JPH10277586A - Purifying agent using microorganism - Google Patents
Purifying agent using microorganismInfo
- Publication number
- JPH10277586A JPH10277586A JP9057697A JP9057697A JPH10277586A JP H10277586 A JPH10277586 A JP H10277586A JP 9057697 A JP9057697 A JP 9057697A JP 9057697 A JP9057697 A JP 9057697A JP H10277586 A JPH10277586 A JP H10277586A
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- JP
- Japan
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- weight
- parts
- purifying agent
- microorganisms
- zeolite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、汚水の浄化剤に関
するものであり、更には、本発明は、凝集沈澱効果を利
用して汚水をきれいにする浄化剤に関する。TECHNICAL FIELD The present invention relates to a purifying agent for sewage, and more particularly, to a purifying agent for purifying sewage by utilizing a coagulating sedimentation effect.
【０００２】[0002]
【従来の技術】近年、池や沼、用水等が汚染されている
場合が多く、このような池や沼、用水等の汚水の浄化
は、従来ゼオライト、木炭、その他多孔質体を汚水中に
設置して汚水中に分布している微生物を固定することに
より汚水を浄化する方法が試みられており、また汚水の
浄化を腐植物質を成分とする浄化剤を使用して汚水中に
分布している微生物を活性化し、凝集沈降効果を利用し
て浄化する方法があり、例えば特開平５−３１４９０号
公報には、フルボ酸、腐植酸等の腐植植物、粘土鉱物及
び活性鉄等を含む腐植質鉄錯体を汚水中に投入し、酸化
反応と微生物群の増殖を行って、これを曝気工程で浄化
することが開示されている。2. Description of the Related Art In recent years, ponds, swamps, irrigation water and the like are often contaminated. Purification of sewage such as ponds, swamps and irrigation water has conventionally been carried out by converting zeolite, charcoal, and other porous materials into sewage. Attempts have been made to purify sewage by fixing microorganisms distributed in the sewage by installing the sewage. There is a method of activating microorganisms and purifying them by utilizing the coagulation sedimentation effect. For example, JP-A-5-31490 discloses a humic plant containing humic acid such as fulvic acid and humic acid, a clay mineral, and active iron. It is disclosed that an iron complex is put into wastewater, an oxidation reaction and a growth of microorganisms are carried out, and this is purified in an aeration step.
【０００３】[0003]
【発明が解決しようとする課題】しかしながら、前述の
如くゼオライト、木炭、その他多孔質体を汚水中に設置
して汚水中に分布している微生物を固定することにより
汚水を浄化する方法は、一応の生物効果は示すものの即
効性に乏しく、効率の悪いものであった。また腐植物質
を成分とする浄化剤を使用する方法もまた即効性に問題
があり、現場にセットしてから、略嫌気的な処理を行う
ことが決め手となるため運転が難しく、処理を効果的に
行うには経験乃至熟練が必要であった。更に腐植質鉄錯
体を使用する方法では、曝気工程を使用する点で浄化が
複雑かつ経費がかかる等の問題がある。However, as described above, a method for purifying sewage by installing zeolites, charcoal, and other porous materials in sewage and fixing microorganisms distributed in the sewage is as follows. Although its biological effect was shown, it was poor in immediate effect and inefficient. In addition, the method of using a purifying agent containing humic substances also has a problem in the immediate effect, and it is difficult to operate the apparatus because it is crucial to perform an anaerobic treatment after setting it on the site, so that the operation is difficult and the treatment is effective. Requires experience or skill. Further, the method using the humic iron complex has a problem that purification is complicated and costly in that an aeration step is used.
【０００４】そこで、本発明等は、前述の如き問題点に
ついて種々検討したところ、微生物群を積極的に繁殖さ
せて使用することにより、前述如き問題点を解決するこ
とができ、即効性に優れ、十分な浄化効果を有し、運転
操作が容易であることを見出し、この知見に基づいて本
発明はなされたものである。したがって、本発明が解決
しようとする課題は、即効性に優れ、十分な浄化効果を
有し、運転操作が容易である浄化剤を提供することにあ
る。Therefore, the present invention and the like have made various studies on the above-mentioned problems, and can solve the above-mentioned problems by actively breeding the microorganisms and using them. The present invention has been found to have a sufficient purifying effect and to facilitate driving operation, and to be based on this finding. Therefore, an object of the present invention is to provide a purifying agent which is excellent in immediate effect, has a sufficient purifying effect, and is easy to operate.
【０００５】[0005]
【課題を解決するための手段】本発明の上記課題は、以
下の各発明によって達成される。The above objects of the present invention are attained by the following inventions.
