に示す。(Test Example 5) [Measurement of Water Content of Cecal Content at Rat Dissection] The water content of cecal content was measured at the time of rat dissection.
Table 5 shows the measurement results.
【0041】[0041]
【表5】 [Table 5]
【0042】WB群の盲腸内容物の水分含量は、DWB
群に比べ有意に(p<0.05)高く、WWB群に比べ有意に(p
<0.05)低い値を示した。また、WWB群の盲腸内容物の
水分含量はDWB群に比べ有意に(p<0.05)高い値を示し
た。この結果とWWBの水中沈定体積がWBやDWBよ
り高いこと(表1)も考え合わせると、WWBのMRT
短縮効果が優れているのは高い保水能のためと推定され
る。The water content of the cecal contents of the WB group was determined by DWB
Significantly (p <0.05) higher than the group and significantly (p <0.05) higher than the WWB group.
<0.05) showed a low value. The water content of the cecal contents of the WWB group was significantly (p <0.05) higher than that of the DWB group. Considering this result and the fact that the sedimentation volume of WWB in water is higher than that of WB or DWB (Table 1), WWW MRT
The excellent shortening effect is presumed to be due to the high water retention capacity.
【0043】(試験例6)[大腸発癌抑制試験] 4週齢のCF1系雌マウス(チャールズリバー社)75匹
を1週間予備飼育した後、実験飼料の投与を開始した。
実験飼料には、基礎飼料(AIN76Aを一部改変)にセルロ
ース(CL)、小麦ふすま(WB)、WBを湿式で磨砕
処理したもの(WWB)をそれぞれ20%(W/W)添加した
CL飼料、WB飼料、WWB飼料の3種を用い、飼料と
飲料水はいずれも自由摂取とした。また、実験飼料投与
を開始した週から化学発癌剤投与群としてAzoxymethane
(AOM,シグマ化学製)を10mg/kg体重の用量で各群
20匹ずつ毎週1回、合計6回皮下に行った。なお、非
投与群として各群5匹ずつには生理食塩水を投与した。
33週目に全群のマウスを解剖し、実体顕微鏡下で認め
られた結腸腫瘤を病理組織診断後、腫瘍数を表6に示し
た。(Test Example 6) [Colon Carcinogenesis Inhibition Test] After preliminarily rearing 75 4-week-old CF1 female mice (Charles River) for 1 week, administration of the experimental feed was started.
The experimental feed was prepared by adding 20% (W / W) of cellulose feed (CL), wheat bran (WB), and wet ground WB (WWB) to base feed (partially modified AIN76A) at 20% (W / W). Feed, WB feed, and WWB feed were used, and the feed and drinking water were all freely available. In addition, from the week when the experimental feed administration was started, Azoxymethane was
(AOM, Sigma Chemical Co.) was administered subcutaneously at a dose of 10 mg / kg body weight once a week for a total of 6 times, 20 animals per group. As a non-administration group, physiological saline was administered to 5 animals in each group.
At week 33, the mice in all groups were dissected, and colon tumors observed under a stereoscopic microscope were pathologically diagnosed, and the number of tumors was shown in Table 6.
【0044】[0044]
【表6】 [Table 6]
【0045】化学発癌剤投与群における腫瘍の発生率
は、CL飼料群、WB飼料群、WWB飼料群で差は認め
られなかったが、腫瘍を有するマウス当たりの腫瘍数
は、CL飼料群、WB飼料群と比較してWWB飼料群に
腫瘍数の減少が認められた。なお、化学発癌剤非投与群
については、腫瘍の発生は認められなかった。The incidence of tumor in the group administered with the chemical carcinogen was not different between the CL feed group, the WB feed group, and the WWB feed group, but the number of tumors per tumor-bearing mouse was as follows: A decrease in the number of tumors was observed in the WWB feed group as compared to the feed group. No tumor was observed in the group not administered with the chemical carcinogen.
【0046】[0046]
【発明の効果】本発明の穀類の糟糠を湿式磨砕処理によ
って微細化した薄片状磨砕物は、通常の乾式粉砕処理に
よって微細化した糟糠と比べ優れた消化管内滞留時間の
短縮効果及び大腸癌抑制効果を有するため、これを有効
成分として含有する本発明の消化管内環境改善剤及び大
腸癌抑制剤は、便秘症の予防や大腸癌の予防に極めて有
効である。本発明の消化管内環境改善剤及び大腸癌抑制
剤は、粒度が極めて小さくしかも柔らかい薄片状のため
に従来の乾式粉砕物よりはるかに口当たりがよく、広範
囲の食品に配合して使用することが可能である。特に飲
料のような口当たりや喉ごしなどの食感が重要な流動性
食品に配合する場合には、その効果が顕著なものであ
る。なお、この消化管内環境改善剤及び大腸癌抑制剤の
原料は、食品または食品素材として市販されている小麦
ふすまをはじめ穀類の糟糠を用いているため安全性に問
題はなく、食品などに添加して継続的な摂取が可能であ
る。EFFECTS OF THE INVENTION The flaky ground material obtained by pulverizing cereal grain bran by wet grinding according to the present invention is superior in shortening the residence time in the digestive tract and colorectal cancer compared to fine grain ground by ordinary dry grinding. Since it has an inhibitory effect, the agent for improving the environment in the gastrointestinal tract and the agent for suppressing colorectal cancer of the present invention containing it as an active ingredient are extremely effective for preventing constipation and colorectal cancer. The gastrointestinal tract environment improving agent and colorectal cancer suppressant of the present invention have a very small particle size and are soft and flaky, so that they are much more palatable than conventional dry pulverized products, and can be used in a wide range of foods. It is. The effect is particularly remarkable when blended in a fluid food such as a beverage, in which texture such as mouthfeel and throat is important. The raw materials for the gastrointestinal tract environment improver and colorectal cancer suppressant use wheat bran, which is commercially available as food or food material, and do not pose any safety problems. And continuous intake is possible.
