JPH10323168A - Anticancer material comprising extract of fuscoporia obliqua cultured in sawdust - Google Patents
Anticancer material comprising extract of fuscoporia obliqua cultured in sawdustInfo
- Publication number
- JPH10323168A JPH10323168A JP10073110A JP7311098A JPH10323168A JP H10323168 A JPH10323168 A JP H10323168A JP 10073110 A JP10073110 A JP 10073110A JP 7311098 A JP7311098 A JP 7311098A JP H10323168 A JPH10323168 A JP H10323168A
- Authority
- JP
- Japan
- Prior art keywords
- birch
- mycelium
- sawdust
- extract
- cultured
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- General Preparation And Processing Of Foods (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
【0001】[0001]
【発明の技術分野】本発明は、培養カバノアナタケの菌
糸体、特にカバノキ類のオガクズ(木片)を主要必須培
地素材とする培地で培養したカバノアナタケ菌糸体の抽
出物を有効成分とする、薬又は健康食品として経口自由
摂取するのに適した抗ガン性物質に関するものである。TECHNICAL FIELD The present invention relates to a medicament or health comprising an extract of birch mycelium cultured on a medium containing, as a main essential medium, a mycelium of cultured birch, especially birch sawdust (wood chips). The present invention relates to an anticancer substance suitable for oral ingestion as a food.
【0002】[0002]
【発明の背景】カバノアナタケ〔学名 Fuscoporia obli
qua(Fr.)Aoshima〕は、天然では山奥の樹木に着生し
て、石炭状の黒色塊に生長しているのが見られるが、本
来は木のガンとして木の生長を阻害しているキノコであ
る。この一般的には樹木の病気として悪者扱いされてき
たキノコを、本発明者は、有効利用する途がないもの
か、食品適性をもたせた企業的利用法はないかものかと
考えて種々研究した結果、カバノアナタケの菌糸体にす
ぐれた抗ガン作用があることを見出し、またこの菌糸体
には飲食物に抗ガン作用を与える食品適性があることを
見出したものである。[Background of the Invention] Birch mushroom [scientific name: Fuscoporia obli
qua (Fr.) Aoshima] is naturally found on trees in the mountains and grows into a coal-like black mass, but originally inhibits tree growth as a tree cancer. It is a mushroom. The present inventor has conducted various studies on this mushroom, which has generally been treated as a bad person as a disease of a tree, as to whether there is no way to use it effectively or whether there is a corporate use method with food suitability. As a result, they have found that the mycelium of B. oleracea has an excellent anticancer effect, and that the mycelium has food suitability to give food and drink an anticancer effect.
【0003】自然界のカバノキ類に付着生育したカバノ
アナタケは、それだけでもガン抑制効果のあることが認
められたが、天然産のカバノアナタケは増殖増大させる
のに年数がかかることや、採集したものを薬や食品に加
工する際の加工適性が悪いこと、また同じものを或る量
だけ集めるのに大変な労働力や長大な時間を要すること
から、天然物をベースとしたのではそもそも企業化には
不向きであった。Birch mushrooms grown and adhered to birch species in nature have been found to have a cancer-suppressing effect by themselves. Due to poor processing suitability when processing into food, and because it takes a lot of labor and long time to collect a certain amount of the same thing, using natural products as a base is not suitable for commercialization in the first place Met.
【0004】例えば、カバノアナタケの粉末をお茶の代
用として用いる場合、天然物は石炭のかけら様の外観で
固く、成分抽出にも時間がかかり、食品に入れるにして
も粉砕するのに時間がかかるなどの難点があり、また風
味もよくないなどが問題であった。[0004] For example, when birch mushroom powder is used as a substitute for tea, the natural product is hard with the appearance of a piece of coal, it takes a long time to extract components, and it takes a long time to pulverize it even in a food. However, there was a problem that the flavor was not good.