【０００６】（１）微生物群、腐植物質、フミン酸、フ
ルボ酸、ゼオライト、糖類及び窒素系栄養源からなる浄
化剤。 （２）割合が微生物群０．０５〜０．３重量部、腐植物
質０．５〜３重量部、フミン酸０．１〜１．０重量部、
フルボ酸０．１〜１．０、ゼオライト０．１〜１０重量
部、糖類（糖質）０．０５〜５重量部、窒素系栄養源
０．０１〜２重量部からなることを特徴とする前記第１
項に記載の浄化剤。 （３）前記第２項に記載の浄化剤に水５〜３０重量部を
混合し、温度２８℃〜４０℃、酸素供給量０．０３リッ
トル／分〜０．１０リットル／分で３６時間培養した
後、更に継続して温度２８℃〜４０℃、酸素供給を行わ
ないで６０時間培養した培養物を凍結乾燥した粉末であ
ることを特徴とする浄化剤。 （４）微生物群がバチルス属、ストレプトコッカス属、
エンテロバクター属、リゾープス属、ニトロソモナス
属、ニトロバクター属、シュードモナス属及びセルロモ
ナス属を含むことを特徴とする前記第１項乃至第３項の
いずれかに記載の浄化剤。 （５）微生物群１０重量部に対し、糖類５〜１０重量
部、窒素系栄養源０．２〜５重量部、珪酸カルシウムの
多孔体２０〜１００重量部、水１０００〜５０００重量
部の混合物を、温度２８℃〜４０℃、酸素供給量０．０
３リットル／分〜０．１０リットル／分で３６時間培養
することによって得られた培養物を凍結乾燥した粉末で
あることを特徴とする前記第１項又は第４項に記載の浄
化剤。 （６）ゼオライトが、沸石又は海緑石を含有しているこ
とを特徴とする前記第１項乃至第５項のいずれかに記載
の浄化剤。(1) A purifying agent comprising a group of microorganisms, humic substances, humic acid, fulvic acid, zeolite, saccharides, and nitrogen-based nutrients. (2) a ratio of microorganisms of 0.05 to 0.3 parts by weight, humic substances 0.5 to 3 parts by weight, humic acid 0.1 to 1.0 parts by weight,
It is characterized by comprising 0.1 to 1.0 parts by weight of fulvic acid, 0.1 to 10 parts by weight of zeolite, 0.05 to 5 parts by weight of saccharide (saccharide), and 0.01 to 2 parts by weight of a nitrogen-based nutrient. The first
The purifying agent according to Item. (3) 5 to 30 parts by weight of water is mixed with the purifying agent described in the above item 2, and the mixture is cultured for 36 hours at a temperature of 28 ° C to 40 ° C and an oxygen supply amount of 0.03 l / min to 0.10 l / min. A purifying agent, characterized in that it is a freeze-dried powder of a culture cultured at a temperature of 28 ° C. to 40 ° C. for 60 hours without supplying oxygen after being further continued. (4) the microorganism group is Bacillus, Streptococcus,
4. The purifying agent according to any one of the above-mentioned items 1 to 3, comprising Enterobacter, Rhizopus, Nitrosomonas, Nitrobacter, Pseudomonas and Cellulomonas. (5) A mixture of 5 to 10 parts by weight of a saccharide, 0.2 to 5 parts by weight of a nitrogen-based nutrient, 20 to 100 parts by weight of a porous body of calcium silicate, and 1000 to 5000 parts by weight of water is added to 10 parts by weight of a microorganism group. , Temperature 28 ° C-40 ° C, oxygen supply 0.0
5. The purifying agent according to the above item 1 or 4, which is a freeze-dried powder of a culture obtained by culturing at a rate of 3 l / min to 0.10 l / min for 36 hours. (6) The purifying agent according to any one of (1) to (5) above, wherein the zeolite contains zeolite or chlorite.
【０００７】[0007]
【発明の実施の形態】以下に、本発明を更に詳しく説明
する。本発明の浄化剤は、微生物群、腐植物質、フミン
酸、フルボ酸、ゼオライト、糖類及び窒素系栄養源から
なることにより、即効性に優れ、十分な浄化効果を有
し、運転操作が容易であるという効果を奏するものであ
る。また本発明の浄化剤は、その割合が微生物群０．０
５〜０．３重量部、腐植物質０．５〜３重量部、フミン
酸０．１〜１．０重量部、フルボ酸０．１〜１．０、ゼ
オライト０．１〜１０重量部、糖類（糖質）０．０５〜
５重量部、窒素系栄養源０．０１〜２重量部からなるこ
とにより、好ましい前記効果を奏するものである。BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail. The purifying agent of the present invention is excellent in immediate action, has a sufficient purifying effect, and is easy to operate by being composed of microorganisms, humic substances, humic acid, fulvic acid, zeolite, saccharides and nitrogen-based nutrients. There is an effect that there is. The purifying agent of the present invention has a ratio of the microorganism group 0.0
5 to 0.3 parts by weight, humic substances 0.5 to 3 parts by weight, humic acid 0.1 to 1.0 part by weight, fulvic acid 0.1 to 1.0, zeolite 0.1 to 10 parts by weight, sugar (Sugar) 0.05-
The preferred effects are exhibited by comprising 5 parts by weight and 0.01 to 2 parts by weight of a nitrogen-based nutrient source.
【０００８】本発明では、前記割合で混合されている浄
化剤に水５〜３０重量部を混合し、温度２８℃〜４０
℃、酸素供給量０．０３リットル／分〜０．１０リット
ル／分で３６時間培養した後、更に継続して温度３０
℃、酸素供給を行わないで６０時間培養し、得られた培
養物を凍結乾燥して浄化剤粉末を得ることにより、好気
性微生物を多量に増殖することができると共に嫌気性微
生物の増殖も極力増殖させることができ、両者の拮抗作
用の少ない状態にすることができるもので、両微生物群
に富んだ優れた浄化剤が得られ、しかも取り扱いが容易
である。In the present invention, 5 to 30 parts by weight of water is mixed with the purifying agent mixed in the above ratio, and the temperature is 28 ° C. to 40 ° C.
C. for 36 hours at an oxygen supply rate of 0.03 liter / min to 0.10 liter / min.
Cultivation at 60 ° C. for 60 hours without supplying oxygen, and freeze-drying of the obtained culture to obtain a purifying agent powder enables a large amount of aerobic microorganisms to grow and also the growth of anaerobic microorganisms to the utmost. It can be grown and can be in a state with little antagonistic action between them, and an excellent purifying agent rich in both microorganism groups can be obtained, and it is easy to handle.
【０００９】更に、本発明において、微生物群がバチル
ス属、ストレプトコッカス属、エンテロバクター属、リ
ゾープス属、ニトロソモナス属、ニトロバクター属、シ
ュードモナス属及びセルロモナス属を含むことにより、
浄化剤の性能が優れ、即ち即効性に優れ、十分な浄化効
果を有する浄化剤が得られる。Furthermore, in the present invention, the microorganisms include Bacillus, Streptococcus, Enterobacter, Rhizopus, Nitrosomonas, Nitrobacter, Pseudomonas, and Cellulomonas.
A purifying agent having excellent purifying performance, that is, excellent immediate effect, and having a sufficient purifying effect can be obtained.
【００１０】更に本発明では、前記の第１項〜第４項ま
での浄化剤において、微生物群１０重量部に対し、糖類
５〜１０重量部、窒素系栄養源０．２〜５重量部、珪酸
カルシウムの多孔体２０〜１００重量部、水１０００〜
５０００重量部の混合物を、温度２０℃〜４０℃、酸素
供給量０．０３リットル／分〜０．１０リットル／分で
３６時間培養することによって得られた培養物を凍結乾
燥した粉末であることにより、好気性の微生物群に富ん
だ優れた浄化剤が得られ、しかも取り扱いが容易であ
る。In the present invention, the purifying agent according to any one of the above-mentioned items 1 to 4, wherein 5 to 10 parts by weight of saccharides, 0.2 to 5 parts by weight of a nitrogen-based nutrient, 20 to 100 parts by weight of a porous material of calcium silicate,
A freeze-dried powder of a culture obtained by culturing 5000 parts by weight of a mixture at a temperature of 20 ° C. to 40 ° C. and an oxygen supply of 0.03 liter / min to 0.10 liter / min for 36 hours. Thereby, an excellent purifying agent rich in aerobic microorganisms can be obtained, and the handling is easy.
