JPH11192216A - Blood component drawing container - Google Patents
Blood component drawing containerInfo
- Publication number
- JPH11192216A JPH11192216A JP10000979A JP97998A JPH11192216A JP H11192216 A JPH11192216 A JP H11192216A JP 10000979 A JP10000979 A JP 10000979A JP 97998 A JP97998 A JP 97998A JP H11192216 A JPH11192216 A JP H11192216A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- container
- plasma
- blood component
- tubular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、臨床検査等に用い
られる血液成分採取用容器に関し、より詳細には、血漿
もしくは血清などの血液成分の分離後に、該血液成分を
分離状態のままで長期間保存できる血液成分採取用容器
に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component collection container used for clinical tests and the like, and more particularly, to a blood component such as plasma or serum after separation of the blood component. The present invention relates to a blood component collection container that can be stored for a long period of time.
【０００２】[0002]
【従来の技術】従来、臨床検査等において血漿もしくは
血清などの血液成分を検体として、該検体中の物質を検
査するに際して、血漿もしくは血清（血漿もしくは血清
のことを、以下、血漿等という）の採取は、注射器も
しくは真空採血管を用いて採血し、血液が導かれた試
験管もしくは採血管を遠心し、血漿等と固形分（例え
ば、血球、血餅）とに分離し、分離された血漿等を採
取する方法が用いられてきた。2. Description of the Related Art Conventionally, when a blood component such as plasma or serum is used as a sample in a clinical test or the like and a substance in the sample is tested, plasma or serum (plasma or serum) is hereinafter referred to as plasma. Sampling is performed by collecting blood using a syringe or a vacuum blood collection tube, centrifuging a test tube or blood collection tube into which blood has been led, separating the blood into plasma and the like and solid components (for example, blood cells and blood clots), and separating the separated plasma. And the like.
【０００３】上記のようにして採取された血漿等が検査
に用いられるが、通常、検査時の異常事態の発生等に備
えて、残りの血漿等は保存される。この場合、遠心分離
した血漿等と固形分とを別々に保存せずに、遠心分離に
用いた試験管もしくは採血管にいれたまま、分離状態で
保存するのが簡便であり、よく用いられる方法である。[0003] The plasma or the like collected as described above is used for the test, but the remaining plasma and the like are usually stored in case an abnormal situation occurs during the test. In this case, it is convenient to store the separated components in a test tube or blood collection tube used for centrifugation separately without storing the centrifuged plasma or the like and the solid content separately. It is.
【０００４】この場合、血漿等と固形分は分離された状
態であるが接触しているので、時間が経つと、固形分か
ら物質が滲出して検査値に影響を及ぼすことが問題とな
っていた。例えば、赤血球中から乳酸脱水素酵素（ＬＤ
Ｈ）のような酵素やＫ（カリウム）などが滲出して検査
値に影響を及ぼすことが問題となる。In this case, since plasma and the like are separated from the solid content but in contact with each other, there is a problem that, over time, the substance oozes out of the solid content and affects the test value. . For example, lactate dehydrogenase (LD)
The problem is that enzymes such as H) and K (potassium) ooze out and affect the test values.
【０００５】また、遠心分離に際して、血液に、例え
ば、比重１．０３〜１．０８、粘度５０００〜５０００
００センチポイズの塩素化ポリブテン（特公昭６１−１
６０２３号公報）のような、所謂、血清または血漿分離
剤を添加した後、遠心分離し、血漿等と固形分の間に上
記血清または血漿分離剤の隔壁を形成させる方法も、大
いに普及しているが、この方法においても、遠心分離後
そのままの状態で一週間放置すると、隔壁の下の層の赤
血球からＬＤＨやＫが遊離してくるため、血漿等中のＬ
ＤＨやＫの検査値が上昇するという問題があり、不十分
である。During centrifugation, blood has, for example, a specific gravity of 1.03 to 1.08 and a viscosity of 5000 to 5000.
00 centipoise chlorinated polybutene (JP-B-61-1
No. 6023), a method of adding a so-called serum or plasma separating agent after adding a so-called serum or plasma separating agent, followed by centrifugation to form a partition wall of the above-mentioned serum or plasma separating agent between plasma or the like and a solid content has been widely used. However, also in this method, if left as it is after centrifugation for one week, LDH and K are released from erythrocytes in the layer below the partition wall, so that L
There is a problem that the inspection values of DH and K increase, which is insufficient.
【０００６】[0006]
【発明が解決しようとする課題】本発明の目的は、血漿
もしくは血清などの血液成分の分離後に、該血液成分を
分離状態のままで長期間保存できる血液成分採取用容器
を提供することである。SUMMARY OF THE INVENTION An object of the present invention is to provide a blood component collecting container which can store blood components such as plasma or serum after separation of the blood components in a separated state for a long period of time. .
【０００７】[0007]
【課題を解決するための手段】請求項１記載の発明の血
液成分採取用容器は、有底の管状容器と、該容器内に配
置された有底の筒状体とからなり、該筒状体の底部は直
径０．１〜１０μｍの複数の孔を有する材料から構成さ
れると共に液体透過性であることを特徴とする。The blood component collecting container according to the present invention comprises a bottomed tubular container and a bottomed tubular body disposed in the container. The bottom of the body is composed of a material having a plurality of pores having a diameter of 0.1 to 10 μm and is characterized by being liquid-permeable.
【０００８】すなわち、請求項１記載の発明にかかる血
液成分採取用容器では、上記筒状体内に血液を導入し、
必要に応じて遠心分離すると、該筒状体の底部は直径
０．１〜１０μｍの複数の孔を有する材料から構成され
ているので、赤血球等の固形分は、該筒状体の中に残
り、また、該底部が液体透過性であるので、血漿等は該
筒状体外に出て、上記管状容器内に溜まる。従って、血
漿等と固形分とが接触しない状態となるので、分離状態
のままで長期間保存しても固形分から遊離する物質が血
漿等に混入することがないため、検査値に影響なく血漿
等を保存できる。That is, in the blood component collecting container according to the first aspect of the present invention, blood is introduced into the cylindrical body,
When centrifuged as necessary, the bottom of the cylindrical body is made of a material having a plurality of pores having a diameter of 0.1 to 10 μm, so that solids such as red blood cells remain in the cylindrical body. In addition, since the bottom is liquid-permeable, plasma and the like exit the cylindrical body and accumulate in the tubular container. Therefore, since the plasma and the like do not come into contact with the solid content, the substance released from the solid content does not enter the plasma and the like even when stored in a separated state for a long period of time. Can be saved.
【０００９】なお、上記筒状体は管状容器の径方向のほ
ぼ中央部に配置されるのが好ましいが、上記筒状体をこ
のような位置に固定する方法は、特には限定されるわけ
ではないが、例えば、以下の三つの方法が挙げられる。
管状容器の上端開口を封止する蓋体を用いると共に該
蓋体の底面部に筒状体の上端部を固定する。上記固定方
法としては、接着剤を用いる方法が挙げられる。管状
容器の上端開口に嵌合する栓体を用いると共に該栓体の
底面部に筒状体の上端部を固定する。上記固定方法とし
ては、接着剤を用いる方法、該栓体に筒状体の上端部を
ねじ込む方法、などが挙げられる。筒状体の上端部
に、管状容器の開口の周縁に係合して筒状体を吊り下げ
できるような係合部を設ける。It is preferable that the tubular body is disposed substantially at the center of the tubular container in the radial direction, but the method of fixing the tubular body at such a position is not particularly limited. However, for example, the following three methods can be mentioned.
A lid is used to seal the upper end opening of the tubular container, and the upper end of the tubular body is fixed to the bottom of the lid. Examples of the fixing method include a method using an adhesive. A stopper is fitted to the upper end opening of the tubular container, and the upper end of the tubular body is fixed to the bottom of the stopper. Examples of the fixing method include a method using an adhesive, a method of screwing the upper end of the tubular body into the plug, and the like. An engagement portion is provided at the upper end of the tubular body so as to engage with the peripheral edge of the opening of the tubular container so that the tubular body can be suspended.
【００１０】請求項２記載の発明の血液成分採取用容器
は、有底の管状容器と、該容器内に配置された有底の筒
状体とからなり、該筒状体の底部は液体透過性であり、
該筒状体の内部下方に直径０．１〜２００μｍの親水性
微粒子層が存在していることを特徴とする。[0010] The blood component collecting container according to the second aspect of the present invention comprises a bottomed tubular container and a bottomed tubular body disposed in the container, and the bottom of the tubular body is liquid permeable. Sex
It is characterized in that a hydrophilic fine particle layer having a diameter of 0.1 to 200 μm exists below the inside of the cylindrical body.
【００１１】すなわち、請求項２記載の発明にかかる血
液成分採取用容器では、上記筒状体内に血液を導入し、
必要に応じて遠心分離すると、該筒状体の内部下方に直
径０．１〜２００μｍの親水性微粒子層が存在している
ので、赤血球等の固形分は親水性微粒子層上または該層
の表層部内に残り、また、該筒状体の底部は液体透過性
であるので、血漿等は親水性微粒子層を通過し該底部か
ら該筒状体外に出て、上記管状容器内に溜まる。従っ
て、血漿等と固形分とが接触しない状態となるので、分
離状態のままで長期間保存しても固形分から遊離する物
質が血漿等に混入することがないため、検査値に影響な
く血漿等を保存できる。That is, in the blood component collection container according to the second aspect of the present invention, blood is introduced into the cylindrical body,
When centrifuged as necessary, since a hydrophilic fine particle layer having a diameter of 0.1 to 200 μm is present below the inside of the cylindrical body, solids such as erythrocytes are deposited on the hydrophilic fine particle layer or on the surface layer of the layer. Since the liquid remains permeable at the bottom of the tubular body, and the bottom of the tubular body is liquid-permeable, plasma and the like pass through the hydrophilic fine particle layer, exit the tubular body from the bottom, and accumulate in the tubular container. Therefore, since the plasma and the like do not come into contact with the solid content, the substance released from the solid content does not enter the plasma and the like even when stored in a separated state for a long period of time. Can be saved.
【００１２】なお、上記筒状体は管状容器の径方向のほ
ぼ中央部に配置されるのが好ましいが、上記筒状体をこ
のような位置に固定する方法は、請求項１記載の発明の
場合と同様である。It is preferable that the tubular body is disposed substantially at the center in the radial direction of the tubular container. A method for fixing the tubular body at such a position is described in claim 1 of the present invention. Same as in the case.