【0005】[0005]
【発明の目的】従って本発明は、カバノアナタケに抗ガ
ン作用があることを見出したことに立脚して、それを特
定的な培養方法によって、天然産のカバノアナタケと同
等な諸成分を含有するものとして大量的にかつ一年を通
じて常時培養し、採取し、有効成分を抽出して、経口自
由摂取するのに適した抗ガン性物質、すなわち服用可能
なガン抑制剤及び食品(機能性食品)とすることを目的
とする。Therefore, the present invention is based on the finding that birch mushrooms have an anticancer effect, and it has been determined that they contain various components equivalent to those of naturally occurring birch mushrooms by a specific culture method. Cultured in large quantities and constantly throughout the year, collected, and the active ingredients are extracted to produce anticancer substances suitable for oral ingestion, that is, ingestible cancer suppressants and foods (functional foods). The purpose is to:
【0006】[0006]
【発明の概要】上記の目的を達成するため本発明は、カ
バノアナタケという特殊なキノコについて本発明者が別
に成立させた特定的なオガクズ培養方法を用いて培養さ
れた、天然産のカバノアナタケと同等な諸成分を含有す
るカバノアナタケ菌糸体を、煮出し抽出又は溶剤抽出な
どすることによって得られた液状物、又はこれを乾燥さ
せた粉末を有効成分とし、これに適宜添加物を添加し又
は添加せずに、服用可能な抗ガン剤とすること、及び前
記液状有効成分、又は培養菌糸体を適当な(変色しな
い)温度条件で乾燥して粉末とした上、種々な食品に混
用して抗ガン性機能食品とすること、から成るものであ
る。SUMMARY OF THE INVENTION In order to achieve the above object, the present invention relates to a special mushroom called birch mushroom, which is cultured using a specific sawdust culture method separately established by the present inventors, which is equivalent to a naturally occurring birch mushroom. The birch mycelium containing various components, a liquid obtained by boiled extraction or solvent extraction, or a powder obtained by drying it as an active ingredient, with or without adding additives as appropriate And a liquid antimicrobial agent that can be taken, and the liquid active ingredient or the cultured mycelium is dried under appropriate (non-discoloring) temperature conditions to obtain a powder, which is then mixed with various foods to have an anticancer function. Food.
【0007】本発明の抗ガン性物質(抗ガン剤・抗ガン
性機能食品)を得るのに特定的に必要なカバノアナタケ
菌糸体の培養方法が本発明の基礎をなすので、まずこれ
について説明する。[0007] The method of cultivating the mycelium of B. aeruginosa which is specifically required to obtain the anticancer substance (anticancer agent / anticancer functional food) of the present invention forms the basis of the present invention. .
【0008】培地の調製:カバノアナタケの培養に固有
的に適した培地をつくるため、カバノキ類(シラカバ又
はダケカンバ)の木片すなわちオガクズを容量432cm3の
ミニコンテナに4杯とり、これに消石灰100cc を加え、
さらに米ヌカを前記同様のミニコンテナで1/2 杯加えて
撹拌し、水分が混合物全体の約70%になるように水を加
える。この際、動物体に安全な他の市販の菌糸増殖促進
物を加えてもよい。(例えば、麦のフスマなど) Preparation of culture medium : In order to prepare a culture medium uniquely suited for cultivation of birch mushrooms, four pieces of birch (birch or birch birch) wood chips, or sawdust, are placed in a mini-container having a capacity of 432 cm 3 , and 100 cc of slaked lime are added thereto. ,
Further, half a cup of rice bran is added in the same mini-container and stirred, and water is added so that the water content is about 70% of the whole mixture. At this time, another commercially available hyphal growth promoter safe to the animal body may be added. (For example, wheat bran)
【0009】上記の混合物を耐熱性の袋又は容器に入
れ、無菌状態になるように加熱する。通常100℃で8時
間水蒸気の蒸し釜(レトルト容器でもよい)に入れる。
この蒸熱は滅菌のためであり、シラカバ又はダケカンバ
に本来含有されている種々な木質成分、特にフエノール
性水酸基やアルコキシ基をもつ化合物や多糖体を溶出除
去させることはない。以上の要領で滅菌したものをオガ
クズ培地とし、これを植菌に先立ち冷却する。冷却時、
外気を袋又は容器に吸引するので、雑菌が入らないよう
に注意する。The above mixture is placed in a heat-resistant bag or container and heated so as to be sterile. Usually put in a steam steamer (retort container may be used) at 100 ° C for 8 hours.
This steaming is for sterilization and does not elute and remove various woody components originally contained in birch or birch birch, particularly compounds or polysaccharides having a phenolic hydroxyl group or an alkoxy group. The sterilized medium as described above is used as a sawdust medium, which is cooled prior to inoculation. During cooling,
Care should be taken not to enter any germs as the outside air is sucked into the bag or container.
