JPH10319023A - Vessel holding device - Google Patents
Vessel holding deviceInfo
- Publication number
- JPH10319023A JPH10319023A JP9125368A JP12536897A JPH10319023A JP H10319023 A JPH10319023 A JP H10319023A JP 9125368 A JP9125368 A JP 9125368A JP 12536897 A JP12536897 A JP 12536897A JP H10319023 A JPH10319023 A JP H10319023A
- Authority
- JP
- Japan
- Prior art keywords
- holding
- container
- reaction
- sample
- holes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、生化学、免疫化学
の分野において使用される分析装置用の容器保持装置に
関するものであり、特に好適には、血液、血清、尿等、
その保存のために適当な温度とその分析のために適当な
温度が異なる試料を取り扱う、簡便な構成の小型免疫化
学分析装置用容器保持装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a container holding device for an analyzer used in the fields of biochemistry and immunochemistry, and particularly preferably blood, serum, urine, etc.
The present invention relates to a container holding device for a small-sized immunochemical analyzer having a simple structure that handles samples having different temperatures suitable for storage and suitable temperatures for analysis.
【０００２】[0002]
【従来の技術】免疫化学分析装置等においては、試料容
器に収容された状態で供給される血清等の生体試料を、
まず分注装置による分注位置に搬送し、該装置を用いて
吸引し、別途搬送される反応に必要な標識抗体等の試薬
が予め投入され又は後に投入される反応容器に吐出し、
一定時間、免疫反応を生じさせるのに適当な温度条件下
におき、そして例えば蛍光測定等を行う。2. Description of the Related Art In an immunochemical analyzer or the like, a biological sample such as serum supplied in a state accommodated in a sample container is
First, transported to a dispensing position by a dispensing device, aspirated using the device, a reagent such as a labeled antibody required for a separately transported reaction is previously charged or discharged into a reaction container to be charged later,
For a certain period of time, it is left under a temperature condition suitable for generating an immune reaction, and for example, a fluorescence measurement is performed.
【０００３】従来、かかる分析装置においては、試料容
器はターンテーブル、スネークチェーン、ラック等に保
持された状態で分注装置による分注位置（吸引位置）に
搬送されるのが普通である。一方、反応容器は、一定温
度に温調されたターンテーブル、スネークチェーン、ラ
ック等に保持された状態で分注装置による分注位置（吐
出位置）に搬送するか、或いは、試料を分注した後に温
調されたターンテーブルやスネークチェーン等に搬送す
るのが普通である。そして分析の際、反応に必要な試薬
を別途反応容器に分注する場合には、試薬容器を別途ラ
ック等に保持しておき、分注装置を用いてターンテーブ
ル等に保持された反応容器に試薬を分注するのが普通で
ある。Conventionally, in such an analyzer, the sample container is usually conveyed to a dispensing position (suction position) by a dispensing device while being held on a turntable, a snake chain, a rack or the like. On the other hand, the reaction container was transported to a dispensing position (discharge position) by a dispensing device while being held on a turntable, a snake chain, a rack, or the like, which was adjusted to a constant temperature, or a sample was dispensed. Usually, it is usually transported to a temperature-controlled turntable or snake chain. When separately dispensing the reagents required for the reaction into the reaction vessel during analysis, hold the reagent vessel separately in a rack or the like, and use the dispensing device to place the reagent vessel in the reaction vessel held on a turntable or the like. It is common to dispense reagents.
【０００４】[0004]
【発明が解決しようとする課題】前述した従来の分析装
置では、試料容器、反応容器そして試薬容器を、それぞ
れ独立したターンテーブル、スネークチェーン、ラック
等に保持するため、１台の装置中に複数の保持装置が含
まれ、その結果、全体として装置が大型化するという課
題がある。また、分注装置をこれら保持装置間を移動さ
せる必要があるため、その移動手段が複雑化するという
課題もある。In the above-mentioned conventional analyzer, a plurality of sample vessels, reaction vessels and reagent vessels are held in independent turntables, snake chains, racks and the like, so that a plurality of vessels are provided in one apparatus. There is a problem that the size of the device as a whole increases as a result. In addition, since the dispensing device needs to be moved between these holding devices, there is a problem that the moving means is complicated.
