JPH11137285A - Production of ribitol - Google Patents
Production of ribitolInfo
- Publication number
- JPH11137285A JPH11137285A JP9329695A JP32969597A JPH11137285A JP H11137285 A JPH11137285 A JP H11137285A JP 9329695 A JP9329695 A JP 9329695A JP 32969597 A JP32969597 A JP 32969597A JP H11137285 A JPH11137285 A JP H11137285A
- Authority
- JP
- Japan
- Prior art keywords
- ribitol
- culture
- trichosporonoides
- valve
- exchange resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- JVWLUVNSQYXYBE-UHFFFAOYSA-N Ribitol Natural products OCC(C)C(O)C(O)CO JVWLUVNSQYXYBE-UHFFFAOYSA-N 0.000 title claims abstract description 62
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 title claims abstract description 62
- HEBKCHPVOIAQTA-ZXFHETKHSA-N ribitol Chemical compound OC[C@H](O)[C@H](O)[C@H](O)CO HEBKCHPVOIAQTA-ZXFHETKHSA-N 0.000 title claims abstract description 62
- 238000004519 manufacturing process Methods 0.000 title claims description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 29
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 241000908249 Trichosporonoides Species 0.000 claims abstract description 18
- 239000003729 cation exchange resin Substances 0.000 claims abstract description 18
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 15
- 244000005700 microbiome Species 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 8
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 229910052782 aluminium Inorganic materials 0.000 claims abstract description 5
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims abstract description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000004587 chromatography analysis Methods 0.000 claims abstract 2
- 230000002378 acidificating effect Effects 0.000 claims description 8
- 238000005194 fractionation Methods 0.000 abstract description 15
- 239000002994 raw material Substances 0.000 abstract description 10
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 abstract description 5
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 abstract description 5
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 abstract description 5
- 239000001963 growth medium Substances 0.000 abstract description 4
- 229910052742 iron Inorganic materials 0.000 abstract description 3
- 241000908253 Moniliella oedocephalis Species 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract 1
- 229910052748 manganese Inorganic materials 0.000 abstract 1
- 239000011572 manganese Substances 0.000 abstract 1
- 235000000346 sugar Nutrition 0.000 description 13
- 239000007788 liquid Substances 0.000 description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 9
- 239000008103 glucose Substances 0.000 description 9
- 239000013078 crystal Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- 238000004811 liquid chromatography Methods 0.000 description 6
- 239000004386 Erythritol Substances 0.000 description 5
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 5
- 235000019414 erythritol Nutrition 0.000 description 5
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 5
- 229940009714 erythritol Drugs 0.000 description 5
- 235000011187 glycerol Nutrition 0.000 description 5
- 229930091371 Fructose Natural products 0.000 description 4
- 239000005715 Fructose Substances 0.000 description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 238000002425 crystallisation Methods 0.000 description 4
- 230000008025 crystallization Effects 0.000 description 4
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 239000003957 anion exchange resin Substances 0.000 description 3
- 239000002518 antifoaming agent Substances 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- -1 yeast extract Chemical class 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical group [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
- 125000000962 organic group Chemical group 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- 241000206506 Adonis vernalis Species 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 241000202722 Bupleurum falcatum Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 101001116774 Homo sapiens Methionine-R-sulfoxide reductase B2, mitochondrial Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 description 1
- 102100024862 Methionine-R-sulfoxide reductase B2, mitochondrial Human genes 0.000 description 1
- 241001182779 Moniliella megachiliensis Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 241000191472 Yamadazyma triangularis Species 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- LFYJSSARVMHQJB-QIXNEVBVSA-N bakuchiol Chemical compound CC(C)=CCC[C@@](C)(C=C)\C=C\C1=CC=C(O)C=C1 LFYJSSARVMHQJB-QIXNEVBVSA-N 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002213 purine nucleotide Substances 0.000 description 1
- 125000000561 purinyl group Chemical group N1=C(N=C2N=CNC2=C1)* 0.000 description 1
- 239000011949 solid catalyst Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 125000000542 sulfonic acid group Chemical group 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【０００１】[0001]
【０００２】本発明は、リビトールの製造方法に関し、
更に詳しくは、微生物を利用して醗酵法により工業的に
有利にリビトールを製造する方法に関する。[0002] The present invention relates to a method for producing ribitol,
More particularly, the present invention relates to a method for industrially advantageously producing ribitol by a fermentation method using a microorganism.
【０００３】[0003]
【０００４】リビトールは、洋種フクジュソウのAdonis
vernalis L.（キンポウゲ科）およびミシマサイコBupl
eurum falcatum L.（セリ科）の根に含まれる５単糖ア
ルコールの一種である。[0004] Ribitol is a Western species of Adonis
vernalis L. (Ranunculaceae) and Mishimasaiko Bupl
eurum falcatum is a kind of pentasaccharide alcohol contained in the root of L. (Apiaceae).
【０００５】このリビトールは、リボースを各種固体触
媒の存在下で接触水素還元することで得る事が出来る
が、その他にも、キャンジダ・ポリモルファ或いはトル
ロプシス・ハマダをリボースに接種し、醗酵する方法
（特公昭４５−２０７１号）、リボースを主炭素源とす
る培地でコリネバクテリウム（Corynebacterium）に属
する菌株を培養する方法（特公昭４９−１２７１８号）
等で製造する事ができる。[0005] This ribitol can be obtained by catalytic hydrogen reduction of ribose in the presence of various solid catalysts. Alternatively, ribitol can be obtained by inoculating Candida polymorpha or Torulopsis hamada to ribose and fermenting it. No. 45-2071), a method of culturing a strain belonging to Corynebacterium in a medium containing ribose as a main carbon source (Japanese Patent Publication No. 49-12718).
