JPH10130158A - Immunity potentiation - Google Patents
Immunity potentiationInfo
- Publication number
- JPH10130158A JPH10130158A JP8303867A JP30386796A JPH10130158A JP H10130158 A JPH10130158 A JP H10130158A JP 8303867 A JP8303867 A JP 8303867A JP 30386796 A JP30386796 A JP 30386796A JP H10130158 A JPH10130158 A JP H10130158A
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- Prior art keywords
- extract
- minutes
- production
- interleukin
- immune system
- Prior art date
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、生薬である不老草
（Cistanche salsa)抽出物による免疫を賦活させる方法
に関するものであり、具体的には抗体の産生を増強し、
クラススイッチを促進し、さらにインターロイキン−６
の産生を増強する方法に関するものである。TECHNICAL FIELD The present invention relates to a method for stimulating immunity with an extract of crude grass (Cistanche salsa), which is a crude drug. Specifically, the present invention relates to a method for enhancing the production of antibodies,
Facilitates class switching and interleukin-6
And a method for enhancing the production of.
【０００２】[0002]
【従来の技術】不老草は、ハマウツボ科の多年生寄生草
本であり、生薬として用いられ、薬効は強壮、強精とさ
れているがその作用の詳細は明らかになっていない。2. Description of the Related Art Unequal grass is a perennial parasitic herb of the family Antrodiaceae and is used as a crude drug, and its medicinal properties are tonic and tonic, but the details of its action have not been clarified.
【０００３】一方、免疫機構は生体の防御機構の中核を
成しており、免疫系の賦活は生体にとって感染症等各種
疾病に対する予防・治療の面から極めて重要である。特
に、抗体産生の増強はウイルス、細菌、毒素等の外敵物
質を効果的に死滅、中和するために重要であり、またク
ラススイッチ（例えば、ＩｇＭタイプからＩｇＧタイプ
への変動）及びインターロイキン−６の産生の増強は早
期にそれらを死滅、中和するために重要であるが、抗体
産生、クラススイッチ及びインターロイキン−６の産生
を包括的かつ効果的に増強にする物質は、現在までに見
出されていない。On the other hand, the immune system is the core of the defense mechanism of the living body, and the activation of the immune system is extremely important for the living body from the viewpoint of prevention and treatment of various diseases such as infectious diseases. In particular, enhancement of antibody production is important for effectively killing and neutralizing foreign substances such as viruses, bacteria, toxins, etc., as well as class switching (eg, changing from IgM type to IgG type) and interleukin-. 6 are important to kill and neutralize them early, but substances that comprehensively and effectively enhance antibody production, class switching and interleukin-6 production have not yet been identified. Not found.
【０００４】[0004]
【発明が解決しようとする課題】本発明は、不老草抽出
物を用いることにより、生体防御機構の中枢である抗体
を早期にしかも多量に産生可能にすること及び有用な
（生体外）免疫系の確立を目的としたものである。DISCLOSURE OF THE INVENTION The present invention is to provide an early and large-scale production of antibodies, which are central to the biological defense mechanism, by using an extract of primroses and a useful (ex vivo) immune system. The purpose is to establish.
【０００５】本発明の発明者らは上記目的を達成するた
めに鋭意研究を重ねた結果、不老草抽出物を用いること
により、抗体産生を増強すること、クラススイッチを促
進すること及びインターロイキン−６の産生を増強する
ことに成功し、本発明を完成するに至った。[0005] The inventors of the present invention have conducted intensive studies to achieve the above-mentioned object. As a result, the use of an extract of Streptomyces herb enhances the production of antibodies, promotes class switching, and enhances the interleukin-specific activity. 6 was successfully enhanced, and the present invention was completed.
【０００６】これらの現象は生体への適用に加えて生体
外免疫系にも利用することができる。生体外免疫系は例
えば種々の抗原に対するヒトモノクローナル抗体産生ハ
イブリドーマを作製する場合のリンパ球の免疫に利用で
きる。特に、ヒトの場合にはヒト系での免疫は試験など
ができないことから、有用な生体外免疫系が求められて
いた。[0006] These phenomena can be utilized not only for application to living bodies but also for in vitro immune systems. The in vitro immune system can be used for immunization of lymphocytes, for example, when producing human monoclonal antibody-producing hybridomas against various antigens. In particular, in the case of humans, a immunization in a human system cannot be tested, and a useful in vitro immune system has been demanded.