【００１１】本発明において、ゼオライトが、沸石又は
海緑石を含有していることにより微生物群の固定を良好
にすることができる。In the present invention, since the zeolite contains zeolite or chlorite, the fixation of microorganisms can be improved.
【００１２】本発明に用いられる浄化剤は、微生物群、
腐植物質、フミン酸、フルボ酸、ゼオライト、糖類及び
窒素系栄養源からなり、これらの各成分について説明す
ると、（１）本発明に用いられる微生物群としては、
バチルス（Ｂａｃｉｌｌｕｓ）属、ストレプトコッカ
ス（Ｓｔｒｅｐｔｏｃｏｃｃｕｓ）属、エンテロバク
ター（Ｅｎｔｅｒｏｂａｃｔｅｒ）属、リゾープス
（Ｒｈｉｚｏｐｕｓ）属、ニトロソモナス（Ｎｉｔｒ
ｏｓｏｍｏｎａｓ）属、ニトロバクター（Ｎｉｔｒｏ
ｂａｃｔｅｒ）属、シュードモナス（Ｐｓｅｕｄｏｍ
ｏｎａｓ）属及びセルロモナス（Ｃｅｌｌｕｌｏｍｏ
ｎａｓ）属を含むものから構成される。The purifying agent used in the present invention includes a microorganism group,
It consists of humic substances, humic acid, fulvic acid, zeolite, saccharides, and nitrogen-based nutrients. Each of these components will be described. (1) The microorganisms used in the present invention include:
Bacillus genus, Streptococcus genus, Enterobacter genus, Rhizopus genus, Nitrosomonas (Nitr)
osmonas, Nitrobacter (Nitro)
bacter), Pseudomonas
onas) and Cellulomonas (Cellulomo)
nas).
【００１３】このうち、〜の微生物は汚染源である
有機物の分解に寄与する。〜の微生物は汚染源から
発生するアンモニアの分解に寄与するものであり、は
アンモニア→ＮＯ2 、はＮＯ2 →ＮＯ3 、はＮＯ3
→Ｎ2 への変換を行ない、悪臭を無臭化する。また、
は糖類やアルコールを乳酸やクエン酸などの有機酸に
変換したり、酵素（アミラーゼ、プロテアーゼ、リパー
ゼなど）を産出し、汚染源（有機物）を分解する役割を
演ずる。はセルロースの分解に寄与する。以上、〜
の微生物がそれぞれの機能を果すことによって汚染源
の成分を分解・溶解する。Among them, the microorganisms (1) to (3) contribute to the decomposition of organic substances which are pollutants. Are microorganisms that contribute to the decomposition of ammonia generated from a pollution source, where ammonia → NO 2 , NO 2 → NO 3 , and NO 3
→ Converts to N 2 to eliminate odors. Also,
Plays a role in converting sugars and alcohols into organic acids such as lactic acid and citric acid, producing enzymes (amylase, protease, lipase, etc.) and decomposing pollutants (organic substances). Contributes to the decomposition of cellulose. that's all,~
Microorganisms perform their respective functions to decompose and dissolve the components of the pollution source.
【００１４】（２）腐植物質としては、天然の土壌から
なるもので、土壌の粗粒分を除去したものが用いられ
る。（３）フミン酸は、腐植物質から分離したもので、
汚水のｐＨを低下させアルカリ汚濁物の中和に寄与す
る。（４）フルボ酸は、腐植物質から分離したもので、
微生物を活性化させる作用を有する。また（５）ゼオラ
イトとしては、特に限定されるものではないが、好まし
くは沸石又は海緑石が挙げられ、これは担体として用い
られ、これを含有していることにより微生物群の固定を
良好にすることができる。（６）糖類は、特に限定され
るものでないが、グルコースが好ましい。グルコース
は、微生物の栄養源となり、微生物の生育を効果的に促
進する。更に（７）窒素系栄養源は、窒素を含む化合物
であれば、特に制限されないが、通常塩化アンモニウ
ム、硫安が用いられる。(2) As humic substances, those made of natural soil from which coarse particles of the soil have been removed are used. (3) Humic acid is separated from humic substances,
It lowers the pH of wastewater and contributes to neutralization of alkaline pollutants. (4) Fulvic acid is isolated from humic substances,
It has the effect of activating microorganisms. The (5) zeolite is not particularly limited, but preferably includes zeolite or chlorite, which is used as a carrier and contains the zeolite to improve the fixation of microorganisms. can do. (6) The saccharide is not particularly limited, but glucose is preferred. Glucose becomes a nutrient source for microorganisms and effectively promotes the growth of microorganisms. Further, (7) the nitrogen-based nutrient is not particularly limited as long as it is a compound containing nitrogen, but usually ammonium chloride and ammonium sulfate are used.
【００１５】本発明において、浄化剤の成分の割合は微
生物群０．０５〜０．３重量部、腐植物質０．５〜３重
量部、フミン酸０．１〜１．０重量部、フルボ酸０．１
〜１．０、ゼオライト０．１〜１０重量部、糖類（糖
質）０．０５〜５重量部、窒素系栄養源０．０１〜２重
量部からなるが、好ましくは微生物群０．１０〜０．３
重量部、腐植物質０．１〜３重量部、フミン酸０．３〜
１．０重量部、フルボ酸０．３〜１．０、ゼオライト１
〜８重量部、糖類（糖質）０．１〜５重量部、窒素系栄
養源０．１〜２重量部からなるのがよい。In the present invention, the ratio of the components of the purifying agent is 0.05 to 0.3 parts by weight of a microorganism group, 0.5 to 3 parts by weight of humic substances, 0.1 to 1.0 part by weight of humic acid, and fulvic acid. 0.1
0.1 to 10 parts by weight of zeolite, 0.05 to 5 parts by weight of saccharide (saccharide), 0.01 to 2 parts by weight of nitrogen-based nutrients, preferably 0.10 to 0.10 parts by microorganism group. 0.3
Parts by weight, humic substances 0.1-3 parts by weight, humic acid 0.3-
1.0 parts by weight, fulvic acid 0.3-1.0, zeolite 1
-8 parts by weight, 0.1-5 parts by weight of saccharide (saccharide), and 0.1-2 parts by weight of a nitrogen-based nutrient.