Etc. can be manufactured.
【０００６】しかし、原料として使用されるリボースは
主としてプリンヌクレオチドの化学分解により製造され
るが、高価であり、実際の工業的なリビトールの製造原
料としては適切ではない。[0006] However, ribose used as a raw material is produced mainly by chemical decomposition of purine nucleotides, but is expensive and is not suitable as a practical industrial raw material for producing ribitol.
【０００７】この他にも、光合成を行う藻類に光線を照
射しながら培養することでリビトールを生成する方法
（特公昭４４−１６３５０号）が報告されているが、リ
ビトールが培養液中に蓄積するまでには長時間を必要と
し、その蓄積量も少なく、従って必ずしも満足のいく方
法ではなかった。[0007] In addition, there has been reported a method of producing ribitol by irradiating algae for photosynthesis with irradiation of light (Japanese Patent Publication No. 44-16350). However, ribitol is accumulated in a culture solution. By the time, a long time was required, the amount of accumulation was small, and therefore, it was not always a satisfactory method.
【０００８】本発明は、入手が容易で且つ安価な原料を
用いて、工業的にリビトールを製造する方法を見出すこ
とを目的としている。An object of the present invention is to find a method for industrially producing ribitol using readily available and inexpensive raw materials.
【０００９】[0009]
【００１０】本発明者等は、前記課題を解決するために
鋭意検討した結果、トリコスポロノイデス属に属する微
生物を、グルコース、フラクトース或いは蔗糖などの醗
酵性糖質を主炭素源とする培地で培養することでリビト
ールが生成し、更に、特定の培養条件ではリビトールが
高収率で生成することを見出し、本発明を完成するに至
った。The present inventors have conducted intensive studies to solve the above-mentioned problems. As a result, the present inventors have found that microorganisms belonging to the genus Trichosporonoides can be cultured in a medium containing fermentable sugars such as glucose, fructose or sucrose as a main carbon source. It has been found that culturing produces ribitol, and furthermore, it has been found that ribitol is produced at a high yield under specific culturing conditions, thereby completing the present invention.
【００１１】即ち、本発明の課題を解決するための手段
は、下記の通りである。That is, means for solving the problems of the present invention are as follows.
【００１２】第１に、トリコスポロノイデス属に属する
微生物を、醗酵性糖質を主炭素源とする培地で培養し、
得られた培養物からリビトールを採取することを特徴と
するリビトールの製造方法。第２に、トリコスポロノイ
デス属に属する微生物が、トリコスポロノイデス・オエ
ドセファリスである上記第１に記載のリビトールの製造
方法。第３に、培養が、糖質濃度１０〜４５重量％、ｐ
Ｈ３〜５．５、温度２４〜３８℃の範囲内で、好気的条
件で実施される上記第１または２に記載のリビトールの
製造方法。第４に、培養物からのリビトールの採取が、
培養物を陽イオン交換樹脂を充填した塔に供給してリビ
トール画分を分離するクロマト分画である上記第１〜３
の何れか一つに記載のリビトールの製造方法。第５に、
陽イオン交換樹脂が、アルミニウム形、マンガン形また
は鉄形の何れかの強酸性陽イオン交換樹脂である上記第
４に記載のリビトールの製造方法。First, a microorganism belonging to the genus Trichosporonoides is cultured in a medium containing fermentable carbohydrate as a main carbon source.
A method for producing ribitol, comprising collecting ribitol from the obtained culture. Secondly, the method for producing ribitol according to the first aspect, wherein the microorganism belonging to the genus Trichosporonoides is Trichosporonoides oedespharis. Third, the cultures have carbohydrate concentrations of 10-45% by weight, p
The method for producing ribitol according to the first or second aspect, wherein the method is performed under aerobic conditions at a temperature of H3 to 5.5 and a temperature of 24 to 38 ° C. Fourth, harvesting ribitol from the culture
The first to third chromatographic fractions, in which the culture is supplied to a column filled with a cation exchange resin to separate a ribitol fraction,
The method for producing ribitol according to any one of the above. Fifth,
The method for producing ribitol according to the above item 4, wherein the cation exchange resin is a strongly acidic cation exchange resin in any of an aluminum form, a manganese form and an iron form.
【００１３】本発明において用いられる微生物は、トリ
コスポロノイデス（Trichosporonoides）属に属し、且
つグルコース、フラクトース或いは蔗糖などの醗酵性糖
質からリビトールを生産する能力を有する微生物であれ
ば特に制限はない。The microorganism used in the present invention is not particularly limited as long as it belongs to the genus Trichosporonoides and has the ability to produce ribitol from fermentable sugars such as glucose, fructose or sucrose. .
【００１４】トリコスポロノイデス属に属する微生物と
しては、例えば、トリコスポロノイデス・オエドセファ
リス（Trichosporonoides oedocephalis）、トリコスポ
ロノイデス・メガキリエンシス（Trichosporonoides me
gachiliensis）等を上げることができ、特に、トリコス
ポロノイデス・オエドセファリスがリビトールの製造に
最も適している。Examples of microorganisms belonging to the genus Trichosporonoides include, for example, Trichosporonoides oedocephalis and Trichosporonoides megachiriensis (Trichosporonoides me).
gachiliensis), and in particular, Trichosporonoides oeedespharis is most suitable for producing ribitol.