【００１３】請求項３記載の発明の血液成分採取用容器
は、上記親水性微粒子層内に、上端に開口を有する赤血
球不透過性の袋状部がその開口を残して埋め込まれると
共に、上記親水性微粒子層の上面が該袋状部の開口に向
かって傾斜していることを特徴とする請求項２記載の血
液成分採取用容器である。According to a third aspect of the present invention, there is provided the blood component collecting container, wherein a red blood cell impermeable bag-like portion having an opening at an upper end is embedded in the hydrophilic fine particle layer while leaving the opening, and the hydrophilic fine particle layer is filled with the hydrophilic particle. The blood component collection container according to claim 2, wherein the upper surface of the conductive fine particle layer is inclined toward the opening of the bag-shaped portion.
【００１４】すなわち、請求項３記載の発明にかかる血
液成分採取用容器では、上記筒状体内に血液を導入し、
必要に応じて遠心分離すると、該筒状体の内部下方に直
径０．１〜２００μｍの親水性微粒子層が存在している
ので、赤血球は親水性微粒子層上または該層の表層部内
に溜まるが、親水性微粒子層内に、上端に開口を有する
赤血球不透過性の袋状部がその開口を残して埋め込まれ
ると共に、上記親水性微粒子層の上面が該袋状部の開口
に向かって傾斜しているので、親水性微粒子層上に溜ま
る赤血球は該層上をころがり落ちて該袋状部に溜まる。
請求項２記載の発明の場合は、多量の血液を分離しよう
とすると、親水性微粒子層上に固形分が溜まってくると
血漿の分離性が徐々に落ちてくるが、請求項３の発明の
場合は、赤血球は袋状部に溜まり、袋状部の周囲の親水
性微粒子層上には溜まりにくいので、血漿の分離性が低
下しにくい。また、血漿の長期間保存安定性について
は、請求項２の発明の場合と同様である。That is, in the blood component collecting container according to the third aspect of the present invention, blood is introduced into the cylindrical body,
When centrifuged as necessary, since the hydrophilic fine particle layer having a diameter of 0.1 to 200 μm is present below the inside of the cylindrical body, erythrocytes accumulate on the hydrophilic fine particle layer or in the surface layer portion of the layer. An erythrocyte-impermeable bag-like portion having an opening at the upper end is embedded in the hydrophilic fine-particle layer while leaving the opening, and the upper surface of the hydrophilic fine-particle layer is inclined toward the opening of the bag-like portion. Therefore, red blood cells accumulated on the hydrophilic fine particle layer roll down on the layer and accumulate in the bag-like portion.
In the case of the second aspect of the invention, when a large amount of blood is to be separated, the separability of plasma gradually decreases as solids accumulate on the hydrophilic fine particle layer. In this case, red blood cells accumulate in the bag-like portion and hardly accumulate on the hydrophilic fine particle layer around the bag-like portion, so that the plasma separation property is not easily reduced. The long-term storage stability of plasma is the same as that of the second aspect of the present invention.
【００１５】請求項４記載の発明の血液成分採取用容器
は、有底の管状容器と、該容器内に配置された有底の筒
状体とからなり、該筒状体の底部は、底部先端に向かっ
て傾斜した傾斜部と該傾斜部に連設する袋状部とから構
成され、該傾斜部は直径０．１〜１０μｍの複数の孔を
有する材料が用いられ液体透過性であり、該袋状部は赤
血球不透過性であることを特徴とする。According to a fourth aspect of the present invention, there is provided a blood component collection container comprising a bottomed tubular container and a bottomed tubular body disposed in the container, wherein the bottom of the tubular body is a bottom portion. It is composed of an inclined portion inclined toward the tip and a bag-shaped portion connected to the inclined portion, and the inclined portion is made of a material having a plurality of holes having a diameter of 0.1 to 10 μm and is liquid-permeable, The pouch is characterized by being erythrocyte impermeable.
【００１６】すなわち、請求項４記載の発明にかかる血
液成分採取用容器では、上記筒状体内に血液を導入し、
必要に応じて遠心分離すると、該筒状体の底部は、底部
先端に向かって傾斜した傾斜部と該傾斜部に連設する袋
状部とから構成され、該傾斜部は直径０．１〜１０μｍ
の複数の孔を有する材料が用いられ液体透過性であるの
で、血漿は該傾斜部より該筒状体外に出て、上記管状容
器内に溜まる。一方、該傾斜部に溜まる赤血球は該傾斜
部上をころがり落ちて該袋状部に溜まる。従って、多量
の血液を分離しても、赤血球は袋状部に溜まり、傾斜部
には溜まりにくいので、血漿の分離性が低下しにくい。
また、血漿の長期間保存安定性については、請求項１〜
３の発明の場合と同様である。That is, in the blood component collection container according to the fourth aspect of the present invention, blood is introduced into the cylindrical body,
When centrifuged as needed, the bottom of the tubular body is composed of an inclined portion inclined toward the bottom tip and a bag-shaped portion connected to the inclined portion, and the inclined portion has a diameter of 0.1 to 0.1 mm. 10 μm
Since the material having a plurality of holes is used and is liquid-permeable, the plasma exits the cylindrical body through the inclined portion and accumulates in the tubular container. On the other hand, the red blood cells accumulated in the inclined portion roll down on the inclined portion and accumulate in the bag-shaped portion. Therefore, even if a large amount of blood is separated, red blood cells accumulate in the bag-like portion and hardly accumulate on the inclined portion, so that the separability of plasma does not easily decrease.
Further, regarding the long-term storage stability of plasma, claims 1 to
This is the same as the case of the third invention.
【００１７】請求項５記載の発明の血液成分採取用容器
は、上記管状容器の上端開口を閉成するように取り付け
られた栓体を更に備え、上記管状容器および上記筒状体
内が減圧にされていることを特徴とする請求項１〜４の
いずれか１項に記載の血液成分採取用容器である。A blood component collecting container according to a fifth aspect of the present invention further comprises a stopper attached so as to close an upper end opening of the tubular container, and the tubular container and the cylindrical body are evacuated. The blood component collection container according to any one of claims 1 to 4, characterized in that:
【００１８】すなわち、請求項５記載の発明にかかる血
液成分採取用容器では、請求項１〜４のいずれか１項に
記載の血液成分採取用容器において、上記管状容器の上
端開口を閉成するように栓体が取り付けられており、上
記管状容器および上記筒状体内が減圧にされている。従
って、従来の真空採血管の使用方法と同様に、マルチプ
ル注射針および採血ホルダーを用いて本血液成分採取用
容器に真空採血法によって採血すると、上記管状容器お
よび上記筒状体内が減圧にされているので、減圧の程度
に応じて血液を速やかに採血できると同時に、上記筒状
体内に採血された血液が速やかに分離されて、筒状体内
に固形分が残り、血漿等が管状容器に溜まる。また、多
量の血液を分離する場合には、必要に応じて遠心分離し
てもよい。このように、血漿等と固形分とが接触しない
状態となるので、分離状態のままで長期間保存しても固
形分から遊離する物質が血漿等に混入することがないた
め、検査値に影響なく血漿等を保存できる。That is, in the blood component collecting container according to the fifth aspect of the present invention, in the blood component collecting container according to any one of the first to fourth aspects, the upper end opening of the tubular container is closed. So that the pressure in the tubular container and the tubular body is reduced. Therefore, similar to the conventional method of using a vacuum blood collection tube, when blood is collected from the blood component collection container by a vacuum blood collection method using a multiple injection needle and a blood collection holder, the pressure in the tubular container and the cylindrical body is reduced. Therefore, blood can be quickly collected according to the degree of decompression, and at the same time, blood collected in the cylindrical body is quickly separated, solids remain in the cylindrical body, and plasma and the like accumulate in the tubular container. . When a large amount of blood is separated, centrifugation may be performed as necessary. As described above, since the plasma and the like do not come into contact with the solid content, the substance released from the solid content does not enter the plasma and the like even when stored in a separated state for a long time, so that the test value is not affected. Plasma can be stored.
【００１９】[0019]
【発明の実施の形態】以下、図面を参照しつつ本発明の
血液成分採取用容器の構造例を説明する。図１は、請求
項１記載の発明にかかる血液成分採取用容器の構造例を
示す断面図である。血液成分採取用容器１は、有底の管
状容器２と、該容器２内に配置された有底の筒状体３と
からなり、該筒状体３の底部３ａは直径０．１〜１０μ
ｍの複数の孔を有する材料から構成されている。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, an example of the structure of a blood component collection container according to the present invention will be described with reference to the drawings. FIG. 1 is a sectional view showing a structural example of a blood component collection container according to the first aspect of the present invention. The blood component collection container 1 includes a bottomed tubular container 2 and a bottomed cylindrical body 3 disposed in the container 2, and the bottom 3 a of the cylindrical body 3 has a diameter of 0.1 to 10 μm.
It is composed of a material having a plurality of m holes.
【００２０】上記管状容器２は、ポリエチレンテレフタ
レートなどの合成樹脂やガラスなどの適宜の材料からな
るものを用いることができ、特に限定されるものではな
い。上記筒状体３は、ポリプロピレンなどの合成樹脂や
ガラスなどの適宜の材料からなるものを用いることがで
き、特に限定されるものではない。上記筒状体３の底部
３ａを構成する、直径０．１〜１０μｍの複数の孔を有
する材料とは、例えば、直径０．１〜１０μｍの複数の
孔径を有するフィルター；直径０．１〜１０μｍの複数
の貫通孔を有する多孔プレートなどが挙げられる。上記
底部３ａの筒状体３への固定は、接着剤を用いて行って
もよい。あるいは底部３ａは、筒状体３と一体に形成さ
れていてもよい。さらに、底部３ａは、筒状体３の下端
に配置されている必要は必ずしもなく、下端よりもやや
上方に配置されていてもよい。The tubular container 2 can be made of an appropriate material such as synthetic resin such as polyethylene terephthalate or glass, and is not particularly limited. The cylindrical body 3 can be made of an appropriate material such as synthetic resin such as polypropylene or glass, and is not particularly limited. The material having a plurality of pores having a diameter of 0.1 to 10 μm, which constitutes the bottom 3 a of the cylindrical body 3, is, for example, a filter having a plurality of pores having a diameter of 0.1 to 10 μm; And a porous plate having a plurality of through holes. The fixing of the bottom 3a to the tubular body 3 may be performed using an adhesive. Alternatively, the bottom 3a may be formed integrally with the tubular body 3. Furthermore, the bottom 3a does not necessarily need to be arranged at the lower end of the tubular body 3, and may be arranged slightly above the lower end.