【0010】植菌:オガクズ培地の中心温度がカバノア
ナタケ菌糸の生育温度、好適に20℃以下に下がった頃、
カバノアナタケの純粋菌糸を植菌する。周囲が無菌の環
境を保てるような部屋を作り、培地を入れてある袋又は
容器の蓋をする。植菌用のスプーン等の器具は、雑菌を
防止するため、焼いて滅菌する。作業者の手はアルコー
ル等で消毒し、マスクをし、白衣等を着て雑菌の落下混
入をさせないようにする。植菌する時、培地の床の真中
に直径2〜3cm程度の穴を下向きに(袋の場合は、袋を
破らないように)あけるとよい。 Inoculation : When the center temperature of the sawdust medium drops to the growth temperature of the birch mycelium, preferably 20 ° C. or less,
Inoculate a pure hypha of birch. Create a room where the surroundings can maintain a sterile environment, and cover the bag or container containing the medium. Instruments such as spoons for inoculation are baked and sterilized to prevent germs. Worker's hands should be disinfected with alcohol or the like, put on a mask, put on a white coat, etc., so as to prevent falling and mixing of various bacteria. At the time of inoculation, a hole having a diameter of about 2 to 3 cm may be opened downward (in the case of a bag, so as not to break the bag) in the center of the medium floor.
【0011】高さ約15cm、底辺の一辺が約10cm角の培地
であれば、真中に1ケ所直径2〜3cm程度の穴を約10cm
位の深さにあけておくと菌糸をより早くオガクズ培地に
増殖させることができるので、好適である。植菌時に
は、その穴をめがけて菌糸をばらまくと、培地の上面に
も菌糸が散らばるので好都合である。培地が大きいもの
の場合も(深さが15cmとして)、10cm角に1ケ所の割合
で、直径2〜3cm、深さ約10cmの穴をあけるとよい。If the medium is about 15 cm high and one side of the medium is about 10 cm square, a hole having a diameter of about 2 to 3 cm is formed in the center at about 10 cm.
It is preferred that the mycelium be grown at a depth of about 1 to allow the mycelium to grow on the sawdust medium more quickly. At the time of inoculation, it is convenient to disperse the mycelium at the hole so that the mycelium is scattered on the upper surface of the medium. Even in the case of a large medium (assuming a depth of 15 cm), a hole having a diameter of 2 to 3 cm and a depth of about 10 cm is preferably formed in one 10 cm square.
【0012】比較例として、シラカバ以外の樹木のオガ
クズを使った実験をした。すなわち落葉樹(例えばブ
ナ、ナラ)、トド松などのオガクズで作った培地を使っ
て、シラカバ培地で行ったと同様な方法を試験したが、
これらカバノキ類以外のオガクズの培地ではカバノアナ
タケの菌糸は生育できなかった。ここから、カバノアナ
タケの菌糸を人工的に生育させるには、特定的にカバノ
キ類の樹木、特にシラカバやダケカンバのオガクズ(木
片)を培地の主要必須素材として用いることが必要条件
であることが認められる。As a comparative example, an experiment was conducted using sawdust of trees other than birch. In other words, using a medium made of sawdust such as deciduous trees (eg, beech and oak) and fir pine, the same method as that performed on the birch medium was tested.
In the medium of sawdust other than these birch species, the hypha of birch was not able to grow. From this, it is recognized that it is necessary to specifically use birch trees, especially birch and birch sawdust (wood chips) as the main essential material of the culture medium in order to artificially grow the mycelium of the birch. .
【0013】生育:上記のように植菌した培地は、13℃
から33℃の温度条件におくのがよい。あまり寒くても暑
くも菌糸は良好な発育をしない。企業規模の培養の目安
としては、20℃程度を目安として温度管理するとよく、
室内温度を20℃付近とすれば、雑菌やカビの飛散が少な
く、培養中に培地がカビに犯されるなどの危険が少なく
なるので好ましい。 Growth : The medium inoculated as described above is at 13 ° C.
It is good to keep the temperature condition from to 33 ° C. Mycelium does not grow well, even if it is too cold or hot. As a guideline for cultivation on a company scale, it is better to control the temperature with about 20 ° C as a guide,
An indoor temperature of about 20 ° C. is preferable because germs and mold are less scattered, and the danger that the culture medium is violated by mold during culture is reduced.