【００１５】これら微生物の菌株はオランダ国BaarnのC
entral Bureau voor Schimmelcultures(CBS)や米国Mary
landのAmerican Type Culture Collection(ATCC)より容
易に入手可能であり、具体的には、例えばトリコスポロ
ノイデス・オエドセファリスは米国のＡＴＣＣより“Ａ
ＴＣＣ １６９５８”として、またトリコスポロノイデ
ス・メガキリエンシスはオランダ国のＣＢＳより“ＣＢ
Ｓ １９１．９２”として入手できる。The strains of these microorganisms are C
entral Bureau voor Schimmelcultures (CBS) and Mary, USA
land is readily available from the American Type Culture Collection (ATCC); specifically, for example, Trichosporonoides oedespharis is available from the ATCC in the United States as "A
TCC 16958 ”and Trichosporonoides megachiriensis from the Dutch CBS“ CB
S 191.92 ".
【００１６】本発明の炭素源としては、グルコース、フ
ラクトース、蔗糖等の糖質が採用できるが、グルコース
を炭素源とした場合がリビトールを生産する為の培養時
間が短く、更にリビトールへの転化率も高く好ましい。
これらの炭素源は、通常単独で使用されるが、２種以上
混合しても使用することができる。As the carbon source of the present invention, carbohydrates such as glucose, fructose and sucrose can be employed. When glucose is used as the carbon source, the cultivation time for producing ribitol is short, and the conversion to ribitol is further reduced. Is also preferred.
These carbon sources are usually used alone, but may be used in combination of two or more.
【００１７】炭素源としての糖の濃度は、１０〜４５重
量％、好ましくは３０〜４０重量％で実施される。The concentration of sugar as a carbon source is 10 to 45% by weight, preferably 30 to 40% by weight.
【００１８】炭素源である糖の濃度が１０重量％より低
い場合には、醗酵後の精製工程での取扱い量が増え、大
きな処理設備が必要となり、更に精製した液の濃縮に多
くのエネルギーが必要となり、工業的に不利である。When the concentration of the sugar as a carbon source is lower than 10% by weight, the amount of handling in the purification step after fermentation increases, a large treatment facility is required, and more energy is required for concentrating the purified liquid. Required and industrially disadvantageous.
【００１９】また、炭素源である糖の濃度が４５重量％
より高い場合には、糖が全て消費されるまでに多くの時
間がかかり、好ましくない。The concentration of sugar as a carbon source is 45% by weight.
If it is higher, it takes much time until all the sugar is consumed, which is not preferable.
【００２０】本発明での培養は、好気的条件で液体培地
を用いて実施され、特に通気撹拌で実施するのが好まし
い。The cultivation in the present invention is carried out under aerobic conditions using a liquid medium, and is particularly preferably carried out with aeration and agitation.
【００２１】培養時間は、培地の種類及び炭素源である
糖の濃度によっても異なるが、通常、５〜１５日間程度
であり、例えば炭素源としての糖が全て消費されるまで
かリビトールの生成量が最大となった時点で培養を停止
する。The cultivation time varies depending on the type of medium and the concentration of sugar as a carbon source, but is usually about 5 to 15 days, for example, until all of the sugar as a carbon source is consumed or the production amount of ribitol. The culture is stopped at the time when is maximum.
【００２２】培養液中の炭素源としての糖及びリビトー
ルは、高速液体クロマトグラフィーにより容易にその量
を分析することができる。The amounts of sugar and ribitol as a carbon source in the culture solution can be easily analyzed by high performance liquid chromatography.
【００２３】本発明の培養は、ｐＨを３〜５．５の範囲
に調整して実施される。The cultivation of the present invention is carried out by adjusting the pH to the range of 3 to 5.5.
【００２４】本発明の培養は、微生物が生育できる温度
範囲内、即ち２４〜３８℃で実施される。The cultivation of the present invention is carried out within a temperature range in which microorganisms can grow, that is, at 24 to 38 ° C.
【００２５】窒素源としては、微生物により利用可能な
酵母エキス、コーンスチープリカー、尿素等の窒素化合
物が用いられる。As the nitrogen source, there can be used nitrogen compounds such as yeast extract, corn steep liquor and urea which can be used by microorganisms.
【００２６】この他にも、必要により培養液には各種有
機物、無機物、消泡剤等を添加することができる。In addition, various organic substances, inorganic substances, antifoaming agents and the like can be added to the culture solution, if necessary.
【００２７】培養液中に生成したリビトールは、遠心分
離、濾過等により培養液から菌体を除去し、更に活性炭
処理やイオン交換樹脂等を使用して精製することによ
り、リビトール液として採取することができる。The ribitol produced in the culture solution is collected as a ribitol solution by removing the cells from the culture solution by centrifugation, filtration, etc., and further purifying using activated carbon treatment or an ion exchange resin. Can be.
【００２８】得られたリビトール液は、そのまま濃縮後
結晶化するか、または陽イオン交換樹脂を充填した充填
塔に通液し、各成分の樹脂に対する吸着性の差を利用し
たクロマト分画をすることでリビトールの含量を高めた
後に結晶化することでリビトール結晶を得ることができ
る。The resulting ribitol solution is concentrated as it is and then crystallized, or passed through a packed column filled with a cation exchange resin, and subjected to chromatographic fractionation utilizing the difference in the adsorptivity of each component to the resin. By increasing the content of ribitol and then crystallization, ribitol crystals can be obtained.