【０００７】この発明は、このような課題を解決するも
のである。The present invention solves such a problem.
【０００８】[0008]
【課題を解決するための手段】この発明は、免疫系に不
老草抽出物を添加して免疫活性を増強させるものであ
る。SUMMARY OF THE INVENTION The present invention enhances the immune activity by adding an extract of Caesarea herbs to the immune system.
【０００９】この発明において使用する不老草は、全草
を利用することが可能であるが、茎あるいは根を用いる
のが望ましい。すなわち、通常、乾燥物として入手され
る不老草の全草又は茎、根から任意に選んだ部位を用
い、必要により破砕機（チョッパー、グラインダな
ど）、すり鉢、薬研、乳鉢などを用い破砕し、破砕物又
は粉末とし、これに水、アルコール溶液などの溶媒を加
え抽出する。不老草と抽出する溶媒の比率は、抽出物が
所望の濃度となればよく、特定されるものではないが、
例えば破砕又は粉末とした不老草１部（重量部、以下同
じ）に対し、溶媒２０〜２０００部とする。抽出は、常
温にて行ってもよいが、所望の濃度とするのに時間がか
かるだけでなく、抽出の途中で雑菌が繁殖する危険があ
るため、６０°Ｃ以上で行うのが望ましい。なお、１０
０°Ｃおいて１０分間熱処理すると失活するので、温度
を高くした場合は、短時間で処理するようにする。すな
わち、６０°Ｃで２０〜１２０分、８０°Ｃで１０〜６
０分位の抽出が望ましい。Although the whole grass can be used as the primrose in the present invention, it is desirable to use a stem or a root. That is, usually, the whole grass or stem of the phytocelecum obtained as a dried product, using a site arbitrarily selected from the roots, if necessary, crushing using a crusher (chopper, grinder, etc.), a mortar, a pharmaceutical laboratory, a mortar, A crushed product or powder is obtained, and a solvent such as water or an alcohol solution is added to the crushed product or powder for extraction. Although the ratio of the solvent to extract the primroses is not limited as long as the extract has a desired concentration, it is not specified.
For example, the solvent is 20 to 2000 parts for 1 part of crushed or powdered primrose (part by weight, the same applies hereinafter). The extraction may be performed at room temperature, but it is preferable to perform the extraction at 60 ° C. or higher because not only takes time to obtain a desired concentration, but also there is a risk that various bacteria may grow during the extraction. In addition, 10
If heat treatment is performed at 0 ° C. for 10 minutes, it is deactivated. Therefore, when the temperature is increased, the treatment is performed in a short time. That is, 20 to 120 minutes at 60 ° C and 10 to 6 at 80 ° C.
Extraction of the 0 th quantile is desirable.
【００１０】抽出処理した後、濾過、デカンテーショ
ン、遠心分離などの公知の方法にて残渣を分離し、上澄
液からなる不老草抽出物を得る。この不老草抽出物は、
そのまま用いてもよいが、必要により、濃縮物又は乾燥
物として用いることも可能である。なお、濃縮又は乾燥
には、例えば、凍結濃縮、凍結乾燥、減圧濃縮、減圧乾
燥、噴霧乾燥、泡沫乾燥など公知の方法が用いられる
が、高温で長時間処理すると失活するおそれがあるの
で、なるべく低い温度で処理するのが望ましい。[0010] After the extraction treatment, the residue is separated by a known method such as filtration, decantation, centrifugation, etc., to obtain an extract of an old grass comprising a supernatant. This extract of primrose is
It may be used as it is, but if necessary, it may be used as a concentrate or a dried product. In addition, for the concentration or drying, for example, known methods such as freeze concentration, freeze drying, concentration under reduced pressure, drying under reduced pressure, spray drying, and foam drying are used. It is desirable to process at a temperature as low as possible.
【００１１】なお、不老草抽出物に含まれる活性を有す
る物質の性質は、水及び８０％のメチルアルコールで抽
出されること、６０°Ｃ、３０分の熱処理では安定であ
るが、１００°Ｃ、１０分間の熱処理で失活する。The properties of the active substance contained in the extract of phytoaceae are that it can be extracted with water and 80% methyl alcohol, and that it is stable by heat treatment at 60 ° C. for 30 minutes, but it is stable at 100 ° C. Inactivated by heat treatment for 10 minutes.