【００１６】これらの割合の場合は、微生物群０．０５
重量部未満では、即効性に乏しく、また０．３重量部を
越えると菌のバランスが崩れるので好ましくない。腐植
物質の割合が０．５重量部未満では、長期的な浄化に寄
与しない。また３重量部を越えると、生物活性に悪影響
を与える。フミン酸０．１重量部未満では、アルカリ汚
濁物を処理できない。また１．０重量部を越えてもそれ
なりまたはそれ以上の効果を示さない。またフルボ酸
０．１重量部未満では、生物活性を高めることができな
い。また１．０重量部を越えても、それなりの生物活性
の向上効果を示さない。ゼオライト０．１重量部未満で
は、生物の担持体として面積が不十分であり、また１０
重量部を越えても、担持能力に大した差がない。更に糖
類（糖質）０．０５重量部未満では、微生物の生育を効
果的に促進しない。また糖類が５重量部を越えるとバチ
ルス属やシュートモナス属の菌数が増えすぎて他種の菌
との共存が不可能となる。窒素系栄養源０．０１重量部
未満では、微生物の生育を効果的に促進しないし、また
２重量部を越えると菌のバランスが一定値以上にならな
い。In the case of these ratios, the microorganism group 0.05
If the amount is less than part by weight, the immediate effect is poor, and if it exceeds 0.3 part by weight, the balance of the bacteria is undesirably lost. If the ratio of humic substances is less than 0.5 parts by weight, it does not contribute to long-term purification. If it exceeds 3 parts by weight, the biological activity is adversely affected. If the humic acid content is less than 0.1 part by weight, the alkali pollutants cannot be treated. If the amount exceeds 1.0 part by weight, no significant effect is exhibited. If the amount of fulvic acid is less than 0.1 part by weight, the biological activity cannot be increased. If the amount exceeds 1.0 part by weight, no significant improvement in biological activity is exhibited. If the amount of zeolite is less than 0.1 part by weight, the area of the support for living organisms is insufficient, and
Even when the amount exceeds the weight part, there is no significant difference in the carrying capacity. Further, when the saccharide (saccharide) is less than 0.05 parts by weight, the growth of microorganisms is not effectively promoted. On the other hand, if the amount of the saccharide exceeds 5 parts by weight, the number of bacteria of the genus Bacillus or the genus Shootmonas increases too much, and coexistence with other species of bacteria becomes impossible. If the nitrogen-based nutrient source is less than 0.01 part by weight, the growth of microorganisms is not effectively promoted, and if it exceeds 2 parts by weight, the balance of the bacteria does not exceed a certain value.
【００１７】更に本発明では、微生物群０．０５〜０．
３重量部、腐植物質０．５〜３重量部、フミン酸０．１
〜１．０重量部、フルボ酸０．１〜１．０、ゼオライト
０．１〜１０重量部、糖類（糖質）０．０５〜５重量
部、窒素系栄養源０．０１〜２重量部からなる浄化剤に
水１０〜２５重量部を混合し、温度３０℃、酸素供給量
０．０５リットル／分で３６時間培養した後、更に継続
して温度３０℃、酸素供給を行わないで６０時間培養し
た培養物を凍結乾燥して浄化剤粉末を製造することによ
り、好気性微生物を多量に増殖することができると共に
嫌気性微生物の増殖も極力増殖させることができ、両者
の拮抗作用の少ない状態にすることができるもので、両
微生物群に富んだ優れた浄化剤が得られ、しかも取り扱
いが容易である。Further, in the present invention, the microorganism group 0.05 to 0.5.
3 parts by weight, humic substances 0.5 to 3 parts by weight, humic acid 0.1
1.0 to 1.0 parts by weight, fulvic acid 0.1 to 1.0, zeolite 0.1 to 10 parts by weight, saccharide (saccharide) 0.05 to 5 parts by weight, nitrogen-based nutrient source 0.01 to 2 parts by weight Was mixed with 10 to 25 parts by weight of water and cultured at a temperature of 30 ° C. and an oxygen supply rate of 0.05 l / min for 36 hours, and further continuously at a temperature of 30 ° C. without supplying oxygen. By freeze-drying the culture cultured for a period of time to produce a purifying agent powder, it is possible to grow a large amount of aerobic microorganisms and also to grow anaerobic microorganisms as much as possible, and there is little antagonistic action between the two. It can be put into a state, and an excellent purifying agent rich in both microbial groups can be obtained, and it is easy to handle.
【００１８】この浄化剤の割合は、好ましくは微生物群
０．０７〜０．２重量部、腐植物質０．５〜２重量部、
フミン酸０．３〜０．８重量部、フルボ酸０．３〜０．
８、ゼオライト０．５〜８重量部、糖類（糖質）０．０
７〜２重量部、窒素系栄養源０．０１〜１．５重量部か
らなる浄化剤に水１０〜２５重量部を混合し、温度３０
℃、酸素供給量０．０５リットル／分で３６時間培養し
た後、更に継続して温度３０℃、酸素供給を行わないで
６０時間培養した培養物を凍結乾燥して浄化剤粉末を製
造することがよく、この範囲で好気性微生物と嫌気性微
生物の両微生物群に富んだ最良の優れた浄化剤が得られ
る。本発明で使用する微生物群は、更に詳しくは以下の
表１の如くである。The ratio of the purifying agent is preferably 0.07 to 0.2 parts by weight of microorganisms, 0.5 to 2 parts by weight of humic substances,
Humic acid 0.3-0.8 parts by weight, fulvic acid 0.3-0.
8, 0.5 to 8 parts by weight of zeolite, saccharide (sugar) 0.0
10 to 25 parts by weight of water was mixed with a purifying agent consisting of 7 to 2 parts by weight and 0.01 to 1.5 parts by weight of a nitrogen-based nutrient,
After culturing for 36 hours at a temperature of 30 ° C. and an oxygen supply rate of 0.05 l / min, freeze-drying the culture further cultivated at a temperature of 30 ° C. for 60 hours without supplying oxygen to produce a purifying agent powder. In this range, the best and excellent purifying agent rich in both aerobic microorganisms and anaerobic microorganisms can be obtained. The microorganisms used in the present invention are shown in more detail in Table 1 below.