【０００５】また、例えば免疫化学分析装置では、試料
については特別の温調を行わないものの、反応容器は通
常温調し、試薬についてはその種類によって温調を行う
ことが行われる。従って、温調手段についても、反応容
器を担持するための保持部と試薬容器を保持するための
保持部の２箇所に設置することが必要になる。しかも、
前述のように試薬容器を担持するための保持部について
は、温調を行わない種類の試薬が混在する際は温調手段
を有する部分と、温調手段を有さない部分を別途設ける
必要がある。[0005] In an immunochemical analyzer, for example, a special temperature control is not performed on a sample, but a reaction vessel is usually controlled on a temperature basis, and a reagent is controlled on a temperature-dependent basis. Therefore, it is necessary to install temperature control means at two places, that is, a holding part for holding the reaction container and a holding part for holding the reagent container. Moreover,
As described above, it is necessary to separately provide a portion having a temperature control unit and a portion not having a temperature control unit for a holding portion for supporting a reagent container when a type of reagent not performing temperature control is mixed. is there.
【０００６】従って本発明の目的は、非常に簡便な構成
で、分注装置の移動を最小限にし得、しかも単一の温調
手段で分析の実行に支障のない温調を実現し得る容器保
持装置を提供することにある。Accordingly, an object of the present invention is to provide a container which can minimize the movement of the dispensing apparatus with a very simple structure and can realize a temperature control which does not hinder the execution of analysis by a single temperature control means. It is to provide a holding device.
【０００７】[0007]
【課題を解決するための手段】上記目的に鑑みて成され
た本発明の容器保持装置は、試料を収容する試料容器を
保持するための１列の保持穴を有する試料容器保持部
と、反応用の反応容器を保持するための１列の保持穴を
有する反応容器保持部と、これら保持部の保持穴が平行
となるように両保持部を連結する連結手段と、連結され
た両保持部を保持穴の列方向に水平移動するための水平
移動手段とからなるものである。反応容器については温
調が必要であり、一方、試料容器については温調が不要
である場合には、反応を生じさせるのに適当な温度範囲
に温調するための温調手段を反応容器保持部に取り付け
ると共に、試料容器保持部を該温調手段の温調作用から
熱的に遮断すれば良い。SUMMARY OF THE INVENTION In view of the above, a container holding apparatus according to the present invention has a sample container holding portion having a row of holding holes for holding a sample container for holding a sample, and a reaction container. Container holding portion having a row of holding holes for holding reaction vessels for use, connecting means for connecting both holding portions such that the holding holes of these holding portions are parallel, and both connected holding portions And horizontal moving means for horizontally moving the holding holes in the row direction of the holding holes. If temperature control is required for the reaction vessel, but temperature control is not required for the sample vessel, the temperature control means for controlling the temperature to an appropriate temperature range for causing the reaction is held in the reaction vessel. At the same time, the sample container holding section may be thermally isolated from the temperature control action of the temperature control means.
【０００８】かかる構成を有する本発明の容器保持装置
においては、試料容器保持部に設けられた保持穴間の離
間距離と反応容器保持部に設けられた保持穴間の離間距
離を共に一定かつ同一とし、水平移動手段が１回の作動
により該離間距離分、両保持部を水平移動し得るように
することが好ましい。かかる構成によれば、単一の移の
制御等、移動操作を極めて簡便化することが可能だから
である。ここで、更に試料容器保持部に設けられた保持
穴と反応容器保持部に設けられた保持穴が、これら保持
部の水平移動方向に垂直な直線上に位置するように連結
手段により両保持部を連結しておけば、分注装置を該直
線上を往復動するように構成する一方、前記水平移動手
段による水平移動についても、制御が容易になる。In the container holding apparatus of the present invention having such a configuration, the distance between the holding holes provided in the sample container holding portion and the distance between the holding holes provided in the reaction container holding portion are both constant and the same. It is preferable that the horizontal moving means can horizontally move both holding portions by the distance by one operation. According to such a configuration, it is possible to extremely simplify a moving operation such as a single moving control. Here, both holding portions are connected by the connecting means such that the holding hole provided in the sample container holding portion and the holding hole provided in the reaction container holding portion are positioned on a straight line perpendicular to the horizontal movement direction of these holding portions. Is connected, the dispensing apparatus is configured to reciprocate on the straight line, while the horizontal movement by the horizontal moving means is easily controlled.