【００２９】本発明のクロマト分画で使用される陽イオ
ン交換樹脂は、市販のほとんどの樹脂が採用可能だが、
中でもスチレン−ジビニルベンゼンの架橋重合体にスル
ホン酸基が結合した強酸性陽イオン交換樹脂が市販され
ているので、これに常法によりアルミニウム、マグネシ
ウム、バリウム、マンガン、鉄、カリウム等のイオンを
チャージしたものが採用でき、特にアルミニウムイオ
ン、マンガンイオンまたは鉄イオンの何れかを強酸性陽
イオン交換樹脂にチャージしたアルミニウム形、マンガ
ン形または鉄形強酸性陽イオン交換樹脂がリビトールを
培養液中の他の成分から分離するのにすぐれている。As the cation exchange resin used in the chromatographic fractionation of the present invention, most commercially available resins can be used.
Among them, a strongly acidic cation exchange resin in which a sulfonic acid group is bonded to a crosslinked polymer of styrene-divinylbenzene is commercially available, and charged with ions such as aluminum, magnesium, barium, manganese, iron, and potassium by a conventional method. In particular, aluminum, manganese, or iron-type strongly acidic cation exchange resin charged with aluminum ion, manganese ion, or iron ion to the strongly acidic cation exchange resin can be used to separate ribitol from the culture medium. Excellent for separating from the components of
【００３０】また、本発明のクロマト分画は、回分式ま
たは擬似移動床式、単塔式または多塔式の何れもが採用
可能である。The chromatographic fractionation of the present invention can be any of a batch type or a simulated moving bed type, a single column type or a multiple column type.
【００３１】[0031]
【００３２】以下に実施例をあげて更に具体的に本発明
の方法を説明するが、本発明の技術的範囲は以下の例に
制限されるものではない。また、以下の実施例におい
て、％は特に断らない限り重量％を表わすものとする。Hereinafter, the method of the present invention will be described more specifically with reference to examples, but the technical scope of the present invention is not limited to the following examples. In the following examples,% represents% by weight unless otherwise specified.
【００３３】[0033]
【実施例１】Embodiment 1
【００３４】（前培養）無水結晶ブドウ糖２００ｇ／リ
ットル及びコーンスチープリカー１５ｇ／リットルを含
む培地１００ｍｌを５００ｍｌの坂口フラスコに入れ、
トリコスポロノイデス・オエドセファリス（ＡＴＣＣ
１６９５８）の１白金耳を接種し、３５℃で２日間振と
う培養を行い、前培養液を得た。(Preculture) 100 ml of a medium containing 200 g / l of anhydrous crystalline glucose and 15 g / l of corn steep liquor was placed in a 500 ml Sakaguchi flask.
Trichosporonoides Oedes Separis (ATCC
16958) was inoculated and shake-cultured at 35 ° C. for 2 days to obtain a pre-culture solution.
【００３５】（本培養）無水結晶ブドウ糖３ｋｇ、コー
ンスチープリカー２２５ｇ、前培養液６００ｍｌ（前培
養液６本）及び消泡剤４．５ｇ（日本油脂（株）製、デ
ィスホーム ＣＡ−１２３）を含む培地１５リットルを
３０リットルの培養器に入れ、ｐＨ４．５、温度３５
℃、回転数８００ｒｐｍ、通気量１．０ｖｖｍで８日間
培養を行った。この時、培養液中のグルコースは全て消
費されていた。培養液は、液体クロマトグラフィーを用
いて分析したところ、培養液１ｍｌ当たりリビトール５
７．４ｍｇ（収率２８．７％）、エリスリトール３８．
６ｍｇ（収率１９．３％）、グリセリン４．６ｍｇ（収
率２．３％）を含有していた。次に遠心分離器を用いて
この培養液から菌体を分離した後、２０ｇの活性炭（武
田薬品（株）製、白鷺）とともに温度５０℃で１時間撹
拌後、活性炭を濾別し、濾液はカチオン交換樹脂（オル
ガノ（株）製、ＩＲ−１２０Ｂ）３００ｍｌ及びアニオ
ン交換樹脂（オルガノ（株）製、ＩＲＡ−４１０）５０
０ｍｌを充填したカラムに通液した後、濃度６０％まで
濃縮し、リビトール含有液２５１０ｇを得た。(Main culture) 3 kg of anhydrous crystalline glucose, 225 g of corn steep liquor, 600 ml of preculture liquid (six preculture liquids), and 4.5 g of antifoaming agent (Nippon Oil & Fats Co., Ltd., Deform CA-123) 15 liters of the culture medium into a 30 liter incubator, pH 4.5, temperature 35
Culturing was performed for 8 days at a temperature of 800 ° C., a rotation speed of 800 rpm, and an aeration rate of 1.0 vvm. At this time, all the glucose in the culture solution had been consumed. The culture solution was analyzed using liquid chromatography, and it was found that 5 ml of ribitol per 1 ml of the culture solution was used.
7.4 mg (28.7% yield), erythritol
It contained 6 mg (19.3% yield) and 4.6 mg glycerin (2.3% yield). Next, the cells were separated from the culture using a centrifugal separator, stirred with 50 g of activated carbon (manufactured by Takeda Pharmaceutical Co., Ltd., Shirasagi) at a temperature of 50 ° C. for 1 hour, and the activated carbon was filtered off. 300 ml of cation exchange resin (IR-120B, manufactured by Organo Co., Ltd.) and 50 anion exchange resin (IRA-410, manufactured by Organo Co., Ltd.)