【００１９】[0019]
【表１】 [Table 1]
【００２０】更に本発明では、前記の第１項〜第４項ま
での浄化剤において、微生物群１０重量部に対し、糖類
５〜１０重量部、窒素系栄養源０．２〜５重量部、珪酸
カルシウムの多孔体２０〜１００重量部、水１０００〜
５０００重量部の混合物を、温度２０℃〜４０℃、酸素
供給量０．０３リットル／分〜０．１０リットル／分で
３６時間培養することによって得られた培養物を凍結乾
燥した粉末であることにより、好気性の微生物群に富ん
だ優れた浄化剤が得られ、しかも取り扱いが容易であ
る。Further, in the present invention, the purifying agent according to any one of the above-mentioned items 1 to 4, wherein 5 to 10 parts by weight of saccharide, 0.2 to 5 parts by weight of nitrogen-based nutrient, 20 to 100 parts by weight of a porous material of calcium silicate,
A freeze-dried powder of a culture obtained by culturing 5000 parts by weight of a mixture at a temperature of 20 ° C. to 40 ° C. and an oxygen supply of 0.03 liter / min to 0.10 liter / min for 36 hours. Thereby, an excellent purifying agent rich in aerobic microorganisms can be obtained, and the handling is easy.
【００２１】ここで好気性の微生物群に富んだ優れた浄
化剤を製造する場合において、糖類は、特に限定される
ものでなく、何でも良いが、グルコースが好ましい。糖
類の混合割合は、微生物群１０重量部に対し、５〜１０
重量部であり、糖類の量が５重量部未満のときは、微生
物の生育を効果的に促進しない。また糖類の量が１０重
量部を越えるとバスチル属やシュードモナス属の菌数が
増えすぎて他種の菌との共存が不可能となる。また窒素
栄養源として用いられる水溶性窒素化合物は、特定のも
のでなく、通常用いられるものでよく、ここでは塩化ア
ンモニウム、硫安等が挙げられる。この水溶性窒素化合
物の添加量が、微生物群１０重量部に対し、０．２重量
部未満のときには、微生物の生育を効果的に促進しな
い。また５重量部を越えると、バスチル属やシュードモ
ナス属の菌数が増えすぎて他種の菌とのバランスが保て
ないので、他種の菌との共存が不可能となる。In the case of producing an excellent purifying agent rich in aerobic microorganisms, the saccharide is not particularly limited and may be any one, but glucose is preferred. The mixing ratio of the saccharide is 5 to 10 with respect to 10 parts by weight of the microorganism group.
When the amount is less than 5 parts by weight, the growth of microorganisms is not effectively promoted. On the other hand, if the amount of the saccharide exceeds 10 parts by weight, the number of bacteria of the genus Bacillus or Pseudomonas will increase too much and it will be impossible to coexist with bacteria of other species. The water-soluble nitrogen compound used as a nitrogen nutrient is not limited to a specific one but may be a commonly used one, and examples thereof include ammonium chloride and ammonium sulfate. When the amount of the water-soluble nitrogen compound is less than 0.2 parts by weight with respect to 10 parts by weight of the microorganism group, the growth of the microorganism is not effectively promoted. If the amount exceeds 5 parts by weight, the number of bacteria of the genus Bastil and the genus Pseudomonas will increase too much to keep a balance with other species of bacteria, so that coexistence with other species of bacteria will be impossible.
【００２２】更に珪酸カルシウムの多孔体は、微生物の
担体として用いられるもので、微生物の増殖環境に適し
ているが、この珪酸カルシウムの多孔体の量が、微生物
群１０重量部に対し２０重量部未満のときは、微生物の
生育・増殖に効果的ではなく、また珪酸カルシウムの多
孔体の量が、１００重量部を越えると、他のものとのバ
ランス上好ましくない。水の混合量は、１０００〜５０
００重量部の範囲内で、微生物の生育に好適であり、使
用する水としては、脱イオン水や蒸留水が好ましい。Further, the porous material of calcium silicate is used as a carrier for microorganisms and is suitable for the growth environment of microorganisms. However, the amount of the porous material of calcium silicate is 20 parts by weight for 10 parts by weight of microorganism group. When the amount is less than the above, it is not effective for the growth and growth of microorganisms, and when the amount of the porous material of calcium silicate exceeds 100 parts by weight, it is not preferable in terms of balance with others. The mixing amount of water is 1000 to 50
Within the range of 00 parts by weight, it is suitable for the growth of microorganisms, and the water used is preferably deionized water or distilled water.
【００２３】本発明の浄化剤の製造方法は、コンクリー
トミキサー等の通常の混合機を用いて、前記の浄化剤成
分である微生物群、腐植物質、フミン酸、フルボ酸、ゼ
オライト、糖類及び窒素系栄養源の所要量を混合するこ
とにより得られる。この際の混合時間は、均一に混合さ
れればよく、通常５分〜２０分、好ましくは８分〜１２
分混合することにより均一に混合される。The method for producing a purifying agent of the present invention is carried out by using a conventional mixer such as a concrete mixer, the microorganisms, humic acid, humic acid, fulvic acid, zeolite, saccharides and nitrogen, It is obtained by mixing the required amounts of nutrient sources. The mixing time at this time may be a uniform mixing, and is usually 5 minutes to 20 minutes, preferably 8 minutes to 12 minutes.
The mixture is mixed uniformly by minute mixing.
【００２４】[0024]
【実施例】以下に、本発明について実施例を挙げて更に
詳しく説明するが、本発明は、これらに限定されるもの
ではない。EXAMPLES The present invention will be described below in more detail with reference to examples, but the present invention is not limited thereto.