【０００９】本発明の容器保持装置においては、試料容
器保持部及び／又は反応容器保持部に設けられた保持穴
の一部を、反応に必要な試薬を収容する試薬容器を保持
するための保持穴として使用することができる。これに
より、試薬容器保持用の保持部を別個に配置する必要が
ない。また、前記のように温調手段を反応容器保持部に
取り付けると共に試料容器保持部を該温調手段の温調作
用から熱的に遮断した構成においては、温調が必要な試
薬容器については反応容器保持部の保持穴に、温調が不
要な試薬容器については試料容器保持部の保持穴に置く
ようにすれば、複数の温調手段を配置する必要がなくな
る。In the container holding device of the present invention, a part of the holding hole provided in the sample container holding part and / or the reaction container holding part is used to hold a reagent container for holding a reagent necessary for the reaction. Can be used as a hole. Thus, there is no need to separately arrange a holding section for holding the reagent container. Further, in the configuration in which the temperature control means is attached to the reaction container holding portion and the sample container holding portion is thermally cut off from the temperature control action of the temperature control means as described above, the reaction of the reagent container requiring temperature control is restricted. If a reagent container that does not require temperature control is placed in the holding hole of the sample holding portion in the holding hole of the container holding portion, it is not necessary to arrange a plurality of temperature controlling means.
【００１０】また、反応容器が、例えばフェライトや
鉄、マグネティックスターラピースを含む場合には、反
応容器保持部近傍に、反応容器に対して振動磁場を与え
るための磁石揺動手段を配置することが特に好ましい。When the reaction vessel contains, for example, ferrite, iron, or a magnetic stirrer piece, magnet swing means for applying an oscillating magnetic field to the reaction vessel may be arranged near the reaction vessel holding portion. Particularly preferred.
【００１１】[0011]
【発明の実施の形態】以下に、図面に基づき本発明を更
に詳細に説明する。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail with reference to the drawings.
【００１２】図１は、免疫化学分析装置の一部を構成す
る、本発明の容器保持装置の一例を示すものである。該
免疫化学分析装置においては、フェライトを練り込んだ
樹脂製の担体（抗原に対する抗体が固定されている）、
抗原に対する酵素標識抗体等の試薬が予め凍結乾燥され
て封入されている。図中、１は試料を収容する試料容
器、２は試料容器保持部、３は試料容器保持部に設けら
れた保持穴、４は反応容器、５は反応容器保持部、６は
反応容器保持部に設けられた保持穴、７は両保持部を連
結する連結手段、８は試薬容器であり、うち８ａは温調
が不要な凍結乾燥試薬の溶解液を収容する容器、８ｂは
温調が必要な酵素基質液を収容する容器、９、１０及び
１１は、それぞれ磁石揺動手段を構成するモータ、磁
石、磁石保持板をそれぞれ示す。FIG. 1 shows an example of the container holding device of the present invention, which constitutes a part of the immunochemical analyzer. In the immunochemical analyzer, a resin carrier into which ferrite is kneaded (an antibody to an antigen is immobilized);
Reagents such as an enzyme-labeled antibody against the antigen are freeze-dried and sealed in advance. In the figure, 1 is a sample container for accommodating a sample, 2 is a sample container holding unit, 3 is a holding hole provided in the sample container holding unit, 4 is a reaction container, 5 is a reaction container holding unit, and 6 is a reaction container holding unit. , A connecting means for connecting both holding portions, 8 a reagent container, 8a a container for containing a lyophilized reagent solution which does not require temperature control, and 8b a temperature control required The containers 9, 10, and 11 that accommodate the various enzyme substrate liquids respectively represent a motor, a magnet, and a magnet holding plate that constitute the magnet swing means.
【００１３】試料容器１は、ポリスチレン製のカップを
使用したが、この他にも、例えばポリプロピレン又はガ
ラス等の、試料溶液に接した場合に変質・変性しない材
質のもので構成されたカップ状のものや、通常使用され
る採血管のような管状のものが例示できる。保持穴３
は、図に示したように、試料容器保持部２上に１列に配
置される。保持穴の内径や深さ等は、保持される試料容
器１に基づいて適宜決定される。The sample container 1 used was a cup made of polystyrene. However, in addition to this, a cup-like material made of a material such as polypropylene or glass which does not deteriorate or denature when it comes into contact with the sample solution is used. And a tubular one such as a commonly used blood collection tube. Holding hole 3
Are arranged in a row on the sample container holder 2 as shown in the figure. The inner diameter and depth of the holding hole are appropriately determined based on the sample container 1 to be held.