【００２１】なお、請求項１記載の発明において、上記
管状容器２および筒状体３は、断面円形のものに限定さ
れるわけではなく、例えば、断面楕円形のような略円形
のもの、断面四角形、断面三角形も含まれるものとす
る。また、請求項２〜５記載の発明においても、管状容
器および筒状体に含まれる範囲については同様である。In the first aspect of the present invention, the tubular container 2 and the tubular body 3 are not limited to those having a circular cross section. A quadrangle and a triangular cross section are also included. Also in the inventions of claims 2 to 5, the same applies to the range included in the tubular container and the tubular body.
【００２２】図２（ａ）に示すように、上記血液成分採
取用容器１の筒状体３内に血液４を導入すると、図２
（ｂ）に示すように、赤血球等の固形分４ａは、該筒状
体３の中に残り、また、該底部が液体透過性であるの
で、血漿等４ｂは該筒状体３外に出て、上記管状容器２
内に溜まる。従って、血漿等４ｂと固形分４ａとが接触
しない状態となるので、分離状態のままで長期間保存し
ても固形分４ａから遊離する物質が血漿等４ｂに混入す
ることがないため、検査値に影響なく血漿等４ｂを保存
できる。As shown in FIG. 2 (a), when blood 4 is introduced into the cylindrical body 3 of the blood component collecting container 1, FIG.
As shown in (b), solids 4a such as erythrocytes remain in the cylindrical body 3 and the bottom 4 is liquid-permeable, so that the plasma 4b and the like goes out of the cylindrical body 3. And the tubular container 2
Accumulate inside. Therefore, since the plasma 4b and the solid 4a do not come into contact with each other, the substance released from the solid 4a does not enter the plasma 4b even when stored in a separated state for a long time. 4b can be preserved without any effect.
【００２３】なお、上記の操作において、使用する血液
量が少ない場合は、遠心分離する必要はないが、血液量
が多い場合は、分離時間を短くするために遠心分離する
のが好ましい。In the above operation, if the amount of blood to be used is small, there is no need to perform centrifugation, but if the amount of blood is large, it is preferable to perform centrifugation to shorten the separation time.
【００２４】また、上記の操作において、血液を血漿と
血球に分離したい場合には、筒状体３内に予めヘパリン
ナトリウム等の血液抗凝固剤をいれておくのが好まし
い。また、血液を血清と血餅に分離したい場合には、筒
状体３内に予め微粉末シリカのような血液凝固促進剤を
いれておくのが好ましい。また、この点に関しては、請
求項２〜５記載の発明についても同様である。In the above operation, when it is desired to separate blood into plasma and blood cells, it is preferable to put a blood anticoagulant such as heparin sodium in the cylindrical body 3 in advance. When it is desired to separate blood into serum and blood clot, it is preferable to put a blood coagulation accelerator such as finely divided silica in the cylindrical body 3 in advance. In this regard, the same applies to the inventions according to claims 2 to 5.
【００２５】また、以下の図示の構造例においても、管
状容器および筒状体については請求項１記載の発明と同
様の材料で製造される。Also, in the following structural examples shown below, the tubular container and the tubular body are made of the same material as in the first aspect of the present invention.
【００２６】図３は、請求項５記載の発明にかかる血液
成分採取用容器の構造例を示す断面図であり、図１に示
した血液成分採取用容器１と異なる点は、血液成分採取
用容器５では、管状容器２の上端開口２ａが栓体６によ
り閉成されていることにある。栓体６を構成する材料に
ついても特に限定されず、ブチルゴムなどのゴム弾性を
有する天然もしくは合成ゴムからなるものを用いること
ができる。なお、以下の図示の構造例においても、栓体
６を構成する材料については同様の材料で構成される。FIG. 3 is a sectional view showing an example of the structure of a blood component collection container according to the fifth aspect of the present invention. The difference from the blood component collection container 1 shown in FIG. In the container 5, the upper end opening 2 a of the tubular container 2 is closed by a plug 6. The material constituting the plug 6 is not particularly limited, and a material made of natural or synthetic rubber having rubber elasticity such as butyl rubber can be used. In addition, also in the structural example shown below, the material forming the plug 6 is made of the same material.
【００２７】血液成分採取用容器５では、管状容器２お
よび筒状体３内が減圧にされている。この減圧の程度
は、採血針を被採血者の血管に刺通し、該採血針の他端
を栓体６に刺通した場合に、血液が筒状体３内に速やか
に導かれるように選択されている。通常、管状容器２お
よび筒状体３内の減圧の程度は、０．１〜０．９気圧程
度とされる。In the blood component collecting container 5, the pressure in the tubular container 2 and the inside of the cylindrical body 3 are reduced. The degree of the decompression is selected so that when the blood collection needle is pierced into the blood vessel of the blood-collecting subject and the other end of the blood collection needle is pierced into the plug 6, the blood is quickly guided into the cylindrical body 3. ing. Usually, the degree of pressure reduction in the tubular container 2 and the cylindrical body 3 is set to about 0.1 to 0.9 atm.
【００２８】血液成分採取用容器５においては、血液を
筒状体３内に圧力差を利用して吸引し、図４（ａ）に示
すように血液４を筒状体３内に採取することができる。
筒状体３内に採取された血液４は、管状容器２内も減圧
状態になっているため速やかに、管状容器２内に吸引さ
れ、図４（ｂ）に示すように、赤血球等の固形分４ａ
は、該筒状体３の中に残り、また、該底部が液体透過性
であるので、血漿等４ｂは該筒状体３外に出て、上記管
状容器２内に溜まる。従って、血漿等４ｂと固形分４ａ
とが接触しない状態となるので、分離状態のままで長期
間保存しても固形分４ａから遊離する物質が血漿等４ｂ
に混入することがないため、検査値に影響なく血漿等４
ｂを保存できる。In the blood component collecting container 5, blood is sucked into the cylindrical body 3 using a pressure difference, and the blood 4 is collected into the cylindrical body 3 as shown in FIG. Can be.
The blood 4 collected in the tubular body 3 is quickly sucked into the tubular container 2 because the inside of the tubular container 2 is also in a decompressed state, and as shown in FIG. Minute 4a
Remains in the tubular body 3 and the bottom portion is liquid-permeable, so that the plasma 4b or the like goes out of the tubular body 3 and accumulates in the tubular container 2. Therefore, the plasma 4b and the solid 4a
Is not in contact with the solid 4a even when stored in a separated state for a long time.
Because it is not mixed into the plasma
b can be saved.
【００２９】なお、上記の操作において、使用する血液
量が少ない場合は、遠心分離する必要はないが、血液量
が多い場合は、分離時間を短くするために遠心分離する
のが好ましい。In the above operation, if the amount of blood used is small, it is not necessary to perform centrifugation, but if the amount of blood is large, it is preferable to perform centrifugation to shorten the separation time.
【００３０】図５は、請求項２記載の発明にかかる血液
成分採取用容器の構造例を示す断面図である。血液成分
採取用容器７は、有底の管状容器２と、該容器２内に配
置された有底の筒状体８とからなり、該筒状体８の底部
８ａは液体透過性であり、該筒状体８の内部下方に直径
０．１〜２００μｍの親水性微粒子層９が存在してい
る。FIG. 5 is a sectional view showing an example of the structure of a blood component collecting container according to the second aspect of the present invention. The blood component collection container 7 includes a bottomed tubular container 2 and a bottomed tubular body 8 arranged in the container 2, and a bottom 8 a of the tubular body 8 is liquid-permeable, A hydrophilic fine particle layer 9 having a diameter of 0.1 to 200 μm exists below the inside of the cylindrical body 8.
【００３１】上記液体透過性の底部８ａとしては、例え
ば、直径０．１〜１０μｍの複数の孔径を有するフィル
ター；直径０．１〜１０μｍの複数の貫通孔を有する多
孔プレートなどが挙げられる。上記底部８ａの筒状体８
への固定は、接着剤を用いて行ってもよい。あるいは底
部８ａは、筒状体８と一体に形成されていてもよい。Examples of the liquid-permeable bottom portion 8a include a filter having a plurality of pores having a diameter of 0.1 to 10 μm; and a porous plate having a plurality of through holes having a diameter of 0.1 to 10 μm. The cylindrical body 8 of the bottom 8a
Fixing to the substrate may be performed using an adhesive. Alternatively, the bottom 8a may be formed integrally with the tubular body 8.
【００３２】上記直径０．１〜２００μｍの親水性微粒
子は、血液を濾過し、血漿等を分離するためのものであ
り、その大きさにより性能が決まる。該微粒子として
は、親水性のものであり、例えば、親水性官能基が付け
られたポリスチレン、ポリ塩化ビニル、シリカ、酸化
鉄、多糖類などが挙げられる。The hydrophilic fine particles having a diameter of 0.1 to 200 μm are for filtering blood and separating plasma and the like, and the performance is determined by the size. The fine particles are hydrophilic and include, for example, polystyrene, polyvinyl chloride, silica, iron oxide, and polysaccharides having a hydrophilic functional group.
【００３３】図６（ａ）に示すように、上記血液成分採
取用容器７の筒状体８内に血液４を導入すると、図６
（ｂ）に示すように、赤血球等の固形分４ａは、親水性
微粒子層９上または該層９の表層部内に残り、また、該
筒状体８の底部８ａは液体透過性であるので、血漿等４
ｂは親水性微粒子層９を通過し該底部８ａから該筒状体
８外に出て、上記管状容器２内に溜まる。従って、血漿
等４ｂと固形分４ａとが接触しない状態となるので、分
離状態のままで長期間保存しても固形分４ａから遊離す
る物質が血漿等４ｂに混入することがないため、検査値
に影響なく血漿等４ｂを保存できる。As shown in FIG. 6 (a), when the blood 4 is introduced into the cylindrical body 8 of the blood component collecting container 7, FIG.
As shown in (b), the solids 4a such as red blood cells remain on the hydrophilic fine particle layer 9 or in the surface layer of the layer 9, and the bottom 8a of the cylindrical body 8 is liquid permeable. Plasma 4
b passes through the hydrophilic fine particle layer 9, exits the cylindrical body 8 from the bottom 8 a, and accumulates in the tubular container 2. Therefore, since the plasma 4b and the solid 4a do not come into contact with each other, the substance released from the solid 4a does not enter the plasma 4b even when stored in a separated state for a long time. 4b can be preserved without any effect.