【００２５】〔実施例１〕Ｂ．ｓｕｂｔｉｌｉｓ〔ＩＡ
Ｍ，１１６８〕２ｇ、Ｓ．ｆａｅｃａｌｉｓ〔ＩＡＭ，
１１１９〕１ｇ、Ｅ．ｓａｋａｚａｋｉｉ〔ＩＡＭ，１
２６６０〕１ｇ、Ｒ．ｏｒｙｚａｅ〔ＩＡＭ，６００
６〕１ｇ、Ｎ．ｅｕｒｏｐａｅａ〔ＩＦＯ，１４２９
８〕、Ｎ．ａｇｉｌｉｓ〔ＩＦＯ，１４２９７〕１ｇ、
Ｐ．ｃａｒｙｏｐｈｉｌｌｉ〔ＩＦＯ，１２９５０〕１
ｇ、Ｃ．ｂｉａｚｏｔｅａ〔ＩＦＯ，１２６８０〕３ｇ
を混合し、２ｇのグルコースと２００ｇの滅菌水を混合
し、３０℃で６時間攪拌培養し、得られた培養液は微生
物群からなる。[Example 1] subtilis [IA
M, 1168] 2 g, S.M. faecalis [IAM,
1119] 1 g; sakazakii [IAM, 1
2660] 1 g, R.I. oryzae [IAM, 600
6] 1 g, N.P. europaea [IFO, 1429
8]; Agilis [IFO, 14297] 1 g,
P. carryophili [IFO, 12950] 1
g, C.I. biazotea [IFO, 12680] 3 g
Are mixed, 2 g of glucose and 200 g of sterile water are mixed, and the mixture is stirred and cultured at 30 ° C. for 6 hours. The obtained culture solution is composed of microorganisms.
【００２６】この微生物群１０重量部、グルコース５重
量部、塩化アンモニウム０．５重量部、珪酸カルシウム
多孔体３０重量部、水１５００重量部を混合し、温度３
０℃、酸素供給量０．０５リットル／分として３６時間
攪拌培養した後、凍結乾燥して粉末の浄化剤を得た。こ
の浄化剤は、好気性微生物と嫌気性微生物が共存してい
る。この浄化剤を１０重量部、腐植物質（総合研究所の
花畑からとった土を４４メッシュの篩で粗粒を除去して
えられたもの、総合研究所の所在地：埼玉県大宮市北袋
一丁目２９７番地三菱マテリアル株式会社内）を２０重
量部、フミン酸１０重量部、フルボ酸１０重量部、ゼオ
ライト４５重量部、グルコース４重量部、塩化アンモニ
ウム１重量部を混合し、ティーバックに入れた。総合研
究所の池（ＢＯＤ＝２０、ＣＯＤ＝１８、ＳＳ＝７０、
Ｔ−Ｎ＝０．６、Ｔ−Ｐ＝０．３の水）を試験用とし
た。この池は水量１００リットルなので本発明の浄化材
を０．５Ｋｇ計ってティーバックに入れて池底へつるし
た。このままの状態で、７日目、３０日目、６０日目及
び９０日目に試料を採取して、表２に示す水質検査を行
った。６０日経過後には池底がみえる程に透明性が改善
された。アオコの発生も見られなかった。得られた結果
を表２に示す。A mixture of 10 parts by weight of this microorganism group, 5 parts by weight of glucose, 0.5 parts by weight of ammonium chloride, 30 parts by weight of porous calcium silicate, and 1500 parts by weight of water was mixed.
After stirring and culturing for 36 hours at 0 ° C. and an oxygen supply amount of 0.05 liter / min, freeze-drying was performed to obtain a powdery purifying agent. In this purifying agent, aerobic microorganisms and anaerobic microorganisms coexist. 10 parts by weight of this purifying agent, humic substances (obtained by removing coarse particles from soil taken from the flower garden of the Research Institute, using a 44-mesh sieve. Location of the Research Institute: Kitabukuro, Omiya City, Saitama Prefecture) No. 297, Mitsubishi Materials Corporation), 20 parts by weight, humic acid 10 parts by weight, fulvic acid 10 parts by weight, zeolite 45 parts by weight, glucose 4 parts by weight, and ammonium chloride 1 part by weight were mixed and put in a tea bag. . Research Institute Pond (BOD = 20, COD = 18, SS = 70,
(TN = 0.6, TP = 0.3 water) was used for the test. Since this pond had a water volume of 100 liters, 0.5 kg of the purifying material of the present invention was weighed, placed in a tea bag, and suspended to the bottom of the pond. In this state, samples were collected on days 7, 30, 60, and 90, and water quality tests shown in Table 2 were performed. After 60 days, the transparency was improved so that the pond bottom could be seen. No blooms were found. Table 2 shows the obtained results.
【００２７】[0027]
【表２】 [Table 2]
【００２８】表２から明らかなように、本発明の浄化剤
を使用した場合には、各試験項目が湖沼基準値におい
て、水産３級（工業用水１級）また水道２、３級の基準
値を満たしていることがわかり、したがって、本発明の
浄化剤は、優れた浄化作用を有することがわかる。これ
に対して、市販の微生物製剤を用いた場合には、９０日
目で各項目で５倍以上の値を示し、本発明よりはるかに
浄化性能が劣るものである。As is clear from Table 2, when the purifying agent of the present invention was used, each test item was based on the reference value of lakes and marshes, the standard value of fisheries class 3 (industrial water class 1) and water supply class 2 and class 3. Therefore, it can be seen that the purifying agent of the present invention has an excellent purifying action. On the other hand, when a commercially available microbial preparation was used, the value of each item was 5 times or more on the 90th day, and the purification performance was far inferior to that of the present invention.
【００２９】〔実施例２〕実施例１の微生物群１０重量
部、総合研究所の花畑からとった土を４４メッシュの篩
で粗粒を除去した腐植物質を１００重量部、フミン酸５
０重量部、フルボ酸５０重量部、珪酸カルシウム多孔体
２００重量部、グルコース１０重量部、塩化アンモニウ
ム１重量部、水１５００重量部を混合し、温度３０℃、
酸素供給量０．０５リットル／分として３６時間培養
後、更に継続して、温度３０℃、酸素供給量なしで６０
時間培養を行ない、凍結乾燥後、粉末の浄化剤を得た。
この浄化剤は好気性微生物に富んでいる。これを用い
て、総合研究所の池で浄化テストを行った。池の水質は
ＢＯＤ＝３５、ＣＯＤ＝４０、ＳＳ＝８１、Ｔ−Ｎ＝
０．４５、Ｔ−Ｐ＝０．４であった。池の水量は、約１
００リットルである。本発明の浄化材を０．３Ｋｇはか
り、ティーバックに入れて、池底へつるした。２時間エ
アレーション（ティーバックの下に散気管を装備し
た。）２２時間静止の反復を行なった。７日目、３０日
目、６０日目及び９０日目に試料を採取して、表３に示
す水質検査を行った。３０日経過後には、池底が見える
程に透明性が改善された。得られた結果を表３に示す。Example 2 10 parts by weight of the microorganisms of Example 1, 100 parts by weight of humic substances obtained by removing coarse particles of soil taken from the flower field of the Research Institute, using a 44-mesh sieve, and humic acid 5
0 parts by weight, 50 parts by weight of fulvic acid, 200 parts by weight of porous calcium silicate, 10 parts by weight of glucose, 1 part by weight of ammonium chloride, and 1500 parts by weight of water, and the mixture was mixed at a temperature of 30 ° C.