【００１４】反応容器４は、ポリスチレン製のカップを
使用したが、例えばポリプロピレン又はガラス等の、反
応溶液に接した場合に変質・変性しない材質のもので構
成されたカップ状のものや、管状のものが例示できる
が、反応容器について温調を行い、又は後述の振動磁場
による攪拌を行う場合には、それぞれ熱伝導性が良好
で、かつ、磁力を透過し得る材質のものを用いる。反応
容器には、本例のように分析に必要な試薬の一部のみが
封入等されていても良いし、又は全部が予め封入等され
ていても良い。The reaction vessel 4 is made of a polystyrene cup. For example, a cup-shaped one made of a material such as polypropylene or glass which does not deteriorate or denature when exposed to a reaction solution, or a tubular one is used. In the case of controlling the temperature of the reaction vessel or performing stirring by an oscillating magnetic field described later, a material having good thermal conductivity and capable of transmitting magnetic force is used. In the reaction vessel, only a part of the reagent necessary for the analysis may be sealed or the like as in this example, or the whole may be previously sealed or the like.
【００１５】反応容器には、揺動磁場による攪拌を行う
ために、例えばフェライトや鉄等の常磁性、反強磁性物
質が入れられていても良い。例えば免疫化学分析の場合
は水不溶性の樹脂製担体を使用することがあるが、本例
のように、該担体中にフェライトを練り込む等して得ら
れたものの他に、磁石（スターラチップ）を使用するこ
とも例示できる。保持穴６は、図に示したように、反応
容器保持部５上に１列に配置される。保持穴の内径や深
さ等は、保持される反応容器４に基づいて適宜決定され
る。The reaction vessel may contain a paramagnetic or antiferromagnetic substance such as ferrite or iron in order to stir by a fluctuating magnetic field. For example, in the case of immunochemical analysis, a water-insoluble resin carrier may be used. In addition to a material obtained by kneading ferrite into the carrier as in this example, a magnet (stirrer chip) Can also be used. The holding holes 6 are arranged in a row on the reaction container holding portion 5 as shown in the figure. The inner diameter and depth of the holding hole are appropriately determined based on the reaction container 4 held.
【００１６】試料容器保持部２及び反応容器保持部５
は、それぞれ、アルミから構成されているが、試料容器
保持部２は、プラスチックや鉄等で構成することもでき
る。反応容器保持部５は、反応容器について温調を行
い、又は後述の振動磁場による攪拌を行う場合には、熱
伝導性が良好で、かつ、磁力透過性の材質であるアルミ
等を用いると共に、保持穴の内部が反応容器等の外周部
に密接して閉塞し、底面を薄くすることが好ましい。本
例では、反応容器保持部５の側面（不図示）に市販のヒ
ータを貼り付けるとともに、保持部本体には温度センサ
を取り付け、温度センサからの出力に応じてフィードバ
ック的に温調を行うようにしてある。また、反応容器保
持部の下部には、偏心配置された突起を有する円盤を取
り付けたモータ９と、該突起に嵌合され、モータの回転
により前後に揺動するＴ字型の磁石保持板１１と、磁石
保持板に配置された磁石１０からなる磁石揺動手段が取
り付けられている。磁石揺動手段は、本例のように、反
応容器保持部に取り付けても良いし、水平移動手段によ
り反応容器保持部が移動する経路の下部に僅かに離間し
て配置しても良い。Sample container holder 2 and reaction container holder 5
Are made of aluminum, respectively, but the sample container holding section 2 can be made of plastic, iron or the like. When the temperature of the reaction vessel is controlled for the reaction vessel, or when stirring is performed by an oscillating magnetic field described below, the reaction vessel holding section 5 has good thermal conductivity and uses aluminum or the like, which is a magnetically permeable material. It is preferable that the inside of the holding hole is closely closed to the outer peripheral portion of the reaction container or the like and the bottom surface is thinned. In this example, a commercially available heater is attached to a side surface (not shown) of the reaction container holding unit 5, a temperature sensor is attached to the holding unit main body, and the temperature is controlled in a feedback manner according to the output from the temperature sensor. It is. Further, a motor 9 having a disk having eccentrically arranged projections attached to a lower portion of the reaction vessel holding portion, and a T-shaped magnet holding plate 11 fitted to the projections and swinging back and forth by rotation of the motor. And a magnet swinging means comprising a magnet 10 arranged on a magnet holding plate. The magnet oscillating means may be attached to the reaction vessel holding part as in this example, or may be arranged slightly below the path along which the reaction vessel holding part moves by the horizontal moving means.