【００３４】また、血液成分採取用容器７においても、
図７に示すように、好ましくは、管状容器２の上端開口
２ａが栓体６により閉成され、管状容器２内が減圧状態
とされる。血液４を筒状体８内に圧力差を利用して吸引
し、図８（ａ）に示すように血液４を筒状体８内に採取
することができる。筒状体８内に導入された血液４は、
管状容器２内も減圧状態になっているため速やかに、親
水性微粒子層９上または該層９の表層部内に赤血球等の
固形分４ａが残り、血漿等４ｂは親水性微粒子層９を通
過し該筒状体８外に出て、上記管状容器２内に溜まる。
従って、血漿等４ｂと固形分４ａとが接触しない状態と
なる。In the blood component collection container 7,
As shown in FIG. 7, preferably, the upper end opening 2a of the tubular container 2 is closed by the stopper 6, and the inside of the tubular container 2 is evacuated. Blood 4 is sucked into cylindrical body 8 using a pressure difference, and blood 4 can be collected in cylindrical body 8 as shown in FIG. The blood 4 introduced into the cylindrical body 8
Since the inside of the tubular container 2 is also in a decompressed state, solids 4a such as erythrocytes remain immediately on the hydrophilic fine particle layer 9 or in the surface layer of the layer 9, and the plasma 4b passes through the hydrophilic fine particle layer 9. It goes out of the cylindrical body 8 and accumulates in the tubular container 2.
Therefore, the plasma 4b and the solids 4a do not come into contact with each other.
【００３５】図９は、請求項３記載の発明にかかる血液
成分採取用容器１０の構造例を示す断面図である。血液
成分採取用容器１０は、有底の管状容器２と、該容器２
内に配置された有底の筒状体１１とからなり、該筒状体
１１の底部１１ａは液体透過性であり、該筒状体１１の
内部下方に直径０．１〜２００μｍの親水性微粒子層９
が存在している。そして、上記親水性微粒子層９内に、
上端に開口１２ａを有する赤血球不透過性の袋状部１２
がその開口１２ａを残して埋め込まれると共に、上記親
水性微粒子層９の上面が該袋状部１２の開口１２ａに向
かって傾斜している。この傾斜角度としては５度以上が
好ましい。FIG. 9 is a sectional view showing a structural example of the blood component collecting container 10 according to the third aspect of the present invention. The blood component collection container 10 includes a bottomed tubular container 2 and the container 2.
And a bottom portion 11a of the tube body 11 which is liquid-permeable and has a hydrophilic fine particle having a diameter of 0.1 to 200 μm below the inside of the tube body 11. Layer 9
Exists. Then, in the hydrophilic fine particle layer 9,
Red blood cell impermeable bag-like portion 12 having an opening 12a at the upper end
Are embedded while leaving the opening 12a, and the upper surface of the hydrophilic fine particle layer 9 is inclined toward the opening 12a of the bag-like portion 12. The inclination angle is preferably 5 degrees or more.
【００３６】上記液体透過性の底部１１ａとしては、例
えば、直径０．１〜１０μｍの複数の孔径を有するフィ
ルター；直径０．１〜１０μｍの複数の貫通孔を有する
多孔プレートなどが挙げられる。上記底部１１ａの筒状
体１１への固定は、接着剤を用いて行ってもよい。ある
いは底部１１ａは、筒状体１１と一体に形成されていて
もよい。Examples of the liquid-permeable bottom portion 11a include a filter having a plurality of pores having a diameter of 0.1 to 10 μm; and a porous plate having a plurality of through holes having a diameter of 0.1 to 10 μm. The fixing of the bottom portion 11a to the tubular body 11 may be performed using an adhesive. Alternatively, the bottom portion 11a may be formed integrally with the tubular body 11.
【００３７】上記袋状部１２は赤血球不透過性であり、
構成材料としては、例えば、ポリエチレンテレフタレー
ト、塩化ビニル、ポリエチレン、ポリスチレン、ポリプ
ロピレンなどの合成樹脂；ガラス等の無機物；鉄、アル
ミニウムなどの金属などが挙げられる。袋状部１２の容
積は、血液成分採取用容器１０に導入される血液量によ
って決まり、該血液量の５０％程度でよい。The bag-like portion 12 is impermeable to red blood cells,
Examples of the constituent materials include synthetic resins such as polyethylene terephthalate, vinyl chloride, polyethylene, polystyrene, and polypropylene; inorganic substances such as glass; and metals such as iron and aluminum. The volume of the bag-like portion 12 is determined by the amount of blood introduced into the blood component collection container 10, and may be about 50% of the blood amount.
【００３８】図１０（ａ）に示すように、上記血液成分
採取用容器１０の筒状体１１内に血液４を導入すると、
赤血球４ａは親水性微粒子層９上または該層の表層部内
に一旦溜まるが、傾斜した親水性微粒子層９上をころが
り落ちて、図１０（ｂ）に示すように、袋状部１２に溜
まる。血漿４ｂは親水性微粒子層９を通過し筒状体１１
外に出て、上記管状容器２内に溜まる。従って、血漿４
ｂと赤血球４ａとが接触しない状態となるので、分離状
態のままで長期間保存しても赤血球４ａから遊離する物
質が血漿４ｂに混入することがないため、検査値に影響
なく血漿４ｂを保存できる。このように、血液成分採取
用容器１０の場合は、赤血球４ａは袋状部１２に溜ま
り、袋状部１２の周囲の親水性微粒子層９上には溜まり
にくいので、多量の血液を処理しても、血漿４ｂの分離
性が低下しにくい。As shown in FIG. 10A, when blood 4 is introduced into the cylindrical body 11 of the blood component collecting container 10,
The red blood cells 4a temporarily accumulate on the hydrophilic fine particle layer 9 or in the surface layer portion of the layer, but roll down on the inclined hydrophilic fine particle layer 9 and accumulate in the bag-like portion 12 as shown in FIG. The plasma 4b passes through the hydrophilic fine particle layer 9 and passes through the cylindrical body 11
It goes out and accumulates in the tubular container 2. Therefore, plasma 4
b and the red blood cells 4a do not come into contact with each other, so that even when stored in a separated state for a long period of time, substances released from the red blood cells 4a do not enter the plasma 4b, so that the plasma 4b is stored without affecting the test values. it can. As described above, in the case of the blood component collection container 10, the red blood cells 4 a accumulate in the bag-like portion 12 and hardly accumulate on the hydrophilic fine particle layer 9 around the bag-like portion 12. Also, the separability of the plasma 4b is hardly reduced.
【００３９】また、血液成分採取用容器１０において
も、図１１に示すように、好ましくは、管状容器２の上
端開口２ａが栓体６により閉成され、管状容器２内が減
圧状態とされる。血液４を筒状体１１内に圧力差を利用
して吸引し、図１２（ａ）に示すように血液４を筒状体
１１内に採取することができる。筒状体１１内に導入さ
れた血液４は、管状容器２内も減圧状態になっているた
め速やかに、赤血球４ａが傾斜した親水性微粒子層９上
をころがり落ちて、図１２（ｂ）に示すように、袋状部
１２に溜まる。血漿４ｂは親水性微粒子層９を通過し筒
状体１１外に出て、上記管状容器２内に溜まる。従っ
て、血漿４ｂと赤血球４ａとが接触しない状態となる。In the blood component collecting container 10, as shown in FIG. 11, preferably, the upper end opening 2a of the tubular container 2 is closed by the stopper 6, and the inside of the tubular container 2 is evacuated. . Blood 4 is sucked into cylindrical body 11 using a pressure difference, and blood 4 can be collected in cylindrical body 11 as shown in FIG. Since the blood 4 introduced into the cylindrical body 11 is also in a depressurized state in the tubular container 2, the red blood cells 4 a quickly roll down on the inclined hydrophilic fine particle layer 9, and as shown in FIG. As shown, it accumulates in the bag portion 12. The plasma 4 b passes through the hydrophilic fine particle layer 9, exits the cylindrical body 11, and accumulates in the tubular container 2. Accordingly, the plasma 4b and the red blood cells 4a do not come into contact with each other.
【００４０】図１３は、請求項４記載の発明にかかる血
液成分採取用容器１３の構造例を示す断面図である。血
液成分採取用容器１３は、有底の管状容器２と、該容器
２内に配置された有底の筒状体１４とからなり、該筒状
体１４の底部は、底部先端に向かって傾斜した傾斜部１
５と該傾斜部１５に連設する袋状部１６とから構成され
る。上記傾斜部１５は、直径０．１〜１０μｍの複数の
孔を有する材料からなり液体透過性である。上記傾斜部
１５の材料としては、例えば、直径０．１〜１０μｍの
複数の孔径を有するフィルター；直径０．１〜１０μｍ
の複数の貫通孔を有する多孔プレートなどが挙げられ
る。上記傾斜部１５の筒状体１４への固定は、接着剤を
用いて行ってもよい。あるいは上記傾斜部１５は、筒状
体１４と一体に形成されていてもよい。上記傾斜部１５
の傾斜角度としては５度以上が好ましい。FIG. 13 is a sectional view showing an example of the structure of the blood component collecting container 13 according to the fourth aspect of the present invention. The blood component collection container 13 includes a bottomed tubular container 2 and a bottomed tubular body 14 arranged in the container 2, and the bottom of the tubular body 14 is inclined toward the bottom tip. Inclined part 1
5 and a bag-like portion 16 connected to the inclined portion 15. The inclined portion 15 is made of a material having a plurality of holes having a diameter of 0.1 to 10 μm and is liquid-permeable. Examples of the material of the inclined portion 15 include a filter having a plurality of pore diameters of 0.1 to 10 μm;
And a porous plate having a plurality of through holes. The fixing of the inclined portion 15 to the tubular body 14 may be performed using an adhesive. Alternatively, the inclined portion 15 may be formed integrally with the tubular body 14. The inclined portion 15
Is preferably 5 degrees or more.
【００４１】上記袋状部１６は赤血球不透過性であり、
構成材料としては、例えば、ポリエチレンテレフタレー
ト、塩化ビニル、ポリエチレン、ポリスチレン、ポリプ
ロピレンなどの合成樹脂；ガラス等の無機物；鉄、アル
ミニウムなどの金属などが挙げられる。袋状部１６の容
積は、血液成分採取用容器１３に導入される血液量によ
って決まり、該血液量の５０％程度でよい。The bag-like portion 16 is impermeable to erythrocytes,
Examples of the constituent materials include synthetic resins such as polyethylene terephthalate, vinyl chloride, polyethylene, polystyrene, and polypropylene; inorganic substances such as glass; and metals such as iron and aluminum. The volume of the bag-like portion 16 is determined by the amount of blood introduced into the blood component collection container 13, and may be about 50% of the blood amount.