After culturing for 36 hours at an oxygen supply rate of 0.05 liter / minute, the culture was further continued at a temperature of 30 ° C. and without oxygen supply at 60 ° C.
After culturing for a period of time and freeze-drying, a powdery purifying agent was obtained.
This purifying agent is rich in aerobic microorganisms. Using this, a purification test was performed in the pond of the Research Institute. The water quality of the pond is BOD = 35, COD = 40, SS = 81, TN =
0.45, T-P = 0.4. The amount of water in the pond is about 1
It is 00 liters. 0.3 Kg of the purifying material of the present invention was weighed, put in a tea bag, and hung to the bottom of the pond. A 2-hour aeration (equipped with a diffuser under the tee bag) was repeated for 22 hours. On days 7, 30, 60 and 90, samples were taken and the water quality tests shown in Table 3 were performed. After 30 days, the transparency was improved so that the bottom of the pond was visible. Table 3 shows the obtained results.
【００３０】[0030]
【表３】 [Table 3]
【００３１】表３から明らかなように、本発明の浄化剤
を使用した場合には、各試験項目が湖沼基準値におい
て、水道２級（水産２級）の基準値を満たしていること
がわかり、したがって、本発明の浄化剤は、優れた浄化
作用を有することがわかる。これに対して、市販の微生
物製剤を用いた場合には、９０日目で各項目で５倍以上
の値を示し、本発明よりはるかに浄化性能が劣るもので
ある。As is clear from Table 3, when the purifying agent of the present invention was used, it was found that each test item satisfies the standard value of the water supply class 2 (fisheries class 2) in the lake and marsh standard value. Therefore, it can be seen that the purifying agent of the present invention has an excellent purifying action. On the other hand, when a commercially available microbial preparation was used, the value of each item was 5 times or more on the 90th day, and the purification performance was far inferior to that of the present invention.
【００３２】〔実施例３〕実施例２において、珪酸カル
シウム多孔体にかえてゼオライトを用いた以外は、実施
例２と同様にして浄化剤を製造した。得られた浄化剤を
用いて実施例２と同様にして試験したところ、実施例２
と同様に市販の微生物製剤より優れた性能を有してい
た。またゼオライトとして、海緑石を用いた場合には、
単にゼオライトを使用したものより一段と優れていた。Example 3 A purifying agent was produced in the same manner as in Example 2, except that zeolite was used instead of the calcium silicate porous material. A test was conducted in the same manner as in Example 2 using the obtained purifying agent.
As well as the above, it had better performance than the commercially available microbial preparation. In addition, when marine stone is used as zeolite,
It was much better than just using zeolite.
【００３３】[0033]
【発明の効果】本発明の浄化剤は、微生物群、腐植物
質、フミン酸、フルボ酸、ゼオライト、糖類及び窒素系
栄養源からなることにより、即効性に優れ、十分な浄化
効果を有し、運転操作が容易であるという効果を奏する
ものである。The purifying agent of the present invention comprises a microorganism group, humic substances, humic acid, fulvic acid, zeolite, saccharides and nitrogen-based nutrients, and has excellent immediate action and a sufficient purifying effect. The effect is that the driving operation is easy.
【００３４】本発明では、前記の浄化剤に水５〜３０重
量部を混合し、温度２８℃〜４０℃、酸素供給量０．０
３リットル／分〜０．１０リットル／分で３６時間培養
した後、更に継続して温度３０℃、酸素供給を行わない
で６０時間培養し、得られた培養物を凍結乾燥して浄化
剤粉末を得ることにより、好気性微生物を多量に増殖す
ることができると共に嫌気性微生物の増殖も極力増殖さ
せることができ、両者の拮抗作用の少ない状態にするこ
とができるもので、両微生物群に富んだ優れた浄化剤が
得られ、しかも取り扱いが容易である。In the present invention, 5 to 30 parts by weight of water is mixed with the above-mentioned purifying agent, the temperature is 28 to 40 ° C., and the oxygen supply amount is 0.0
After culturing at 3 liters / minute to 0.10 liters / minute for 36 hours, the culture is further continued at a temperature of 30 ° C. for 60 hours without supplying oxygen, and the obtained culture is lyophilized to obtain a purifying agent powder. As a result, a large amount of aerobic microorganisms can be proliferated, and at the same time, the growth of anaerobic microorganisms can be proliferated as much as possible. An excellent purifying agent is obtained, and the handling is easy.
【００３５】更に、本発明において、微生物群がバチル
ス属、ストレプトコッカス属、エンテロバクター属、リ
ゾープス属、ニトロソモナス属、ニトロバクター属、シ
ュードモナス属及びセルロモナス属を含むことにより、
浄化剤の性能が優れ、即ち即効性に優れ、十分な浄化効
果を有する浄化剤が得られる。Furthermore, in the present invention, the microorganisms include Bacillus, Streptococcus, Enterobacter, Rhizopus, Nitrosomonas, Nitrobacter, Pseudomonas, and Cellulomonas.
A purifying agent having excellent purifying performance, that is, excellent immediate effect, and having a sufficient purifying effect can be obtained.
【００３６】更に本発明の第１項〜第４項までの浄化剤
において、微生物群１０重量部に対し、糖類５〜１０重
量部、窒素系栄養源０．２〜５重量部、珪酸カルシウム
の多孔体２０〜１００重量部、水１０００〜５０００重
量部の混合物を、温度２０℃〜４０℃、酸素供給量０．
０３リットル／分〜０．１０リットル／分で３６時間培
養することによって得られた培養物を凍結乾燥した粉末
であることにより、好気性の微生物群に富んだ優れた浄
化剤が得られ、しかも取り扱いが容易である。更にまた
本発明において、ゼオライトが、沸石又は海緑石を含有
していることにより微生物群の固定を良好にすることが
できる。Further, in the purifying agent according to any one of the first to fourth aspects of the present invention, 5 to 10 parts by weight of a saccharide, 0.2 to 5 parts by weight of a nitrogen-based nutrient, and A mixture of 20 to 100 parts by weight of a porous body and 1000 to 5000 parts by weight of water was prepared at a temperature of 20 to 40 ° C. and an oxygen supply amount of 0 to 100 parts by weight.