【0014】容器に入れた培養物は、空気の流通のよい
棚に上げ、1日1〜2回潅水し、室内湿度は80%以上を
維持するように努める。1日2回くらい床面も乾燥しな
いように散水するとよい。オガクズ培地の大きさにもよ
るが、通常4ケ月程度で菌糸が培地に真白く生育し、培
地表面全体に充満する。The culture placed in the container is placed on a shelf with good air circulation, and is irrigated once or twice a day. The room humidity is maintained so as to maintain 80% or more. It is advisable to spray water twice a day so that the floor does not dry out. Although it depends on the size of the sawdust medium, the hypha usually grows white in the medium in about four months and fills the entire surface of the medium.
【0015】採取:生育した菌糸体は、適宜採取し、特
定目的に応じた加工(例えば、有効成分の抽出、抽出液
の濃縮或いは希釈、乾燥粉末化、焼成、等々)をした
上、薬として又は機能性食品として使用することができ
る。シラカバオガクズ培地で培養した菌糸体は、液体培
地で培養した菌糸体に比べ、凍結乾燥などにより粉末化
する工程が容易であり、またその粉末が長く乾燥状態を
保つことができ、保存性がよい利点がある。カバノキ類
のオガクズ培地を使用することにより、得られるカバノ
アナタケ菌糸体は、天然産に匹敵する強力な有効成分を
含有していることが、後記のマウス実験からもうかがえ
る。 Collection : The grown mycelium is appropriately collected, processed according to a specific purpose (for example, extraction of an active ingredient, concentration or dilution of an extract, dry powdering, baking, etc.) and then as a drug. Or it can be used as a functional food. Mycelium cultured in white birch waste medium is easier to pulverize by freeze-drying and the like than mycelium cultured in a liquid medium, and the powder can be kept in a dry state for a long time and has good storage stability. There are advantages. The use of the birch sawdust medium as described above suggests that the resulting birch mycelium contains a potent active ingredient comparable to that of natural products, as described in the mouse experiments described below.
【0016】以上の特定的なカバノアナタケのオガクズ
培養方法により得られたカバノアナタケ菌糸体の抗ガン
性、特に経口自由摂取によりガン抑制作用があることに
ついて、マウス実験により効果を確認した。The effect of the birch mycelium obtained by the above-described method for cultivating birch mushrooms on the specific birch mushrooms, in particular, on the anticancer properties, in particular, the ability to suppress cancer by oral ingestion, was confirmed by mouse experiments.
【0017】試験目的:カバノアナタケFuscoporia obl
iqua (Fr.) Aoshima からの抽出液の in vivo(生体内)
抗腫瘍作用をマウス実験腫瘍を使った系にて評価する。 Test purpose : Fucoporia obl
In vivo of extract from iqua (Fr.) Aoshima
The antitumor effect is evaluated in a system using a mouse experimental tumor.
【0018】試験方法: 1.実験動物 BALB/cマウス(雄) 5〜6週齢 2.実験腫瘍 Meth A fibrosarcoma(メチルコラントレン誘発腺維肉
腫) 3.カバノアナタケ抽出液の投与法 3-1 試験液の調整法:検体(培地から採取した菌糸体
及び菌糸の生着あるオガクズ)300 gを蒸留水3L(リッ
トル)で100℃、20分間煮出し抽出した。抽出液を濾過し
たのち、使用まで−20℃で保存した。なお、菌糸の生着
がないオガクズから同様に抽出した液を対照として用い
た。 3-2 投与経路:経口自由摂取 4.治療実験 BALB/cマウスに腫瘍細胞(Meth Aの場合 2×1
06個)を右腹側部に皮下移植し、Meth A 移植後9日目
よりマウスにカバノアナタケ抽出液(治療群)あるいは対
照抽出液(対照群)を経口自由摂取させた。週2回、各マ
ウスの腫瘍容積を測定し、対照群と治療群を比較した。 5.腫瘍容積の測定 腫瘍塊の長径、短径をキャリパーで測定し、以下の式で
算出した。 腫瘍容積(mm2)=1/2×(長径)×(短径)2 6.統計的有意差検定 Mann-Whitney U-test (P<0.05)[0018]Test method1. Experimental animal BALB / c mouse (male) 5-6 weeks old 2. Experimental tumor Meth A fibrosarcoma (methylcholanthrene-induced glandular fibrosis
Tumor) 3. Administration method of birch mushroom extract 3-1 Preparation of test solution: specimen (mycelium collected from culture medium)
3 g of distilled water (300 g)
At 100 ° C for 20 minutes. Filter the extract
After that, it was stored at -20 ° C until use. In addition, engraftment of hypha
A similar extract from sawdust without grease was used as a control.