【００４２】上記袋状部１６の傾斜部１５への固定は、
接着剤を用いて行ってもよい。あるいは上記袋状部１６
は、筒状体１４および傾斜部１５と一体に形成されてい
てもよい。The fixing of the bag-like portion 16 to the inclined portion 15 is as follows.
This may be performed using an adhesive. Alternatively, the bag-like portion 16
May be formed integrally with the tubular body 14 and the inclined portion 15.
【００４３】図１４（ａ）に示すように、上記血液成分
採取用容器１３の筒状体１４内に血液４を導入すると、
赤血球４ａは傾斜部１５をころがり落ちて袋状部１６に
溜まる。血漿４ｂは傾斜部１５より筒状体１４外に出
て、管状容器２内に溜まる。従って、多量の血液を分離
しても、赤血球４ａは袋状部１６に溜まり、傾斜部１５
には溜まりにくいので、血漿の分離性が低下しにくい。
また、血漿４ｂと赤血球４ａとが接触しない状態となる
ので、分離状態のままで長期間保存しても赤血球４ａか
ら遊離する物質が血漿４ｂに混入することがないため、
検査値に影響なく血漿４ｂを保存できる。As shown in FIG. 14A, when blood 4 is introduced into the cylindrical body 14 of the blood component collecting container 13,
The red blood cells 4 a roll down the inclined portion 15 and accumulate in the bag-like portion 16. The plasma 4 b exits the cylindrical body 14 from the inclined portion 15 and accumulates in the tubular container 2. Therefore, even if a large amount of blood is separated, the red blood cells 4a accumulate in the bag-shaped portion 16 and the inclined portion 15
, It is difficult for the plasma to separate.
In addition, since the plasma 4b and the red blood cells 4a do not come into contact with each other, the substance released from the red blood cells 4a does not enter the plasma 4b even when stored in a separated state for a long time.
The plasma 4b can be stored without affecting the test values.
【００４４】また、血液成分採取用容器１３において
も、図１５に示すように、好ましくは、管状容器２の上
端開口２ａが栓体６により閉成され、管状容器２内が減
圧状態とされる。血液４を筒状体１４内に圧力差を利用
して吸引し、図１６（ａ）に示すように血液４を筒状体
１４内に採取することができる。筒状体１４内に導入さ
れた血液４は、管状容器２内も減圧状態になっているた
め速やかに、赤血球４ａが傾斜部１５をころがり落ち
て、図１６（ｂ）に示すように、袋状部１６に溜まる。
血漿４ｂは傾斜部１５を通過し筒状体１４外に出て、上
記管状容器２内に溜まる。従って、血漿４ｂと赤血球４
ａとが接触しない状態となる。In the blood component collection container 13 as well, as shown in FIG. 15, preferably, the upper end opening 2a of the tubular container 2 is closed by the stopper 6, and the inside of the tubular container 2 is evacuated. . The blood 4 can be sucked into the cylindrical body 14 using a pressure difference, and the blood 4 can be collected into the cylindrical body 14 as shown in FIG. Since the blood 4 introduced into the cylindrical body 14 is also in a depressurized state in the tubular container 2, the red blood cells 4a immediately roll down the inclined portion 15, and as shown in FIG. It collects in the shape part 16.
The plasma 4b passes through the inclined portion 15 and goes out of the tubular body 14 and accumulates in the tubular container 2. Therefore, the plasma 4b and the red blood cells 4
a is not in contact.
【００４５】[0045]
【実施例】次に、本発明の具体的な実施例につき説明す
る。 （実施例１）図１に示した血液成分採取用容器１を作製
した。Next, specific embodiments of the present invention will be described. Example 1 A blood component collection container 1 shown in FIG. 1 was prepared.
【００４６】まず、血漿分離剤入り真空採血管（積水化
学工業社製、真空採血管、商品名：インセパック、直径
１５ｍｍ、長さ１００ｍｍ、ポリエチレンテレフタレー
ト製）を用意した。この真空採血管のゴム栓を取り外
し、管内を常圧とし、管状容器２とした。First, a vacuum blood collection tube containing a plasma separating agent (manufactured by Sekisui Chemical Co., Ltd., vacuum blood collection tube, trade name: Insepack, 15 mm in diameter, 100 mm in length, made of polyethylene terephthalate) was prepared. The rubber stopper of the vacuum blood collection tube was removed, and the inside of the tube was set to normal pressure to obtain a tubular container 2.
【００４７】筒状体３として、直径６ｍｍ、長さ６０ｍ
ｍのポリプロピレン製のチューブを用意した。ミリポア
ー社製、ＭＩＬＬＥＸ−ＳＶから孔径５μｍのフィルタ
ーを取り出し、直径１０ｍｍに切り取り、筒状体３の下
端に粘着テープ（積水化学工業社製、商品名：セキスイ
セロテープ５２５）を用いて固定して、底部３ａとし
た。底部３ａの取り付けられた筒状体３の上端を接着剤
を用いて、ブチルゴム製の栓体の底面部に接着し、上記
管状容器２内に挿入し固定し、血液成分採取用容器１を
作製した。The cylindrical body 3 has a diameter of 6 mm and a length of 60 m.
m, a polypropylene tube was prepared. A filter having a pore size of 5 μm was taken out of MILLEX-SV, manufactured by Millipore Co., Ltd., cut out to a diameter of 10 mm, and fixed to the lower end of the cylindrical body 3 with an adhesive tape (manufactured by Sekisui Chemical Co., Ltd., trade name: Sekisui cello tape 525). Bottom 3a. The upper end of the cylindrical body 3 to which the bottom 3a is attached is adhered to the bottom of a butyl rubber stopper using an adhesive, and inserted and fixed in the tubular container 2 to produce the blood component collection container 1. did.
【００４８】被験者Ａ氏の血液を注射器で採血し、その
血液１ｍｌを、注射針を上記栓体に刺通することによ
り、血液成分採取用容器１の筒状体３内に採取した。次
いで、血液成分採取用容器１を遠心分離器（国産遠心器
社製、商品名：Ｈ−２０）を用いて３０００ｒｐｍで１
０分間、遠心分離した。遠心分離後１０分間放置し、そ
の後血液成分採取用容器１を観察した結果、図２（ｂ）
に示したように固形分４ａと血漿４ｂが分離していた。The blood of the subject A was collected with a syringe, and 1 ml of the blood was collected in the cylindrical body 3 of the blood component collecting container 1 by piercing the stopper with the injection needle. Next, the blood component collection container 1 was placed in a centrifuge (manufactured by Domestic Centrifuge Company, trade name: H-20) at 3000 rpm for 1 hour.
Centrifuged for 0 minutes. After centrifugation, the container was left for 10 minutes, and then the blood component collection container 1 was observed.
As shown in the figure, the solids 4a and the plasma 4b were separated.
【００４９】（比較例１）比較のために、血漿分離剤入
り真空採血管（積水化学工業社製、真空採血管、商品
名：インセパック、直径１５ｍｍ、長さ１００ｍｍ、ポ
リエチレンテレフタレート製）を用意した。被験者Ａ氏
の血液を注射器で採血し、その血液１ｍｌを、注射針を
上記真空採血管の栓体に刺通することにより、真空採血
管に採取した。次いで、該真空採血管を遠心分離器（国
産遠心器社製、商品名：Ｈ−２０）を用いて３０００ｒ
ｐｍで１０分間、遠心分離した。遠心分離後１０分間放
置し、その後真空採血管を観察した結果、固形分４ａと
血漿４ｂが分離していたが固形分層の上面と血漿層の下
面とは接触していた。(Comparative Example 1) For comparison, a vacuum blood collection tube containing a plasma separating agent (manufactured by Sekisui Chemical Co., Ltd., vacuum blood collection tube, trade name: Insepack, diameter 15 mm, length 100 mm, polyethylene terephthalate) was prepared. . The blood of the subject A was collected with a syringe, and 1 ml of the blood was collected in a vacuum blood collection tube by inserting an injection needle through the plug of the vacuum blood collection tube. Next, the vacuum blood collection tube was subjected to 3000 r using a centrifuge (trade name: H-20, manufactured by Kokusan Centrifuge Co., Ltd.).
Centrifuged at pm for 10 minutes. After standing for 10 minutes after centrifugation, and observing the vacuum blood collection tube, the solids 4a and plasma 4b were separated, but the upper surface of the solid layer and the lower surface of the plasma layer were in contact.
【００５０】保存安定性試験 実施例１および比較例１の血漿を分離した容器から、血
漿を１００μｌ取り出し、残りの容器はそのまま容器ご
と冷蔵庫に入れ４℃で保存した。上記の１００μｌ取り
出した血漿中のＬＤＨおよびＫ値を測定した。得られた
結果を直後測定値という。さらに、冷蔵庫に入れ保存し
たものから、７日後に血漿を１００μｌ取り出し、血漿
中のＬＤＨおよびＫ値を測定した。得られた結果を７日
後測定値という。直後および７日後の測定値を表１に示
した。表１より、本発明の血液成分採取用容器を用いた
ものでは、直後および７日後の測定値に変化がないが、
比較例１のものでは、７日後の測定値が上昇しているこ
とがわかる。Storage stability test 100 μl of plasma was taken out from the container from which the plasma of Example 1 and Comparative Example 1 were separated, and the remaining containers were put in a refrigerator as they were and stored at 4 ° C. The LDH and K values in the plasma taken out from 100 μl were measured. The obtained result is called a measured value immediately after. Further, 7 days later, 100 μl of plasma was taken out from the refrigerator and stored, and LDH and K values in plasma were measured. The results obtained are referred to as measured values after 7 days. The measured values immediately after and 7 days later are shown in Table 1. According to Table 1, in the case of using the blood component collection container of the present invention, there is no change in the measured values immediately after and 7 days later.
In the case of Comparative Example 1, it can be seen that the measured value after 7 days has increased.