【００１２】このようにして得た不老草抽出物を生体外
免疫系に加えたところ、抗体（ＩｇＧ及びＩｇＭ）の産
生が増し、しかも産生量が数倍上昇した。更に、不老草
抽出物を添加することによりＩｇＧ及びＩｇＭの産生が
早期に開始されることも明らかになった。また、ＩｇＭ
タイプからＩｇＧタイプへの変動のようなクラススイッ
チも促進されることが明らかになった。[0012] When the thus-obtained primrose extract was added to the in vitro immune system, the production of antibodies (IgG and IgM) increased, and the production amount increased several times. Furthermore, it was revealed that the production of IgG and IgM was started early by the addition of the phytospermum extract. In addition, IgM
It has been found that class switching, such as a change from type to IgG type, is also facilitated.
【００１３】ヒト系の免疫活性測定に用いる末梢血リン
パ球及びリンパ節リンパ球の調製方法は、例えば、ヒト
健常人末梢血の場合にはリンパ球分離液（和光純薬）を
用いて、リンパ節からの場合には遠心によりリンパ球を
分離し、Ｌ−ロイシルロイシンメチルエステルで処理し
て、細胞障害性Ｔ細胞及びサプレッサーＴ細胞を除去し
たリンパ球を調製した。A method for preparing peripheral blood lymphocytes and lymph node lymphocytes used for measuring the immunological activity of a human system is, for example, in the case of peripheral blood of a healthy human, using a lymphocyte separation solution (Wako Pure Chemical Industries, Ltd.). Lymphocytes were separated from the nodes by centrifugation and treated with L-leucylleucine methyl ester to prepare lymphocytes from which cytotoxic T cells and suppressor T cells had been removed.
【００１４】抗体及びインターロイキン−６の産生に及
ぼす不老草抽出物の影響は、５×１０5個の末梢血リン
パ球あるいはリンパ節リンパ球を、例えば２４穴プレー
トにてインターロイキン−２（１００ユニット／ｍ
ｌ）、２−メルカプトエタノール（５０μＭ）及び牛胎
児血清（１０％）を含むＥ−ＲＤＦ（全量１５００μｌ
／穴）で培養し、培地中に不老草抽出物を添加すること
により調べた。[0014] Effect of antibodies and eternal youth grass extract on the production of interleukin-6, interleukin-2 (100 with 5 × 10 5 peripheral blood lymphocytes or lymph node lymphocytes, for example, in 24-well plates Unit / m
l), E-RDF containing 2-mercaptoethanol (50 μM) and fetal calf serum (10%) (total 1500 μl)
/ Well), and examined by adding an extract of perennial herb to the medium.
【００１５】[0015]
【実施例】以下、本発明を実施例により説明するが、本
発明は、これら実施例に限定されるものではない。EXAMPLES The present invention will be described below with reference to examples, but the present invention is not limited to these examples.
【００１６】実施例１ 乾燥した不老草の茎及び根の部分を乳鉢等で細かく破砕
して粉末とした。次いで、２ｇの破砕した不老草粉末を
８０°Ｃの温水（５００ｍｌ）で時々かきまわしつつ、
４０分間抽出処理した。４°Ｃにて１３，４００×ｇで
２０分間遠心処理して上清を集めて不老草抽出物を得
た。Example 1 The stems and roots of dried grass were finely crushed with a mortar or the like to obtain powder. Then, while stirring 2 g of the crushed flax powder with warm water (500 ml) of 80 ° C. occasionally,
Extraction treatment was performed for 40 minutes. The mixture was centrifuged at 13,400 × g for 20 minutes at 4 ° C., and the supernatant was collected to obtain an extract of an old grass.
【００１７】実施例２ 実施例１で調製した破砕した不老草粉末の２ｇを８０°
Ｃとした８０％アルコール水溶液に加え、時々かきまわ
しながら、３０分間抽出処理した。これを実施例１と同
様に４°Ｃにて１３，４００×ｇで２０分間遠心処理し
て上清を集めて不老草抽出物を得た。Example 2 2 g of the crushed grass powder prepared in Example 1 was added at 80 °
C was added to an 80% alcohol aqueous solution, and the mixture was extracted for 30 minutes while stirring occasionally. This was centrifuged at 13,400 × g for 20 minutes at 4 ° C. in the same manner as in Example 1, and the supernatant was collected to obtain an extract of an old grass.