Was. 3-2 Administration route: oral ingestion 4. Therapeutic experiment Tumor cells (in the case of Meth A 2 × 1) in BALB / c mice
06) Was implanted subcutaneously in the right flank, 9 days after Meth A transplantation
More mice were given birch extract (treatment group) or
The light extract (control group) was orally freely ingested. Twice a week, each
The tumor volume of the mouse was measured, and the control group and the treatment group were compared. 5.Measurement of tumor volume Measure the major axis and minor axis of the tumor mass with calipers and use the following formula
Calculated. Tumor volume (mmTwo) = 1/2 × (major axis) × (minor axis)Two 6. Statistical significance test Mann-Whitney U-test (P <0.05)
【0019】比較結果: Meth Aに対する治療実験 図1に対照群及び治療群の腫瘍増殖曲線を示した。治療
群の腫瘍容積は、腫瘍移植後16日目より統計的有意差
(P<0.05)をもって抑制された。 注:抽出液のかわりに水道水を自由摂取させた群の腫瘍
容積推移は、本実験の対照群と同様であった。 Comparative Results : Treatment Experiment for Meth A FIG. 1 shows the tumor growth curves of the control group and the treatment group. Tumor volume of treatment group was statistically significant from day 16 after tumor implantation
(P <0.05). Note: The change in tumor volume in the group that had free access to tap water instead of the extract was similar to the control group in this experiment.
【0020】結論:特定培地で培養した本発明に係るカ
バノアナタケ抽出液の抗腫瘍効果を担癌マウスに経口自
由摂取させることにより評価した結果、本発明の特定培
養カバノアナタケ抽出液はMeth A(メチルコラントレン
誘発腺維肉腫)に対して明らかに有効であった。 Conclusion : The anti-tumor effect of the birch mushroom extract of the present invention cultured in a specific medium was evaluated by ingesting a tumor-bearing mouse freely orally. Tren-induced fibrosarcoma).
【0021】こうして、前記した特定的な培養方法によ
り培養したカバノアナタケ菌糸体の抽出物は、経口自由
摂取の方法によって、図1に示すように、明らかな抗ガ
ン作用のあることが認められる。この特定的なカバノキ
類オガクズ培地で得られたカバノアナタケ菌糸体は、マ
ウス実験が、菌糸体抽出物の強制投与ではなく、経口自
由摂取で行なわれた点できわめて重要かつ有意義であ
り、このカバノアナタケが薬や食品などとして口から自
然に摂取するのに適した性状(味、香りなど)をもってい
ること、そして薬効成分が生体内で強力に作用すること
を示している。(口から自由意志で摂取した薬物は、胃
酸や体内の生理反応などにより薬効が減殺されるのが通
常である。強い薬効成分をもっていないと、口から飲ん
でも効かないことが多い。)口から自然に摂取できると
いう性状は、本発明で用いる培養菌糸体が、抗ガン性の
食品や薬などとして経口自由摂取できる(服用可能な)
物質として加工できる広い応用範囲をもつことを示して
いる。Thus, it is recognized that the extract of B. amanita mycelium cultured by the above-mentioned specific culture method has a clear anticancer effect as shown in FIG. 1 by the method of oral free intake. The birch mycelium obtained in this particular birch sawdust medium is extremely important and significant in that mouse experiments were performed by oral, not gavage, administration of the mycelium extract. It has properties (taste, aroma, etc.) suitable for natural ingestion from the mouth as medicines and foods, and shows that medicinal ingredients act strongly in vivo. (Drugs taken freely by mouth are usually diminished in efficacy due to stomach acid and physiological reactions in the body. If you do not have a strong medicinal component, it will often be ineffective even if you take it by mouth.) The nature that it can be taken naturally means that the cultured mycelium used in the present invention can be freely taken orally as an anticancer food or drug (can be taken).
It has a wide range of applications that can be processed as a substance.