【００５１】[0051]
【表１】 [Table 1]
【００５２】（実施例２）実施例１で用意した血液成分
採取用容器において、管状容器２および筒状体３内を減
圧し、図３に示した血液成分採取用容器５を作製した。
この場合、減圧にあたっては、真空打栓機（共和真空社
製、商品名：ＶＳ−１５０Ａ）を用い、０．８５気圧で
栓体６を打栓し、血液１ｍｌ真空採血し得るように構成
した。(Example 2) In the blood component collection container prepared in Example 1, the pressure inside the tubular container 2 and the cylindrical body 3 was reduced, and the blood component collection container 5 shown in FIG. 3 was produced.
In this case, the pressure was reduced by using a vacuum stopper machine (manufactured by Kyowa Vacuum Co., Ltd., trade name: VS-150A), the stopper 6 was stoppered at 0.85 atm, and 1 ml of blood was collected in vacuum. .
【００５３】血液成分採取用容器５をマルチプル採血針
が取り付けられた真空採血用ホルダーにセットし、マル
チプル採血針を被験者Ａ氏の血管に刺通し、該採血針の
他端を栓体６に刺通して行う常法の真空採血法により、
血液成分採取用容器５に血液を１ｍｌ採血した。その後
１０分間放置し、血液成分採取用容器５を観察した結
果、図４（ｂ）に示したように固形分４ａと血漿４ｂが
分離していた。The blood component collecting container 5 is set in a vacuum blood collecting holder to which a multiple blood collecting needle is attached, and the multiple blood collecting needle is pierced into the blood vessel of the subject A, and the other end of the blood collecting needle is pierced into the stopper 6. By the usual vacuum blood sampling method
1 ml of blood was collected in the blood component collection container 5. After that, the container was left for 10 minutes, and the blood component collection container 5 was observed. As a result, as shown in FIG. 4B, the solid 4a and the plasma 4b were separated.
【００５４】（比較例２）比較のために、血漿分離剤入
り真空採血管（積水化学工業社製、真空採血管、商品
名：インセパック、直径１５ｍｍ、長さ１００ｍｍ、ポ
リエチレンテレフタレート製）を用意した。該真空採血
管をマルチプル採血針が取り付けられた真空採血用ホル
ダーにセットし、マルチプル採血針を被験者Ａ氏の血管
に刺通し、該採血針の他端を栓体に刺通して行う常法の
真空採血法により、真空採血管に血液を１ｍｌ採血し
た。次いで、該真空採血管を遠心分離器（国産遠心器社
製、商品名：Ｈ−２０）を用いて３０００ｒｐｍで１０
分間、遠心分離した。遠心分離後１０分間放置し、その
後真空採血管を観察した結果、固形分４ａと血漿４ｂが
分離していたが固形分層の上面と血漿層の下面とは接触
していた。Comparative Example 2 For comparison, a vacuum blood collection tube containing a blood plasma separating agent (manufactured by Sekisui Chemical Co., Ltd., vacuum blood collection tube, trade name: Insepack, 15 mm in diameter, 100 mm in length, made of polyethylene terephthalate) was prepared. . The vacuum blood collection tube is set in a vacuum blood collection holder to which a multiple blood collection needle is attached, the multiple blood collection needle is pierced into a blood vessel of the subject A, and the other end of the blood collection needle is pierced into a stopper to perform a normal vacuum blood collection. According to the method, 1 ml of blood was collected in a vacuum blood collection tube. Next, the vacuum blood collection tube was centrifuged at 3000 rpm for 10 minutes using a centrifuge (manufactured by Domestic Centrifuge Co., trade name: H-20).
Centrifuged for minutes. After standing for 10 minutes after centrifugation, and observing the vacuum blood collection tube, the solids 4a and plasma 4b were separated, but the upper surface of the solid layer and the lower surface of the plasma layer were in contact.
【００５５】保存安定性試験 実施例２および比較例２の血漿を分離した容器を用い
て、実施例１および比較例１の保存安定性試験と同様に
操作した。得られた結果を表２に示した。表２より、本
発明の血液成分採取用容器を用いたものでは、直後およ
び７日後の測定値に変化がないが、比較例２のもので
は、７日後の測定値が上昇していることがわかる。Storage stability test Using the containers from which the plasma of Example 2 and Comparative example 2 were separated, the same operation as the storage stability test of Example 1 and Comparative example 1 was performed. Table 2 shows the obtained results. Table 2 shows that the measured values immediately after and 7 days after the use of the blood component collection container of the present invention did not change, but the measured values after 7 days increased in the case of Comparative Example 2. Recognize.
【００５６】[0056]
【表２】 [Table 2]
【００５７】（実施例３）図５に示した血液成分採取用
容器７を作製した。Example 3 A blood component collection container 7 shown in FIG. 5 was prepared.
【００５８】まず、血漿分離剤入り真空採血管（積水化
学工業社製、真空採血管、商品名：インセパック、直径
１５ｍｍ、長さ１００ｍｍ、ポリエチレンテレフタレー
ト製）を用意した。この真空採血管のゴム栓を取り外
し、管内を常圧とし、管状容器２とした。First, a vacuum blood collection tube containing a plasma separating agent (manufactured by Sekisui Chemical Co., Ltd., vacuum blood collection tube, trade name: Insepack, 15 mm in diameter, 100 mm in length, made of polyethylene terephthalate) was prepared. The rubber stopper of the vacuum blood collection tube was removed, and the inside of the tube was set to normal pressure to obtain a tubular container 2.
【００５９】筒状体８として、直径６ｍｍ、長さ６０ｍ
ｍのポリプロピレン製のチューブを用意した。ミリポア
ー社製、ＭＩＬＬＥＸ−ＳＶから孔径５μｍのフィルタ
ーを取り出し、直径１０ｍｍに切り取り、筒状体８の下
端に粘着テープ（積水化学工業社製、商品名：セキスイ
セロテープ５２５）を用いて固定して、底部８ａとし
た。筒状体８の中に、カルボキシル基の付いた粒径８μ
ｍのポリスチレン製ゲル（積水化学工業社製、商品名：
ＭＩＣＲＯＮＥＸ）０．２ｇをいれ、親水性微粒子層９
とした。底部８ａの取り付けられた筒状体８の上端を接
着剤を用いて、ブチルゴム製の栓体の底面部に接着し、
上記管状容器２内に挿入し固定し、血液成分採取用容器
７を作製した。The cylindrical body 8 has a diameter of 6 mm and a length of 60 m.
m, a polypropylene tube was prepared. A filter having a pore diameter of 5 μm was taken out of MILLEX-SV manufactured by Millipore, cut out to a diameter of 10 mm, and fixed to the lower end of the cylindrical body 8 with an adhesive tape (manufactured by Sekisui Chemical Co., Ltd., trade name: Sekisui Sero Tape 525). Bottom 8a. 8 μm particle size with carboxyl groups in the cylindrical body 8
m polystyrene gel (Sekisui Chemical Co., Ltd., trade name:
MICRONEX) 0.2 g and the hydrophilic fine particle layer 9
And The upper end of the cylindrical body 8 to which the bottom 8a is attached is adhered to the bottom of the butyl rubber plug using an adhesive,
The container was inserted into the tubular container 2 and fixed to produce a blood component collecting container 7.
【００６０】被験者Ａ氏の血液を注射器で採血し、その
血液１ｍｌを、注射針を上記栓体に刺通することによ
り、血液成分採取用容器７の筒状体３内に採取した。次
いで、血液成分採取用容器７を遠心分離器（国産遠心器
社製、商品名：Ｈ−２０）を用いて３０００ｒｐｍで１
０分間、遠心分離した。遠心分離後１０分間放置し、そ
の後血液成分採取用容器７を観察した結果、図６（ｂ）
に示したように固形分４ａと血漿４ｂが分離していた。The blood of the subject A was collected with a syringe, and 1 ml of the blood was collected in the cylindrical body 3 of the blood component collecting container 7 by piercing the stopper with the injection needle. Next, the blood component collection container 7 was placed in a centrifuge (manufactured by Domestic Centrifuge Co., trade name: H-20) at 3000 rpm for 1 hour.
Centrifuged for 0 minutes. After centrifugation, the sample was left for 10 minutes, and then the blood component collection container 7 was observed.
As shown in the figure, the solids 4a and the plasma 4b were separated.
【００６１】保存安定性試験 実施例３の血漿を分離した容器を用いて、実施例１の保
存安定性試験と同様に操作した。得られた結果を表３に
示した。表３より、本発明の血液成分採取用容器を用い
たものでは、直後および７日後の測定値に変化がないこ
とがわかる。Storage stability test Using the container from which the plasma of Example 3 was separated, the same operation as in the storage stability test of Example 1 was performed. Table 3 shows the obtained results. From Table 3, it can be seen that in the case of using the blood component collection container of the present invention, there is no change in the measured values immediately after and 7 days later.
【００６２】[0062]
【表３】 [Table 3]
【００６３】（実施例４）実施例３で用意した血液成分
採取用容器において、管状容器２および筒状体８内を減
圧し、図７に示した血液成分採取用容器７を作製した。
この場合、減圧にあたっては、真空打栓機（共和真空社
製、商品名：ＶＳ−１５０Ａ）を用い、０．８５気圧で
栓体６を打栓し、血液１ｍｌ真空採血し得るように構成
した。(Example 4) In the blood component collection container prepared in Example 3, the inside of the tubular container 2 and the cylindrical body 8 were depressurized, and the blood component collection container 7 shown in FIG. 7 was produced.
In this case, the pressure was reduced by using a vacuum stopper machine (manufactured by Kyowa Vacuum Co., Ltd., trade name: VS-150A), the stopper 6 was stoppered at 0.85 atm, and 1 ml of blood was collected in vacuum. .
【００６４】血液成分採取用容器７をマルチプル採血針
が取り付けられた真空採血用ホルダーにセットし、マル
チプル採血針を被験者Ａ氏の血管に刺通し、該採血針の
他端を栓体６に刺通して行う常法の真空採血法により、
血液成分採取用容器７に血液を１ｍｌ採血した。その後
１０分間放置し、血液成分採取用容器７を観察した結
果、図８（ｂ）に示したように固形分４ａと血漿４ｂが
分離していた。The blood component collecting container 7 is set in a vacuum blood collecting holder to which a multiple blood collecting needle is attached, and the multiple blood collecting needle is pierced into the blood vessel of the subject A. By the usual vacuum blood sampling method
1 ml of blood was collected in the blood component collection container 7. After that, the container was left for 10 minutes, and the blood component collection container 7 was observed. As a result, as shown in FIG. 8B, the solids 4a and the plasma 4b were separated.