【００１７】試料容器保持部２と反応容器保持部５は、
連結手段７により両保持部上の保持穴の列が平行となる
ように連結される。ここで、両者の離間距離は０．５ｃ
ｍであり、不図示の温調手段を用いて反応容器保持部５
について温調を行った場合にも、介在する空気層により
試料容器中の試料にはその作用が及ばないようにしてあ
る。従って、試料の温度が上昇して蒸発したり、変性し
たりして、分析に悪影響を及ぼすことを排除できる。The sample container holder 2 and the reaction container holder 5 are
The connecting means 7 are connected so that the rows of holding holes on both holding parts are parallel. Here, the distance between the two is 0.5c
m, using a temperature control means (not shown)
Even when the temperature of the sample is adjusted, the action of the sample in the sample container is not affected by the intervening air layer. Therefore, it is possible to exclude that the temperature of the sample is increased and evaporated or denatured to adversely affect the analysis.
【００１８】このように、連結手段で連結された容器保
持部を水平方向に移動するには、図２に示したような水
平移動手段を用いる。該手段は、ガイドレール１２とガ
イドレールに沿って容器保持部を移動するためのパルス
モータ１３及びベルト、プーリとから構成され、ガイド
レール１２が貫通する貫通穴を有する不図示の台座を反
応容器保持部５に固定してある。In order to move the container holders connected by the connecting means in the horizontal direction, a horizontal moving means as shown in FIG. 2 is used. The means includes a guide rail 12, a pulse motor 13 for moving the container holding unit along the guide rail, a belt, and a pulley, and a base (not shown) having a through hole through which the guide rail 12 passes. It is fixed to the holding part 5.
【００１９】図１に示した装置では、分析を行うために
必要な試薬を収容する２種類の試薬容器８ａ及び８ｂ
を、それぞれ試料保持部又は反応容器保持部上に配置し
た、試薬容器用の保持穴に置くようにした。このよう
に、試薬容器の外径や形状等が各容器保持部の保持穴の
内径や形状等に合致しない場合には、使用する試薬容器
の外径や形状等に併せて、いずれか又は両容器保持部上
で１列に並んだ保持穴の延長線上に異なる外径、形状の
保持穴を追加する等すれば良いが、試料容器用又は反応
容器用の保持穴をそのまま使用することもできる。ま
た、特に多種類の試薬を使用する場合には、例えば試料
容器保持部又は反応容器保持部上に平行な２列の保持穴
を設け、うち一方の列を試料容器又は反応容器の保持の
ために、他方を試薬容器の保持のために使用することも
できる。更には、例えば試薬容器を保持するための１列
の保持穴を有する試薬容器保持部を新たに追加し、合計
３個の保持部を連結手段を用いて平行に連結しても良
い。かかる構成を採用する場合には、試料容器保持穴間
の離間距離、反応容器保持穴間の離間距離、試薬容器保
持穴間の離間距離、試薬容器保持穴と試料容器保持穴間
の離間距離、及び、試薬容器保持穴と反応容器保持穴間
の離間距離を同一にしておけば、水平移動手段による移
動操作を容易にすることが可能である。しかし、図に示
したように、試薬容器保持穴間の離間距離を、試薬容器
保持穴間、反応容器保持穴間の離間距離の１．５倍とす
る等、試薬容器の保持穴間の離間距離を、試薬容器保持
穴間、反応容器保持穴間の離間距離と異なるようにした
場合には、全ての容器について分注操作を行い得るよ
う、容器保持部の水平移動手段を調整すれば良い。In the apparatus shown in FIG. 1, two types of reagent containers 8a and 8b containing reagents necessary for performing an analysis are provided.