【0022】本発明は、上記のように特定的な培養方法
により得られたカバノアナタケ菌糸体を採取し、これを
煮出し抽出によって、或いは溶剤抽出によって液状の有
効成分を得る。煮出し抽出の実際例は、前記した試験方
法の3.で述べたのと基本的に同じであるが、使用する水
の種類(蒸留水か否か)、水と菌糸体の割合、抽出温
度、及び抽出時間は、特に限定されるものではない。抽
出液の保存温度も同様に限定的ではない。カバノアナタ
ケ抽出物は、液状のまま、希釈し、又は希釈しないで、
経口服用することができる。多量の水で煮出し抽出した
場合は、逆に、抽出液を濃縮することができる。これら
の、希釈した、又は希釈しない、或いは濃縮した液状物
が本発明の抗ガン性物質である。また、抽出液を凍結乾
燥又は加熱乾燥などにより粉末化し、その粉末を、水と
ともに、又は水に溶かして服用することもできる。この
場合、粉末そのもの、或いはその水溶液が本発明の抗ガ
ン性物質である。According to the present invention, the mycelium of B. oleracea obtained by the specific culturing method as described above is collected, and the active ingredient in liquid form is obtained by extraction by boiling or extraction with a solvent. The actual example of boiled-out extraction is the test method described above.
Basically the same as described in section 3 of the method , except that the type of water used (whether or not distilled water), the ratio of water to mycelium, the extraction temperature, and the extraction time are particularly limited. is not. The storage temperature of the extract is likewise not limiting. Birch mushroom extract, as it is, diluted or undiluted,
Can be taken orally. On the contrary, when the extract is boiled and extracted with a large amount of water, the extract can be concentrated. These diluted, undiluted or concentrated liquids are the anticancer substances of the present invention. Alternatively, the extract may be powdered by freeze drying or heat drying, and the powder may be taken together with or dissolved in water. In this case, the powder itself or its aqueous solution is the anticancer substance of the present invention.
【0023】本発明の抗ガン剤としての使用の一例を述
べれば、人間では、例えば胃ガンであれば、体重60Kg
の人を標準として月に6kg(20g/日)前後を目安として同
量の水で煎じて飲むと制ガン作用のあることが認められ
る。水溶液として自然に飲めることは服用品として非常
に有意義なことである。本発明で用いるカバノアナタケ
培養菌糸体は、従来知られている液体培養物に比べて、
例えば凍結乾燥法による粉末化が容易であるとともに、
粉末が長期にわたって乾燥状態を保持できるので、商品
化に優れている。As an example of the use as an anticancer agent of the present invention, for humans, for example, for stomach cancer, the body weight is 60 kg.
It is recognized that there is an anti-cancer effect when decoction is taken with the same amount of water, with about 6 kg (20 g / day) per month as a guideline for a standard person. Drinking naturally as an aqueous solution is very significant for clothing. The cultured mycelium of the birch mushroom used in the present invention, compared to a conventionally known liquid culture,
For example, powderization by freeze-drying method is easy,
Since the powder can be kept dry for a long period of time, it is excellent in commercialization.
【0024】本発明の抗ガン性物質の食品としての応用
は色々あるが、食品混入物とするため、まず、カバノア
ナタケのオガクズ培養物を、変色しない条件下で加熱乾
燥、又は真空低温乾燥する。この時カバノアナタケの培
養物を適宜100 ℃で加熱滅菌した上で乾燥を行ってもよ
い。加熱乾燥は、変色しない温度で行わなければ、風味
が良くなく食品適性を損なう。高温で乾燥すると焦げ臭
くなり、有効成分も減少するので注意が必要である。There are various applications of the anticancer substance of the present invention as a food, but in order to make it a food contaminant, first, a sawdust culture of birch is dried by heating or drying under vacuum at a low temperature. At this time, the culture of the birch mushroom may be appropriately heat-sterilized at 100 ° C. and then dried. If the heat drying is not performed at a temperature that does not cause discoloration, the flavor is not good and food suitability is impaired. Care must be taken as drying at high temperatures will result in a burning odor and reduced active ingredients.
【0025】以上のような培養カバノアナタケ菌糸乾燥
物を、例えばティーバッグ詰めにすると、企業化容易な
キノコ茶ができる。このキノコ茶は、日常的に服用でき
るので健康生活にも至便である。乾燥した培養物は粒子
状になっているので、種々の後加工がしやすく、必要に
応じてさらに微粉末化し、またはエキスを抽出すること
により、多様な食品又は飲物(本明細書では飲物を含め
て「食品」と総称する)に混合して服用可能とすることが
できる。When the dried mycelium of the above-described cultured birch is obtained, for example, packed in a tea bag, mushroom tea that can be easily commercialized is obtained. Since this mushroom tea can be taken on a daily basis, it is also convenient for healthy living. Since the dried culture is in the form of particles, it is easy to carry out various post-processing, and if necessary, further pulverized or extracted to obtain various foods or drinks (in this specification, drinks and drinks). (Collectively referred to as "foods").