【００１８】実施例３ リンパ節リンパ球を用い、上記培養液の１／３０容量の
実施例１にて調製した不老草抽出物を加え、不老草抽出
物のＩｇＭ産生に対する影響を調べた。ＩｇＭ濃度の測
定は、ＩｇＭに対する抗体を用いた酵素免疫測定法によ
り行った。Example 3 Using the lymph node lymphocytes, 1/30 volume of the above culture solution was added to the extract of Heterophytes prepared in Example 1, and the effect of the extract of Heterozea on IgM production was examined. The IgM concentration was measured by an enzyme immunoassay using an antibody against IgM.
【００１９】すなわち、ラビット抗ＩｇＭ抗体をイムノ
プレートにコートし、これに検体を加えて、検体中に含
まれているＩｇＭを抗ＩｇＭ抗体を介してイムノプレー
トに固定した。固定されたＩｇＭをペルオキシダーゼ標
識した抗ＩｇＭ抗体で挟み込み、ＩｇＭ量に相関して固
定されたペルオキシダーゼをＡＢＴＳ（２，２’−アジ
ノ−ビス（３−エチルベンゾチアゾリン−６−スルホン
酸））で青色に発色させ、その青色強度を測定すること
によりＭｇＩ濃度を測定した。That is, a rabbit anti-IgM antibody was coated on an immunoplate, a sample was added to the plate, and IgM contained in the sample was immobilized on the immunoplate via the anti-IgM antibody. The immobilized IgM was sandwiched between peroxidase-labeled anti-IgM antibodies, and the immobilized peroxidase was correlated with the amount of IgM, and the immobilized peroxidase was treated with ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) in blue. And the intensity of blue was measured to determine the MgI concentration.
【００２０】その結果、図１から明らかなように、不老
草抽出物により１０５個細胞あたりのＩｇＭの産生が早
期に始まり、しかも大きな産生増強が認められ、産生ピ
ークも非添加の場合に比べ早期であることが明らかにな
った。これらのことから、不老草はＩｇＭ産生増強作用
及び早期のＩｇＭ産生開始作用を有することが明らかに
なった。As a result, as shown in FIG. 1, IgM production per 10 5 cells was initiated early by the extract of primrose, and a large increase in production was observed. It turned out to be early. From these facts, it became clear that the primroses had an IgM production enhancing action and an early IgM production initiation action.
【００２１】実施例４ 実施例３と同様の条件で培養し、ＩｇＧ産生に対する影
響をを調べた。ＩｇＧ濃度の測定は、ＩｇＧに対する抗
体を用い実施例３の酵素免疫測定法に準じ測定した。Example 4 The cells were cultured under the same conditions as in Example 3, and the effect on IgG production was examined. The IgG concentration was measured according to the enzyme immunoassay of Example 3 using an antibody against IgG.
【００２２】すなわち、実施例２で用いたラビット抗Ｉ
ｇＭ抗体に代えてラビット抗ＩｇＧ抗体を用いて同様に
処理して測定した。That is, the rabbit anti-I used in Example 2
A similar treatment was carried out using a rabbit anti-IgG antibody instead of the gM antibody, and the measurement was performed.
【００２３】その結果、図２から明らかなように、不老
草抽出物により１０５個細胞あたりのＩｇＧの産生が早
期に始まり、しかも大きな産生増強が認められ、産生ピ
ークも非添加の場合に比べ早期であることが明らかにな
った。これらのことから、不老草はＩｇＧ産生増強作用
及びＩｇＭタイプからＩｇＧタイプへの迅速なクラスス
イッチ作用を有することが明らかになった。As a result, as shown in FIG. 2, the production of IgG per 10 5 cells was initiated early by the extract of S. cerevisiae, and the production was greatly enhanced. It turned out to be early. From these facts, it was revealed that the primrose has an IgG production enhancing action and a rapid class switching action from IgM type to IgG type.
【００２４】実施例５ 末梢血リンパ球を用いて、不老草抽出物のインターロイ
キン−６の産生に対する影響を調べた。インターロイキ
ン−６濃度の測定は、インターロイキン−６に対する抗
体を用いた酵素免疫測定法により行った。Example 5 Using peripheral blood lymphocytes, the effect of an extract of Styrax on the production of interleukin-6 was examined. The measurement of the interleukin-6 concentration was performed by an enzyme immunoassay using an antibody against interleukin-6.