Was placed in a holding hole for a reagent container, which was placed on the sample holding portion or the reaction container holding portion, respectively. As described above, when the outer diameter or the shape of the reagent container does not match the inner diameter or the shape of the holding hole of each container holding portion, one or both of the outer diameters and the shape of the reagent container to be used are adjusted. The holding holes having different outer diameters and shapes may be added on the extension lines of the holding holes arranged in a line on the container holding portion, but the holding holes for the sample container or the reaction container may be used as they are. . In particular, when using various types of reagents, for example, two parallel rows of holding holes are provided on the sample container holding portion or the reaction container holding portion, and one of the rows is used for holding the sample container or the reaction container. Alternatively, the other can be used to hold a reagent container. Further, for example, a reagent container holding portion having a row of holding holes for holding the reagent containers may be newly added, and a total of three holding portions may be connected in parallel using the connecting means. When such a configuration is adopted, the separation distance between the sample container holding holes, the separation distance between the reaction container holding holes, the separation distance between the reagent container holding holes, the separation distance between the reagent container holding holes and the sample container holding holes, If the distance between the reagent container holding hole and the reaction container holding hole is the same, the moving operation by the horizontal moving means can be facilitated. However, as shown in the figure, the distance between the reagent container holding holes is set to 1.5 times the distance between the reagent container holding holes and the distance between the reaction container holding holes. When the distance is different from the distance between the reagent container holding holes and the distance between the reaction container holding holes, the horizontal moving means of the container holding unit may be adjusted so that the dispensing operation can be performed for all the containers. .
【００２０】本例では、前述のように、温調が不要な凍
結乾燥試薬の溶解液を収容する容器８ａ、温調が必要な
酵素基質液を収容する容器８ｂを用いたが、これらの他
に、温調が不要な試薬として試料を希釈するための希釈
液やいわゆる第２試薬（酵素標識抗体）を例示すること
ができる。In this embodiment, as described above, the container 8a for storing the lyophilized reagent solution which does not need to be temperature-controlled and the container 8b for storing the enzyme substrate solution which needs to be temperature-controlled are used. Examples of the reagent that does not require temperature control include a diluent for diluting a sample and a so-called second reagent (enzyme-labeled antibody).
【００２１】本発明の容器保持装置は、分注装置等と機
能的に一体に構成され、全体として免疫化学分析を構成
する。図２は、かかる免疫化学分析装置の概略を示すも
のである。The container holding device of the present invention is functionally integrated with a pipetting device and the like, and constitutes an immunochemical analysis as a whole. FIG. 2 schematically shows such an immunochemical analyzer.
【００２２】図２中、１４は分注装置のノズル、１５は
ノズルを上下及び水平方向（図中に矢印で示した、容器
保持装置の水平移動方向に垂直な方向）に移動可能に支
持するノズル支持手段、１６はノズル先端に取り付けら
れるチップを介して液体を吸引・吐出するためのポンプ
手段、１７はノズルに対して取り付けられるべきチップ
を供給するチップ供給手段である。本例では、試料間の
汚染を防止する目的等で使い捨てチップを使用する構成
であり、このため、使用済みのチップを廃棄するための
廃棄箱１８を備えている。In FIG. 2, reference numeral 14 denotes a nozzle of the dispensing device, and reference numeral 15 denotes a nozzle which supports the nozzle so as to be movable in the vertical and horizontal directions (directions indicated by arrows in the drawing, which are perpendicular to the horizontal movement direction of the container holding device). Nozzle support means, 16 is a pump means for sucking / discharging the liquid through a tip attached to the tip of the nozzle, and 17 is a tip supply means for supplying a tip to be attached to the nozzle. In this example, a disposable tip is used for the purpose of preventing contamination between samples and the like, and therefore, a disposal box 18 for discarding a used tip is provided.
【００２３】また、免疫化学分析を実施するために、本
例の装置は、抗原等と免疫複合体を形成していない標識
試薬を除去するための、いわゆるＢ／Ｆ分離手段１９、
免疫複合体を形成している標識試薬量を検出するための
検出手段２０を装備している。Ｂ／Ｆ分離手段１９は、
洗浄液容器２１及び排出液溜２２に接続されている。In order to carry out an immunochemical analysis, the apparatus of this embodiment is provided with a so-called B / F separation means 19 for removing a labeling reagent which has not formed an immunocomplex with an antigen or the like.