【００６５】保存安定性試験 実施例４の血漿を分離した容器を用いて、実施例１の保
存安定性試験と同様に操作した。得られた結果を表４に
示した。表４より、本発明の血液成分採取用容器を用い
たものでは、直後および７日後の測定値に変化がないこ
とがわかる。Storage stability test Using the container from which the plasma of Example 4 was separated, the same operation as in the storage stability test of Example 1 was performed. Table 4 shows the obtained results. Table 4 shows that in the case of using the container for collecting blood components of the present invention, there is no change in the measured values immediately after and 7 days later.
【００６６】[0066]
【表４】 [Table 4]
【００６７】（実施例５）図９に示した血液成分採取用
容器１０を作製した。Example 5 A blood component collecting container 10 shown in FIG. 9 was prepared.
【００６８】まず、血漿分離剤入り真空採血管（積水化
学工業社製、真空採血管、商品名：インセパック、直径
１５ｍｍ、長さ１００ｍｍ、ポリエチレンテレフタレー
ト製）を用意した。この真空採血管のゴム栓を取り外
し、管内を常圧とし、管状容器２とした。First, a vacuum blood collection tube containing a plasma separating agent (manufactured by Sekisui Chemical Co., Ltd., vacuum blood collection tube, trade name: Insepack, diameter 15 mm, length 100 mm, made of polyethylene terephthalate) was prepared. The rubber stopper of the vacuum blood collection tube was removed, and the inside of the tube was set to normal pressure to obtain a tubular container 2.
【００６９】筒状体１１として、採血量２．５ｍｌの注
射器（テルモ社製、商品名：テルモシリンジ）の注射筒
を用意した。ミリポアー社製、ＭＩＬＬＥＸ−ＬＨから
孔径０．５μｍのサンプレップフィルターを取り出し、
筒状体１１の下端に粘着テープ（積水化学工業社製、商
品名：セキスイセロテープ５２５）を用いて固定して、
底部１１ａとした。筒状体１１の中に、カルボキシル基
の付いた粒径８μｍのポリスチレン製ゲル（積水化学工
業社製、商品名：ＭＩＣＲＯＮＥＸ）０．４ｇをいれ、
親水性微粒子層９とした。親水性微粒子層９の中央に、
ポリプロピレン製１．５ｍｌ容量のサンプルカップ（エ
ッペンドルフ社製）を袋状部１２として上端開口１２ａ
を残して埋め込み、親水性微粒子層９の上面が上記上端
開口１２ａに向かって傾斜しているようにした。このよ
うに得られた筒状体１１の上端を接着剤を用いて、ブチ
ルゴム製の栓体の底面部に接着し、上記管状容器２内に
挿入し固定し、血液成分採取用容器１０を作製した。As the cylindrical body 11, a syringe of a syringe (Termo Syringe, manufactured by Terumo Corporation) with a blood collection amount of 2.5 ml was prepared. Take out a 0.5 μm pore size sunprep filter from MILLEX-LH manufactured by Millipore,
The lower end of the tubular body 11 is fixed with an adhesive tape (manufactured by Sekisui Chemical Co., Ltd., trade name: Sekisui Sero Tape 525),
Bottom 11a. 0.4 g of polystyrene gel (trade name: MICRONEX, manufactured by Sekisui Chemical Co., Ltd.) having a carboxyl group and having a particle size of 8 μm is placed in the cylindrical body 11.
The hydrophilic fine particle layer 9 was obtained. In the center of the hydrophilic fine particle layer 9,
An upper end opening 12 a made of a polypropylene-made 1.5 ml sample cup (manufactured by Eppendorf) as a bag-shaped portion 12.
And the upper surface of the hydrophilic fine particle layer 9 is inclined toward the upper end opening 12a. The upper end of the cylindrical body 11 thus obtained is adhered to the bottom of a butyl rubber stopper using an adhesive, inserted into the tubular container 2 and fixed, thereby producing a blood component collection container 10. did.
【００７０】被験者Ａ氏の血液を注射器で採血し、その
血液１ｍｌを、注射針を上記栓体に刺通することによ
り、血液成分採取用容器１０の筒状体１１内に採取し
た。次いで、血液成分採取用容器１０を遠心分離器（国
産遠心器社製、商品名：Ｈ−２０）を用いて３０００ｒ
ｐｍで１０分間、遠心分離した。遠心分離後１０分間放
置し、その後血液成分採取用容器１０を観察した結果、
図１０（ｂ）に示したように赤血球４ａと血漿４ｂが分
離していた。The blood of the subject A was collected with a syringe, and 1 ml of the blood was collected in the cylindrical body 11 of the blood component collecting container 10 by piercing the stopper with an injection needle. Next, the blood component collection container 10 was subjected to 3000 r using a centrifuge (manufactured by Domestic Centrifuge Co., trade name: H-20).
Centrifuged at pm for 10 minutes. After centrifugation, the vessel was left for 10 minutes.
As shown in FIG. 10B, red blood cells 4a and plasma 4b were separated.
【００７１】保存安定性試験 実施例５の血漿を分離した容器を用いて、実施例１の保
存安定性試験と同様に操作した。得られた結果を表５に
示した。表５より、本発明の血液成分採取用容器を用い
たものでは、直後および７日後の測定値に変化がないこ
とがわかる。Storage stability test Using the container from which the plasma of Example 5 was separated, the same operation as in the storage stability test of Example 1 was performed. Table 5 shows the obtained results. Table 5 shows that in the case of using the blood component collection container of the present invention, there is no change in the measured values immediately after and 7 days later.
【００７２】[0072]
【表５】 [Table 5]
【００７３】（実施例６）実施例５で用意した血液成分
採取用容器において、管状容器２および筒状体１１内を
減圧し、図１１に示した血液成分採取用容器１０を作製
した。この場合、減圧にあたっては、真空打栓機（共和
真空社製、商品名：ＶＳ−１５０Ａ）を用い、０．８５
気圧で栓体６を打栓し、血液１ｍｌ真空採血し得るよう
に構成した。(Example 6) In the blood component collection container prepared in Example 5, the pressure inside the tubular container 2 and the cylindrical body 11 was reduced, and the blood component collection container 10 shown in FIG. 11 was produced. In this case, a vacuum stopper (Kyowa Vacuum Co., trade name: VS-150A) was used to reduce the pressure by 0.85.
The stopper 6 was stoppered at atmospheric pressure, and 1 ml of blood was collected under vacuum.
【００７４】血液成分採取用容器１０をマルチプル採血
針が取り付けられた真空採血用ホルダーにセットし、マ
ルチプル採血針を被験者Ａ氏の血管に刺通し、該採血針
の他端を栓体６に刺通して行う常法の真空採血法によ
り、血液成分採取用容器１０に血液を１ｍｌ採血した。
その後１０分間放置し、血液成分採取用容器１０を観察
した結果、図１２（ｂ）に示したように赤血球４ａと血
漿４ｂが分離していた。The blood component collecting container 10 is set in a vacuum blood collecting holder to which a multiple blood collecting needle is attached, and the multiple blood collecting needle is pierced into the blood vessel of the subject A, and the other end of the blood collecting needle is pierced into the stopper 6. 1 ml of blood was collected in the blood component collecting container 10 by a conventional vacuum blood sampling method.
After that, the container was left for 10 minutes, and the blood component collection container 10 was observed. As a result, the red blood cells 4a and the plasma 4b were separated as shown in FIG.
【００７５】保存安定性試験 実施例６の血漿を分離した容器を用いて、実施例１の保
存安定性試験と同様に操作した。得られた結果を表６に
示した。表６より、本発明の血液成分採取用容器を用い
たものでは、直後および７日後の測定値に変化がないこ
とがわかる。Storage stability test Using the container from which the plasma of Example 6 was separated, the same operation as in the storage stability test of Example 1 was performed. Table 6 shows the obtained results. Table 6 shows that in the case of using the blood component collection container of the present invention, there is no change in measured values immediately after and 7 days later.
【００７６】[0076]
【表６】 [Table 6]
【００７７】[0077]
【発明の効果】請求項１記載の発明にかかる血液成分採
取用容器では、筒状体内に血液を導入し、必要に応じて
遠心分離すると、血漿等と固形分とが接触しない状態と
なるので、分離状態のままで長期間保存しても検査値に
影響なく血漿等を保存できる。In the blood component collecting container according to the first aspect of the present invention, when blood is introduced into the cylindrical body and centrifuged as necessary, plasma and the like do not come into contact with solids. Even if stored for a long time in the separated state, plasma and the like can be stored without affecting test values.
【００７８】請求項２記載の発明にかかる血液成分採取
用容器では、筒状体内に血液を導入し、必要に応じて遠
心分離すると、血漿等と固形分とが接触しない状態とな
るので、分離状態のままで長期間保存しても検査値に影
響なく血漿等を保存できる。In the blood component collecting container according to the second aspect of the present invention, when blood is introduced into the cylindrical body and centrifuged as necessary, plasma and the like do not come into contact with solids. Even if stored for a long time in the state, plasma and the like can be stored without affecting the test values.
【００７９】請求項３記載の発明にかかる血液成分採取
用容器では、筒状体内に血液を導入し、必要に応じて遠
心分離すると、赤血球は袋状部に溜まり、袋状部の周囲
の親水性微粒子層上には溜まりにくいので、血漿の分離
性が低下しにくい。また、血漿と赤血球とが接触しない
状態となるので、分離状態のままで長期間保存しても検
査値に影響なく血漿を保存できる。In the blood component collecting container according to the third aspect of the present invention, when blood is introduced into the cylindrical body and centrifuged as necessary, red blood cells accumulate in the bag-like portion, and the red blood cells around the bag-like portion become hydrophilic. Since it does not easily accumulate on the porous fine particle layer, the separability of plasma does not easily decrease. In addition, since plasma and red blood cells do not come into contact with each other, plasma can be stored without affecting test values even when stored for a long time in the separated state.
【００８０】請求項４記載の発明にかかる血液成分採取
用容器では、筒状体内に血液を導入し、必要に応じて遠
心分離すると、多量の血液を分離しても、赤血球は袋状
部に溜まり、傾斜部には溜まりにくいので、血漿の分離
性が低下しにくい。また、血漿と赤血球とが接触しない
状態となるので、分離状態のままで長期間保存しても検
査値に影響なく血漿を保存できる。In the blood component collecting container according to the fourth aspect of the present invention, blood is introduced into a cylindrical body, and centrifuged as necessary, so that even if a large amount of blood is separated, erythrocytes remain in a bag-shaped portion. Since it is difficult to accumulate in pools and inclined portions, plasma separation properties are unlikely to decrease. In addition, since plasma and red blood cells do not come into contact with each other, plasma can be stored without affecting test values even when stored for a long time in the separated state.