【００２５】すなわち、抗インターロイキン−６マウス
モノクロナール抗体をイムノプレートにコートし、これ
に検体を加え、検体中に含まれるインターロイキン−６
を抗インターロイキン−６抗体を介してイムノプレート
に固定した。固定化されたインターロイキン−６をペル
オキシダーゼ標識した抗インターロイキン−６抗体で挟
み込み、インターロイキン−６量と相関して固定された
ペルオキシダーゼをＡＢＴＳ（２，２’−アジノ−ビス
（３−エチルベンゾチアゾリン−６−スルホン酸））で
青色に発色させ、その青色強度を測定することによりイ
ンターロイキン−６濃度を測定した。That is, an anti-interleukin-6 mouse monoclonal antibody was coated on an immunoplate, a specimen was added thereto, and interleukin-6 contained in the specimen was added.
Was immobilized on an immunoplate via an anti-interleukin-6 antibody. The immobilized interleukin-6 was sandwiched between peroxidase-labeled anti-interleukin-6 antibodies, and the immobilized peroxidase was correlated with the amount of interleukin-6 and the ABTS (2,2'-azino-bis (3-ethylbenzo) A blue color was developed with thiazoline-6-sulfonic acid)), and the concentration of interleukin-6 was measured by measuring the blue intensity.
【００２６】その結果、図３に示したように不老草抽出
物はインターロイキン−６の産生を増強する活性を有す
ることが明らかになった。インターロイキン−６は成熟
したＢ細胞から血漿 Ｂ細胞への分化を誘導する因子と
考えられていることから、不老草は抗体産生細胞である
血漿Ｂ細胞への分化誘導を間接的に行うと考えられる。As a result, as shown in FIG. 3, it was revealed that the extract of Caesarea herb has an activity to enhance the production of interleukin-6. Since interleukin-6 is considered to be a factor that induces the differentiation of mature B cells into plasma B cells, it is thought that primroses indirectly induce differentiation into plasma B cells that are antibody-producing cells. Can be
【００２７】参考試験 リンパ節リンパ球を培養した場合の不老草の生細胞数に
対する影響を調べた。培養液としては上記の培養液を用
い、培養液の１／３０容量の不老草抽出物を添加した。
生細胞数はトリパンブルーを用いた色素排除法により、
色素で染色されない細胞数を測定することにより行っ
た。Reference Test The effect of the culture of lymph node lymphocytes on the viable cell count of primrose was examined. As the culture solution, the above culture solution was used, and 1/30 volume of the extract of primrose was added to the culture solution.
The viable cell count was determined by the dye exclusion method using trypan blue.
This was done by counting the number of cells that did not stain with the dye.
【００２８】図４に示したように、不老草抽出物を添加
しても培養された生細胞数には影響を与えなかった。As shown in FIG. 4, the addition of the extract of phytocele grass did not affect the number of cultured viable cells.
【００２９】[0029]
【発明の効果】本発明によれば、不老草抽出物により生
体防御機構の中枢であるＢリンパ球の分化誘導、早期の
クラススイッチ誘導及び抗体産生の増強が可能となり、
生体防御機構の中核をなす免疫系を強化できる。According to the present invention, it is possible to induce the differentiation of B lymphocytes, which is the center of the biological defense mechanism, to induce class switching at an early stage, and to enhance the production of antibodies, by using the extract of primroses.
It can strengthen the immune system that is the core of the biological defense mechanism.
【図１】 不老草抽出物のＩｇＭ産生に対する影響を測
定した結果を示すグラフ。縦軸は１日当たりのＩｇＭの
生産量（μｇ／１０５個）を、横軸は培養日数を示す。
なお、グラフの網掛け線は不老草抽出物を加えないと
き、白線は不老草抽出物を加えたときの結果である。BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a graph showing the results of measuring the effect of an Aspergillus niger extract on IgM production. The vertical axis indicates the amount of IgM produced per day (μg / 10 5 cells), and the horizontal axis indicates the number of culture days.
In addition, the shaded line of the graph is the result when the extract is not added, and the white line is the result when the extract is added.
【図２】 不老草抽出物のＩｇＧ産生に対する影響を測
定した結果を示すグラフ。縦軸は１日当たりのＩｇＧの
生産量（μｇ／１０５個）を、横軸は培養日数を示す。
なお、グラフの網掛け線は不老草抽出物を加えないと
き、白線は不老草抽出物を加えたときの結果である。FIG. 2 is a graph showing the results obtained by measuring the effect of an extract of Styrax grass on IgG production. The vertical axis indicates the amount of IgG produced per day (μg / 10 5 cells), and the horizontal axis indicates the number of days of culture.