By using a freeze-dried powder of a culture obtained by culturing at a rate of 03 l / min to 0.10 l / min for 36 hours, an excellent purifying agent rich in aerobic microorganisms can be obtained. Easy to handle. Furthermore, in the present invention, since the zeolite contains zeolite or chlorite, the fixation of microorganisms can be improved.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 ＦＩ Ｃ１２Ｒ 1:46 1:01 1:845 1:38） ──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 6 Identification code FI C12R 1:46 1:01 1: 845 1:38)
Claims (6)
酸、ゼオライト、糖類及び窒素系栄養源からなる浄化
剤。1. A purifying agent comprising microorganisms, humic substances, humic acid, fulvic acid, zeolite, saccharides and nitrogen-based nutrients.
腐植物質０．５〜３重量部、フミン酸０．１〜１．０重
量部、フルボ酸０．１〜１．０、ゼオライト０．１〜１
０重量部、糖類（糖質）０．０５〜５重量部、窒素系栄
養源０．０１〜２重量部からなることを特徴とする請求
項１に記載の浄化剤。2. The method according to claim 1, wherein the proportion is 0.05 to 0.3 parts by weight of the microorganism group.
Humic substance 0.5-3 parts by weight, humic acid 0.1-1.0 parts by weight, fulvic acid 0.1-1.0, zeolite 0.1-1
The purifying agent according to claim 1, comprising 0 parts by weight, 0.05 to 5 parts by weight of a saccharide (saccharide), and 0.01 to 2 parts by weight of a nitrogen-based nutrient.
部を混合し、温度２８℃〜４０℃、酸素供給量０．０３
〜０．１０リットル／分で３６時間培養した後、更に継
続して温度２８℃〜４０℃、酸素供給を行わないで６０
時間培養した培養物を凍結乾燥した粉末であることを特
徴とする浄化剤。3. The purifying material according to claim 2, wherein 5 to 30 parts by weight of water is mixed, the temperature is 28 to 40 ° C., and the oxygen supply amount is 0.03.
After culturing for 36 hours at ０．１0.10 liter / min, the temperature is further continued at 28 ° C. to 40 ° C. for 60 hours without supplying oxygen.
A purifying agent, which is a powder obtained by freeze-drying a culture cultured for a period of time.
ス属、エンテロバクター属、リゾープス属、ニトロソモ
ナス属、ニトロバクター属、シュードモナス属及びセル
ロモナス属を含むことを特徴とする請求項１乃至請求項
３に記載の浄化剤。4. The microorganism according to claim 1, wherein the microorganisms include Bacillus, Streptococcus, Enterobacter, Rhizopus, Nitrosomonas, Nitrobacter, Pseudomonas and Cellulomonas. Purifier.
重量部、窒素系栄養源０．２〜５重量部、珪酸カルシウ
ムの多孔体２０〜１００重量部、水１０００〜５０００
重量部の混合物を、温度２８℃〜４０℃、酸素供給量
０．０３〜０．１０リットル／分で３６時間培養するこ
とによって得られた培養物を凍結乾燥した粉末であるこ
とを特徴とする請求項１又は請求項４に記載の浄化剤。5. A saccharide of 5 to 10 parts per 10 parts by weight of a microorganism group.
Parts by weight, 0.2 to 5 parts by weight of a nitrogen-based nutrient, 20 to 100 parts by weight of a porous material of calcium silicate, 1000 to 5000 parts of water
A culture obtained by culturing a mixture of parts by weight at a temperature of 28 ° C. to 40 ° C. and an oxygen supply amount of 0.03 to 0.10 l / min for 36 hours is a freeze-dried powder. The purifying agent according to claim 1.
いることを特徴とする請求項１乃至請求項５のいずれか
に記載の浄化剤。6. The purifying agent according to claim 1, wherein the zeolite contains zeolite or chlorite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9057697A JPH10277586A (en) | 1997-04-09 | 1997-04-09 | Purifying agent using microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9057697A JPH10277586A (en) | 1997-04-09 | 1997-04-09 | Purifying agent using microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10277586A true JPH10277586A (en) | 1998-10-20 |
Family
ID=14002267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9057697A Withdrawn JPH10277586A (en) | 1997-04-09 | 1997-04-09 | Purifying agent using microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10277586A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020029880A (en) * | 2002-02-26 | 2002-04-20 | 서영진 | Treatment method of drinking water for domestic animals |
| KR100420756B1 (en) * | 2001-08-08 | 2004-03-12 | 서희동 | Treatment method of sewage and wastewater by using activated humic substances |
| KR100424158B1 (en) * | 2001-08-22 | 2004-03-30 | 서희동 | Method of cultivation for humification bacteria by using activated humic substances |
| KR100471097B1 (en) * | 2002-03-13 | 2005-02-23 | 서희동 | Composting method of Garbage |
| KR101438380B1 (en) * | 2014-05-07 | 2014-09-05 | 석성기업주식회사 | Concrete block for purifying water |
| JP2015110760A (en) * | 2013-10-31 | 2015-06-18 | 恭子 柿澤 | Composition for cleaning |
| CN113526604A (en) * | 2021-05-24 | 2021-10-22 | 威海瑞能电力工程有限公司 | Sewage treatment agent and preparation method thereof |
-
1997
- 1997-04-09 JP JP9057697A patent/JPH10277586A/en not_active Withdrawn
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100420756B1 (en) * | 2001-08-08 | 2004-03-12 | 서희동 | Treatment method of sewage and wastewater by using activated humic substances |
| KR100424158B1 (en) * | 2001-08-22 | 2004-03-30 | 서희동 | Method of cultivation for humification bacteria by using activated humic substances |
| KR20020029880A (en) * | 2002-02-26 | 2002-04-20 | 서영진 | Treatment method of drinking water for domestic animals |
| KR100471097B1 (en) * | 2002-03-13 | 2005-02-23 | 서희동 | Composting method of Garbage |
| JP2015110760A (en) * | 2013-10-31 | 2015-06-18 | 恭子 柿澤 | Composition for cleaning |
| KR101438380B1 (en) * | 2014-05-07 | 2014-09-05 | 석성기업주식회사 | Concrete block for purifying water |
| CN113526604A (en) * | 2021-05-24 | 2021-10-22 | 威海瑞能电力工程有限公司 | Sewage treatment agent and preparation method thereof |
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