A detection means 20 for detecting the amount of the labeling reagent forming the immune complex is provided. The B / F separation means 19
It is connected to a cleaning liquid container 21 and a discharge liquid reservoir 22.
【００２４】なお本例の分析装置は、二段構造を有して
おり、第１の平面には前記ノズル支持手段１５、チップ
供給手段１７、Ｂ／Ｆ分離手段１９及び検出手段２０が
配置され、該平面の下部に設けられた第２の平面上に本
発明の容器保持装置と廃棄箱１８が配置されている。第
１の平面には、第２の平面との連絡穴Ａ及びＢが設けら
れ、該穴を通してノズル１４を下降させることで、試料
容器又は試薬容器（穴Ａ）或いは反応容器又は試薬容器
（穴Ｂ）に収容された試料等を吸引等することができ
る。The analyzer of this embodiment has a two-stage structure, and the nozzle support means 15, chip supply means 17, B / F separation means 19 and detection means 20 are arranged on a first plane. The container holding device of the present invention and the disposal box 18 are arranged on a second plane provided below the plane. The first plane is provided with communication holes A and B with the second plane, and by lowering the nozzle 14 through the holes, a sample container or a reagent container (hole A) or a reaction container or a reagent container (hole) is formed. The sample or the like stored in B) can be sucked or the like.
【００２５】[0025]
【発明の効果】本発明の容器保持装置によれば、試料容
器、反応容器そして場合によっては試薬容器を、熱的に
は遮断されているが一体として移動できる１台の容器保
持装置で保持するため、容器保持装置としても簡便で小
型であるが、これを免疫化学分析装置等の分析装置に配
置した場合には当該分析装置をも小型にできるという効
果がある。しかも、保持された容器に収容された試料等
を吸引・吐出する場合には、分注装置を保持装置の水平
移動方向に垂直に移動させるのみで良いため、その移動
手段を簡便化、小型化できるという効果もある。According to the container holding device of the present invention, the sample container, the reaction container and, in some cases, the reagent container are held by one container holding device which is thermally insulated but can be moved integrally. For this reason, the container holding device is simple and compact, but when it is arranged in an analyzer such as an immunochemical analyzer, there is an effect that the analyzer can be downsized. In addition, when aspirating and discharging a sample or the like stored in the held container, it is only necessary to move the dispensing device perpendicularly to the horizontal movement direction of the holding device, so that the moving means is simplified and downsized. There is also an effect that can be done.
【００２６】また前述のように、仮に温調が必要な試薬
と不要な試薬の両方を使用する場合であっても、単一の
温調手段を配置しておくのみで済むという効果もある。Further, as described above, even if both a reagent requiring temperature control and an unnecessary reagent are used, there is an effect that only a single temperature control means is required.
【図１】図１は、本発明の容器保持装置の概略を説明す
るための図である。FIG. 1 is a diagram for explaining an outline of a container holding device of the present invention.
【図２】図２は、本発明の容器保持装置を具備する、免
疫化学分析装置の概略について説明をするための図であ
る。FIG. 2 is a diagram for explaining an outline of an immunochemical analyzer provided with the container holding device of the present invention.
１ 試料容器 ２ 試料容器保持部 ３ 試料容器保持部に設けられた保持穴 ４ 反応容器 ５ 反応容器保持部 ６ 反応容器保持部に設けられた保持穴 ７ 連結手段 ８ 試薬容器 ９ モータ（磁石揺動手段） １０ 磁石 １１ 磁石保持板（磁石揺動手段） １２ ガイドレール １３ パルスモータ １４ ノズル（分注装置） １５ ノズル支持手段（分注装置） １６ ポンプ手段（分注装置） １７ チップ供給装置 １８ 使用済チップ廃棄箱 １９ Ｂ／Ｆ分離手段 ２０ 検出手段 ２１ 洗浄液容器 ２２ 廃液溜 DESCRIPTION OF SYMBOLS 1 Sample container 2 Sample container holding part 3 Holding hole provided in sample container holding part 4 Reaction container 5 Reaction container holding part 6 Holding hole provided in reaction container holding part 7 Connecting means 8 Reagent container 9 Motor (magnet swing Means) 10 Magnet 11 Magnet holding plate (magnet swinging means) 12 Guide rail 13 Pulse motor 14 Nozzle (dispensing device) 15 Nozzle support means (dispensing device) 16 Pump means (dispensing device) 17 Chip supply device 18 Use Used chip disposal box 19 B / F separation means 20 detection means 21 cleaning liquid container 22 waste liquid storage
Claims (6)
１列の保持穴を有する試料容器保持部と、反応用の反応
容器を保持するための１列の保持穴を有する反応容器保
持部と、これら保持部の保持穴が平行となるように両保
持部を連結する連結手段と、連結された両保持部を保持
穴の列方向に水平移動するための水平移動手段とからな
る、容器保持装置。1. A sample container holder having a single row of holding holes for holding a sample container containing a sample, and a reaction container holder having a single row of holding holes for holding a reaction container for a reaction. A container comprising: connecting means for connecting the two holding parts such that the holding holes of the holding parts are parallel; and horizontal moving means for horizontally moving the connected two holding parts in the row direction of the holding holes. Holding device.