After passing through a column filled with 0 ml, the solution was concentrated to a concentration of 60% to obtain 2510 g of a ribitol-containing solution.
【００３６】（クロマト分画装置）本実施例で使用した
クロマト分画装置は、図１の概略図に示すように、１リ
ットルのジャケット付きガラス製の塔（内径３．６ｃ
ｍ、長さ１０５ｃｍ）６本Ａ〜Ｆを直列に連結し、塔Ａ
の上部に予熱器Ｋ及びバルブＨを介して原料仕込みポン
プＧを接続するとともに、予熱器Ｋ及びバルブＪを介し
て水仕込みポンプＩを接続したものである。また、該ク
ロマト分画装置は、塔Ｆの下部に、バルブＮ及びＯを介
して、流出液受けタンクＬ及びＭを取り付けると共に、
バルブＯより先端側にバルブＰを取り付けたものであ
る。尚、塔Ａ〜Ｆの各塔には、強酸性陽イオン交換樹脂
（オルガノ（株）製、ＣＲ−１３１０）のアルミニウム
形を塔１本当たり１０００ｍｌ充填した。(Chromatographic Fractionation Apparatus) As shown in the schematic diagram of FIG. 1, the chromatographic fractionation apparatus used in this example is a 1-liter jacketed glass tower (with an inner diameter of 3.6 c).
m, 105cm in length) A to F are connected in series,
Is connected to a raw material charging pump G via a preheater K and a valve H, and to a water charging pump I via a preheater K and a valve J. In addition, the chromatographic fractionation apparatus attaches effluent receiving tanks L and M to the lower part of the column F via valves N and O,
The valve P is attached to the tip side of the valve O. Each of the towers A to F was filled with 1000 ml of an aluminum form of a strongly acidic cation exchange resin (CR-1310, manufactured by Organo Corporation) per one tower.
【００３７】（クロマト分画）Ａ〜Ｆの各塔を６０℃に
保ちつつ、バルブＨ及びＰを開き、バルブＪ、Ｎ及びＯ
を閉じた状態で、前記濃度６０％のリビトール含有液３
００ｍｌを毎分５０ｍｌの速さで流した。次にバルブＨ
を閉じ、バルブＪを開き、ポンプＩから水を毎時５０ｍ
ｌの速さで供給した。バルブＰの出口の濃度が０．２％
になった時点でバルブＰを閉じ、バルブＮを開いた。２
２分後、バルブＮを閉じ、バルブＯを開いた。バルブＯ
の出口の濃度が０．２％以下になった時点でバルブＯを
閉じ、バルブＰを開いた。この操作を繰り返した。タン
クＬ及びタンクＭに得られた流出液の濃度、重量及び糖
組成は、表１の通りであった。(Chromatographic fractionation) While maintaining the columns A to F at 60 ° C., the valves H and P were opened, and the valves J, N and O
Is closed, and the above-mentioned 60% concentration of ribitol-containing liquid 3
00 ml was flowed at a rate of 50 ml per minute. Next, valve H
Is closed, valve J is opened, and water is pumped 50 m / h from pump I.
Feed at a rate of 1. The concentration at the outlet of valve P is 0.2%
At this time, the valve P was closed and the valve N was opened. 2
Two minutes later, valve N was closed and valve O was opened. Valve O
When the concentration at the outlet of became 0.2% or less, the valve O was closed and the valve P was opened. This operation was repeated. Table 1 shows the concentrations, weights, and sugar compositions of the effluents obtained in the tanks L and M.
【００３８】[0038]
【表１】 [Table 1]
【００３９】（結晶化）タンクＬの流出液を濃度７７％
まで濃縮し、容量５００ｍｌの結晶化装置へ移し、温度
７０℃から１０時間かけて室温まで冷却した。途中６０
℃にて少量の結晶リビトール粉末をシードとして加え
た。生成した結晶は遠心分離器を用いて分離し、少量の
水で水洗した。得られた結晶は、乾燥後、１４４ｇであ
った。この結晶は、融点が１０２．８℃、液体クロマト
グラフィー分析で純度が９９．９％であり、液体クロマ
トグラフィー、ガスクロマトグラフィー、赤外線吸収ス
ペクトル及びＮＭＲスペクトルの分析結果から、リビト
ールであることを確認した。(Crystallization) The effluent of the tank L is made to have a concentration of 77%.
The solution was transferred to a crystallizer having a capacity of 500 ml, and cooled from room temperature to 70 ° C. over 10 hours. On the way 60
At 0 ° C., a small amount of crystalline ribitol powder was added as a seed. The generated crystals were separated using a centrifuge and washed with a small amount of water. The obtained crystals weighed 144 g after drying. This crystal had a melting point of 102.8 ° C., a purity of 99.9% by liquid chromatography analysis, and was confirmed to be ribitol by analysis of liquid chromatography, gas chromatography, infrared absorption spectrum and NMR spectrum. did.