【図1】湿式磨砕処理した小麦ふすまの累積頻度分布を
示す図である。FIG. 1 is a diagram showing the cumulative frequency distribution of wheat bran subjected to wet grinding.
【図2】走査型電子顕微鏡を用い撮影した繊維の粒子の
様子を示す図である。FIG. 2 is a view showing a state of fiber particles photographed using a scanning electron microscope.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 FI A61K 31/00 635 A23L 2/00 F (72)発明者 森山 里夏 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内 (72)発明者 佐藤 美紀子 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内 (72)発明者 境谷 有希子 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内 (72)発明者 渡邉 奈満 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内 (72)発明者 ▲高▼木 陽光 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内 (72)発明者 松本 圭介 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内 (72)発明者 諸富 正己 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内 (72)発明者 内田 和美 東京都港区東新橋1丁目1番19号 株式会 社ヤクルト本社内────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. 6 Identification symbol FI A61K 31/00 635 A23L 2/00 F (72) Inventor Satoka Moriyama 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Stock Association Yakult Honsha (72) Inventor Mikiko Sato 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Stock Company Yakult Honsha (72) Inventor Yukiko Sakaiya 1-1-1-1 Higashi-Shimbashi, Minato-ku, Tokyo Stock Company Yakult Honsha (72) Inventor Nanami Watanabe 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Stock Company Yakult Honsha (72) Inventor ▲ Yoko Takagi 1-1-1, Higashi-Shimbashi, Minato-ku, Tokyo No. 19 Yakult Honsha (72) Inventor Keisuke Matsumoto 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo (72) Inventor Masami Morotomi 1-1, Higashi-Shimbashi, Minato-ku, Tokyo No. 19 stock company Yakult in the Head Office (72) inventor Kazumi Uchida Tokyo, Minato-ku, Higashi-Shimbashi 1-chome No. 1, No. 19 stock company Yakult in the head office
Claims (6)
砕物を有効成分として含有することを特徴とする消化管
内環境改善剤。1. A digestive tract environment improving agent comprising as an active ingredient a flaky ground material obtained by wet-milling cereal grains bran.
徴とする請求項1記載の消化管内環境改善剤。2. The agent for improving the environment in the digestive tract according to claim 1, wherein the rice bran of the cereals is even wheat bran.
砕物で、その粒子径が60μm以下のものが少なくとも80
%以上あることを特徴とする請求項1または請求項2記
載の消化管内環境改善剤。3. A flaky ground material obtained by wet-grinding wheat bran and having a particle size of 60 μm or less is at least 80%.
% Or more.
環境改善剤を含有する消化管内環境改善食品。4. A gastrointestinal tract environment improving food containing the gastrointestinal tract environment improving agent according to claim 1.
環境改善剤を有効成分として含有することを特徴とする
大腸癌予防剤。5. A prophylactic agent for colorectal cancer, comprising the gastrointestinal tract environment improving agent according to claim 1 as an active ingredient.
大腸癌予防食品。6. A colorectal cancer preventive food comprising the colorectal cancer preventive agent according to claim 5.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10375281A JPH11246422A (en) | 1997-12-26 | 1998-12-15 | Environmental improving agent in alimentary canal |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP36696197 | 1997-12-26 | ||
JP9-366961 | 1997-12-26 | ||
JP10375281A JPH11246422A (en) | 1997-12-26 | 1998-12-15 | Environmental improving agent in alimentary canal |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH11246422A true JPH11246422A (en) | 1999-09-14 |
Family
ID=26581850
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10375281A Pending JPH11246422A (en) | 1997-12-26 | 1998-12-15 | Environmental improving agent in alimentary canal |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH11246422A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002338475A (en) * | 2001-05-14 | 2002-11-27 | Nippon Shokuhin Kako Co Ltd | Antitumor composition |
WO2004011014A1 (en) * | 2002-07-25 | 2004-02-05 | Seth Kendall | Ingestible material for the treatment of disease in the human digestive system |
JP2009114122A (en) * | 2007-11-06 | 2009-05-28 | Nisshin Pharma Inc | Immunostimulating composition |
WO2018181644A1 (en) * | 2017-03-29 | 2018-10-04 | 日清ファルマ株式会社 | Method for producing processed bran |
JP2019198281A (en) * | 2018-05-17 | 2019-11-21 | 花王株式会社 | Intestinal flora-improving food and drink |
-
1998
- 1998-12-15 JP JP10375281A patent/JPH11246422A/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002338475A (en) * | 2001-05-14 | 2002-11-27 | Nippon Shokuhin Kako Co Ltd | Antitumor composition |
WO2004011014A1 (en) * | 2002-07-25 | 2004-02-05 | Seth Kendall | Ingestible material for the treatment of disease in the human digestive system |
JP2009114122A (en) * | 2007-11-06 | 2009-05-28 | Nisshin Pharma Inc | Immunostimulating composition |
WO2018181644A1 (en) * | 2017-03-29 | 2018-10-04 | 日清ファルマ株式会社 | Method for producing processed bran |
JPWO2018181644A1 (en) * | 2017-03-29 | 2020-02-13 | 日清ファルマ株式会社 | Manufacturing method of processed bran |
JP2019198281A (en) * | 2018-05-17 | 2019-11-21 | 花王株式会社 | Intestinal flora-improving food and drink |
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