【００８１】請求項５記載の発明にかかる血液成分採取
用容器では、従来の真空採血管の使用方法と同様に、マ
ルチプル注射針および採血ホルダーを用いて真空採血法
によって採血すると、管状容器および筒状体内が減圧に
されているので、減圧の程度に応じて血液を速やかに採
血できると同時に、上記筒状体内に採血された血液が速
やかに分離されて、筒状体内に固形分が残り、血漿等が
管状容器に溜まる。このように、血漿等と固形分とが接
触しない状態となるので、分離状態のままで長期間保存
しても検査値に影響なく血漿等を保存できる。In the blood component collecting container according to the fifth aspect of the present invention, in a manner similar to the conventional method of using a vacuum blood collection tube, when a blood is collected by a vacuum blood collection method using a multiple injection needle and a blood collection holder, a tubular container and a tube are obtained. Since the pressure in the body is reduced, the blood can be quickly collected according to the degree of the pressure reduction, and at the same time, the blood collected in the cylinder is quickly separated, and the solid content remains in the cylinder, Plasma and the like accumulate in the tubular container. As described above, since the plasma and the like do not come into contact with the solid content, the plasma and the like can be stored without affecting the test values even when stored in a separated state for a long period of time.
【図１】請求項１記載の発明にかかる血液成分採取用容
器の構造例を示す断面図。FIG. 1 is a cross-sectional view showing a structural example of a blood component collection container according to the first embodiment.
【図２】（ａ）および（ｂ）は、それぞれ、図１に示し
た血液成分採取用容器を用いた場合の血液成分分離工程
を説明するための各断面図。2 (a) and 2 (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 1 is used.
【図３】請求項５記載の発明にかかる血液成分採取用容
器の構造例を示す断面図。FIG. 3 is a sectional view showing a structural example of a blood component collecting container according to the invention of claim 5;
【図４】（ａ）および（ｂ）は、それぞれ、図３に示し
た血液成分採取用容器を用いた場合の血液成分分離工程
を説明するための各断面図。4 (a) and 4 (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 3 is used.
【図５】請求項２記載の発明にかかる血液成分採取用容
器の構造例を示す断面図。FIG. 5 is a cross-sectional view showing a structural example of a blood component collection container according to the invention of claim 2;
【図６】（ａ）および（ｂ）は、それぞれ、図５に示し
た血液成分採取用容器を用いた場合の血液成分分離工程
を説明するための各断面図。6 (a) and 6 (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 5 is used.
【図７】請求項５記載の発明にかかる血液成分採取用容
器の構造例を示す断面図。FIG. 7 is a sectional view showing a structural example of a blood component collecting container according to the invention of claim 5;
【図８】（ａ）および（ｂ）は、それぞれ、図７に示し
た血液成分採取用容器を用いた場合の血液成分分離工程
を説明するための各断面図。8 (a) and (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 7 is used.
【図９】請求項３記載の発明にかかる血液成分採取用容
器の構造例を示す断面図。FIG. 9 is a cross-sectional view showing a structural example of a blood component collection container according to the third embodiment.
【図１０】（ａ）および（ｂ）は、それぞれ、図９に示
した血液成分採取用容器を用いた場合の血液成分分離工
程を説明するための各断面図。FIGS. 10 (a) and (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 9 is used.
【図１１】請求項５記載の発明にかかる血液成分採取用
容器の構造例を示す断面図。FIG. 11 is a sectional view showing a structural example of a blood component collecting container according to the invention of claim 5;
【図１２】（ａ）および（ｂ）は、それぞれ、図１１に
示した血液成分採取用容器を用いた場合の血液成分分離
工程を説明するための各断面図。12 (a) and (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 11 is used.
【図１３】請求項４記載の発明にかかる血液成分採取用
容器の構造例を示す断面図。FIG. 13 is a sectional view showing a structural example of a blood component collecting container according to the invention of claim 4.
【図１４】（ａ）および（ｂ）は、それぞれ、図１３に
示した血液成分採取用容器を用いた場合の血液成分分離
工程を説明するための各断面図。FIGS. 14 (a) and (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 13 is used.
【図１５】請求項５記載の発明にかかる血液成分採取用
容器の構造例を示す断面図。FIG. 15 is a cross-sectional view showing a structural example of a blood component collecting container according to the invention of claim 5;
【図１６】（ａ）および（ｂ）は、それぞれ、図１５に
示した血液成分採取用容器を用いた場合の血液成分分離
工程を説明するための各断面図。FIGS. 16 (a) and (b) are cross-sectional views for explaining a blood component separation step when the blood component collection container shown in FIG. 15 is used.
１、５、７、１０、１３ 血液成分採取用容器 ２ 管状容器 ２ａ 上端開口 ３、８、１１、１４ 筒状体 ３ａ、８ａ、１１ａ 底部 ４ 血液 ４ａ 固形分（赤血球） ４ｂ 血漿等 ６ 栓体 ９ 親水性微粒子層 １２ 袋状部 １２ａ 開口 １５ 傾斜部 １６ 袋状部 1, 5, 7, 10, 13 Blood component collection container 2 Tubular container 2a Upper end opening 3, 8, 11, 14 Cylindrical body 3a, 8a, 11a Bottom part 4 Blood 4a Solid content (red blood cells) 4b Plasma etc. 6 Plug 9 hydrophilic fine particle layer 12 bag-shaped part 12a opening 15 inclined part 16 bag-shaped part
Claims (5)
た有底の筒状体とからなり、 該筒状体の底部は直径０．１〜１０μｍの複数の孔を有
する材料から構成されると共に液体透過性であることを
特徴とする血液成分採取用容器。1. A bottomed tubular container and a bottomed tubular body disposed in the container, wherein the bottom of the tubular body is made of a material having a plurality of holes having a diameter of 0.1 to 10 μm. A container for collecting blood components, which is constituted and is liquid-permeable.
た有底の筒状体とからなり、 該筒状体の底部は液体透過性であり、 該筒状体の内部下方に直径０．１〜２００μｍの親水性
微粒子層が存在していることを特徴とする血液成分採取
用容器。2. A tubular container having a bottom and a bottomed tubular body disposed in the container, wherein the bottom of the tubular body is liquid-permeable, A container for collecting blood components, wherein a hydrophilic fine particle layer having a diameter of 0.1 to 200 μm is present.
有する赤血球不透過性の袋状部がその開口を残して埋め
込まれると共に、上記親水性微粒子層の上面が該袋状部
の開口に向かって傾斜していることを特徴とする請求項
２記載の血液成分採取用容器。3. An erythrocyte-impermeable bag-like portion having an opening at an upper end is embedded in the hydrophilic fine-particle layer while leaving the opening, and the upper surface of the hydrophilic fine-particle layer is opened at the opening of the bag-like portion. 3. The container for collecting blood components according to claim 2, wherein the container is inclined toward.
た有底の筒状体とからなり、 該筒状体の底部は、底部先端に向かって傾斜した傾斜部
と該傾斜部に連設する袋状部とから構成され、 該傾斜部は直径０．１〜１０μｍの複数の孔を有する材
料が用いられ液体透過性であり、 該袋状部は赤血球不透過性であることを特徴とする血液
成分採取用容器。4. A bottomed tubular container and a bottomed tubular body disposed in the container, wherein the bottom of the tubular body has an inclined portion inclined toward a bottom tip and the inclined portion. The inclined portion is made of a material having a plurality of holes having a diameter of 0.1 to 10 μm and is liquid-permeable, and the bag-shaped portion is impermeable to red blood cells. A blood component collecting container characterized by the above-mentioned.
に取り付けられた栓体を更に備え、上記管状容器および
上記筒状体内が減圧にされていることを特徴とする請求
項１〜４のいずれか１項に記載の血液成分採取用容器。5. The apparatus according to claim 1, further comprising a stopper attached so as to close an upper end opening of said tubular container, wherein said tubular container and said tubular body are evacuated. The blood component collection container according to any one of the above items.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10000979A JPH11192216A (en) | 1998-01-06 | 1998-01-06 | Blood component drawing container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10000979A JPH11192216A (en) | 1998-01-06 | 1998-01-06 | Blood component drawing container |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11192216A true JPH11192216A (en) | 1999-07-21 |
Family
ID=11488738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10000979A Pending JPH11192216A (en) | 1998-01-06 | 1998-01-06 | Blood component drawing container |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH11192216A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009544968A (en) * | 2006-07-21 | 2009-12-17 | ベクトン・ディキンソン・アンド・カンパニー | Double layer tube with membrane for sample collection |
| JP2011033434A (en) * | 2009-07-31 | 2011-02-17 | Kao Corp | Trace solid sample analysis instrument |
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|---|---|---|---|---|
| US3682596A (en) * | 1967-10-23 | 1972-08-08 | Schering Corp | Body fluid collector and separator having improved flow rate |
| JPH07167858A (en) * | 1993-09-14 | 1995-07-04 | Becton Dickinson & Co | Blood-gathering assembly including insert made of coagulation promoting plastic |
| JPH11239574A (en) * | 1997-12-24 | 1999-09-07 | I Design:Kk | Blood drawing tube |
| JP2000074910A (en) * | 1997-09-16 | 2000-03-14 | Sekisui Chem Co Ltd | Container for blood test and blood test method |
-
1998
- 1998-01-06 JP JP10000979A patent/JPH11192216A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3682596A (en) * | 1967-10-23 | 1972-08-08 | Schering Corp | Body fluid collector and separator having improved flow rate |
| JPH07167858A (en) * | 1993-09-14 | 1995-07-04 | Becton Dickinson & Co | Blood-gathering assembly including insert made of coagulation promoting plastic |
| JP2000074910A (en) * | 1997-09-16 | 2000-03-14 | Sekisui Chem Co Ltd | Container for blood test and blood test method |
| JPH11239574A (en) * | 1997-12-24 | 1999-09-07 | I Design:Kk | Blood drawing tube |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009544968A (en) * | 2006-07-21 | 2009-12-17 | ベクトン・ディキンソン・アンド・カンパニー | Double layer tube with membrane for sample collection |
| JP2011033434A (en) * | 2009-07-31 | 2011-02-17 | Kao Corp | Trace solid sample analysis instrument |
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