【００４０】[0040]
【実施例２】Embodiment 2
【００４１】（本培養）無水結晶ブドウ糖１．５ｋｇ、
コーンスチープリカー１１２ｇ、実施例１の（前培養）
と同様にして製造した前培養液６００ｍｌ及び消泡剤
４．５ｇ（日本油脂（株）製、ディスホーム ＣＡ−１
２３）を含む培地１５リットルを３０リットルの培養器
に入れ、ｐＨ４．５、温度３５℃、回転数８００ｒｐ
ｍ、通気量０．８ｖｖｍで８日間培養を行った。この
時、培養液中のグルコースは全て消費されていた。培養
液は、液体クロマトグラフィーを用いて分析したとこ
ろ、培養液１ｍｌ当たりリビトール２９．５ｍｇ（収率
２９．５％）、エリスリトール１７．４ｍｇ（収率１
７．４％）、グリセリン１．７ｍｇ（収率１．７％）を
含有していた。次に遠心分離器を用いてこの培養液から
菌体を分離した後、２０ｇの活性炭（武田薬品（株）
製、白鷺）とともに温度５０℃で１時間撹拌後、活性炭
を濾別し、濾液はカチオン交換樹脂（オルガノ（株）
製、ＩＲ−１２０Ｂ）２００ｍｌ及びアニオン交換樹脂
（オルガノ（株）製、ＩＲＡ−４１０）３００ｍｌを充
填したカラムに通液した後、濃度６０％まで濃縮し、リ
ビトール含有液１２０５ｇを得た。(Main culture) 1.5 kg of anhydrous crystalline glucose,
Corn steep liquor 112g, Example 1 (pre-culture)
600 ml of a pre-culture liquid and 4.5 g of an antifoaming agent (manufactured by NOF CORPORATION, Deform CA-1)
15 liters of the medium containing 23) was placed in a 30 liter incubator, and the pH was 4.5, the temperature was 35 ° C., and the rotation speed was 800 rpm.
Culture was carried out for 8 days at a flow rate of 0.8 vvm. At this time, all the glucose in the culture solution had been consumed. The culture solution was analyzed using liquid chromatography. As a result, 29.5 mg of ribitol (yield 29.5%) and 17.4 mg of erythritol (1% yield) per ml of the culture solution were analyzed.
7.4%) and 1.7 mg of glycerin (1.7% yield). Next, cells were separated from the culture solution using a centrifugal separator, and then 20 g of activated carbon (Takeda Pharmaceutical Co., Ltd.)
After stirring for 1 hour at a temperature of 50 ° C. with Shirasagi), the activated carbon is filtered off, and the filtrate is a cation exchange resin (Organo Co., Ltd.)
(IRA-120B) and 300 ml of an anion exchange resin (IRA-410, manufactured by Organo Corporation), and then concentrated to a concentration of 60% to obtain 1205 g of a liquid containing ribitol.
【００４２】（クロマト分画）強酸性陽イオン交換樹脂
がマンガン形である以外は、実施例１と同様のクロマト
分画装置を使用して、以下のクロマト分画を実施した。
Ａ〜Ｆの各塔を６０℃に保ちつつ、バルブＨ及びＰを開
き、バルブＪ、Ｎ及びＯを閉じた状態で、前記濃度６０
％のリビトール含有液３００ｍｌを毎分５０ｍｌの速さ
で流した。次にバルブＨを閉じ、バルブＪを開き、ポン
プＩから水を毎時５０ｍｌの速さで供給した。バルブＰ
の出口の濃度が０．２％になった時点でバルブＰを閉
じ、バルブＮを開いた。２０分後、バルブＮを閉じ、バ
ルブＯを開いた。バルブＯの出口の濃度が０．２％以下
になった時点でバルブＯを閉じ、バルブＰを開いた。こ
の操作を繰り返した。タンクＬ及びタンクＭに得られた
流出液の濃度、重量及び糖組成は、表２の通りであっ
た。(Chromatographic fractionation) The following chromatographic fractionation was carried out using the same chromatographic fractionation apparatus as in Example 1 except that the strongly acidic cation exchange resin was in the manganese form.
While keeping the towers A to F at 60 ° C., the valves H and P were opened and the valves J, N and O were closed,
300 ml of a liquid containing 10% ribitol was flowed at a rate of 50 ml per minute. Next, the valve H was closed, the valve J was opened, and water was supplied from the pump I at a rate of 50 ml / h. Valve P
When the concentration at the outlet of was 0.2%, the valve P was closed and the valve N was opened. After 20 minutes, valve N was closed and valve O was opened. When the concentration at the outlet of the valve O became 0.2% or less, the valve O was closed and the valve P was opened. This operation was repeated. Table 2 shows the concentrations, weights, and sugar compositions of the effluents obtained in the tanks L and M.
【００４３】[0043]
【表２】 [Table 2]
【００４４】（結晶化）タンクＬの流出液を濃度７７％
まで濃縮し、容量５００ｍｌの結晶化装置へ移し、温度
７０℃から１０時間かけて室温まで冷却した。途中６０
℃にて少量の結晶リビトール粉末をシードとして加え
た。生成した結晶は遠心分離器を用いて分離し、少量の
水で水洗した。得られた結晶は、乾燥後、８２ｇであ
り、液体クロマトグラフィー分析による純度は１００％
であった。(Crystallization) The effluent of the tank L is made to have a concentration of 77%.
The solution was transferred to a crystallizer having a capacity of 500 ml, and cooled from room temperature to 70 ° C. over 10 hours. On the way 60
At 0 ° C., a small amount of crystalline ribitol powder was added as a seed. The generated crystals were separated using a centrifuge and washed with a small amount of water. The obtained crystals weigh 82 g after drying, and the purity by liquid chromatography analysis is 100%.
Met.