適当な温度範囲に温調するための温調手段を有すると共
に、試料容器保持部が該温調手段の温調作用から熱的に
遮断されていることを特徴とする請求項１の容器保持装
置。2. The temperature control means for controlling the temperature of the reaction vessel holding portion to a temperature range suitable for causing a reaction, and the sample container holding portion is thermally controlled by the temperature controlling action of the temperature controlling means. 2. The container holding device according to claim 1, wherein the container holding device is closed.
間距離と反応容器保持部に設けられた保持穴間の離間距
離が共に一定かつ同一であり、水平移動手段が１回の作
動により該離間距離分又は離間距離の整数倍、両保持部
を水平移動し得るものであることを特徴とする請求項１
の容器保持装置。3. The distance between the holding holes provided in the sample container holding part and the distance between the holding holes provided in the reaction container holding part are both constant and the same, and the horizontal moving means operates once. 2. The two holders can be horizontally moved by the distance or an integral multiple of the distance.
Container holding device.
容器保持部に設けられた保持穴が、これら保持部の水平
移動方向に垂直な直線上に位置するように連結手段によ
り連結されていることを特徴とする請求項３の容器保持
装置。4. A holding means provided in the sample container holding part and a holding hole provided in the reaction container holding part are connected by connecting means so as to be located on a straight line perpendicular to the horizontal movement direction of the holding part. 4. The container holding device according to claim 3, wherein:
に設けられた保持穴の一部が、反応に必要な試薬を収容
する試薬容器を保持するための保持穴として使用される
ことを特徴とする請求項１の容器保持装置。5. A method according to claim 5, wherein a part of the holding hole provided in the sample container holding part and / or the reaction container holding part is used as a holding hole for holding a reagent container containing a reagent necessary for the reaction. The container holding device according to claim 1, wherein
振動磁場を与えるための磁石揺動手段を有する、請求項
１の容器保持装置。6. The container holding device according to claim 1, further comprising a magnet swinging means for applying an oscillating magnetic field to the reaction container near the reaction container holding portion.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9125368A JPH10319023A (en) | 1997-05-15 | 1997-05-15 | Vessel holding device |
| FR9806199A FR2763396A1 (en) | 1997-05-15 | 1998-05-15 | DOSING APPARATUS, AND CONTAINER SUPPORT DEVICE FOR USE WITH SAID APPARATUS |
| DE19821935A DE19821935A1 (en) | 1997-05-15 | 1998-05-15 | Immunoanalysis apparatus |
| US09/079,184 US5904899A (en) | 1997-05-15 | 1998-05-15 | Assaying apparatus and a vessel holder device in use with the assaying apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9125368A JPH10319023A (en) | 1997-05-15 | 1997-05-15 | Vessel holding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10319023A true JPH10319023A (en) | 1998-12-04 |
Family
ID=14908409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9125368A Pending JPH10319023A (en) | 1997-05-15 | 1997-05-15 | Vessel holding device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10319023A (en) |
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| JP5319672B2 (en) * | 2008-06-12 | 2013-10-16 | 株式会社日立ハイテクノロジーズ | Analyzer using magnetic particles |
| JP2015111150A (en) * | 2008-12-25 | 2015-06-18 | ユニバーサル・バイオ・リサーチ株式会社 | Method for pretreating specimen, and method for assaying bio-related substance |
| CN114207434A (en) * | 2019-07-29 | 2022-03-18 | 沃特世科技公司 | Method of loading a sample into a sample manager of a chromatography system |
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