【0026】本発明の培養カバノアナタケ抽出物を混合
することが可能な食品の一例を示すと、ガム、キャラメ
ル、キャンデー、チョコレート、ジャム、麺、味噌、ド
リンク剤、ペットフード、肉だんご、病人の流動食、調
味料(味噌・醤油・スープの素等)、パン、ドーナツ、
卵焼き、ソーセージ、ハム、魚肉練りもの、海苔の佃
煮、だんご、きびだんご、ワイン、ビール、焼酎、ウイ
スキー、清涼飲料、果実又は野菜ジュース、緑茶、番
茶、抹茶、ウーロン茶等々である。以上のような食品
に、本発明の培養カバノアナタケ乾燥物の粉末又は抽出
エキスを混ぜることにより、異味異臭などを生じること
はなく、経口摂取が容易で、しかも制ガン作用のある便
利な機能性食品とすることができる。Examples of foods which can be mixed with the cultured birch extract of the present invention include gum, caramel, candy, chocolate, jam, noodles, miso, drinks, pet food, meat dumplings, and the flow of sick people. Food, seasonings (miso, soy sauce, soup ingredients, etc.), bread, donut,
Egg fried, sausage, ham, fish paste, seaweed tsukudani, dango, kibi-dango, wine, beer, shochu, whiskey, soft drink, fruit or vegetable juice, green tea, bancha, matcha, oolong tea, and the like. By mixing the above-mentioned foods with the powder or extract of the cultured dried birch mushroom of the present invention, it does not cause off-flavors or the like, is easy to ingest, and is a convenient functional food having an anticancer effect. It can be.
【0027】[0027]
【発明の効果】以上述べたように、本発明は従来見向き
もされなかった樹木の病原菌を動物の抗ガン性物質とし
て活用する道を開いたもので、特に天然物では難しい有
効菌糸体のスピーディーな人工的増殖法を基礎として、
従来の液体培養では得られなかった、天然産と同等に強
力な有効成分を含有保持している菌糸体を大量生産的に
採取し、加工し、経口自由摂取に適した抗ガン性物質
(薬・食品)とした点で、人類及びその他の動物の健康
の保持・増進に益する著大な効果を上げることができ
る。As described above, the present invention paves the way for utilizing pathogens of trees, which have not been considered in the past, as anti-cancer substances for animals. Based on a simple artificial propagation method,
Anticancer substances (drugs) suitable for oral ingestion that are not obtained by conventional liquid culture, and are collected and processed in mass production of mycelium containing active ingredients that are as powerful as those of natural products. Food), it can have a significant effect on the maintenance and promotion of human and other animal health.
【図1】は、本発明に係るカバノアナタケ培養物を経口
自由摂取させたマウスによる腫瘍治療実験結果(黒丸)
を、対照例(白丸)と比較して示すグラフで、縦軸は腫瘍
容積(mm2)を、横軸は腫瘍移植後の日数を表わす。BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows the results of a tumor treatment experiment using a mouse to which a birch mushroom culture of the present invention was orally freely ingested (black circles).
Is a graph showing a comparison with the control example (open circles), in which the vertical axis represents tumor volume (mm 2 ) and the horizontal axis represents days after tumor implantation.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 FI C12R 1:645) ──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 6 Identification code FI C12R 1: 645)
Claims (1)
選ばれたカバノキ類の樹木のオガクズを主要必須培地素
材とし、その本来含有している成分を損なわないように
調製した培地で培養生育させた、天然産と同等な諸成分
を含有するカバノアナタケ菌糸体を、煮出し抽出又は溶
剤抽出して得られた液状物又は乾燥粉末を有効成分とす
る、薬又は食品として経口自由摂取するのに適した抗ガ
ン性物質。Claims: 1. A natural birch tree birch tree selected from the group consisting of birch and birch birch is used as a main essential medium material and grown and grown on a medium prepared so as not to impair the components originally contained therein. Anticancer properties suitable for oral or oral ingestion as a drug or food, with liquid or dry powder obtained by boiling or solvent-extracting the mycelium of birch mushrooms containing various components equivalent to those produced in Japan. material.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10073110A JPH10323168A (en) | 1998-03-07 | 1998-03-07 | Anticancer material comprising extract of fuscoporia obliqua cultured in sawdust |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10073110A JPH10323168A (en) | 1998-03-07 | 1998-03-07 | Anticancer material comprising extract of fuscoporia obliqua cultured in sawdust |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1114665A Division JP3008292B2 (en) | 1989-05-07 | 1989-05-07 | Method for producing birch mycelium |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH10323168A true JPH10323168A (en) | 1998-12-08 |
Family
ID=13508828