【００４５】[0045]
【実施例３】Embodiment 3
【００４６】リビトール製造原料が結晶フラクトースで
ある以外は実施例１と同様の方法で培養を行った。得ら
れた培養液は、その１ｍｌ当たりリビトール４５．０ｍ
ｇ（収率２２．５％）、エリスリトール４５．２ｍｇ
（収率２２．６％）、グリセリン０．８ｍｇ（収率０．
４％）を含有していた。次に遠心分離器を用いてこの培
養液から菌体を分離した後、２０ｇの活性炭（武田薬品
（株）製、白鷺）とともに温度５０℃で１時間撹拌後、
活性炭を濾別し、濾液はカチオン交換樹脂（オルガノ
（株）製、ＩＲ−１２０Ｂ）３００ｍｌ及びアニオン交
換樹脂（オルガノ（株）製、ＩＲＡ−４１０）５００ｍ
ｌを充填したカラムに通液した後、濃度６０％まで濃縮
し、リビトール含有液２２６８ｇを得た。Cultivation was carried out in the same manner as in Example 1 except that the raw material for producing ribitol was crystalline fructose. The obtained culture solution contained 45.0 m of ribitol per ml.
g (yield 22.5%), erythritol 45.2 mg
(Yield 22.6%), glycerin 0.8 mg (yield 0. 2%).
4%). Next, the cells were separated from the culture using a centrifugal separator, and stirred with 50 g of activated carbon (manufactured by Takeda Pharmaceutical Co., Ltd., Shirasagi) at a temperature of 50 ° C. for 1 hour.
The activated carbon was filtered off, and the filtrate was 300 ml of cation exchange resin (IR-120B, manufactured by Organo Co., Ltd.) and 500 m of anion exchange resin (IRA-410, manufactured by Organo Co., Ltd.)
After passing through a column filled with 1, the mixture was concentrated to a concentration of 60% to obtain 2268 g of a ribitol-containing liquid.
【００４７】（クロマト分画）強酸性陽イオン交換樹脂
が鉄形である以外は、実施例１と同様のクロマト分画装
置を使用して、以下のクロマト分画を実施した。Ａ〜Ｆ
の各塔を６０℃に保ちつつ、バルブＨ及びＰを開き、バ
ルブＪ、Ｎ及びＯを閉じた状態で、前記濃度６０％のリ
ビトール含有液３００ｍｌを毎分５０ｍｌの速さで流し
た。次にバルブＨを閉じ、バルブＪを開き、ポンプＩか
ら水を毎時５０ｍｌの速さで供給した。バルブＰの出口
の濃度が０．２％になった時点でバルブＰを閉じ、バル
ブＮを開いた。２２分後、バルブＮを閉じ、バルブＯを
開いた。バルブＯの出口の濃度が０．２％以下になった
時点でバルブＯを閉じ、バルブＰを開いた。この操作を
繰り返した。タンクＬ及びタンクＭに得られた流出液の
濃度、重量及び糖組成は、表３の通りであった。(Chromatographic fractionation) The following chromatographic fractionation was carried out using the same chromatographic fractionation apparatus as in Example 1 except that the strongly acidic cation exchange resin was in the iron form. AF
While maintaining the temperature of each column at 60 ° C., the valves H and P were opened, and the valves J, N and O were closed, and 300 ml of the above-mentioned 60% concentration of ribitol-containing liquid was flowed at a rate of 50 ml per minute. Next, the valve H was closed, the valve J was opened, and water was supplied from the pump I at a rate of 50 ml / h. When the concentration at the outlet of the valve P became 0.2%, the valve P was closed and the valve N was opened. After 22 minutes, valve N was closed and valve O was opened. When the concentration at the outlet of the valve O became 0.2% or less, the valve O was closed and the valve P was opened. This operation was repeated. Table 3 shows the concentrations, weights, and sugar compositions of the effluents obtained in the tanks L and M.
【００４８】[0048]
【表３】 [Table 3]
【００４９】（結晶化）タンクＬの流出液を濃度７７％
まで濃縮し、容量５００ｍｌの結晶化装置へ移し、温度
７０℃から１０時間かけて室温まで冷却した。途中６０
℃にて少量の結晶リビトール粉末をシードとして加え
た。生成した結晶は遠心分離器を用いて分離し、少量の
水で水洗した。得られた結晶は、乾燥後、１０４ｇであ
り、液体クロマトグラフィー分析による純度は９９．９
％であった。(Crystallization) The effluent of the tank L is made to have a concentration of 77%.
The solution was transferred to a crystallizer having a capacity of 500 ml, and cooled from room temperature to 70 ° C. over 10 hours. On the way 60
At 0 ° C., a small amount of crystalline ribitol powder was added as a seed. The generated crystals were separated using a centrifuge and washed with a small amount of water. The obtained crystals weigh 104 g after drying and have a purity of 99.9 according to liquid chromatography analysis.
%Met.
【００５０】[0050]
【実施例４】Embodiment 4
【００５１】リビトール製造原料が砂糖である以外は実
施例１と同様の方法で培養を行った。得られた培養液１
ｍｌ当たりには、リビトール４９．０ｍｇ（収率２４．
５％）、エリスリトール４５．２ｍｇ（収率２２．６
％）、グリセリン１．４ｍｇ（収率０．７％）を含有し
ていた。Cultivation was carried out in the same manner as in Example 1 except that the raw material for producing ribitol was sugar. Obtained culture solution 1
49.0 mg of ribitol per ml (yield 24.
5%), 45.2 mg of erythritol (yield 22.6)
%) And 1.4 mg of glycerin (yield 0.7%).