In addition, the shaded line of the graph is the result when the extract is not added, and the white line is the result when the extract is added.
【図３】 不老草抽出物添加量のインターロイキン−６
産生に対する影響を測定した結果を示すグラフ。縦軸は
インターロイキン−６の生産量（μｇ／ｍｌ）を、横軸
は不老草抽出物の添加量を示す。FIG. 3 shows the amount of interleukin-6 added to an extract of phytocele grass
4 is a graph showing the results of measuring the effect on production. The vertical axis indicates the amount of interleukin-6 produced (μg / ml), and the horizontal axis indicates the amount of the extract of the primrose.
【図４】 不老草抽出物の生細胞数に対する不老草抽出
物の影響を測定した結果を示すグラフ。縦軸は生細胞数
（×１０５個／ｍｌ）を、横軸は培養日数を示す。な
お、黒丸は不老草抽出物を加えない場合、白丸は不老草
抽出物を加えた場合である。FIG. 4 is a graph showing the results obtained by measuring the effect of an extract of perennial herb on the viable cell count of the extract of perennial herb. The vertical axis indicates the number of living cells (× 10 5 cells / ml), and the horizontal axis indicates the number of days of culture. In addition, the closed circles indicate the case where the extract of the ageless plant was not added and the open circles indicate the case where the extract of the ageless plant was added.
Claims (6)
物を添加して免疫活性を増強させることを特徴とする免
疫を賦活させる方法。1. A method for stimulating immunity, comprising adding an extract of primrose (Cistanche salsa) to the immune system to enhance immune activity.
とである請求項第１項に記載の免疫を賦活させる方法。2. The method for stimulating immunity according to claim 1, wherein the enhancement of the immune activity is an enhancement of antibody production.
を促進することである請求項第１項に記載の免疫を賦活
させる方法。3. The method for activating immunity according to claim 1, wherein the enhancement of the immune activity is to promote class switching of the antibody.
の産生を増強することである請求項第１項に記載の免疫
を賦活させる方法。4. The immunological activity is enhanced by interleukin-6.
2. The method for stimulating immunity according to claim 1, wherein the method is to enhance the production of.
ルコール溶液により抽出したものである請求項第１項に
記載の免疫を賦活させる方法。5. The method for stimulating immunity according to claim 1, wherein the extract of the primrose is extracted with a water or / and alcohol solution of the primrose.
と８０％のメチルアルコールで抽出したものであり、な
おかつ活性が６０°Ｃ、３０分間の熱処理で安定で、１
００°Ｃ、１０分間の熱処理で失活する性質を有するも
のである請求項第５項に記載の免疫を賦活させる方法。6. Extraction with water or / and alcohol is carried out by extraction with water and 80% methyl alcohol, and its activity is stable by heat treatment at 60 ° C. for 30 minutes.
The method for activating immunity according to claim 5, wherein the method has the property of being inactivated by heat treatment at 00 ° C for 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8303867A JPH10130158A (en) | 1996-10-30 | 1996-10-30 | Immunity potentiation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8303867A JPH10130158A (en) | 1996-10-30 | 1996-10-30 | Immunity potentiation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10130158A true JPH10130158A (en) | 1998-05-19 |
Family
ID=17926236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8303867A Pending JPH10130158A (en) | 1996-10-30 | 1996-10-30 | Immunity potentiation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10130158A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004014410A1 (en) * | 2002-08-12 | 2004-02-19 | Kyung Hee University | Composition comprising the extract of cistanche deserticola y.c. ma showing enhancing activity of the neurite outgrowth and neurotrophic effects |
| JP2009057340A (en) * | 2007-09-03 | 2009-03-19 | Kyushu Univ | Cell proliferation inhibitor |
| CN112057522A (en) * | 2020-09-18 | 2020-12-11 | 江苏康缘药业股份有限公司 | Composition and preparation method thereof |
-
1996
- 1996-10-30 JP JP8303867A patent/JPH10130158A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004014410A1 (en) * | 2002-08-12 | 2004-02-19 | Kyung Hee University | Composition comprising the extract of cistanche deserticola y.c. ma showing enhancing activity of the neurite outgrowth and neurotrophic effects |
| JP2009057340A (en) * | 2007-09-03 | 2009-03-19 | Kyushu Univ | Cell proliferation inhibitor |
| CN112057522A (en) * | 2020-09-18 | 2020-12-11 | 江苏康缘药业股份有限公司 | Composition and preparation method thereof |
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