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10073110A Pending JPH10323168A (en) | 1998-03-07 | 1998-03-07 | Anticancer material comprising extract of fuscoporia obliqua cultured in sawdust |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH10323168A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004315512A (en) * | 2003-03-28 | 2004-11-11 | Unitika Ltd | Functional composition |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5026448B1 (en) * | 1970-01-09 | 1975-09-01 | ||
JPS5244613B1 (en) * | 1976-12-27 | 1977-11-09 | ||
JPS5394023A (en) * | 1977-01-29 | 1978-08-17 | Kureha Chem Ind Co Ltd | Preparation of anti-tumor polysaccharide |
JPS5611724A (en) * | 1979-07-07 | 1981-02-05 | Kunio Narita | *matsutake* *mushroom* cultivation method |
JPS5623814A (en) * | 1979-08-01 | 1981-03-06 | Hideo Fujimoto | Cultivation of mushroom *maetake* |
-
1998
- 1998-03-07 JP JP10073110A patent/JPH10323168A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5026448B1 (en) * | 1970-01-09 | 1975-09-01 | ||
JPS5244613B1 (en) * | 1976-12-27 | 1977-11-09 | ||
JPS5394023A (en) * | 1977-01-29 | 1978-08-17 | Kureha Chem Ind Co Ltd | Preparation of anti-tumor polysaccharide |
JPS5611724A (en) * | 1979-07-07 | 1981-02-05 | Kunio Narita | *matsutake* *mushroom* cultivation method |
JPS5623814A (en) * | 1979-08-01 | 1981-03-06 | Hideo Fujimoto | Cultivation of mushroom *maetake* |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004315512A (en) * | 2003-03-28 | 2004-11-11 | Unitika Ltd | Functional composition |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20080286388A1 (en) | Pharmaceutical Composition and Non Dependence Coffee Comprising Edible Carboxylic Acid and/or Its Acid Salts and Coffeine | |
KR101432584B1 (en) | Method for steamed and fermented Codonopsis lanceolata for boosting biological activity | |
KR100552398B1 (en) | An alcohol detoxification beverage comprising extract of horse bean | |
JP3008292B2 (en) | Method for producing birch mycelium | |
KR20170065452A (en) | Lotus tea beverage and manufacturing method thereof | |
KR20150083638A (en) | Composition for enhancing growth and reinforcing immunity | |
JP3178822B2 (en) | 3α-hydroxysteroid dehydrogenase inhibitory activator and hyaluronidase inhibitory activator | |
JPH10323168A (en) | Anticancer material comprising extract of fuscoporia obliqua cultured in sawdust | |
CN107897719B (en) | Natto composite preparation and preparation method thereof | |
KR100707342B1 (en) | Functional food using mulberry, leek and white clay and a method for producing thereof | |
KR20160082568A (en) | Composition comprising Rhus verniciflua for extraction and extraction mthod of the Composition | |
Eliseeva et al. | Wormwood (lat. Artemísia) | |
CN110432476A (en) | A kind of method for salting of Antimicrobial preservative leek flower sauce | |
JP2020063216A (en) | Oral composition | |
JP5986285B1 (en) | Calcium absorption promoter | |
KR101752547B1 (en) | A fermentation method of some races of gingers by using vitamin C | |
CN108850380A (en) | A kind of formula and preparation method of Chinese wolfberry and coffee | |
KR102621081B1 (en) | Method for manufacturing seasoning sauce for shellfish and seafood | |
JPH10120589A (en) | Antibacterial agent and antibacterial food | |
KR102000165B1 (en) | Method for manufacturing the health functional food containing butterbur, and the health functional food manufactured by the method | |
KR102636322B1 (en) | Method for manufacturing dried yellow corvina with gochujang and dried yellow corvina with gochujang produced thereby | |
CN107753752A (en) | A kind of relieving alcoholism and protecting liver stem of noble dendrobium electuary | |
JP6716623B2 (en) | Composition showing erythropoietin-inducing action and method for producing the same | |
KR101955883B1 (en) | Producing method of Functional Compositions Using Plant Extracts Showing Biological Activity and the Composition | |
KR101905329B1 (en) | Moringa red pepper paste including Chaga mushroom and manufacturing method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20040113 |