【００５２】[0052]
【実施例５】Embodiment 5
【００５３】トリコスポロノイデス属に属する微生物と
してトリコスポロノイデス・メガキリエンシス（CBS１
９１．９２）を用い、本培養を１１日間行った以外は、
実施例１と同様の方法で培養を行った。得られた培養液
１ｍｌ当たりには、リビトール２２．６ｍｇ（収率１
１．３％）、エリスリトール４８．６ｍｇ（収率２４．
３％）、グリセリン４．８ｍｇ（収率２．４％）を含有
していた。As a microorganism belonging to the genus Trichosporonoides, Trichosporonoides megachiliensis (CBS1)
91.92), except that the main culture was performed for 11 days.
Culture was performed in the same manner as in Example 1. 22.6 mg of ribitol (yield 1
1.3%) and 48.6 mg of erythritol (yield 24.
3%) and 4.8 mg of glycerin (2.4% yield).
【００５４】[0054]
【００５５】本発明を実施することにより、入手が容易
で且つ安価な原料から、工業的にリビトールを製造する
ことができる。By practicing the present invention, ribitol can be industrially produced from easily available and inexpensive raw materials.
【図１】クロマト分画装置の概略図FIG. 1 is a schematic diagram of a chromatographic fractionation apparatus.
Ａ〜Ｆ 塔 Ｇ 原料仕込みポンプ Ｈ バルブ Ｉ 水仕込みポンプ Ｊ バルブ Ｋ 予熱器 Ｌ 流出液受けタンク Ｍ 流出液受けタンク Ｎ バルブ Ｏ バルブ Ｐ バルブ A to F tower G Raw material charging pump H valve I Water charging pump J valve K Preheater L Outflow liquid receiving tank M Outflow liquid receiving tank N valve O valve P valve
───────────────────────────────────────────────────── フロントページの続き (72)発明者 立野 芳明 静岡県富士市厚原1333−55 (72)発明者 岡本 直記 千葉県松戸市小根本186−２ ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Yoshiaki Tateno 1333-55, Atsuhara, Fuji City, Shizuoka Prefecture (72) Inventor Naoki Okamoto 186-2 Onemoto, Matsudo City, Chiba Prefecture
Claims (5)
を、醗酵性糖質を主炭素源とする培地で培養し、得られ
た培養物からリビトールを採取することを特徴とするリ
ビトールの製造方法。1. A method for producing ribitol, comprising culturing a microorganism belonging to the genus Trichosporonoides in a medium containing fermentable carbohydrate as a main carbon source, and collecting ribitol from the obtained culture.
が、トリコスポロノイデス・オエドセファリスである請
求項１に記載のリビトールの製造方法。2. The method for producing ribitol according to claim 1, wherein the microorganism belonging to the genus Trichosporonoides is Trichosporonoides oeedespharis.
Ｈ３〜５．５、温度２４〜３８℃の範囲内で、好気的条
件で実施される請求項１または２に記載のリビトールの
製造方法。3. The method according to claim 1, wherein the culturing is carried out at a carbohydrate concentration of 10 to 45% by weight,
The method for producing ribitol according to claim 1, wherein the method is performed under aerobic conditions at a temperature of H3 to 5.5 and a temperature of 24 to 38 ° C.
物を陽イオン交換樹脂を充填した塔に供給してリビトー
ル画分を分離するクロマト分画である請求項１〜３の何
れか一つに記載のリビトールの製造方法。4. The method according to claim 1, wherein the collection of ribitol from the culture is a chromatography fraction in which the culture is supplied to a column packed with a cation exchange resin to separate a ribitol fraction. The method for producing ribitol according to the above.
マンガン形または鉄形の何れかの強酸性陽イオン交換樹
脂である請求項４に記載のリビトールの製造方法。5. The cation exchange resin is in the form of aluminum,
The method for producing ribitol according to claim 4, which is a strongly acidic cation exchange resin in either a manganese form or an iron form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9329695A JPH11137285A (en) | 1997-11-14 | 1997-11-14 | Production of ribitol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9329695A JPH11137285A (en) | 1997-11-14 | 1997-11-14 | Production of ribitol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11137285A true JPH11137285A (en) | 1999-05-25 |
Family
ID=18224244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9329695A Pending JPH11137285A (en) | 1997-11-14 | 1997-11-14 | Production of ribitol |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH11137285A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999061648A1 (en) | 1998-05-27 | 1999-12-02 | Mitsubishi Chemical Corporation | Process for producing l-ribose |
| WO2020243190A1 (en) * | 2019-05-28 | 2020-12-03 | The Charlotte Mecklenburg Hospital Authority D/B/A Atrium Health | Compositions and methods of making ribitol |
-
1997
- 1997-11-14 JP JP9329695A patent/JPH11137285A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999061648A1 (en) | 1998-05-27 | 1999-12-02 | Mitsubishi Chemical Corporation | Process for producing l-ribose |
| US6348326B1 (en) | 1998-05-27 | 2002-02-19 | Mitsubishi Chemical Corporation | Process for producing L-ribose |
| WO2020243190A1 (en) * | 2019-05-28 | 2020-12-03 | The Charlotte Mecklenburg Hospital Authority D/B/A Atrium Health | Compositions and methods of making ribitol |
| CN114269712A (en) * | 2019-05-28 | 2022-04-01 | 夏洛特-梅克伦堡医院管理局D/B/A艾奇恩健康 | Compositions and methods for preparing ribitol |
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